CN206902150U - Coliform single-tube quantitative detection means - Google Patents

Abstract

The utility model discloses the utility model and provides following technical scheme：Coliform single-tube quantitative detection means,Including culture apparatus and matched detection pipe,The culture apparatus includes culture tube,Liquid discharging tube,Valve,Handle and ventilative silica gel plug,The culture tube is fixedly installed liquid discharging tube close to the side of bottom,The liquid discharging tube connects with the inside of culture tube,The one end of the liquid discharging tube on the outside of culture tube is fixedly installed valve,Handle is fixedly installed above the valve,The mouth of pipe of the culture tube is plugged with ventilative silica gel plug,The surface side of the detection pipe scribbles the dense relation table of color bacterium,Coliform can be quantified using single tube,Multitube compared to MPN methods determines,Reduce detection workload and the usage amount of reagent,Liquid discharging tube can be realized to be sampled to the flora cultivated in culture tube,Need not be separately with sampling instruments such as pipettes,Reduce workload and the problem of bacterium solution pollution environment that sampling instrument is stained with.

Description

Coliform single-tube quantitative detection means

Technical field

It the utility model is related to coliform detection device technology field, specially coliform single-tube quantitative detection dress Put.

Background technology

The method of traditional detection coliform is lactose fermentation biochemical identification method, in recent years, can be produced using coliform Beta galactosidase hydrolysis O-Nitrophenylfluorone β-D- galactosides (ONPG) produce galactolipin and ortho-nitrophenol, become nutrient solution The principle of yellow and the coliform Fast Detection Technique to grow up, have been classified as American National Standard method, and had The commercialized product such as colilert.But up to the present, relevant beta galactosidase measure is all using the big of MPN methods detection Intestinal flora concentration, the multitube measure of MPN methods add detection workload and the usage amount of reagent.In coliform monitoring process The shortcomings such as complex steps, workload be big be present.

Utility model content

The purpose of this utility model is to provide coliform single-tube quantitative detection means, to solve in above-mentioned background technology The problem of proposition.

To achieve the above object, the utility model provides following technical scheme：Coliform single-tube quantitative detection means, bag Include culture apparatus and matched detection pipe, the culture apparatus includes culture tube, liquid discharging tube, valve, handle and thoroughly Aerosil plug, the culture tube are fixedly installed liquid discharging tube close to the side of bottom, and the inside of the liquid discharging tube and culture tube connects Logical, the one end of the liquid discharging tube on the outside of culture tube is fixedly installed valve, and handle is fixedly installed above the valve, described The mouth of pipe of culture tube is plugged with ventilative silica gel plug.

Further, the surface side of the detection pipe scribbles the dense relation table of color-bacterium, the opposite side of the detection pipe 7 Scribble at the 5mL being parallel to each other graduation mark at graduation mark and 6mL.

Further, the dense relation table of color-bacterium is provided with five, indicate respectively coliform concentration for 1/mL, 10/mL, 100/mL, 1000/mL and 10000/mL, and its color is deepened successively.

Further, the liquid discharging tube is arranged to the inverse u shape pipe of both ends open.

Further, the ventilative silica gel plug is internally provided with air-vent.

Compared with prior art, the beneficial effects of the utility model are：

1st, the utility model can be quantified using single tube to coliform, and the multitube compared to MPN methods determines, and reduces Detect workload and the usage amount of reagent.

2nd, liquid discharging tube can be realized is sampled to the flora cultivated in culture tube, it is not necessary to separately with sampling instruments such as pipettes, Reduce workload and the problem of bacterium solution pollution environment that sampling instrument is stained with.

3rd, liquid discharging tube replaces the aerogenesis situation of traditional Du Shi tubules observation coliform, and liquid discharging tube is suspended on culture tube In, there are certain intervals between culture bottom of the tube, solving Du Shi tubules and being sunken to test tube bottom may be blocked by sample, and make Bubble can not enter Du Shi tubules, cause false negative result.

When the 4th, preparing before experiment, valve can carry out tapping to liquid discharging tube and discharge bubble, solve Du Shi tubule bubbles It is difficult to drain net, causes ready culture medium to use, the problem of wasting reagent.Do not find that Du Shi is small even with preceding Bubble in pipe, the problem of causing false positive results after culture.

Brief description of the drawings

Fig. 1 is the utility model culture apparatus structure sectional view；

Fig. 2 is the utility model detection pipe structural representation.

In figure：1- culture apparatuses；2- culture tubes；3- liquid discharging tubes；4- valves；5- handles；6- breathes freely silica gel plug；7- is detected Pipe；8- colors-dense the relation table of bacterium；Graduation mark at 9-5mL；Graduation mark at 10-6mL.

Embodiment

Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made The every other embodiment obtained, belong to the scope of the utility model protection.

Fig. 1 and Fig. 2 are referred to, the utility model provides a kind of technical scheme：Coliform single-tube quantitative detection means, bag Include culture apparatus 1 and matched detection pipe 7, the culture apparatus 1 include culture tube 2, liquid discharging tube 3, valve 4, Hand 5 and ventilative silica gel plug 6, the culture tube 2 are fixedly installed liquid discharging tube 3, the liquid discharging tube 3 and culture close to the side of bottom The inside connection of pipe 2, the liquid discharging tube 3 are fixedly installed valve 4 in the one end in the outside of culture tube 2, and the valve 4 is fixed above Handle 5 is provided with, the mouth of pipe of the culture tube 2 is plugged with ventilative silica gel plug 6.

Referring to Fig. 2, the surface side of the detection pipe 7 scribbles the dense relation table 8 of color-bacterium, the detection pipe 7 it is another Side scribbles at the 5mL being parallel to each other graduation mark 10 at graduation mark 9 and 6mL.

For the ease of intuitively observing coliform, concentration, the dense relation table 8 of color-bacterium is provided with five, respectively Instruction coliform concentration is 1/mL, 10/mL, 100/mL, 1000/mL and 10000/mL, and its color is successively Deepen.

For the ease of liquid relief, the liquid discharging tube 3 is arranged to the inverse u shape pipe of both ends open.

For the ease of in the culture tube mouth of pipe, can effective ventilation, and prevent external microbe entrance, the ventilative silica gel plug 6 are internally provided with air-vent.

In use, load 20mL lauryl sulfate tryptose broths (LST) culture medium, turning handle toward culture tube 2 is interior Hand 5, valve 4 is opened, after liquid discharging tube 3 is full of LST culture mediums, swing handle 5, close valve 4.In the mouth of pipe of culture tube 2 beyond the Great Wall Ventilative silica gel plug 6, culture apparatus high pressure steam sterilization is standby.Then with aseptic manipulation, ventilative silica gel plug 6 is opened, toward training Support pipe 2 and add 1mL (g) measuring samples.Ventilative silica gel plug 6 beyond the Great Wall, after shaking up, is put in 36 ± 1 DEG C of incubators and cultivates 24 hours.

During detection, whether first observation has bubble generation in the liquid discharging tube 3 inside culture tube 2, can if bubble-free produces Report coliform feminine gender or 1/mL of coliform ＜；If there is bubble generation, following test then is carried out.

Then toward adding O-Nitrophenylfluorone β-D- galactosides (ONPG) that concentration is 0.5mg/mL in detection pipe 7 to 5mL Locate graduation mark 9, swing handle 5, open valve 4, the nutrient solution added into detection pipe 7 in culture tube 2 to graduation mark at 6mL 10 Place, shakes up.

The color that detection pipe 7 is observed after 2min is reacted, the color on relation table 8 dense with color-bacterium contrasts, and can obtain phase The coliform concentration answered.

When the dense relation table 8 of color-bacterium makes, first toward 20mL lauryl sulfate tryptose broth (LST) culture medium The middle bacterium solution 1mL for being inoculated with 1,10,100,1000,10000/mL coliforms respectively, culture 24 is small in 36 ± 1 DEG C of incubators When, respectively toward above-mentioned after addition 1mL cultures in the O-Nitrophenylfluorone β-D- galactosides (ONPG) that 5mL concentration is 0.5mg/mL Nutrient solution, shake up, the color of each pipe is observed after reaction 2min, face is made according to the color after different coliform concentration-responses The dense relation table 8 of color-bacterium.

Whole culture is simple and practical with detection process, is easy to promote.

While there has been shown and described that embodiment of the present utility model, for the ordinary skill in the art, It is appreciated that these embodiments can be carried out with a variety of changes in the case where not departing from principle of the present utility model and spirit, repaiied Change, replace and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.

Claims (5)

1. coliform single-tube quantitative detection means, including culture apparatus (1) and matched detection pipe (7), it is special Sign is：The culture apparatus (1) includes culture tube (2), liquid discharging tube (3), valve (4), handle (5) and ventilative silica gel plug (6), The culture tube (2) is fixedly installed liquid discharging tube (3), the liquid discharging tube (3) and the inside of culture tube (2) close to the side of bottom Connection, the one end of the liquid discharging tube (3) on the outside of culture tube (2) are fixedly installed valve (4), and the valve (4) is fixed above Handle (5) is provided with, the mouth of pipe of the culture tube (2) is plugged with ventilative silica gel plug (6).

2. coliform single-tube quantitative detection means according to claim 1, it is characterised in that：The detection pipe (7) Surface side scribbles the dense relation table of color-bacterium (8), and the opposite side of the detection pipe (7) scribbles graduation mark at the 5mL being parallel to each other (9) graduation mark (10) and at 6mL.

3. coliform single-tube quantitative detection means according to claim 2, it is characterised in that：The dense pass of color-bacterium Be that table (8) is provided with five, indicate respectively coliform concentration for 1/mL, 10/mL, 100/mL, 1000/mL and 10000/mL, and its color is deepened successively.

4. coliform single-tube quantitative detection means according to claim 1, it is characterised in that：The liquid discharging tube (3) sets It is set to the inverse u shape pipe of both ends open.

5. coliform single-tube quantitative detection means according to claim 1, it is characterised in that：The ventilative silica gel plug (6) it is internally provided with air-vent.