CN212355036U - Kit - Google Patents

Abstract

The utility model discloses a kit, which comprises a box body and a box cover connected with the box body, wherein the box body is sequentially provided with a standard product storage area, a reagent storage area and a configuration product storage area; the standard product storage area is provided with a plurality of first storage positions for storing quantitative standard products; the standard product storage area is provided with a cooling device; the reagent storage area and the configuration product storage area are respectively provided with a second storage position and a third storage position which are different in size; and the inner bottom surface of the box cover is adhered with a bar code for providing article information of the standard article storage area, the reagent storage area and the configuration article storage area. With this structural design for this kit design is comparatively reasonable, and convenient operation, easily preserves.

Description

Kit

Technical Field

The utility model relates to the field of biotechnology, especially, relate to a kit.

Background

The kits available on the market for DNA quantification by fluorescence quantification devices are various, and in general, the kits integrate four components, namely, quantification buffer, fluorescent dye, standard S1 and standard S2, into a simple paper box. Before unpacking, manufacturers generally recommend that the whole kit be stored in a refrigerator at 4 ℃; after the seal is removed, the standard substance S1 and the standard substance S2 are stored in a refrigerator at 4 ℃, and the quantitative buffer solution and the fluorescent dye are stored at room temperature together with a paper box.

The quantitative operation is generally simpler: according to the sample size, according to 199: 1, adding quantitative buffer solution and fluorescent dye into a 2ml or 10ml centrifuge tube to form quantitative reaction liquid, and then respectively mixing the quantitative reaction liquid and the fluorescent dye according to the ratio of 190: adding the quantitative reaction liquid, the standard substance S1 and the standard substance S2 into a 0.5ml esin quantitative tube in a proportion of 10, uniformly mixing, and reacting for 2min in a dark place to perform a calibration reaction; the samples are then scaled according to 199: 1 proportion in 0.5ml Aisi quantitative tube, need mix well and keep out of the sun for 2min before detecting, this process generally needs laboratory to prepare adaptation 2ml, 10ml and 0.5ml centrifuge tube shelf by oneself.

The above-mentioned kits generally have several problems: 1. different storage conditions are needed before and after unsealing, and reagents need to be taken out and taken back during operation; 2. the buffer solution and the fluorescent dye after the unsealing are stored without fixed positions, and the reagent is very easy to overturn when in use; 3. the centrifuge tube rack provided by a laboratory does not meet the light-shielding condition generally, and the quantitative reaction liquid is prepared and split charged and then decomposed by light, so that the quantification is inaccurate; 4. the quantitative reaction tube is stored on a non-special centrifuge tube rack, so that the tube wall is easily polluted, and the quantitative accuracy is influenced.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a kit, this kit reasonable in design, convenient operation, easily save.

To achieve the purpose, the utility model adopts the following technical proposal:

a kit comprises a box body and a box cover connected with the box body, wherein the box body is sequentially provided with a standard product storage area, a reagent storage area and a configuration product storage area; the standard product storage area is provided with a plurality of first storage positions for storing quantitative standard products; the standard product storage area is provided with a cooling device; the reagent storage area and the configuration product storage area are respectively provided with a second storage position and a third storage position which are different in size; and the inner bottom surface of the box cover is adhered with a bar code for providing article information of the standard article storage area, the reagent storage area and the configuration article storage area.

The standard product storage area is provided with a paper box or a plastic box, and the top cover of the paper box or the plastic box is provided with two first round holes for forming the first storage position.

Wherein, the cooling device is arranged into a biological ice bag stored in the cavity of the paper box or the plastic box.

The reagent storage area is provided with a dark sponge liner, and the sponge liner is provided with a plurality of second round holes with different apertures for forming the second storage positions.

The configuration product storage area is provided with a test tube rack convenient to take and place, and the test tube rack is provided with a plurality of third round holes with different apertures for forming a third storage position.

Wherein, the test-tube rack is followed box body width direction is provided with the multirow the third round hole.

Wherein, a magnet which is magnetically connected with the box cover is embedded in one side wall of the box body.

The utility model has the advantages that: the utility model provides a kit, which comprises a box body and a box cover connected with the box body, wherein the box body is sequentially provided with a standard product storage area, a reagent storage area and a configuration product storage area; the standard product storage area is provided with a plurality of first storage positions for storing quantitative standard products; the standard product storage area is provided with a cooling device; the reagent storage area and the configuration product storage area are respectively provided with a second storage position and a third storage position which are different in size; and the inner bottom surface of the box cover is adhered with a bar code for providing article information of the standard article storage area, the reagent storage area and the configuration article storage area. With this structural design for this kit design is comparatively reasonable, and convenient operation, easily preserves.

Drawings

Fig. 1 is a schematic structural diagram of a kit according to the present invention.

Detailed Description

The technical solution of the present invention is further explained by the following embodiments with reference to the accompanying drawings.

Referring to fig. 1, the present embodiment provides a kit, including a box body 1 and a box cover 2 connected to the box body 1, where the box body 1 is sequentially provided with a standard product storage area 3, a reagent storage area 4, and a configuration product storage area 5; the standard product storage area 3 is provided with a plurality of first storage positions for storing quantitative standard products; the standard product storage area is provided with a cooling device; the reagent storage area 4 and the configuration product storage area 5 are respectively provided with a second storage position and a third storage position which are different in size; the inner bottom surface of the box cover 2 is adhered with a bar code for providing the article information of the standard article storage area 3, the reagent storage area 4 and the configuration article storage area 5. When the kit is used, the bar codes are scanned to obtain the information of the production date and the batch number of the kit, and the PDF version instruction and the online support of the kit can be obtained, and the paper version instruction is not provided for the kit independently.

Further specifically, the standard storage area 3 in this embodiment is provided with a paper box or a plastic box, two first circular holes for forming the first storage position are formed in a top cover of the paper box or the plastic box, and the cooling device is a biological ice bag stored in a cavity of the paper box or the plastic box. The arrangement of the biological ice bag can ensure that the standard substance can be in a low-temperature environment for a long time when the kit is used, and the standard substance is prevented from degrading to influence calibration;

furthermore, in this embodiment, the reagent storage area 4 is provided with a dark-colored sponge pad, and the sponge pad is provided with a plurality of second round holes with different apertures for forming the second storage position; one opening is suitable for placing a 50ml centrifuge tube, the centrifuge tube is used for containing self-prepared quantitative buffer solution, and the other opening is suitable for placing a 2ml centrifuge tube and is used for storing finished fluorescent dye. Meeting the light-resistant condition required by dye storage.

Further, the configuration article storage area 5 is provided with a test tube rack convenient for taking and placing, the test tube rack is provided with a plurality of third round holes with different apertures for forming the third storage position, the test tube rack is provided with a plurality of rows of the third round holes along the width direction of the box body 1, and more specifically, the configuration storage area is a strip-shaped box provided with a plurality of openings with different sizes for the kit. When in use, the area can be taken out and placed on a working table for independent use, and can also be directly operated on the working table. Wherein 2 trompils are established altogether to left first row, are applicable to respectively and deposit 2ml and 10ml centrifuging tube for correspond placing of different centrifuging tubes when preparing quantitative reaction liquid, satisfy the light-resistant requirement. The right side 3 rows are 24 small openings in total, are suitable for placing 0.5ml love into a quantitative centrifuge tube, are special quantitative pipe racks, avoid dirt, and can meet the light-shading requirement.

In addition, in order to fix the box cover 2 and the box body 1 conveniently, a magnet magnetically connected with the box cover 2 is embedded in one side wall of the box body 1 in the embodiment.

When the kit is used for testing, the kit can be taken out from a refrigerator at 4 ℃ in advance, a newly unsealed sample is uncovered, and then a bar code in the box cover 2 is scanned to obtain kit information and an operation instruction.

Then, according to the number of samples to be quantified, the total volume of the quantitative reaction liquid is calculated: the number of samples is the number of samples to be measured, the number of standard products 2 and the number of sample adding loss 1;

total volume (199 μ L quantitation buffer +1 μ L fluorescent dye) total number of samples

Then taking a 2mL or 10mL reaction solution preparation tube according to the total volume, placing the reaction solution preparation tube on a corresponding tube frame of a kit preparation storage area 5, then preparing a 0.5mL quantitative tube according to the number of samples, subpackaging 190 mu L of quantitative reaction solution in S1 and S2 tubes, subpackaging 199 mu L of quantitative reaction solution in a sample tube, then adding 10 mu L of standard substance S1 in an S1 tube, adding 10 mu L of standard substance S2 in an S2 tube, adding 1 mu L of sample in the sample tube, sealing a cover, carrying out vortex oscillation for 3-5S, placing the reaction solution in a corresponding tube frame of the kit preparation storage area 5, and incubating for 2min in a dark place.

Standards S1 and S2 were inserted for calibration according to the fluorescent quantitation device calibration procedure, respectively, and then sample tubes were inserted in sequence to quantitate the samples.

The technical principle of the present invention is described above with reference to specific embodiments. The description is made for the purpose of illustrating the principles of the invention and should not be construed in any way as limiting the scope of the invention. Based on the explanations herein, those skilled in the art will be able to conceive of other embodiments of the present invention without any inventive effort, which would fall within the scope of the present invention.

Claims (7)

1. A kit, characterized in that: the box body is sequentially provided with a standard product storage area, a reagent storage area and a configuration product storage area; the standard product storage area is provided with a plurality of first storage positions for storing quantitative standard products; the standard product storage area is provided with a cooling device; the reagent storage area and the configuration product storage area are respectively provided with a second storage position and a third storage position which are different in size; and the inner bottom surface of the box cover is adhered with a bar code for providing article information of the standard article storage area, the reagent storage area and the configuration article storage area.

2. A kit according to claim 1, wherein: the standard product storage area is provided with a paper box or a plastic box, and two first round holes used for forming the first storage positions are formed in the top cover of the paper box or the plastic box.

3. A kit according to claim 2, wherein: the cooling device is arranged into a biological ice bag stored in the cavity of the paper box or the plastic box.

4. A kit according to claim 2, wherein: the reagent storage area is provided with a dark sponge pad, and the sponge pad is provided with a plurality of second round holes with different apertures for forming the second storage positions.

5. A kit according to claim 2, wherein: the configuration article storage area is provided with a test tube rack convenient to take and place, and the test tube rack is provided with a plurality of third round holes with different apertures for forming a third storage position.

6. A kit according to claim 5, wherein: the test-tube rack is provided with the multirow along box body width direction the third round hole.

7. A kit according to claim 1, wherein: and a magnet magnetically connected with the box cover is embedded in one side wall of the box body.

Images