CN117504797A - Synthetic column structure and biochemical reaction equipment - Google Patents
Synthetic column structure and biochemical reaction equipment Download PDFInfo
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- CN117504797A CN117504797A CN202311849574.1A CN202311849574A CN117504797A CN 117504797 A CN117504797 A CN 117504797A CN 202311849574 A CN202311849574 A CN 202311849574A CN 117504797 A CN117504797 A CN 117504797A
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- 238000005842 biochemical reaction Methods 0.000 title claims abstract description 68
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 73
- 238000006243 chemical reaction Methods 0.000 claims abstract description 72
- 238000003786 synthesis reaction Methods 0.000 claims description 27
- 230000015572 biosynthetic process Effects 0.000 claims description 25
- 239000002131 composite material Substances 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 7
- 238000004891 communication Methods 0.000 claims description 6
- 238000012545 processing Methods 0.000 abstract description 3
- 238000007493 shaping process Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 22
- 230000007246 mechanism Effects 0.000 description 11
- 239000002699 waste material Substances 0.000 description 11
- 238000001179 sorption measurement Methods 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 7
- 230000009286 beneficial effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000004696 Poly ether ether ketone Substances 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 238000007599 discharging Methods 0.000 description 4
- 229920002530 polyetherether ketone Polymers 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- -1 polypropylene Polymers 0.000 description 3
- 239000004743 Polypropylene Substances 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 229920001155 polypropylene Polymers 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920006169 Perfluoroelastomer Polymers 0.000 description 1
- 229920001774 Perfluoroether Polymers 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000005388 borosilicate glass Substances 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000003475 lamination Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- UJMWVICAENGCRF-UHFFFAOYSA-N oxygen difluoride Chemical compound FOF UJMWVICAENGCRF-UHFFFAOYSA-N 0.000 description 1
- 229920002493 poly(chlorotrifluoroethylene) Polymers 0.000 description 1
- 239000005023 polychlorotrifluoroethylene (PCTFE) polymer Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/24—Stationary reactors without moving elements inside
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B21/00—Arrangements or duct systems, e.g. in combination with pallet boxes, for supplying and controlling air or gases for drying solid materials or objects
Abstract
The utility model provides a synthetic post structure and biochemical reaction equipment, synthetic post structure includes the sleeve pipe on base and cover locate the base, and a reaction chamber is enclosed to base and sleeve pipe, and the reaction chamber is used for holding biochemical reaction chip and reagent, and the base includes the runner with the reaction chamber intercommunication, runner and reagent source intercommunication, the base still including being located the intraductal tip of cover, and the tip includes the terminal surface towards the reaction chamber, be equipped with a plurality of first recesses on the terminal surface. Be equipped with a plurality of first recesses on the terminal surface that the base is located the reaction chamber in this application among the synthetic post structure, make biochemical reaction chip easily pour, and can reduce reagent and remain, promote biochemical reaction quality, this synthetic post structure's simple structure, the processing degree of difficulty of each part is low, easily shaping, low cost.
Description
Technical Field
The invention relates to the technical field of synthesis and analysis of biochemical substances, in particular to a synthesis column structure and biochemical reaction equipment.
Background
The synthesis column is a key component in the gene synthesis equipment, and in the gene synthesis process, a synthesis chip is required to be placed in the synthesis column, and required reagents are added into the synthesis column to perform synthesis reaction. However, the existing synthetic columns have the following drawbacks: after one round of synthesis is completed, the synthesis chip is easy to remain in the synthesis column and is not easy to pour out; after the reagent is discharged, reagent residues are easy to appear in the synthesis column, and the gene synthesis quality is affected.
Disclosure of Invention
In order to solve at least one of the above drawbacks, it is necessary to provide a synthesis column structure and a biochemical reaction apparatus.
The embodiment of the application provides a synthetic column structure, the synthetic column structure includes: the base is arranged on the base in a sleeved mode, a reaction cavity is formed by surrounding the base and the sleeve, the reaction cavity is used for accommodating a biochemical reaction chip and reagents required by biochemical reaction, the base comprises a runner communicated with the reaction cavity, the runner is used for being communicated with a reagent source for storing the reagents, the base further comprises an end portion located in the sleeve, the end portion comprises an end face facing the reaction cavity, and a plurality of first grooves are formed in the end face.
In some possible embodiments, the plurality of first grooves are disposed at radial intervals with respect to a central axis of the base.
In some possible embodiments, the plurality of first grooves includes at least one first groove and a plurality of second grooves, the first grooves are disposed through the central shaft along a diameter direction of the end face, the plurality of second grooves are different in size along the diameter direction of the end face, and the plurality of second grooves having different sizes are alternately arranged along a circumferential direction of the end face.
In some possible embodiments, the opening of the first groove has a circumferential dimension along the end face that is less than 1/2 of the smallest dimension of the biochemical reaction chip.
In some possible embodiments, the end surface includes a top portion near a central axis of the base and an edge portion near the sleeve, a gap is provided between the edge portion and the sleeve, a dimension of the end surface in a direction perpendicular to the central axis increases gradually from the top portion to the edge portion, the first groove extends from the top portion to the edge portion, and the first groove communicates with the gap.
In some possible embodiments, the end face is an arcuate face protruding toward one side of the reaction chamber.
In some possible embodiments, the flow channel comprises a main flow channel for communicating with the reagent source and a split flow channel, the split flow channel being located at the end and communicating with the main flow channel and the reaction chamber, respectively, the main flow channel extending in a direction of a central axis of the base and the split flow channel extending in a direction perpendicular to the central axis.
In some possible embodiments, the side of the end portion, which is close to the sleeve, is recessed toward the central axis of the base to form a second groove, the second groove is communicated with the reaction chamber, and the opening of the sub-channel is located in the second groove.
In some possible embodiments, the base further comprises a connecting part connected with the end part, the sleeve is sleeved outside the connecting part, and a sealing ring is arranged between the connecting part and the sleeve; the synthesis column structure further comprises an upper cover detachably connected with the sleeve, and an air passage communicated with the reaction cavity is further formed in the upper cover.
Embodiments of the present application also provide a biochemical reaction apparatus comprising at least one synthetic column structure as described above.
The base is equipped with a plurality of first recesses on the terminal surface that is located the reaction chamber in the above-mentioned synthetic post structure that this embodiment provided, can effectively reduce the area of contact of biochemical reaction chip and terminal surface, and then reduce the risk of formation plane absorption, make the biochemical reaction chip be difficult for being adsorbed, thereby easily pour out, the terminal surface that is provided with a plurality of first recesses has increased air-dried air current passageway, the drying at the position that the terminal surface was pressed close to the biochemical reaction chip is more favorable to, the effect of water conservancy diversion can also be played to first recess simultaneously, can promote the discharge of chip below reagent, reduce the biochemical reaction chip adhesion that the liquid remains to cause, the biochemical reaction chip can be thoroughly dried and pour out smoothly after guaranteeing that the biochemical reaction is accomplished in the reaction chamber, there is not the chip to remain in the reaction chamber. The end face inclined from the top to the edge is favorable for forming an inclined reagent circulation channel, reagent residues in the reaction cavity, especially reagent residues below the biochemical reaction chip are reduced, so that the reagent discharge action at each time is more thorough, the interference of the reagent residues on the biochemical reaction effect is reduced, and the quality of the biochemical reaction is improved. In addition, the synthetic column structure has the advantages of simple structure, low processing difficulty of each part, easy molding and low cost.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments of the present invention will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic structural diagram of a synthetic column structure according to an embodiment of the present disclosure.
Fig. 2 is an enlarged view of the portion I in fig. 1.
Fig. 3 is a schematic perspective view of the base in fig. 1.
Fig. 4 is a schematic view of an end face in another embodiment of the present application.
Fig. 5 is a schematic view of the structure of an end face in a further embodiment of the present application.
Fig. 6 is a schematic view of the structure of an end face in a further embodiment of the present application.
Fig. 7 is a front view of the base of fig. 3.
Fig. 8 is a cross-sectional view of fig. 7.
FIG. 9 is a block diagram of a biochemical reaction apparatus according to an embodiment of the present application.
Description of the main reference signs
A composite column structure 100; a base 1; a sleeve 2; a reaction chamber 3; a first end 4; an end 41; an end face 42; a top surface 421; a bevel 422; a first groove 44; a first groove 441; a second slot 442; a second groove 45; a connection portion 43; a first portion 431; a second portion 432; a third groove 433; a second end 5; a joint screw hole 51; a gap 6; a flow channel 7; a main flow passage 71; a subchannel 72; a seal ring 8; an upper cover 9; an air path 91; a biochemical reaction apparatus 200; an air pressure control device 201; an upper cover driving mechanism 202; a base drive mechanism 203; a reagent storage device 204; a waste liquid collection device 205; a control device 206; a top A; an edge portion B; a central axis a.
The invention will be further described in the following detailed description in conjunction with the above-described figures.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It is noted that when an element is referred to as being "fixed" to "or" mounted on "another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "disposed on" another element, it can be directly on the other element or intervening elements may also be present. The term "and/or" as used herein includes all and any combination of one or more of the associated listed items.
Referring to fig. 1 and 2, a composite column structure 100 is provided in an embodiment of the present application, and the composite column structure 100 includes: the base 1 and the sleeve 2 sleeved on the base 1 enclose a reaction cavity 3, the reaction cavity 3 is used for containing biochemical reaction chips and reagents required by biochemical reaction, the base 1 comprises a runner 7 communicated with the reaction cavity 3, the runner 7 is used for being communicated with a reagent source for storing the reagents, the base 1 further comprises an end 41 positioned in the sleeve 2, the end 41 comprises an end face 42 facing the reaction cavity 3, and a plurality of first grooves 44 are formed in the end face 42.
Referring to fig. 3 in combination, the base 1 includes a first end 4 and a second end 5 disposed opposite to each other, the sleeve 2 is sleeved on the outer edge of the first end 4, and encloses a reaction chamber 3 with the first end 4, and the first end 4 includes an end 41, i.e., an end face 42 is located on the first end 4. The end face 42 comprises a top portion a near the central axis a of the base 1, said top portion a being distant from the second end 5 and an edge portion B near the second end 5, and near the sleeve 2. In some embodiments, the end surface 42 may be a curved surface structure, that is, extending from the top portion a to the edge portion B to form the curved surface structure, where the top portion a may be a point or an area having a certain area around the central axis a. That is, the end surface 42 is disposed obliquely from the central axis a of the base 1 to the outer edge, thereby forming an end surface 42 obliquely downward from the top portion a to the edge portion B, which defines herein that the second end 5 is located below with respect to the first end 4.
In some embodiments, from the top portion a to the edge portion B, the dimension of the end face 42 along the direction perpendicular to the central axis a is gradually increased, so that the end face 42 forms an inclined curved surface structure, and thus the biochemical reaction chip cannot form planar adsorption between the reaction chamber 3 and the end face 42, which can effectively reduce the risk that the biochemical reaction chip is adsorbed on the end portion 41 and is difficult to be taken out, and the inclined end face 42 is further beneficial to forming an inclined reagent circulation channel, reducing reagent residues in the reaction chamber 3, especially reagent residues below the biochemical reaction chip, so that each reagent discharge action is ensured to be more thorough, less reagent residues interfere with biochemical reaction effects, and further improving the quality of biochemical reactions.
In some embodiments, the curved surface structure of the end surface 42 may be an arc surface protruding toward the reaction cavity 3, so that the end portion 41 forms an arc surface structure protruding toward the reaction cavity 3, so that the biochemical reaction chip does not form planar adsorption between the inside of the reaction cavity 3 and the end portion 41, and the risk that the biochemical reaction chip is adsorbed on the end portion 41 and is difficult to be taken out can be effectively reduced; in addition, the arc-shaped surface can guide the flow of the reagent, so that the reagent residue below the chip is avoided, and the quality of biochemical synthesis reaction is improved. It can be appreciated that the radian of the arc surface can be designed according to actual needs, and the radian of the part of the end surface 42, which is close to the central axis a, can be designed to be smaller, so that a supporting platform can be provided for the chip, and the radian of the part of the end surface 42, which is close to the sleeve 2, can be designed to be larger, so that plane adsorption between the chip and the end surface 42 can be avoided, reagent flow can be better guided, and reagent residues below the chip can be avoided.
It will be appreciated that in another embodiment, as shown in fig. 4, the curved surface structure of the end surface 42 may include an arc surface recessed toward a side away from the reaction chamber 3, that is, a concave surface structure is formed on the end portion 41, which may also achieve a reduction of the risk of planar adsorption between the chip and the end portion 41, and may also play a role of guiding flow to prevent reagent residues.
It will be further appreciated that in yet another embodiment, as shown in fig. 5, the curved structure of the end surface 42 may further include two parts, that is, a top surface 421 near the central axis a and an inclined surface 422 connected to the periphery of the top surface 421, where the top portion a is located on the top surface 421, and the edge portion B is the edge of the inclined surface 422. Specifically, the top surface 421 may be an arc surface as described above, or may be a plane, and the inclined surface 422 may be an inclined plane, or may be an inclined arc surface. Thus, the chip can be supported, planar adsorption between the chip and the end 41 can be avoided, and in addition, the outflow of the reagent below the chip is facilitated, and reagent residue is avoided.
It will be further appreciated that in yet another embodiment, as shown in fig. 6, the curved surface structure of the end surface 42 may also be a wave-shaped structure having a plurality of wave-shaped protrusions and depressions, where the wave-shaped structure may reduce planar adsorption between the biochemical reaction chip and the end surface 42, which is beneficial for pouring out the chip, and the depression of the wave-shaped structure may also act as a flow guiding groove, which is beneficial for reagent discharge.
Referring to fig. 1 to 3 again, by arranging a plurality of first grooves 44 on the end face 42, the contact area between the biochemical reaction chip and the end face 42 can be effectively reduced, and the risk of planar adsorption is reduced, so that the biochemical reaction chip is not easy to adsorb, and is easy to pour out, the end face 42 provided with the plurality of first grooves 44 increases an air-dried air flow channel, drying of the part of the biochemical reaction chip close to the end face 42 is more facilitated, meanwhile, the first grooves 44 can play a role of guiding flow, the discharge of reagents below the chip can be promoted, adhesion of the biochemical reaction chip caused by liquid residues is reduced, the biochemical reaction chip can be thoroughly dried and smoothly poured out after the biochemical reaction in the reaction cavity is ensured, and no chip remains in the reaction cavity 3.
In some embodiments, when the end face 42 is a curved surface structure inclined from the top to the edge, the side wall of the end 41 is not in close contact with the sleeve 2, the edge B and the sleeve 2 have a gap 6 therebetween, the first groove 44 extends from the top a to the edge B, and the first groove 44 communicates with the gap 6, i.e., the open end of the first groove 44 penetrates the side wall of the end 41 to communicate with the gap 6, so that reagents can flow into or out of the reaction chamber 3. The first groove 44 is designed to guide the reagent, so that the reagent under the chip can smoothly flow into the gap 6 along the first groove 44 and further flow out of the reaction chamber 3. In addition, the end face 42 has a plurality of first grooves 44, which can further reduce the risk of planar adsorption between the chip and the end face 42. The specific structure of the first grooves 44 may be designed according to actual needs, and the plurality of first grooves 44 may be uniformly distributed on the end face 42, or may be unevenly distributed on the end face 42, or may extend from the top a of the end face 42 to the edge portion B, or may extend from a position near the top a to the edge portion B. It will be appreciated that since the size of the biochemical reaction chip is generally small, the size of the opening where the gap 6 communicates with the reaction chamber 3 needs to be designed to be small in order to prevent the chip from being accidentally discharged from the gap 6, i.e., only the reagent is discharged to leave the chip. In some embodiments, the opening size of the gap 6 is smaller than the smallest dimension of the chip, for example, when the chip size is 2mm by 0.45mm, i.e., the smallest dimension (thickness) is 0.45mm, the opening size of the gap 6 may be designed to be smaller than 0.45mm, for example, may be 0.15mm.
In some embodiments, a plurality of the first grooves 44 are disposed at radial intervals with respect to the central axis a. Further, the plurality of first grooves 44 includes at least one first groove 441 and a plurality of second grooves 442, the first grooves 441 are disposed along the diameter direction of the end face 42 and penetrate through the central axis a, the plurality of second grooves 442 are different in size along the diameter direction of the end face 42, and the plurality of second grooves 442 with different sizes are alternately arranged along the circumferential direction of the end face 42 to form an umbrella-shaped arrangement, so that fine micro grooves are prevented from being generated in the center, and uniform channels radiating outwards from the top a are formed on the end face 42, thereby facilitating the diversion of gas and liquid.
In some embodiments, the opening size (which may be defined as the width) of the first groove 44 is smaller than 1/2 of the minimum size of the biochemical reaction chip along the circumferential direction of the end face 42, and typically the minimum size of the biochemical reaction chip may be the thickness of the chip, for example, when the thickness of the biochemical reaction chip is 0.45mm, the design width of the first groove 44 may be smaller than or equal to 0.2mm, and the chip may be prevented from being jammed into the first groove 44. Specifically, the width of the first groove 44 may be 0.1mm to 0.5mm, and may specifically be a micro groove of 0.2 mm. The curved end face 42 combines the design of the first groove 44, so that the problems of plane adsorption, reagent residue and the like caused by the lamination of the chip and the base 1 can be effectively avoided, the reagent can be thoroughly discharged out of the reaction cavity 3 each time, and the chip can be thoroughly dried and smoothly poured out of the reaction cavity 3.
Referring to fig. 7 and 8, referring to fig. 1 and 2 in combination, the base 1 further includes a flow channel 7, the flow channel 7 includes a main flow channel 71 and a split flow channel 72, wherein the main flow channel 71 penetrates through the second end 5 and extends into a portion of the first end 4, the split flow channel 72 is located at the first end 4, and the split flow channel 72 is respectively communicated with the main flow channel 71 and the reaction chamber 3, so as to realize communication between the reaction chamber 3 and the main flow channel 71, and realize liquid feeding and discharging of the reaction chamber 3.
In some embodiments, the primary flow channel 71 extends in a direction along the central axis a, and the secondary flow channel 72 extends in a direction perpendicular to the central axis a. The number of the flow dividing channels 72 can be one or a plurality, for example, 2, 4 or 6 flow dividing channels 72 can be designed according to the actual situation, and the flow dividing channels 72 can realize rapid and uniform liquid feeding and discharging. In some embodiments, the plurality of shunt channels 72 may be evenly distributed.
In some embodiments, the openings of the sub-channels 72 are located on the side wall of the end 41 near the sleeve 2, so that communication between the sub-channels 72 and the reaction chamber 3 can be achieved through the gap 6, and reagent feeding and draining can be achieved. In some embodiments, the width of the gap 6 is around 0.15mm, which enables the end 41 to have a sufficiently large bearing area, and also enables the feeding and discharging of reagents, while ensuring that the chip is held.
In some examples, the end 41 is recessed towards the central axis a near the side wall of the sleeve 2 to form a second groove 45, the second groove 45 being in communication with the reaction chamber 3, in particular the second groove 45 being in communication with the reaction chamber 3 through the gap 6 between the end 41 and the sleeve 2. The opening of the flow dividing channel 72 is located on the second groove 45, and the design of the second groove 45 can reduce the pressure of the reagent in the flow dividing channel 72 to the sleeve 2, so that the buffer effect is achieved, the reagent flow is more stable, and the reagent flow efficiency can be improved.
In some embodiments, the primary flow channel 71 within the second end 5 may be in communication with a reagent source that stores reagents and a waste pool that collects waste. The second end 5 is provided with a joint threaded hole 51, and a liquid path is connected to the joint threaded hole 51 and can be connected with equipment such as a reagent source or a waste liquid tank so as to realize liquid injection and liquid discharge functions.
Referring to fig. 3 and 8, the first end 4 further includes a connecting portion 43 connected to the end 41, and the sleeve 2 is sleeved outside the connecting portion 43. In some embodiments, the connecting portion 43 includes a first portion 431 connected to the end 41 and a second portion 432 connected to the first portion 431 and the second end 5, where the sleeve 2 is sleeved on the outer side of the first portion 431, the first portion 431 is located inside the sleeve 2, the second portion 432 is located on the outer side of the sleeve 2, and the second portion 432 protrudes away from the central axis a, so that the second portion 432 forms an L-shaped step with the first portion 431, and the end of the sleeve 2 abuts against a surface of the second portion 432 close to the first portion 431, so that the sleeve 2 is more stably sleeved on the base 1.
Be equipped with sealing washer 8 between base 1 and the sleeve pipe 2, sealing washer 8 can make between sleeve pipe 2 and the base 1 be in sealing state, prevents that the reagent from revealing to realize the sealed detachable connection of base 1 and sleeve pipe 2. Specifically, the surface of the first portion 431, which is close to the sleeve 2, is recessed toward the central axis a to form a third groove 433, and the sealing ring 8 is located in the third groove 433, so that the sealing ring 8 can be stabilized, and the connection stability of the base 1 and the sleeve 2 is improved. In some embodiments, the seal ring 8 may be an O-ring, and the specific material may be, but is not limited to, perfluoroether rubber (FFKM).
The synthesis column structure 100 further comprises an upper cover 9 detachably connected with the sleeve 2, the upper cover 9 is further provided with an air passage 91 communicated with the reaction cavity 3, the air passage 91 is communicated with the air pressure control device to realize pressurization or pressure relief of the reaction cavity 3, for example, the reaction cavity 3 is depressurized through the air pressure control device and the air passage 91 on the upper cover 9 during liquid injection, and the reaction cavity 3 is inflated and pressurized through the air pressure control device and the air passage 91 on the upper cover 9 during liquid discharge. In addition, the upper cover 9 is also connected to an upper cover driving mechanism for effecting movement of the upper cover 9.
In some embodiments, the material of the sleeve 2 may be transparent glass, such as quartz glass or borosilicate glass, and other materials compatible with biochemical reagents, such as transparent polypropylene (PP), may be used. The diameter and length of the sleeve 2 can be designed reasonably according to the flux.
In some embodiments, the material of the base 1 is not limited to Polyetheretherketone (PEEK), polytetrafluoroethylene (PTFE), polytrifluoroethylene (PCTFE), sus316L, and the like. Specifically, the base 1 may be made of PEEK material, and the base 1 made of PEEK material not only ensures reagent compatibility, but also has good workability, is easy to design the end face 42 into an arc surface, and is easy to form the first groove 44 on the end face 42.
When in use, the pressure in the reaction cavity 3 is released, the upper cover 9 is opened, a biochemical reaction chip (such as a gene synthesis chip) is placed in the reaction cavity 3, and a required reagent (such as a reagent required for gene synthesis) is injected into the reaction cavity 3 to perform biochemical reaction (such as a gene synthesis reaction); during liquid discharge, the reaction cavity 3 is inflated and pressurized, and the waste liquid after reaction in the reaction cavity 3 is discharged. After one round of synthesis task is completed through the edited liquid injection time sequence, the air channel on the air pressure control device and the upper cover 9 is used for inflating the reaction cavity 3, the chip is dried, after the chip is dried, the upper cover 9 is lifted under the pulling of the upper cover driving mechanism and separated from the sleeve 2, the synthesis column structure 100 is driven by the base driving mechanism to rotate, and the chip in the reaction cavity 3 is poured out onto the receiving tray.
The above-mentioned synthetic post structure 100 that this embodiment provided is equipped with a plurality of first recesses 44 on base 1 is located the terminal surface 42 in reaction chamber 3, can effectively reduce the area of contact of biochemical reaction chip and terminal surface 42, and then reduce the risk of formation plane adsorption, make the biochemical reaction chip be difficult for being adsorbed, thereby easily pour, the terminal surface 42 that is provided with a plurality of first recesses has increased air-dried air current passageway, more be favorable to the drying at the position that the biochemical reaction chip pressed close to the terminal surface, simultaneously the effect of water conservancy diversion can also be played to first recess 44, can promote the discharge of chip below reagent, reduce the biochemical reaction chip adhesion that the residual liquid caused, guarantee that biochemical reaction chip can thoroughly dry and pour smoothly after the biochemical reaction in reaction chamber 3 is accomplished, there is not the chip to remain in reaction chamber 3. The formed curved surface structure from the top A to the edge B is also beneficial to reducing the residual of the reagent in the reaction cavity 3, is more beneficial to the outflow of the reagent, and especially is beneficial to the residual of the reagent below the biochemical reaction chip, thereby ensuring that each reagent discharging action is more thorough, reducing the interference of the residual reagent on the biochemical reaction effect, and further improving the quality of the biochemical reaction. In addition, the composite column structure 100 has the advantages of simple structure, low processing difficulty of each part, easy molding and low cost.
The embodiment of the application also provides biochemical reaction equipment, which comprises the synthetic column structure. Specifically, the biochemical reaction equipment comprises at least one synthetic column structure, and can complete multiple rounds of biochemical reactions.
Specifically, as shown in fig. 9, which is a system frame diagram of the biochemical reaction apparatus 200, the biochemical reaction apparatus 200 includes the synthetic column structure 100 as described above, and further includes at least an air pressure control device 201, a cover driving mechanism 202, a base driving mechanism 203, a reagent storage device 204 (or a reagent source) storing various required reagents, a waste liquid collection device 205, and the like. The air pressure control device 201 is communicated with the reaction cavity 3 through an air passage on the upper cover 9 and is used for pressurizing or depressurizing the reaction cavity 3; the upper cover driving mechanism 202 is connected with the upper cover 9 and is used for driving the upper cover 9 to move so as to realize that the upper cover 9 covers the sleeve 2 or moves away from the sleeve 2; the base driving mechanism 203 is connected with the base 1 and is used for driving the whole synthetic column structure 100 to rotate and pouring out the biochemical reaction chip in the reaction cavity 3 from the opening of the upper cover 9; the reagent storage device 204 and the waste liquid collection device 205 are communicated with the reaction chamber 3 through the main flow channel 71, and the reagent storage device 204 and the waste liquid collection device 205 can be connected to the threaded joint hole of the second end 5, wherein the reagent storage device 204 is used for storing reagents required by biochemical reactions (such as reagents required by gene synthesis reactions) and providing the reagents for the reaction chamber 3, and the waste liquid collection device 205 is used for collecting waste liquid discharged from the reaction chamber 3 after the biochemical reactions. It will be appreciated that the biochemical reaction apparatus 200 may further comprise a control device 206, where the control device 206 is configured to control the co-operation of the synthesis column structure 100, the air pressure control device 201, the upper cover driving mechanism 202, the base driving mechanism 203, the reagent storage device 204, the waste liquid collecting device 205, and the like, so as to complete the biochemical reaction.
Finally, it should be noted that the above-mentioned embodiments are merely for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made to the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention.
Claims (10)
1. A composite column structure comprising: the base and the sleeve are sleeved on the base, the base and the sleeve enclose a reaction cavity, the reaction cavity is used for containing a biochemical reaction chip and reagents required by biochemical reaction, the base comprises a runner communicated with the reaction cavity, the runner is used for being communicated with a reagent source for storing the reagents,
the base also comprises an end part positioned in the sleeve, wherein the end part comprises an end face facing the reaction cavity, and a plurality of first grooves are formed in the end face.
2. The composite column structure according to claim 1, wherein a plurality of said first recesses are radially spaced relative to a central axis of said base.
3. The composite column structure according to claim 2, wherein the plurality of first grooves include at least one first groove provided through the central shaft in a diameter direction of the end face, and a plurality of second grooves having different sizes in a diameter direction of the end face, the plurality of second grooves having different sizes being alternately arranged in a circumferential direction of the end face.
4. The composite column structure according to claim 1, wherein the opening of the first groove has a circumferential dimension along the end face that is less than 1/2 of a minimum dimension of the biochemical reaction chip.
5. The composite column structure according to claim 1, wherein the end face includes a top portion near a central axis of the base and an edge portion near the sleeve with a gap therebetween, the end face gradually increasing in size in a direction perpendicular to the central axis from the top portion to the edge portion, the first groove extending from the top portion to the edge portion, and the first groove communicating with the gap.
6. The composite column structure according to claim 5, wherein said end face is an arcuate face protruding toward one side of said reaction chamber.
7. The synthesis column structure according to claim 1, wherein the flow channel comprises a main flow channel for communicating with the reagent source and a split flow channel, the split flow channel being located at the end and communicating with the main flow channel and the reaction chamber, respectively, the main flow channel extending in a direction of a central axis of the base and the split flow channel extending in a direction perpendicular to the central axis.
8. The composite column structure according to claim 7, wherein the side of the end portion adjacent to the sleeve is recessed toward the central axis of the base to form a second recess, the second recess is in communication with the reaction chamber, and the opening of the flow dividing channel is located in the second recess.
9. The composite column structure according to claim 1, wherein the base further comprises a connecting portion connected with the end portion, the sleeve is sleeved outside the connecting portion, and a sealing ring is arranged between the connecting portion and the sleeve;
the synthesis column structure further comprises an upper cover detachably connected with the sleeve, and an air passage communicated with the reaction cavity is further formed in the upper cover.
10. A biochemical reaction apparatus comprising at least one synthetic column structure according to any one of claims 1 to 9.
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| CN202311849574.1A CN117504797A (en) | 2023-12-29 | 2023-12-29 | Synthetic column structure and biochemical reaction equipment |
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| CN202311849574.1A CN117504797A (en) | 2023-12-29 | 2023-12-29 | Synthetic column structure and biochemical reaction equipment |
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