CN115703990A

CN115703990A - Micro amplification instrument, reactor and pocket type quick detection equipment

Info

Publication number: CN115703990A
Application number: CN202110968703.3A
Authority: CN
Inventors: 岂源
Original assignee: Beijing Qingfengtang Pharmaceutical Technology Co ltd
Current assignee: Beijing Qingfengtang Pharmaceutical Technology Co ltd
Priority date: 2021-07-30
Filing date: 2021-08-23
Publication date: 2023-02-17
Also published as: CN115704049A; CN215906211U; CN215667945U; CN115703989A; CN215757272U; CN115703991A; CN215667988U; CN115786097A; CN215906212U

Abstract

The invention discloses a micro amplification instrument which comprises a bearing part, a heating part, an installation frame body and a shell, wherein the bearing part is provided with a bearing cavity position for placing a sample to be detected; the heating part is arranged around the periphery of the bearing cavity position for electric heating. The invention also discloses a reactor, which comprises a sample adding part, a sample part and a reaction part which are sequentially connected, wherein the sample adding part is movably connected with the sample part, the sample adding part is of a piston structure, a sample preserving fluid is pre-filled in the sample part, and a reaction system is filled in the reaction part; the connecting part of the reaction part and the sample part is provided with micropores, and the pore diameter of the micropores is not more than the capillary length of the sample liquid or the sample preservation liquid. The invention also discloses pocket type quick detection equipment which comprises the miniature amplification instrument and a reactor, wherein the reactor is matched with the amplification instrument for use, and the reactor is placed in the bearing cavity position. The micro amplification instrument, the reactor and the pocket type quick detection equipment can be used in any occasions, do not need to be operated by professionals, and have clear and easily-judged results.

Description

Micro amplification instrument, reactor and pocket type quick detection equipment

Technical Field

The invention relates to the technical field of nucleic acid detection (DNA or RNA), in particular to a micro-amplification instrument, a reactor and a pocket type quick detection device.

Background

Nucleic acid detection, as a method having high sensitivity and specificity, has been widely used in many fields such as disease diagnosis, food safety, infectious disease control, and the like. Detection of specific nucleic acid sequences in a simple manner can confer greater value in point-of-care (point-of-care) diagnostics and in point-of-care pathogen detection.

PCR (polymerase chain reaction) is a molecular biology technique for amplifying and amplifying a specific DNA fragment, which can be regarded as special DNA replication in vitro, and the biggest characteristic of PCR is that a trace amount of DNA can be greatly increased. However, PCR, a classical nucleic acid detection method, has inherent denaturation-renaturation-extension cycles, which require that a thermocycler device be necessary as a support, and a professional laboratory also be a necessary condition because of aerosol contamination problems. Among them, the PCR extension technology platform, particularly the quantitative PCR (qPCR) method, is the most widely used pathogen detection method and is considered as a new gold standard test. qPCR provides a much shorter sample-to-result time (3 to 5 hours). However, although qPCR is widely accepted, it is limited by relying on standard reference substances (standard curves) for quantification. Unreliable and inconsistent commercial standard reference materials may also affect the accuracy of qPCR quantification. In addition, qPCR is susceptible to inhibition by naturally occurring substances in environmental samples (e.g., heavy metals and organic matter), leading to inaccurate or false negative results in target quantification. Therefore, the application of PCR in the fields of point-of-care rapid diagnosis (POCT), on-site rapid detection and the like is greatly limited. Compared to qPCR, recent digital PCR techniques have proven to be more robust solutions for detection of microbial pathogens in environmental samples. Digital PCR is based on partitioning (partioning) and poisson statistics, so there is no need to compare external quantification standards to quantify samples of unknown concentration. However, implementing digital PCR methods for use with point-of-use applications (point-of-use applications) can be challenging. This is because digital PCR requires expensive instrumentation (i.e., bio-rad droplet digital PCR), a fully equipped laboratory environment, and trained technicians to perform the assays. These factors severely limit the accessibility and applications of digital PCR in resource-limited contexts.

To overcome these disadvantages, a large class of new methods for isothermal nucleic acid amplification has emerged, with LAMP being the most interesting and promising method.

Loop-mediated isothermal amplification (LAMP) is an alternative PCR nucleic acid amplification method developed by Rongy chemical Co., ltd. Japan in 2000. It is characterized by that 4 specific primers are designed according to 6 regions of target gene, under the action of strand displacement DNA polymerase (Bst DNA polymerase), the amplification is implemented at constant temp. of 60-65 deg.C for about 15-60 min ⁹ ～10 ¹⁰ The nucleic acid amplification is simple to operate, strong in specificity, easy to detect products and the like. LAMP, as a molecular biology detection technology, has the characteristics of high specificity, high sensitivity, simplicity, convenience and low cost, and is widely applied to diagnosis of clinical diseases, qualitative and quantitative detection of epidemic bacteria or viruses, sex identification of animal embryos and gene chips.

Thus, LAMP detection is a rapid, simplified, low cost assay for detecting microorganisms to provide molecular assays outside of a centralized laboratory, for example, where on-site point-of-use testing of environmental water in resource-limited locations is required.

LAMP detection is performed under isothermal conditions, which can be maintained in different instruments, such as a thermocycler and a water bath. The apparatus enables amplification of the DNA/cDNA of a sample by heating the detection chamber inside the device to detect pathogens.

The micro-amplification instrument performs amplification reaction by using strand displacement type DNA polymerase under the constant temperature condition, can realize 109-1010 times of amplification within 15-60 minutes, can generate a large amount of amplification products, namely magnesium pyrophosphate white precipitate, and can judge whether a target gene exists by observing the existence of the white precipitate by naked eyes. The LAMP method has the advantages of high specificity and high sensitivity, is very simple to operate, has low requirements on instruments in the application stage, can realize reaction by using a simple constant temperature device, is very simple in result detection, can directly observe white precipitates or green fluorescence by naked eyes, does not need to carry out gel electrophoresis observation results unlike the common PCR method, and is a method suitable for rapid detection on site and in basic level.

The isothermal amplification instrument can be applied to the fields of pathogenic microorganisms, species identification, animal epidemic diseases, transgenosis and the like, but the traditional isothermal amplification instrument is large in size and inconvenient to carry, so that the isothermal amplification instrument can only be used in a laboratory.

Because of the limitations of nucleic acid detection reagents and detection equipment based on amplification, the problem of extraction of nucleic acid or other samples to be detected cannot be solved by amplification operation in the existing detection, multiple uncovering is also needed in the amplification process, especially when an eight-connected tube is used as a reactor, and operation in a professional PCR laboratory is also needed to avoid pollution, so that the nucleic acid detection in the prior art cannot realize field sampling and field detection, especially a reactor which can directly complete reaction once after a sample is directly added is not available, and the traditional eight-connected tube or EP tube (centrifugal tube) is still adopted, which is an important elbow stopper that the nucleic acid detection cannot be well applied to POCT and the development and application of pathogenic microorganisms.

Disclosure of Invention

The technical problem to be solved by the invention is as follows: aiming at the technical problems in the prior art, the invention provides a micro-amplification instrument, a reactor and a pocket type quick detection device, which can realize the whole nucleic acid detection without a PCR laboratory and aerosol pollution without opening a cover after directly adding a sample to be detected.

In order to solve the technical problems, the technical scheme provided by the invention is as follows:

a micro amplification instrument comprises a bearing part, a heating part, a mounting frame body and a shell, wherein the bearing part and the heating part are arranged in the shell through the mounting frame body; the bearing part is provided with a bearing cavity position for placing a sample to be tested; the heating part is arranged around the periphery of the bearing cavity position for electric heating, and the micro-amplification instrument further comprises a centrifugal part which drives the bearing part to rotate and do centrifugal motion.

As a further improvement of the above technical solution:

preferably, in the above technical solution, the bearing portion is provided with a bearing cylinder, and an inner cavity of the bearing cylinder forms a bearing cavity.

In the above technical solution, preferably, the heating portion includes a plurality of heating sheets, and the heating sheets are surrounded around the carrying cylinder, so that the carrying cylinder can be uniformly heated.

Preferably among the above-mentioned technical scheme, the miniature amplification appearance still includes centrifugal part and charging power supply unit, centrifugal part is connected through a drive belt with the bearing cylinder, charging power supply unit is connected the energy supply with centrifugal part electricity, it is rotatory to drive the bearing cylinder from the centrifugal part, be equipped with the bearing between bearing cylinder and the installation support body. The centrifugal part and the bearing part are arranged side by side, the motor of the centrifugal part is small and exquisite, and the bearing cylinder can be driven to rotate centrifugally, so that the whole equipment is simple, small and convenient to carry.

Preferably, in the above technical scheme, the housing is provided with an equipment port for placing a sample to be tested, and the equipment port is communicated with the bearing cavity. That is, the whole amplification instrument has only one inlet, is sealed in the shell, is not easy to enter dust and has long service life.

Preferably, among the above-mentioned technical scheme, the bearing cylinder is a hollow cylinder or the bottom is transparent material, the amplification instrument still includes the portion of shooing, the portion of shooing is located the bottom of bearing cylinder. The photographing part adopts a small-sized high-precision camera and is provided with a flash lamp, such as a camera of a mobile phone.

The technical scheme of the invention also provides a reactor, wherein the sample to be detected is placed in the reactor; the reactor comprises a sample adding part, a sample part and a reaction part which are sequentially connected, wherein the sample adding part is movably connected with the sample part, the sample adding part is of a piston structure, a sample preserving fluid is pre-filled in the sample part, and a reaction system is filled in the reaction part; the connecting part of the reaction part and the sample part is provided with micropores, and the aperture of each micropore is not more than the capillary length of the sample liquid or the sample preservation liquid; under the sealed state of the reactor, the sample adding part moves towards the reaction part by external force to realize that the sample liquid passes through the micropores and is pressed into the reaction part under the sealed state.

As a further improvement of the above technical solution:

in the above technical solution, preferably, at least one reaction chamber is arranged in the reaction part, an independent reaction system is preset in the reaction chamber, the micropores are arranged at the orifice of the reaction chamber, and each reaction chamber corresponds to one micropore.

Preferably among the above-mentioned technical scheme, the reaction portion is equipped with the reposition of redundant personnel stopper, the reposition of redundant personnel stopper adopts flexible material to make, the reposition of redundant personnel stopper is located the junction of reaction portion and sample portion, the reaction chamber is independently sealed through the reposition of redundant personnel stopper, the reposition of redundant personnel stopper is located to the micropore.

In the above technical solution, preferably, the pore size of the micropores is 0.3 to 0.6mm.

Preferably, in the above technical solution, the port of the reaction chamber near the sample part is closed, each reaction chamber is in an independent sealing state before reaction, and the port is closed by a sealing plug or a sealing film. Because a sealing film or a sealing plug is provided, the reaction chamber can be kept in a sealed state even in the case where the threaded joint of the reaction part and the sample part is unscrewed.

Preferably among the above-mentioned technical scheme, the reaction portion is equipped with interior reaction tube and the outer tube that cup joints, interior reaction tube chucking is in the outer tube, the outer tube is equipped with the screw thread with sample portion coordination spiro union. The reaction part is arranged into an inner pipe and an outer pipe so as to facilitate the processing of the reaction cavity during production.

Preferably among the above-mentioned technical scheme, sample addition portion is including the spill stopper and the application of sample cap that cup joint, install one in the application of sample cap and insert the spill stopper and be the rigid protruding piece that cup joints, application of sample cap still is equipped with the threaded connection section with sample site coordination spiro union, can realize the sealing connection between application of sample portion and the sample site, accessible unidirectional rotation application of sample portion realizes sample liquid to the sample site pressurization again and gets into in the reaction portion.

Preferably, in the above technical solution, the sample part is a coaxial sleeve, wherein the inner tube is a hollow tube, the inner diameter of the hollow tube is in sealing fit connection with the concave plug, and one end of the outer tube is provided with a thread which is in coordination threaded connection with the sample adding part; when the reactor is sealed, one section of the inner tube is positioned in the gap between the concave plug and the sample adding part; the sample preservation solution is pre-arranged in the cavity of the inner tube before reaction. The inner and outer tubes of the sample section may be integral and divided into inner and outer tubes to reduce machining costs.

In the above technical solution, preferably, the sample part further comprises a sealing member disposed between the reaction part and the sample part, the sealing member is used for sealing the sample preservation solution, and even when the screw joint between the reaction part and the sample part is unscrewed, the inner cavity of the sample part can be in a sealed state.

A second embodiment of the reactor of the present application is: the application of sample portion includes application of sample stopper and application of sample lid, application of sample lid is equipped with the inner chamber, application of sample stopper is located application of sample lid's inner chamber, just application of sample stopper has resistance unidirectional motion in the inner chamber, application of sample stopper constitutes piston structure with application of sample lid's inner chamber, and under the reactor encapsulated situation, through application of sample stopper to sample portion direction motion messenger's sample liquid gets into the reaction chamber.

In the above technical solution, preferably, the reaction portion and the sample portion are integrally formed, and the reaction portion and the sample portion are separated by a shunt plug.

The technical scheme of the invention also provides pocket type quick detection equipment, which comprises the micro amplification instrument and the reactor, wherein the reactor and the amplification instrument are matched for use, and the reactor is placed in a bearing cavity.

Compared with the prior art, the micro amplification instrument, the reactor and the pocket type quick detection equipment provided by the invention have the following advantages:

(1) The micro amplification instrument adopts the rechargeable lithium battery, has the whole length and width of about 7 x 2 x 6cm, can be put in a pocket or carried in a bag, does not need to be electrified, can be used in any occasions, does not need to be operated by professionals, has clear and easy judgment on the result, is particularly suitable for being used at home or on a sampling and inspection site, and can obtain the result 15 minutes after the sample is taken.

(2) According to the micro amplification instrument, multiple common detections can be simultaneously carried out on the same sample by the amplification instrument; heating the reaction by a heating part in the reaction process to maintain a constant temperature environment required by the amplification reaction; and vibrating and mixing the sample liquid and the reaction system by centrifugal rotation.

(3) The pocket type rapid detection equipment can pre-add a reaction system in the amplification operation, and the pre-added reaction system not only avoids the limitation of a field configuration reaction system on the environment, simplifies the system configuration steps before detection, but also can ensure the rapid implementation and the simple and convenient use of the detection. The subsequent amplification operation only needs to add a sample to be detected, the sample can directly react after reaching the amplification reaction condition (such as temperature) without adding liquid again, so that the reactor only needs to open the cover once in the detection process to add the sample to be detected, the sample is not in contact with other components in the reaction system, the sample is fully contacted and directly reacts in the reaction process, the cover does not need to be opened again, the whole nucleic acid detection process can be realized, the detection condition is basically unlimited, no aerosol pollution exists, and the result can be obtained by processing the reactor after the detection reaction is finished.

(4) The pocket type rapid detection equipment can be realized by matching simple heating equipment (even a vacuum cup) with the reactor of the invention aiming at public health events, does not need to be operated by professionals, has clear and easily-judged results, is suitable for the requirements of various medical detection scenes at home and abroad at present, and can greatly improve the molecular diagnosis capability of the pocket type rapid detection equipment especially in relatively laggard areas.

Drawings

FIG. 1 is a schematic view of an embodiment of the present invention in use (housing not shown).

FIG. 2 is a schematic diagram of an exploded structure of the micro-amplifier of the present invention.

FIG. 3 is a schematic view of the structure of example 1 of the reactor of the present invention.

FIG. 4 is a schematic diagram of the structure of example 2 of the reactor of the present invention.

The reference numbers in the figures illustrate:

1. a bearing part; 11. a load bearing cavity; 12. a carrying cylinder; 2. a heating section; 3. a centrifuge portion; 4. a motor; 5. a photographing part; 6. a housing; 61. a cover; 7. installing a frame body; 71. an upper mounting seat; 72. a base; 8. a reactor; 81. a sample section; 811. an outer tube; 812. an inner tube; 813. a seal member; 82. a sample addition part; 821. a concave plug; 822. a sample adding cap; 823. tearing the pull ring; 824. a sample adding plug; 825. a sample application cover; 83. a reaction section; 831. an outer sleeve; 832. an inner reaction tube; 833. a reaction chamber; 834. a shunt plug; 8341. a boss member; 8342. and (4) micro-pores.

Detailed Description

The present invention will be described more fully hereinafter with reference to the following examples. The following examples are illustrative only and are not to be construed as limiting the invention.

The experimental procedures in the following examples are conventional unless otherwise specified. The experimental materials used in the following examples were all commercially available unless otherwise specified.

Example 1

Fig. 1 to 3 show an embodiment of a pocket-type rapid test apparatus according to the present invention, the apparatus includes a micro-amplifier, the micro-amplifier includes a support part 1, a heating part 2, a mounting frame body 71 and a housing 6, the support part 1 and the heating part 2 are disposed in the housing 6 through the mounting frame body 71; the bearing part 1 is provided with a bearing cavity 11 for placing a sample to be tested; the heating part 2 is positioned below the bearing part 1 to electrically heat the bearing part 1, and the device also comprises a rechargeable battery.

In this embodiment, the mounting frame body 71 includes an upper mounting seat 71 and a base 72, the carrying portion 1 is provided with a carrying cylinder 12, and the carrying cylinder 12 is mounted on the upper mounting seat 71. The inner cavity of the carrier cylinder 12 forms a carrier cavity 11. The heating part 2 includes a plurality of heating sheets, which are arranged around the circumference of the carrier cylinder 12, and ceramic heating sheets may be used.

In this embodiment, the micro-amplifier further includes a centrifugal part 3, the centrifugal part 3 is connected to the carrying cylinder 12 through a transmission belt, the centrifugal part 3 includes a motor 4 and a transmission shaft, the motor 4 is installed between the upper mounting seat 71 and the base 72, the transmission shaft extends out of the upper mounting seat 71, and the transmission belt is in meshing transmission with a gear on the transmission shaft. The bearing cylinder 12 is provided with a belt groove matched with the transmission belt, the motor 4 drives the transmission shaft, and the bearing cylinder 12 is rotated through the transmission belt. A bearing is arranged between the bearing cylinder 12 and the mounting frame body 71.

In this embodiment, the housing 6 is provided with an equipment port for placing a sample to be tested, and the equipment port is communicated with the bearing cavity 11. The volume of the amplification instrument is reduced, so that the amplification instrument is smaller.

In this embodiment, the carrying cylinder 12 is a hollow cylinder or the bottom is made of transparent material, the amplification apparatus further includes a photographing part 5, and the photographing part 5 is located at the bottom of the carrying cylinder 12.

The amplification instrument of the embodiment is also provided with a control module for controlling the operation of each part. The control module comprises a temperature sensor, a processing unit and a wireless unit. The temperature sensor is used for detecting the temperature of the bearing part 1 and transmitting the detection data to the processing unit. The camera is electrically connected with the processing unit. The wireless unit adopts a wireless network or a Bluetooth form, and transmits the photographing result of the processing unit to the host or the mobile phone, the technology of data transmission through the wireless unit in the prior art is very mature, and no more explanation is made here, and any structure capable of realizing the technology and being small can be used in the embodiment. The whole length, width and height of the micro-amplification instrument are about 7 x 2 x 6cm, the micro-amplification instrument can be put into a pocket or carried in a bag, the power-on is not needed, the micro-amplification instrument can be used in any occasions, the operation of professionals is not needed, the result is clear and easy to judge, the micro-amplification instrument is particularly suitable for being used at home or in a sampling and detecting field, and the result can be obtained 15 minutes after a sample is taken.

The device of the invention is also provided with a reactor 8 used in cooperation with a micro-amplification instrument, the reactor 8 comprises an adding part 82, a sample part 81 and a reaction part 83 which are connected in sequence, wherein the adding part 82 and the sample part 81 are movably connected to realize a sealing state, the adding part 82 is of a piston structure, a sample preserving fluid is pre-filled in the sample part 81, and a reaction system is arranged in the reaction part 83; a micropore 8342 is arranged at the joint of the reaction part 83 and the sample part 81, and the pore diameter of the micropore 8342 is not more than the capillary length of the sample liquid or the sample preservation liquid; the surface tension of the liquid at the micropores is greater than its gravity. In this embodiment, the pore diameter of the micropores is 0.3 to 0.6mm. In the sealed state of the reaction vessel 8, the sample addition part 82 moves toward the reaction part 83 by an external force, and the sample liquid is pressed into the reaction part 83 through the micropores 8342 in the sealed state. In order to be conveniently placed in the micro-amplification instrument, the whole reactor 8 adopts a circular tube type structure, and particularly, the whole body is preferably made of transparent plastic materials.

In this embodiment, a plurality of reaction chambers 833 are provided in the reaction portion 83, independent reaction systems are provided in the reaction chambers 833 in advance, simultaneous detection of a plurality of detection items for the same sample can be realized, and the end portion of each reaction chamber 833 near the junction is provided with a micropore 8342, so that liquid cannot flow into the reaction chamber 833 from the sample portion 81 without an external force.

In this embodiment, the reaction portion 83 is provided with an inner reaction tube 832 and an outer sleeve 831 which are sleeved with each other, the inner reaction tube 832 is clamped in the outer sleeve 831, one end of the outer sleeve 831 is provided with an internal thread which is in coordination threaded connection with the sample portion 81, the reaction portion 83 is provided with a shunt plug 834, the shunt plug 834 is made of a flexible material, and the reaction portion 83 is made of a transparent rubber material. The shunting plug 834 is located at a joint of the reaction cavity 833 and the sample part 81, one section of the upper part of the reaction part 83 is an internal thread section, the position of the reaction cavity 833 is not located at the joint of the internal thread, namely, the shunting plug 834 divides the joint of the internal thread and the reaction cavity 833, one surface of the shunting plug 834 protrudes into the reaction cavity 833 to seal a cavity opening, each protrusion has a micropore 8342, the micropores 8342 penetrate through the protrusion, and one surface of the shunting plug 834, which faces the sample part 81, is a smooth surface. Reaction chamber 833 is sealed independently by a shunt plug 834. Before reaction, a reaction system is pre-buried in each reaction cavity 833, and the reaction system is sealed and buried, for example, a material which is not compatible with water and can change the form along with the change of temperature is used for sealing the reaction system. Each reaction chamber 833 can be provided with a different reaction system for detecting different items. Since the reactor 8 is small and compact as a whole, it is preferable to provide 4 reaction chambers 833.

In this embodiment, the outer sleeve 831 is a hollow tube, the inner reaction tube 832 is clamped in the outer sleeve 831, the inner reaction tube 832 is provided with one or more than one protruding ridge for positioning, the outer sleeve 831 is provided with a corresponding groove, and the inner reaction tube 832 and the outer sleeve 831 can be integrally formed or can be manufactured separately. In practice, because the reactor 8 is small, the outer sleeve 831 and the inner reaction tube 832 are typically manufactured in two parts and then assembled for ease of manufacture and ease of handling for embedding the reaction system.

In this embodiment, at least one rib or groove is formed on the outer surface of the outer sleeve 831, i.e., the outer surface of the reaction portion 83, for positioning the reaction chamber 8 when it is placed in the loading chamber 11 of the micro-amplifier, and the loading chamber 11 is provided with a groove or rib.

In this embodiment, the sample adding part 82 includes a sleeved concave plug 821 and a sleeved sample adding cap 822, one section of the sample adding cap 822 connected with the sample part 81 is provided with an external thread connecting section, a rigid protruding part 8341 inserted into the sleeved concave plug 821 is arranged in the sample adding cap, the protruding part 8341 is cylindrical or cylindrical, the protruding part 8341 and the external thread connecting section are of concentric rings, and an annular cavity is arranged between the protruding part 8341 and the external thread connecting section. The concave plug 821 is made of transparent rubber materials, the concave plug 821 adopts a piston structure with a cylindrical cavity arranged inside, a circular ring extending outwards is arranged at the cavity opening of the top of the piston, the outer diameter of the circular ring is the same as that of the annular cavity, the diameter of the piston head at the other end is slightly larger than that of the inner cavity of the sample part 81, and the function of the piston head in the inner cavity of the sample part 81 is achieved. When the concave plug 821 is sleeved on the sample adding cap 822, the head part of the piston protrudes out of the sample adding cap 822, so that the sealed connection between the sample adding part 82 and the sample part 81 can be realized, and the sample liquid can enter the reaction part 83 by pressurizing the sample adding part 82 to the sample part 81 through the unidirectional rotation.

In this embodiment, the sample portion 81 is a coaxial sleeve, the inner tube 812 and the outer tube 811 are hollow tubes, one end of the outer tube 811 is provided with an internal thread, and the diameter of the section with the external thread is slightly smaller than the diameter of the middle section of the outer tube 811 and the section with the internal thread. The inner tube 812 has the same diameter as the small diameter of the outer tube 811. The sample part 81 is further provided with a sealing member 813, the sample storage solution is sealed in the cavity of the inner tube 812 by the sealing member 813 and the sample addition part 82 before the reaction, and the sealing member 813 is removed when the sample to be measured is put therein.

In this embodiment, the external thread of the sample addition cap 822 and the internal thread of the outer tube 811 are screwed into the cavity between the outer tube 811 and the inner tube 812 of the sample addition part 82, and the concave plug 821 is located in the inner tube 812 and seals the inner tube 812. The internal thread of the outer sleeve 831 of the reaction part 83 is in threaded connection with the external thread of the outer tube 811, and the end of the inner tube 812 at the connection extends outwards to form a limiting ring, so as to limit the position of the inner tube 812 in the outer tube 811 and prevent the sample preserving fluid or the sample fluid from entering the outer tube 811 into the compartment of the inner tube 812.

In this embodiment, the sample adding part 82 is further provided with a tearing ring 823, and the tearing ring 823 is used for limiting rotation of the sample adding part 82. Tear pull ring 823 is located the outer tube 811 of sample portion 81 between application of sample cap 822, adopts the point-break connection to tear pull ring 823 outwards extends and is equipped with the operation section of being convenient for to tear. The outer circumference of one end of the cap 822 is provided with a plurality of ribs for facilitating the rotary pressurizing operation. The outer circumferences of the sample part 81 and the reaction part 83 are also provided with a plurality of friction ribs for easy operation during rotation.

Before the rapid detection device of the present invention detects, the reaction systems are respectively embedded in the reaction chamber 833, the reaction systems are in a freeze-dried state, the reaction systems are embedded in the reaction chamber through an isolation layer (for example, paraffin) which can be dissolved by heat, and the sample preservation solution is encapsulated in the cavity of the inner tube 812 of the sample portion 81. The sample to be tested is sampled to dip secretion or saliva, the reaction part and the sample part are opened, the sealing piece is removed, the swab section with the sample is arranged in the sample part 81 of the reactor, the reaction part 83 and the sample adding part 82 are screwed down, the tearing pull ring 823 is torn off, the sample adding part 82 is rotated to pressurize the sample liquid, and the sample liquid is forced to flow into the reaction cavity 833 through the micropores 8342. Then the reactor 8 is put into a bearing cavity 11 of the micro-amplification instrument, a cover is covered, then the heating part 2 works to heat the reactor, the heating temperature reaches 60-65 ℃, the heating part 2 stops heating, the centrifugal part 3 works to enable the reactor to rotate and carry out centrifugal motion, and the sample liquid and the reaction system vibrate and are uniformly mixed to react. When the temperature sensor detects that the temperature is lower than 60 ℃, the centrifugal part 3 stops working, the heating part 2 works, and the developed reactor is photographed after the reaction. In the present embodiment, an expensive high-power battery may be used as the rechargeable battery, and when a lower-power battery is used to reduce the cost, a manner of operating the heating part 2 and the centrifugal part 3 at intervals is used to enable the heating part 2 and the centrifugal part 3 to operate normally better. If the battery can supply power to the heating part 2 and the centrifugal part 3 at the same time, centrifugal rotation can be performed while heating.

Example 2

FIG. 4 shows a second embodiment of the nucleic acid amplification reaction vessel of the present invention, which is different from example 1 mainly in the structure of the sample addition part 2, and further, the reaction part 3 and the sample part 1 are formed in an integrated structure.

In this embodiment, the sample adding part 2 comprises a sample adding plug 824 and a sample adding cover 825, the sample adding cover 825 is provided with an inner cavity, the sample adding plug 824 is located in the inner cavity of the sample adding cover 825, and the sample adding plug 824 has resistance unidirectional movement in the inner cavity. The top and bottom of the plug 824 are sealed in the cavity of the lid 825, so that the plug 824 cannot move out of the lid 825. The sample adding plug 824 and the inner cavity of the sample adding cover 825 constitute a piston structure, and a notch communicated with the inner cavity is formed above the sample adding cover 825, so that a tool can be conveniently adopted to apply force to the sample adding plug 824. A vent hole is formed below the sample addition cover, the vent hole is communicated with the sample part 1, and when the sample addition plug 824 moves towards the sample part 1, the sample liquid is pressurized through the vent hole, so that the sample liquid flows towards the reaction cavity 33. In this embodiment, the main body of the sample adding plug 824 is made of a hard transparent material, and sealing rubber rings are arranged at the lower end and the middle part.

In this embodiment, the reaction part 3 and the sample part 1 are formed integrally, and the sample part 1 and the sample addition lid 825 are screwed together.

This example was used in the same manner as example 1.

The above embodiments are merely preferred embodiments of the present invention, which is not intended to limit the present invention in any way. Although the present invention has been described with reference to the preferred embodiments, it is not intended to be limited thereto. Therefore, any simple modification, equivalent change and modification made to the above embodiments according to the technical spirit of the present invention should fall within the protection scope of the technical scheme of the present invention, unless the technical spirit of the present invention departs from the content of the technical scheme of the present invention.

Claims

1. The micro amplification instrument is characterized by comprising a bearing part, a heating part, an installation frame body and a shell, wherein the bearing part and the heating part are arranged in the shell through the installation frame body; the bearing part is provided with a bearing cavity position for placing a sample to be tested; the heating part is arranged around the periphery of the bearing cavity position for electric heating, and the micro-amplification instrument further comprises a centrifugal part which drives the bearing part to rotate and do centrifugal motion.

2. The micro-amplification apparatus of claim 1, wherein the carrier is provided with a carrier cylinder, and the inner cavity of the carrier cylinder forms a carrier cavity.

3. The micro amplification apparatus of claim 2, further comprising a charging power supply device, wherein the centrifugal part is connected to the bearing cylinder via a transmission belt, the charging power supply device is electrically connected to the centrifugal part for supplying power, the centrifugal part drives the bearing cylinder to rotate, and a bearing is disposed between the bearing cylinder and the mounting frame.

4. The micro amplification apparatus of claim 2, wherein the support cylinder is a hollow cylinder or the bottom of the support cylinder is made of a transparent material, and the amplification apparatus further comprises a photographing part, wherein the photographing part is located at the bottom of the support cylinder.

5. A reactor is characterized in that a sample to be detected is placed in the reactor; the reactor comprises a sample adding part, a sample part and a reaction part which are sequentially connected, wherein the sample adding part is movably connected with the sample part, the sample adding part is of a piston structure, a sample preserving fluid is pre-filled in the sample part, and a reaction system is filled in the reaction part; the connecting part of the reaction part and the sample part is provided with micropores, and the aperture of each micropore is not more than the capillary length of the sample liquid or the sample preservation liquid; under the sealed state of the reactor, the sample adding part moves towards the reaction part by external force to realize that the sample liquid passes through the micropores and is pressed into the reaction part under the sealed state.

6. The reactor according to claim 5, wherein at least one reaction chamber is arranged in the reaction part, an independent reaction system is preset in each reaction chamber, the micropores are arranged at the orifice of the reaction chamber, and each reaction chamber corresponds to one micropore.

7. The reactor according to claim 5, wherein the reaction part is provided with a shunt plug made of a flexible material, the shunt plug is positioned at the joint of the reaction part and the sample part, the reaction chamber is independently sealed by the shunt plug, and the micropores are formed in the shunt plug.

8. The reactor of claim 7, wherein the pores have a diameter of 0.3 to 0.6mm.

9. The reactor of claim 5, wherein the sample loading part comprises a sample loading plug and a sample loading cover, the sample loading cover is provided with an inner cavity, the sample loading plug is positioned in the inner cavity of the sample loading cover, the sample loading plug has resistance and moves in a single direction in the inner cavity, the sample loading plug and the inner cavity of the sample loading cover form a piston structure, and the sample solution enters the reaction cavity by the movement of the sample loading plug towards the direction of the sample part in a sealed state of the reactor.

10. A pocket type rapid examination apparatus, characterized in that the apparatus comprises the amplification apparatus according to any one of claims 1 to 4 and the reactor according to any one of claims 5 to 9.