CN215906211U

CN215906211U - Pocket type amplification device

Info

Publication number: CN215906211U
Application number: CN202121985074.7U
Authority: CN
Inventors: 岂源
Original assignee: Beijing Qingfengtang Pharmaceutical Technology Co ltd
Current assignee: Beijing Qingfengtang Pharmaceutical Technology Co ltd
Priority date: 2021-07-30
Filing date: 2021-08-23
Publication date: 2022-02-25
Anticipated expiration: 2031-08-23
Also published as: CN215906212U; CN115786097A; CN115703991A; CN215667988U; CN215757272U; CN115703990A; CN115703989A; CN215667945U; CN115704049A

Abstract

The utility model discloses pocket type amplification equipment, wherein an amplification instrument comprises a bearing part, a heating part, a mounting frame body and a shell, wherein the bearing part and the heating part are arranged in the shell through the mounting frame body; the bearing part is provided with a bearing cavity position for placing a sample to be tested; the heating part is arranged around the periphery of the bearing cavity position for electric heating. The pocket type amplification equipment adopts the rechargeable lithium battery, the amplification instrument has small integral volume, can be carried in a pocket or in a bag without power supply, can be used in any occasion without being operated by professional persons, has clear and easily-judged results, is particularly suitable for families or sampling and inspection sites, and can obtain results 15 minutes after sampling.

Description

Pocket type amplification device

Technical Field

The utility model relates to the technical field of nucleic acid detection (DNA or RNA), in particular to a pocket type amplification device.

Background

Nucleic acid detection, as a method having high sensitivity and specificity, has been widely used in many fields such as disease diagnosis, food safety, infectious disease control, and the like. Detection of specific nucleic acid sequences in a simple manner can confer greater value in point-of-care (point-of-care) diagnostics and in point-of-care pathogen detection.

PCR (polymerase chain reaction) is a molecular biology technique for amplifying and amplifying a specific DNA fragment, which can be regarded as special DNA replication in vitro, and the biggest characteristic of PCR is that a trace amount of DNA can be greatly increased. However, PCR, a classical nucleic acid detection method, its inherent denaturation-renaturation-extension cycle, requires that it necessarily requires thermocycler equipment as a support, and professional laboratories also serve as a necessary condition because of aerosol contamination issues. Among them, the PCR extension technology platform, particularly the quantitative PCR (qpcr) method, is the most widely used pathogen detection method and is considered as a new gold standard test. qPCR provides a much shorter sample-to-result time (3 to 5 hours). However, although qPCR is widely accepted, it is limited by relying on standard reference substances (standard curves) for quantification. Unreliable and inconsistent commercial standard reference materials may also affect the accuracy of qPCR quantification. In addition, qPCR is susceptible to inhibition by naturally occurring substances in environmental samples (e.g., heavy metals and organic matter), leading to inaccurate or false negative results in target quantification. Therefore, the application of PCR in the fields of point-of-care rapid diagnosis (POCT), on-site rapid detection and the like is greatly limited. Compared to qPCR, recent digital PCR techniques have proven to be more robust solutions for the detection of microbial pathogens in environmental samples. Digital PCR is based on partitioning (partioning) and poisson statistics, so no external quantification standards need to be compared to quantify samples of unknown concentration. However, implementing digital PCR methods for use with point-of-use applications (point-of-use applications) can be challenging. This is because digital PCR requires expensive instrumentation (i.e., Bio-rad droplet digital PCR), a fully equipped laboratory environment, and trained technicians to perform the assays. These factors severely limit the accessibility and applications of digital PCR in resource-limited contexts.

To overcome these disadvantages, a large class of new methods for isothermal nucleic acid amplification has emerged, with LAMP being the most interesting and promising method.

Loop-mediated isothermal amplification (LAMP) is an alternative PCR nucleic acid amplification method developed by Nippon Rongyan chemical company, 2000. It is characterized by that it designs 4 specific primers for 6 regions of target gene, and under the action of strand displacement DNA polymerase (Bst DNA polymerase), it can implement constant-temp. amplification at 60-65 deg.C for 15-60 min⁹～10¹⁰The double nucleic acid amplification has the characteristics of simple operation, strong specificity, easy detection of products and the like. LAMP, as a molecular biology detection technology, has the characteristics of high specificity, high sensitivity, simplicity, convenience and low cost, and is widely used for diagnosis of clinical diseases, qualitative and quantitative detection of epidemic bacteria or viruses, sex identification of animal embryos and gene chips.

Thus, LAMP detection is a rapid, simplified, low cost assay for detecting microorganisms to provide molecular assays outside of a centralized laboratory, for example, where on-site point-of-use testing of environmental water in resource-limited locations is required.

LAMP detection is performed under isothermal conditions, which can be maintained in different instruments, such as a thermocycler and a water bath. The apparatus enables amplification of DNA/cDNA from a sample by heating a detection chamber inside the device to detect pathogens.

The nucleic acid amplification instrument performs amplification reaction by using strand displacement type DNA polymerase under the constant temperature condition, can realize amplification of 109-1010 times within 15-60 minutes, can generate a large amount of amplification products, namely magnesium pyrophosphate white precipitate, and can judge whether the target gene exists by observing the existence of the white precipitate by naked eyes. The LAMP method has the advantages of high specificity and high sensitivity, is very simple to operate, has low requirements on instruments in the application stage, can realize reaction by using a simple constant temperature device, is very simple in result detection, can directly observe white precipitates or green fluorescence by naked eyes, does not need to carry out gel electrophoresis observation results unlike the common PCR method, and is a method suitable for rapid detection on site and in basic level.

The isothermal amplification instrument can be applied to the fields of pathogenic microorganisms, species identification, animal epidemic diseases, transgenosis and the like, but the traditional isothermal amplification instrument is large in size and inconvenient to carry, so that the isothermal amplification instrument can only be used in a laboratory.

Because of the limitations of nucleic acid detection reagents and detection equipment based on amplification, the problem of extraction of nucleic acid or other samples to be detected cannot be solved by amplification operation in the existing detection, multiple uncovering is also needed in the amplification process, especially when an eight-connected tube is used as a reactor, and operation in a professional PCR laboratory is also needed to avoid pollution, so that the nucleic acid detection in the prior art cannot realize field sampling and field detection, especially a reactor which can directly complete reaction at one time after directly adding a sample is not available, and the traditional eight-connected tube or EP tube is still adopted, which is an important toggle that the nucleic acid detection cannot be well applied to POCT and the development and application of pathogenic microorganisms.

SUMMERY OF THE UTILITY MODEL

The technical problem to be solved by the utility model is as follows: aiming at the technical problems in the prior art, the utility model provides pocket type amplification equipment which can be carried in a pocket or in a bag, does not need to be electrified, can be used in any occasions, does not need to be operated by professionals, and has clear and easily-judged results.

In order to solve the technical problems, the technical scheme provided by the utility model is as follows:

a pocket type amplification device comprises a micro amplification instrument and a reactor, wherein the micro amplification instrument comprises a bearing part, a heating part, a mounting frame body and a shell, and the bearing part and the heating part are arranged in the shell through the mounting frame body; the bearing part is provided with a bearing cavity position for placing a sample to be tested; the heating part is arranged around the periphery of the bearing cavity position for electric heating, and the reactor is arranged on the bearing part.

As a further improvement of the above technical solution:

preferably, in the above technical solution, the bearing portion is provided with a bearing cylinder, and an inner cavity of the bearing cylinder forms a bearing cavity.

In the above technical solution, preferably, the heating portion includes a plurality of heating sheets, and the heating sheets are surrounded around the carrying cylinder, so that the carrying cylinder can be uniformly heated.

Preferably among the above-mentioned technical scheme, the amplification instrument still includes centrifugal part and the power supply unit that charges, centrifugal part is connected through a drive belt with the bearing cylinder, the power supply unit that charges is connected the energy with centrifugal part electricity, it is rotatory to drive the bearing cylinder from the centrifugal part, be equipped with the bearing between bearing cylinder and the installation support body. The centrifugal part and the bearing part are arranged side by side, the motor of the centrifugal part is small and exquisite, and the bearing cylinder can be driven to rotate centrifugally.

Preferably, in the above technical scheme, the housing is provided with an equipment port for placing a sample to be tested, and the equipment port is communicated with the bearing cavity. That is, the whole amplification instrument has only one inlet, is sealed in the shell, is not easy to enter dust and has long service life.

Preferably, among the above-mentioned technical scheme, the bearing cylinder is a hollow cylinder or the bottom is transparent material, the amplification instrument still includes the portion of shooing, the portion of shooing is located the bottom of bearing cylinder. The photographing part adopts a small-sized high-precision camera and is provided with a flash lamp, such as a camera of a mobile phone.

In the above technical solution, preferably, the reactor includes a sample adding part, a sample part and a reaction part, which are connected in sequence, wherein the sample adding part and the sample part are movably connected, the sample adding part is of a piston structure, a sample preserving fluid is pre-loaded in the sample part, and a reaction system is loaded in the reaction part; the connecting part of the reaction part and the sample part is provided with micropores, and the aperture of each micropore is not more than the capillary length of the sample liquid or the sample preservation liquid; under the sealed state of the reactor, the sample adding part moves towards the reaction part by external force to realize that the sample liquid passes through the micropores and is pressed into the reaction part under the sealed state.

In the above technical solution, preferably, at least one reaction chamber is arranged in the reaction part, an independent reaction system is preset in each reaction chamber, the micropores are arranged at the orifice of the reaction chamber, and each reaction chamber corresponds to one micropore.

Preferably among the above-mentioned technical scheme, the reaction portion is equipped with the reposition of redundant personnel stopper, the reposition of redundant personnel stopper adopts flexible material to make, the reposition of redundant personnel stopper is located the junction of reaction portion and sample portion, the reaction chamber is independently sealed through the reposition of redundant personnel stopper, the reposition of redundant personnel stopper is located to the micropore.

In the above technical scheme, preferably, the pore diameter of the micropores is 0.3-0.6 mm.

Compared with the prior art, the pocket type amplification equipment provided by the utility model has the following advantages:

(1) the pocket type amplification device adopts the rechargeable lithium battery, the whole length, width and height of the amplification instrument are about 7 x 2 x 6cm, the amplification instrument can be put in a pocket or carried in a bag, the power is not needed, the amplification instrument can be used in any occasions without operation of professionals, the result is clear and easy to judge, and the pocket type amplification device is particularly suitable for being used at home or in an acquisition and inspection site, and the result can be obtained 15 minutes after the sample is taken.

(2) The pocket type amplification equipment of the utility model heats through the heating part in the reaction process to maintain the constant temperature environment required by the amplification reaction; and vibrating and mixing the sample liquid and the reaction system by centrifugal rotation.

(3) The pocket type amplification equipment can be realized by matching simple heating equipment (even a vacuum cup) with the reactor of the utility model aiming at public health events, does not need to be operated by professionals, has clear and easily-judged result, is suitable for the requirements of various medical detection scenes at home and abroad at present, and can greatly improve the molecular diagnosis capability of the pocket type amplification equipment, particularly in relatively laggard areas.

Drawings

Fig. 1 is a schematic view of a state of use of an embodiment of the present invention (the housing is not shown).

FIG. 2 is a schematic diagram of an exploded structure of the amplification apparatus of the present invention.

FIG. 3 is a schematic view of the structure of the reactor of the present invention.

The reference numbers in the figures illustrate:

1. a bearing part; 11. a load bearing cavity; 12. a carrying cylinder; 2. a heating section; 3. a centrifuge section; 4. a motor; 5. a photographing part; 6. a housing; 61. a cover; 7. installing a frame body; 71. an upper mounting seat; 72. a base; 8. a reactor; 81. a sample section; 811. an outer tube; 812. an inner tube; 813. a seal member; 82. a sample addition part; 821. a concave plug; 822. a sample adding cap; 823. tearing the pull ring; 824. a sample adding plug; 825. a sample application cover; 83. a reaction section; 831. an outer sleeve; 832. an inner reaction tube; 833. a reaction chamber; 834. a shunt plug; 8341. a boss member; 8342. and (4) micro-pores.

Detailed Description

The present invention will be described more fully hereinafter with reference to the following examples. The following examples are illustrative only and are not to be construed as limiting the utility model.

The experimental procedures in the following examples are conventional unless otherwise specified. The experimental materials used in the following examples were all commercially available unless otherwise specified.

FIGS. 1 to 3 show an embodiment of a pocket type amplification apparatus of the present invention, wherein an amplification apparatus comprises a support part 1, a heating part 2, a mounting frame body 71 and a housing 6, the support part 1 and the heating part 2 are disposed in the housing 6 through the mounting frame body 71; the bearing part 1 is provided with a bearing cavity 11 for placing a sample to be tested; the heating part 2 is positioned below the bearing part 1 to electrically heat the bearing part 1, and the amplification instrument further comprises a rechargeable battery.

In this embodiment, the mounting frame body 71 includes an upper mounting seat 71 and a base 72, the carrying portion 1 is provided with a carrying cylinder 12, and the carrying cylinder 12 is mounted on the upper mounting seat 71. The inner cavity of the carrier cylinder 12 forms a carrier cavity 11. The heating part 2 includes a plurality of heating sheets, which are arranged around the circumference of the carrier cylinder 12, and ceramic heating sheets may be used.

In this embodiment, the amplification apparatus further includes a centrifugal part 3, the centrifugal part 3 is connected to the carrying cylinder 12 through a transmission belt, the centrifugal part 3 includes a motor 4 and a transmission shaft, the motor 4 is installed between the upper mounting seat 71 and the base 72, the transmission shaft extends out of the upper mounting seat 71, and the transmission belt is in meshing transmission with a gear on the transmission shaft. The bearing cylinder 12 is provided with a belt groove matched with the transmission belt, the motor 4 drives the transmission shaft, and the bearing cylinder 12 is rotated through the transmission belt. A bearing is arranged between the bearing cylinder 12 and the mounting frame body 71.

In this embodiment, the housing 6 is provided with an equipment port for placing a sample to be tested, and the equipment port is communicated with the bearing cavity 11. The volume of the amplification instrument is reduced, so that the amplification instrument is smaller.

In this embodiment, the carrying cylinder 12 is a hollow cylinder or the bottom is made of transparent material, the amplification apparatus further includes a photographing part 5, and the photographing part 5 is located at the bottom of the carrying cylinder 12.

The amplification instrument of the embodiment is also provided with a control module for controlling the operation of each part. The control module comprises a temperature sensor, a processing unit and a wireless unit. The temperature sensor is used for detecting the temperature of the bearing part 1 and transmitting the detection data to the processing unit. The camera is electrically connected with the processing unit. The wireless unit adopts a wireless network or a Bluetooth form, and transmits the photographing result of the processing unit to the host or the mobile phone, the technology of data transmission through the wireless unit in the prior art is very mature, and no more explanation is made here, and any structure capable of realizing the technology and being small can be used in the embodiment. The whole length, width and height of the amplification instrument are about 7 x 2 x 6cm, the amplification instrument can be put into a pocket or carried in the pocket, the power is not needed, the amplification instrument can be used in any occasions, the operation of professionals is not needed, the result is clear and easy to judge, the amplification instrument is particularly suitable for being used in families or sampling and detecting fields, and the result can be obtained 15 minutes after the sample is taken.

The utility model is also provided with a reactor 8 matched with the pocket type amplification equipment for use, the reactor 8 comprises a sample adding part 82, a sample part 81 and a reaction part 83 which are connected in sequence, wherein the sample adding part 82 and the sample part 81, and the sample part 81 and the reaction part 83 are movably connected to realize a sealing state, the sample adding part 82 is of a piston structure, a sample preserving fluid is pre-loaded in the sample part 81, and a reaction system is loaded in the reaction part 83; a micropore 8342 is arranged at the joint of the reaction part 83 and the sample part 81, and the pore diameter of the micropore 8342 is not more than the capillary length of the sample liquid or the sample preservation liquid; the surface tension of the liquid at the micropores is greater than its gravity. In this embodiment, the aperture of the micro-holes is 0.3-0.6 mm. In the sealed state of the reaction vessel 8, the sample addition part 82 moves toward the reaction part 83 by an external force, and the sample liquid is pressed into the reaction part 83 through the micropores 8342 in the sealed state. In order to be conveniently placed in the amplification instrument, the whole reactor 8 adopts a circular tube type structure, and particularly, the whole body is preferably made of transparent plastic materials.

In this embodiment, a plurality of reaction chambers 833 are provided in the reaction portion 83, independent reaction systems are provided in the reaction chambers 833 in advance, simultaneous detection of a plurality of detection items for the same sample can be realized, and the end portion of each reaction chamber 833 near the junction is provided with a micropore 8342, so that liquid cannot flow into the reaction chamber 833 from the sample portion 81 without an external force.

In this embodiment, the reaction portion 83 is provided with an inner reaction tube 832 and an outer sleeve 831 which are sleeved with each other, the inner reaction tube 832 is clamped in the outer sleeve 831, one end of the outer sleeve 831 is provided with an internal thread which is in coordination threaded connection with the sample portion 81, the reaction portion 83 is provided with a shunt plug 834, the shunt plug 834 is made of a flexible material, and the reaction portion 83 is made of a transparent rubber material. Shunt plug 834 is located the junction of reaction chamber 833 and sample portion 81, and one section upper portion of reaction portion 83 is the internal thread section, and the position of reaction chamber 833 is not located the junction of internal thread, and internal thread junction and reaction chamber 833 have been cut apart to shunt plug 834 promptly, and one side of shunt plug 834 is protruding in order to seal the accent in reaction chamber 833, and every is protruding to have a micropore 8342, and micropore 8342 runs through the arch, and the one side that shunt plug 834 faces sample portion 81 is the smooth surface. Reaction chamber 833 is sealed independently by a shunt plug 834. Before reaction, a reaction system is pre-buried in each reaction cavity 833, and the reaction system is sealed and buried, for example, a material which is not compatible with water and can change the form along with the change of temperature is used for sealing the reaction system. Each reaction chamber 833 can be provided with a different reaction system for detecting different items. Since the reactor 8 is small and compact as a whole, it is preferable to provide 4 reaction chambers 833.

In this embodiment, the outer sleeve 831 is a hollow tube, the inner reaction tube 832 is clamped in the outer sleeve 831, the inner reaction tube 832 is provided with one or more than one protruding ridge for positioning, the outer sleeve 831 is provided with a corresponding groove, and the inner reaction tube 832 and the outer sleeve 831 can be integrally formed or can be manufactured separately. In practice, because the reactor 8 is small, the outer sleeve 831 and the inner reaction tube 832 are typically manufactured in two parts and then assembled for ease of manufacture and ease of handling for embedding the reaction system.

In this embodiment, at least one protrusion or groove is formed on the outer surface of the outer sleeve 831, i.e. the outer surface of the reaction portion 83, for positioning the reactor 8 when it is placed in the loading chamber 11 of the amplification apparatus, and the loading chamber 11 is provided with a matching groove or protrusion.

In this embodiment, the sample adding part 82 includes a sleeved concave plug 821 and a sleeved sample adding cap 822, one section of the sample adding cap 822 connected with the sample part 81 is provided with an external thread connecting section, a rigid protruding part 8341 inserted into the sleeved concave plug 821 is arranged in the sample adding cap, the protruding part 8341 is cylindrical or cylindrical, the protruding part 8341 and the external thread connecting section are of concentric rings, and an annular cavity is arranged between the protruding part 8341 and the external thread connecting section. The concave plug 821 is made of transparent rubber materials, the concave plug 821 adopts a piston structure with a cylindrical cavity arranged inside, a circular ring extending outwards is arranged at the cavity opening of the top of the piston, the outer diameter of the circular ring is the same as that of the annular cavity, the diameter of the piston head at the other end is slightly larger than that of the inner cavity of the sample part 81, and the function of the piston head in the inner cavity of the sample part 81 is achieved. When the concave plug 821 is sleeved on the sample adding cap 822, the head part of the piston protrudes out of the sample adding cap 822, so that the sample adding part 82 and the sample part 81 can be connected in a sealing manner, and the sample liquid can enter the reaction part 83 by pressurizing the sample part 81 through rotating the sample adding part 82 in a single direction.

In this embodiment, the sample portion 81 is a coaxial sleeve, the inner tube 812 and the outer tube 811 are hollow tubes, one end of the outer tube 811 is provided with an internal thread, and the diameter of the section with the external thread is slightly smaller than the diameter of the middle section of the outer tube 811 and the section with the internal thread. The inner tube 812 has the same diameter as the small diameter of the outer tube 811. The sample part 81 is further provided with a sealing member 813, the sample storage solution is sealed in the cavity of the inner tube 812 by the sealing member 813 and the sample addition part 82 before the reaction, and the sealing member 813 is removed when the sample to be measured is put therein.

In this embodiment, the external thread of the sample addition cap 822 and the internal thread of the outer tube 811 are screwed into the cavity between the outer tube 811 and the inner tube 812 of the sample addition part 82, and the concave plug 821 is located in the inner tube 812 and seals the inner tube 812. The internal thread of the outer sleeve 831 of the reaction part 83 is in threaded connection with the external thread of the outer tube 811, and the end of the inner tube 812 at the connection extends outwards to form a limiting ring, so as to limit the position of the inner tube 812 in the outer tube 811 and prevent the sample preserving fluid or the sample fluid from entering the outer tube 811 into the compartment of the inner tube 812.

In this embodiment, the sample adding part 82 is further provided with a tearing ring 823, and the tearing ring 823 is used for limiting rotation of the sample adding part 82. Tearing ring 823 is located outer tube 811 of sample portion 81 and between application of sample cap 822, adopts the point formula of breaking to connect to tearing ring 823 outwards extends and is equipped with the operation section of being convenient for to tear. The outer circumference of one end of the cap 822 is provided with a plurality of ribs for facilitating the rotary pressurizing operation. The outer circumferences of the sample part 81 and the reaction part 83 are also provided with a plurality of friction ribs for easy operation during rotation.

Before the detection, the quick detection device of the utility model firstly pre-embeds the reaction systems in the reaction cavity 833 respectively, the reaction systems are in a freeze-drying state, the reaction systems are pre-embedded in the reaction cavity through an isolation layer (such as paraffin) which can be dissolved by heat, and the sample preservation solution is encapsulated in the cavity of the inner tube 812 in the sample part 81. The sample to be tested of the sampling swab is dipped in secretion or saliva, the reaction part and the sample part are opened, the sealing piece is removed, the swab section with the sample is arranged in the sample part 81 of the reactor, the reaction part 83 and the sample adding part 82 are screwed down, the tearing ring 823 is torn off, the sample liquid is pressurized by rotating the sample adding part 82, and the sample liquid is forced to flow into the reaction cavity 833 through the micropores 8342. And then the reactor 8 is placed in a bearing cavity 11 of the amplification instrument, a cover is covered, the heating part 2 works to heat the reactor, the heating temperature reaches 60-65 ℃, the heating part 2 stops heating, the centrifugal part 3 works to enable the reactor to rotate and carry out centrifugal motion, and the sample liquid and the reaction system vibrate and are uniformly mixed to react. When the temperature sensor detects that the temperature is lower than 60 ℃, the centrifugal part 3 stops working, the heating part 2 works, and the developed reactor is photographed after the reaction. In this embodiment, the rechargeable battery may be an expensive high-power battery, and when a lower-power battery is used to reduce the cost, the heating unit 2 and the centrifugal unit 3 are operated at intervals to ensure better normal operation of the heating unit 2 and the centrifugal unit 3. If the battery can supply power to the heating part 2 and the centrifugal part 3 at the same time, centrifugal rotation can be performed while heating.

The above embodiments are merely preferred embodiments of the present invention, which is not intended to limit the present invention in any way. Although the present invention has been described with reference to the preferred embodiments, it is not intended to be limited thereto. Therefore, any simple modification, equivalent change and modification made to the above embodiments according to the technical spirit of the present invention should fall within the protection scope of the technical scheme of the present invention, unless the technical spirit of the present invention departs from the content of the technical scheme of the present invention.

Claims

1. The pocket type amplification equipment is characterized by comprising a micro amplification instrument and a reactor, wherein the micro amplification instrument comprises a bearing part, a heating part, an installation frame body and a shell, and the bearing part and the heating part are arranged in the shell through the installation frame body; the bearing part is provided with a bearing cavity position for placing a sample to be tested; the heating part is arranged around the periphery of the bearing cavity position for electric heating, and the reactor is arranged on the bearing part.

2. The pocket type amplification apparatus according to claim 1, wherein said carrier section is provided with a carrier cylinder, and an inner cavity of said carrier cylinder constitutes a carrier cavity site.

3. The pocket amplification apparatus of claim 2, wherein said heating section comprises a plurality of heating strips, said heating strips being disposed around the circumference of the carrier cartridge.

4. The pocket type amplification device of claim 2, wherein the amplification device further comprises a centrifugal part and a charging power supply device, the centrifugal part is connected with the carrying cylinder through a transmission belt, the charging power supply device is electrically connected with the centrifugal part for supplying power, the centrifugal part drives the carrying cylinder to rotate, and a bearing is arranged between the carrying cylinder and the mounting frame body.

5. The pocket type amplification apparatus of claim 1, wherein said housing is provided with an equipment port for placing a sample to be tested, said equipment port being in communication with said load-bearing chamber.

6. The pocket type amplification apparatus of claim 2, wherein the carrying cylinder is a hollow cylinder or the bottom of the carrying cylinder is made of a transparent material, and the amplification apparatus further comprises a photographing part, wherein the photographing part is located at the bottom of the carrying cylinder.

7. The pocket type amplification apparatus of claim 1, wherein the reaction vessel comprises a sample addition part, a sample part and a reaction part connected in sequence, wherein the sample addition part and the sample part are movably connected, the sample addition part is of a piston structure, a sample preservation solution is pre-loaded in the sample part, and the reaction part is loaded with a reaction system; the connecting part of the reaction part and the sample part is provided with micropores, and the aperture of each micropore is not more than the capillary length of the sample liquid or the sample preservation liquid; under the sealed state of the reactor, the sample adding part moves towards the reaction part by external force to realize that the sample liquid passes through the micropores and is pressed into the reaction part under the sealed state.

8. The pocket type amplification apparatus of claim 7, wherein at least one reaction chamber is disposed in the reaction portion, an independent reaction system is preset in each reaction chamber, the micropores are disposed at the opening of the reaction chamber, and each reaction chamber corresponds to one micropore.

9. The pocket type amplification apparatus of claim 8, wherein the reaction portion is provided with a shunt plug, the shunt plug is made of a flexible material, the shunt plug is located at a connection position of the reaction portion and the sample portion, the reaction chamber is independently sealed by the shunt plug, and the micro-hole is provided in the shunt plug.

10. The pocket amplification apparatus of claim 8, wherein the pore diameter of the micropores is 0.3 to 0.6 mm.