CN214457872U - Extraction layer and waste liquid layer connecting structure of nucleic acid detection system - Google Patents
Extraction layer and waste liquid layer connecting structure of nucleic acid detection system Download PDFInfo
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- CN214457872U CN214457872U CN202022259547.7U CN202022259547U CN214457872U CN 214457872 U CN214457872 U CN 214457872U CN 202022259547 U CN202022259547 U CN 202022259547U CN 214457872 U CN214457872 U CN 214457872U
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Abstract
The utility model discloses a nucleic acid detecting system's extraction layer and waste liquid layer connection structure, including extraction layer, sealed rotation connect the waste liquid layer of extraction layer below and push the piston assembly of liquid, be equipped with a plurality of liquid flow channels on extraction layer and the waste liquid layer and with the air current passageway that liquid flow channel cooperation was used, through rotating the extraction layer and selectively with the liquid flow channel and the air current passageway intercommunication of extraction layer and waste liquid layer. The utility model discloses a draw layer, sealed rotation and connect the waste liquid layer in the below of drawing layer and push the piston assembly of liquid, draw the liquid flow channel and the airflow channel intercommunication of layer and waste liquid layer through rotating the container and sealed lid of drawing selectively, the piston assembly who promotes the middle part makes and draws in situ liquid and flow in the waste liquid layer, realizes drawing layer selectivity and waste liquid layer intercommunication, and its part structure is few, arranges the compactness, and is small, and the preparation is convenient, has reduced the cost of manufacture.
Description
Technical Field
The utility model relates to a nucleic acid testing equipment technical field, in particular to nucleic acid detecting system's extraction layer and waste liquid layer connection structure.
Background
Laboratory tests are currently an important means for screening and diagnosing pathogenic bacteria or virus infections, and mainly comprise culture tests, microscopic tests, immunological tests and nucleic acid tests. The microscopic examination method is low in cost, simple and convenient, but has low positive rate and is easy to miss examination; the result reliability of the culture method is high, but the period is long and the cost is high; the immunological detection is simple to operate, but the detection is easy to miss due to a window period, and false positive results are often caused due to insufficient specificity of the antigen and the antibody.
The nucleic acid detection mainly takes PCR detection as a main part, the PCR is a method for synthesizing specific DNA fragments in vitro by enzymatic synthesis, and the method comprises a cycle consisting of high-temperature denaturation, low-temperature annealing and room-temperature extension reaction, so that the target DNA can be rapidly amplified. The PCR technology is very simple, has the advantages of high sensitivity, high specificity, short detection time and the like, has permeated into various biological branch science and clinical laboratory, and has important practical application value in many scientific fields of molecular biology, medical research, life science, bioengineering, genetic engineering, disease diagnosis, forensic medicine, archaeology and the like. Meanwhile, in the development process, a series of technical platforms such as QPCR, ddPCR, RAA-PCR, LAMP-PCR and the like are derived according to the mode of polymerase chain reaction.
The utility model discloses an authorized utility model patent with application number CN201821540197.8 discloses nucleic acid extraction and amplification fluorescence detection system, including sample sampling device 100, kit conveyor 200, sample preprocessing device 300, amplification detection device 400, nucleic acid extraction element 500, the public liquid box of waste liquid 600 and arm system. The waste liquid common liquid box 600 includes a first layer accommodating part 610, a second layer accommodating part 620, and a stirring device 630. The first layer receiving portion 610 includes a plurality of chambers 611 that are not connected to each other. Each chamber 611 has at least one inlet. At least one chamber 611 is a waste collection chamber. The second layer receptacle 620 is disposed below the first layer receptacle 610. Second layer receptacle 620 is configured to hold waste liquid. Each waste liquid collecting chamber is communicated with the second layer accommodating part 620. The nucleic acid extraction and amplification fluorescence detection system can adapt to the requirements of individual medical treatment, and is applied to various medical institution occasions, timely diagnosis and the like. However, the disadvantages are also obvious, and the first layer of containing part, the second layer of containing part and the waste liquid collecting cavity all adopt the fixed and communicated structure, so the disadvantages of more structural components, non-compact structure and high manufacturing cost are caused.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a nucleic acid detecting system's extraction layer and waste liquid layer connection structure to solve at least one of the problem that exists among the foretell prior art.
The utility model adopts the following technical scheme:
the utility model provides a nucleic acid detecting system's extraction layer and waste liquid layer connection structure, includes extraction layer, sealed rotation connection and extracts the waste liquid layer of layer below and push the piston assembly of liquid, be equipped with a plurality of liquid flow channel on extraction layer and the waste liquid layer and with the air current passageway that liquid flow channel cooperation was used, through rotating the extraction layer selectively with the liquid flow channel and the air current passageway intercommunication of extraction layer and waste liquid layer.
Further, the extraction layer comprises an extraction container, a sealing cover fixedly connected with the top of the extraction container and a sealing plate rotatably connected with the bottom of the extraction container.
Furthermore, an inner through hole is formed in the sealing cover, a plurality of outer through holes are formed in the edge of the sealing cover, and a short groove is formed in the top of the sealing cover.
Further, the bottom of the extraction container is fixedly connected with a sliding column, and the top of the sealing plate is provided with an annular sliding groove with a notch, which is matched with the sliding column; a clamping column is arranged at the edge of the sealing plate, a semicircular clamping groove matched with the clamping column is fixedly connected inside the waste liquid layer, and the clamping column is matched with the semicircular clamping groove to prevent the sealing plate from rotating together when the sliding column rotates in the annular sliding groove; the bottom wall of the extraction container is provided with a waste liquid hole, the edge of the sealing plate is provided with a plurality of waste liquid grooves matched with the waste liquid hole for use, the waste liquid grooves are communicated with the inner cavity of the waste liquid layer, and the waste liquid hole and the waste liquid grooves are communicated to form a liquid flow channel between the extraction layer and the waste liquid layer.
Further, the lateral wall of the extraction container is provided with an inner bulge, the inner bulge is provided with a side through hole matched with the outer through hole, and the side through hole is communicated with the inner cavity of the waste liquid layer.
Further, a bottom through hole is formed in the sealing plate, the waste liquid layer is provided with a connecting portion protruding in the middle, a plurality of clamping holes are formed in the connecting portion, and the bottom through hole is communicated with the clamping holes to be matched with each other for use.
Further, the bottom of the sealing plate is provided with a bottom air groove.
The utility model has the advantages as follows:
the utility model discloses a draw layer, sealed rotation and connect the waste liquid layer in the draw layer below and push the piston assembly of liquid, through rotating the liquid flow channel and the airflow channel intercommunication that draw container and sealed lid selectivity will draw layer and waste liquid layer, the piston assembly who promotes the middle part makes and draws in situ liquid and flow into waste liquid layer, realizes drawing layer selectivity and waste liquid layer intercommunication, and its part structure is few, arranges the compactness, and is small, and the simple operation, the preparation is convenient, has reduced the cost of manufacture.
Drawings
FIG. 1 is a schematic view of a vertical end face structure of a nucleic acid detecting system;
fig. 2 is a schematic perspective view of the sealing cover of the present invention;
fig. 3 is a schematic perspective view of the extraction container of the present invention 1;
fig. 4 is a schematic perspective view of the extraction container of the present invention, fig. 2;
fig. 5 is a schematic perspective view of a sealing plate of the present invention, illustrating a structure of fig. 1;
fig. 6 is a schematic perspective view of a sealing plate of the present invention, which is shown in fig. 2;
fig. 7 is a schematic perspective view of the waste liquid layer of the present invention.
Shown in the figure: 1-top cover, 2-preassembly layer, 3-sealing cover, 3001-inner through hole, 3002-short groove, 3003-outer through hole, 4-extraction container, 4001-inner protrusion, 4002-side through hole, 4003-liquid injection hole, 4004-waste liquid hole, 4005-sliding column, 5-sealing plate, 5001-annular sliding groove, 5002-bottom through hole, 5003-waste liquid groove, 5004-clamping column, 5005-bottom gas groove, 6-waste liquid layer, 6001-semicircular clamping groove, 6002-connecting part, 6003-clamping hole, 7-piston component and 8-rubber plug.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention more clearly understood, the present invention will be described in further detail with reference to the accompanying drawings and detailed description. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than those specifically described herein, and therefore the scope of the present invention is not limited by the specific embodiments disclosed below.
The technical solution of the present invention will be further explained with reference to the accompanying drawings.
As shown in FIGS. 1 to 7, an extraction layer and waste liquid layer connecting structure of a nucleic acid detecting system includes an extraction layer, a waste liquid layer 6 hermetically and rotatably connected below the extraction layer, and a piston assembly 7 for pushing liquid.
The extraction layer comprises an extraction container 4, a sealing cover 3 fixedly connected with the top of the extraction container 4 and a sealing plate 5 rotatably connected with the bottom of the extraction container 4.
An inner through hole 3001 is formed in the sealing cover 3, a plurality of outer through holes 3003 are formed in the edge of the sealing cover 3, a short groove 3002 is formed in the top of the sealing cover 3, and a bottom air groove 5005 is formed in the bottom of the sealing plate 5.
The bottom of the extraction container 4 is fixedly connected with a sliding column 4005, and the top of the sealing plate 5 is provided with an annular sliding groove 5001 which is matched with the sliding column 4005 and is provided with a notch; the edge of closing plate 5 is equipped with card post 5004, and the inside fixedly connected with of waste liquid layer 6 cooperates semicircle draw-in groove 6001 of using with card post 5004, and card post 5004 prevents in the annular chute 5001 rotation of closing plate 5 together when slide column 4005 with the cooperation of semicircle draw-in groove 6001. Draw and seted up waste liquid hole 4004 on the diapire of container 4, 4 waste liquid groove 5003 of the edge of closing plate 5 cooperation use with waste liquid hole 4004 are seted up, waste liquid groove 5003 and the inner chamber intercommunication of waste liquid layer 6, and waste liquid hole 4004 and waste liquid groove 5003 intercommunication form the flow channel between extraction layer and the waste liquid layer 6.
The side wall of the extraction container 4 is provided with an inner protrusion 4001, the inner protrusion 4001 is provided with a side through hole 4002 matched with the outer through hole 3003, and the side through hole 4002 is communicated with the inner cavity of the waste liquid layer 6.
The bottom through hole 5002 has been seted up on the closing plate 5, and waste liquid layer 6 is equipped with the bellied connecting portion 6002 in middle part, has seted up 6 calorie of holes 6003 on the connecting portion, and bottom through hole 5002 uses with calorie of hole 6003 intercommunication cooperation.
The piston lower space and the extraction layer are communicated through an inner through hole 3001 and a short groove 3002 in the gas exchange groove at the bottom of the external preassembly layer, at the moment, a waste liquid hole 4004 and a corresponding waste liquid groove 5003 are also communicated to form a liquid flow channel between the extraction layer and the waste liquid layer 6, the side through hole 4002, the outer through hole 3003 and the external preassembly layer are communicated to form an air flow channel between the atmosphere and the waste liquid layer 6, the piston moves downwards, the space above the piston and the sealing cover 3 is communicated with the extraction layer, the pressure in the extraction layer is increased, the liquid in the extraction layer is extruded downwards to flow into the waste liquid layer 6, and the gas in the waste liquid layer 6 is discharged into the atmosphere. The waste liquid hole 4004 is communicated with the other waste liquid tank 5003 in turn by rotating the lift container 4 and the sealing cap 3 to form a plurality of liquid flow paths between the extraction layer and the waste liquid layer 6.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (4)
1. A connection structure of an extraction layer and a waste liquid layer of a nucleic acid detection system is characterized by comprising the extraction layer, the waste liquid layer which is connected below the extraction layer in a sealing and rotating way and a piston assembly which is pushed in liquid, the extraction layer and the waste liquid layer are provided with a plurality of liquid flow channels and air flow channels matched with the liquid flow channels, selectively communicating the extraction layer with the flow channel and the gas flow channel of the waste layer by rotating the extraction layer; the extraction layer comprises an extraction container, a sealing cover fixedly connected with the top of the extraction container and a sealing plate rotatably connected with the bottom of the extraction container; the sealing cover is provided with an inner through hole, the edge of the sealing cover is provided with a plurality of outer through holes, and the top of the sealing cover is provided with a short groove; the sealing plate is provided with a bottom through hole, the waste liquid layer is provided with a connecting part with a convex middle part, a plurality of clamping holes are formed in the connecting portion, and the bottom through holes are communicated with the clamping holes for matching use.
2. The structure of claim 1, wherein the extraction layer and the waste liquid layer of the nucleic acid detection system are connected together, and the bottom of the extraction container is fixedly connected with a slide column, and the top of the sealing plate is provided with an annular chute with a notch, which is matched with the slide column; a clamping column is arranged at the edge of the sealing plate, a semicircular clamping groove matched with the clamping column is fixedly connected inside the waste liquid layer, and the clamping column is matched with the semicircular clamping groove to prevent the sealing plate from rotating together when the sliding column rotates in the annular sliding groove; the bottom wall of the extraction container is provided with a waste liquid hole, the edge of the sealing plate is provided with a plurality of waste liquid grooves matched with the waste liquid hole for use, the waste liquid grooves are communicated with the inner cavity of the waste liquid layer, and the waste liquid hole and the waste liquid grooves are communicated to form a liquid flow channel between the extraction layer and the waste liquid layer.
3. The structure of claim 1, wherein the extraction layer and the waste layer of the nucleic acid detection system are connected together, and the side wall of the extraction container is provided with an inner protrusion, the inner protrusion is provided with a side through hole matched with the outer through hole, and the side through hole is communicated with the inner cavity of the waste layer.
4. The structure of claim 1, wherein the bottom of the sealing plate is provided with a bottom gas groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022259547.7U CN214457872U (en) | 2020-10-13 | 2020-10-13 | Extraction layer and waste liquid layer connecting structure of nucleic acid detection system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022259547.7U CN214457872U (en) | 2020-10-13 | 2020-10-13 | Extraction layer and waste liquid layer connecting structure of nucleic acid detection system |
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| Publication Number | Publication Date |
|---|---|
| CN214457872U true CN214457872U (en) | 2021-10-22 |
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| CN202022259547.7U Active CN214457872U (en) | 2020-10-13 | 2020-10-13 | Extraction layer and waste liquid layer connecting structure of nucleic acid detection system |
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Address after: 450000 4th floor, building 25, Liandong u Valley Industrial Park, northwest corner, intersection of Haihang East Road and 25th Street, Zhengzhou Economic and Technological Development Zone, Henan Province Patentee after: Henan Zhongjian Shian Biotechnology Co.,Ltd. Address before: 450000 4th floor, building 25, Liandong u Valley Industrial Park, northwest corner, intersection of Haihang East Road and 25th Street, Zhengzhou Economic and Technological Development Zone, Henan Province Patentee before: Henan Zhitai Biotechnology Co.,Ltd. |