CN215757320U

CN215757320U - Reagent bottle and detection system

Info

Publication number: CN215757320U
Application number: CN202121684992.6U
Authority: CN
Inventors: 朱耀辉; 陈坤
Original assignee: Guangzhou Boda Boju Technology Co ltd
Current assignee: Guangzhou Boda Boju Technology Co ltd
Priority date: 2021-07-23
Filing date: 2021-07-23
Publication date: 2022-02-08
Anticipated expiration: 2031-07-23

Abstract

The utility model discloses a reagent bottle and a detection system, wherein the reagent bottle comprises: the device comprises a pipe body and a pipe cap, wherein a sleeve is arranged in the pipe body, a sample cavity is constructed in the sleeve, a waste liquid cavity is constructed between the sleeve and the pipe body, an extraction cavity is arranged at the first end of the pipe body, the waste liquid cavity and the sample cavity are both communicated with the extraction cavity through a micro-channel, and a puncture structure is arranged in the pipe body; the tube cap covers the second end of the tube body, a closed reagent cavity is arranged in the tube cap, and the tube cap is movably connected with the tube body so as to be suitable for the puncture structure to puncture the reagent cavity and enable the reagent cavity to be communicated with the sleeve for closed tube extraction. According to the reagent bottle provided by the embodiment of the utility model, the tube body is sealed by the tube cap, and the reagent is added by moving the tube cap, so that the extraction and reaction under the closed tube are completed, and the pollution to a sample or the environment is avoided.

Description

Reagent bottle and detection system

Technical Field

The utility model relates to the technical field of medicine, in particular to a reagent bottle and a detection system with the same.

Background

In the medical diagnosis industry, the detection result of the reagent is often needed to diagnose the disease condition, such as new coronary pneumonia. For accuracy and stability considerations, it is often necessary to collect the sample by a swab and subsequently extract the sample with reagents. The existing detection device has larger volume, needs to be carried out in different negative pressure spaces in the detection process, and easily influences the detection result due to the adoption of an open detection mode, and possibly causes the pollution of the surrounding environment.

SUMMERY OF THE UTILITY MODEL

One objective of the present invention is to provide a reagent bottle, in which a tube body is sealed by a tube cap, and reagent is added by moving the tube cap, so as to complete extraction and reaction under the closed tube, and avoid sample contamination or environmental pollution.

Another objective of the present invention is to provide a detection system, which includes the aforementioned reagent bottle.

According to the embodiment of the utility model, the reagent bottle comprises: the device comprises a pipe body and a pipe cap, wherein a sleeve is arranged in the pipe body, a sample cavity is constructed in the sleeve, a waste liquid cavity is constructed between the sleeve and the pipe body, a drawing cavity is arranged at the first end of the pipe body, the waste liquid cavity and the sample cavity are communicated through a micro-channel, the drawing cavity is provided with a puncture structure in the pipe body, the pipe cap covers the second end of the pipe body, a closed reagent cavity is arranged in the pipe cap, and the pipe cap is movably connected with the pipe body so as to be suitable for the puncture structure to puncture the reagent cavity and enable the reagent cavity to be communicated with the sleeve for closed-tube drawing.

According to the reagent bottle provided by the embodiment of the utility model, the tube body is sealed by the tube cap, and the reagent is added by moving the tube cap, so that the extraction and reaction under the closed tube are completed, and the pollution to a sample or the environment is avoided.

In addition, the reagent bottle according to the above embodiment of the present invention may further have the following additional technical features:

optionally, a plurality of reagent chambers are arranged at intervals in the tube cap, and the plurality of reagent chambers are suitable for being punctured by the puncturing structure in sequence and are communicated with the sleeve in sequence.

Optionally, the tube cap is movably connected to the tube body along an axial direction of the tube body, and the plurality of reagent chambers are arranged at intervals along an axial direction of the tube cap.

Optionally, be equipped with the inner tube in the pipe cap, the inner tube is followed the axis of body extends, the inner tube is kept away from sheathed tube one end is sealed and is opened towards sheathed tube one end, be equipped with a plurality of diaphragms in the inner tube, it is a plurality of the diaphragm is followed the axis direction interval of body sets up, every adjacent two between the diaphragm and the diaphragm with all construct between the blind end of inner tube the reagent chamber.

Optionally, the diaphragm includes a hard film layer and a flexible film layer, the hard film layer is connected to the periphery of the flexible film layer, the hard film layer and the flexible film layer are connected to form a flat plate, the flexible film layer is opposite to the sleeve in the axial direction of the pipe body, and the thickness of the flexible film layer is smaller than that of the hard film layer.

Optionally, the flexible film layer and the hard film layer are configured into a concave structure with an opening facing the sleeve.

Optionally, a plurality of strip-shaped grooves are formed in the surface of the flexible film layer, and the strip-shaped grooves are connected to one position and are configured into a divergent shape.

Optionally, the inner tube is configured as a reducing tube with a gradually decreasing radial dimension in a direction away from the sleeve, and a plurality of steps are configured on an inner circumferential surface of the inner tube, and the diaphragm is disposed in the sleeve and supported on the steps on the inner circumferential surface of the inner tube.

Optionally, an annular clamping groove is formed in the inner end face of the pipe cap, an annular clamping hook is arranged at the end of the inner pipe, and the annular clamping hook is clamped with the annular clamping groove.

Optionally, the tube cap is rotatably connected to the tube body, and the plurality of reagent chambers are arranged at intervals along the circumference of the tube cap.

Optionally, a sample preservation solution, a lysis solution, a first cleaning solution, a second cleaning solution and an air column are sequentially arranged in the pipe cap along the puncture sequence of the puncture structure.

Optionally, the end portion of the sleeve is configured with the puncture structure, and the end portion of the sleeve is provided with a plurality of notch grooves arranged at intervals along the circumferential direction of the sleeve.

Optionally, the cap is threadedly engaged with the body.

Optionally, the pipe cap is sleeved outside the pipe body, first coarse threads are arranged on the inner circumferential surface of the pipe cap, second coarse threads are arranged on the outer circumferential surface of the pipe body, and the first coarse threads are matched with the second coarse threads.

Optionally, a fine thread is further disposed on the outer circumferential surface of the tube body, the fine thread is matched with the second coarse thread, and the first coarse thread is in contact with the fine thread when the sleeve pierces the reagent chamber.

Optionally, a sealing rib is arranged on an inner end face of the pipe cap, and the sealing rib is suitable for abutting against an inner peripheral edge of the second end of the pipe body, so that the pipe cap is in sealing fit with the pipe body.

Optionally, an anti-drop groove is formed in the outer peripheral surface of the first end of the pipe body, and the anti-drop groove is formed along the circumferential direction of the pipe body.

Optionally, a plurality of tooth grooves are formed in the outer peripheral surface of the pipe cap, the tooth grooves are parallel to the axis of the pipe body, and the tooth grooves are arranged at intervals along the circumferential direction of the pipe cap.

Optionally, the outer circumferential surface of the first end of the pipe body is provided with anti-slip ribs, the anti-slip ribs extend in a direction parallel to the axis of the pipe body, and a plurality of anti-slip ribs are arranged at intervals along the circumferential direction of the pipe body.

Optionally, an extraction membrane is arranged in the extraction cavity, the waste liquid cavity is communicated with the inner side surface of the extraction membrane, and the sample cavity is communicated with the outer side surface of the extraction membrane.

According to the detection system of the embodiment of the utility model, the detection system comprises: the reagent bottle comprises a positioning module, a heating module, a shooting module and a reagent bottle, wherein the reagent bottle is the reagent bottle according to the above description, the positioning module is provided with an insertion opening, the first end of the reagent bottle is suitable for being inserted into the insertion opening and is positioned by the positioning module, the heating module is used for heating the extraction cavity, and the shooting module is opposite to the end face of the first end of the reagent bottle so as to be suitable for obtaining a fluorescence quantitative image.

Drawings

FIG. 1 is a schematic diagram of a detection apparatus of one embodiment of the present invention.

FIG. 2 is a schematic view of a detection apparatus according to an embodiment of the present invention with the housing removed.

FIG. 3 is a partial schematic view of a detection apparatus of one embodiment of the present invention.

FIG. 4 is a schematic view of a portion of a detection apparatus of one embodiment of the present invention in another orientation.

FIG. 5 is a schematic view of a mounting seat and a positioning assembly of the inspection apparatus according to an embodiment of the present invention.

Fig. 6 is a schematic view of a mounting seat, a heating element and a hot air module of the detection device according to an embodiment of the utility model.

FIG. 7 is a schematic view of a mounting block of a detection apparatus of one embodiment of the present invention.

FIG. 8 is a partial schematic view of a detection apparatus of one embodiment of the present invention.

Fig. 9 is a schematic view of the structure of fig. 8 with the mounting housing removed.

FIG. 10 is a schematic diagram of the combination of the turntable, probe and filter assembly in the inspection apparatus according to one embodiment of the present invention.

FIG. 11 is a schematic diagram of the combination of the turntable, probe, filter assembly and sensing element in the inspection apparatus of one embodiment of the present invention.

FIG. 12 is a schematic view of the inner side of the power connection structure of the detection apparatus according to one embodiment of the present invention.

FIG. 13 is a schematic view of a reagent bottle according to one embodiment of the present invention.

FIG. 14 is a cross-sectional view of a reagent bottle according to one embodiment of the present invention.

Fig. 15 is a partially enlarged schematic view of the area circled a in fig. 14.

FIG. 16 is a cross-sectional view of a reagent bottle according to one embodiment of the present invention.

FIG. 17 is a schematic view of a septum of a reagent bottle according to one embodiment of the present invention.

FIG. 18 is a schematic view of a septum of a reagent bottle according to an embodiment of the present invention.

Fig. 19 is a schematic view of a tube of a reagent bottle according to an embodiment of the present invention.

Fig. 20 is a schematic view of a tube of a reagent bottle according to an embodiment of the present invention.

Fig. 21 is a schematic view of a tube of a reagent bottle according to an embodiment of the present invention.

FIG. 22 is a schematic view of the inside face of the inner end plate of a reagent bottle according to one embodiment of the present invention.

FIG. 23 is a schematic view of the outside face of the inner end plate of a reagent bottle according to one embodiment of the present invention.

FIG. 24 is a schematic view of the inside face of the outer end plate of a reagent bottle according to one embodiment of the present invention.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the utility model and are not to be construed as limiting the utility model.

The detecting device 200 according to the embodiment of the present invention includes a positioning module, the positioning module is provided with an insertion opening 201, the insertion opening 201 is used for inserting the reagent bottle 100, after the reagent bottle 100 is inserted, the operations such as locking, heating, heat sealing, quantitative fluorescence detection and the like can be performed on the reagent bottle 100, wherein after the reagent bottle is inserted into the insertion opening, the reagent bottle can be locked through the positioning module, and different operation devices of the reagent bottle 100 are respectively described below with reference to the attached drawings.

With reference to fig. 1 to 7, a device for heating a reagent bottle 100 according to an embodiment of the present invention is described, which includes a plurality of heating members 22 and a driving device 23, wherein the plurality of heating members 22 are respectively movable to selectively heat an extraction film in the reagent bottle 100, that is, each of the plurality of heating members 22 can heat the extraction film in the reagent bottle 100, and in general, one or more of the plurality of heating members 22 can be used to heat the reagent bottle 100 in order to facilitate control of a heating temperature, so as to avoid damage to a reaction reagent, avoid fatigue, and improve a service life of the detection apparatus 200. A drive 23 is connected to the heating member 22 to drive the heating member 22 in movement.

According to the detection device 200 of the embodiment of the utility model, the plurality of heating members 22 are arranged to selectively heat the extraction film in the reagent bottle 100, so that different detection temperatures can be met, the application range of the detection device 200 is improved, and meanwhile, the detection under various temperature conditions in the same detection target can be met.

The heating member 22 in the present invention can be moved in a plurality of directions to approach and separate from the reagent bottle 100, that is, to switch between heating the reagent bottle 100 and not heating the reagent bottle 100, for example, the heating member 22 can be rotated to approach and separate from the reagent bottle 100; it is also possible to provide for movement in a radial direction of the insertion hole to achieve access to and removal from the reagent bottle 100, and in addition, some embodiments are provided to achieve heating of the reagent bottle 100.

In some embodiments of the present invention, the heating member 22 is movably provided on the positioning module in a direction parallel to the axis of the insertion port 201. That is, the heating member 22 moves in a direction parallel to the axis of the insertion port 201 to be close to and away from the reagent bottle 100, thereby realizing heating and non-heating of the reagent bottle 100, preventing the heating member 22 from interfering with other devices (for example, a device for rotating a cap, a device for locking a tube, etc., described below), improving the stability of movement of the heating member 22, simplifying the structure of the detecting apparatus 200, and facilitating design, production, and maintenance of the detecting apparatus 200.

As shown in fig. 5, the heating member 22 has a heating end for heating the reagent bottles 100, the heating end being adapted to be close to or abut against the reagent bottles 100 for heating the reagent bottles 100. The heating ends are opposed to the insertion port 201 along the axis of the insertion port 201, and the projections of the heating ends of the plurality of heating members 22 in the direction of the axis of the insertion port 201 are configured in a ring shape having an observation port. Therefore, the reagent bottle 100 can be conveniently heated, meanwhile, the observation port is arranged, the interference of a detection device (such as a probe 272 described below) to the examination of the reagent bottle 100 can be avoided, and under the condition of convenient heating, the observation can be conveniently carried out, so that the detection efficiency is improved.

As shown in fig. 6, the driving device 23 includes a plurality of heating members 22 corresponding to the plurality of heating members 22 one by one, and the driving device 23 includes a heating motor and a heating cam, the heating cam is rotatably embedded in the corresponding heating member 22, and the heating motor is connected to the heating cam for driving the corresponding heating member 22 to move. Specifically, the heating member 22 may be provided with an insertion hole, the size of the insertion hole may be larger than that of the heating cam, and the heating cam is in a non-revolving body shape with respect to the motor shaft of the heating motor, so that the outer peripheral surface of the heating cam has a position with a different distance from the motor shaft of the heating motor, thereby driving the heating member 22. Of course, other arrangements known in the art may be used to drive the heating member 22 in the present invention.

As shown in fig. 6 and 7, the positioning module includes a mounting seat 26, the heating element 22 and the driving device 23 are both disposed on the mounting seat 26 and away from the insertion opening 201, a fitting hole 203 is disposed on the mounting seat 26, and the heating element 22 is adapted to extend into the fitting hole 203 to heat the reagent bottle 100. By providing the mounting base 26, the integration of the heating device can be facilitated, so that the assembly and maintenance of the detection apparatus 200 can be facilitated, and the stability of the movement of the heating member 22 can be ensured.

As shown in fig. 5, the mounting seat 26 is provided with a positioning member opposite to the insertion port 201 and adapted to receive and position the end of the reagent bottle 100. In the use, the reagent bottle inserts from inserted hole 201, and the terminal locating component that inserts of reagent bottle fixes a position the reagent bottle through locating component to the body that realizes the reagent bottle is fixed, thereby conveniently rotates the pipe cap.

Wherein, as shown in fig. 5, the positioning assembly may comprise a positioning ring 211, the positioning ring 211 being adapted to receive the end of the reagent bottle 100. During use, the end of the reagent bottle is inserted into the positioning ring 211.

Alternatively, as shown in fig. 5, the positioning ring 211 is provided on its inner peripheral surface with a positioning rib 212, the positioning rib 212 extending in a direction parallel to the axis of the insertion port 201, the positioning rib 212 being for positioning the reagent bottle 100 in the circumferential direction of the insertion port 201. Correspondingly, be equipped with the constant head tank on the bottle of reagent bottle 100, the constant head tank can be for the structure with location muscle 212 adaptation, in the assembling process, can insert reagent bottle 100 into inserted hole 201, and location muscle 212 inserts the constant head tank simultaneously to realize the fixed of the bottle of reagent bottle 100. Wherein the positioning rib 212 may be disposed in an asymmetric manner with respect to the axis of the insertion opening 201 such that the reagent bottle 100 will be inserted into the insertion opening 201 in a specific orientation, for example, when performing a heat sealing process, the insertion of the heat sealing needle into the reagent bottle 100 may be facilitated by the orientation restriction of the reagent bottle 100 to complete the heat sealing of the corresponding opening in the reagent bottle 100.

Optionally, as shown in fig. 5, the positioning assembly further comprises a latch 213, the latch 213 is movably connected to the mounting seat 26 along a radial direction of the positioning ring 211, and the latch 213 is used for positioning the reagent bottle 100 along an axial direction of the insertion port 201. Correspondingly, the end of the reagent bottle 100 may be provided with a slip-off preventing slot, and the latch 213 may be inserted into the slip-off preventing slot during use, so as to position the reagent bottle 100 along the axial direction of the insertion port 201 and prevent the reagent bottle 100 from slipping off from the insertion port 201.

In the present invention, the reagent may be dispensed, or the reagent and the sample may be mixed, reacted, or the like, when the reagent bottle 100 is inserted into the testing device 200, or the reagent bottle 100 may be inserted into the testing device 200, and the actual dispensing may be realized by a corresponding structure on the testing device 200.

Referring to fig. 3 and 4, in some embodiments of the present invention, the positioning module further includes a rotary cylinder 214 and a driving structure 215, the rotary cylinder 214 has an insertion opening 201 formed therein, the rotary cylinder 214 is rotatable around an axis of the insertion opening 201, the driving structure 215 is connected to the rotary cylinder 214 to drive the rotary cylinder 214 to rotate, and the rotary cylinder 214 is adapted to lock the cap of the reagent bottle 100 to drive the cap to rotate. In the using process, the reagent bottle 100 is inserted into the positioning module, wherein the reagent bottle 100 comprises a tube body and a tube cap, and the reagent is put in by rotating the tube cap relative to the tube body. Through preceding locating component, can realize the fixed of reagent bottle 100's body, and pin the pipe cap and rotate through rotatory bucket, can be in order to realize the rotation of pipe cap for the body to accomplish the input of reagent. Therefore, by providing the rotary cylinder 214 and the driving structure 215, automatic dispensing of the reagent in the reagent bottle 100 can be achieved.

Alternatively, the heating element 22 of the present invention is a thermostatic module. Can realize extracting the heating of membrane in reagent bottle 100 through the constant temperature module to in will extracting the membrane heating to predetermined temperature in order to accomplish the detection, improve the detection effect, avoid simultaneously because the fatigue that the rate of heating leads to at the excessive speed, improve structural strength and life.

In addition, the present invention provides some technical solutions to realize the heat sealing of the opening in the reagent bottle 100, in some embodiments of the present invention, the detecting device 200 further includes: the heat-sealing device 24 and the heat-sealing drive 25 are arranged, the heat-sealing device 24 and the insertion port 201 are opposite along the axial direction of the insertion port 201, the heat-sealing device 24 is movably arranged in the positioning module along the axial direction of the insertion port 201, and the heat-sealing device 24 comprises a heat-sealing needle which is suitable for being inserted into the reagent bottle 100 for heat sealing. The heat-seal drive 25 is arranged on the positioning module, and the heat-seal drive 25 is connected with the heat-seal device 24 to drive the heat-seal device 24 to move. After the reagent bottle 100 is inserted into the testing apparatus 200, the heat-sealing needle can be inserted into the reagent bottle 100 by moving the heat-sealing device 24, so that the heat sealing of the corresponding opening in the reagent bottle 100 is completed, thereby improving the accuracy of the testing result.

Alternatively, as shown in fig. 6, the heat-seal driving unit 25 includes a heat-seal motor and a heat-seal cam, the heat-seal cam is rotatably embedded in the heat-seal device 24, and the heat-seal motor is connected to the heat-seal cam for moving the heat-seal device 24. The heat-seal drive 25 is driven in substantially the same manner as the heating drive.

Optionally, in combination with the foregoing description, the positioning module includes a mounting seat 26, the heat sealing device 24 is disposed on the mounting seat 26 and faces away from the insertion opening 201, a fitting hole 203 is formed on the mounting seat 26, and the heat sealing device 24 is adapted to extend into the insertion opening 201 through the fitting hole 203. So that the integration of each component in the detection device 200 is facilitated, and the assembly and maintenance are facilitated. In connection with the previous embodiment, heat sealing device 24 and heating element 22 are both disposed on mounting base 26.

Referring to fig. 5, in some embodiments of the present invention, a limiting plate 261 is disposed in the fitting hole 203, the limiting plate 261 has a hollow ring shape, and the limiting plate 261 is provided with a guide hole 262 corresponding to the heat-sealing pin. In order to realize the direction to the heat-seal needle, improve the stability of heat-seal effect, wherein, can also set up the guide cylinder on the limiting plate 261, the guide cylinder is hollow tubular structure, and the heat-seal cover can pass in following the guide cylinder to lead to the heat-seal needle through the guide cylinder. And the guide cylinder is embedded in the heating element 22, thereby realizing the guide of the heating element 22 through the guide cylinder, simultaneously realizing the guide of the heating cylinder and the heat sealing needle through the guide cylinder, and simplifying and optimizing the structure.

In addition, the testing apparatus 200 of the present invention further comprises a device for observing the reagent bottle 100, and finally gives a reasonable and accurate conclusion by observing the reagent bottle 100 under different temperature and other conditions.

Referring to fig. 8 to 12, in some embodiments of the present invention, the photographing module includes a turntable 271, a probe 272, a plurality of filter members 273, and a sensing element 274, the turntable 271 is rotatably connected to the positioning module, the probe 272 and the positioning module are relatively fixed and opposite to the end of the insertion port 201, the plurality of filter members 273 are arranged at intervals around a rotation axis of the turntable 271, the plurality of filter members 273 are driven by the turntable 271 to be alternatively opposite to the probe 272, and the sensing element 274 is configured to cooperate with the probe 272 to capture light passing through the filter members 273. In the using process, the reagent bottle 100 can be observed by using different filter assemblies 273 through the rotation of the turntable 271, so as to meet the detection requirement and improve the precision of the detection result.

Wherein, a sensing element 274 can be correspondingly disposed on each filter assembly 273 to improve the detection efficiency, and a sensing element 274 can be disposed to observe the form effect under different filter assemblies 273. Alternatively, in conjunction with fig. 10 and 11, the sensing element 274 is fixed relative to the positioning module and is opposite the probe 272, and the plurality of filter assemblies 273 are carried by the turntable 271 to alternatively be opposite the probe 272 and the sensing element 274. Through fixing the sensing element 274 and the positioning module, the wiring of the sensing element 274 can be simplified, the phenomenon that a wiring harness is wound in the rotating process of the turntable 271 is avoided, the stability of signal transmission is effectively improved, meanwhile, the number of the sensing elements 274 can be reduced, and the cost is reduced.

With reference to fig. 11 and 12, in some embodiments of the present invention, a plurality of power connection structures insulated from each other are disposed on the turntable 271, and the power connection structures include: the positioning module comprises an insulating column 281, a power connection ring 282 and a conductive member 283, wherein the insulating column 281 is fixed relative to the positioning module, the power connection ring 282 is rotatably sleeved on the insulating column 281, the conductive member 283 is arranged on the insulating column 281 and is slidably abutted against the power connection ring 282, and the conductive member 283 is electrically connected with the power connection ring 282; wherein the filter assembly 273 is electrically connected to the power ring 282. The conductive member 283 can be connected with a power supply through a wire harness, and the conductive member 283 and a corresponding structure on the positioning module are electrically connected and positioned due to the fixed connection of the insulating column 281 and the positioning module, so that the problem of wire winding cannot occur. And connect electrical ring 282 can with carousel 271 fixed connection, owing to adopted the form that electrically conductive 283 butt connects electrical ring 282, both are at the in-process of relative movement, also can realize stable electricity and connect to can realize electrically conductive 283's stability, and then realize that filter assembly 273 connects the power.

In addition, an insulating ring 284 may be disposed between the conductive member 283 and the turntable 271 to electrically isolate the conductive member 283 from the turntable 271, thereby improving the safety of the electrical connection structure.

The insulating column 281 is a hollow structure, and a through hole is formed in the circumferential surface of the insulating column 281, the conductive piece 283 is a ball plunger which is arranged in the insulating column 281 and extends out of the through hole, and a ball of the ball plunger abuts against the conductive piece 283. Thereby improving the stability of the conductive structure.

Alternatively, as shown in FIG. 11, the turntable 271 includes a hub 2711 and a plurality of filter receptacles 2712, the plurality of filter receptacles 2712 being connected to the hub 2711 and being disposed about the hub 2711, the plurality of filter assemblies 273 being mated with the plurality of filter receptacles 2712 in a one-to-one correspondence.

In addition, the relative both ends of wheel hub 2711 are equipped with the mounting groove, and install in the mounting groove and connect electric structure.

Referring to FIG. 8, in some embodiments of the present invention, the detecting apparatus further comprises a supporting base 208 and a mounting housing 209, wherein the mounting housing 209 covers the supporting base 208, wherein the sensing element 274 is disposed on the supporting base 208, the probe 272 is disposed on the mounting housing 209, and the turntable 271 and the plurality of filter assemblies 273 are disposed in the mounting housing 209. Therefore, stable installation of each element can be realized, and the structure and the stability are optimized.

In addition, two insulating posts 281 of the electrical connection structure may be connected to the supporting base 208 and the mounting case 209, respectively. Thereby achieving a stable connection of the insulating columns 281 with the positioning module.

Alternatively, a circular cavity may be provided in the mounting housing 209, with the turntable 271 being rotatably disposed within the circular cavity. The turntable 271 can be positioned by the circular cavity, guiding the stable rotation of the turntable 271.

Optionally, a rotating shaft is disposed on the supporting seat 208, the rotating plate 271 includes a rotating plate seat rotatably connected to the rotating shaft and a plurality of mounting slots disposed around the rotating shaft for mounting the filter assembly 273, and a rotating plate cover covering the mounting slots. By providing the turntable base and the turntable cover, the filter assembly 273 can be prevented from being damaged by other components, the influence of the surrounding environment on the observation result can be effectively eliminated, and the precision of the observation result can be improved.

Optionally, the turntable cover comprises a bottom plate and a plurality of partition plates, the bottom plate covers the turntable base, and holes corresponding to the plurality of filter assemblies 273 one by one are formed in the bottom plate; the baffle links to each other and extends towards the carousel seat with the bottom plate, and a plurality of baffles set up along the circumference interval of bottom plate, all is equipped with the baffle between every two adjacent filter assembly 273. Through the bottom plate, can realize the encapsulation of a plurality of filter element 273, and through setting up the baffle, can conveniently separate a plurality of filter element 273, reduce simultaneously impurity such as dust and enter into filter element 273 in, improve the precision of testing result.

Referring to fig. 11, in some embodiments of the present invention, the turntable 271 further includes an outer ring gear 2713, the outer ring gear 2713 surrounds and is fixedly connected to the turntable base, the supporting base or the mounting housing is provided with a turntable motor, the turntable is driven by the turntable motor, and the turntable motor is in transmission connection with the outer ring gear 2713. Therefore, the rotating disc 271 can be conveniently driven to rotate, and the stability of the rotating process of the rotating disc 271 is improved.

Referring to fig. 1 to 12, in combination with the previous embodiment, the positioning module further includes a mounting seat 26, a heating device for heating the reagent bottle 100 is disposed on the mounting seat 26, the mounting seat 26 is disposed between the turntable 271 and the insertion port 201, a fitting hole 203 is disposed on the mounting seat 26, and the insertion port 201, the fitting hole 203, and the probe 272 are opposite to each other along an axis of the insertion port 201. Heating device can realize the heating to drawing the membrane to obtain good testing result, set up mount pad 26 simultaneously between carousel 271 and inserted hole 201, can simplify heating device and viewing device's structure, and the mating holes 203 that sets up on mount pad 26 can make things convenient for light to see through in order conveniently to observe and improve detection efficiency.

The mounting seat 26 and the supporting seat 208 can be assembled together after being assembled respectively, so that the assembly process is simplified.

Referring to fig. 1, in some embodiments of the present invention, the positioning module includes a housing 21, an insertion opening 201 is exposed from an outer surface of the housing 21, and the housing 21 is provided with a heat dissipation opening communicating with an inner space of the housing 21. Through setting up the thermovent, can conveniently dispel the heat to the components and parts in the orientation module, optimize the operational environment of each components and parts in check out test set 200.

Wherein, orientation module, heating module and shooting module all locate in the casing.

Optionally, the detection apparatus 200 further comprises a display screen 29, and a display surface of the display screen 29 is configured as a part of an appearance surface of the positioning module.

In addition, the detection device further comprises a signal processing module, which is arranged on the positioning module and is in signal transmission with the sensing element 274; the display screen 29 is in signal transmission with the signal processing module. By integrating the display screen 29 into the housing 21, miniaturization and portability of the detection apparatus 200 can be facilitated.

As can be seen from the foregoing description, the detection apparatus 200 of the present invention can be used for PCR detection, and can improve the detection efficiency of PCR and the portability of the detection apparatus 200, wherein the detection process can be divided into the following steps:

mixing of a sample and a reagent is completed in the reagent bottle 100, that is, the reagent is added into a cavity where the sample is located and is extracted and collected through an extraction membrane, the process can be completed before the reagent bottle 100 is inserted into the detection device 200, the reagent bottle 100 can also be inserted into the detection device 200, and the detection device 200 automatically completes the process of reagent feeding, wherein the process of reagent addition and extraction of the extraction membrane is described in detail below;

② after inserting the reagent bottle 100 into the insertion port 201, locking and positioning of the reagent bottle 100 is realized by the structure of the latch 213, the positioning rib 212, etc. so that the reagent bottle 100 can be inserted into the detecting apparatus 200 in a predetermined trajectory and direction.

Inserting the reagent bottle 100 and putting in the completion back of reagent, can carrying out the heat-seal to reagent bottle 100 to seal the die cavity in the reagent bottle 100, go on the actual storage reaction of multicomponent in the die cavity integratively, cut off die cavity and sample chamber and waste liquid chamber.

Fourthly, the extraction film in the reagent bottle 100 may be heated by the heating member 22, and since the extraction film and the outer end plate are both thin, the whole extraction film may be heated by heating one position of the extraction film, wherein three heating members 22 (one, two, or four or more heating members 22 may be provided) may be provided to heat the reagent bottle 100, wherein the heating members 22 may be constant temperature modules, and the heating members 22 may be lifted along the axial direction of the insertion port 201 to heat and not heat the reagent bottle 100, wherein the plurality of heating members 22 are arranged around the insertion port 201 at even intervals, for example, the sago root heating members 22 are arranged at intervals of 120 °, and the plurality of heating members 22 may be programmed to heat the extraction film in the reagent bottle 100, respectively.

After the heating is completed or during the heating process, the reagent bottle 100 can be observed through the probe 272, the filter assembly 273 and the sensing element 274, wherein the filter assembly 273 can be switched by rotating the turntable 271, the filter assembly 273 can be switched by continuous unidirectional rotation, the wavelength switching time is shortened, and power supply can be realized through a slip ring type power supply structure. Among them, five fluorescence filter assemblies 273 of different colors may be provided to improve detection accuracy.

Through the steps, the detection of the reagent can be realized, and the detection efficiency is improved, wherein the utility model miniaturizes the microscopic light path, has a high-sensitivity color camera, and can shoot the fluorescence image of each fluorescence channel by adopting the colorless fluorescence filter assembly 273.

In conjunction with the foregoing testing apparatus 200, the present invention also provides a reagent bottle 100, and the reagent bottle 100 can be applied to the foregoing testing apparatus 200.

As shown in FIG. 13, the present invention also provides a reagent bottle 100, and the reagent bottle 100 can be applied to the aforementioned detection apparatus, and can also be produced, sold and used separately. Including body 11 and pipe cap 12, be equipped with in the body 11 and draw chamber, sample chamber and waste liquid chamber all draw the chamber through the microchannel intercommunication. Wherein, draw the chamber and be used for the reaction and draw, the sample chamber is used for puting in the sample, and the waste liquid chamber is used for collecting the waste liquid. The cap 12 is adapted to close the body 11. Wherein at least one of the tube cap and the tube body is provided with a closed reagent cavity therein, the tube cap is configured to be movable when the tube body is covered so as to be suitable for puncturing the reagent cavity, and the reagent cavity is configured to be suitable for flowing a reagent into the sample cavity and/or the extraction cavity after puncturing. Therefore, in the reagent chamber of the present invention, after the tube cap seals the tube body, the reagent can still be put in by moving the tube cap, so that the closed tube reagent putting, the closed tube extraction and the closed tube reaction are realized.

In addition, with reference to the foregoing embodiment, after the reagent bottle 100 is inserted into the insertion opening, the extraction cavity of the reagent bottle 100 may be opposite to the heating module and the photographing module, so as to heat, photograph, etc. the extraction film of the reagent bottle 100, thereby realizing effective quantitative fluorescence detection, and the process of fluorescence quantitative detection can be completed by one device, thereby simplifying detection.

In some embodiments of the present invention, a sleeve 111 is disposed in the tube 11, an extraction cavity is disposed at a first end (referring to a lower end of the tube 11 in the drawings) of the tube 11, a sample cavity 1102 is formed in the sleeve 111, a waste liquid cavity is formed between the sleeve 111 and the tube 11, and the waste liquid cavity and the sample cavity are both communicated with the extraction cavity via a micro flow channel. The cap 12 covers a second end of the tubular body 11 (refer to an upper end of the tubular body 11 in the drawing). The sample cavity 1102 may be filled with a sample, for example, a collected sample swab a may be placed in the sample cavity 1102, wherein the sample swab a, such as a cotton swab, may be broken in order to reduce the size of the reagent bottle 100, and an effective portion may be placed in the sample cavity 1102. The cap 12 covers the tube 11 and is movable along the tube 11 so that the dispensing of the reagent can be achieved by movement of the cap 12 along the tube 11. Various embodiments are provided to accomplish the administration of the agent, for example, by placing the agent in the cap 12 and piercing it through the cannula 111; also can place reagent in body 11, realize the input of reagent through the removal of pipe cap 12, at the input in-process of reagent, pipe cap 12 seals body 11, can avoid the production of pollutants such as aerosol, avoids the pollution of sample, reagent etc. in the reagent bottle, also can avoid the pollution to external environment. The present invention will be described in detail.

According to the utility model, a closed reagent adding mode is adopted, so that the pollution in the bottle and the pollution to the external environment can be prevented, and the influence of the external environment on the extraction and reaction processes of the reagent bottle is reduced. So that moderate or room temperature preservation and operation can be achieved.

Referring to fig. 14, in some embodiments of the present invention, a closed reagent chamber 1201 is disposed in the tube cap 12, the tube cap 12 is movably connected to the tube body 11, and the tube body further has a puncturing structure, so that the puncturing structure punctures the reagent chamber 1201 during the movement of the tube cap 12 relative to the tube body 11 and enables the reagent chamber 1201 to communicate with the cannula, so that when the tube cap 12 closes the tube body 11, the operations of reagent feeding, sample extraction, and the like can still be achieved, and the tube closure extraction can be achieved. That is, during the movement of the cap 12, the piercing structure in the tube body also moves relative to the cap 12 and pierces the reagent chamber 1201 in the cap 12, and when the reagent chamber 1201 in the cap 12 is pierced, the reagent chamber 1201 can be communicated with the sample chamber 1102, so that the reagent in the reagent chamber 1201 is put into the sample chamber 1102. Wherein the reagent in the reagent cavity 1201 can be a liquid reagent, so as to facilitate the dispensing of the reagent.

There are various ways to construct the reagent chamber 1201 in the cap 12 according to the present invention, for example, a gas bag is provided in the cap 12, a plurality of partition plates are provided in the cap 12 to construct a plurality of reagent chambers 1201 at intervals, and it is needless to say that in order to facilitate the puncture and communication of the reagent chamber 1201 by the cannula 111, an inner tube 121 may be provided in the cap 12 to construct the reagent chamber 1201, and the present invention will be described in detail below.

In some embodiments of the present invention, only one reagent chamber may be disposed in the cap, or a plurality of reagent chambers may be disposed at intervals in the cap, and the plurality of reagent chambers are adapted to be punctured by the puncturing structure in sequence and to sequentially communicate with the cannula. When a reagent cavity is arranged in the pipe cap, the reagent can be quickly put in after the reagent cavity is punctured, and when a plurality of reagent cavities are arranged in the pipe cap, the reagent can be actually put in the reagent cavities respectively and successively.

Wherein, a plurality of reagent chambeies in the tube cap can adopt the mode of multiple difference to set up to the structure of puncturing punctures conveniently.

Optionally, the tube cap is movably connected with the tube body along the axial direction of the tube body, and the plurality of reagent chambers are arranged at intervals along the axial direction of the tube cap. At this moment, move along the axis direction of body through the pipe cap, can realize pricking a plurality of reagent chambeies in proper order to communicate a plurality of reagent chambeies in proper order the sample chamber, and realize the input of reagent.

As shown in fig. 14 to 16, an inner tube 121 is disposed in the cap 12, the inner tube 121 extends along the axis of the tube 11, a plurality of reagent chambers 1201 are disposed at intervals along the axis of the tube 11 in the inner tube 121, and the inner tube 121 is adapted to be inserted into the tube 11 from the second end of the tube 11 so as to be adapted to be pierced by the cannula 111 in sequence and communicate with the plurality of reagent chambers 1201. Through set up a plurality of reagent chambeies 1201 in inner tube 121, can make things convenient for the input of reagent in the reagent chamber 1201, and a plurality of reagent chambeies 1201 set up along the axis interval of body 11, consequently, at the in-process that removes pipe cap 12, sleeve pipe 111 will puncture a plurality of reagent chambeies 1201 in proper order to realize putting in respectively of reagent chamber 1201, satisfy different detection requirements.

Optionally, with reference to fig. 14 to 16, an end of the inner tube 121 away from the sleeve 111 is closed, a plurality of diaphragms 122 are disposed in the inner tube 121, the plurality of diaphragms 122 are spaced along the axial direction of the tube 11, and a reagent chamber 1201 is formed between each two adjacent diaphragms 122 and between the diaphragm 122 and the closed end of the inner tube 121. By arranging the membrane 122, the puncture of the cannula 111 can be facilitated, the puncture of the cannula 111 and the connection of the reagent chamber 1201 are further facilitated, and the reagent feeding efficiency is improved.

Further, as shown in fig. 17 and 18, the diaphragm 122 includes a hard film layer 122a and a flexible film layer 122b, the hard film layer 122a is connected to the periphery of the flexible film layer 122b, the hard film layer 122a and the flexible film layer 122b are connected to form a flat plate, the flexible film layer 122b is opposite to the sleeve 111 in the axial direction of the pipe body 11, and the thickness of the flexible film layer 122b is lower than that of the hard film layer 122 a. The flexible film 122b with smaller thickness is opposite to the sleeve 111 during the use process, therefore, the sleeve 111 will easily puncture the flexible film 122b during the process of moving the cap 12, further improving the dispensing efficiency of the reagent.

Optionally, the flexible film layer 122b and the hard film layer 122a are configured as a concave structure opening toward the sleeve 111. During use, the end of the sleeve 111 will penetrate into the recessed structure, and guiding of the sleeve 111 can be achieved through the recessed structure, so that the sleeve 111 can rapidly and stably puncture the flexible film layer 122b, and can also play a certain sealing role, so that the reagent in the reagent chamber 1201 can be stably released into the sample chamber 1102.

Optionally, with reference to fig. 17 and 18, a plurality of grooves 122c are formed on the surface of the flexible film layer 122b, and the plurality of grooves 122c are connected together and configured to be divergent. Through setting up bar groove 122c for flexible rete 122b is punctureed more easily, improves the efficiency that reagent was put in. As can be seen from fig. 17 and 18, at least one side surface of the flexible film layer 122b is provided with a strip-shaped groove 122c, and in addition, the outer side surface of the outermost membrane 122 on the inner tube is provided with a plurality of annular ribs, and the plurality of annular ribs may extend in a plurality of concentric circles centering on the sleeve.

Alternatively, referring to fig. 14 to 16, the inner tube 121 is formed in a tapered tubular shape having a radial dimension gradually decreasing in a direction away from the sleeve 111, a plurality of steps are formed on an inner circumferential surface of the inner tube 121, and the diaphragm 122 is disposed in the sleeve 111 and supported on the steps on the inner circumferential surface of the inner tube 121. In other words, the inner tube 121 is configured to include a plurality of tube segments, the plurality of tube segments are sequentially connected along the axial direction of the casing 111, and the radial dimension of the tube segment far from the casing 111 is smaller than that of the tube segment near the casing 111, so that a step facing the casing 111 is configured between two adjacent tube segments, and the diaphragm 122 can be supported on the step, and during the process of puncturing the diaphragm 122 by the casing 111, the step can provide support for the diaphragm 122, so that the casing 111 can stably puncture the diaphragm 122, thereby improving the stability of the puncturing process.

The inner tube 121 and the cap 12 may be integrally formed, and the inner tube 121 and the cap 12 may be separately formed, wherein the integral forming may effectively improve the structural strength of the cap 12, and may simplify the forming process, but the integral forming has high requirements for the mold, and the cost of the former mold is high. Therefore, the present invention provides a structure in which the cap 12 and the inner tube 121 are assembled together after being separately molded, so as to simplify the production of the cap 12 and reduce the cost. Specifically, as shown in fig. 14 and 15, an annular groove is provided on the inner end surface of the pipe cap 12, an annular hook 1202 is provided on the end portion of the inner pipe 121, and the annular hook 1202 is engaged with the annular groove. Specifically, a rib structure may be disposed on the inner end surface of the pipe cap 12 to construct an annular groove, the annular groove may be configured to gradually contract inward in a direction away from the inner end surface of the pipe cap 12, or the radial dimension of the annular groove gradually decreases in a direction away from the inner end surface of the pipe cap 12, and a structure corresponding to the annular groove may be disposed on the inner shell, for example, the annular hook 1202 on the inner shell is disposed in a shape gradually expanding outward, and by the cooperation of the annular groove and the annular hook 1202, the stable connection between the inner tube 121 and the pipe cap 12 may be achieved, and the connection strength between the inner tube 121 and the pipe cap 12 is improved.

In addition, in the utility model, a plurality of reagent cavities can be arranged at intervals along the circumferential direction of the tube cap, and the plurality of reagent cavities are punctured in sequence by the puncturing structure through the rotation of the tube cap relative to the tube body.

As described above, a plurality of reagent chambers may be disposed in the tube cap of the present invention, wherein a sample storage solution, a lysis solution, a first cleaning solution, a second cleaning solution, and an air column may be disposed in the plurality of reagent chambers, and the sample storage solution, the lysis solution, the first cleaning solution, the second cleaning solution, and the air column are sequentially disposed to implement respective addition. The sample preserving fluid, the lysis fluid, the first cleaning fluid, the second cleaning fluid, the air column and the like are sequentially arranged in the tube cap along the puncture sequence of the puncture structure. For example, in the embodiment that a plurality of reagent chambeies set up along the axis direction interval of body, sample preservation liquid, lysate, first washing liquid, second washing liquid and air column set gradually in a plurality of reagent intracavity that can set up along body axis direction, moreover, the reagent chamber that is used for depositing sample preservation liquid is close to the opening of pipe cap to in the sample intracavity is put in with sample preservation liquid at first.

In the utility model, the end part of the sleeve can be constructed into the puncture structure, so that the pipe body is simplified.

In order to further facilitate the puncture of the reagent chamber 1201 by the sleeve 111, a plurality of notch grooves 1103 provided at intervals in the circumferential direction of the sleeve 111 may be provided at the end of the sleeve 111, as shown in fig. 14 and 21. In this way, during the process of puncturing the reagent chamber 1201 by the cannula 111, the side edge of the notch groove 1103 can provide a plurality of puncturing points for the cannula 111 to improve the puncturing efficiency, in addition, the tube cap 12 in the present invention can be configured in a form of being in threaded connection with the tube body 11, the puncturing of the reagent chamber 1201 by the cannula 111 is realized by rotating the nut, during this process, the rotation of the tube cap 12 enables the cannula 111 to rotatably puncture the reagent chamber 1201, and by providing the notch groove 1103, the force applied to the reagent chamber 1201 by the peripheral edge of the notch groove 1103 will have a good stress concentration phenomenon, so as to realize the rapid puncturing of the reagent chamber 1201.

In some embodiments of the utility model, as shown in fig. 14, the inner end surface of the cap 12 is provided with a sealing rib 1204, and the sealing rib 1204 is adapted to abut against the inner peripheral edge of the second end of the tube 11 so as to make the cap 12 and the tube 11 in sealing engagement. Through setting up sealing rib 1204, when tube cap 12 moved predetermined position along the axis direction of body 11, for example the tip of body 11 supports and leans on the interior terminal surface of tube cap 12, sealing rib 1204 will be with the interior peripheral edge laminating of body 11 to realize the sealed between tube cap 12 and the body 11, avoid the reagent in the reagent bottle 100 to spill, improve the precision of testing result, avoid extravagant.

In the present invention, the pipe cap 12 may move along the axial direction of the pipe body 11 along the linear direction, and certainly, the pipe cap 12 may also move relative to the pipe body 11 in other manners, preferably, in an embodiment of the present invention, the pipe cap 12 is in threaded connection with the pipe body 11, which not only facilitates stable connection between the pipe cap 12 and the pipe body 11, but also enables the pipe cap 12 to move relative to the pipe body 11 through thread transmission, and in addition, the thread transmission may enable the sleeve 111 to have a greater force to puncture the reagent chamber 1201, thereby improving structural stability and reagent dispensing efficiency. Referring to fig. 14 to 16, in some embodiments of the present invention, the pipe cap 12 is sleeved outside the pipe body 11, the inner circumferential surface of the pipe cap 12 is provided with a first coarse thread 1205, the outer circumferential surface of the pipe body 11 is provided with a second coarse thread 1104, and the first coarse thread 1205 is matched with the second coarse thread 1104. Through the cooperation of first coarse thread 1205 and second coarse thread 1104, stable connection of cap 12 and body 11 can be achieved.

The outer circumferential surface of the tube body 11 is further provided with fine threads which are engaged with the second coarse threads 1104, and the first coarse threads 1205 are in contact with the fine threads when the sleeve 111 pierces the reagent chamber 1201. Through the cooperation of first coarse thread 1205 and second coarse thread 1104, can realize the screw-thread fit of pipe cap 12 and body 11, and the fine thread that sets up on body 11 can strengthen the resistance to rotatory pipe cap 12, when carrying out manual rotation pipe cap 12, can give the appropriate feedback of user, reminds the user. Due to the arrangement position of the fine threads, the resistance borne by the tube cap 12 can be increased in the process that the sleeve 111 punctures the reagent cavity 1201, so that a user can be reminded of using the reagent cap.

In addition, as mentioned above, the plurality of reagent chambers 1201 are arranged in the cap 12 of the present invention, and the user can be prompted by setting the fine thread, and if the required reagent types are smaller than the actual number of the reagent chambers 1201, the user can find and control the reagent chambers in time.

That is, during the rotation of the cap 12 of the present invention, when the wall (such as the diaphragm 122) of the reagent chamber 1201 in the cap 12 starts to touch or is about to touch the sleeve 111, the first coarse threads 1205 will cooperate with the second coarse threads 1104 and the fine threads to provide timely feedback to the user, so that the user can know the working status of the reagent bottle 100 to make adjustments.

Alternatively, referring to fig. 16, 22-24, the first end of the tube 11 is provided with an inner end plate 112 and an outer end plate 113, a mold cavity is formed between the inner end plate 112 and the outer end plate 113, a first via hole 1106 is formed on the inner end plate 112, and the first via hole 1106 connects the sample cavity 1102 and the mold cavity. Through setting up inner end plate 112 and outer end plate 113, can conveniently realize the closure of body 11 first end, simplify the structure of die cavity moreover, make things convenient for the shaping of body 11, in addition, can place in the die cavity and draw membrane 118, draw membrane 118 can be by interior end cap and outer end cap centre gripping, improve reagent bottle 100's structural strength and stability.

Wherein the first via hole and the second via hole are suitable for being heat-sealed by a heat sealing device. Specifically, after the reagent bottle is inserted into the insertion opening, the reagent bottle may be inserted onto the inner end plate through the outer end plate by a heat-seal needle, and the first and second via holes on the inner end plate are closed.

Moreover, in order to facilitate heating, observation and the like of the reagent bottle, the reagent bottle can be locked through the positioning module in the embodiment, wherein the positioning module can be used for locking the tube body and the tube cap of the reagent bottle, and the tube cap can be driven to move relative to the tube body through the positioning module, so that the reagent can be put into the reagent bottle. For example, the cap is driven to rotate relative to the body by the positioning module.

Alternatively, referring to fig. 13 and 19, a slip-off preventing groove 1107 is provided on the outer circumferential surface of the first end of the pipe body 11, and the slip-off preventing groove 1107 is provided along the circumferential direction of the pipe body 11. The anti-drop groove 1107 may be an annular groove extending along the circumferential direction of the tube body 11, or may be a groove intermittently extending along the circumferential direction of the tube body 11, wherein when the tube body 11 is placed in the detection apparatus, the axial positioning of the tube body 11 may be realized by a corresponding structure on the detection apparatus, so as to prevent the reagent bottle 100 from dropping out of the detection apparatus.

In connection with the previous embodiment, the positioning module is adapted to cooperate with the anti-drop groove to position the reagent bottle along the axial direction of the insertion port, wherein the latch in the previous embodiment can be inserted into the anti-drop groove to also achieve the locking of the reagent bottle.

Further, a retaining projection or the like may be provided on the outer peripheral surface of the first end of the tube body 11, and the axial positioning of the reagent bottle 100 can be similarly achieved by the retaining projection or the like.

Optionally, with reference to fig. 19, a positioning groove 1108 may be further disposed on the outer peripheral surface of the first end of the pipe body 11, the positioning groove 1108 may extend in a direction parallel to the axis of the pipe body 11, and in the using process, a positioning rib may be disposed at a corresponding position of the detection device to achieve circumferential positioning of the pipe body 11.

Can realize the circumference location to the body through aforementioned orientation module, for example, with in aforementioned location muscle imbeds the constant head tank, realize the circumference locking of body.

Alternatively, as shown in fig. 13, a plurality of splines 1206 are provided on the outer circumferential surface of the cap 12, the splines 1206 are parallel to the axis of the tube 11, and the splines 1206 are spaced apart along the circumferential direction of the cap 12. The plurality of splines 1206 can increase the friction on the outer circumferential surface of the cap 12, and can improve the stable operation of the reagent bottle 100 when the cap 12 is manually or automatically driven to move in the axial direction of the tube 11.

The splines 1206 may be used to improve the stability of the manual or motorized rotation of the cap 12, for example, circumferential locking of the cap may be achieved by the positioning module cooperating with the splines, and then reagent may be dispensed by rotating the cap.

Wherein the splines 1206 on the outer circumferential surface of the cap 12 may be arranged to extend in a direction parallel to the axis of the tube 11.

Alternatively, as shown in fig. 13 and 19, the outer circumferential surface of the first end of the tube body 11 is provided with a non-slip rib 1109, the non-slip rib 1109 extends in a direction parallel to the axis of the tube body 11, and a plurality of non-slip ribs 1109 are provided at intervals in the circumferential direction of the tube body 11. The antiskid rib 1109 can play the effect of increasing 11 frictional forces of body, and in the use, when rotatory cap 12, need be to cap 12 and 11 application of force simultaneously of body, consequently, through set up tooth's socket 1206 on cap 12, set up antiskid rib 1109 on body 11, can make things convenient for cap 12 for the rotation of body 11.

Of course, the present invention may be provided with other structures to increase the frictional force between the outer peripheral surface of the cap 12 and the outer peripheral surface of the pipe body 11, for example, a groove-shaped, convex-shaped, or other anti-slip structure is provided on both the outer peripheral surface of the cap 12 and the outer peripheral surface of the pipe body 11.

In addition, the extraction cavity can be internally provided with a replaceable extraction film, the extraction film can be pre-placed in the extraction cavity for convenient use, the pollution of the external environment is avoided, the extraction cavity is internally provided with the extraction film, the waste liquid cavity is communicated with the inner side surface of the extraction film, and the sample cavity is communicated with the outer side surface of the extraction film.

As mentioned above, the cap 12 of the present invention is provided with the reagent chamber 1201, but in other embodiments of the present invention, a hollow chamber may be provided in the tube 11 for placing the reagent, or the reagent may be placed in both the tube 11 and the cap 12.

Referring to fig. 16, in some embodiments of the present invention, the tube body has a piston cavity 1110 and a piston rod 114 therein, the piston cavity 1110 communicates with the extraction cavity, and the inner end surface of the piston cavity 1110 is provided with a puncturing structure; cap 12 is adapted to press against plunger rod 114 to force reagent in plunger cavity 1110 into the mold cavity, wherein reagent may be placed in plunger cavity 1110, and when cap 12 is moved in a direction parallel to the axis of body 11, plunger rod 114 may be pressed by cap 12 to force reagent in plunger cavity 1110 into the mold cavity.

In addition, reagents can be arranged in the tube cap 12 and the tube body 11, so as to improve the application range of the reagent bottle 100 of the present invention, and in the actual use process, the reagents in the piston cavity 1110 can be added after the reagents in the tube cap 12 are added, that is, in the moving process of the tube cap 12, after the sleeve 111 punctures all the reagent cavities 1201 in the tube cap 12, the tube cap 12 drives the piston rod 114 to inject the reagents in the piston cavity 1110 into the mold cavity. Of course, the order of putting the reagents in the cap 12 and the tube 11 can be selected according to actual use.

Alternatively, as shown in fig. 16, a sealing ring 115 is disposed between the outer circumferential surface of the piston rod 114 and the inner circumferential surface of the piston cavity 1110, and the sealing ring 115 seals a gap between the piston rod 114 and the piston cavity 1110. The sealing ring 115 can seal the gap between the piston rod 114 and the piston cavity 1110, so that the reagent in the piston cavity 1110 can be prevented from flowing out, and the articles outside the piston cavity 1110 can be prevented from entering the piston cavity 1110, thereby improving the stability of the reagent bottle 100.

A first sealing groove is formed in the outer circumferential surface of the piston rod 114, the sealing ring 115 is embedded in the first sealing groove, and the sealing ring 115 protrudes out of the outer circumferential surface of the piston rod 114. So that the packing 115 can be stably mounted on the piston rod 114. In addition, a second sealing groove is formed in the inner circumferential surface of the piston chamber 1110, and the portion of the sealing ring 115 protruding out of the outer circumferential surface of the piston rod 114 is adapted to be embedded in the second sealing ring 115 and to slide out of the second sealing groove. In this way, before the piston rod 114 starts moving, stable sealing between the piston rod 114 and the inner circumferential surface of the piston chamber 1110 is achieved by the engagement of the first seal groove, the second seal groove, and the seal ring 115, and when the piston rod 114 is driven to move by the cap 12, the seal ring 115 can be slid out of the second seal groove, thereby achieving stable extension and contraction of the piston rod 114. Plunger rod 114 and plunger cavity 1110 are configured similar to a syringe to facilitate injection of reagents into the mold cavity.

Of course, in the present invention, the packing 115 may be fixed to the inner circumferential surface of the seal chamber, and the piston rod 114 may be movable relative to the packing 115.

Referring to fig. 14 and 16, in some embodiments of the present invention, an inner tube 121 is disposed in the cap 12, the inner tube 121 extends along the axis of the tube 11, and the cap 12 is adapted to drive the inner tube 121 against the piston rod 114. Therefore, the stability of the driving of the piston rod 114 can be improved, and in combination with the aforementioned embodiment, the plurality of reagent chambers 1201 are arranged in the inner tube 121, so that the inner tube 121 can press the driving piston rod 114 while constructing the plurality of reagent chambers 1201 in the tube cap 12, thereby optimizing and simplifying the structure of the reagent bottle 100.

Optionally, a plurality of reagent chambers 1201 are arranged in the inner tube 121 at intervals along the axis of the tube 11, and the inner tube 121 is adapted to be inserted into the tube 11 from the second end of the tube 11 so as to be suitable for the cannula 111 to sequentially puncture and communicate the plurality of reagent chambers 1201 and make the liquid reagent in the reagent chambers 1201 enter the sample chamber 1102.

As described above, with reference to fig. 13 to 24, the present invention provides a reagent bottle 100, wherein the sleeve 111 and the tube 11 may be in a concentric column shape, the hollow structure of the sleeve 111 may form the sample chamber 1102, and a partition structure may be disposed in the cavity structure between the sleeve 111 and the tube 11 to form the plunger chamber 1110, and the piston rod 114 is disposed and telescopically inserted into the plunger chamber 1110, and the sleeve 111 and the tube 11 may be in an integrally formed structure to form the partition structure of the plunger chamber 1110, and may also be integrally formed with the sleeve 111 and the tube 11. A second coarse thread 1104 and a fine thread are provided on the outer peripheral surface of the second end of the tubular body 11, the fine thread and the second coarse thread cooperating with each other. Pipe cap 12 rotationally cup joints on body 11, and has set up first thick screw 1205 on the inner peripheral surface of pipe cap 12, and first thick screw 1205 mutually supports with second thick screw 1104 in order to realize the screw drive between pipe cap 12 and the body 11, and the thin screw on the body 11 outer peripheral surface can provide the resistance for the rotation of pipe cap 12. The inner end surface of the pipe cap 12 is provided with a sealing rib 1204, and when the pipe body 11 is inserted into the top of the pipe cap 12, the periphery of the second end of the pipe body 11 can be matched with the sealing rib 1204 to improve the sealing effect. The inner tube 121 may be disposed in the tube cap 12, the inner tube 121 is in a bell mouth shape, a plurality of diaphragms 122 are disposed in the inner tube 121 and arranged at intervals, a plurality of reagent chambers 1201 are configured in the inner tube 121 by the plurality of diaphragms 122, and different reagents may be stored in the plurality of reagent chambers 1201. In use, a sample is placed in the sleeve 111, the cap 12 is placed on the tube 11, the cap 12 is rotated, the cap 12 is opposite to the central region of the diaphragm 122, the diaphragm 122 contacts the sleeve 111 with the rotation of the cap 12, the first coarse thread 1205 contacts the fine thread, the sleeve 111 is further rotated to pierce the diaphragm 122, and the reagent in the reagent chamber 1201 enters the sleeve 111 to contact and mix with the sample in the sample chamber 1102. With the cap 12 rotated, the plurality of reagent chambers 1201 may be pierced by the cannula 111, thereby completing the administration of the plurality of reagents.

Meanwhile, when the tube cap 12 moves to a preset position, the tube cap 12 will contact the piston rod 114, at this time, the piston rod 114 will provide a reverse acting force for the tube cap 12, so as to slow down the movement of the tube cap 12, so as to facilitate the sufficient mixing of the reagent and the sample, and avoid the influence of the simultaneous mixing of various reagents on the detection effect, and meanwhile, the piston cavity 1110 can also be provided with the reagent, so that when the tube cap 12 moves to a specific position, the tube cap 12 can drive the piston rod 114 to inject the reagent in the piston cavity 1110 into the mold cavity.

In the present invention, test paper, an extraction film 118, and the like may be placed in the cavity.

As mentioned above, the present invention describes the addition of reagents to the cannula 111, and different reagents may be added to the cannula 111 to accomplish the desired detection. Finally, the reagent and the sample added to the sleeve 111 are mixed and then allowed to enter the cavity. The entry and exit of reagents into and out of the mold cavity in some embodiments of the utility model is described below with reference to the accompanying drawings.

Referring to fig. 14, 16 and 22-24, in some embodiments of the present invention, a waste liquid chamber is formed between the sleeve 111 and the tube 11, and the mold cavity is communicated with the waste liquid chamber. Wherein, a sample cavity 1102 is formed in the sleeve 111, and a waste liquid cavity is formed outside the sleeve 111, in the using process, a reagent can be added into the sample cavity 1102, the reagent can enter the mold cavity through the sample cavity 1102, and the redundant reagent can flow back into the waste liquid cavity through the mold cavity, so as to improve the stability of the reagent bottle 100. Wherein, the extraction film 118 (for example, for nucleic acid extraction) can be arranged in the die cavity, the reagent and the sample can be conveniently stored in the die cavity through the extraction film 118, and the redundant part enters the waste liquid cavity, thereby avoiding waste.

In some embodiments of the present invention, a first end of tube 11 has an inner end plate 112 and an outer end plate 113, a mold cavity is defined between inner end plate 112 and outer end plate 113, a first via 1106 and a second via 1113 are defined in inner end plate 112, first via 1106 connects sample cavity 1102 and mold cavity, and second via 1113 connects waste cavity and mold cavity. Through setting up first via hole 1106 and second via hole 1113, can make things convenient for sample cavity 1102 and waste liquid chamber and die cavity intercommunication, realize that reagent circulates in sample cavity 1102, die cavity and waste liquid intracavity, accomplish work such as drawing of sample, realize the purpose of detecting the sample.

Optionally, as shown in fig. 22 and 23, a plurality of first via holes 1106 and a plurality of second via holes 1113 are provided on the inner end plate 112, the plurality of first via holes 1106 are spaced along the circumferential direction of the inner end plate 112, and the plurality of second via holes 1113 are spaced along the circumferential direction of the inner end plate 112, so that the efficiency of the reagent entering the mold cavity to contact the extraction film 118 in the mold cavity can be improved, and the backflow of the redundant reagent to the waste liquid cavity can be facilitated.

Further, the first via 1106 is spaced apart from the sample cavity 1102 by a distance greater than the distance between the second via 1113 and the sample cavity 1102. In this case, the reagent will flow back to the waste liquid chamber after passing through the extraction film 118 after entering the mold cavity, so as to avoid the reagent flowing back to the waste liquid chamber directly through the mold cavity, and provide sufficient reaction time between the reagent and the sample.

Referring to fig. 16 and 21, in some embodiments of the present invention, the first end of the tube 11 is provided with an annular plate 116, the inner periphery of the annular plate 116 is connected to the sleeve 111, the outer periphery of the annular plate 116 is connected to the tube 11, the outer end surface of the annular plate 116 is provided with a plurality of first guide grooves 1119, the plurality of first guide grooves 1119 are all communicated with the sample chamber 1102 and extend in a divergent manner in a direction away from the sample chamber 1102, and the first guide grooves 1119 are communicated with the first through hole 1106. Therefore, the time and the distance for the reagent to flow in the process of entering the die cavity and being discharged from the die cavity can be increased, more reaction time is provided for the reagent and the sample, and the extraction effect is enhanced.

Optionally, with reference to fig. 16 and 21, a third through hole 1114 is provided on the annular plate 116, the third through hole 1114 is spaced apart from the first guide groove 1119, the third through hole 1114 is opposite to the second through hole 1113, and the second through hole 1113 and the third through hole 1114 cooperate to connect the mold cavity and the waste liquid cavity. Make things convenient for the intercommunication in die cavity and waste liquid chamber, avoid the too much too big pressure that produces of reagent in the die cavity to subsequent other reagents can enter into the die cavity fast in.

Optionally, as shown in fig. 23, the outer side surface of the inner end plate 112 is provided with a receiving groove 1115, the outer end plate 113 covers the receiving groove 1115 to form a mold cavity, the bottom surface of the receiving groove 1115 is provided with criss-cross flow grooves 1116, and the second via holes 1113 are connected to the flow grooves 1116. The extraction film 118, etc., may be conveniently placed within the mold cavity and further increase the coverage of reagents within the mold cavity to facilitate extraction of the extraction film 118.

As can be seen from fig. 23, the accommodating groove 1115 is circular groove-shaped, the bottom surface of the accommodating groove is provided with a circular circulation groove 1116, the circulation groove 1116 is internally provided with a plurality of annular ribs, the annular ribs are arranged from inside to outside at intervals, and the annular ribs are communicated with a plurality of radial grooves, so that the reagent can uniformly flow into the extraction film in the mold cavity.

As shown in fig. 24, in some embodiments of the present invention, the inner side surface of the outer end plate 113 is provided with a central groove 1117 and a second guiding groove 1118, the central groove 1117 forms a part of the mold cavity, the second guiding groove 1118 communicates with the central groove 1117 and extends in a diverging manner in a direction away from the central groove 1117, and the second guiding groove 1118 communicates with the first via hole 1106.

The reagent bottle 100 according to the embodiment of the present invention has a structure in which the reagent is partitioned by the multi-layered membrane 122, and is suitable for a detection reaction in which a detection reagent needs to be added in several times. Such as nucleic acid detection of the novel coronavirus.

The specific technical scheme can have a plurality of different modes: (1) in the multilayer design shown in fig. 14, the cap 12 is rotated to sample the sample by puncturing step by step; (2) different reagents are sealed on the same plane, the puncture structure is designed at the edge position, the first reagent is punctured by downward displacement of the tube cap 12, and the other reagent cavities 1201 are punctured to puncture the reagent separation membranes 122 one by one in a mode of rotating the tube cap 12.

The body of the reagent bottle 100 of the present invention may be provided with a rotary scale to indicate the type of reagent to be added.

The utility model stores the multi-component reagent into a reaction integrated tube, the reaction reagents are stored in a container, and each component is divided (can be a film or a sheet) by a 'dividing sheet' physical method, so that the cross is avoided. The position of each component reagent in the tube is set according to the experimental needs. The amount of the reagent components is set according to the experimental requirements. The sample can be placed into the sleeve 111 after the cover is opened, and the tube cover is screwed on after the sample is placed. When the device is used, the displacement is generated by the rotation or pressing of the screw threads, the sleeve 111 punctures the diaphragms 122 of reagents with different components one by one, so that the reagents with different components flow into the sleeve 111 in sequence, and enter the die cavity for reaction after passing through a sample. There may be a separate microchannel into the mold cavity without the need for reagent components to flow through the sample. The mold cavity is connected with the sample cavity 1102 and the waste liquid cavity through micro-pipes. The micro-pipe has a plurality of groups (a plurality of first via holes 1106 and a plurality of second via holes 1113), can realize large particle filtration, prevents a certain jam.

The extraction film 118 can be arranged in the die cavity, the extraction film 118 can be a thin sheet (the height of the optimized scheme is 0.2 mm), the heating area is large, the heat conduction is uniform, and the PCR reaction time is shortened. For the particle sample, the focal plane of the microscopic image was stable. The tube cap 12 is designed with steps corresponding to the liquid height of each reagent component, and when the tube cap reaches the position, the resistance is increased, and the tube cap plays a role in position reminding during manual operation. The position lines of the reagent components can be printed on the outer peripheral surface of the tube body 11, and the position reminding effect is achieved during manual operation.

The reagent bottle 100 of the present invention may be operated manually or by a machine.

The tube body 11 and the tube cap 12 in the utility model are designed to be sealed, the tube body 11 and the tube cap 12 are sealed after the last component reagent flows into the sleeve 111, and all reagents are sealed in the tube to avoid leakage. The waste liquid cavity can be filled with water absorbing materials (filter paper or sponge and the like), and waste liquid enters the waste liquid cavity during operation and is absorbed by the water absorbing materials to prevent the waste liquid from flowing back into the micro-pipeline.

The utility model does not need any auxiliary tool and realizes nucleic acid extraction.

The PCR reagent bottle 100 of the present invention is mainly characterized by the structure of the flow channel, the small chamber for dispensing the enzyme, the eluent and the PCR reaction chamber. Is suitable for directly adding extracted nucleic acid. The subpackaged enzyme in each reagent cavity 1201 of the utility model adopts a freeze-drying mode, and each reagent cavity 1201 of the utility model can be respectively provided with: sample preserving fluid, lysate, cleaning fluid 1, cleaning fluid 2, eluent and enzyme reaction system. The cavity in the present invention can be used as a PCR chamber, and the reaction solution can enter the cavity from the sample cavity 1102 or enter the waste solution cavity from the cavity.

The reaction can be promoted by heating the extraction membrane in the mold cavity at different temperatures, and detection can be achieved by observing the extraction membrane with different filters.

In addition, the present invention also provides a detection system, which comprises: the reagent bottle is the reagent bottle according to the embodiment, the positioning module is provided with an insertion opening, the first end of the reagent bottle is suitable for being inserted into the insertion opening and is positioned by the positioning module, the heating module is used for heating the extraction cavity, and the shooting module is opposite to the end face of the first end of the reagent bottle and is suitable for obtaining a fluorescence quantitative image.

According to the detection system provided by the embodiment of the utility model, the addition of various reagents can be realized under the condition of a closed tube, the reaction, nucleic acid extraction and other work can be carried out in the closed tube body after various actual addition, and the reagent bottle can be used as a reaction cavity, so that the extraction membrane is not required to be taken out in the process of obtaining the fluorescence quantitative image, and the acquisition and reaction are directly carried out, thereby effectively improving the detection precision, avoiding the pollution to the external environment and avoiding the influence of the external environment on the detection process.

Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.

Claims

1. A reagent bottle, comprising:

the device comprises a pipe body, wherein a sleeve is arranged in the pipe body, a sample cavity is constructed in the sleeve, a waste liquid cavity is constructed between the sleeve and the pipe body, an extraction cavity is arranged at a first end of the pipe body, the waste liquid cavity and the sample cavity are both communicated with the extraction cavity through a micro-channel, and a puncture structure is arranged in the pipe body;

the pipe cap covers the second end of the pipe body, a closed reagent cavity is arranged in the pipe cap, and the pipe cap is movably connected with the pipe body so as to be suitable for the puncture structure to puncture the reagent cavity and enable the reagent cavity to be communicated with the sleeve for closed pipe extraction.

2. The reagent bottle of claim 1, wherein a plurality of reagent chambers are spaced within the cap, the plurality of reagent chambers being adapted to be sequentially pierced by the piercing structure and sequentially communicate with the cannula.

3. The reagent bottle according to claim 2, wherein the cap is movably connected to the tube body in an axial direction of the tube body, and the plurality of reagent chambers are provided at intervals in an axial direction of the cap.

4. The reagent bottle of claim 3, wherein an inner tube is disposed in the tube cap, the inner tube extends along an axis of the tube body, an end of the inner tube away from the sleeve is closed and is open toward an end of the sleeve, a plurality of diaphragms are disposed in the inner tube, the plurality of diaphragms are spaced along the axis of the tube body, and the reagent chamber is formed between each two adjacent diaphragms and between each diaphragm and the closed end of the inner tube.

5. The reagent bottle according to claim 4, wherein the diaphragm includes a hard film layer and a flexible film layer, the hard film layer is connected to a periphery of the flexible film layer, the hard film layer and the flexible film layer are connected in a flat plate shape, the flexible film layer is opposite to the sleeve in an axial direction of the pipe body, and a thickness of the flexible film layer is lower than a thickness of the hard film layer.

6. The reagent bottle according to claim 5,

the flexible film layer and the hard film layer are configured into a concave structure with an opening facing the sleeve; and/or

The surface of the flexible film layer is provided with a plurality of strip-shaped grooves which are connected at one position and are constructed into a divergent shape.

7. The reagent bottle as claimed in claim 4, wherein the inner tube is formed in a tapered tubular shape having a radial dimension gradually decreasing in a direction away from the sleeve, and a plurality of steps are formed on an inner circumferential surface of the inner tube, and the diaphragm is provided in the sleeve and supported on the steps on the inner circumferential surface of the inner tube.

8. The reagent bottle as claimed in claim 4, wherein the inner end surface of the cap is provided with a ring-shaped slot, and the end of the inner tube is provided with a ring-shaped hook, and the ring-shaped hook is engaged with the ring-shaped slot.

9. A reagent bottle according to claim 2, wherein the cap is rotatably connected to the body, and a plurality of the reagent chambers are provided at intervals along a circumferential direction of the cap.

10. The reagent bottle of any one of claims 2 to 9, wherein a sample preservation solution, a lysis solution, a first cleaning solution, a second cleaning solution and an air column are sequentially disposed in the cap along the puncture sequence of the puncture structure.

11. A reagent bottle according to any of claims 1 to 9, wherein the end of the sleeve constitutes the piercing structure, the end of the sleeve being provided with a plurality of notched grooves arranged at intervals in the circumferential direction of the sleeve.

12. A reagent bottle according to any of claims 1 to 9, wherein the cap is threadedly engaged with the body.

13. The reagent bottle of claim 12, wherein the cap is sleeved outside the tube body, a first coarse thread is provided on an inner circumferential surface of the cap, a second coarse thread is provided on an outer circumferential surface of the tube body, the first coarse thread and the second coarse thread are engaged,

and fine threads are further arranged on the outer peripheral surface of the tube body, are matched with the second coarse threads, and are in contact with the first coarse threads when the sleeve pierces the reagent cavity.

14. A reagent bottle as claimed in claim 1, wherein the inner end surface of the cap is provided with a sealing rib adapted to abut against the inner peripheral edge of the second end of the tube body to sealingly engage the cap with the tube body.

15. The reagent bottle according to claim 1,

the anti-drop groove is arranged on the peripheral surface of the first end of the pipe body and arranged along the circumferential direction of the pipe body; and/or

A plurality of tooth grooves are formed in the outer peripheral surface of the pipe cap, the tooth grooves are parallel to the axis of the pipe body, and the tooth grooves are arranged at intervals along the circumferential direction of the pipe cap; and/or

The anti-skidding ribs are arranged on the outer peripheral surface of the first end of the pipe body, extend in the direction parallel to the axis of the pipe body and are arranged in a plurality at intervals along the circumferential direction of the pipe body.

16. The reagent bottle of claim 1, wherein an extraction membrane is disposed in the extraction chamber, the waste fluid chamber communicates with an inner side surface of the extraction membrane, and the sample chamber communicates with an outer side surface of the extraction membrane.

17. A detection system, characterized in that the detection system comprises: a positioning module, a heating module, a photographing module, and a reagent bottle according to any one of claims 1 to 16,

the positioning module is provided with an insertion opening, the first end of the reagent bottle is suitable for being inserted into the insertion opening and is positioned by the positioning module, the heating module is used for heating the extraction cavity, and the shooting module is opposite to the end face of the first end of the reagent bottle and is suitable for acquiring a fluorescence quantitative image.