CN216473214U

CN216473214U - Reagent kit and instant analysis device

Info

Publication number: CN216473214U
Application number: CN202122406670.1U
Authority: CN
Inventors: 梅哲; 张彤
Original assignee: Guangzhou Wondfo Biotech Co Ltd
Current assignee: Guangzhou Wondfo Biotech Co Ltd
Priority date: 2021-09-30
Filing date: 2021-09-30
Publication date: 2022-05-10
Anticipated expiration: 2031-09-30

Abstract

The utility model discloses a kit and a real-time analysis device, wherein the kit comprises a reaction bin and a sample container, the reaction bin comprises a bin body, a central piece and a reaction bin chamber, and the sample container is arranged in the central chamber. The kit can guide a biological sample into the central cavity, then the central cavity is sequentially communicated with different peripheral cavities through relative rotation between the bin body and the central part, different reaction mixtures can be arranged in the corresponding peripheral cavities according to the sequence for preparing the analyte sample, and the biological sample is automatically prepared into the analyte sample to be detected. And piston mechanism can go into the biological sample pump in the central cavity and mix in the peripheral cavity that corresponds, react, operation such as washing, can also be with corresponding liquid pump that the operation ended to go back to the central cavity in to and the liquid pump that obtains the preparation is gone into in the reaction storehouse, whole analyte sample manufacture process is very simple, and the preparation efficiency is high, satisfies the demand that detects immediately, and whole device simple structure.

Description

Reagent kit and instant analysis device

Technical Field

The utility model relates to the technical field of medical detection instruments, in particular to a kit and a real-time analysis device.

Background

Early detection of infection, such as at the time of an epidemic outbreak, affects not only the health of an individual, but also public health. Such detection requires a cost-effective, reliable and specific assay. Because of these needs, such assays have traditionally been performed in centralized laboratories, rather than on-the-fly. However, the instant analysis may bring the test to a test subject in the field or clinic, providing faster detection of the test subject and public health.

The Polymerase Chain Reaction (PCR) is a method in which DNA is denatured at a high temperature of 95 ℃ in vitro to become a single strand, a primer and the single strand are combined at a low temperature (usually about 60 ℃) according to the principle of base complementary pairing, the temperature is adjusted to the optimum reaction temperature (about 72 ℃) of DNA polymerase, and the DNA polymerase synthesizes a complementary strand along the direction from phosphate to pentose (5 '-3'). The PCR instrument manufactured based on polymerase is actually a temperature control device, can well control the denaturation temperature, the renaturation temperature and the extension temperature, and the PCR reaction uses high-temperature resistant Taq DNA polymerase to perform denaturation-annealing-extension reaction on DNA amplification solution and a water bath kettle after the reaction solution is added at one time, and the amplification reaction is generally completed within 2-4 hours. Amplification products are generally analyzed by electrophoresis, isotopes are not needed, radioactive pollution is avoided, and the method is easy to popularize. Current point-of-care testing equipment typically relies on a PCR instrument performed in the field to confirm the presence of suspected pathogens or pathogens. However, existing point-of-care testing equipment generally requires expensive and complex sample preparation steps, resulting in higher equipment costs and requiring specially trained personnel to perform accurate operations.

SUMMERY OF THE UTILITY MODEL

The embodiment of the utility model aims to: provided are a reagent kit and a real-time analysis device, which can perform real-time analysis on a biological sample and have the characteristics of low cost and simple operation.

To achieve the purpose, the embodiment of the utility model adopts the following technical scheme:

in one aspect, a kit is provided, comprising:

a reaction bin: the device comprises a bin body, a center piece and a reaction bin, wherein a mounting hole penetrates through the bin body along a first direction, a partition plate is arranged in the mounting hole and is adjacent to the lower end of the bin body, a plurality of barrel bodies are annularly fixed on the hole wall of the mounting hole, peripheral chambers are formed in the barrel bodies, the lower ends of the barrel bodies are connected with the partition plate, a first through hole is formed in the center of the partition plate, a piston mechanism capable of moving along the first direction is hermetically arranged in each peripheral chamber, a first connecting hole is formed in each peripheral chamber on the partition plate and corresponds to each peripheral chamber, the peripheral chambers are communicated with the first through holes, the center piece penetrates through the first through holes and is hermetically connected with the partition plate, the center piece and/or the bin body rotates around the center of the first through hole, and a central chamber is arranged in the center piece, the opening of the central cavity is positioned at the upper end of the central piece, a second communication hole communicated with the central cavity is formed in the central piece, the second communication hole is selectively communicated with one of the first communication holes, the reaction chamber is communicated with the central cavity and/or at least one peripheral cavity, the reaction chamber is positioned in the mounting hole and positioned between the central cavity and the peripheral cavity, a heating mechanism is arranged in the reaction chamber and/or the peripheral cavity, and a first notch is formed in the upper end of the central piece;

a sample container: the sample container sets up inside the central cavity, the sample container includes bottle and seal, the inside of bottle has the sample chamber that is used for holding sample solution, the upper end of sample chamber has the opening, and the lower extreme is provided with the valve body, the valve body includes disk seat and valve plug, valve plug selectivity shutoff the valve pocket of disk seat, the disk seat with the bottom fixed connection of sample chamber, the valve plug keeps away from the looks one side of sample chamber is provided with first arch, first arch with first notch cooperation is connected, rotates the centre piece, can drive the valve plug for the disk seat rotates, so that the inside and the outside intercommunication of sample chamber, the seal is used for the selectivity to seal the open end of sample chamber.

On the other hand, the instant analysis device comprises a device body, wherein the reagent kit is arranged on the device body, and the instant analysis device is further provided with a first driving piece and a second driving piece, the first driving piece is used for driving the piston mechanism of the reagent kit to move, and the second driving piece is used for driving the central piece of the reagent kit to rotate.

The embodiment of the utility model has the beneficial effects that: through the storehouse body that sets up to have a plurality of periphery cavities and the centerpiece that has the central cavity, can introduce into the central cavity with biological sample, then relative rotation between storehouse body and the centerpiece, make the central cavity communicate with different periphery cavities in proper order, can set up different reaction mixture according to the order of preparation analyte sample in corresponding the periphery cavity, for example the lysis buffer that sets gradually, combine buffer, first washing buffer, second washing buffer and elution buffer, make biological sample automatic preparation be the analyte sample, and then detect. And piston mechanism can go into the biological sample pump in the central cavity and mix in the peripheral cavity that corresponds, react, operation such as washing, can also be with corresponding the liquid pump that the operation ended to go back to the central cavity to and go into the reaction storehouse with the liquid pump that the preparation obtained in, whole analyte sample preparation process is very simple, and the preparation efficiency is high, can promote detection efficiency, satisfies the demand that detects immediately, and whole device simple structure. The detection is carried out by adopting an instant analysis device, and the detection result can be obtained within 10 minutes to 2 hours.

Drawings

The utility model is explained in more detail below with reference to the figures and examples.

Fig. 1 is an overall configuration diagram of an instant analysis apparatus according to an embodiment.

FIG. 2 is a block diagram of the instant analysis device with one of the protection doors removed.

FIG. 3 is a structural diagram of a reaction chamber according to an embodiment.

Fig. 4 is a perspective view of the cartridge body of the present embodiment.

Fig. 5 is a block diagram of another angle of the cartridge body of the embodiment.

Fig. 6 is a structural view of the center piece according to the embodiment.

FIG. 7 is a cross-sectional view of a centerpiece according to an embodiment.

FIG. 8 is a diagram of the first driver and piston mechanism according to one embodiment.

Fig. 9 is a schematic view of the connection of the second driving member to the center member according to the embodiment.

Fig. 10 is a structural view of the heating device and the cartridge body according to the embodiment.

FIG. 11 is a block diagram of a first seal according to an embodiment.

FIG. 12 is a block diagram of a second seal according to an embodiment.

Fig. 13 is a structural view of the lower cover according to the embodiment.

Fig. 14 is a structural view of the upper cover according to the embodiment.

Fig. 15 is a structural view of a heat conductor according to an embodiment.

Fig. 16 is a structural view of the piston rod according to the embodiment.

FIG. 17 is a block diagram of an angle of a sample container according to an embodiment.

FIG. 18 is a block diagram of another angle of a sample container according to an embodiment.

In the figure:

100. a reaction bin; 101. a barrel; 102. a peripheral chamber; 103. a center piece; 104. a first notch; 105. a third recess; 106. a bin body; 107. a first through hole; 108. a vent; 109. a first communication hole; 110. detecting a window; 111. a step surface; 112. a piston rod; 113. connecting grooves; 114. a first seal member; 115. a first insertion tube; 116. a second communication hole; 117. a second insertion tube; 118. a second seal member; 119. a lower cover; 120. an upper cover; 121. a second through hole; 122. a heat conductor; 123. a partition plate; 124. a fourth communication hole; 125. a second recess;

200. a sample container; 201. a bottle body; 202. a first protrusion; 203. a second protrusion; 204. a valve plug; 205. a valve seat; 206. A seal body; 207. an elastomeric seal;

300. a first driving member; 301. a first rotary motor; 302. a rack; 303. a connecting portion;

400. a second driving member; 401. rotating the spline; 402. a second rotary motor; 403. magnetically attracting the device;

500. a heating device; 501. a heat conductor interface;

600. a protective door;

700. a display.

Detailed Description

In order to make the technical problems solved, technical solutions adopted and technical effects achieved by the present invention clearer, the technical solutions of the embodiments of the present invention will be described in further detail below with reference to the accompanying drawings, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, unless expressly stated or limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, e.g., as meaning permanently connected, removably connected, or integral to one another; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

As shown in fig. 1 to 18, the present invention provides an instant analysis apparatus including an apparatus body, a detector, a scanner, and a printer. The device body is provided with a reagent cartridge, a second driving member 400, a first driving member 300 and a heating device 500. In this embodiment, the device body is provided with a plurality of reagent boxes, and the specific quantity of reagent boxes on the device body can be adjusted as required in a flexible manner during actual design. The point-of-care assay devices are primarily used for the analysis and detection of biological samples, including the detection and diagnosis of pathogens (e.g., infectious agents) or conditions (e.g., infections) in subjects.

Wherein, the kit comprises a reaction chamber 100 and a sample container 200. The reaction bin 100 comprises a bin body 106, a center piece 103 and a reaction bin chamber, wherein a mounting hole is formed in the bin body 106 in a penetrating mode along a first direction, and a partition board 123 is arranged in the mounting hole. The baffle 123 is adjacent to the lower extreme of storehouse body 106, and the pore wall of mounting hole is fixed with a plurality of barrels 101 in the annular, forms peripheral cavity 102 in the barrel 101, and the lower extreme and the baffle 123 of barrel 101 are connected, and first through-hole 107 is seted up at the center of baffle 123. The outer peripheral chamber 102 is provided with a piston mechanism which can move along a first direction in a sealing mode, a partition plate 123 is provided with a first communication hole 109 corresponding to each outer peripheral chamber 102, the first communication hole 109 is communicated with the outer peripheral chamber 102 and the first through hole 107, and the central piece 103 penetrates through the first through hole 107 and is connected with the partition plate 123 in a sealing mode. In this specification, the first direction refers to a vertical direction. The center piece 103 and/or the cartridge body 106 rotate about the center of the first through hole 107, so that the center piece 103 can rotate relative to the cartridge body 106. It should be noted that the center piece 103 can rotate relative to the cartridge body 106, in the first case, the cartridge body 106 is stationary, and the center piece 103 rotates around the center of the first through hole 107; in the second situation, the center piece 103 is static, and the bin body 106 rotates around the center of the first through hole 107; in the third case, the cartridge body 106 and the center member 103 rotate simultaneously about the center of the first through hole 107, but the rotation speeds of the two are different. A central chamber is arranged in the central member 103, the opening of the central chamber is positioned at the upper end of the central member 103, a second communication hole 116 communicated with the central chamber is arranged on the central member 103, and the second communication hole 116 is selectively communicated with one of the first communication holes 109. The center piece 103 and the bin body 106 are relatively rotated, so that the second communication hole 116 is selectively communicated with one of the first communication holes 109, and the liquid communication between the central chamber and the peripheral chamber 102 is opened or closed. The reaction chamber is communicated with the central chamber and/or at least one peripheral chamber 102, the reaction chamber is positioned in the mounting hole and positioned between the central chamber and the peripheral chamber 102, a heating mechanism is arranged in the reaction chamber and/or the peripheral chamber 102, and the upper end of the central piece 103 is provided with a first notch 104. Referring to fig. 17 and 18, a sample container 200 is disposed in the central chamber, the sample container 200 includes a vial 201 and a sealing body, the vial 201 has a sample chamber therein for containing a sample solution, in this embodiment, a biological sample is a sample solution. The upper end of the sample chamber is provided with an opening, the lower end of the sample chamber is provided with a valve body, the valve body comprises a valve seat 205 and a valve plug 204, the valve cavity of the valve seat 205 is selectively plugged by the valve plug 204, the valve seat 205 is fixedly connected with the bottom of the sample chamber, one side, away from the sample chamber, of the valve plug 204 is provided with a first protrusion 202, the first protrusion 202 is connected with a first notch 104 in a matched mode, the center piece 103 rotates and can drive the valve plug 204 to rotate relative to the valve seat 205, and therefore the interior and the exterior of the sample chamber are communicated. Specifically, the first protrusion 202 extends in the horizontal direction. When the valve cavity is closed, the biological sample inside the sample chamber is trapped inside the sample container 200. Preferably, there are two first recesses 104, and the two first recesses 104 are arranged at an angle of 180 degrees on the upper end surface of the central member 103. Correspondingly, two ends of the first protrusion 202 are respectively connected in the two first recesses 104. The seal body is used to selectively seal the open end of the sample chamber.

In this embodiment, the sample chamber of the sample container 200 is used to contain a biological sample. In specific use, a biological sample is placed in the sample container 200, the open end of the sample chamber is sealed by the sealing body, and the sample container 200 is inserted into the central chamber, so that the biological sample is isolated in the central chamber by the sample container 200, and the biological sample in the sample container 200 is prevented from being polluted. Opening the valve body enables the interior of the sample chamber to communicate with the peripheral chamber 102, thereby allowing the biological sample inside the sample chamber to flow out into the central chamber. The reaction mixture is provided in the outer peripheral chambers 102, and when the center member 103 and/or the cartridge body 106 rotate around the center of the first through hole 107, the central chambers communicate with the corresponding outer peripheral chambers 102 through the second communication holes 116 and the first communication holes 109, and moving the piston mechanism in the first direction can pump the reaction mixture in the outer peripheral chambers 102 into the central chambers and combine or react with the liquid in the central chambers. Through setting up the storehouse body 106 that has a plurality of periphery cavities 102 and the centre piece 103 that has the central cavity, can introduce biological sample into the central cavity, then relative rotation between storehouse body 106 and the centre piece 103, make the central cavity communicate with different periphery cavities 102 in proper order, can set up different reaction mixture according to the order of preparation analyte sample in the corresponding periphery cavity 102, for example the lysis buffer solution that sets gradually, combine the buffer solution, first washing buffer solution, second washing buffer solution and elution buffer solution, make biological sample automatic preparation be the analyte sample, and then detect. And piston mechanism can pump into the biological sample pump in the central cavity and mix in the peripheral cavity 102 that corresponds, react, operation such as washing, can also be with corresponding liquid pump that the operation ended to the central cavity in to and the liquid pump that obtains the preparation is gone into in the reaction storehouse, whole analyte sample manufacture process is very simple, and the preparation efficiency is high, can promote detection efficiency, satisfies the demand that detects immediately, and whole device simple structure. The detection is carried out by adopting an instant analysis device, and the detection result can be obtained within 10 minutes to 2 hours.

Specifically, the sealing body comprises a sealing body 206 and an elastic sealing member 207 wrapped around the edge of the sealing body 206, and the sealing body 206 is hermetically connected with the inner side wall of the sample chamber through the elastic sealing member 207. Preferably, the resilient seal 207 is in the form of a circular ring. The elastic sealing member 207 has good elasticity and sealing performance, and the elastic sealing member 207 is connected with the inner side wall of the sample chamber in a sealing mode, so that liquid leakage caused by a gap between the sealing body and the inner side wall of the sample chamber is avoided.

In order to facilitate installation of the elastic sealing element 207, a side surface in the thickness direction of the sealing body 206 is concavely provided with a limiting groove, the elastic sealing element 207 is installed in the limiting groove, and the elastic sealing element 207 is limited by the limiting groove, so that the position of the elastic sealing element 207 is fixed.

In order to make the second communication hole 116 and the first communication hole 109 be combined in a sealing manner, an elastic sealing ring, specifically, an O-ring, is provided at the orifice of the second communication hole 116.

In this embodiment, referring to fig. 4 and 5, six cylinder bodies 101 are annularly fixed on the hole wall of the mounting hole, so that six peripheral chambers 102 are annularly distributed in the interior of the cabin body 106, and the six peripheral chambers 102 are a first peripheral chamber, a second peripheral chamber, a third peripheral chamber, a fourth peripheral chamber, a fifth peripheral chamber and a sixth peripheral chamber in sequence. Wherein the interiors of the first, second, third, fourth and fifth peripheral chambers are all provided with a reaction mixture, while the sixth peripheral chamber is not provided with a reaction mixture, and the interior of the sixth peripheral chamber is provided with only a piston mechanism and air. Two reaction chambers are arranged in the mounting hole, a cylinder body 101 is arranged between the two reaction chambers, and the two reaction chambers are respectively communicated with a peripheral cavity 102 of the cylinder body 101 positioned between the two reaction chambers through a third communication hole. In this embodiment, the outer peripheral chamber 102 between the two reaction chambers 100 is a sixth outer peripheral chamber, and the sixth outer peripheral chamber is communicated with the reaction chamber through a third communication hole. When the sixth peripheral chamber is communicated with the central chamber, the sixth peripheral chamber, the reaction chamber 100 and the central chamber are communicated with each other, and the liquid inside the central chamber can be transferred into the reaction chamber 100 by the piston mechanism inside the sixth peripheral chamber. The number of reaction chambers is not limited to two, and in other embodiments, the number of reaction chambers may be one, three, four, or the like.

Specifically, the reaction mixture inside the first peripheral chamber comprises a lysis buffer, the reaction mixture inside the second peripheral chamber comprises a first elution buffer, the reaction mixture inside the third peripheral chamber comprises a second elution buffer, and the reaction mixture inside the fourth peripheral chamber comprises an elution buffer. A piston mechanism inside the sixth peripheral chamber is used to transfer liquid inside the subsequent central chamber into the reaction chamber. Of course, in other embodiments, specific reagents or gases may be placed in the first peripheral chamber, the second peripheral chamber, the third peripheral chamber, the fourth peripheral chamber, the fifth peripheral chamber, and the sixth peripheral chamber according to actual testing and analysis requirements.

The first through hole 107 provides a space for the central member 103 to pass through the partition 123, and when the central member 103 rotates around the center of the first through hole 107, in order to avoid the hole wall of the first through hole 107 from causing position interference on the central member 103 and ensure smooth rotation of the central member 103, the position where the central member 103 is matched with the first through hole 107 is cylindrical.

In this embodiment, the center piece 103 includes an upper portion and a lower portion connected to the upper portion, the upper portion has a larger cross-sectional dimension than the lower portion, a step surface 111 is formed between the upper portion and the lower portion, the step surface 111 abuts against the upper surface of the partition 123, and the step surface 111 prevents the center piece 103 from falling off the partition 123.

In order to reduce the liquid retention in the central chamber, the bottom of the central chamber is inclined, and the second communication hole 116 is communicated with the lowest position of the bottom of the central chamber, so that the liquid in the central chamber can be discharged through the second communication hole 116 to the maximum extent.

Referring to fig. 7, the second communication hole 116 is inclined downward from an end connecting the central chamber toward an end away from the central chamber. Specifically, the inside of upper portion has upper chamber, and the inside of lower part has the lower chamber with last chamber intercommunication, and upper chamber is cylindricly, and the chamber bottom of lower chamber inclines downwards towards the one end of keeping away from central cavity, forms the funnel structure. The central chamber of this structure facilitates the discharge of the internal biological sample through the second communication hole 116.

In this embodiment, referring to fig. 6, the lower end of the central member 103 is provided with a third recess 105, and the third recess 105 is used for connecting a second driving member 400 for driving the central member 103 to rotate. The second driving member 400 is provided with a third protrusion in fit connection with the third recess 105, and the second driving member 400 and the central member 103 are stably connected through the third protrusion in fit connection with the third recess 105.

Preferably, there are two third recesses 105, two third recesses 105 are disposed at an included angle of 180 degrees on the end surface of the lower end of the central member 103, and each third recess 105 has a corresponding third protrusion. Of course, the number of the third recesses 105 is not limited to two, and in practical design, the specific number of the third recesses 105 can be flexibly changed as needed.

Wherein, barrel 101 is cylindrical barrel, and blow vent 108 has been seted up to the upper end of many barrels 101, and blow vent 108 intercommunication periphery cavity 102 and mounting hole. The vent 108 can discharge or suck air into the peripheral chamber 102 when the piston mechanism moves in the peripheral chamber 102 to stabilize the air pressure inside the peripheral chamber 102, so that the piston mechanism moves smoothly in the peripheral chamber 102, thereby discharging or introducing liquid smoothly.

In this embodiment, a transparent detection window 110 is disposed on the sidewall of the chamber body 106 corresponding to the reaction chamber, the detection window 110 allows an analyte sample located in the reaction chamber to be detected by an optical signal, and the detector emits an optical signal to detect the analyte sample in the detection window 110. Optical signals include, but are not limited to, fluorescent signals, chemiluminescent signals, electrochemiluminescent signals, colorimetric signals, and the like.

In an embodiment, referring to fig. 11, a rubber first sealing element 114 is detachably disposed at the lower end of the bin body 106, the first sealing element 114 includes a first body, a first insertion tube 115 is disposed on the first body corresponding to the first through hole 107, a fourth communication hole 124 is disposed on the inner sidewall of the first insertion tube 115 corresponding to each first communication hole 109, and the outer sidewall of the center element 103 abuts against the inner sidewall of the first insertion tube 115 to realize rotational sealing. The first body is provided with a second insertion cylinder 117, the second insertion cylinder 117 is tightly inserted into the peripheral cavity 102, the inner side wall of the second insertion cylinder 117 is in sealing fit with the outer side wall of the piston rod 112 of the piston mechanism, and the second insertion cylinder 117 is provided with a fifth communication hole communicated with the first communication hole 109. The lower end opening of the peripheral chamber 102 is isolated from the external environment by the second cartridge 117 and the lower end of the central chamber is isolated from the external environment by the first cartridge 115, and since the first communication hole 109 communicates with the fifth communication hole, a passage is formed between the peripheral chamber 102 and the central chamber, through which liquid communication between the peripheral chamber 102 and the central chamber is possible.

Specifically, referring to fig. 12, a rubber second sealing member 118 is detachably disposed at the upper end of the cartridge body 106, the second sealing member 118 is used for sealing the upper end opening of the peripheral chamber 102, and the upper end of the peripheral chamber 102 is blocked from the external environment by the second sealing member 118.

Referring to fig. 14, the reaction chamber 100 further includes an upper cover 120, the upper cover 120 is disposed on a side of the second sealing member 118 away from the chamber body 106, a second through hole 121 is disposed on the upper cover 120 corresponding to the central chamber, and a second notch 125 connected to the sample container 200 is disposed in the second through hole 121. Correspondingly, the upper end of the vial 201 is provided with a second protrusion 203 connected with the upper cover 120 of the reaction chamber 100, after the sample container 200 passes through the second through hole 121, the second protrusion 203 is connected with the second notch 125 in a matching manner, and the upper cover 120 can support and limit the sample container 200 through the second notch 125.

Preferably, there are two second protrusions 203, two second protrusions 203 are distributed on the outer sidewall of the bottle body 201 at an included angle of 180 degrees, there are two second recesses 125, and the second recesses 125 correspond to the second protrusions 203 one by one. The sample container 200 is connected to the upper cover 120 by the two second protrusions 203, so that the connection stability of the sample container 200 to the central member 103 is improved.

Referring to fig. 13, the reaction chamber 100 further includes a lower cover 119, the lower cover 119 is disposed on a side surface of the first sealing element 114 away from the chamber body 106, the lower cover 119 is provided with a hole for the central element 103 and the piston mechanism to pass through, and the lower surface of the first body is attached to the upper surface of the lower cover 119. The lower cover 119 primarily provides mechanical protection for the first seal 114. Preferably, the lower cover 119 is integrally injection molded with plastic.

Wherein, heating mechanism is heat conductor 122, and heat conductor 122 is connected with the outside firing equipment 500 in storehouse body 106, and firing equipment 500 provides the heat source for heat conductor 122, generates heat through firing equipment 500, with heat transfer to heat conductor 122 on, and then heat conductor 122 sends the heat and heats reaction bin or periphery cavity 102.

In order to ensure the heating effect of the heat conductor 122, the heat conductor 122 is at least partially located in the outer peripheral chamber 102 or the reaction chamber, so that the heating body is in direct contact with the liquid inside the outer peripheral chamber 102 or the reaction chamber.

Specifically, referring to fig. 15, the heat conductor 122 is fixedly connected to the lower cover 119, the heat conductor 122 is composed of a first section and a second section connected to the first section, the first section is located above the second section, the second section has a slot clamped to the edge of the lower cover 119, and the first section passes through the first body and extends into the peripheral chamber 102 or the reaction chamber. The lower cover 119 provides a supporting and fixing force for the heat conductor 122 by utilizing the clamping groove of the heat conductor 122 to clamp the edge of the lower cover 119.

Wherein the first driving member 300 is used for driving the piston mechanism to move, and the second driving member 400 is used for driving the central member 103 to rotate. When the second driving member 400 drives the center member 103 to rotate, the storage body 106 and the center member 103 rotate relatively, and the second communication hole 116 on the center member 103 is selectively communicated with the first communication hole 109 on the cylinder 101, that is, the center chamber is selectively communicated with the outer peripheral chamber 102.

Specifically, referring to fig. 16, the upper end of the piston rod 112 has a piston head for sealing the peripheral chamber 102, and the lower end of the piston rod 112 has a connecting groove 113, the connecting groove 113 being connected to the first drive member 300. Specifically, the inner side wall of the peripheral chamber 102 is provided with an elastic seal portion by which the piston head is sealed inside the peripheral chamber 102. The first driver 300 is connected with the piston rod 112 by a connecting slot 113, the first driver 300 being capable of driving the piston mechanism to move in a first direction, i.e. in a vertical direction, in the peripheral chamber 102.

Further, referring to fig. 8, the first driving member 300 includes a first rotating motor 301 and a rack 302, an output shaft of the first rotating motor 301 is connected to the rack 302, the rack 302 can be driven to ascend and descend along a vertical direction by the output shaft of the first rotating motor 301, and a connecting portion 303 cooperatively connected with the connecting groove 113 is provided at a lower end of the rack 302. The connecting portion 303 of the rack 302 is connected to the connecting groove 113 of the piston rod 112, so that the first driving member 300 and the piston rod 112 are connected as a whole, and the lifting of the rack 302 drives the lifting of the piston rod 112.

In an embodiment, the piston rods 112 in all the piston mechanisms share one first driving member 300, the first driving member 300 is connected with a translation mechanism, the translation mechanism can move the first driving member 300 to the corresponding piston mechanism, and the connecting portion 303 on the rack 302 is connected with the connecting groove 113 on the corresponding piston rod 112 in a matching manner, so that the number of the first driving members 300 is effectively reduced, and the saving of equipment cost is facilitated.

In another embodiment, there is one first driver 300 per piston mechanism, and the rack 302 in each first driver 300 is connected to the piston rod 112 in the corresponding piston mechanism.

In order to facilitate the identification of the reaction chamber 100 and the sample container 200, identification codes for identification are provided on both the reaction chamber 100 and the sample container 200. The identification code is preferably a bar code. The corresponding reaction chamber 100 or sample container 200 can be identified by the scanner, which facilitates recording the detection result. In other embodiments, an identification code for identification may be provided only on the reaction chamber 100 or the sample container 200.

Referring to fig. 9, the second driving member 400 includes a rotating spline 401, a magnetic attraction device 403, and a second rotating motor 402, the rotating spline 401 has a third protrusion, the lower end of the central member 103 is provided with a third recess 105, the third protrusion is inserted into the third recess 105 in a matching manner, the second rotating motor 402 is located on a side of the rotating spline 401 away from the central member 103, an output shaft of the second rotating motor 402 passes through the magnetic attraction device 403 to be connected with the rotating spline 401, and the magnetic attraction device 403 can attract magnetic beads inside the central chamber. The output shaft of the second rotating motor 402 can drive the rotating spline 401 to rotate, and because the third protrusion on the rotating spline 401 is inserted into the third notch 105, the rotation of the rotating spline 401 drives the center piece 103 to rotate, so that the relative rotation between the bin body 106 and the center piece 103 is realized. It can be understood that, in the process of automatically manufacturing a biological sample into an analyte sample, magnetic beads are added to intermediate products for generating the analyte sample in the peripheral chamber 102, so that the magnetic beads are combined with the intermediate products to form magnetic bead adherents, and then the magnetic bead adherents are separated from waste generated in the formation process of the magnetic bead adherents, during the separation, the magnetic bead adherents need to be adsorbed inside the central chamber by the magnetic attraction device 403 on the second driving member 400, and meanwhile, under the cooperation of the piston mechanism inside the second peripheral chamber, the magnetic bead adherents are left in the central chamber while the waste is introduced into the corresponding peripheral chamber 102, so that the magnetic bead adherents and the waste are smoothly separated, so as to perform the next operation.

In practical use, the second driving member 400 is connected to a lifting assembly, and the lifting assembly can drive the second driving member 400 to lift in a vertical direction, so that the second driving member 400 has a lifting state and a descending state. When the second driving member 400 is in the raised state, the second driving member 400 is close to the central member 103, and at this time, the third protrusion on the second driving member 400 can be in fit connection with the third notch 105 on the central member 103, and the magnetic attraction device 403 of the second driving member 400 can attract the magnetic beads inside the central chamber; when the second driving member 400 is in the descending state, the second driving member 400 is far away from the central member 103, and the first magnetic attraction device 403 on the second driving member 400 releases the attraction force on the magnetic beads in the central chamber.

Referring to fig. 10, a heating device 500 is further provided in the instant analysis apparatus for connecting with the heating mechanism, and the heating device 500 provides a heat source for the heat conductor 122, and transfers heat to the heating mechanism, thereby heating the peripheral chamber 102 or the central chamber.

The heating apparatus 500 includes an apparatus body, and a heat conductor interface 501 and a heat sensor (not shown) are disposed on the apparatus body, and the heat conductor interface 501 can be selectively connected to the heat conductor 122. Specifically, the thermal conductor interface 501 is selectively connected to the second section of the thermal conductor 122. The device body provides a heat source for the heat conductor 122, the heat conductor interface 501 provides a connection space for the heat conductor 122, heat conduction is facilitated, and the heat sensor is used for detecting the temperature of the heat conductor 122. In order to reduce the heat dissipation on the device body, a thermal insulation layer is arranged on the periphery of the device body, and a thermal insulation layer heat conductor interface 501 and a thermal sensor are arranged on the thermal insulation layer.

Three heating mechanisms are provided, one for heating one of the peripheral chambers 102, e.g. for heating the elution buffer or lysis buffer inside the peripheral chamber 102, and the remaining two for heating the liquid inside the two reaction compartments for providing heat for the reaction inside the reaction compartments. In this embodiment, one of the heating mechanisms is used to heat the interior of the first peripheral chamber. The heating device 500 is also provided with three sets, and each set of heating device 500 corresponds to one set of heating mechanism.

In one embodiment, the instant analysis device further comprises a third driving member, and the third driving member is used for driving the bin body 106 to rotate around the center of the first through hole 107. The equipment body is arranged on the periphery of the bin body 106, when the third driving piece drives the bin body to rotate, the equipment body is selectively contacted with the heat conductor 122, and when the heat conductor interface surface 501 of the equipment body is contacted with the heat conductor 122, the heat of the equipment body can be transferred to the heat conductor 122, so that the heat conductor 122 can provide heat for the peripheral cavity 102 or the central cavity. Specifically, one of the three sets of heating devices 500 is capable of heating the thermal conductor 122 to a temperature of about 60 ℃ and the remaining two sets of heating devices 500 are capable of heating the thermal conductor 122 to a temperature of about 90 ℃. In particular use, the cartridge body 106 is rotated according to the desired temperature levels in the peripheral chambers 102 and the central chamber interior such that the respective heating apparatus 500 is in contact with the corresponding thermal conductor 122 to provide a particular temperature to the peripheral chambers 102 or the central chamber interior.

Specifically, the third driving member includes a third rotating motor, an output shaft of the third rotating motor is connected to the bin body 106 or the lower cover 119, and the output shaft of the third rotating motor rotates to drive the bin body 106 to rotate.

Specifically, the detector is disposed at the outer periphery of the chamber body 106, and when the analyte sample in the reaction chamber of the detector is needed to be detected, the chamber body 106 can be rotated to make the detection window 110 face the detector.

In another embodiment, the detector can also be translated to the position of the detection window 110, such that the detector can detect the analyte sample inside the reaction chamber through the detection window 110.

In one embodiment, the instant analysis device further comprises a printer for printing the detection result of the detector on the analyte sample, and the printer is configured to facilitate the detection result to be known by the detector.

Optionally, a protective door 600 is further disposed on the device body, and the protective door 600 can selectively cover the reagent cartridge in the device body. In order to facilitate the taking and placing of the reagent cartridge, the protective door 600 may be opened by a triggering device.

Optionally, the detector is connected with a buzzer, and when the detector detects the analyte sample, the buzzer sends out an alarm signal to remind the user to open the protection door 600 to recover the reaction chamber 100 of the reagent kit.

In another embodiment, a display 700 is further disposed on the device body, and the display 700 is used for displaying the detection result and electronically transmitting the detection result to other electronic devices through the display 700.

The present invention also provides a real-time detection method, which is used in the real-time analysis device described in any of the above embodiments, and includes the following steps:

step S10 is to introduce the biological sample into the sample container 200.

Step S20, inserting the sample container 200 inside the central chamber of the reaction chamber 100 and introducing the corresponding reaction mixture inside the peripheral chamber 102 in the reaction chamber 100. The sample container 200 effectively prevents contamination of the biological sample from the external environment and prevents transfer of the biological sample to the external environment.

Step S30, placing the reaction chamber 100 in a reagent box, driving the chamber body 106 of the reaction chamber 100 and the center piece 103 to rotate relatively, so that the central chamber is sequentially communicated with the different peripheral chambers 102, so that the reaction mixture corresponding to the interior of the peripheral chambers 102 can sequentially react with the solution in the central chamber according to the sequence for preparing the analyte sample, to obtain a first detection mixed solution, and then introducing the obtained first detection mixed solution into the interior of the reaction chamber 100 to react, to obtain the analyte sample. In this step, the center piece 103 can be rotated around the center of the first through hole 107, or the cartridge body 106 can be rotated around the center of the first through hole 107, or the center piece 103 and the cartridge body 106 can be rotated around the center of the first through hole 107 at the same time, and the above three manners can realize the relative rotation of the cartridge body 106 and the center piece 103.

Preferably, the specific manipulations for driving the relative rotation of the cartridge body 106 of the reaction cartridge 100 and the center piece 103 are: the center piece 103 is rotated in a clockwise direction. In other embodiments, the center piece 103 may also be rotated in a counterclockwise direction.

Step S40, the detection window 110 on the reaction chamber 100 detects the analyte sample inside the reaction chamber.

In the method, the bin body 106 and the central piece 103 are relatively rotated, so that the central chamber is sequentially communicated with different peripheral chambers 102, different reaction mixtures can be arranged in the corresponding peripheral chambers 102 according to the sequence for preparing the analyte samples, so that the biological samples are automatically prepared into the analyte samples, the analyte samples are led into the reaction chambers, and then the analyte samples can be detected. The method has the characteristics of simple and convenient operation, low requirement on operators and accurate detection result. By adopting the instant detection method, the detection result can be obtained within 10 minutes to 2 hours.

Wherein, each peripheral chamber 102 in the reaction chamber 100 is a first peripheral chamber, a second peripheral chamber, a third peripheral chamber, a fourth peripheral chamber, a fifth peripheral chamber and a sixth peripheral chamber, the reaction mixture in the first peripheral chamber comprises a lysis buffer, the reaction mixture in the second peripheral chamber comprises magnetic beads for capturing analyte molecules, the reaction mixture in the third peripheral chamber comprises a first washing buffer, the reaction mixture in the fourth peripheral chamber comprises a second washing buffer, the reaction mixture in the fifth peripheral chamber comprises an elution buffer, the bin body 106 and the center piece 103 are driven to rotate relatively, so that the central chamber sequentially and the first peripheral chamber, the second peripheral chamber, the third peripheral chamber, the fourth peripheral chamber, the fifth peripheral chamber and the sixth peripheral chamber are communicated, and the piston mechanism in the sixth peripheral chamber can guide the first detection mixed liquid in the central chamber into the reaction chamber.

Step S30 specifically includes the following steps:

step S31, communicating the central chamber with the first peripheral chamber to mix the biological sample inside the central chamber with the lysis buffer inside the first peripheral chamber, thereby obtaining a second detection mixture inside the central chamber. Before the central chamber is communicated with the first peripheral chamber, the second driving member 400 is raised, the third protrusion of the second driving member 400 is connected with the third notch 105 of the central member 103, and the second rotating motor 402 driving the second driving member 400 rotates the central member 103 at a proper angle to open the valve body of the sample container 200, so that the biological sample inside the sample chamber flows into the central chamber. The rotation angle of the rotating part can be flexibly adjusted according to the requirement, such as 5 degrees, 10 degrees, 20 degrees, 30 degrees and the like. After the biological sample in the sample chamber flows to the central chamber, the second driving member 400 is utilized to drive the central member 103 to rotate, so that the central chamber is communicated with the first peripheral chamber, the first driving member 300 is connected with the piston mechanism in the first peripheral chamber, the piston mechanism descends in the first peripheral chamber under the driving of the first driving member 300, the reaction mixture in the first peripheral chamber is pumped into the central chamber, so that the biological sample in the central chamber is mixed with the lysis buffer in the first peripheral chamber, and the second detection mixed solution is obtained in the central chamber.

In order to mix the biological sample inside the central chamber and the reaction mixture inside the first peripheral chamber sufficiently, the piston mechanism inside the first peripheral chamber may be driven by the first driving member 300 to ascend and descend multiple times to repeatedly mix the biological sample inside the central chamber and the lysis buffer inside the first peripheral chamber.

In order to improve the lysis effect of the lysis buffer, when the biological sample is mixed with the lysis buffer, the second detection mixture is heated to the first set temperature T1 by the heating mechanism, and is kept warm for a first duration S1. In the present embodiment, the first set temperature T1 is 60 ℃. In other embodiments, the first set temperature T1 and the first time length S1 are flexibly set as needed.

Step S32, communicating the central chamber with the second peripheral chamber, mixing the second detection mixed solution with the magnetic beads inside the second peripheral chamber, adhering the organic matter inside the second detection mixed solution with the magnetic beads to obtain a magnetic bead adhered matter, leaving the magnetic bead adhered matter in the central chamber, and introducing the waste generated in the process of forming the magnetic bead adhered matter into the second peripheral chamber by using the piston mechanism inside the peripheral chamber 102. The magnetic beads in the second peripheral chamber are in a powder state, and are mainly used for capturing nucleic acid in the second detection mixed liquid. Before the second detection mixed liquid is mixed with the magnetic beads in the second peripheral chamber, the heated second detection mixed liquid is cooled for several seconds, for example, 1s-10s, or more than 10s, so that the influence of high temperature on the formation of magnetic bead adhesion substances is avoided. Specifically, the central member 103 is rotated by the second driving member 400, and after the central chamber is communicated with the second peripheral chamber, the second driving member 400 is lowered, so that the magnetic attraction device 403 in the second driving member 400 is far away from the central member 103, thereby preventing the magnetic attraction device 403 from affecting the mixing of the first detection mixture and the magnetic beads. The piston mechanism in the second peripheral chamber is driven to rise and fall by the first driving piece 300, the second detection mixed liquid in the central chamber enters the second peripheral chamber and is mixed with the magnetic beads, the magnetic bead powder is dissolved by the second detection mixed liquid to obtain a magnetic bead adhesive substance, and finally the magnetic bead adhesive substance is transferred into the central chamber.

In one embodiment, after the second detection mixture is mixed with the magnetic beads, the second detection mixture can be incubated with the magnetic beads for 1min, 2min, 3min, etc. to bind the analyte (e.g., nucleic acid analyte) in the biological sample to the magnetic beads.

Preferably, in the process of mixing the second detection mixture with the magnetic beads, the piston mechanism inside the second peripheral chamber is driven to repeatedly move up and down, so that the second detection mixture is repeatedly mixed with the magnetic beads.

In order to introduce the waste material resulting from the bead sticker formation process into the second peripheral chamber while leaving the bead sticker in the central chamber, the second driving member 400 is raised and the second driving member 400 is connected to the central member 103 such that the magnetic attracting means 403 of the second driving member 400 is brought close to the central member 103. Because the magnetic equipment 403 can adhere the magnetic bead adherends, the piston mechanism in the second peripheral chamber is lifted, the waste generated in the forming process of the magnetic bead adherends can be led into the second peripheral chamber, and the magnetic bead adherends are adhered to the central chamber by the magnetic equipment 403, so that the separation of the magnetic bead adherends and the waste is realized. After the waste is removed from the interior of the central chamber, the second drive member 400 is lowered, separating the second drive member 400 from the central member 103.

The step S30 further includes the steps of: step S33, communicating the central chamber with the third peripheral chamber, and performing a first washing of the magnetic bead adherends with a first washing solution in the third peripheral chamber;

step S34 is to communicate the central chamber with the fourth peripheral chamber and wash the magnetic bead deposits with the second washing solution in the fourth peripheral chamber for a second time.

Specifically, step S33 and step S34 are provided after step S32, respectively. In steps S33 and S34, the bead adherents are washed with an elution buffer so as to remove the beads, respectively, and the second driving member 400 is maintained in a raised state or a lowered state during the washing, so that the washing buffer can be circulated between the corresponding peripheral chamber 102 and the central chamber by the piston mechanism. After the washing is completed, in order to leave the adhered magnetic beads in the central chamber, the second driving member 400 is maintained in a raised state before the liquid is moved to the third peripheral chamber or the fourth peripheral chamber for the last time in steps S33 and S34, so that the magnetic beads are adhered in the central chamber by the magnetic attraction apparatus 403 in the second driving member 400, and the waste generated by washing the adhered magnetic beads remains in the third peripheral chamber or the fourth peripheral chamber in steps S33 and S34.

Step S30 further includes step S35 of communicating the central chamber with the fifth peripheral chamber, and mixing the elution buffer in the fifth peripheral chamber with the magnetic bead sticker to elute the analyte molecules from the magnetic beads in the magnetic bead sticker to obtain a first detection mixture containing the analyte molecules. Specifically, step S35 is provided after step S34. The main objective of this step is to remove the magnetic beads from the analyte molecules, as follows: the hub 103 is rotated by the second drive member 400 to a position in communication with the fifth peripheral chamber and then the elution buffer inside the fifth peripheral chamber is introduced into the central chamber by the piston mechanism to adhere to the magnetic beads to elute the analyte molecules from the magnetic beads.

To facilitate elution of analyte molecules from the beads, an incubation step is provided after the bead agglutinates are mixed, and the incubation time may be 1min, 2min or 3 min.

Step S30 further includes step S36 of transferring the first detection mixture into the reaction chamber by the piston mechanism inside the sixth peripheral chamber, reacting the first detection mixture with the reagent inside the reaction chamber to obtain an analyte sample, and detecting the analyte sample inside the reaction chamber through the detection window 110 by using the detector. Specifically, one or more reaction reagents are placed in the reaction chamber in advance, and after the first detection mixed solution enters the reaction chamber, the first detection mixed solution and the reaction reagents in the reaction chamber perform Polymerase Chain Reaction (PCR) to obtain an analyte sample. The polymerase chain reaction is characterized in that DNA is denatured into a single strand at a high temperature of 95 ℃ in vitro, a primer and the single strand are combined according to the principle of base complementary pairing at a low temperature (usually about 60 ℃), the temperature is adjusted to the optimal reaction temperature (about 72 ℃) of the DNA polymerase, and the DNA polymerase synthesizes a complementary strand along the direction from phosphate to pentose (5 '-3'). The primers have different colors, each color representing a specific target amplicon, and are capable of targeting the primer in an analyte molecule for detection in a polymerase chain reaction. In particular, the detector may be a fluorometer, with which the analyte sample is detected at the time of the particular detection.

It will be appreciated that after elution of the analyte molecules from the magnetic beads, the magnetic beads can be attracted in the central chamber by the magnetic attracting means 403 of the second drive member 400, and thus the first detection mixture containing the analyte molecules in the central chamber can be directed to the two reaction chambers by the piston mechanism inside the sixth peripheral chamber. Specifically, the second driving member 400 is used to drive the central member 103 to rotate to a position where the sixth peripheral chamber is communicated with the central chamber, the piston mechanism in the sixth peripheral chamber is lowered to expel the gas in the sixth peripheral chamber, then the piston mechanism is lifted to introduce a certain amount of the first detection mixture into the two reaction chambers, then the second driving member 400 is used to rotate the central member 103 relative to the chamber body 106 to block all the passages between the peripheral chamber 102 and the central chamber, and the analyte sample to be detected is obtained in the reaction chambers after the enzyme chain reaction (PCR reaction) is completed.

In step S36, the reaction chamber is heated to a second set temperature T2 by the heating mechanism and is maintained for a second duration S2. In the present embodiment, the second set temperature T2 is about 90 ℃. During the specific operation, the bin body 106 is rotated to contact the heat conductor 122 inside the reaction bin with the heating device 500 which can heat the heat conductor 122 to about 90 ℃, so that the temperature inside the reaction bin reaches 90 ℃, and after the second time period of heat preservation S2, the bin body 106 is rotated again to remove the heating device 500 from contact with the heat conductor 122, so that the temperature inside the reaction bin is reduced to 60 ℃. More specifically, the heating device 500 corresponding to the reaction chamber may be moved several times to vary the temperature inside the reaction chamber from 60 to 90 ℃.

Optionally, after the detection window 110 has completed the detection of the analyte sample by the detector, the guard door 600 on the instant analysis device is triggered to open, so that the reaction chamber 100 will be opened, facilitating the recovery of the reaction chamber 100. Alternatively, after the detection window 110 has completed the detection of the analyte sample by the detector, the user is prompted by a buzzer to manually open the protective door 600 of the instant analysis device to recover the reaction chamber 100.

A "biological sample" in the present specification may comprise any sample obtained from a living body or a viral (or prion) source or other biomolecule source, and comprises any cell type or tissue of a subject from which nucleic acids, proteins and/or other biomolecules may be obtained. The biological sample may be a sample obtained directly from a biological source or a processed sample. For example, the amplified isolated nucleic acids constitute a biological sample. Biological samples include, but are not limited to, bodily fluids such as blood, plasma, serum, cerebrospinal fluid, synovial fluid, urine and sweat, tissue and organ samples from animals and plants, and processed samples derived therefrom.

An "analyte" in the present specification refers to a substance, compound or component whose presence or absence in a sample must be detected.

By "reaction mixture" in this specification is meant a solution containing some or all of the necessary reactants to perform a reaction, which may include, but is not limited to, buffers, salts, cofactors, scavengers, and the like, to maintain the pH at a selected level during the reaction.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be appropriately combined to form other embodiments as will be appreciated by those skilled in the art.

The technical principle of the present invention is described above in connection with specific embodiments. The description is made for the purpose of illustrating the principles of the utility model and should not be construed in any way as limiting the scope of the utility model. Based on the explanations herein, those skilled in the art will be able to conceive of other embodiments of the present invention without inventive effort, which would fall within the scope of the present invention.

Claims

1. A kit, comprising:

2. The kit of claim 1, wherein the reaction chamber and/or the sample container is provided with an identification code for identification.

3. A device for real-time analysis, comprising a device body, wherein the device body is provided with the reagent cartridge of claim 1 or 2, and further provided with a first driving member and a second driving member, the first driving member is used for driving the piston mechanism of the reagent cartridge to move, and the second driving member is used for driving the central member of the reagent cartridge to rotate.

4. The instant analysis device according to claim 3, wherein the first driving member comprises a first rotating motor and a rack, an output shaft of the first rotating motor is connected with the rack, the output shaft of the first rotating motor can drive the rack to move in the first direction, and a connecting portion which is connected with the connecting groove of the piston mechanism in a matching manner is arranged at the lower end of the rack.

5. The instant analysis device according to claim 3, wherein the second driving member comprises a rotating spline, a magnetic attraction device, and a second rotating motor, the rotating spline has a third protrusion, the lower end of the central member is provided with a third recess, the third protrusion is connected with the third recess in a matching manner, the second rotating motor is located on a side of the rotating spline away from the central member, an output shaft of the second rotating motor passes through the magnetic attraction device and is connected with the rotating spline, and the magnetic attraction device can attract the magnetic beads inside the central chamber.

6. The real-time analysis device according to claim 3, wherein a heating device is further disposed on the device body for connecting with the heating mechanism, and the heating device provides a heat source for the heating mechanism.

7. The device of claim 6, wherein the heating apparatus comprises an apparatus body, and the apparatus body is provided with a heat conductor interface and a heat sensor, and the heat conductor interface is selectively connected to the heat conductor.

8. The point-of-care assay device of any one of claims 3 to 7, further comprising a detector capable of emitting an optical signal to detect the analyte sample in the reaction chamber at a detection window and a scanner for scanning an identification code on the cartridge.

9. The point-of-care assay device of claim 8, further comprising a printer for printing the results of the detection of the analyte sample by the detector.