CN217709405U

CN217709405U - Sealed orifice plate, reagent sealed orifice plate and liquid-transfering system

Info

Publication number: CN217709405U
Application number: CN202221456915.XU
Authority: CN
Inventors: 秦荣
Original assignee: Shanghai Hongshi Medical Technology Co ltd
Current assignee: Shanghai Hongshi Medical Technology Co ltd
Priority date: 2022-06-12
Filing date: 2022-06-12
Publication date: 2022-11-01
Anticipated expiration: 2032-06-12

Abstract

The application discloses sealed orifice plate, reagent sealed orifice plate and liquid-transfering system, this sealed orifice plate includes: the sealing pore plate comprises a sealing pore plate body, a sealing film and an elastic sealing layer, wherein a plurality of pore grooves are formed in the sealing pore plate body, the sealing film covers the top of the sealing pore plate body, and the elastic sealing layer is further arranged on the sealing film. The reagent sealing orifice plate comprises: reagents stored in sealed well plates. The pipetting system comprises a reagent sealing orifice plate and/or a reagent sealing orifice plate, a pipetting needle tube array, a sampling tube and a sampling tube rack. This application can make every hole groove all sealed alone and not communicate each other, and the elasticity sealing layer in this application uses with the cooperation of liquid transfer needle tubing can improve sealed orifice plate, the sealed orifice plate liquid storage leakproofness of reagent and get liquid leakproofness. Because the central axis distance of the sampling tube grooves of the sampling tube rack, the central axis distance of the pipette tubes are arranged and the central axis distance is the same, the operations of cap screwing and cup separation are not needed before nucleic acid extraction is carried out, the distance change of the pipette tubes is not needed, a large amount of time for the nucleic acid extraction process is saved, and a large amount of cost is also saved.

Description

Sealed orifice plate, reagent sealed orifice plate and move liquid system

Technical Field

The application belongs to the technical field of PCR detection, and particularly relates to a sealing orifice plate, a reagent sealing orifice plate and a liquid transfer system.

Background

At present, the requirement of nucleic acid detection is large, and the requirement of time and accuracy are high, but the current PCR (Polymerase Chain Reaction) detection equipment cannot meet the above requirement, and the following problems exist:

for example, in the uncapping process, a sampling tube with a screw cap is generally adopted for the current nucleic acid sampling. Before the sampling tube is transported to a laboratory for sample nucleic acid extraction, the screw cap needs to be unscrewed, and if manual uncovering is adopted, long time and heavy workload are needed. If an automatic instrument is adopted for completion, an automatic cover opener is additionally adopted for completion.

For example, in the cup separation process, the sample in the sampling tube needs to be added to the well plate of the nucleic acid extractor before entering the nucleic acid extractor for nucleic acid extraction. If manual sample adding is adopted, long sample adding time and heavy workload are needed; if an automatic instrument is adopted for completion, a distance-variable liquid transfer device is required for completion, and the price of the current distance-variable liquid transfer device is very high.

In the process of extracting nucleic acid from a sample, a pipette is generally used to sample from an open sampling tube and load the sample into a well plate, and then the sample to be treated or waste liquid is transferred in the open well plate by using the pipette, or magnetic beads having nucleic acid adsorbed thereon are transferred by using a magnetic rod. While the open sampling tube and the open orifice plate themselves present a risk of contamination from each other. During the transfer, the distance between pipettes or between magnetic bars is only a few millimeters, and contamination is also likely to occur. These are all responsible for the test result being "false positive". If heating is performed in the links of cracking, elution and the like, the risk of pollution is further increased.

In the process of adding the sample to the PCR amplification pore plate after the sample nucleic acid is extracted, the central shaft distance of the PCR amplification pore plate is also very small and open, so that the conditions of pollution and false positive are easy to occur.

SUMMERY OF THE UTILITY MODEL

In view of the above-mentioned shortcomings or drawbacks of the prior art, the present application provides a sealed well plate, a reagent sealed well plate and a pipetting system.

In order to solve the technical problem, the application is realized by the following technical scheme:

the application provides a sealed orifice plate, includes: the sealing pore plate comprises a sealing pore plate body, a sealing film and an elastic sealing layer, wherein at least one row and at least one column of pore grooves arranged in an array are arranged on the sealing pore plate body; the sealing membrane is in sealing connection with the top surface of the sealing pore plate body, so that each pore groove is sealed independently and isolated from each other; the elastic sealing layer is arranged on the sealing film in a sealing mode, each hole groove is sealed independently and isolated from each other through the elastic sealing layer, and the elastic sealing layer is made of elastic materials.

Optionally, the sealed orifice plate described above, wherein the resilient sealing layer is in everywhere sealed connection with the bottom adjacent material;

optionally, in the sealed aperture plate, the elastic sealing layer is connected to the bottom adjacent material in a sealing manner above the top surface of the sealed aperture plate body, and no sealing is provided above the aperture groove.

Optionally, the above sealed orifice plate, wherein the elastic sealing layer is not provided with a seal above the center position of the orifice groove, and is connected with the bottom adjacent material in a sealing manner at the rest positions.

Optionally, the sealed orifice plate described above, wherein the center axis distance between adjacent ones of the orifice slots is 9mm.

Optionally, the sealing orifice plate described above, wherein the sealing membrane is provided as at least one sheet, wherein each sealing membrane seals at least one of the orifice grooves.

Optionally, the sealed well plate described above, wherein the resilient sealing layer is provided as at least one sheet, wherein each sheet seals at least one of the wells.

Optionally, in the above sealed orifice plate, the sealing film is hermetically connected to the top surface of the sealed orifice plate body by thermal plastic sealing, bonding, or ultrasonic welding.

Optionally, the aperture plate is sealed as described above, wherein the resilient sealing layer is bonded to the bottom material to form a sealed connection.

The application also provides a reagent sealing orifice plate the sealed storage of sealing orifice plate hole inslot has the same reagent.

Optionally, the reagent-sealed well plate described above, wherein the reagent comprises: lysis solution, magnetic bead solution, washing solution or eluent.

Optionally, the reagent-sealed well plate described above, wherein the reagent comprises: PCR amplification reagents or lyophilized reagents.

The application also provides a pipetting system, comprising: the reagent sealing pore plate and/or the sealing pore plate are/is used for collecting a sampling pipe of a sample, a sampling pipe frame matched with the sampling pipe frame and used, and a pipette needle tube array. The sampling pipe frame is provided with at least one row and at least one column of sampling pipe grooves which are arranged in an array manner. The pipette needle tube array comprises at least one row of pipette needle tubes arranged in an array manner and at least one column of pipette needle tubes arranged in an array manner. The distance between the central shafts of the hole grooves in the sealing hole plate, the distance between the central shafts of the hole grooves in the reagent sealing hole plate, the distance between the central shafts of the sampling tube grooves and the distance between the central shafts of the pipette needle tubes are the same. The sampling tube groove the line number of transfer liquid needle tubing is N, and the line number is M, the sealed orifice plate the line number of hole groove in the sealed orifice plate of reagent is A N, and the line number is B M, and wherein, N, M, A, B are the integer that is greater than 0, transfer liquid needle tubing array be used for to get liquid in the sampling tube, to the sealed orifice plate get liquid in the sealed orifice plate of reagent.

In the prior art, due to the mandatory requirements of medical instruments, the sealing film is usually made of an irreversible material such as an aluminum plastic film, and when the sealing film is punctured by a pipette needle tube, the hole of the sealing film is opened and can not be recovered. Compared with the prior art, the method has the following technical effects:

this application is through the elastic sealing layer of the sealed setting alone in addition on the seal membrane, and the hole groove that this seal membrane hole leads to is sealed by the elastic sealing layer with external possible intercommunication, and the elastic sealing layer also makes every hole groove sealed alone and mutual isolation promptly to ensure that every hole groove all can be sealed alone at any time. And when the elastic sealing layer is pricked out of the hole by a needle and the needle head is drawn out, the elastic sealing layer can automatically rebound and seal the needle hole, so that the use safety and convenience are further improved.

In the present application, the elastic sealing layer is in everywhere sealed connection with the bottom adjacent material; or the elastic sealing layer is connected with the material adjacent to the bottom but not positioned above the hole groove in a sealing mode; alternatively, the resilient sealing layer is sealingly attached to the material adjacent the base but not over the central position of the well. And further, when the liquid transfer needle tube penetrates through the elastic sealing layer and the sealing film, the needle hole can not contact with and leave the material for sealing and fixedly connecting the elastic sealing layer and the sealing film.

In the application, the central shaft distance of the hole grooves in the sealing hole plate, the central shaft distance of the hole grooves in the reagent sealing hole plate, the central shaft distance of the sampling tube grooves and the central shaft distance of the pipette tubes are the same; the row and column arrangement of the pipette needle tubes is the same as that of the sampling tube grooves; the row and column arrangement of the hole grooves in the sealing orifice plate and the reagent sealing orifice plate is the same as or in a multiple relation with the row and column arrangement of the sampling tube grooves and the pipette tubes, so that the capping and cup separating operation is not needed before the sample nucleic acid extraction, the distance change of the pipette tubes is also not needed, a large amount of sample nucleic acid extraction process time is saved, and a large amount of equipment cost and time cost are also saved.

Drawings

Other features, objects and advantages of the present application will become more apparent upon reading of the following detailed description of non-limiting embodiments thereof, made with reference to the accompanying drawings in which:

FIG. 1: a perspective view of an embodiment of the present application sealing orifice plate;

FIG. 2: in an embodiment of the present application, a first perspective view of a sealing orifice plate body;

FIG. 3: a second perspective view of the sealing orifice plate body in the embodiment of the present application;

FIG. 4: in an embodiment of the present application, a first connection mode between the sealing film and the elastic sealing layer is shown in the figure;

FIG. 5: a second schematic view of the connection between the sealing film and the elastic sealing layer in the embodiment of the present application;

FIG. 6: in an embodiment of the present application, a connection mode between the sealing film and the elastic sealing layer is schematically illustrated in a third embodiment.

Detailed Description

The technical solutions in the embodiments of the present application will be described clearly and completely with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only some embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

In one embodiment of the present application, as shown in fig. 1-3, a sealing orifice plate 30 comprises: sealed orifice plate body 31, seal membrane 32 and elastic sealing layer 33, be equipped with the hole groove 311 that the array of at least one line, at least one row set up on the sealed orifice plate body 31, seal membrane 32 cover in sealed orifice plate body 31 top, still be equipped with on the seal membrane 32 elastic sealing layer 33. In the embodiment, the sealing film 32 and the elastic sealing layer 33 are arranged on the sealing orifice plate body 31, so that each of the orifice grooves 311 is individually sealed without being communicated with each other, and the elastic sealing layer also enables each of the orifice grooves to be individually sealed and isolated from each other, thereby improving the sealing performance and the use safety of the sealing orifice plate 30, and the elastic sealing layer is made of an elastic material, so that the elastic sealing layer 33 can automatically rebound and seal the orifice when being pricked by a needle and the needle is drawn out, thereby further improving the use safety and the convenience.

Wherein, the central axis distance D of the hole slots 311 adjacently arranged is preferably equal to 9mm, as shown in fig. 2.

As shown in fig. 1, the hole slots 311 are arranged in an array of at least one row and at least one column. Further preferably, the distance between the central axes of the adjacent hole slots 311 is the same as the distance between the central axes of the sample tube slots and the pipette tubes in the sample tube rack described below. Specifically, when the number of rows of the sampling tube slot and the pipette needle tube is N and the number of columns is M, the number of rows of the well 311 is a times of N and the number of columns is B times of M, where N, M, a, and B are all integers greater than 0.

Each of the hole grooves 311 is defined by criss-cross partition walls including the outermost peripheral skirt of the sealing orifice plate body 31. In this embodiment, the partition wall may be integrally injection-molded with the sealing orifice plate body 31. The top of each of the hole recesses 311 is sealed with a sealing film 32 and an elastic sealing layer 33.

The sealing film 32 is provided in a sealing manner with the partition wall of the sealing orifice plate body 31, so that each of the orifice grooves 311 is sealed individually. The sealing film 32 is provided as at least one sheet, wherein each sealing film 32 seals at least one of the hole grooves 311 of the sealing orifice plate body 31. Specifically, the sealing film 32 covers the top of the sealing orifice plate body 31, and may be a single film covering and sealing all the orifice grooves 311 of the sealing orifice plate body 31, or may be multiple films, each film covering and sealing at least one orifice groove 311 of the sealing orifice plate body 31. For example, for a 96-well plate, when two sealing membranes are used, each sealing membrane seals 48 wells; when the number of the sealing films is 96, each sealing film seals 1 hole groove.

The sealing method according to the present embodiment includes, but is not limited to: the sealing film 32 is hermetically connected with the partition wall of the sealing orifice plate body 31 through thermal plastic packaging, bonding or ultrasonic welding.

The sealing film 32 is preferably made of a plastic-aluminum film.

In this embodiment, an elastic sealing layer 33 is further disposed on the sealing film 32, the elastic sealing layer 33 is made of a material which can automatically rebound and seal the needle hole when the needle is pricked and the needle is withdrawn, for example, the elastic sealing layer 33 is made of rubber or silicone.

The elastic sealing layer 33 is hermetically connected with the adjacent material at the bottom; alternatively, the elastic sealing layer 33 is hermetically connected with the material adjacent to the bottom but not above the hole slot 311; alternatively, the resilient sealing layer 33 is sealingly attached to the material adjacent the bottom but not over the center of the bore 311. Further, when the pipette needle penetrates through the elastic sealing layer 33 and the sealing film 32, the pinhole does not contact and the material for sealing and fixing between the elastic sealing layer 33 and the sealing film 32 is not left. See fig. 4-6. Methods of sealing attachment involved therein include, but are not limited to: sticking, etc. Specifically, as shown in fig. 4, a whole first adhesive layer 34 is disposed between the sealing film 32 and the elastic sealing layer 33; as shown in fig. 5, a whole second adhesive layer 34 'is disposed between the sealing film 32 and the elastic sealing layer 33, wherein the second adhesive layer 34' is open at a position corresponding to the hole 311, and the opening may be a rectangular opening or the like; as shown in fig. 6, a whole third adhesive layer 34 ″ is disposed between the sealing film 32 and the elastic sealing layer 33, wherein the third adhesive layer 34 ″ is opened at a position corresponding to the center of the hole 311, and the opening may be a circular opening or the like.

The elastic sealing layer 33 is provided as at least one sheet, wherein each sheet of the elastic sealing layer 33 seals at least one hole slot 311 of the sealing orifice plate body 31. Specifically, the elastic sealing layer 33 covers the top of the sealing orifice plate body 31, and may be a single elastic sealing layer covering and sealing all the orifice grooves 311 of the sealing orifice plate body 31, or may be a plurality of elastic sealing layers each covering and sealing at least one orifice groove 311 of the sealing orifice plate body 31. For example, for a 96-well plate, when there are two elastomeric sealing layers, each elastomeric sealing layer seals 48 well channels; when the number of the elastic sealing layers is 96, each elastic sealing layer seals 1 hole groove.

The sealed well plate 30, when empty, can be used as a sealed well plate for nucleic acid extraction processes used in nucleic acid extraction steps such as lysis, magnetic bead capture, washing, elution, etc.; it can also be used as a waste liquid sealing well plate for receiving waste liquid from a nucleic acid extraction process.

The present embodiment also proposes a reagent sealing well plate in which the same reagent is stored in all the well grooves 311 of the sealing well plate 30. See fig. 1-3. The technical solution of the sealing orifice plate 30 is described above, and is not described herein again.

The reagent sealing pore plate can be sealed and stored with reagents required by the sample nucleic acid extraction (such as lysis, magnetic bead capture, washing and elution) processes, such as: lysis solution, magnetic bead solution, washing solution or eluent become the nucleic acid extraction reagent sealing pore plate.

The reagent sealing pore plate can also be hermetically stored with reagents required by PCR amplification and real-time fluorescence detection, such as: and the PCR amplification reagent or the freeze-drying reagent becomes the PCR amplification reagent sealing pore plate.

This embodiment also proposes a pipetting system, comprising: the reagent sealing pore plate and/or the sealing pore plate are/is used for collecting a sampling pipe of a sample, a sampling pipe frame matched with the sampling pipe frame and used, and a pipette needle tube array. The sampling pipe frame is provided with at least one row and at least one column of sampling pipe grooves which are arranged in an array. The pipette needle tube array comprises at least one row of pipette needle tubes and at least one column of pipette needle tubes which are arranged in an array.

The distance between the central axes of the hole grooves in the sealing orifice plate 30, the distance between the central axes of the hole grooves in the reagent sealing orifice plate, the distance between the central axes of the sampling tube grooves and the distance between the central axes of the pipette needle tubes are the same. The sampling tube slot and the rows and the columns of the liquid transfer needle tubes are arranged identically, the number of the rows is N, and the number of the columns is M. The number of rows of the hole slots 311 is a × N, the number of columns is B × M, and N, M, a, and B are integers greater than 0. For example: when the sampling tube slot is 48 holes (M =6, n = 8); the wells 311 are 96 wells (M =12, n = 8), the array of wells 311 is 2 times the array of sample wells, and the array of syringes samples from the sample wells and loads the samples into the reagent sealing well plate and/or 2 sets of wells in the sealing well plate, respectively, and the 2 sets of wells can be used for 2-well detection of the same sample. When a and B are integers greater than 1, the array of the hole slots 311 in the sealing hole plate 30 is multiple of the array of the sampling tubes, and the method can be used for nucleic acid extraction and detection of the same sample, the same detection time and different targets.

The sealed orifice plate 30, the reagent sealed orifice plate, the pipette needle tube array, the sampling tube and the sampling tube rack can be used in an automatic PCR detector in a matching way.

Specifically, sampling pipe prepackage virus conserver liquid, after the sampling is accomplished, put into the sampling pipe with the sampling swab rupture, rotatory tube cap seals the chamber that holds of sampling pipe. The sampling tubes are placed in the sampling tube rack, and the array arrangement mode and the central shaft spacing of the sampling tubes are completely the same as those of the pipette needle tubes.

The sampling tube rack filled with the sampling tubes is placed in an automatic PCR (polymerase chain reaction) pipetting system and fixed, a mechanical arm in the PCR pipetting system can conveniently grab a pipetting needle tube array, each needle tube can conveniently align to the sampling tube and puncture a sealing gasket on a tube cover, and liquid is taken out in a containing cavity. When the liquid transfer needle tube is withdrawn, the self-sealing performance of the sealing gasket can automatically seal each sampling tube, so that a single sampling tube is always in a sealing state and cannot be polluted mutually. Because the surface area of the pipetting needle tube is far smaller than that of a common pipetting tube, the solution inevitably remained on the outer wall of the pipetting needle tube in the pipetting process is necessarily far smaller than that remained on the outer wall of the pipetting tube; when the pipette tip is withdrawn from the elastomeric seal 33, most of the solution is trapped inside the elastomeric seal 33 due to the squeezing of the elastomeric seal 33 against the tip, further reducing the amount of solution remaining on the outer wall of the pipette tip. Compared with the common pipetting tube with the outer wall space of about 4mm, the distance between the adjacent pipetting needle tubes is almost equal to 9mm, and the possibility of mutual pollution is further reduced.

Subsequently, when extracting the sample nucleic acid, the mechanical arm drives the pipette needle array to align with the sealed orifice plate/waste liquid sealed orifice plate/corresponding nucleic acid extraction reagent sealed orifice plate in the nucleic acid extraction process, the elastic sealing layer 33 and the sealing film 32 of the sealed orifice plate are punctured, liquid is discharged and taken from the orifice slot 311, and after the steps of cracking, magnetic bead adsorption, washing, elution and the like are completed, the template for completing the nucleic acid extraction is transferred to the PCR amplification reagent sealed orifice plate in which the PCR amplification reagent is stored through the pipette needle. The pipette tip array is aligned with the sealed orifice plate of the PCR amplification reagent, the elastic sealing layer 33 and the sealing film 32 are punctured, liquid is discharged in the orifice groove 311, and the sealed orifice plate of the PCR amplification reagent is used for PCR amplification and real-time fluorescence detection. Because the elastic sealing layer 33 is arranged on the upper part of the sealing orifice plate, the self-sealing effect can be realized, and the mutual pollution can be avoided.

Because the central axial distance of the sampling tube grooves of the sampling tube rack, the central axial distance of the hole grooves 311 of the sealing orifice plate and the reagent sealing orifice plate and the central axial distance of the pipette needle tube are the same, and the line and the arrangement are also the same or are in a multiple relation, the pipette needle tube does not need to carry out capping and cup separation operations before the sample nucleic acid is extracted, and the pipette needle tube does not need to carry out variable pitch, thereby not only saving a large amount of time for the extraction process of the sample nucleic acid, but also saving a large amount of equipment cost and time cost.

The above embodiments are merely to illustrate the technical solutions of the present application and are not limitative, and the present application is described in detail with reference to preferred embodiments. It will be understood by those skilled in the art that various modifications and equivalent arrangements may be made in the present invention without departing from the spirit and scope of the present invention and shall be covered by the appended claims.

Claims

1. A sealing orifice plate, comprising: the sealing pore plate comprises a sealing pore plate body, a sealing film and an elastic sealing layer, wherein at least one row and at least one column of pore grooves arranged in an array are arranged on the sealing pore plate body; the sealing membrane is in sealing connection with the top surface of the sealing pore plate body, so that each pore groove is sealed independently and isolated from each other; the elastic sealing layer is arranged on the sealing film in a sealing mode, each hole groove is sealed independently and isolated from each other through the elastic sealing layer, and the elastic sealing layer is made of elastic materials.

2. The sealing orifice plate of claim 1, wherein the center-to-center axial spacing of adjacent said orifice slots is 9mm.

3. The sealed aperture plate of claim 2, wherein the resilient sealing layer is in everywhere sealed connection with the bottom adjacent material.

4. The sealed aperture plate of claim 2, wherein the resilient sealing layer is sealingly connected to the bottom adjacent material above the top surface of the sealed aperture plate body and is not sealed above the aperture groove.

5. The sealed orifice plate of claim 2 in which the elastomeric sealing layer is not sealed over the central portion of the orifice well and is sealingly connected to the bottom adjacent material at all other locations.

6. The sealing aperture plate of claim 1, wherein the sealing membrane is provided in at least one sheet, wherein each sealing membrane seals at least one of the aperture slots.

7. The sealed aperture plate of claim 1, wherein said resilient sealing layer is provided as at least one sheet, wherein each said resilient sealing layer seals at least one said aperture slot.

8. The sealed aperture plate of claim 6, wherein the sealing membrane is sealingly attached to the top surface of the sealed aperture plate body by thermal molding, adhesive bonding, or ultrasonic welding.

9. The sealed aperture plate of claim 7, wherein the resilient sealing layer is bonded to the bottom material to effect a sealed connection.

10. A reagent sealed well plate based on a sealed well plate according to any of claims 1 to 9, wherein the same reagent is sealingly stored in all wells of the sealed well plate.

11. The reagent sealed well plate of claim 10, wherein the reagent comprises: lysis solution, magnetic bead solution, washing solution or eluent.

12. The reagent sealed well plate of claim 10, wherein the reagent comprises: PCR amplification reagents or lyophilized reagents.

13. A pipetting system, comprising: a sealed well plate according to any one of claims 1 to 9 and/or a reagent sealed well plate according to any one of claims 10 to 12, a sample tube for collecting a sample and a sample tube rack for use therewith, and an array of pipette tips; the sampling pipe frame is provided with at least one row of sampling pipe grooves arranged in an array manner and at least one column of sampling pipe grooves arranged in an array manner; the pipette needle tube array comprises at least one row of pipette needle tubes arranged in an array manner and at least one column of pipette needle tubes arranged in an array manner; the distance between the central axes of the hole grooves in the sealing hole plate, the distance between the central axes of the hole grooves in the reagent sealing hole plate, the distance between the central axes of the sampling tube grooves and the distance between the central axes of the pipette needle tubes are the same; the sampling tube groove the line number of transfer pipette tube is N, and the line number is M, sealed orifice plate the line number of hole groove is A N, and the line number is B M in the sealed orifice plate of reagent, wherein, N, M, A, B are the integer that is greater than 0, transfer pipette tube array be used for to get liquid in the sampling tube, to sealed orifice plate get the tapping liquid in the sealed orifice plate of reagent.