CN217526452U - Purification column - Google Patents
Purification column Download PDFInfo
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- CN217526452U CN217526452U CN202220404601.9U CN202220404601U CN217526452U CN 217526452 U CN217526452 U CN 217526452U CN 202220404601 U CN202220404601 U CN 202220404601U CN 217526452 U CN217526452 U CN 217526452U
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- cylinder
- wall
- purification
- purification column
- adsorption film
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a purification column, which comprises a column body, an adsorption film and a plug, wherein the column body is a cylinder body with an opening at the top and a sealed bottom, a liquid outlet is arranged at the bottom end of the cylinder body, and the liquid outlet is communicated with the inner cavity of the cylinder body; the adsorption film is laid on the inner bottom surface of the cylinder and covers the liquid outlet; the plug is arranged on the adsorption film and used for plugging the bottom end of the cylinder body; the plug comprises a supporting wall and a covering film which are connected together, the upper end and the lower end of the supporting wall are open, and the covering film is connected to the end face, far away from the adsorption film, of the supporting wall and covers the end opening of the supporting wall. The utility model discloses a purification post can regard as the test tube to use and can regard as purification post to use again, need not prepare the test tube consumptive material alone, has both practiced thrift the test tube, has practiced thrift the time that solution shifted to purification post from the test tube again, has practiced thrift the cost, has improved efficiency.
Description
Technical Field
The utility model discloses experiment container technical field, in particular to purification post.
Background
In biochemical reactions, a purification column is often used, and in nucleic acid extraction or purification by a column method, a nucleic acid purification column is used. The nucleic acid adsorption membrane is arranged at the position of the liquid outlet of the nucleic acid purification column, when the solution is transferred into the nucleic acid purification column, the nucleic acid is adsorbed by the nucleic acid adsorption membrane, and substances except the nucleic acid flow out of the liquid outlet after passing through the nucleic acid adsorption membrane.
In the conventional column-type nucleic acid purification, a sample of a nucleic acid solution to be purified, such as various cell or virus solutions, is transferred into a test tube, a cell lysis solution is added into the test tube to lyse and break the cells or viruses in the sample, the nucleic acid in the cells is released into the solution, the solution in the test tube is transferred into a nucleic acid purification column by a liquid transfer component such as a liquid transfer device or a liquid transfer tip, the solution in the nucleic acid purification column flows downwards by centrifugation or negative pressure, the nucleic acid in the solution is adsorbed by a nucleic acid adsorption membrane in the downward flow process, substances except the nucleic acid flow away through the nucleic acid adsorption membrane, and the nucleic acid adsorption membrane is washed and eluted to obtain the purified nucleic acid molecules. The current column method nucleic acid purification process needs to use the consumptive material of test tube, and it needs to waste a large amount of time to transfer the solution in the test tube to the nucleic acid purification post simultaneously, needs to use the utensil of transferring the solution simultaneously, waste time and cost.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model provides a purification post can regard as the test tube to use and can regard as purification post to use again, need not prepare the test tube consumptive material alone, has both practiced thrift the test tube, has practiced thrift the time that solution shifted to purification post from the test tube again, has practiced thrift the cost, has improved efficiency.
In order to achieve the above purpose, the utility model provides a following technical scheme:
a purification column comprising:
the cylinder is a cylinder with an opening at the top and a sealed bottom, a liquid outlet is formed in the bottom end of the cylinder, and the liquid outlet is communicated with the inner cavity of the cylinder;
the adsorption film is laid on the inner bottom surface of the cylinder and covers the liquid outlet;
the plug is arranged on the adsorption film and used for plugging the bottom end of the cylinder body;
the plug comprises a supporting wall and a covering film which are connected together, the upper end and the lower end of the supporting wall are open, and the covering film is connected to the end face, far away from the adsorption film, of the supporting wall and covers the end opening of the supporting wall.
Optionally, the outer wall surface of the supporting wall is conformal with the inner surface of the cylinder, and the size of the outer wall surface of the supporting wall is corresponding to the size of the inner surface of the cylinder.
Optionally, the adsorption film covers the inner bottom surface of the barrel, and the adsorption film is arranged corresponding to the inner bottom surface of the barrel.
Optionally, the cover film has a thickness of less than 0.3mm.
Optionally, the height of the support wall is 2-5mm.
Optionally, the cylinder is a cylindrical barrel.
Optionally, the support wall is a cylindrical wall.
Optionally, the support wall has a wall thickness of 2-4mm.
Optionally, the support wall is made of plastic, silica gel or nylon.
Optionally, the cover film is made of plastic, silica gel or nylon;
the adsorption membrane is a nucleic acid adsorption membrane or other purification membranes.
According to the above technical scheme, the utility model provides a purification post sets up the stopper through the bottom at the cylinder, and the liquid outlet that the shutoff is located the cylinder bottom avoids the liquid in the cylinder to flow out, forms the container that open-top bottom sealed, plays the effect that temporarily hinders the solution downflow in the purification post, makes the purification post act as the test tube and uses. When the covering membrane is punctured, the solution in the purification column flows downwards through the punctured holes on the covering membrane, so that the purification column returns to the function of the column. Through shutoff stopper on the adsorption film, can make the purification post can regard as the test tube to use and can regard as the purification post to use again, need not prepare the test tube consumptive material alone, both practiced thrift the test tube, practiced thrift the time that solution shifted from the test tube to the purification post again, practiced thrift the cost, improved experimental efficiency. And simultaneously, the utility model discloses a purification post simple structure makes and uses easily, and current purification post does not need the institutional advancement only to need to be filled in the stopper and can use, makes the purified cost reduction of biochemical molecule, and purification efficiency improves.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic structural diagram of a purification column provided in an embodiment of the present invention;
fig. 2 is a schematic cross-sectional view of a purification column provided in an embodiment of the present invention along an axial direction;
fig. 3 is a schematic perspective view of a stopper according to an embodiment of the present invention;
fig. 4 is a schematic cross-sectional view of a plug according to an embodiment of the present invention;
fig. 5 is a schematic structural diagram of a column provided in an embodiment of the present invention;
fig. 6 is a schematic structural diagram of a purification column according to an embodiment of the present invention after a cover membrane is punctured;
FIG. 7 is a cross-sectional view of FIG. 6 along the axial direction;
fig. 8 is a schematic perspective view of a punctured cover film of a stopper according to an embodiment of the present invention;
FIG. 9 is a schematic cross-sectional view taken along the axial direction of FIG. 8;
fig. 10 is a schematic cross-sectional view of a purification column according to another embodiment of the present invention.
Wherein:
1. a column body which is provided with a plurality of holes,
101. a liquid outlet, a liquid outlet and a liquid outlet,
2. the plug is arranged on the inner side of the bottle body,
201. a covering film, 2010, a hole, 202, a supporting wall, 203, a plastic ring pad,
3. and (5) adsorbing the film.
Detailed Description
The utility model discloses a purification post can regard as the test tube to use and can regard as purification post to use again, need not prepare the test tube consumptive material alone, has both practiced thrift the test tube, has practiced thrift the time that solution shifted to purification post from the test tube again, has practiced thrift the cost, has improved efficiency.
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Fig. 1 is a schematic structural diagram of a purification column provided in an embodiment of the present invention; fig. 2 is a schematic cross-sectional view of a purification column provided by an embodiment of the present invention along an axial direction; fig. 3 is a schematic perspective view of a stopper according to an embodiment of the present invention; fig. 4 is a schematic cross-sectional view of a stopper according to an embodiment of the present invention along an axial direction; fig. 5 is a schematic structural diagram of a column according to an embodiment of the present invention;
fig. 6 is a schematic structural diagram of a purification column according to an embodiment of the present invention after a cover membrane is punctured;
FIG. 7 is a schematic cross-sectional view taken along the axial direction of FIG. 6; fig. 8 is a schematic perspective view of a punctured cover film of a stopper according to an embodiment of the present invention; fig. 9 is a schematic cross-sectional view of a punctured cover film of a stopper according to an embodiment of the present invention; fig. 10 is a schematic cross-sectional view of a purification column according to another embodiment of the present invention.
Referring to fig. 1 to 5, the purification column of the present invention includes a column body 1, an adsorption film 3 and a plug 2. Cylinder 1 is the barrel that open-top bottom sealed, the bottom of barrel is provided with liquid outlet 101, liquid outlet 101 with the inner chamber intercommunication of barrel is used for discharging liquid in the barrel. The adsorption film 3 is laid on the inner bottom surface of the cylinder body and covers the liquid outlet 101, so that the solution in the cylinder body flows out through the liquid outlet 101 after passing through the adsorption film 3. The plug 2 is disposed on the adsorption film 3, and specifically, the plug 2 is disposed on the upper surface of the adsorption film 3.
Wherein, stopper 2 is used for shutoff cylinder 1's bottom end face for cylinder 1 forms the bottom closed barrel. The plug 2 comprises a support wall 202 and a covering membrane 201 joined together, as shown in figures 3 and 4. The upper end and the lower end of the supporting wall 202 are open, the covering film 201 is connected to the end surface of the supporting wall 202 far away from the adsorption film 3, and the covering film 201 covers the end opening of the supporting wall 202, so that the column body 1 is isolated from the liquid outlet 101 under the condition that the covering film 201 is not punctured, and the bottom end of the column body is closed.
The utility model discloses a purification post sets up stopper 2 through the bottom at cylinder 1, and the liquid outlet 101 that the shutoff is located cylinder 1 bottom avoids the liquid outflow in cylinder 1, forms the container that the open-top bottom sealed, plays the effect that temporarily hinders the solution downflow in the purification post, makes the purification post act as the test tube and uses. When the cover film 201 is punctured, as shown in fig. 6 to 9, the solution in the purification column flows down through the punctured holes 2010 in the cover film 201, and the purification column returns to the function of the column. Through shutoff stopper 2 on adsorption film 3, can make the purification post can regard as the test tube to use and can regard as the purification post to use again, need not prepare the test tube consumptive material alone, both practiced thrift the test tube, practiced thrift the time that solution shifted to the purification post from the test tube again, practiced thrift the cost, improved the experimental efficiency. And simultaneously, the utility model discloses a purification post simple structure makes and uses easily, and current purification post does not need the institutional advancement only to need to plug into stopper 2 and can use, makes the purified cost reduction of biochemical molecule, has improved purification efficiency.
In order to realize a better plugging effect, the outer wall surface of the supporting wall 202 and the inner surface of the cylinder are arranged in a conformal manner, and the size of the outer wall surface of the supporting wall 202 and the size of the inner surface of the cylinder are arranged correspondingly, so that the sealing performance of the purification column used as a test tube is improved, and the solution in the cylinder 1 is prevented from flowing out through a gap between the outer wall surface of the supporting wall 202 and the inner surface of the cylinder.
Specifically, the adsorption film 3 covers the inner bottom surface of the barrel, and the adsorption film 3 is arranged corresponding to the inner bottom surface of the barrel, wherein the corresponding arrangement means that the outer side wall of the adsorption film 3 is matched with the inner side wall of the barrel, and the shape and the size are the same.
To facilitate the piercing, the cover film 201 is a thin film-like structure, and the thickness of the cover film 201 is less than 0.3mm.
In order to avoid puncturing the absorbent membrane 3 when puncturing the cover membrane 201, the height of the support wall 202 is larger than 2mm, in particular 2-5mm. When the cover film 201 on the upper end surface of the plug 2 is pierced by a sharp object after the plug 2 is inserted into the column 1 of the purification column, as shown in fig. 8 and 9, the height of the support wall 202 prevents the sharp object from piercing the adsorption film 3 at the lower end surface of the plug 2.
In one embodiment, the column 1 is a cylindrical cylinder and correspondingly, the support wall 202 is a cylindrical wall.
In order to increase the holding capacity of the plug 2, the wall thickness of the holding wall 202 is greater than 2mm, in particular, the wall thickness of the holding wall 202 is 2-4mm.
Further, the supporting wall 202 is made of plastic, silica gel or nylon, and the cover film 201 is made of plastic, silica gel or nylon. Wherein, the adsorption membrane 3 is a nucleic acid adsorption membrane or other purification membranes.
In one embodiment, the thickness of the cover film 201 is 0.05mm to facilitate puncturing, and the support wall 202 is a cylindrical structure with a wall thickness of 2.5mm and a height of 2.5mm for the support wall 202.
In order to prevent the end face of the support wall 202 contacting the adsorption film 3 from damaging the adsorption film 3, a plastic ring pad 203 is disposed on the end face of the support wall 202 contacting the adsorption film 3, as shown in fig. 10, the plastic ring pad is disposed corresponding to the end face of the support wall 202 to form a flexible contact surface, and the plastic ring pad 203 can also have a function of clamping the adsorption film 3. A plastic ring pad 203 and a cover film 201 are fixedly attached to both ends of the support wall 202, respectively.
The utility model discloses a purification post fills in the stopper 2 of softwood matter in the bottom of cylinder 1, and stopper 2 chooses for use the softwood matter so that it closely sets up with the inner wall of cylinder 1 after filling in cylinder 1 to with the bottom seal of cylinder 1. Under the condition that the cover film 201 on stopper 2 is not punctured, cylinder 1 uses as the test tube for prepare nucleic acid solution, under the condition that cover film 201 on stopper 2 is punctured, cylinder 1 uses as the purification post to make the nucleic acid purification in-process need not prepare the test tube in addition, practiced thrift this kind of consumptive material of test tube, practiced thrift the transfer time of transferring the nucleic acid solution in the test tube to the purification post simultaneously, save time, improved the efficiency of nucleic acid purification.
In the description of the present solution, it is to be understood that the terms "upper", "lower", "vertical", "inside", "outside", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present solution.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.
In the present specification, the embodiments are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A purification column, comprising:
the cylinder (1) is a cylinder with an opening at the top and a sealed bottom, a liquid outlet (101) is formed in the bottom end of the cylinder, and the liquid outlet (101) is communicated with an inner cavity of the cylinder;
the adsorption film (3) is laid on the inner bottom surface of the barrel and covers the liquid outlet (101);
the plug (2) is arranged on the adsorption film (3) and is used for plugging the bottom end of the cylinder body;
the plug (2) comprises a supporting wall (202) and a covering film (201) which are connected together, the upper end and the lower end of the supporting wall (202) are open, and the covering film (201) is connected to the end face, far away from the adsorption film (3), of the supporting wall (202) and covers the end opening of the supporting wall (202).
2. The purification column according to claim 1, wherein the outer wall surface of the support wall (202) is conformal to the inner surface of the cylinder, and the outer wall surface of the support wall (202) is sized to correspond to the inner surface of the cylinder.
3. The purification column according to claim 1, wherein the adsorption film (3) covers the inner bottom surface of the cylinder, and the adsorption film (3) is disposed corresponding to the inner bottom surface of the cylinder.
4. The purification column according to claim 1, wherein the thickness of the cover membrane (201) is less than 0.3mm.
5. The purification column according to claim 1, wherein the height of the support wall (202) is 2-5mm.
6. Purification column according to claim 1, characterized in that the column (1) is a cylindrical cartridge.
7. The purification column according to any one of claims 1 to 6, wherein the support wall (202) is a cylindrical wall.
8. The purification column according to claim 1, wherein the wall thickness of the support wall (202) is 2-4mm.
9. The purification column according to claim 1, wherein the support wall (202) is made of plastic, silica gel or nylon.
10. The purification column according to claim 1, wherein the covering membrane (201) is made of plastic, silica gel or nylon;
the adsorption film (3) is a nucleic acid adsorption film.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220404601.9U CN217526452U (en) | 2022-02-25 | 2022-02-25 | Purification column |
| PCT/CN2022/135594 WO2023160091A1 (en) | 2022-02-25 | 2022-11-30 | Purification column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220404601.9U CN217526452U (en) | 2022-02-25 | 2022-02-25 | Purification column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217526452U true CN217526452U (en) | 2022-10-04 |
Family
ID=83428479
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220404601.9U Active CN217526452U (en) | 2022-02-25 | 2022-02-25 | Purification column |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN217526452U (en) |
| WO (1) | WO2023160091A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023160091A1 (en) * | 2022-02-25 | 2023-08-31 | 武汉医蒂生物科技有限公司 | Purification column |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1676906A4 (en) * | 2003-10-21 | 2011-03-09 | Fujifilm Corp | Nucleic acid separation purification cartridge and process for producing the same |
| CN106086003A (en) * | 2016-06-17 | 2016-11-09 | 易初丽 | A kind of nucleic acid purification system |
| CN209797999U (en) * | 2018-12-20 | 2019-12-17 | 武汉医蒂生物科技有限公司 | Extraction and purification device for biochemical molecules |
| CN208414378U (en) * | 2018-03-13 | 2019-01-22 | 武汉医蒂生物科技有限公司 | A kind of nucleic acid extraction purification devices |
| WO2019174539A1 (en) * | 2018-03-13 | 2019-09-19 | 武汉医蒂生物科技有限公司 | Nucleic acid extraction and purification device and biochemical molecule extraction and purification device |
| CN214830261U (en) * | 2021-04-23 | 2021-11-23 | 厦门大学附属第一医院 | Anti-pollution biological tissue draws appearance |
| CN217526452U (en) * | 2022-02-25 | 2022-10-04 | 武汉医蒂生物科技有限公司 | Purification column |
-
2022
- 2022-02-25 CN CN202220404601.9U patent/CN217526452U/en active Active
- 2022-11-30 WO PCT/CN2022/135594 patent/WO2023160091A1/en unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023160091A1 (en) * | 2022-02-25 | 2023-08-31 | 武汉医蒂生物科技有限公司 | Purification column |
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| Publication number | Publication date |
|---|---|
| WO2023160091A1 (en) | 2023-08-31 |
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