CN214991576U

CN214991576U - Sample detection device

Info

Publication number: CN214991576U
Application number: CN202121033067.7U
Authority: CN
Inventors: 阳卫超; 侯卫峰
Original assignee: Guangzhou Dina Biotechnology Co ltd
Current assignee: Guangzhou Dina Biotechnology Co ltd
Priority date: 2021-04-08
Filing date: 2021-05-14
Publication date: 2021-12-03
Anticipated expiration: 2031-05-14
Also published as: CN113512488A

Abstract

The utility model relates to a sample detection device, including reagent pipe, tube cap, reaction vessel and magnetic sleeve. One end of the reagent tube is provided with an opening, and the reagent tube is at least internally provided with a cracking cavity and an elution cavity. The tube cover is arranged at the opening end of the reagent tube and is rotatably connected with the reagent tube. The tube cover is provided with a first sample adding hole and a through hole. The magnetic sleeve is inserted in the penetrating hole and can move in the direction close to or far away from the reagent tube, and the magnetic sleeve is used for matching with the magnetic rod to attract and release magnetism of the magnetic beads. The reaction container is provided with a reaction cavity, and liquid in the elution cavity can flow into the reaction cavity. Therefore, the pretreatment of the sample to be detected, the separation and extraction of the target substance and the detection are integrated in the reagent tube, seamless butt joint in the whole process is realized, and the use is convenient. In addition, the sample to be detected is prepared and detected in a closed space, so that the pollution probability of the sample to be detected is reduced, the detection reliability is ensured, and the biological hazard is reduced.

Description

Sample detection device

Technical Field

The utility model relates to a biological detection technical field especially relates to a sample detection device.

Background

In medical inspection, molecular detection is mainly applied to the aspects of detection of the infection of pathogenic microorganisms and the like. The mainstream technology of molecular detection is fluorescence quantitative PCR (polymerase chain reaction), and due to the characteristics of exponential amplification template of PCR technology, aerosol pollution, complex operation and other factors become factors that limit the further application of fluorescence quantitative PCR technology in clinical medicine.

The conventional experiment operation is mostly carried out in an open experiment environment, a sample to be tested can be directly contacted with the outside through the air, but samples which are harmful to operators, easy to be interfered by the outside or generate interference to the outside, such as biological samples containing fulminant infectious diseases, often exist.

SUMMERY OF THE UTILITY MODEL

Therefore, there is a need for a sample detection device that integrates the pretreatment of the sample to be detected, the separation, extraction, and detection of the target substance, realizes seamless docking of the whole detection process, and reduces biohazard.

A sample testing device comprising:

the device comprises a reagent tube, a reaction tube and a reaction tube, wherein one end of the reagent tube is provided with an opening, the reagent tube is at least provided with a cracking cavity and an elution cavity, and magnetic beads are arranged in the reagent tube;

the tube cover is arranged at the opening end of the reagent tube and is rotatably connected with the reagent tube, the tube cover is provided with a through hole and a first sample adding hole for adding a sample to be detected into the cracking cavity, and the reagent tube is rotated to enable the through hole to at least correspond to the cracking cavity and the elution cavity respectively;

the magnetic sleeve is inserted into the penetrating hole and can move in a direction close to or far away from the reagent tube, and the magnetic sleeve is used for matching with a magnetic rod to attract and release magnetism of the magnetic beads; and

the reaction vessel is provided with a reaction cavity, and liquid in the elution cavity can flow into the reaction cavity.

In one embodiment, a sealing element is arranged in the through-insertion hole, and the magnetic sleeve can penetrate through the sealing element and is in sealing fit with the tube cover through the sealing element.

In one embodiment, the reaction container comprises a reaction tray, the reaction tray is provided with a main flow channel and at least two reaction chambers, the at least two reaction chambers are respectively communicated with the main flow channel, and the liquid in the elution chamber can respectively flow into the at least two reaction chambers through the main flow channel.

In one embodiment, the main flow channel is provided with at least two first channel sections, and the first channel sections extend towards the direction far away from the center of the reaction disk, are bent and then extend towards the direction close to the center of the reaction disk; the bending position of the first channel section is provided with a first branch flow channel, the first branch flow channel is located on one side, away from the center of the reaction disc, of the main flow channel, and the reaction chamber is communicated with the main flow channel through the first branch flow channel.

In one embodiment, the main runner is an arc runner, the arc runner extends along the circumference of the reaction disc, one side of the arc runner away from the center of the reaction disc is provided with at least two first branch runners, the at least two first branch runners are arranged along the extending direction of the arc runner, and the reaction chamber is communicated with the arc runner through the first branch runners.

In one embodiment, the reaction chamber comprises at least two chambers, and the at least two chambers are arranged on one side of the main flow channel away from the center of the reaction disk and are arranged at intervals along the radial direction of the reaction disk; the reaction disc is also provided with a second branch flow channel, and the two adjacent cavities are communicated through the second branch flow channel.

In one embodiment, the tube cover includes a first sealing cover and a rotating cover, the rotating cover is rotatably mounted at the open end of the reagent tube, the first sample adding hole is formed in the rotating cover, and the first sealing cover is used for sealing the first sample adding hole.

In one embodiment, the second sealing cover is provided with a hollow guiding column, the guiding column extends from the edge of the second sample adding hole to the direction close to the tube cover, and the guiding column penetrates through the first sample adding hole.

In one embodiment, the second sealing cover is provided with at least two puncturing parts which are respectively arranged in one-to-one correspondence with the cracking cavity and the elution cavity; the puncture part is arranged towards the inner recess of the reagent tube.

In one embodiment, the second sealing cover is at least one of a plastic cover and an aluminum film cover.

In one embodiment, a sample cavity, a magnetic bead cavity and a washing cavity are further arranged in the reagent tube, and the sample cavity is arranged in the middle of the reagent tube and is communicated with the lysis cavity; the lysis cavity, the magnetic bead cavity, the washing cavity and the elution cavity are arranged around the sample cavity.

In one embodiment, the first end of the magnetic sleeve is sealed, the second end of the magnetic sleeve is provided with an opening, and the magnetic rod can be inserted into the magnetic sleeve from the opening and can also be extracted from the magnetic sleeve; the first end of the magnetic sleeve is provided with a puncture part.

In one embodiment, the bottom of the lysis and elution chambers are hollow cylinders.

When the sample detection device is used for detecting a sample, the sample to be detected is added into the cracking cavity from the first sample adding hole, and the sample detection device is placed into the automatic operation instrument according to the positioning structure. Starting the automatic operation instrument, the automatic operation instrument drives the magnetic sleeve to be inserted into the cracking cavity, and the magnetic sleeve moves or rotates in the cracking cavity to uniformly mix the sample to be detected and the cracking solution, so that the cells of the sample to be detected are cracked, and the target detection object is released. After the sample to be detected releases the target detection object, the automatic operation instrument drives the magnetic sleeve to move upwards to the upper part of the reagent tube. The reagent tube is rotated to position the magnetic sleeve over the magnetic beads. The automatic operation instrument drives the magnetic sleeve and the magnetic rod to move downwards into the reagent tube, and magnetic beads are adsorbed under the matching of the magnetic sleeve and the magnetic rod. The automatic operation instrument drives the magnetic sleeve and the magnetic bar which are adsorbed with the magnetic beads to move upwards to the upper part of the reagent tube, and the reagent tube is rotated to ensure that the magnetic sleeve is positioned right above the cracking cavity. The automatic operation instrument drives the magnetic sleeve adsorbed with the magnetic beads to move downwards to the cracking cavity, the magnetic beads are released in the cracking cavity, and the target detection object of the sample to be detected is adsorbed by the magnetic beads. And transferring the magnetic beads adsorbed with the target detection objects to an elution cavity according to the method, and eluting the magnetic beads to obtain an eluent. And then the eluent is introduced into the reaction cavity, so that the target detection object in the eluent can react with the reactant in the reaction cavity, and the detection of the sample to be detected is realized. Therefore, through the matching of the reagent tube, the tube cover, the reaction container and the magnetic sleeve, the pretreatment of the sample to be detected, the separation and extraction of the target substance and the detection are integrated in the reagent tube, the seamless butt joint of the whole process is realized, and the use is convenient. In addition, the sample to be detected can be prepared and detected in a closed space, the pollution probability of the sample to be detected is reduced, the extraction and detection accuracy of the sample to be detected is improved, the reliability of a detection result is ensured, and meanwhile, the biological hazard can be reduced.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this application, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification.

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic structural diagram of a sample detection device according to an embodiment of the present invention;

FIG. 2 is an exploded view of the sample testing device shown in FIG. 1;

FIG. 3 is a schematic view of a reaction tray of the sample detection device shown in FIG. 1;

FIG. 4 is a plan view of a reagent vessel in the sample detection device shown in FIG. 1.

The reference numbers illustrate: 10. a reagent tube; 11. a sample chamber; 12. a lysis chamber; 13. a washing chamber; 14. an elution chamber; 15. a magnetic bead cavity; 20. a tube cover; 21. a rotating cover; 211. a first well; 212. inserting holes; 213. a seal member; 22. a first sealing cover; 30. a second sealing cover; 31. a second well; 32. a puncture section; 33. a guide post; 40. a reaction vessel; 41. a main flow channel; 411. a first channel segment; 412. a second channel segment; 42. a first branch flow channel; 43. a second branch flow channel; 44. a reaction chamber; 45. a sample inlet; 46. a sample outlet; 50. a magnetic sleeve; 51. a piercing portion.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, as those skilled in the art will be able to make similar modifications without departing from the spirit and scope of the present invention.

Referring to fig. 1 and 2, fig. 1 is a schematic structural diagram of a sample detection device according to an embodiment of the present invention, and fig. 2 is an exploded view of the sample detection device shown in fig. 1. An embodiment of the present invention provides a sample detection device, which comprises a reagent tube 10, a tube cap 20, a reaction vessel 40 and a magnetic sleeve 50. An opening is formed in one end of the reagent tube 10, at least a lysis cavity 12 and an elution cavity 14 are formed in the reagent tube 10, and magnetic beads are arranged in the reagent tube 10. The tube cover 20 is installed at the open end of the reagent tube 10 and is rotatably connected to the reagent tube 10. The tube cover 20 is provided with a first sample adding hole 211, and a sample to be detected can be added into the cracking cavity 12 through the first sample adding hole 211. The tube cover 20 is further provided with a through hole 212, and the through hole 212 can at least correspond to the lysis chamber 12 and the elution chamber 14. The magnetic sleeve 50 is inserted into the through hole 212 and can move in a direction close to or away from the reagent tube 10, and the magnetic sleeve 50 is used for matching with a magnetic rod to attract and release magnetism of the magnetic beads. The reaction container 40 is provided with a reaction cavity 44, and the liquid in the elution cavity 14 can flow into the reaction cavity 44.

Specifically, the lysis chamber 12 is filled with lysis solution, and the elution chamber 14 is filled with eluent.

In the sample detection device, when the sample is detected, the sample to be detected is added into the lysis chamber 12 from the first sample adding hole 211, and the sample detection device is placed into the automatic operation instrument according to the positioning structure. Starting the automatic operation instrument, the automatic operation instrument drives the magnetic sleeve 50 to be inserted into the cracking cavity 12, and the magnetic sleeve 50 moves or rotates in the cracking cavity 12 to uniformly mix the sample to be detected and the lysate, so that the cells of the sample to be detected are cracked, and the target detection object is released. After the sample to be tested releases the target detection object, the automatic operation instrument drives the magnetic sleeve 50 to move upwards to the upper part of the reagent tube 10. The reagent tube 10 is rotated so that the magnetic sleeve 50 is positioned directly above the magnetic beads. The automatic operation instrument drives the magnetic sleeve 50 and the magnetic rod to move downwards into the reagent tube 10, and magnetic beads are adsorbed under the matching of the magnetic sleeve 50 and the magnetic rod. The automatic operation instrument drives the magnetic sleeve 50 adsorbed with the magnetic beads and the magnetic bar to move upwards to the upper part of the reagent tube 10, and the reagent tube 10 is rotated to ensure that the magnetic sleeve 50 is positioned right above the cracking cavity 12. The automatic operation instrument drives the magnetic sleeve 50 adsorbed with the magnetic beads to move downwards into the cracking cavity 12, the magnetic beads are released in the cracking cavity 12, and the target detection object of the sample to be detected is adsorbed by the magnetic beads. According to the method, the magnetic beads with the target detection objects adsorbed thereon are transferred and released into the elution cavity 14, and the magnetic beads are eluted to obtain the eluent. Then the eluent is introduced into the reaction cavity 44, so that the target detection object in the eluent can react with the reactant in the reaction cavity 44, and the detection of the sample to be detected is realized. Thus, through the cooperation of the reagent tube 10, the tube cap 20, the reaction vessel 40 and the magnetic sleeve 50, the pretreatment of the sample to be detected, the separation and extraction of the target substance and the detection are integrated in the reagent tube 10, the seamless butt joint of the whole process is realized, and the use is convenient. In addition, the sample to be detected can be prepared and detected in a closed space, the pollution probability of the sample to be detected is reduced, the extraction and detection accuracy of the sample to be detected is improved, the reliability of a detection result is ensured, and meanwhile, the biological hazard can be reduced.

In one embodiment, referring to fig. 1 and 2, a sealing member 213 is disposed in the insertion hole 212, and the magnetic sleeve 50 can pass through the sealing member 213 and be in sealing engagement with the tube cover 20 through the sealing member 213. Therefore, gaps between the magnetic sleeve 50 and the tube cover 20 in the movement process are effectively avoided, the probability of pollution of a sample to be detected is reduced, the reliability of a detection result is ensured, and meanwhile, the biological hazard can be reduced.

Optionally, the sealing member 213 includes a sealing ring, an outer surface of which is in close contact with a hole wall of the penetration hole 212, and an inner side of which is in close contact with an outer surface of the magnetic sleeve 50. Of course, in other embodiments, the sealing member 213 may be other members with the same function, and is not limited thereto.

In one embodiment, referring to fig. 2 and 3, the reaction vessel 40 includes a reaction tray. The reaction tray is provided with a main flow channel 41 and at least two reaction chambers 44, the at least two reaction chambers 44 are respectively communicated with the main flow channel 41, and the liquid in the elution chamber 14 can respectively flow into the at least two reaction chambers 44 through the main flow channel 41. Alternatively, the reaction tray is mounted to the bottom of the reagent vessel 10, and at least two reaction chambers 44 have different reagents stored therein. Thus, the eluent in the elution chamber 14 is released, and the eluent containing the target detection object flows into the main flow channel 41; the reaction disk is rotated to respectively introduce the eluents in the main channel 41 into the at least two reaction chambers 44, and react with different reaction reagents in the at least two reaction chambers 44, so as to realize multiple detection of the sample to be detected.

Of course, in other embodiments, the reaction vessel 40 may also include a reaction tube that is mounted to the bottom of the elution chamber 14. When the reaction detection is needed, the bottom of the elution cavity 14 is punctured, so that the eluent can flow into the reaction tube to react with the reactant, and the detection of the sample to be detected is realized.

Specifically, the reaction tray includes a base plate and a cover plate covering the base plate, and the main flow channel 41 and the at least two reaction chambers 44 are disposed on one side of the base plate facing the cover plate. Therefore, the main flow channel 41, the reaction chamber 44 and the like are ensured to be in a closed environment, the sample to be detected is ensured to be detected in a closed space, the pollution probability of the sample to be detected is reduced, the reliability of a detection result is ensured, and meanwhile, the biohazard is reduced.

In one embodiment, referring to fig. 2 and 3, the main flow channel 41 is provided with at least two first channel segments 411, and the first channel segments 411 extend away from the center of the reaction tray and are bent to extend toward the center of the reaction tray. The first channel segment 411 is provided with a first branch flow channel 42 at a bent position, the first branch flow channel 42 is located on one side of the main flow channel 41 away from the center of the reaction disk, and the reaction chamber 44 is communicated with the main flow channel 41 through the first branch flow channel 42. Optionally, first channel section 411 is U-shaped or V-shaped. In this way, by providing the first channel segment 411, a steady flow effect can be achieved, and the eluent can be better stored in the main channel 41. In addition, the reaction chamber 44 is disposed on one side of the main channel 41 away from the center of the reaction tray and is communicated with the main channel 41 through the first branch channel 42, which is beneficial to quickly introduce the eluent into the reaction chamber 44 for reaction detection during centrifugation.

Further, referring to fig. 3, the main flow channel 41 is further provided with a second channel section 412, and the second channel section 412 extends from the first channel section 411 to a direction close to the center of the reaction tray and bends to extend to a direction away from the center of the reaction tray so as to communicate with the adjacent first channel section 411. Thus, the flow stabilizing function can be achieved, and the eluent can be better stored in the main flow channel 41, so that the eluent can be centrifugally guided into the reaction cavity 44.

Of course, in other embodiments, the main flow channel 41 may also be an arc-shaped flow channel, and the arc-shaped flow channel extends along the circumferential direction of the reaction disk. One side of the arc-shaped flow channel far away from the center of the reaction disc is provided with at least two first branch flow channels 42, and the at least two first branch flow channels 42 are arranged along the extending direction of the arc-shaped flow channel. The reaction chamber 44 communicates with the arc flow path through the first branch flow path 42.

In one embodiment, referring to fig. 3, the reaction chamber 44 includes at least two chambers, and at least two chambers are disposed on one side of the main flow channel 41 away from the center of the reaction disk and are spaced apart from each other in the radial direction of the reaction disk. The reaction disc is also provided with a second branch flow channel 43, and two adjacent cavities are communicated through the second branch flow channel 43. So, can deposit the reactant in the cavity that is close to sprue 41, prevent to bubble at the in-process that the reaction detected, also can guarantee simultaneously that eluant and reactant can more abundant reaction.

Specifically, in the present embodiment, the reaction chamber 44 includes two cavities, the two cavities are disposed on one side of the main flow channel 41 away from the center of the reaction disk, and the two cavities are spaced apart from each other in the radial direction of the reaction disk. The volume of the chamber adjacent to the main flow channel 41 is smaller than the volume of the other chamber, and the reactant is pre-loaded in the chamber adjacent to the main flow channel 41.

In one embodiment, referring to fig. 2 and 3, the reaction tray is further provided with a sample inlet 45 and a sample outlet 46, and the sample inlet 45 is arranged corresponding to the elution cavity 14. The sample inlet 45 and the sample outlet 46 are respectively disposed at two ends of the main flow channel 41 and are respectively communicated with the main flow channel 41. The elution cavity 14 comprises a first state and a second state, and in the first state, the elution cavity 14 is sealed with the sample inlet 45; in the second state, the elution chamber 14 is communicated with the sample inlet 45. Thus, after the magnetic beads are eluted to obtain the eluent, the sealing state between the elution cavity 14 and the sample inlet 45 is destroyed, so that the elution cavity 14 is communicated with the sample inlet 45, and the eluent can flow into the sample inlet 45; the eluent in the sample inlet 45 flows along the main channel 41 to the sample outlet 46. Then, the reaction disk is rotated to make the eluent in the main channel 41 flow into each reaction chamber 44 rapidly for reaction detection.

Optionally, the walls of the reaction chamber 44 can be punctured to allow the liquid in the elution chamber 14 to flow into the sample inlet 45. Thus, when the eluent needs to be subjected to reaction detection, the magnetic sleeve 50 is adopted to puncture the wall of the reaction cavity 44, so that the eluent flows into the sample inlet 45. Or, the reaction chamber 44 is provided with a liquid outlet hole, and the liquid outlet hole can be communicated with the sample inlet 45 through a liquid outlet valve. Therefore, when the eluent needs to be subjected to reaction detection, the liquid outlet valve is opened manually or through a control program, so that the eluent in the elution cavity 14 can flow into the sample inlet 45 through the liquid outlet valve.

In one embodiment, referring to fig. 1 and 2, the tube cover 20 includes a first sealing cover 22 and a rotating cover 21, and the rotating cover 21 is rotatably mounted to the open end of the reagent tube 10. The first sample application hole 211 is formed in the rotary cover 21, and the first seal cover 22 can seal the first sample application hole 211. When sample is added, the first sealing cover 22 is opened to expose the first sample adding hole 211, and the sample to be measured is added into the lysis chamber 12 from the first sample adding hole 211. After the sample is added, the first sealing cover 22 is sealed in the first sample adding hole 211, so that the sample to be detected is prepared and detected in a closed space, the probability of pollution of the sample to be detected is reduced, the accuracy of extraction and detection of the sample to be detected is improved, the reliability of a detection result is ensured, and meanwhile, the biohazard can be reduced.

In one embodiment, referring to fig. 2, the tube cover 20 further includes a second sealing cover 30, and the second sealing cover 30 is disposed at the opening of the reagent tube 10. The second sealing cover 30 is provided with a second sampling hole 31, and the second sampling hole 31 corresponds to the first sampling hole 211. When sample detection is required, a sample to be detected can be added into the reagent chamber through the first sample addition hole 211 and the second sample addition hole 31. Due to the fact that the second sealing cover 30 is arranged at the opening, the second sealing cover 30 is in sealing fit with the reagent tube 10, and therefore liquid in the reagent cavity can be limited in the reagent cavity through the second sealing cover 30.

Further, referring to fig. 2, the second sealing cover 30 is provided with a hollow guiding column 33, the guiding column 33 extends from the edge of the second sampling hole 31 to a direction close to the rotating cover 21, and the guiding column 33 penetrates through the first sampling hole 211. Specifically, the guide pillar 33 extends from the edge of the second well 31 in a direction away from the reagent tube 10, and the first well 211 is fitted to the guide pillar 33. In this manner, by providing the guide post 33, it is convenient to quickly fit the rotary cap 21 over the second sealing cap 30 and to make the first loading hole 211 correspond to the second loading hole 31. Meanwhile, in the process of rotating the reagent tube 10, the guide post 33 plays a role in guiding, so that the rotary cover 21 makes relative rotary motion with the reagent tube 10 around the guide post 33.

Further, referring to fig. 2, the second sealing cover 30 is provided with at least two piercing parts 32, and at least two piercing parts 32 are respectively arranged in one-to-one correspondence with the lysis chamber 12 and the elution chamber 14. After the sample to be tested is added into the lysis chamber 12, the sample testing device is placed into the automatic operation instrument according to the positioning structure. The magnetic sleeve 50 is inserted into the insertion hole 212 and positioned above the reagent tube 10. Rotating the reagent tube 10 to make the magnetic sleeve 50 positioned right above the lysis cavity 12; the automatic operation instrument drives the magnetic sleeve 50 to move downwards, and the magnetic sleeve 50 is used for puncturing the puncture part 32 corresponding to the cracking cavity 12, so that the magnetic sleeve 50 can extend into the cracking cavity 12. The above steps are repeated until the puncture parts 32 corresponding to the elution cavities 14 and the like are all punctured, so as to ensure that the magnetic sleeve 50 can extend into the lysis cavity 12, the elution cavity 14 and the like for operation.

Further, referring to fig. 2, the piercing part 32 is recessed toward the inside of the reagent vessel 10. Because the puncture part 32 is sunken towards the direction of the reagent cavity, the magnetic component can be prevented from deviating from the puncture part 32 in the process of puncturing, and the magnetic component can be ensured to puncture the puncture part 32 smoothly and quickly.

The depth of the depression of the puncture part 32 into the reagent chamber may be set according to actual requirements, and the depth of the depression of the puncture part 32 is specifically defined in this part. Of course, in other embodiments, the piercing portion 32 may also be planar.

In one embodiment, the second sealing cover 30 is at least one of a plastic cover and an aluminum film cover. Therefore, on one hand, the plastic cover and the aluminum film cover can not only seal the opening of the reagent tube 10, but also limit the lysate, the eluent and the like in the reagent tube 10; on the other hand, it is advantageous for the magnetic sleeve 50 to rapidly pierce the second sealing cap 30.

Optionally, the second sealing cap 30 is sealingly connected to the reagent vessel 10 by ultrasonic welding. Specifically, the ultrasonic wave is applied to the contact surface of the second sealing cover 30 and the reagent tube 10, so that the boundary between the reagent tube 10 and the second sealing cover 30 is melted, and the reagent tube 10 and the second sealing cover 30 are fused into a whole under a certain pressure. Thus, the method is environment-friendly and safe, and the fusion strength is high.

In one embodiment, referring to fig. 2 and 4, the reagent vessel 10 further comprises a wash chamber 13. Specifically, the washing chamber 13 contains washing liquid. After a sample to be detected is cracked in the cracking cavity 12 to obtain a target detection object, the magnetic beads adsorb the target detection object, the magnetic beads adsorbing the target detection object are transferred into the washing cavity 13 under the cooperation of the magnetic sleeve 50 and the magnetic rod, and the washing liquid is used for washing the magnetic beads to remove impurities. After washing, the beads are transferred to the elution chamber 14 for elution. Through set up washing chamber 13 in reagent pipe 10, can wash the adsorbed impurity of magnetic bead, improve the accuracy and the reliability of testing result. Also, the washing chamber 13 can collect and store discarded magnetic beads.

Further, referring to fig. 2 and 4, a sample chamber 11 and a magnetic bead chamber 15 are further disposed in the reagent tube 10. The sample cavity 11 is arranged in the middle of the reagent tube 10 and is communicated with the lysis cavity 12. The lysis chamber 12, the magnetic bead chamber 15, the washing chamber 13 and the elution chamber 14 are arranged around the sample chamber 11. Specifically, the sample chamber 11 is disposed corresponding to the first sample application hole 211, and the lysis chamber 12, the magnetic bead chamber 15, the washing chamber 13, and the elution chamber 14 are located on a circumference centered on the sample chamber 11. Therefore, in the process of sample detection, the reagent tube 10 is rotated, and the magnetic sleeve 50 is not required to be moved, so that the magnetic sleeve 50 can be positioned above the lysis cavity 12, the magnetic bead cavity 15, the washing cavity 13 and the elution cavity 14, detection is facilitated, and the detection efficiency is improved.

Specifically, in this embodiment, six reagent chambers are provided. One sample chamber 11 is provided, and one sample chamber 11 is provided in the middle of the reagent tube 10. The lysis chamber 12, the magnetic bead chamber 15 and the elution chamber 14 are all provided with one, and the washing chamber 13 is provided with two, and one lysis chamber 12, the magnetic bead chamber 15, the elution chamber 14 and two washing chambers 13 encircle the sample chamber 11.

In one embodiment, the bottom of the lysis chamber 12, the washing chamber 13, the elution chamber 14 and the magnetic bead chamber 15 is a hollow cylinder. So, at the in-process of sample detection, the magnetic bead is less with the bottom distance of magnetic sleeve 50, the magnetic sleeve 50 of being convenient for and magnetic rod absorption reagent intraductal magnetic bead.

Alternatively, the shapes of the lysis chamber 12, the washing chamber 13, the magnetic bead chamber 15 and the elution chamber 14 may be various shapes, such as triangular, square, polygonal, elliptical, circular or irregular.

In one embodiment, the reagent tube 10, the rotary cap 21, the reaction vessel 40 and the magnetic sleeve 50 are all integrally formed plastic bodies. Therefore, the processing is convenient, and the processing cost is low.

Optionally, the reagent vessel 10 is at least one of cylindrical, square cylindrical, polygonal cylindrical and irregular cylindrical. In particular, in this embodiment, the reagent vessel 10 is of cylindrical configuration.

In one embodiment, the first end of the magnetic sleeve 50 is sealed, and the second end of the magnetic sleeve 50 is provided with an opening, and the magnetic rod can be inserted into the magnetic sleeve 50 from the opening and can also be extracted from the magnetic sleeve 50. Thus, the magnetic rod is inserted into the magnetic sleeve 50 from the opening of the second end of the magnetic sleeve 50 and is matched with the magnetic sleeve 50 to adsorb magnetic beads, so that the transfer of a target detection object in a sample to be detected is realized; the magnetic wand is withdrawn from the magnetic sleeve 50 to release the magnetic beads.

Further, the first end of the magnetic sleeve 50 is provided with a piercing part 51. Optionally, the piercing portion 51 is of an inverted cone shape. Thus, the piercing part 51 is arranged, so that the cavity wall of the elution cavity 14 can be pierced quickly, and the eluent can flow into the reaction disc quickly for reaction detection.

In this embodiment, the following example of target detection substance extraction and PCR detection is further described, and specifically includes the following steps:

1. sample application

The first sealing cover 22 is opened, and the sample to be tested is added into the lysis chamber 12 from the first sample addition hole 211. The first sealing cap 22 is closed and the sample testing device is placed in the automated instrument.

2. Target detection substance extraction and PCR detection

1) Puncturing the second sealing cover: starting the automatic operation instrument, and moving the magnetic sleeve 50 upwards to the upper part of the reagent tube 10; rotating the reagent tube 10 so that the magnetic sleeve 50 is positioned over the elution chamber 14; then, the magnetic sleeve 50 is moved downward and the second sealing cap 30 at the corresponding position is pierced, so that the magnetic sleeve 50 can extend into the reagent chamber. The above steps are repeated until all the piercing portions 32 are pierced.

2) Sample lysis: moving the magnetic sleeve 50 upwards to the upper part of the reagent tube 10, and rotating the reagent tube 10 to enable the magnetic sleeve 50 to be positioned above the cracking cavity 12; then, the magnetic sleeve 50 is moved downwards and inserted into the lysis chamber 12, and the magnetic sleeve 50 rotates or moves up and down in the lysis chamber 12 to uniformly mix and lyse the sample to be tested in the lysis chamber 12.

3) Target detection object capture: moving the magnetic sleeve 50 upwards to the upper part of the reagent tube 10, and rotating the reagent tube 10 to enable the magnetic sleeve 50 to be positioned above the magnetic bead cavity 15; then, the magnetic sleeve 50 moves downwards and is inserted into the magnetic bead cavity 15, and the magnetic sleeve 50 rotates or moves up and down in the magnetic bead cavity 15 to uniformly mix the magnetic beads in the magnetic bead cavity 15; the magnetic rod moves downwards and is inserted into the magnetic sleeve 50 to adsorb the magnetic beads in the magnetic bead cavity 15; the magnetic sleeve 50 and the magnetic rod move upwards, so that the magnetic sleeve 50 and the magnetic rod are positioned above the cracking cavity 12; then, the magnetic sleeve 50 and the magnetic rod move downward into the lysis chamber 12, the magnetic rod moves upward to release the magnetic beads adsorbed on the magnetic sleeve 50, the magnetic sleeve 50 rotates or moves up and down in the lysis chamber 12 to uniformly mix the reagent and the magnetic beads in the lysis chamber 12, so that the target detection object in the sample to be detected is adsorbed on the magnetic beads.

4) First washing: the magnetic bar is inserted into the magnetic sleeve 50 downwards to adsorb the magnetic beads in the cracking cavity 12; the magnetic sleeve 50 and the magnetic rod move upwards together to the upper part of the cracking cavity 12; rotating the reagent tube 10 so that the magnetic sleeve 50 is positioned above the first washing chamber 13; then, the magnetic sleeve 50 and the magnetic rod move downward into the first washing chamber 13, the magnetic rod moves upward to release the magnetic beads adsorbed on the magnetic sleeve 50, and the magnetic sleeve 50 rotates or moves up and down in the first washing chamber 13 to mix the reagent and the magnetic beads in the first washing chamber 13.

5) And (3) second washing: the magnetic rod is inserted into the magnetic sleeve 50 downwards to adsorb the magnetic beads in the first washing cavity 13; the magnetic sleeve 50 and the magnetic bar move upwards to the upper part of the first washing cavity 13, and the reagent tube 10 is rotated to enable the magnetic sleeve 50 to be positioned above the second washing cavity; then, the magnetic sleeve 50 and the magnetic rod are moved downwards into the second washing chamber 13, the magnetic rod moves upwards to release the magnetic beads adsorbed on the magnetic sleeve 50, and the magnetic sleeve 50 rotates or moves upwards and downwards in the second washing chamber 13 to mix the reagent and the magnetic beads in the second washing chamber 13.

6) Elution of target detection: rotating the reagent tube 10 so that the magnetic sleeve 50 is positioned over the elution chamber 14; then, the magnetic sleeve 50 and the magnetic rod are moved downwards into the elution chamber 14, the magnetic rod moves upwards to release the magnetic beads adsorbed on the magnetic sleeve 50, and the magnetic sleeve 50 rotates or moves upwards and downwards in the elution chamber 14 to mix the reagent and the magnetic beads in the elution chamber 14.

7) Discarding magnetic beads: the magnetic bar is inserted into the magnetic sleeve 50 downwards to adsorb the magnetic beads in the elution cavity 14; the magnetic sleeve 50 and the magnetic bar move upwards to the upper part of the elution cavity 14, and the reagent tube 10 is rotated to enable the magnetic sleeve 50 to be positioned above the second washing liquid cavity; then, the magnetic sleeve 50 and the magnetic rod are moved downwards into the second washing chamber 13, the magnetic rod moves upwards to release the magnetic beads adsorbed on the magnetic sleeve 50, and the magnetic sleeve 50 rotates or moves upwards and downwards in the second washing chamber 13 to mix the reagent and the magnetic beads in the second washing chamber 13.

8) Detection of a target detection object: the magnetic sleeve 50 moves upwards to the upper part of the second washing cavity 13, and the reagent tube 10 is rotated to enable the magnetic sleeve 50 to be positioned above the elution cavity 14; then, the magnetic sleeve 50 is moved downwards into the elution chamber 14, the magnetic sleeve 50 is continuously moved downwards, the bottom of the elution chamber 14 is punctured, so that the eluent containing the target detection object flows into the reaction disc, the magnetic sleeve 50 downwards blocks and seals the pipe orifice of the reaction disc, the eluent containing the target detection object flows into the main flow channel 41, the reaction disc is rotated, so that the eluent in the main flow channel 41 enters the reaction chamber 44 and is mixed with the reagent or the freeze-dried powder in the reaction chamber 44; and starting a PCR program to detect the target detection object.

3. Sample detection device discard

And after the PCR is finished, taking the sample detection device out of the automatic operation instrument for waste biological treatment.

In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate the position or positional relationship based on the position or positional relationship shown in the drawings, and are only for convenience of description and simplified description, but not for convenience of description

The device or element so referred to must be oriented, constructed and operated in a particular manner and should not be construed as limiting the invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present application, unless expressly stated or limited otherwise, the first feature may be directly on or directly under the second feature or indirectly via intermediate members. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. A sample testing device, comprising:

2. The sample testing device of claim 1, wherein a sealing member is disposed in said through-hole, and said magnetic sleeve can pass through said sealing member and be sealingly engaged with said cap through said sealing member.

3. The apparatus according to claim 1, wherein the reaction container comprises a reaction tray, the reaction tray is provided with a main flow channel and at least two reaction chambers, the at least two reaction chambers are respectively communicated with the main flow channel, and the liquid in the elution chamber can respectively flow into the at least two reaction chambers through the main flow channel.

4. The apparatus according to claim 3, wherein the main channel is provided with at least two first channel segments, and the first channel segments extend in a direction away from the center of the reaction tray and are bent to extend in a direction close to the center of the reaction tray; the bending position of the first channel section is provided with a first branch flow channel, the first branch flow channel is located on one side, away from the center of the reaction disc, of the main flow channel, and the reaction chamber is communicated with the main flow channel through the first branch flow channel.

5. The sample detection device according to claim 3, wherein the main flow channel is an arc-shaped flow channel, the arc-shaped flow channel extends along a circumferential direction of the reaction disk, at least two first branch flow channels are arranged on a side of the arc-shaped flow channel away from a center of the reaction disk, the at least two first branch flow channels are arranged along an extending direction of the arc-shaped flow channel, and the reaction chamber is communicated with the arc-shaped flow channel through the first branch flow channels.

6. The sample detection device according to claim 3, wherein the reaction chamber comprises at least two chambers, and at least two chambers are disposed on one side of the main flow channel away from the center of the reaction disk and are spaced apart from each other in a radial direction of the reaction disk; the reaction disc is also provided with a second branch flow channel, and the two adjacent cavities are communicated through the second branch flow channel.

7. The apparatus according to any one of claims 1 to 6, wherein the tube cover comprises a first sealing cover and a rotating cover, the rotating cover is rotatably mounted on the open end of the reagent tube, the first sample application hole is formed in the rotating cover, and the first sealing cover is used for sealing the first sample application hole.

8. The apparatus according to any one of claims 1 to 6, further comprising a second sealing cap disposed at the opening of the reagent tube, wherein the second sealing cap has a second sample application hole disposed corresponding to the first sample application hole.

9. The apparatus according to claim 8, wherein the second sealing cap has a hollow guiding column extending from the edge of the second loading hole to a direction close to the tube cap, and the guiding column is inserted into the first loading hole.

10. The apparatus according to claim 8, wherein the second sealing cap has at least two piercing portions, and the at least two piercing portions are disposed in one-to-one correspondence with the lysis chamber and the elution chamber; the puncture part is arranged towards the inner recess of the reagent tube.

11. The sample testing device of claim 8, wherein said second sealing cover is at least one of a plastic cover and an aluminum film cover.

12. The sample detection device according to any one of claims 1 to 6, wherein a sample chamber, a magnetic bead chamber and a washing chamber are further disposed in the reagent tube, and the sample chamber is disposed in the middle of the reagent tube and is communicated with the lysis chamber; the lysis cavity, the magnetic bead cavity, the washing cavity and the elution cavity are arranged around the sample cavity.

13. The sample detection device according to any one of claims 1 to 6, wherein a first end of the magnetic sleeve is sealed, a second end of the magnetic sleeve is provided with an opening, and the magnetic rod can be inserted into the magnetic sleeve from the opening and can also be extracted from the magnetic sleeve; the first end of the magnetic sleeve is provided with a puncture part.

14. The apparatus according to any one of claims 1 to 6, wherein the bottoms of the lysis chamber and the elution chamber are hollow cylinders.