CN207047253U - A kind of culture device of dermis fibroblast - Google Patents
A kind of culture device of dermis fibroblast Download PDFInfo
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- CN207047253U CN207047253U CN201720865072.1U CN201720865072U CN207047253U CN 207047253 U CN207047253 U CN 207047253U CN 201720865072 U CN201720865072 U CN 201720865072U CN 207047253 U CN207047253 U CN 207047253U
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- blake bottle
- blake
- bottleneck
- slip lid
- bottle body
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Abstract
The utility model discloses a kind of culture device of dermis fibroblast, including cultivating bottle body, the culture bottle body includes blake bottle bottleneck, blake bottle bottleneck, blake bottle body and dividing plate, one end of the blake bottle body is provided with acclivitous blake bottle bottleneck, the other end of the blake bottle bottleneck is provided with blake bottle bottleneck, the centre position of the blake bottle body interior is provided with the dividing plate parallel with blake bottle bottleneck opening direction, two complete spaces of size are divided into by bottle body is cultivated by dividing plate, when needing contrast observation of the different nutrient solutions to the dermis fibroblast condition of production, the uniformity of its dependent variable can be ensured, and the observation in incubation to cell mass is more convenient, differential effect is more obvious；There is provided blake bottle slip lid, when being sampled to dermis fibroblast, it is only necessary to open blake bottle slip lid by slip lid open strip, make sampling more simple and convenient.
Description
Technical field
The utility model belongs to cell culture correlative technology field, and in particular to a kind of culture of dermis fibroblast
Equipment.
Background technology
Cell clone technology is also in cell culture, and the regular noun in biology is cell culture technology.No matter for
For whole biotechnology, or the biological cloning technology of one of them, cell culture is all an essential mistake
Journey, the extensive clone of cell culture inherently cell.Cell culture technology can by a cell by mass propgation into
For many cells that are simple unicellular or seldom breaking up, this is the essential link of clone technology, and cell culture is in itself
It is exactly the clone of cell.Substantial amounts of cell or its metabolite are obtained by cell culture.Because biological product is all from cell
Get, it is possible to which it is most crucial, most basic technology in biotechnology to say cell culture technology.During cell culture
Need to use cell cultivation equipment, therefore, need to use cell culture to set during to dermis fibroblast
It is standby
However, there is problems with the culture device of the dermis fibroblast of prior art in use：1、
, it is necessary to contrast the difference that dermis fibroblast is survived in different culture media in the incubation of dermis fibroblast
The opposite sex, prior art is needed after being cultivated using multiple Tissue Culture Flasks, then carries out paired observation, so in incubation
The phase same sex of its dependent variable can not be ensured, certain error be present so as to cause to observe result；2nd, the culture device of prior art exists
, it is necessary to which culture bottle cap is turned on when being sampled, then it is sampled, operation is more complicated, and samples difficult.
Utility model content
The purpose of this utility model is to provide a kind of culture device of dermis fibroblast, to solve above-mentioned background
The problems such as error and difficult sampling be present in the observation result proposed in technology.
To achieve the above object, the utility model provides following technical scheme：A kind of culture of dermis fibroblast
Equipment, including culture bottle body, the culture bottle body include blake bottle bottleneck, blake bottle bottleneck, blake bottle body and dividing plate,
One end of the blake bottle body is provided with acclivitous blake bottle bottleneck, and the other end of the blake bottle bottleneck is provided with training
Bottle bottleneck is supported, the centre position of the blake bottle body interior is provided with the dividing plate parallel with blake bottle bottleneck opening direction, institute
State and blake bottle slip lid is provided with above blake bottle body, culture bottle cap is provided with the blake bottle bottleneck.
Preferably, two sides of the blake bottle body are provided with slip lid neck with the position that blake bottle slip lid is in contact,
The blake bottle slip lid is engaged and installed in the top of blake bottle body by slip lid neck.
Preferably, the described one end of the upper surface of blake bottle slip lid away from blake bottle bottleneck is provided with slip lid open strip.
Preferably, the position that the blake bottle slip lid is in contact with dividing plate is provided with dividing plate neck, and the dividing plate is fastened on
The inside of dividing plate neck.
Preferably, the inclined angle of blake bottle bottleneck is 30 degree.
Preferably, the culture bottle cap engages connection with blake bottle bottleneck by thread.
Preferably, the dividing plate is divided into two identical spaces of size by bottle body is cultivated.
Compared with prior art, the beneficial effects of the utility model are：
1st, the utility model is divided into two complete spaces of size, it is necessary to be contrasted by dividing plate by bottle body is cultivated
During observation of the different nutrient solutions to the dermis fibroblast condition of production, it is ensured that the uniformity of its dependent variable, and
Observation in incubation to cell mass is more convenient, and differential effect is more obvious；
2nd, the utility model is provided with blake bottle slip lid, when being sampled to dermis fibroblast, it is not necessary to twists
Open culture bottle cap, it is only necessary to blake bottle slip lid is opened by slip lid open strip, makes sampling more simple and convenient.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model；
Fig. 2 is the structural representation of the utility model blake bottle body；
Fig. 3 is the mplifying structure schematic diagram of the utility model blake bottle slip lid；
In figure：1- slip lids open strip, 2- blake bottles slip lid, 21- dividing plates neck, 3- culture bottle cap, 4- blake bottles bottleneck,
5- blake bottles bottleneck, 6- cultures bottle body, 7- blake bottles body, 71- slip lids neck, 8- dividing plates.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment obtained, belong to the scope of the utility model protection.
Fig. 1-3 are referred to, the utility model provides a kind of technical scheme：A kind of culture of dermis fibroblast is set
It is standby, including culture bottle body 6, cultivating bottle body 6 includes blake bottle bottleneck 4, blake bottle bottleneck 5, blake bottle body 7 and dividing plate 8,
One end of blake bottle body 7 is provided with acclivitous blake bottle bottleneck 5, and the other end of blake bottle bottleneck 5 is provided with blake bottle
Bottleneck 4, cultivate the centre position inside bottle body 6 and be provided with the dividing plate 8 parallel with the opening direction of blake bottle bottleneck 4, blake bottle
The top of body 7 is provided with blake bottle slip lid 2, and culture bottle cap 3 is provided with blake bottle bottleneck 4.
It is sampled for convenience, in the utility model, it is preferred that two sides of blake bottle body 7 and blake bottle slip lid 2
The position being in contact is provided with slip lid neck 71, and blake bottle slip lid 2 is engaged and installed in blake bottle body 7 by slip lid neck 71
Top.
Blake bottle slip lid 2 is opened for convenience, in the utility model, it is preferred that the upper surface of blake bottle slip lid 2 is remote
One end of blake bottle bottleneck 4 is provided with slip lid open strip 1.
In order to ensure to cultivate the sealing inside bottle body 6, in the utility model, it is preferred that blake bottle slip lid 2 and dividing plate
8 positions being in contact are provided with dividing plate neck 21, and dividing plate 8 is fastened on the inside of dividing plate neck 21.
In the utility model, it is preferred that 5 inclined angle of blake bottle bottleneck is 30 degree.
In the utility model, it is preferred that culture bottle cap 3 engages connection with blake bottle bottleneck 4 by thread.
In order to ensure the uniformity of its dependent variable, in the utility model, it is preferred that dividing plate 8 is divided into bottle body 6 is cultivated
Two identical spaces of size.
Operation principle and process for using of the present utility model：After the utility model installs, corium is being carried out into fiber
Before like cell culture, reply culture bottle body 6 carries out disinfection processing, it is ensured that after the good seal of blake bottle slip lid 2, turns on blake bottle
Bottle cap 3, different dermis fibroblast nutrient solutions is added separately to cultivate in the two spaces inside bottle body 6, so
After replant into dermis fibroblast, then culture bottle cap 3 is tightened, is put into climatic chamber to corium into fiber-like
Cell is cultivated, it is necessary to when being sampled observation to dermis fibroblast, will cultivate bottle body 6 from climatic chamber
Take out, just like that blake bottle slip lid 2 is opened by slip lid open strip 1, using sampler respectively to the corium in different spaces
Fibroblast-like cellses are sampled observation, during culture, can also directly observe in culture bottle body 6 in two spaces
The size of cell mass.
While there has been shown and described that embodiment of the present utility model, for the ordinary skill in the art,
It is appreciated that these embodiments can be carried out with a variety of changes in the case where not departing from principle of the present utility model and spirit, repaiied
Change, replace and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.
Claims (7)
1. a kind of culture device of dermis fibroblast, including culture bottle body（6）, it is characterised in that：The blake bottle
Body（6）Including blake bottle bottleneck（4）, blake bottle bottleneck（5）, blake bottle body（7）And dividing plate（8）, the blake bottle body
（7）One end be provided with acclivitous blake bottle bottleneck（5）, the blake bottle bottleneck（5）The other end be provided with blake bottle
Bottleneck（4）, the culture bottle body（6）Internal centre position is provided with and blake bottle bottleneck（4）Opening direction it is parallel every
Plate（8）, the blake bottle body（7）Top be provided with blake bottle slip lid（2）, the blake bottle bottleneck（4）On be provided with training
Support bottle cap（3）.
A kind of 2. culture device of dermis fibroblast according to claim 1, it is characterised in that：The blake bottle
Body（7）Two sides and blake bottle slip lid（2）The position being in contact is provided with slip lid neck（71）, the blake bottle slip lid
（2）Pass through slip lid neck（71）It is engaged and installed in blake bottle body（7）Top.
A kind of 3. culture device of dermis fibroblast according to claim 1, it is characterised in that：The blake bottle
Slip lid（2）Upper surface away from blake bottle bottleneck（4）One end be provided with slip lid open strip（1）.
A kind of 4. culture device of dermis fibroblast according to claim 1, it is characterised in that：The blake bottle
Slip lid（2）With dividing plate（8）The position being in contact is provided with dividing plate neck（21）, the dividing plate（8）It is fastened on dividing plate neck（21）
Inside.
A kind of 5. culture device of dermis fibroblast according to claim 1, it is characterised in that：The blake bottle
Bottleneck（5）Inclined angle is 30 degree.
A kind of 6. culture device of dermis fibroblast according to claim 1, it is characterised in that：The blake bottle
Bottle cap（3）With blake bottle bottleneck（4）Engaged and connected by thread.
A kind of 7. culture device of dermis fibroblast according to claim 1, it is characterised in that：The dividing plate
（8）Bottle body will be cultivated（6）It is divided into two identical spaces of size.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720865072.1U CN207047253U (en) | 2017-07-17 | 2017-07-17 | A kind of culture device of dermis fibroblast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720865072.1U CN207047253U (en) | 2017-07-17 | 2017-07-17 | A kind of culture device of dermis fibroblast |
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| Publication Number | Publication Date |
|---|---|
| CN207047253U true CN207047253U (en) | 2018-02-27 |
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ID=61495772
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720865072.1U Active CN207047253U (en) | 2017-07-17 | 2017-07-17 | A kind of culture device of dermis fibroblast |
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| CN (1) | CN207047253U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109943485A (en) * | 2019-04-11 | 2019-06-28 | 广州赛泊泰生物技术有限公司 | Culture bacterium/cell shaking flask of active swap air |
-
2017
- 2017-07-17 CN CN201720865072.1U patent/CN207047253U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109943485A (en) * | 2019-04-11 | 2019-06-28 | 广州赛泊泰生物技术有限公司 | Culture bacterium/cell shaking flask of active swap air |
| CN109943485B (en) * | 2019-04-11 | 2023-11-21 | 江苏谱新生物医药有限公司 | Shaking bottle for actively exchanging air for culturing bacteria/cells |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: Unit A1, Building No. 1188, Lianhai Road, Minhang District, Shanghai 201100 Patentee after: SINONEURAL (SHANGHAI) CELL AND GENE ENGINEERING HOLDINGS Co.,Ltd. Address before: Room E321, No. 2200 Huancheng West Road, Fengxian District, Shanghai, 2010 Patentee before: HUAXIAYUAN (SHANGHAI) STEM CELL TECHNOLOGY CO.,LTD. |
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| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: Unit A2, 2 / F, building 23, No. 1188, LIANHANG Road, Minhang District, Shanghai 201100 Patentee after: Huaxiayuan Cell Engineering Group Co.,Ltd. Address before: Unit A1, Building No. 1188, Lianhai Road, Minhang District, Shanghai 201100 Patentee before: SINONEURAL (SHANGHAI) CELL AND GENE ENGINEERING HOLDINGS Co.,Ltd. |
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| CP03 | Change of name, title or address | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20211215 Address after: 201100 4th floor, building 23, No. 1188, LIANHANG Road, Minhang District, Shanghai Patentee after: Huaxiayuan (Shanghai) Biotechnology Co.,Ltd. Address before: Unit A2, 2 / F, building 23, No. 1188, LIANHANG Road, Minhang District, Shanghai 201100 Patentee before: Huaxiayuan Cell Engineering Group Co.,Ltd. |
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| TR01 | Transfer of patent right |