CN105733935A - Preparation and detection reagent card case for nucleic acid or protein - Google Patents
Preparation and detection reagent card case for nucleic acid or protein Download PDFInfo
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- CN105733935A CN105733935A CN201410767165.1A CN201410767165A CN105733935A CN 105733935 A CN105733935 A CN 105733935A CN 201410767165 A CN201410767165 A CN 201410767165A CN 105733935 A CN105733935 A CN 105733935A
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Abstract
A preparation and detection reagent card case for nucleic acid or protein includes a case body provided with an open-close-type object placing opening and an open-close-type object taking opening. An extraction reaction chamber, an extraction reagent storage chamber, a cleaning chamber, an elution chamber and a detection reaction chamber are formed in the case body. The cleaning chamber is arranged between the extraction reaction chamber and the elution chamber, and solid transfer mechanisms are arranged on two ends of the cleaning chamber and are respectively connected to the extraction reaction chamber and the elution chamber adjacently. The solid transfer mechanisms serve as separation bodies for adjacent chambers when being idle and form paths between the adjacent chambers when being occupied to provide oriented magnetic force for transfer of the solid material. Through the solid transfer mechanisms having an individual structure, magnetic solid materials are transferred step-by-step among the chambers, so that the whole structure of the card case is greatly simplified. The card case integrates preparation and detection of the nucleic acid or protein, overcomes the problems that the extraction, detection and connecting steps therebetween have complex operations and samples or reagents are liable to be polluted.
Description
Technical field
The present invention relates to the consumptive material that the preparation of nucleic acid or protein is used with detection, particularly relate to a kind of nucleic acid or albumen preparation and detectable cartridge.
Background technology
In the past, the detection Main Basis medical history of disease, symptom, sign and various auxiliary examination, such as biochemistry, hematology, pathology, immunology, microbiology etc..But owing to above-mentioned inspection method all has respective limitation so that numerous disease fails to be detected timely and accurately and delay treatment good opportunity.
Nowadays, along with the development of the transformation of medical model and personalized medicine, medical test circle is in the urgent need to detection means fast, accurately, and Molecular Detection has then given play to the advantage of uniqueness.
The basis of Molecular Detection is to analyze the histiocyte of detected person, hair, anticoagulation or blood stasis mark, and formaldehyde is fixed, gene in paraffin-embedded tissue and expression product thereof, by completing nucleic acid (DNA and RNA) detection from molecular level, in disease once generation even not yet occurs before symptom, sign and biochemical change, detection just can accurately be made.
At present, molecular detection technology mainly has making nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip technology etc..Molecular Detection product is mainly used in the aspects such as the detection of the clinical departments such as tumor, infection, heredity, Prenatal Screening, and MEC, technical service center, third party testing agency and microbial rapid detection market.Currently, the measuring means such as blood routine, cytology, pathology and immunology all develop towards automatization, integration, standardization direction, but Molecular Detection is due to himself technical complexity, the full-automatic instrument platform of " from sample to result " is few or there is the problems such as such as cost height, poor sensitivity, structure complexity, operation and associated assay devices excessively complexity.
Summary of the invention
For deficiency of the prior art, present invention solves the technical problem that and be in that to provide a kind of nucleic acid or albumen preparation and detectable cartridge, integrate the preparation of nucleic acid or albumen and detection, overcome extractions, detection and the two be mutually linked in step the problem of the trivial operations of existence, sample or reagent vulnerable to pollution.
In order to solve above-mentioned technical problem, offer one nucleic acid or albumen that the embodiment of the present invention provides are prepared and detectable cartridge, and including the box body formed by outer wall, described box body has open-close type placing port and takes thing mouth, it is characterised in that:
In described box body, it is formed with extraction reaction warehouse, extraction reagent storage warehouse, cleans storehouse, eluting storehouse and detect reaction warehouse；
Wherein, described extraction reaction warehouse is connected with the external world by placing port, described in take thing mouth and be arranged at described detection reaction warehouse or eluting storehouse and be connected with the external world；
Described extraction reagent storage warehouse is adjacent to extraction reaction warehouse, being provided with the isolation face that can puncture between described extraction reaction warehouse and extraction reagent storage warehouse in described extraction reagent storage warehouse so that the two through and to extraction reaction warehouse injection extraction reagent injecting mechanism, at least one of described extraction reagent storage warehouse, storage has the extraction reagent containing magnetic-particle；
Described cleaning position in storehouse is between described extraction reaction warehouse and eluting storehouse, its two ends have the solid transfer device being adjacent to extraction reaction warehouse respectively with eluting storehouse, described solid transfer device as the slider between adjacent storehouse, and operationally can form the path between adjacent storehouse and the transfer for solid material provides directional magnetic force when not working；
Connected by conduit between described detection reaction warehouse and eluting storehouse and liquid material after obtaining eluting carries out detection reaction.
As the preferred version of the present invention, a kind of nucleic acid of embodiments of the invention offer or albumen preparation and detectable cartridge farther include the part or all of of techniques below feature:
Preferably, described solid transfer device includes two switching mechanisms be arrangeding in parallel, described switching mechanism includes two interval bodies being disposed adjacent and mutually suppressing and make solid transfer device close, the magnetic field of the electromagnet formation adsorbent solid material that solid transfer device both sides are arranged it is positioned at outside box body, when external force longitudinally extrudes described interval body, producing top clearance between the interval body of two Guan Bis, electromagnet produces directional magnetic field, and the transfer for solid material provides directional magnetic force.
Preferably, solid material is being extracted reaction warehouse by this solid transfer device and is being cleaned between storehouse, adjacent cleaning storehouse, cleans and shift step by step between storehouse and eluting storehouse.
Preferably, described cleaning storehouse is set to one or more；When being set to two or more, also solid transfer device between adjacent cleaning storehouse.
Preferably, described detection reaction warehouse is provided with the charge door adding detectable from the external world, or has been provided adjacent to detectable storage silo.
Preferably, the some or all of employing light-passing plastic of described box body is prepared from, and the light-permeable part of described box body comprises this region residing for detection reaction warehouse.
Preferably, described cleaning storehouse and eluting storehouse are provided above liquid storage silo, being provided with in described liquid storage silo and can puncture described liquid storage silo and clean the isolation face in storehouse, isolation face between liquid storage silo and eluting storehouse so that the two is through and can increase the ejector of the mobility of fluid in storehouse, the top of described ejector arranges fluid mobility in promising increase storehouse and provides the handle of disturbance repeatedly.
Preferably, the bottom of described extraction reaction warehouse, eluting storehouse and detection reaction warehouse is provided with the also contacts point for heating；The bottom in the anti-storehouse of described extraction is additionally provided with ultrasound wave contact point, and this ultrasound wave contact point and the also contacts point extracting reaction warehouse separate or be wholely set.
Preferably, described also contacts point is prepared from by copper, ceramic or optimum Heat Conduction Material.
Preferably, described extraction reagent storage warehouse is provided with one or more, and at least one of extraction reagent storage warehouse, storage has the extraction reagent containing magnetic-particle, and this magnetic-particle can be adsorbed on extraction reaction warehouse absorption nucleic acid or protein.
Nucleic acid or albumen that the embodiment of the present invention provides are prepared and detectable cartridge, have the advantages that
The nucleic acid of embodiment of the present invention offer or albumen preparation and detectable cartridge by being formed with the extraction reaction warehouse that material is through in box body, extract reagent storage warehouse, clean storehouse, eluting storehouse and detection reaction warehouse, make such as nucleic acid or albumen can realize preparation and detection in a confined space, need under open environment and by manually step-by-step being operated relative in prior art, cartridge provided by the present invention is facility simple to operate not only, integrate preparation and the detection of nucleic acid or albumen, overcome extraction, detection and the two be mutually linked in step exist trivial operations, the problem of sample or reagent vulnerable to pollution；And, all of extraction and detecting step all can carry out in same confined space, also reduce the probability of sample contamination, improve the purity of extract and the accuracy of detection.Additionally, the nucleic acid of the present invention or albumen are prepared and in detectable cartridge, by arranging the solid transfer device that structure is unique, make solid transfer device when not working as the slider between adjacent storehouse, and operationally, solid transfer device can be made to form the path between adjacent storehouse by the solid transfer device in external force longitudinal direction extrusion solid transfer device and the transfer for solid material provides directional magnetic force, realize magnetic retention material extracting reaction warehouse and cleaning storehouse, between adjacent cleaning storehouse, clean and shift step by step between storehouse and eluting storehouse, greatly simplifie nucleic acid of the present invention or the overall structure of albumen preparation and detectable cartridge.
Accompanying drawing explanation
Fig. 1 is the nucleic acid that provides of the preferred embodiment of the present invention or albumen preparation and the structural representation of detectable cartridge.
Fig. 2 is the solid transfer device structural representation in a quiescent state in the nucleic acid that provides of the preferred embodiment of the present invention or albumen preparation and detectable cartridge.
Fig. 3 be the preferred embodiment of the present invention provide nucleic acid or albumen preparation and detectable cartridge in solid transfer device in working order under structural representation.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art obtain under not making creative work premise, broadly fall into the scope of protection of the invention.
Figure is the nucleic acid that provides of the preferred embodiment of the present invention or albumen preparation and the structural representation of detectable cartridge.This nucleic acid or albumen preparation and detectable cartridge are applied in nucleic acid or albumen preparation and detection equipment, can be used in carrying out extracting to the such as object such as nucleic acid or albumen and detect, but being not limited thereto, in actual applications, described extraction box also apply be applicable to the extraction of other biological extract.
As shown in Figure 1, the nucleic acid of preferred embodiment of the present invention offer or albumen preparation and detectable cartridge include the box body 1 formed by outer wall, in box body, it is formed with extraction reaction warehouse 6, extracts reagent storage warehouse 9,10,11 and 12, clean storehouse 20 and 23, eluting storehouse 41 and detection reaction warehouse 26.Box body 1 has open-close type placing port 2 and takes thing mouth 3, and when implementing, placing port 2 is arranged at extraction reaction warehouse 6, takes thing mouth 3 and is arranged at detection reaction warehouse 26.The needs that arrange for taking thing mouth 3 consider to complete the purposes of the product of extraction step behind eluting storehouse, if completing test experience either directly through detection reaction warehouse 26, then in the present embodiment, scheme used will take thing mouth 3 and be arranged at detection reaction warehouse 26；If needing the product completing extraction step behind eluting storehouse is used for other detection test or is used as his way, then it is arranged at eluting storehouse 41 can obtain superior in quality nucleic acid, protein or other biological extract by taking thing mouth 3.It addition, in embodiments of the present invention, the overall body structure in upper shed of box body 1, and can be made by such as plastic material.Placing port 2 is the end face being opened in box body 1 with taking thing mouth 3, and its shape can be circular hole, but is not limited thereto, it is also possible to be square hole, polygonal hole or even erose perforate.And be further provided with on the end face of box body 1 with placing port 2 with take the thread sealed closure that thing mouth 3 coordinates.Certainly, in a further embodiment, to placing port 2 with take the structure that thing mouth 3 seals be also configured to its perisporium additional have the packing ring or strip of paper used for sealing that can reach obturation effect, no longer repeat separately at this.
Extract reaction warehouse 6 be positioned at box body 1 and be connected with placing port 2.In the present embodiment, extracting reaction warehouse 6 other bins relative and have bigger accommodation space, it is one as sample extraction important reaction bin, for being obtained by reacting intermediate product after mixing with reagent for sample.
Extract reagent storage warehouse and be adjacent to extraction reaction warehouse 6.It practice, the kind according to reagent required in the characteristic of extract and the reactions steps of correspondence, the quantity extracting reagent storage warehouse both can be one, it is also possible to is multiple.When the quantity of reagent storage warehouse is multiple, each reagent storage warehouse both can be store same reagent, it is also possible to is store reagent not of the same race respectively, and, each reagent storage warehouse can have different sizes and shape according to the dosage of built-in reagent.In embodiments of the present invention, extract reagent storage warehouse to include four and extract reagent storage warehouses 9,10,11 and 12, and extract reagent storage warehouse 9,10,11 and 12 be by such as thin film, to mould the isolation face 15 that the slim material such as paper makes isolated with the extraction reaction warehouse 6 in phase portion.
For extracting reaction warehouse 6 and the Flow of Goods and Materials extracted between reagent storage warehouse, before not carrying out extraction reaction, extraction reagent storage warehouse 9,10,11 and 12 is isolated and logical mutually with extracting reaction warehouse 6；And when needs react, it is necessary to get through and extract reagent storage warehouse 9,10,11 and 12 for isolating and extract the isolation face 15 of reaction warehouse 6, so that extracting reagent stored in reagent storage warehouse 9,10,11 and 12 can enter extraction reaction warehouse 6 and sample mix.Therefore, it is provided with in carrying and can puncture the isolation face 15 extracted between reaction warehouse 6 and extraction reagent storage warehouse 9,10,11 and 12 so that reaction warehouse is through with extraction reagent storage warehouse and injects, to extracting reaction warehouse 6, the injecting mechanism 91,101,111 and 121 extracting reagent.In the present embodiment, injecting mechanism 91,101,111 and 121 is be right against the skilful pricker in isolation face or rifle head, and described pricker or the force-applied start of rifle head and then the isolation face of piercing through 15 are got through extraction reagent storage warehouse 9,10,11 and 12 and extract reaction warehouse 6.It is true that in other embodiments that the present invention is feasible, as long as can realize reagent storage warehouse ingeniously and extract the through of reaction warehouse 6 in damage containment face, described puncturing mechanism may be designed in other mechanisms such as such as drill bit.And injecting mechanism not only includes the skilful pricker in the isolation face that is right against or rifle head, also including piston-like movement structure, this piston-like movement structure can enable to be placed in the extraction reagent extracting reagent storage warehouse substantially effectively from extracting reagent storage warehouse injection extraction reaction warehouse 6.
In order to ensure the abundant absorption of extraction process, extract in reaction warehouse 6 and be additionally provided with perturbation mechanism 16.In the present embodiment, perturbation mechanism 16 is in extracting the bottom of reaction warehouse 6, and it is specially the air pump of squash type, can be increased by the running of described air pump and extract sample and the mobility of reagent in reaction warehouse 6, increases the uniformity of the two mixing and accelerate response speed.Because of the prior art that gas pump structure and operation principle thereof are well known to those skilled in the art, therefore do not repeat them here, as long as it practice, the mobility of storehouse fluid inside can be increased, described perturbation mechanism can also be such as mechanical type or DYN dynamic agitator, should have similar effect.
It should be noted that, owing to the nucleic acid of the present invention or albumen preparation and detectable cartridge are applicable for use with the magnetic-particle including magnetic bead as extracting nucleic acid, albumen or other macromole bio-extracts, therefore, in the various implementations of the present invention, extracting storage at least one in reagent storage warehouse 9,10,11 and 12 has the extraction reagent containing magnetic-particle.Certainly in a preferred embodiment of the invention, which the reagent that extracts containing magnetic-particle is positioned over and extracts reagent storage warehouse and can take into full account in interior factor according to extracting the quantity of reagent storage warehouse, position and volume.
Clean position in storehouse in extracting between reaction warehouse 6 and eluting storehouse 41.The effect in described cleaning storehouse is to be in that the intermediate product that the mixed liquor of sample in extracting reaction warehouse 6 with reagent is obtained by reacting is carried out so that it is removes impurity and obtains the intermediate product of compliant.The two ends cleaning storehouse have the solid transfer device being adjacent to extraction reaction warehouse 6 respectively with eluting storehouse 41, solid transfer device as the slider between adjacent storehouse, and operationally can form the path between adjacent storehouse and the transfer for solid material provides directional magnetic force when not working.
When implementing, in the embodiment of the present invention, extract reaction warehouse 6 and eluting storehouse 41 is provided with two cleaning storehouses 20,23.Certainly in other embodiments of the invention, clean storehouse also can be set to according to specific needs one or and two or more, when being set to two or more, also solid transfer device between adjacent cleaning storehouse.
Corresponding, in embodiments of the present invention, it is provided with three solid transfer devices 19,22 and 25 altogether, wherein, solid transfer device 19 is arranged at extraction reaction warehouse 6 and cleans between storehouse 20, solid transfer device 22 is arranged at two and cleans between storehouse 20,23, and solid transfer device 25 is arranged between cleaning storehouse 23 and eluting storehouse 41.In fact, the kind of required reagent in characteristic according to intermediate product and the reactions steps of correspondence, the quantity cleaning storehouse can be one or more, the quantity of solid transfer device can be two or more than two multiple, in actual applications, solid transfer device is in cleaning relative two ends in storehouse, therefore, in general, the quantity of solid transfer device is than many one of the quantity cleaning storehouse.Being referred to described above accordingly, with respect to solid transfer device be adjusted with the set-up mode and number cleaning storehouse, therefore not to repeat here.
Structure about solid transfer device, for solid transfer device 19, it includes two switching mechanisms be arrangeding in parallel 34,35 (with it similarly, solid transfer device 22 includes two switching mechanisms be arrangeding in parallel 36,37, and solid transfer device 25 includes two switching mechanisms be arrangeding in parallel 38,39).As in figure 2 it is shown, switching mechanism 34 includes two interval bodies 341,342 being disposed adjacent and mutually suppressing and make solid transfer device close, outside box body, it is positioned at the magnetic field of electromagnet 191, the 192 formation adsorbent solid material that solid transfer device both sides are arranged.When external force longitudinally extruding interval body 341,342, as shown in Figure 3, producing top clearance 343 between the interval body 341,342 of two Guan Bis, electromagnet 191,192 all produces to surround the annular orientation magnetic field of electromagnet, and the transfer for solid material provides directional magnetic force.In principle, the present invention is mainly by adopting the magnetic-particle including magnetic bead as extracting nucleic acid, albumen or other macromole bio-extracts, after extracting reaction warehouse 6 and completing to extract reaction, the magnetic-particle extracted in reaction warehouse 6 in mixed material is adsorbed with nucleic acid to be extracted, albumen or other macromole bio-extracts.Now, the magnetic-particle extracted in reaction warehouse 6 in mixed material can be effectively transferred to clean in storehouse 20 by magnetic force by the effect of solid transfer device 19, and ratio is avoided being mixed into too much impurity.nullIn this transfer process，External force first longitudinally extrudes the interval body 341 of switching mechanism 34、342，The interval body 341 of two Guan Bis、Top clearance 343 is produced between 342，Electromagnet 191、192 produce directional magnetic field makes to be adsorbed with nucleic acid to be extracted、The magnetic-particle of albumen or other macromole bio-extracts is transferred in solid transfer device 19，After then switching mechanism 34 external force to be extruded disappears，Apply longitudinally to extrude external force to switching mechanism 35 again，Make generation top clearance between two interval bodies of switching mechanism 35，Now，Make to be adsorbed with nucleic acid to be extracted by cleaning the flow disturbance in storehouse 20、The magnetic-particle of albumen or other macromole bio-extracts is transferred to cleaning storehouse 20，Then extruding switching mechanism 35 external force disappears，Two interval bodies of switching mechanism 35 mutually suppress split and form sealing structure，Namely the solid material transfer extracted reaction warehouse 6 and clean between storehouse 20 is completed.With it similarly, two are cleaned between storehouses 20,23, and cleaning the transfer of the solid material between storehouse 23 and eluting storehouse 41 is also that the identical operation of employing completes.For whole extraction process, including the extraction extracted in reaction warehouse 6 and adsorption reaction, clean and remove deimpurity purification reaction in storehouse 20,23, solid material is being extracted reaction warehouse by this solid transfer device and is being cleaned between storehouse, adjacent cleaning storehouse, cleans and shift step by step between storehouse and eluting storehouse, rather than namely a step is entered eluting storehouse 41 either directly through cleaning storehouse 20,23 by extracting reaction warehouse 6.Namely it is adsorbed with the magnetic-particle transfer of nucleic acid to be extracted, albumen or other macromole bio-extracts first to transfer to via solid transfer device 19 and clean storehouse 20 and carry out the cleaning of the first step and remove impurity by extracting reaction warehouse 6, then transferred to cleaning storehouse 23 by cleaning storehouse 20 via solid transfer device 22 again and carry out the cleaning removal impurity of second step, then transferred to eluting storehouse 41 eluting acquisition extract by cleaning storehouse 23 via solid transfer device 25.
In the invention process, cleaning storehouse 20,23 is isolated, by the flap such as extended to form from box body 1 diapire, the confined space formed.Clean storehouse 20,23 and eluting storehouse 41 and be provided above liquid storage silo 21,24,27, be provided with in liquid storage silo 21,24,27 and can puncture liquid storage silo 21,24 and clean the isolation face in storehouse 20,23, isolation face between liquid storage silo 27 and eluting storehouse 41 so that the two is through and can increase the ejector 31,32 and 33 of the mobility of fluid in storehouse.In order to strengthen disturbance effect and operability, the top of ejector 31,32 and 33 arranges fluid mobility in promising increase storehouse and provides the handle of disturbance repeatedly.When implementing, clean storehouse 20,23 equipped with cleaning reagent, so that the impurity gone out in solid material, by the effect of ejector 32,33 can make cleaning reagent with clean storehouse 20,23 and be adsorbed with nucleic acid to be extracted, the magnetic-particle of albumen or other macromole bio-extracts is fully contacted thus the impurity effectively gone out in extract.Certainly, cleaning reagent can also leave in liquid storage silo 21,24, injecting either directly through ejector 32,33 in cleaning process cleans in storehouse 20,23, certainly in other embodiments of the invention, as needed in cleaning storehouse 20,23 and the cleanout fluid depositing different usefulness in liquid storage silo 21,24 respectively, and liquid storage silo 21,24 can also be determined whether as required be injected in cleaning storehouse 20,23.With it similarly, eluting storehouse 41 is equipped with eluent, to dissolve nucleic acid, albumen or other macromole bio-extracts, the magnetic-particle being adsorbed with nucleic acid, albumen or other macromole bio-extracts in eluent and eluting storehouse 41 can be made to be fully contacted thus effectively dissolving extract by the effect of ejector 31.Certainly, eluent can also leave in liquid storage silo 27, elution process is injected in eluting storehouse 41 either directly through ejector 27, certainly in other embodiments of the invention, as needed in eluting storehouse 41 and the eluent depositing different usefulness in liquid storage silo 27 respectively, and liquid storage silo 27 can also be determined whether as required be injected in eluting storehouse 41.
In the invention process, for detection reaction warehouse 26, connected by conduit 40 between its with eluting storehouse 41, increase the air pressure in eluting storehouse 41 by ejector 31 to injection gas in eluting storehouse 41, eluting storehouse 41 liquid material can be made to flow into detection reaction warehouse 26 by conduit 40.Certainly adopt other mode of communicating that the liquid material in eluting storehouse 41 can be made to flow into detection reaction warehouse 26 also when being alternative.When implementing, detection reaction warehouse 26 is provided with detectable storage silo.In the solution of the present invention, detection reaction warehouse 26 two detectable storage silo 29,30 are provided adjacent to.Can refer to, about the number of detectable storage silo, set-up mode and detectable storage silo and the position relationship and material circulation style that detect reaction warehouse, the set-up mode extracting reagent storage warehouse and extraction reaction warehouse to be determined.It should be noted that, in other embodiments of the invention, it is to detect to determine its index such as composition and physicochemical property to the extract obtained owing to arranging detection reaction warehouse, and the purpose detected and mode are also very various, therefore, the charge door adding detectable from the external world can also be provided with according to specific needs on detection reaction warehouse, and backward detection reaction warehouse 26 adds the detectable based on different purposes and detects.
It addition, for the technical purpose realizing extraction, eluting and detection, be provided with the also contacts point 17,28 and 42 for heating in the bottom extracting reaction warehouse, eluting storehouse and detection reaction warehouse.Due to arrange also contacts point in order that realize temperature control better, therefore, the optimum Heat Conduction Material of also contacts point is prepared from, in the invention process, also contacts point adopt high-heat-conductivity ceramic material be prepared from.The optimum heat-conducting metals such as certain copper, silver are also good candidate materials.And, in embodiments of the present invention, the bottom extracting anti-storehouse is additionally provided with ultrasound wave contact point, cell, tissue in extraction process are waited that extracting material crushes effectively, macromole nucleic acid molecules can also be made simultaneously to fragment into small molecular core acid to obtain goal object, and strengthen absorption, extraction effect.This ultrasound wave contact point and the also contacts point extracting reaction warehouse are wholely set, and namely in the present embodiment, also contacts point 17 is also ultrasound wave contact point simultaneously.Certainly, in other embodiments of the invention, it is also possible to be provided separately to reach corresponding technical purpose with also contacts point 17 by ultrasound wave contact point.
Being provided with perturbation mechanism 16 it addition, extract bottom reaction warehouse 6, this perturbation mechanism 16 is the air pump of squash type, can be increased by the running of air pump and extract sample and the mobility of reagent in reaction warehouse 6, increases the uniformity of the two mixing and accelerate response speed.Because of the prior art that gas pump structure and operation principle thereof are well known to those skilled in the art, therefore do not repeat them here, as long as it practice, the mobility of storehouse fluid inside can be increased, described perturbation mechanism can also be such as mechanical type or DYN dynamic agitator, should have similar effect.Moreover, solid transfer valve 18 is also included in the bottom extracting reaction warehouse 6, solid transfer valve 18 connects with extracting reaction warehouse 6.In the present embodiment, it is a less accommodation space that solid transfer valve 18 extracts reaction warehouse 6 relatively, and now, perturbation mechanism 16 can be directly arranged in solid transfer valve 18.So, mainly carry out in solid transfer valve 18 exactly from the reaction of the mixed liquor of actual effect, sample and reagent.The setting of solid transfer valve 18 can make the reaction of the mixed liquor of sample and reagent be able to carry out in the bin that space is suitable so that reacts more abundant.
The nucleic acid of above-described embodiment announcement or albumen preparation and detectable cartridge by being formed with the extraction reaction warehouse that material is through in box body, extract reagent storage warehouse, clean storehouse, eluting storehouse and detection reaction warehouse, make such as nucleic acid or albumen can realize preparation and detection in a confined space, need under open environment and by manually step-by-step being operated relative in prior art, cartridge provided by the present invention is facility simple to operate not only, integrate preparation and the detection of nucleic acid or albumen, overcome extraction, detection and the two be mutually linked in step exist trivial operations, the problem of sample or reagent vulnerable to pollution；And, all of extraction and detecting step all can carry out in same confined space, also reduce the probability of sample contamination, improve the purity of extract and the accuracy of detection.Additionally, the nucleic acid of the present invention or albumen are prepared and in detectable cartridge, by arranging the solid transfer device that structure is unique, make solid transfer device when not working as the slider between adjacent storehouse, and operationally, solid transfer device can be made to form the path between adjacent storehouse by the solid transfer device in external force longitudinal direction extrusion solid transfer device and the transfer for solid material provides directional magnetic force, realize magnetic retention material extracting reaction warehouse and cleaning storehouse, between adjacent cleaning storehouse, clean and shift step by step between storehouse and eluting storehouse, greatly simplifie nucleic acid of the present invention or the overall structure of albumen preparation and detectable cartridge.
The above is the preferred embodiment of the present invention; it should be pointed out that, for those skilled in the art, under the premise without departing from the principles of the invention; can also making some improvements and modifications, these improvements and modifications are also considered as protection scope of the present invention.
Claims (10)
1. nucleic acid or albumen are prepared and a detectable cartridge, and including the box body formed by outer wall, described box body has open-close type placing port and takes thing mouth, it is characterised in that:
In described box body, it is formed with extraction reaction warehouse, extraction reagent storage warehouse, cleans storehouse, eluting storehouse and detect reaction warehouse；
Wherein, described extraction reaction warehouse is connected with the external world by placing port, described in take thing mouth and be arranged at described detection reaction warehouse or eluting storehouse and be connected with the external world；
Described extraction reagent storage warehouse is adjacent to extraction reaction warehouse, being provided with the isolation face that can puncture between described extraction reaction warehouse and extraction reagent storage warehouse in described extraction reagent storage warehouse so that the two through and to extraction reaction warehouse injection extraction reagent injecting mechanism, at least one of described extraction reagent storage warehouse, storage has the extraction reagent containing magnetic-particle；
Described cleaning position in storehouse is between described extraction reaction warehouse and eluting storehouse, its two ends have the solid transfer device being adjacent to extraction reaction warehouse respectively with eluting storehouse, described solid transfer device as the slider between adjacent storehouse, and operationally can form the path between adjacent storehouse and the transfer for solid material provides directional magnetic force when not working；
Connected by conduit between described detection reaction warehouse and eluting storehouse and liquid material after obtaining eluting carries out detection reaction.
2. nucleic acid as claimed in claim 1 or albumen are prepared and detectable cartridge, it is characterized in that: described solid transfer device includes two switching mechanisms be arrangeding in parallel, described switching mechanism includes two interval bodies being disposed adjacent and mutually suppressing and make solid transfer device close, the magnetic field of the electromagnet formation adsorbent solid material that solid transfer device both sides are arranged it is positioned at outside box body, when external force longitudinally extrudes described interval body, top clearance is produced between the interval body of two Guan Bis, electromagnet produces directional magnetic field, transfer for solid material provides directional magnetic force.
3. nucleic acid as claimed in claim 2 or albumen are prepared and detectable cartridge, it is characterised in that: solid material is being extracted reaction warehouse by this solid transfer device and is being cleaned between storehouse, adjacent cleaning storehouse, cleans and shift step by step between storehouse and eluting storehouse.
4. nucleic acid as claimed in claim 1 or albumen are prepared and detectable cartridge, it is characterised in that: described cleaning storehouse is set to one or more；When being set to two or more, also solid transfer device between adjacent cleaning storehouse.
5. nucleic acid as claimed in claim 1 or albumen are prepared and detectable cartridge, it is characterised in that: described detection reaction warehouse is provided with the charge door adding detectable from the external world, or has been provided adjacent to detectable storage silo.
6. nucleic acid as claimed in claim 1 or albumen are prepared and detectable cartridge, it is characterised in that: the some or all of employing light-passing plastic of described box body is prepared from, and the light-permeable part of described box body comprises this region residing for detection reaction warehouse.
7. nucleic acid as claimed in claim 1 or albumen are prepared and detectable cartridge, it is characterized in that: described cleaning storehouse and eluting storehouse are provided above liquid storage silo, being provided with in described liquid storage silo and can puncture described liquid storage silo and clean the isolation face in storehouse, isolation face between liquid storage silo and eluting storehouse so that the two is through and can increase the ejector of the mobility of fluid in storehouse, the top of described ejector arranges fluid mobility in promising increase storehouse and provides the handle of disturbance repeatedly.
8. nucleic acid as claimed in claim 1 or albumen are prepared and detectable cartridge, it is characterised in that: the bottom of described extraction reaction warehouse, eluting storehouse and detection reaction warehouse is provided with the also contacts point for heating；The bottom in the anti-storehouse of described extraction is additionally provided with ultrasound wave contact point, and this ultrasound wave contact point and the also contacts point extracting reaction warehouse separate or be wholely set.
9. nucleic acid as claimed in claim 1 or albumen are prepared and detectable cartridge, it is characterised in that: described also contacts point is prepared from by copper, ceramic or optimum Heat Conduction Material.
10. nucleic acid as claimed in claim 1 or albumen are prepared and detectable cartridge, it is characterized in that: described extraction reagent storage warehouse is provided with one or more, and storage has the extraction reagent containing magnetic-particle at least one of extraction reagent storage warehouse, this magnetic-particle can be adsorbed on extraction reaction warehouse absorption nucleic acid or protein.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410767165.1A CN105733935A (en) | 2014-12-12 | 2014-12-12 | Preparation and detection reagent card case for nucleic acid or protein |
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| CN201410767165.1A CN105733935A (en) | 2014-12-12 | 2014-12-12 | Preparation and detection reagent card case for nucleic acid or protein |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107561262A (en) * | 2017-09-30 | 2018-01-09 | 郑州安图生物工程股份有限公司 | Integral biological and chemical reaction equipment |
| CN107621550A (en) * | 2017-11-08 | 2018-01-23 | 苏州市苏测检测技术有限公司 | Food elution detection integration apparatus |
| CN109234141A (en) * | 2018-05-09 | 2019-01-18 | 奥然生物科技（上海）有限公司 | A kind of biological reaction apparatus being provided with micro-fluidic structure |
| CN109266518A (en) * | 2018-11-29 | 2019-01-25 | 奥然生物科技（上海）有限公司 | It is a kind of to be provided with micro-fluidic or nanometer flow control structure biological reaction apparatus |
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| WO2022143458A1 (en) * | 2020-12-29 | 2022-07-07 | 安图实验仪器(郑州)有限公司 | Cartridge preprocessing system for use in automated molecular diagnostics instrument |
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2014
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107561262A (en) * | 2017-09-30 | 2018-01-09 | 郑州安图生物工程股份有限公司 | Integral biological and chemical reaction equipment |
| CN107621550A (en) * | 2017-11-08 | 2018-01-23 | 苏州市苏测检测技术有限公司 | Food elution detection integration apparatus |
| CN109234141A (en) * | 2018-05-09 | 2019-01-18 | 奥然生物科技（上海）有限公司 | A kind of biological reaction apparatus being provided with micro-fluidic structure |
| CN109234141B (en) * | 2018-05-09 | 2022-01-25 | 奥然生物科技（上海）有限公司 | Be provided with micro-fluidic structure's biological reaction device |
| CN109266518A (en) * | 2018-11-29 | 2019-01-25 | 奥然生物科技（上海）有限公司 | It is a kind of to be provided with micro-fluidic or nanometer flow control structure biological reaction apparatus |
| CN110195017A (en) * | 2019-06-11 | 2019-09-03 | 郑州大学第二附属医院 | A kind of Parkinson's disease Disease-causing gene Mutation Screening detection method |
| CN110195017B (en) * | 2019-06-11 | 2022-04-15 | 郑州大学第二附属医院 | Parkinson disease pathogenic gene mutation screening and detecting method |
| WO2022143458A1 (en) * | 2020-12-29 | 2022-07-07 | 安图实验仪器(郑州)有限公司 | Cartridge preprocessing system for use in automated molecular diagnostics instrument |
| CN115561474A (en) * | 2022-12-08 | 2023-01-03 | 深圳市赛特罗生物医疗技术有限公司 | Integrated pesticide residue standardized rapid detection device and method |
| CN115561474B (en) * | 2022-12-08 | 2024-01-02 | 深圳市赛特罗生物医疗技术有限公司 | Integrated pesticide residue standardized rapid detection device and method |
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