CN116966940A - Microfluidic chip and detection device - Google Patents

Microfluidic chip and detection device Download PDF

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Publication number
CN116966940A
CN116966940A CN202211209477.1A CN202211209477A CN116966940A CN 116966940 A CN116966940 A CN 116966940A CN 202211209477 A CN202211209477 A CN 202211209477A CN 116966940 A CN116966940 A CN 116966940A
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CN
China
Prior art keywords
flow channel
sealing
layer
microfluidic chip
cover body
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202211209477.1A
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Chinese (zh)
Inventor
王琛瑜
王强
张玙璠
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beijing Boe Zhi Microbial Technology Co ltd
Original Assignee
Beijing Boe Zhi Microbial Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Beijing Boe Zhi Microbial Technology Co ltd filed Critical Beijing Boe Zhi Microbial Technology Co ltd
Priority to CN202211209477.1A priority Critical patent/CN116966940A/en
Publication of CN116966940A publication Critical patent/CN116966940A/en
Pending legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0053—Details of the reactor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0053—Details of the reactor
    • B01J19/0073—Sealings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices

Abstract

The invention relates to a microfluidic chip, which comprises a detection box main body and a cover body, wherein a sample cavity for containing an extraction reagent and a reaction cavity positioned at one side of the sample cavity are arranged in the detection box main body; a flow passage opening is formed in one side, far away from the cover body, of the sample cavity, a blocking piece is arranged in the flow passage opening for sealing, and the blocking piece is configured to open the flow passage opening under preset conditions; the bottom of detecting the box main part is provided with first runner, detects the box main part and includes intermediate structure layer and is located intermediate structure layer and is kept away from the first sealing layer of one side of lid, and sample chamber and reaction chamber are located intermediate structure layer, and intermediate structure layer's bottom is protruding to the direction of keeping away from the lid to be equipped with first sealing edge, is provided with on first sealing edge or the first sealing layer and presets the recess for intermediate structure layer and first sealing layer surround, form first runner between first sealing edge and first sealing layer. The invention also relates to a detection device.

Description

Microfluidic chip and detection device
Technical Field
The invention relates to the technical field of detection, in particular to a microfluidic chip and a detection device.
Background
The microfluidic chip can realize the processes of sample loading, reaction, detection and the like by combining with technologies such as biology, chemistry, medicines and the like. The device is characterized by its effective structure (channels, reaction chambers and other certain functional components) for containing fluids, which is at least on a micrometer scale in one dimension. Due to the micro-scale structure, the fluid exhibits and produces therein specific properties that differ from those of the macro-scale. Thus developing unique analytical properties.
Disclosure of Invention
In order to solve the technical problems, the invention provides a microfluidic chip and a detection device, which solve the problem that aerosol pollution is easy to occur in detection by adopting the microfluidic chip.
In order to achieve the above purpose, the technical scheme adopted by the embodiment of the invention is as follows: the microfluidic chip comprises a detection box main body and a cover body, wherein a sample cavity for containing an extraction reagent and a reaction cavity positioned at one side of the sample cavity are arranged in the detection box main body;
a flow channel opening is formed in one side, far away from the cover body, of the sample cavity, a plugging piece is arranged in the flow channel opening for sealing, and the plugging piece is configured to open the flow channel opening under preset conditions;
the bottom of detecting the box main part is provided with first runner, first runner is used for the intercommunication runner mouth with the reaction chamber, detect the box main part including intermediate structure layer and be located intermediate structure layer is kept away from the first sealing layer of one side of lid, the sample chamber with the reaction chamber is located intermediate structure layer, intermediate structure layer's bottom is to keeping away from the direction of lid is equipped with first sealing edge, first sealing edge or be provided with preset groove on the first sealing layer, make intermediate structure layer with first sealing layer surrounds first sealing layer first sealing edge with form between the first sealing layer first runner.
Optionally, the method further comprises:
the air cavity is arranged on the middle structural layer;
and the second flow passage is used for communicating the air cavity and the reaction cavity.
Optionally, the sealing device further comprises a second sealing layer located on one side, close to the cover body, of the middle structure layer, and the cover body, the second sealing layer and the middle structure layer are surrounded to form the second flow channel.
Optionally, the sealing structure further comprises a second sealing layer located on one side, close to the cover body, of the intermediate structure layer, a second sealing edge is arranged on one side, close to the cover body, of the intermediate structure layer, and the intermediate structure layer and the second sealing layer are surrounded, so that a second flow channel is formed between the second sealing edge and the second sealing layer.
Optionally, a plurality of reaction chambers are arranged on the intermediate structure layer;
the second flow channel comprises a plurality of sub flow channels which are respectively communicated with the reaction cavities in a one-to-one correspondence manner;
the middle structure layer comprises a plurality of air cavities, and the air cavities are communicated with the sub-runners in a one-to-one correspondence mode.
Optionally, the first flow channel includes with the sprue of runner mouth intercommunication, and by the sprue to the extension of reaction chamber and intercommunication the branch flow channel of reaction chamber is provided with a plurality of intervals setting's wetting column in the sprue, adjacent two the interval between the wetting column is less than the sprue with the width of branch flow channel intercommunication department makes the shutoff piece is to keeping away from the direction flow of branch flow channel.
Optionally, the diameter of the wetting column is 1-3mm, and the distance between two adjacent wetting columns is 1-3mm.
Optionally, the maximum width of the end of the main runner far away from the branch runner is 8-15mm, and the width of the branch runner is 0.5-2mm.
Optionally, the cover body is covered on one side of the second sealing layer away from the middle structure layer.
Optionally, the second sealing edge is disposed on the periphery of the sample cavity, so that a first groove is formed on one side, close to the second sealing layer, of the middle structure layer, the sample cavity is located in the first groove, and the cover body is covered on the first groove.
Optionally, the first groove includes two opposite lateral walls and is located two the connecting wall between the lateral walls, two the lateral walls are close to the one end of connecting wall is provided with the pinhole respectively, two opposite corners of lid be provided with the round pin axle that the pinhole corresponds to be connected, make the lid is rotatory around the round pin axle in order to open or close the detection box main part.
Optionally, a hook is disposed on a side of the cover body away from the pin shaft, and a clamping hole matched with the hook to lock the cover body and the detection box main body is disposed on the middle structure layer.
Optionally, an elastic sealing element is disposed on a side of the cover body facing the middle structure layer, a through hole is disposed on the cover body to expose the elastic sealing element, and an orthographic projection of the through hole on the middle structure layer is located in the sample cavity.
Optionally, a sealing ring is disposed on a side of the elastic sealing member facing the middle structural layer, and when the cover body covers the detection box main body, the sealing ring is pressed on an edge of the sample cavity, or the sealing ring is enclosed around the sample cavity.
Optionally, a plurality of hanging buckles are arranged on one side, facing the middle structure layer, of the cover body, a plurality of hanging holes are formed in the periphery of the sealing ring, and the hanging buckles are in interference fit with the hanging holes to connect the cover body and the elastic sealing member.
Optionally, the main runner is teardrop, the main runner has relative first end and second end, the branch runner set up in first end, by first end extremely the second end, the cross-sectional area of main runner in being on a parallel with the direction of sealing layer increases gradually.
Optionally, the plugging piece is made of paraffin.
The embodiment of the invention also provides a detection device, which comprises:
in the microfluidic chip, the detection box main body comprises an intermediate structure layer and a first sealing layer positioned on one side, far away from the cover body, of the intermediate structure layer, an elastic sealing element is arranged on one side, facing the intermediate structure layer, of the cover body, a through hole is formed in the cover body to expose the elastic sealing element, and orthographic projection of the through hole on the intermediate structure layer is positioned in the sample cavity;
a heating plate located on a side of the cartridge body remote from the cover, the heating plate configured to heat to melt the closure;
the ejector rod is configured to pass through the through hole on the cover body to press the elastic sealing piece, so that the extraction reagent in the sample cavity flows into the first runner through the runner port and enters the reaction cavity.
The beneficial effects of the invention are as follows: the first flow channel used for communicating the sample cavity and the reaction cavity comprises a main flow channel and a branch flow channel, and a plurality of wetting columns are arranged in the main flow channel at intervals, and the liquid blocking piece flows in a direction away from the branch flow channel by utilizing capillary action, so that the problem that the flow channel is blocked to influence the flow direction of a reagent to the reaction cavity is solved.
Drawings
Fig. 1 shows a schematic structural diagram of a microfluidic chip according to an embodiment of the present invention;
fig. 2 shows a schematic structural diagram of a microfluidic chip according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of a first flow channel according to an embodiment of the invention;
FIG. 4 is a schematic diagram showing a second structure of the first flow channel according to the embodiment of the invention;
FIG. 5 shows a third schematic structural view of a first flow channel according to an embodiment of the present invention;
FIG. 6 is a schematic diagram showing connection between a reaction chamber and a second flow channel in an embodiment of the present invention;
fig. 7 shows a schematic structural diagram of a microfluidic chip according to an embodiment of the present invention;
FIG. 8 shows an exploded view of a cover in an embodiment of the invention;
FIG. 9 is a schematic view showing the structure of a cartridge main body in the embodiment of the present invention;
FIG. 10 is a schematic view showing a state before the ejector pin is pushed down in the embodiment of the present invention;
FIG. 11 is a schematic view showing a state of the ejector pin after being pushed down in the embodiment of the present invention;
FIG. 12 shows a block diagram of a reagent flow path in an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. It will be apparent that the described embodiments are some, but not all, embodiments of the invention. All other embodiments, which are obtained by a person skilled in the art based on the described embodiments of the invention, fall within the scope of protection of the invention.
In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Referring to fig. 1-8, the present embodiment provides a microfluidic chip, which includes a cartridge body 2 and a cover 1, wherein a sample chamber 21 for accommodating an extraction reagent and a reaction chamber 26 located at one side of the sample chamber 21 are disposed in the cartridge body 2;
a flow channel opening 25 is arranged on one side of the sample cavity 21 far away from the cover body 1, a sealing piece is arranged in the flow channel opening 25 for sealing, and the sealing piece is configured to open the flow channel opening 25 under preset conditions;
the bottom of the detection box main body 2 is provided with a first flow channel, the first flow channel is used for communicating the flow channel opening 25 and the reaction cavity 26, the detection box main body 2 comprises an intermediate structure layer 22 and a first sealing layer 23 which is positioned on one side of the intermediate structure layer 22 away from the cover body 1, the sample cavity 21 and the reaction cavity 26 are positioned on the intermediate structure layer 22, a first sealing edge 221 is arranged on the bottom of the intermediate structure layer 22 towards the direction away from the cover body 1, and a preset groove is arranged on the first sealing edge 221 or on the first sealing layer 23, so that the intermediate structure layer 22 and the first sealing layer 23 are surrounded to form the first flow channel between the first sealing edge 221 and the first sealing layer 23.
In one embodiment, the first sealing edge 221 is integrally injection molded with the intermediate structural layer 22.
In this embodiment, the first flow channel is sealed between the first sealing edge 221 and the first sealing layer 23, so as to prevent the extraction reagent in the sample chamber 21 from being contaminated by aerosol during the process of entering the reaction chamber through the first flow channel.
In an exemplary embodiment, the microfluidic chip further includes:
an air cavity 29 provided in the intermediate structural layer 22;
and a second flow passage 27, wherein the second flow passage 27 is used for communicating the air cavity 29 and the reaction cavity 26.
After the flow channel opening 25 is opened, the extraction reagent in the sample chamber 21 enters the reaction chamber 26 through the first flow channel, and the excessive small amount of extraction reagent enters the second flow channel 27 or enters the air chamber 29 through the second flow channel 27.
In an exemplary embodiment, the microfluidic chip further includes a second sealing layer 202 located on a side of the intermediate structure layer near the cover body, and the cover body 1, the second sealing layer 202, and the intermediate structure layer 22 form the second flow channel 27.
In an embodiment, the intermediate structure layer 22 is provided with a second groove, the second sealing layer 202 is an elastic structure and covers the intermediate structure layer 22, a protrusion is disposed on one side of the cover 1 near the intermediate structure layer 22, the shape of the protrusion conforms to the shape of the second groove on the intermediate structure layer 22, when the cover 1 is covered on the intermediate structure layer 22, the second sealing layer 202 deforms, and the protrusion is pressed in the second groove (the width of the protrusion is smaller than the width of the second groove) so as to form the second flow channel 27 with a width conforming to the requirement between the cover 1, the second sealing layer 202 and the intermediate structure layer 22.
In an exemplary embodiment, the microfluidic chip further includes a second sealing layer 202 located on a side of the intermediate structure layer 22 near the cover 1, a second sealing edge 201 is disposed on a side of the intermediate structure layer 22 near the cover 1, and the intermediate structure layer 22 and the second sealing layer 202 are surrounded, so that the second flow channel 27 is formed between the second sealing edge 201 and the second sealing layer 202. In this embodiment, the second flow channel 27 is formed by surrounding the second sealing edge 201 and the second sealing layer 202, grooves with corresponding patterns are provided on the second sealing edge 201 and/or the second sealing layer 202, and the second flow channel 27 is formed after the second flow channel 27 is surrounded by the second sealing edge 201 and the second sealing layer 202.
In one embodiment, the second sealing edge 201 is integrally injection molded with the intermediate structural layer 22.
The middle structure layer 22 is close to the second sealing edge 201 on one side of the cover body 1 and the second sealing layer 202 are in sealing connection, and the first sealing edge 221 on one side of the middle structure layer 22 away from the cover body 1 is in sealing connection with the first sealing layer 23, so that the microfluidic chip forms a full-sealing structure, and pollution of aerosol is effectively prevented.
In the exemplary embodiment, the intermediate structure layer 22 is provided with a plurality of reaction chambers 26, the first flow channel includes a main flow channel 241 communicated with the flow channel ports, and a branch flow channel extending from the main flow channel 241 to the reaction chambers and communicated with the reaction chambers, and the branch flow channel includes a plurality of sub-branch flow channels 242 respectively communicated with the plurality of reaction chambers 26 in a one-to-one correspondence;
the second flow channel 27 comprises a plurality of sub flow channels which are respectively communicated with the reaction chambers 26 in a one-to-one correspondence manner;
the intermediate structure layer 22 comprises a plurality of air cavities 29, and the air cavities 29 are communicated with the sub-runners in a one-to-one correspondence manner.
The intermediate structure layer 22 includes a plurality of air cavities 29, a plurality of air cavities 29 are arranged at intervals along the first direction, and a plurality of air cavities 29 are communicated with a plurality of sub-channels in a one-to-one correspondence manner.
The extracting reagent in the sample chamber 21 is automatically and uniformly divided into multiple paths through the main flow channel 241, enters the corresponding reaction chamber 26 through a plurality of branch flow channels, is re-dissolved in the freeze-dried balls, and the redundant small amount of reagent residues enter the corresponding air chambers 29 through the corresponding sub flow channels.
The number of the reaction chambers 26 and the number of the corresponding air chambers 29 can be set according to actual needs, in this embodiment, the first flow channel, the reaction chambers 26, the second flow channel 27, and the air chambers 29 are hermetically disposed in the detection box main body 2, that is, the air chambers 29 are also in a closed state, and after the sample chambers 21 release the extraction reagent, the sample chambers 21, the reaction chambers 26, and the air chambers 29 are in a constant pressure state, so that the liquid (that is, the extraction reagent) is uniformly distributed in the air chambers 29.
Referring to fig. 12, the number of the air chambers 29 may be set according to actual needs (a plurality of the branch flow channels are in one-to-one correspondence with a plurality of the reaction chambers 26, a plurality of the reaction chambers 26 are in one-to-one correspondence with a plurality of the second flow channels 27, a plurality of the second flow channels 27 are in one-to-one correspondence with a plurality of the air chambers 29), n is greater than or equal to 1, and n is a natural number.
The provision of a plurality of air chambers 29, i.e. a plurality of flow paths, facilitates the uniform distribution of the liquid, and in an embodiment, the volumes of the reaction chambers 26 are the same, the volumes of the air chambers 29 are the same, and the lengths or volumes of the second flow passages 27 are the same, which is more beneficial to the uniform distribution of the liquid.
The number n of air chambers 29 is exemplified by the reagent volume V 1 And the volume V of the reaction chamber 2 Determining that V is required to be satisfied 1 >n*V 2 5, allowing reagents after liquid intakeV occupying at least the volume of the reaction chamber 2 And 5, the liquid inlet stability is higher.
It should be noted that, the number n of the air chambers 29 needs to be set to satisfy that each reaction chamber contains a liquid (i.e. a corresponding extraction reagent) after the liquid is fed, and the ratio of the reagent occupying at least the volume of the reaction chamber after the liquid is fed may be set according to actual needs, and is not limited to 1/5, 1/3, 1/4, etc. of the volume of the reaction chamber.
Particularly, the uniform distribution of the liquid inlet amount of each reaction cavity can be realized by one-time liquid inlet through designing the volume and the length of each branch flow passage and the volume parameters of each air cavity.
In an exemplary embodiment, the volumes of the plurality of reaction chambers 26 are different, the volumes of the plurality of air chambers 29 are different, or the lengths or volumes of the plurality of second flow channels are different, so that a specific ratio of liquid is dispensed.
The specific structures of the air chamber 29, the reaction chamber 26, the second flow channel 27, and the first flow channel may be set according to actual needs.
In the exemplary embodiment, the reaction chambers 26 and the air chambers 29 are respectively located at two opposite sides of the first flow channel in the extending direction, and the intermediate structural layer 22 is provided with a plurality of reaction chambers 26 at intervals along a first direction, where the first direction is perpendicular to the extending direction of the first flow channel;
the second flow path 27 includes a plurality of sub-flow paths respectively communicating with the plurality of reaction chambers 26 in one-to-one correspondence, and the plurality of sub-flow paths are located on at least one side of the sample chamber 21 in the first direction.
With the above-described structure, the length of the cartridge body in the extending direction of the first flow path can be reduced as compared with the case where the reaction chamber 26 and the air chamber 29 are provided on the same side of the first flow path.
In the exemplary embodiment, the extending direction of the reaction chamber 26 is perpendicular to the first direction and parallel to the direction from the first sealing layer 23 to the second sealing layer 202, the reaction chamber 26 is disposed through the intermediate structural layer 22, and in the extending direction of the reaction chamber, an inlet communicating with the first flow channel and an outlet communicating with the second flow channel 27 of the reaction chamber 26 are respectively located at both ends of the reaction chamber 26.
In an exemplary embodiment, the first flow channel includes a main flow channel 241 communicating with the flow channel port 25, and a branch flow channel extending from the main flow channel 241 to the reaction chamber 26 and communicating with the reaction chamber 26, a plurality of wetting columns 2401 disposed at intervals are disposed in the main flow channel 241, and a distance between two adjacent wetting columns 2401 is smaller than a width of a communicating portion between the main flow channel 241 and the branch flow channel, so that the blocking member flows in a direction away from the branch flow channel.
Under preset conditions, the plugging member will flow along the flow channel to the reaction chamber 26 while opening the flow channel opening 25, so that the risk of plugging the flow channel is present, in this embodiment, the structural form of the flow channel is changed, the first flow channel includes the main flow channel 241 and the branch flow channel, the volume of the main flow channel 241 is larger than that of the branch flow channel, in the direction perpendicular to the flowing direction of the reagent, the width of the main flow channel 241 is larger than that of the branch flow channel, a plurality of spaced wetting columns 2401 are disposed in the main flow channel 241, and the distance between two adjacent wetting columns 2401 is smaller than that of the communicating part between the main flow channel 241 and the branch flow channel, so that the liquid plugging member flows in the direction close to the wetting columns 2401, i.e. flows in the direction away from the branch flow channel by capillary action, thereby avoiding the liquid plugging member entering the branch flow channel to plug the branch flow channel.
In an exemplary embodiment, the diameter of the wetting columns 2401 is 1-3mm, and the distance between two adjacent wetting columns 2401 is 1-3mm, so that capillary force wetting effect is realized; in one embodiment, the diameter of the wetting columns 2401 is 2mm, and the distance between two adjacent wetting columns 2401 is 2mm, but not limited thereto.
In an exemplary embodiment, the plugging member is made of paraffin, and the preset condition is that the paraffin is converted from a solid state to a liquid state under a preset temperature environment, but the plugging member is not limited thereto, and the plugging member may also be made of other phase change materials, and the preset temperature needs to be greater than the melting point of the plugging member, so that the plugging member may be converted from a solid state to a liquid state at the preset temperature, and reference numeral 100 in fig. 4 indicates a wax drop formed after the paraffin is melted. For example, the blocking element may also be a PE wax, the predetermined temperature being greater than the melting point of the PE wax. For example, the blocking element may also be an electrorheological fluid, which is solid when energized, liquid when not energized, the preset condition being to shut off the passing current, etc.
Illustratively, the distance between the runner 25 and the adjacent wetting post 2401 may be greater than or equal to the distance between the adjacent wetting posts 2401, so as to facilitate capillary wetting effect, and the melted liquid paraffin preferentially flows in a direction approaching the wetting post 2401 under wetting effect.
In an embodiment, the distance between the runner 25 and the adjacent wetting post 2401 may be greater than the distance between the adjacent wetting posts 2401, the distance between the runner 25 and the adjacent wetting post 2401 may be a first value, the distance between the adjacent wetting posts 2401 may be a second value, and the difference between the first value and the second value may be less than 1mm.
The diameter of the runner 25 is 0.1-5mm, and in one embodiment, the diameter of the runner 25 may be 1-3mm. The depth of the runner 25 is 1mm, paraffin is melted and filled in the runner 25 through self fluidity, the paraffin forms an I-shaped form, the runner 25 is plugged (under the condition of no external force, the paraffin cannot move along the runner).
In an exemplary embodiment, the material of the main body of the detection box may be a common injection molding base material such as PC (polycarbonate), PP (polypropylene), PS (polystyrene), acryl, etc., but not limited thereto.
The material of the wetting column is the same as that of the main body of the detection box, so that the wetting column and the detection and main body are integrally injection molded, and the manufacturing process is simplified, but the method is not limited to the method.
In an exemplary embodiment, the main flow channel 241 includes a first end where the branch flow channel is disposed, and a second end opposite to the first end, the flow channel port 25 is located in a region of the main flow channel 241 near the second end, the wetting columns 2401 are located around the flow channel port 25, or the wetting columns 2401 are located at a side of the flow channel port 25 near the second end, or the main flow channel 241 includes an annular region located at a periphery of the flow channel port 25, the annular region includes a first region facing the branch flow channel, and a second region other than the first region, and the wetting columns 2401 are disposed in the second region.
By adopting the scheme, the distance between the runner port 25 and the branch runner can be increased, the liquid blocking piece is effectively prevented from flowing to the branch runner, and the branch runner is prevented from being blocked.
In an exemplary embodiment, the distance between the flow gate 25 and the wetting column 2401 adjacent thereto may be greater than or equal to the spacing between adjacent wetting columns 2401.
In an exemplary embodiment, the main flow channel 241 is teardrop, the main flow channel 241 has a first end and a second end opposite to each other, the branch flow channel is disposed at the first end, and a cross-sectional area of the main flow channel 241 in a direction parallel to the sealing layer gradually increases from the first end to the second end. The liquid can be caused to flow toward the first end, improving the flow efficiency of the liquid.
Illustratively, the maximum width of the second end of the main flow channel 241 is 8-15mm, the width of the branch flow channel 242 is 0.5-2mm, and the distance from the position of the maximum width of the main flow channel 241 to the starting point of the branch flow channel 242 is 5-10mm, so that the size change rate of the main flow channel is large enough to avoid forming a blockage at the starting point of the branch flow channel 242.
Illustratively, the branched flow channel includes a plurality of sub-branched flow channels 242 corresponding to the reaction chambers 26 one by one, and the sub-branched flow channels 242 are disposed at the first end of the main flow channel 241, in this way, uniform flow of the liquid is achieved, and uniform distribution of the liquid is achieved in cooperation with the arrangement of the air chambers 29.
In this embodiment, the reaction chamber 26 is disposed on a side of the branched flow channel away from the main flow channel 241 in a direction from the main flow channel 241 to the branched flow channel, and the air chamber 29 is disposed on a side of the main flow channel 241 away from the branched flow channel.
Illustratively, the air cavity 29 is disposed on a side of the main flow channel 241 away from the branch flow channel, and the air cavity 29 is located inside the middle structural layer 22, and the microfluidic chip further includes a third flow channel 28, where the third flow channel 28 is disposed between the second flow channel 27 and the air cavity 29, and an extension direction of the third flow channel 28 is parallel to an extension direction of the reaction cavity 26, referring to fig. 12.
In this embodiment, the first flow channel, the reaction chamber 26, the second flow channel 27, and the air chamber 29 are hermetically disposed in the detection box main body 2, and are in a fully sealed state, so as to realize detection without aerosol pollution.
In an exemplary embodiment, the cover 1 is disposed on a side of the second sealing layer 202 remote from the intermediate structure layer 22, and the front projection of the cover 1 on the intermediate structure layer 22 covers the intermediate structure layer. In this embodiment, the area of the cover 1 corresponds to the area of the surface of the intermediate structure layer 22 on the side close to the cover 1, the cover 1 is entirely covered on the intermediate structure layer 22, and since the second seal layer 202 is sealingly provided on the intermediate structure layer 22, only the sample chamber is exposed even when the cover 1 is in the open state, and the second flow path 27 is sealed by the structure formed by the second seal layer 202 and the intermediate structure layer 22.
In an exemplary embodiment, the second sealing edge 201 is disposed on the periphery of the sample cavity 21, the second sealing layer 202 is in a plate structure with a notch, so that a first groove is formed on a side, close to the second sealing layer 202, of the intermediate structure layer 22, the sample cavity is located in the first groove, and the cover 1 is covered on the first groove. In this embodiment, the area of the cover 1 is smaller than the area of the surface of the intermediate structure layer 22 on the side close to the cover 1, the cover 1 is only covered on the first groove formed by the intermediate structure layer 22, and when the cover 1 is opened, the sample cavity in the first groove is exposed.
The second sealing layer 202 is illustratively, but not limited to, a U-shaped structure, and the groove is U-shaped.
Fig. 1 is a schematic diagram of a cover body in a locked state, and fig. 2 is a schematic diagram of a cover body in an open state, in an exemplary embodiment, the groove includes two opposite side walls and a connecting wall between the two side walls, one ends of the two side walls, which are close to the connecting wall, are respectively provided with a pin hole 204, and two opposite corners of the cover body 1 are provided with pin shafts 104 correspondingly connected with the pin holes 204, so that the cover body 1 rotates around the pin shafts 104 to open or close the detection box main body 2.
The cover 1 is provided with a fixed end at one end of the pin 104, the cover 1 further comprises a free end opposite to the fixed end, and the cover 1 can rotate around the pin 104 through the cooperation of the pin 104 and the pin hole 204, so that the detection box main body 2 can be opened or closed.
In an exemplary embodiment, a hook 102 is disposed on a side of the cover 1 away from the pin 104, and a clamping hole 203 that cooperates with the hook 102 to lock the cover 1 and the detection box main body 2 is disposed on the middle structure layer 22.
The hook 102 is made of elastic material, and the hook 102 is pressed down with force to make the hook 102 and the hook hole 203 cooperate to lock the detection box main body 2, when the detection box main body 2 is opened, the cover 1 can be forced in a direction away from the detection box main body 2, so that the hook 102 is deformed to be separated from the hook hole 203.
Illustratively, the hook 102 includes an extending portion perpendicular to the cover 1, and a protrusion located at an end of the extending portion away from the cover 1, where the protrusion is located on one side of the extending portion, the clamping hole 203 includes a groove disposed on one side of the middle structural layer 22 near the cover 1, the groove is used for accommodating the extending portion, and the clamping hole 203 further includes a clamping groove disposed on a side wall of the groove, where the clamping groove may be matched with the protrusion to connect the cover 1 and the detection box main body 2.
The first flow channel is used for communicating the sample cavity 21 with the reaction cavity 26, in a non-detection state, the cover body 1 is covered on the detection box main body 2, the first flow channel is plugged by a plugging piece, the extraction reagent contained in the sample cavity 21 is stored in the sample cavity 21, and when in use, the cover body 1 is opened, the sampler 10 stretches into the sample cavity 21 and is immersed in the extraction reagent, a sample is released, and the cover body 1 is covered, so that the operation flow of detecting and sample adding is completed.
In an exemplary embodiment, the elastic sealing member 12 is disposed on the side of the cover 1 facing the middle structure layer 22, the through hole 101 is disposed on the cover 1 to expose the elastic sealing member 12, and the orthographic projection of the through hole 101 on the middle structure layer 22 is located in the sample cavity 21.
The detecting instrument includes a pressing structure for pressing the elastic sealing member 12 through the through hole 101 so that the elastic sealing member 12 is deformed, generating a positive pressure driving force so that the extraction reagent in the sample chamber 21 flows to the reaction chamber 26 through the first flow path.
In an exemplary embodiment, a sealing ring 122 is disposed on a side of the elastic sealing member 12 facing the middle structural layer 22, and when the cover 1 is covered on the detection box main body 2, the sealing ring 122 is pressed onto an edge of the sample cavity 21, or the sealing ring 122 is enclosed around the sample cavity 21.
Illustratively, a receiving groove may be disposed on the top of the sample chamber 21 or around the sample chamber 21 to embed the sealing ring 122, so as to ensure the sealing effect.
For example, the elastic sealing member 12 and the sealing ring 122 may be integrally formed, and the elastic sealing member 12 may be made of silicone rubber, TPE (Thermoplastic Elastomer ), TPU (Thermoplastic polyurethanes, thermoplastic polyurethane elastomer), or the like, but is not limited thereto.
In an exemplary embodiment, a plurality of hanging buckles 103 are disposed on a side of the cover body 1 facing the middle structural layer 22, a plurality of hanging holes 121 located on the periphery of the sealing ring 122 are disposed on the elastic sealing member 12, and a plurality of hanging buckles 103 are in interference fit with a plurality of hanging holes 121 to connect the cover body 1 and the elastic sealing member 12.
The specific structural form of the hanging buckle 103 may be various, so long as the hanging buckle can be matched with the hanging hole 121 to realize the connection between the cover body 1 and the elastic sealing member 12, for example, the hanging buckle 103 may be a column structure, the radial sectional area of the column structure is greater than the aperture of the hanging hole 121, for example, the hanging buckle 103 may be an i-shaped structure, and the hanging buckle 103 passes through the hanging hole 121, so that the elastic sealing member 12 is clamped in the middle part of the i-shaped structure.
With reference to fig. 9 and 10, an embodiment of the present invention provides a detection apparatus, including:
the detection device and the microfluidic chip, the detection box main body comprises an intermediate structure layer 22 and a first sealing layer positioned on one side of the intermediate structure layer 22 away from the cover body, an elastic sealing element 12 is arranged on one side of the cover body 1 facing the intermediate structure layer 22, a through hole 101 is arranged on the cover body 1 to expose the elastic sealing element 12, and the orthographic projection of the through hole 101 on the intermediate structure layer 22 is positioned in the sample cavity 21;
the detection apparatus includes:
a heating plate 200 located at a side of the cartridge body 2 remote from the cover 1, the heating plate 200 being configured to heat to melt the stopper;
a push rod 100 is configured to pass through the through hole 101 of the cover 1 to press the elastic sealing member 12, so that the extraction reagent 300 in the sample chamber 21 flows into the first flow channel through the flow channel port 25 and into the reaction chamber 26.
The heating plate 200 is heated to a preset temperature, so that the plugging piece is melted, the runner port 25 is opened, the ejector rod 100 can extend into the through hole 101 on the cover body 1, the elastic sealing piece 12 is extruded, the elastic sealing piece 12 is deformed, and a forward driving force is applied to the sample cavity 21, so that the extraction reagent in the sample cavity 21 enters the reaction cavity 26 through the first runner.
When the ejector rod 100 is pressed down, the heating plate can be triggered simultaneously to heat, so that the operation flow is simplified, the operation times of a user are reduced, and forgetting is not easy (for example, if an operation mode of heating firstly and then pressing down the ejector rod 100 is adopted, the operation of pressing down the ejector rod 100 is easy to forget after heating); and the phase change efficiency of the plugging piece can be improved by firstly extruding and then heating the sample cavity.
In an exemplary embodiment, after the microfluidic chip is placed in the detection device (the detection device includes the ejector pin and the heating plate), the temperature rise-heat preservation-wax melting is triggered, and the user presses down the ejector pin after waiting for a period of time or directly presses down the ejector pin after the microfluidic chip is placed in the detection device (the detection device includes the ejector pin and the heating plate).
The following describes a specific flow of detection by the detection device in this embodiment:
after the user samples, the sample cavity 21 is added, and the sample cavity 21 comprises an extraction reagent for releasing a sample;
and placing the microfluidic chip added with the sample into detection equipment, pressing down the ejector rod 100, and automatically heating the heating plate 200, wherein when the heating plate is heated to a preset temperature, the blocking piece (in the embodiment, the blocking piece adopts paraffin) melts and flows into the main flow channel 241.
It should be noted that, a plurality of wetting columns 2401 are disposed in the main flow channel 241 at intervals, and the distance between two adjacent wetting columns 2401 is smaller than the width of the connecting part between the main flow channel 241 and the branch flow channel, so that the plugging member in liquid state flows in the direction close to the wetting columns 2401, i.e. in the direction away from the branch flow channel by capillary action, and further, the plugging member in liquid state is prevented from entering the branch flow channel to plug the branch flow channel.
In this embodiment, the main flow channel 241 is teardrop, the main flow channel 241 has a first end and a second end opposite to each other, the branch flow channel is disposed at the first end, the wetting column 2401 is located in a region of the main flow channel 241 near the second end, and the flow channel opening 25 is located in a region near the second end, from the first end to the second end, and a cross-sectional area of the main flow channel 241 in a direction parallel to the sealing layer gradually increases. The liquid can be promoted to flow to the first end, and the flow passage efficiency of the liquid is improved. The main flow channel 241 has a symmetrical structure in a third direction, and the third direction is perpendicular to the direction from the first end to the second end, so that the effect of promoting the extraction reagent to flow into the branch flow channel can be achieved.
It should be noted that, in this embodiment, the branching flow channel includes a plurality of sub-branching flow channels 242, each sub-branching flow channel 242 is correspondingly connected to a reaction chamber, the main flow channel 241 includes a center line extending from the first end to the second end, and along the third direction, the plurality of sub-branching flow channels 242 are symmetrically disposed on two sides of the center line, so as to facilitate uniform distribution of the liquid (i.e. the extraction reagent combined with the sample).
The sample-bound extraction reagent in the sample chamber 21 flows into the corresponding reaction chamber 26 (the reaction chamber 26 includes the lyophilized PCR reagent) through the plurality of sub-branch flow passages 242, the redundant reagent enters the air chamber 29 (or remains in the second flow passage 27) through the second flow passage 27, and the detection device performs a PCR reaction by heating the reconstituted and lyophilized PCR reagent to perform nucleic acid detection.
In this embodiment, the first flow channel, the reaction cavity 26, the second flow channel 27, and the air cavity 29 are hermetically disposed in the detection box main body 2, that is, the air cavity 29 is also in a closed state, and after the sample cavity 21 releases the extraction reagent, the sample cavity 21, the reaction cavities 26, and the air cavities 29 are in a constant pressure state, so that the liquid (that is, the extraction reagent) is uniformly distributed in the air cavities 29.
In one embodiment, the volumes of the reaction chambers 26 are different, the volumes of the air chambers 29 are different, or the lengths or volumes of the second flow channels are different, so that a specific ratio of the liquid is achieved.
It is to be understood that the above embodiments are merely illustrative of the application of the principles of the present invention, but not in limitation thereof. Various modifications and improvements may be made by those skilled in the art without departing from the spirit and substance of the invention, and are also considered to be within the scope of the invention.

Claims (18)

1. The microfluidic chip is characterized by comprising a detection box main body and a cover body, wherein a sample cavity for containing an extraction reagent and a reaction cavity positioned at one side of the sample cavity are arranged in the detection box main body;
a flow channel opening is formed in one side, far away from the cover body, of the sample cavity, a plugging piece is arranged in the flow channel opening for sealing, and the plugging piece is configured to open the flow channel opening under preset conditions;
the bottom of detecting the box main part is provided with first runner, first runner is used for the intercommunication runner mouth with the reaction chamber, detect the box main part including intermediate structure layer and be located intermediate structure layer is kept away from the first sealing layer of one side of lid, the sample chamber with the reaction chamber is located intermediate structure layer, intermediate structure layer's bottom is to keeping away from the direction of lid is equipped with first sealing edge, first sealing edge or be provided with preset groove on the first sealing layer, make intermediate structure layer with first sealing layer surrounds first sealing layer first sealing edge with form between the first sealing layer first runner.
2. The microfluidic chip according to claim 1, further comprising:
the air cavity is arranged on the middle structural layer;
and the second flow passage is used for communicating the air cavity and the reaction cavity.
3. The microfluidic chip of claim 2, further comprising a second sealing layer on a side of the intermediate structural layer adjacent to the cover, the second sealing layer, and the intermediate structural layer enclosing the second flow channel.
4. The microfluidic chip according to claim 2, wherein,
the sealing structure comprises a cover body and is characterized by further comprising a second sealing layer which is positioned on one side, close to the cover body, of the middle structure layer, wherein a second sealing edge is arranged on one side, close to the cover body, of the middle structure layer, and the middle structure layer is surrounded with the second sealing layer, so that a second flow channel is formed between the second sealing edge and the second sealing layer.
5. The microfluidic chip according to claim 2, wherein a plurality of the reaction chambers are provided on the intermediate structural layer;
the second flow channel comprises a plurality of sub flow channels which are respectively communicated with the reaction cavities in a one-to-one correspondence manner;
the middle structure layer comprises a plurality of air cavities, and the air cavities are communicated with the sub-runners in a one-to-one correspondence mode.
6. The microfluidic chip according to claim 1 or 5, wherein the first flow channel comprises a main flow channel communicated with the flow channel port and a branch flow channel extending from the main flow channel to the reaction cavity and communicated with the reaction cavity, a plurality of wetting columns arranged at intervals are arranged in the main flow channel, and the distance between two adjacent wetting columns is smaller than the width of the communicating part of the main flow channel and the branch flow channel, so that the blocking piece flows in a direction far away from the branch flow channel.
7. The microfluidic chip according to claim 6, wherein the diameter of the wetting columns is 1-3mm, and the distance between two adjacent wetting columns is 1-3mm.
8. The microfluidic chip according to claim 6, wherein a maximum width of the end of the main flow channel away from the branch flow channel is 8-15mm, and a width of the branch flow channel is 0.5-2mm.
9. The microfluidic chip according to claim 4, wherein the cover is covered on a side of the second sealing layer away from the intermediate structure layer.
10. The microfluidic chip according to claim 4, wherein the second sealing edge is disposed at the periphery of the sample cavity, so that a first groove is formed on a side, close to the second sealing layer, of the intermediate structure layer, the sample cavity is located in the first groove, and the cover body is covered on the first groove.
11. The microfluidic chip according to claim 10, wherein the first groove comprises two opposite side walls and a connecting wall between the two side walls, pin holes are respectively formed at one ends of the two side walls, which are close to the connecting wall, and pin shafts correspondingly connected with the pin holes are arranged at two opposite corners of the cover body, so that the cover body rotates around the pin shafts to open or close the detection box main body.
12. The microfluidic chip according to claim 11, wherein a hook is provided on a side of the cover body away from the pin shaft, and a clamping hole matched with the hook to lock the cover body and the detection box main body is provided on the intermediate structure layer.
13. The microfluidic chip according to claim 11, wherein an elastic sealing member is provided on a side of the cover body facing the intermediate structure layer, a through hole is provided on the cover body to expose the elastic sealing member, and an orthographic projection of the through hole on the intermediate structure layer is located in the sample cavity.
14. The microfluidic chip according to claim 13, wherein a sealing ring is disposed on a side of the elastic sealing member facing the middle structural layer, and the sealing ring is pressed onto an edge of the sample cavity or the sealing ring is surrounded around the sample cavity when the cover body is covered on the detection box main body.
15. The microfluidic chip according to claim 14, wherein a plurality of hanging buckles are arranged on one side of the cover body facing the middle structure layer, a plurality of hanging holes are arranged on the elastic sealing element and are positioned on the periphery of the sealing ring, and the hanging buckles are in interference fit with the hanging holes so as to connect the cover body and the elastic sealing element.
16. The microfluidic chip according to claim 6, wherein the main flow channel is teardrop-shaped, the main flow channel has a first end and a second end opposite to each other, the branch flow channel is disposed at the first end, and a cross-sectional area of the main flow channel in a direction parallel to the sealing layer gradually increases from the first end to the second end.
17. The microfluidic chip according to claim 1, wherein the plug member is made of paraffin.
18. A detection apparatus, characterized by comprising:
the microfluidic chip of any one of claims 1-17, the cartridge body comprising an intermediate structural layer and a first sealing layer on a side of the intermediate structural layer remote from the cover, and the cover being provided with an elastic seal on a side facing the intermediate structural layer, a through hole being provided in the cover to expose the elastic seal, and an orthographic projection of the through hole on the intermediate structural layer being located in the sample cavity;
a heating plate located on a side of the cartridge body remote from the cover, the heating plate configured to heat to melt the closure;
the ejector rod is configured to pass through the through hole on the cover body to press the elastic sealing piece, so that the extraction reagent in the sample cavity flows into the first runner through the runner port and enters the reaction cavity.
CN202211209477.1A 2022-09-30 2022-09-30 Microfluidic chip and detection device Pending CN116966940A (en)

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Application Number Priority Date Filing Date Title
CN202211209477.1A CN116966940A (en) 2022-09-30 2022-09-30 Microfluidic chip and detection device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202211209477.1A CN116966940A (en) 2022-09-30 2022-09-30 Microfluidic chip and detection device

Publications (1)

Publication Number Publication Date
CN116966940A true CN116966940A (en) 2023-10-31

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Country Link
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117504797A (en) * 2023-12-29 2024-02-06 常州新一产生命科技有限公司 Synthetic column structure and biochemical reaction equipment

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117504797A (en) * 2023-12-29 2024-02-06 常州新一产生命科技有限公司 Synthetic column structure and biochemical reaction equipment

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