CN214458016U - Extraction layer and amplification layer connecting structure of nucleic acid detection system - Google Patents
Extraction layer and amplification layer connecting structure of nucleic acid detection system Download PDFInfo
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- CN214458016U CN214458016U CN202022259445.5U CN202022259445U CN214458016U CN 214458016 U CN214458016 U CN 214458016U CN 202022259445 U CN202022259445 U CN 202022259445U CN 214458016 U CN214458016 U CN 214458016U
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Abstract
The utility model discloses a nucleic acid detecting system's extraction layer and amplification layer connection structure, including extraction layer, sealed rotation connect the waste liquid layer of extraction layer below, with waste liquid layer seal fixed connection's amplification layer and push the piston assembly of liquid, extract layer and waste liquid layer, be equipped with a plurality of liquid flow channel on extraction layer and the amplification layer and with the air flow channel that liquid flow channel cooperation was used, through rotating extraction layer selectively with extraction layer and the liquid flow channel and the air flow channel intercommunication on amplification layer. The utility model discloses a draw layer, sealed rotation connect in draw the waste liquid layer of layer below, with waste liquid layer sealed fixed connection's the augmentation layer and push the piston assembly of liquid, through rotating draw layer selectively will draw the layer and correspond the liquid flow channel and the air current channel intercommunication that augment the pipe, promote the piston assembly at middle part and make and draw the intraformational liquid flow in the corresponding augmentation pipe, the utility model discloses a part structure is few, arranges the compactness, and is small, the simple operation, and the preparation is convenient, reduces the cost of manufacture.
Description
Technical Field
The utility model relates to a nucleic acid testing equipment technical field, in particular to nucleic acid testing system's extraction layer and amplification layer connection structure.
Background
Laboratory tests are currently an important means for screening and diagnosing pathogenic bacteria or virus infections, and mainly comprise culture tests, microscopic tests, immunological tests and nucleic acid tests. The microscopic examination method is low in cost, simple and convenient, but has low positive rate and is easy to miss examination; the result reliability of the culture method is high, but the period is long and the cost is high; the immunological detection is simple to operate, but the detection is easy to miss due to a window period, and false positive results are often caused due to insufficient specificity of the antigen and the antibody.
The nucleic acid detection mainly takes PCR detection as a main part, the PCR is a method for synthesizing specific DNA fragments in vitro by enzymatic synthesis, and the method comprises a cycle consisting of high-temperature denaturation, low-temperature annealing and room-temperature extension reaction, so that the target DNA can be rapidly amplified. The PCR technology is very simple, has the advantages of high sensitivity, high specificity, short detection time and the like, has permeated into various biological branch science and clinical laboratory, and has important practical application value in many scientific fields of molecular biology, medical research, life science, bioengineering, genetic engineering, disease diagnosis, forensic medicine, archaeology and the like. Meanwhile, in the development process, a series of technical platforms such as QPCR, ddPCR, RAA-PCR, LAMP-PCR and the like are derived according to the mode of polymerase chain reaction.
The published application with the application number of CN202010441661.3 discloses an isothermal amplification-based integrated nucleic acid extraction and amplification detection system, wherein a lysis solution and magnetic beads are preloaded in a nucleic acid extraction cavity; pre-filling the first cleaning liquid, the second cleaning liquid and the third cleaning liquid in the first cleaning liquid cavity, the second cleaning liquid cavity and the third cleaning liquid cavity respectively; pre-loading the eluent in an eluent cavity; the isothermal amplification reaction solution is pre-loaded in an isothermal amplification chamber to construct a kit for extracting and detecting nucleic acid. Firstly, adding a target sample into a nucleic acid extraction cavity, and putting a bag type biochip into a control detector, wherein a push rod controller I, a push rod controller II, a push rod controller III, a push rod controller IV, a push rod controller V, a push rod controller IV and a push rod controller IV correspond to a nucleic acid extraction cavity, a nucleic acid washing liquid cavity I, a washing liquid cavity II, a washing liquid cavity III, an elution liquid cavity, a waste liquid cavity and an isothermal amplification cavity respectively; the controllable alternating electromagnets are arranged at two sides of the nucleic acid extraction cavity; the fluorescence detector corresponds to the reaction liquid collecting cavity; a temperature controller and No. two temperature-zones that the temperature controller corresponds nucleic acid extraction chamber and isothermal amplification chamber respectively, realization nucleic acid that can be quick accurate detects, can avoid the nucleic acid cross contamination who detects time measuring simultaneously and reduce biological safety risk, but nucleic acid extraction chamber, a washing sap cavity, No. two washing sap cavities, No. three washing sap cavities, the eluant chamber, waste liquid chamber, isothermal amplification chamber realize the propelling movement liquid through corresponding push rod controller respectively, consequently, there are structural component many, equipment is bulky, the shortcoming that the cost is high.
SUMMERY OF THE UTILITY MODEL
The present invention is directed to a connecting structure of an extraction layer and an amplification layer of a nucleic acid detecting system, which solves at least one of the problems of the prior art.
The utility model adopts the following technical scheme:
the utility model provides a nucleic acid detecting system's extraction layer and amplification layer connection structure, includes extraction layer, sealed rotation connection the waste liquid layer of extraction layer below, with the sealed fixed connection's of waste liquid layer amplification layer and push the piston assembly of liquid, be equipped with a plurality of liquid flow channels on extraction layer and the waste liquid layer, extraction layer and the amplification layer and with the air current passageway that liquid flow channel cooperation was used, through rotating the extraction layer and selectively with the liquid flow channel and the air current passageway intercommunication of extraction layer and amplification layer.
Further, the extraction layer comprises an extraction container, a sealing cover fixedly connected with the top of the extraction container and a sealing plate rotatably connected with the bottom of the extraction container.
Furthermore, an inner through hole is formed in the sealing cover, a plurality of outer through holes are formed in the edge of the sealing cover, and a short groove is formed in the top of the sealing cover.
Further, the bottom of the extraction container is fixedly connected with a sliding column, and the top of the sealing plate is provided with an annular sliding groove with a notch, which is matched with the sliding column; a clamping column is arranged at the edge of the sealing plate, a semicircular clamping groove matched with the clamping column is fixedly connected inside the waste liquid layer, and the clamping column is matched with the semicircular clamping groove to prevent the sealing plate from rotating together when the sliding column rotates in the annular sliding groove; the bottom wall of the extraction container is provided with a waste liquid hole, the edge of the sealing plate is provided with a plurality of waste liquid grooves matched with the waste liquid hole for use, the waste liquid grooves are communicated with the inner cavity of the waste liquid layer, and the waste liquid hole and the waste liquid grooves are communicated to form a liquid flow channel between the extraction layer and the waste liquid layer.
Further, the lateral wall of the extraction container is provided with an inner bulge, the inner bulge is provided with a side through hole matched with the outer through hole, and the side through hole is communicated with the inner cavity of the waste liquid layer.
Further, the amplification layer comprises a plurality of amplification pipes which are uniformly distributed in the circumferential direction, a bottom through hole is formed in the sealing plate, a middle protruding connecting portion is arranged on the waste liquid layer, a plurality of clamping holes communicated with the amplification pipes are formed in the connecting portion, the bottom through hole is communicated with the clamping holes to be matched with the clamping holes for use, and the bottom through hole is communicated with the clamping holes to form a liquid flow channel between the extraction layer and the amplification layer.
Furthermore, the bottom of the sealing plate is provided with a bottom air groove which communicates the corresponding amplification tube with the waste liquid layer.
The utility model has the advantages as follows:
the utility model discloses a draw layer, sealed rotation connect in draw the waste liquid layer of layer below, with waste liquid layer sealed fixed connection's the augmentation layer and push the piston assembly of liquid, through rotating draw layer selectively will draw the layer and correspond the liquid flow channel and the air current channel intercommunication that augment the pipe, promote the piston assembly at middle part and make and draw the intraformational liquid flow in the corresponding augmentation pipe, the utility model discloses a part structure is few, arranges the compactness, and is small, the simple operation, the preparation is convenient, has reduced the cost of manufacture.
Drawings
FIG. 1 is a schematic view of a vertical end face structure of a nucleic acid detecting system;
fig. 2 is a schematic perspective view of the sealing cover of the present invention;
fig. 3 is a schematic perspective view of the extraction container of the present invention 1;
fig. 4 is a schematic perspective view of the extraction container of the present invention, fig. 2;
fig. 5 is a schematic perspective view of a sealing plate of the present invention, illustrating a structure of fig. 1;
fig. 6 is a schematic perspective view of a sealing plate of the present invention, which is shown in fig. 2;
FIG. 7 is a schematic perspective view of the waste liquid layer of the present invention;
fig. 8 is a schematic perspective view of the amplification layer of the present invention.
Shown in the figure: 1-top cover, 2-preassembly layer, 3-sealing cover, 3001-inner through hole, 3002-short groove, 3003-outer through hole, 4-extraction container, 4001-inner protrusion, 4002-side through hole, 4003-liquid injection hole, 4004-waste liquid hole, 4005-sliding column, 5-sealing plate, 5001-annular sliding groove, 5002-bottom through hole, 5003-waste liquid groove, 5004-clamping column, 5005-bottom gas groove, 6-waste liquid layer, 6001-semicircular clamping groove, 6002-connecting part, 6003-clamping hole, 7-amplification layer, 7001-amplification tube, 8-rubber plug and 9-piston component.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention more clearly understood, the present invention will be described in further detail with reference to the accompanying drawings and detailed description. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than those specifically described herein, and therefore the scope of the present invention is not limited by the specific embodiments disclosed below.
The technical solution of the present invention will be further explained with reference to the accompanying drawings.
As shown in FIGS. 1 to 8, a connecting structure of an extraction layer and an amplification layer 7 of a nucleic acid detection system comprises the extraction layer, a waste liquid layer 6 connected below the extraction layer in a sealing and rotating manner, the amplification layer 7 fixedly connected with the waste liquid layer 6 in a sealing manner and a piston assembly 9 pushing liquid, wherein a plurality of liquid flow channels and air flow channels matched with the liquid flow channels are arranged on the extraction layer and the waste liquid layer 6, the extraction layer and the amplification layer 7, and the extraction layer is selectively communicated with the liquid flow channels and the air flow channels of the waste liquid layer 7 by rotating the extraction layer.
The extraction layer comprises an extraction container 4, a sealing cover 3 fixedly connected with the top of the extraction container 4 and a sealing plate 5 rotatably connected with the bottom of the extraction container 4. An inner through hole 3001 is formed in the sealing cover 3, a plurality of outer through holes 3003 are formed in the edge of the sealing cover 3, and a short groove 3002 is formed in the top of the sealing cover 3.
The bottom of the extraction container 4 is fixedly connected with a sliding column 4005, and the top of the sealing plate 5 is provided with an annular sliding groove 5001 which is matched with the sliding column 4005 and is provided with a notch; a clamping column 5004 is arranged at the edge of the sealing plate 5, a semicircular clamping groove 6001 matched with the clamping column 5004 for use is fixedly connected inside the waste liquid layer 6, and the clamping column 5004 is matched with the semicircular clamping groove 6001 to prevent the sealing plate 5 from rotating together when the sliding column 4005 rotates in the annular sliding groove 5001; draw and seted up waste liquid hole 4004 on the diapire of container 4, 4 waste liquid groove 5003 of the edge of closing plate 5 cooperation use with waste liquid hole 4004 are seted up, waste liquid groove 5003 and the inner chamber intercommunication of waste liquid layer 6, and waste liquid hole 4004 and waste liquid groove 5003 intercommunication form the flow channel between extraction layer and the waste liquid layer 6.
The side wall of the extraction container 4 is provided with an inner protrusion 4001, the inner protrusion 4001 is provided with a side through hole 4002 matched with the outer through hole 3003, and the side through hole 4002 is communicated with the inner cavity of the waste liquid layer 6.
The amplification layer 7 comprises 6 amplification tubes 7001 which are circumferentially and uniformly distributed, a bottom through hole is formed in the sealing plate 5, the waste liquid layer 6 is provided with a connecting part 6002 with a middle protruding part, the connecting part 6002 is provided with a plurality of clamping holes 6003 communicated with the amplification tubes 7001, the bottom through hole 5002 is communicated with the clamping holes 6003 to be matched for use, and the bottom through hole 5002 is communicated with the clamping holes 6003 to form a liquid flow channel between the extraction layer and the amplification layer 7. The bottom of the sealing plate 5 is provided with a bottom air groove 5005, and the bottom air groove 5005 communicates the corresponding amplification tube 7001 with the waste liquid layer 6, so that the amplification tube 7001 communicates with the atmosphere.
The gas exchange groove at the bottom of the external preassembly layer is communicated with the inner through hole 3001 and the short groove 3002 to communicate the space below the piston with the extraction layer, at the moment, the liquid injection hole 4003, the bottom through hole 5002 and the clamping hole 6003 are also communicated to form a liquid flow channel between the extraction layer and the amplification layer 7, the bottom gas groove 5005, the inner cavity of the waste liquid layer 6, the side through hole 4002, the outer through hole 3003 and the external preassembly layer are communicated to form an air flow channel between the atmosphere and the amplification tube 7001, the piston moves downwards, the space below the piston and above the sealing cover 3 is communicated with the extraction layer, the pressure in the extraction layer is increased, the liquid in the extraction layer is extruded downwards to flow into the corresponding amplification tube 7001, and the gas in the corresponding amplification tube 7001 is discharged into the atmosphere. The sealing plate 5, extraction container 4, sealing cap 3 and external pre-assembly layer 2 move downward every 60 degrees of rotation to complete the injection of 6 amplification tubes 7001.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (4)
1. A connection structure of an extraction layer and an amplification layer of a nucleic acid detection system is characterized by comprising the extraction layer, a waste liquid layer connected below the extraction layer in a sealing and rotating mode, the amplification layer fixedly connected with the waste liquid layer in a sealing mode and a piston assembly pushing liquid, wherein a plurality of liquid flow channels and air flow channels matched with the liquid flow channels are arranged on the extraction layer, the waste liquid layer, the extraction layer and the amplification layer, and the extraction layer and the air flow channels of the amplification layer are selectively communicated by rotating the extraction layer; the extraction layer comprises an extraction container, a sealing cover fixedly connected with the top of the extraction container and a sealing plate rotatably connected with the bottom of the extraction container; the sealing cover is provided with an inner through hole, the edge of the sealing cover is provided with a plurality of outer through holes, and the top of the sealing cover is provided with a short groove; the amplification layer comprises a plurality of amplification pipes which are uniformly distributed in the circumferential direction, a bottom through hole is formed in the sealing plate, a connecting portion with a protruding middle portion is arranged on the waste liquid layer, a plurality of clamping holes communicated with the amplification pipes are formed in the connecting portion, the bottom through hole is communicated with the clamping holes to be matched with the amplification pipes for use, and the bottom through hole is communicated with the clamping holes to form a liquid flow channel between the extraction layer and the amplification layer.
2. The structure of claim 1, wherein the extraction layer and amplification layer of the nucleic acid detection system are connected to a slide column fixedly connected to the bottom of the extraction container, and the top of the sealing plate is provided with a notched annular chute that cooperates with the slide column; a clamping column is arranged at the edge of the sealing plate, a semicircular clamping groove matched with the clamping column is fixedly connected inside the waste liquid layer, and the clamping column is matched with the semicircular clamping groove to prevent the sealing plate from rotating together when the sliding column rotates in the annular sliding groove; the bottom wall of the extraction container is provided with a waste liquid hole, the edge of the sealing plate is provided with a plurality of waste liquid grooves matched with the waste liquid hole for use, the waste liquid grooves are communicated with the inner cavity of the waste liquid layer, and the waste liquid hole and the waste liquid grooves are communicated to form a liquid flow channel between the extraction layer and the waste liquid layer.
3. The structure of claim 1, wherein the extraction layer and amplification layer of the extraction container have an inner protrusion on a sidewall thereof, the inner protrusion having a side through hole for matching with the outer through hole, the side through hole being in communication with the inner cavity of the waste liquid layer.
4. The nucleic acid detecting system according to claim 1, wherein the sealing plate has a bottom gas groove formed at a bottom thereof, and the bottom gas groove communicates the amplification tube with the waste liquid layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022259445.5U CN214458016U (en) | 2020-10-13 | 2020-10-13 | Extraction layer and amplification layer connecting structure of nucleic acid detection system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202022259445.5U CN214458016U (en) | 2020-10-13 | 2020-10-13 | Extraction layer and amplification layer connecting structure of nucleic acid detection system |
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| CN214458016U true CN214458016U (en) | 2021-10-22 |
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| CN202022259445.5U Active CN214458016U (en) | 2020-10-13 | 2020-10-13 | Extraction layer and amplification layer connecting structure of nucleic acid detection system |
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Address after: 450000 4th floor, building 25, Liandong u Valley Industrial Park, northwest corner, intersection of Haihang East Road and 25th Street, Zhengzhou Economic and Technological Development Zone, Henan Province Patentee after: Henan Zhongjian Shian Biotechnology Co.,Ltd. Address before: 450000 4th floor, building 25, Liandong u Valley Industrial Park, northwest corner, intersection of Haihang East Road and 25th Street, Zhengzhou Economic and Technological Development Zone, Henan Province Patentee before: Henan Zhitai Biotechnology Co.,Ltd. |