CN206768084U - A kind of PCR plate with PCR lids - Google Patents
A kind of PCR plate with PCR lids Download PDFInfo
- Publication number
- CN206768084U CN206768084U CN201720555830.XU CN201720555830U CN206768084U CN 206768084 U CN206768084 U CN 206768084U CN 201720555830 U CN201720555830 U CN 201720555830U CN 206768084 U CN206768084 U CN 206768084U
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- CN
- China
- Prior art keywords
- pcr
- lids
- plate body
- pcr plate
- reaction tube
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Abstract
The utility model discloses a kind of PCR plate with PCR lids, including PCR plate body and two PCR lids, two PCR lids to be movably fixed on PCR plate body；The PCR plate body is provided with reaction tube, and the both sides of the PCR plate body are respectively and fixedly provided with hollow pipe, and two hollow pipes are respectively less than the half of PCR plate body length and positioned at the both ends of PCR plate body；The PCR plate body both ends are equipped with retainer ring；The PCR lids whole clearing, and PCR is covered and is respectively positioned on provided with multiple ring sets, the bottom surface of each ring set in same level；The PCR lids side is provided with rotary shaft, and PCR lids opposite side is provided with clip.The utility model can seal up each reaction tube, and each reaction tube can be separated, improve the experiment degree of accuracy of PCR reactions, operation for PCR reactions brings facility, and real-time monitored can be carried out to the experiment in reaction tube while sealing, the PCR plate body can be dismantled with PCR cap bore, and PCR lids have and can preferably seal each reaction tube.
Description
Technical field
The utility model belongs to PCR plate field, and in particular to a kind of PCR plate with PCR lids.
Background technology
PCR reaction technologies are that now current molecular biology is essential during research and medical clinic applicationses.PCR
Reaction is PCR (Polymerase chain reaction), and abbreviation PCR, PCR are a kind of expansions quick in vitro
Increase the method for specific gene or DNA sequence dna, therefore turn into the amplification in vitro method of gene again.And PCR plate is in PCR
In, mainly as primer, Taq DNA polymerase, dNTP, template nucleic acid, the Mg for participating in amplified reaction2+, buffer solution etc. carrying
Thing.Enter performing PCR reaction using PCR plate and be widely used in hereditary, biochemical, immune, medicine and other fields, be not only applicable to gene point
From, clone and the basic research such as nucleic acid sequence analysis, it may also be used for the diagnosis of disease or any place for having DNA, RNA.
In traditional PCR plate be usually 8 holes, 12 holes, 96 holes or 384 holes, in order to ensure experiment accuracy and preferably
The performance for realizing PCR plate, often need to be sealed in hole in use, traditional sealing means are entered using plug
Row sealing, but Kong Yue is more, used plug is also more, cumbersome using, and is brought for the operation of experiment
Inconvenience, and good experiment reaction effect is not up to without using plug, and sample is evaporated also environment can be made
Into influence；Nowadays there are some dedicated for covering the cover plate of PCR plate, although the envelope to PCR plate can be realized disposably
Close, but it is not separated each reacting hole, easily reaction is influenced each other, and influences the result of experiment, and cover
Plate always links together with PCR plate, occurs inconvenience unavoidably when carrying out some operations；And when being cleaned to PCR plate,
PCR plate and many cleaning dead angles of lid junction are inconvenient to be cleaned.
Utility model content
In view of the shortcomings of the prior art, the purpose of this utility model is to propose a kind of PCR plate with PCR lids, the PCR
Plate can seal up each reaction tube, and can separate each reaction tube, and the experiment for improving PCR reactions is accurate
Degree, facility is brought for the operation of PCR reactions, and real-time monitored can be carried out to the experiment in reaction tube while sealing,
The PCR plate body can be dismantled with PCR cap bore, and PCR lids have and can preferably seal each reaction tube.
To achieve the above object, technical scheme is used by the utility model：
A kind of PCR plate with PCR lids, the PCR plate include PCR plate body and two PCR lids, two PCR lids activities
It is fixed on PCR plate body；
The PCR plate body is provided with multiple reaction tubes in array arrangement, and the both sides of the PCR plate body are respectively and fixedly provided with
Hollow pipe, two hollow pipes are respectively less than the half of PCR plate body length and positioned at the both ends of PCR plate body；The PCR plate body
Both ends are equipped with retainer ring；
The PCR lids whole clearing, and PCR is covered provided with multiple ring sets corresponding with reaction tube, the bottom of each ring set
Face is respectively positioned in same level；The PCR lids side is provided with the rotary shaft of insertion hollow pipe, and PCR lids opposite side, which is provided with, to be used for
By the clip that PCR plate body and PCR lids are fixed.
It is further that the ring set is rubber sleeve, and the internal diameter of ring set is more than the diameter of reaction tube；PCR is covered PCR plate
When body covers, be quality of rubber materials ring set by the extruding of itself and PCR plate body, enhance closing of the ring set to reaction tube
Property, reduce the leakage and evaporation of sample in reaction tube.
It is further that the number that the PCR covers ring set is the half of the number of reaction tube on PCR plate body；Two
PCR lids together just cover all reaction tubes.
It is further that the number of the reaction tube is 96, the number for the ring set that each PCR is covered is 48.
It is further that, when two PCR lids are fixed on PCR plate body, the ring set that two PCR are covered is by reaction tube
Cover；It ensure that the closure of each reaction tube.
It is further that, when two PCR lids are fixed on PCR plate body, two PCR lids are in close contact；It is so that whole
Device is more compact.
The beneficial effects of the utility model are：
(1) the PCR lids in the utility model can cover all reaction tubes on PCR plate body, greatly strengthen each
The sealing of individual reaction tube, reduce the leakage and volatilization of sample in each reaction tube, improve the reaction of sample in reaction tube
Quality, it ensure that preferable experimental result in each reaction tube；And each ring set that PCR is covered separates each reaction tube
Open, avoid the sample in each reaction tube from being influenced each other by leaking with volatilization, substantially increase the experiment degree of accuracy, grasped for experiment
Bring many facilities；
(2) the PCR lid whole clearings, can be by transparent PCR lids to reaction when the sample in reaction tube is reacted
Process carry out Real Time Observation, ensure each reaction individually carry out while, also can Real Time Observation and record reaction tube in
Reacting phenomenon, the degree of accuracy of experiment and the control degree to experiment are substantially increased, facility is brought for experiment；
(3) the PCR plate body in of the present utility model can be dismantled with PCR lids, some laboratories done, if need not
PCR is covered, then can cover PCR and remove, avoid PCR lids from having an impact experimental implementation, be decreased in cleaning to the clear of PCR lids
Wash；When being cleaned to PCR plate body and PCR lids simultaneously, PCR plate body and PCR lids can be dismantled, and carried out
Individually cleaning, reduce the cleaning to PCR plate body and PCR lids junction dead angle；
(4) PCR in the utility model is covered when covering on PCR plate body, after being fixed by clip, then is passed through
Rubber band is bundled in two retainer rings, strengthens the fixation of PCR lids and PCR plate body；
(5) PCR in the utility model is stamped two, when in use, a PCR can be arbitrarily opened according to real needs
Lid so that the use of the PCR plate has randomness, avoids when adding sample, by the exposure of all reaction tubes in atmosphere；
While may be implemented in a reaction tube simultaneously and tested, sample is added in another reaction tube and enters performing PCR reaction, is ensured
Preferable experiment effect.
Brief description of the drawings
Fig. 1 is the structural representation that PCR is covered on PCR plate body in the utility model；
Fig. 2 is the structural representation for having individual PCR lids to open in the utility model；
Fig. 3 is the structural representation of PCR plate body in the utility model；
Fig. 4 is the top view of PCR plate body in the utility model；
Fig. 5 is the side view of PCR plate body in the utility model；
Fig. 6 is the structural representation that PCR is covered in the utility model.
In figure：1st, PCR plate body；2nd, PCR is covered；3rd, reaction tube；4th, hollow pipe；5th, retainer ring；6th, ring set；7th, rotary shaft；
8th, clip.
Embodiment
In order that the purpose, technical scheme and advantage of utility model are clearer, below in conjunction with the accompanying drawings to the utility model
It is further elaborated.
In embodiment, as shown in figs. 1 to 6, a kind of PCR plate with PCR lids, the PCR plate includes PCR plate body 1
With two PCR lids 2, two PCR lids 2 are movably fixed on PCR plate body 1；It is in battle array that the PCR plate body 1, which is provided with multiple,
The reaction tube 3 of arrangement is arranged, the both sides of the PCR plate body 1 are respectively and fixedly provided with hollow pipe 4, and two hollow pipes 4 are respectively less than PCR plate sheet
The half of the length of body 1 and the both ends for being located at PCR plate body 1；The both ends of PCR plate body 1 are equipped with retainer ring 5；The PCR lids
2 whole clearings, and PCR lids 2 are provided with multiple ring sets 6 corresponding with reaction tube 3, the bottom surface of each ring set 6 are respectively positioned on same
On horizontal plane；The side of PCR lids 2 is provided with the rotary shaft 7 of insertion hollow pipe 4, and the opposite side of PCR lids 2, which is provided with, to be used for PCR plate sheet
The clip 8 that body 1 is fixed with PCR lids 2.
As a kind of prioritization scheme of the present utility model, the ring set 6 is rubber sleeve, and the internal diameter of ring set 6 is more than reaction
The diameter of pipe 3；It is that the ring set 6 of quality of rubber materials is crowded with PCR plate body 1 by itself when PCR lids 2 cover PCR plate body 1
Pressure, enhances closure of the ring set 6 to reaction tube 3, reduces the leakage and evaporation of sample in reaction tube 3, also by each reaction
Pipe 3 is mutually isolated, improves the quality of the PCR reactions in reaction tube 3.
As a kind of prioritization scheme of the present utility model, the number of the upper ring cap 6 of PCR lids 2 is to be reacted on PCR plate body
The half of the number of pipe 3；Two PCR lids 2 cover implementation seal isolation to all reaction tubes 3 together.
As a kind of prioritization scheme of the present utility model, the number of the reaction tube 3 is 96, the ring on each PCR lids 2
The number of set 6 is 48.
As a kind of prioritization scheme of the present utility model, when two PCR lids 2 are fixed on PCR plate body 1, two PCR
Ring set 6 on lid 2 covers reaction tube 3；It ensure that the closure of each reaction tube 3.
As a kind of prioritization scheme of the present utility model, when two PCR lids 2 are fixed on PCR plate body 1, two PCR
Lid 2 is in close contact；So that whole device is more compact.
Two PCR lids 2 are first fixed on PCR plate body 1, PCR lids 2 pass through when specifically used by the utility model
Rotary shaft 4 is inserted in hollow pipe 7 and is fixed, and PCR lids 2 can be realized in PCR plate by rotation of the rotary shaft 4 in hollow pipe 7
Upset on body 1, PCR lids 2 are buckled on PCR plate body 1 by clip 8 and are fixed, while are scheduled on by rubber band bundle
Further stabilization will be carried out between PCR lids and PCR plate body 1 in two retainer rings 5, strengthen ring set 6 and the table of PCR plate body 1
The contact in face, strengthen sealing；Each ring set 6 on PCR lids 2 keeps apart each reaction tube 3, ensure that in reaction tube 3
The sealing of sample, reduce the loss that leakage comes with volatile zone, avoid that relative effect occurs between each reaction tube 6, improve
The quality of PCR reactions；
When the sample in reaction tube 6 is reacted, Real Time Observation can be carried out to course of reaction by transparent PCR lids 2,
The response situation inside each reaction tube 6 can be observed, also can Real Time Observation while ensureing that each reaction is individually carried out
Reacting phenomenon with record reaction tube 6, substantially increases the degree of accuracy of experiment and the control degree to experiment, is brought for experiment
It is convenient；
And PCR plate body 1 and PCR lids 2 can be dismantled, some laboratories are being done, if not needing PCR lids 2, can incited somebody to action
PCR lids 2 are removed, and avoid PCR lids 2 from having an impact experimental implementation, and the cleaning to PCR lids 2 is decreased in cleaning；
When being cleaned to PCR plate body 1 and PCR lids 2 simultaneously, PCR plate body 1 and PCR lids 2 can be dismantled,
And individually cleaning is carried out, reduce the cleaning to PCR plate body 1 and the junction dead angle of PCR lids 2.
The advantages of general principle of the present utility model, principal character and the utility model has been shown and described above.One's own profession
The technical staff of industry is it should be appreciated that the utility model is not restricted to the described embodiments, described in above-described embodiment and specification
Simply illustrate principle of the present utility model, on the premise of the spirit and scope of the utility model is not departed from, the utility model is also
Various changes and modifications are had, these changes and improvements are both fallen within claimed the scope of the utility model.The utility model
Claimed scope is by appended claims and its equivalent thereof.
Claims (6)
- A kind of 1. PCR plate with PCR lids, it is characterised in that：The PCR plate includes PCR plate body (1) and two PCR lids (2), Two PCR lids (2) are movably fixed on PCR plate body (1)；The PCR plate body (1) is provided with multiple reaction tubes (3) in array arrangement, and the both sides of the PCR plate body (1) are equal Hollow pipe (4) is fixed with, two hollow pipes (4) are respectively less than the half of PCR plate body (1) length and positioned at PCR plate body (1) Both ends；PCR plate body (1) both ends are equipped with retainer ring (5)；The PCR covers (2) whole clearing, and PCR lids (2) are provided with multiple ring sets (6) corresponding with reaction tube (3), each The bottom surface of ring set (6) is respectively positioned in same level；Described PCR lids (2) side is provided with the rotary shaft (7) of insertion hollow pipe (4), PCR lid (2) opposite sides, which are provided with, to be used for the fixed clip (8) of PCR plate body (1) and PCR lids (2).
- 2. PCR plate according to claim 1, it is characterised in that：The ring set (6) is rubber sleeve, and the internal diameter of ring set (6) More than the diameter of reaction tube (3).
- 3. PCR plate according to claim 2, it is characterised in that：The number of PCR lid (2) upper ring caps (6) is PCR plate The half of the number of reaction tube (3) on body (1).
- 4. PCR plate according to claim 3, it is characterised in that：The number of the reaction tube (3) is 96, each PCR lids (2) number of the ring set (6) on is 48.
- 5. PCR plate according to claim 4, it is characterised in that：When two PCR lids (2) are fixed on PCR plate body (1) When, the ring set (6) that two PCR are covered on (2) covers reaction tube (3).
- 6. PCR plate according to claim 5, it is characterised in that：When two PCR lids (2) are fixed on PCR plate body (1) When, two PCR lids (2) are in close contact.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720555830.XU CN206768084U (en) | 2017-05-18 | 2017-05-18 | A kind of PCR plate with PCR lids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720555830.XU CN206768084U (en) | 2017-05-18 | 2017-05-18 | A kind of PCR plate with PCR lids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206768084U true CN206768084U (en) | 2017-12-19 |
Family
ID=60641715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720555830.XU Expired - Fee Related CN206768084U (en) | 2017-05-18 | 2017-05-18 | A kind of PCR plate with PCR lids |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206768084U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109439529A (en) * | 2018-12-29 | 2019-03-08 | 中国人民解放军军事科学院军事医学研究院 | A kind of reaction tube carrying out multiple nucleic acid amplification |
| CN114214169A (en) * | 2021-11-28 | 2022-03-22 | 美东汇成生命科技(昆山)有限公司 | Cross infection prevention PCR plate |
| CN114214168A (en) * | 2021-11-19 | 2022-03-22 | 美东汇成生命科技(昆山)有限公司 | PCR 96 board that practicality is high |
-
2017
- 2017-05-18 CN CN201720555830.XU patent/CN206768084U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109439529A (en) * | 2018-12-29 | 2019-03-08 | 中国人民解放军军事科学院军事医学研究院 | A kind of reaction tube carrying out multiple nucleic acid amplification |
| CN114214168A (en) * | 2021-11-19 | 2022-03-22 | 美东汇成生命科技(昆山)有限公司 | PCR 96 board that practicality is high |
| CN114214169A (en) * | 2021-11-28 | 2022-03-22 | 美东汇成生命科技(昆山)有限公司 | Cross infection prevention PCR plate |
| WO2023093877A1 (en) * | 2021-11-28 | 2023-06-01 | 美东汇成生命科技(昆山)有限公司 | Pcr plate for preventing cross infection |
| CN114214169B (en) * | 2021-11-28 | 2023-10-13 | 美东汇成生命科技(昆山)有限公司 | PCR plate for preventing cross infection |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171219 Termination date: 20180518 |