CN205443285U - Body fluid blake bottle - Google Patents

Body fluid blake bottle Download PDF

Info

Publication number
CN205443285U
CN205443285U CN201620126891.XU CN201620126891U CN205443285U CN 205443285 U CN205443285 U CN 205443285U CN 201620126891 U CN201620126891 U CN 201620126891U CN 205443285 U CN205443285 U CN 205443285U
Authority
CN
China
Prior art keywords
bottle
culture
culture bottle
reservoir
body fluid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201620126891.XU
Other languages
Chinese (zh)
Inventor
宋新妥
赵明辉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yunhui Technology Tianjin Co ltd
Original Assignee
Yunhui Technology Tianjin Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yunhui Technology Tianjin Co ltd filed Critical Yunhui Technology Tianjin Co ltd
Priority to CN201620126891.XU priority Critical patent/CN205443285U/en
Application granted granted Critical
Publication of CN205443285U publication Critical patent/CN205443285U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model discloses a body fluid blake bottle, including blake bottle and the indicator that fills the culture medium, the blake bottle includes the plug and the bottle lid of bottle, sealed bottle, is equipped with liquid medium and the mist of forming the microbial growth necessary condition in the bottle of blake bottle, is negative pressure sterile environment in the blake bottle, the indicator is an external transparent reservoir of plastics that has the minute hand, and there is a plastics lid reservoir top, and the lower half be the sleeve pipe be connected with the blake bottle, and long needle position central and communicate with each other with the reservoir in the sheathed tube. The utility model discloses, aerobic and amaerobic cultivation can be carried out simultaneously, the trouble of two blake bottles is avoided vaccinating to sample amount can be reduced, the problem that the volume of leading to the sample is few when divideing into two bottles with the sample problem and anaerobe culture are ignored is solved.

Description

A kind of body fluid culture bottle
Technical field
This utility model relates to the microbial cultivation device of a kind of Clinical Laboratory, specifically, is a kind of body fluid culture bottle.
Background technology
Increasing along with clinical bacteria infects the M & M caused, the most clinical body fluid is cultivated especially blood cultivation and is had been subjected to pay attention to.Such as increase to 3.1% in 2006 from 1.6% in 1986 at bloodstream infection (bacteremia, septicemia, sepsis etc.) sickness rate, case fatality rate reaches 21.0%-48.0%.But body fluid is cultivated and be there is also some problems at present, the not enough of sampling quantity affects growth of microorganism, the cultivation of anaerobe does not comes into one's own, when there is suspected infection in patient, the most first apply antibacterials, after antibacterials are invalid, just carry out aerobic cultivation, during aerobic cultivation feminine gender, just consider Anaerobic culturel.Current training method has biphase culturing bottle, single-phase culture bottle or auto culturing system, it is all generally aerobic bottle and anaerobism bottle uses simultaneously, the reason being likely to be due to each side such as sampling quantity, cost, resource in actual clinical only carries out aerobic bottle cultivation, carry out Anaerobic culturel again when aerobic bottle is come to nothing, this kind of way has the risk of delay treatment.
Utility model content
Technical problem to be solved in the utility model is, a kind of body fluid culture bottle is provided, aerobic and anaerobism cultivation can be carried out simultaneously, avoid inoculating the trouble of two culture bottles, and sampling quantity can be reduced, solve to cause, when sample is divided into two bottles, problem and the unheeded problem of anaerobic culture that sample size is few.
In order to solve above-mentioned technical problem, the technical solution adopted in the utility model is: a kind of body fluid culture bottle, including the culture bottle and the indicator that fill culture medium, the culture bottle of culture medium includes bottle, the plug of sealed bottle body and bottle cap, the bottle of culture bottle is built with being negative pressure gnotobasis in the composition fluid medium of growth of microorganism essential condition and mixed gas, culture bottle;Indicator is a plastic, transparent reservoir being circumscribed with minute hand, has a vinyl cover above reservoir, and reservoir lower half is the sleeve pipe being connected with culture bottle, and minute hand is positioned at the central authorities of sleeve pipe and communicates with reservoir.
Described culture bottle bottle is transparent cylindrical bottle, for vial or double-layer plastic bottle, it is ensured that bottle is transparent and airtight.
Described culture bottle plug is butyl rubber plug.
Described culture bottle bottle cap is aluminium lid, is removed by mid portion during inoculation.
Described reservoir is cylindrical transparent plastic body, the lower lumen of sleeve pipe and culture bottle bottle cap size coupling, makes minute hand insert in culture bottle, and the bottom of sleeve pipe is stuck in the outside of culture bottle bottle cap, makes reservoir contact firmly with culture bottle.
The beneficial effects of the utility model are: only using a culture bottle to cultivate, reduce sampling quantity, the microorganism that sample size deficiency can be avoided to cause is difficult to grow the false negative being likely to result in.Carry out aerobic cultivation and Anaerobic culturel simultaneously, solve present clinic and think little of the situation of Anaerobic culturel, improve the recall rate of anaerobe.Culture fluid, in reservoir, samples when facilitating transferred species, it is not necessary to open culture bottle, reduces and pollute, easy to operate.Use a culture bottle, do aerobic cultivation and Anaerobic culturel simultaneously, reduce workload, cost-effective.
Accompanying drawing explanation
Fig. 1 is the culture bottle structural representation of this utility model body fluid culture bottle.
Fig. 2 is the indicator structure schematic diagram of this utility model body fluid culture bottle.
Fig. 3 is the schematic diagram of indicator microoraganism growth after this utility model body fluid culture bottle is inoculated.
Fig. 4 is that after this utility model body fluid culture bottle is inoculated, indicator microoraganism grows another schematic diagram.
Fig. 5 is the indicator microoraganism another schematic diagram of growth after the inoculation of this utility model body fluid culture bottle.
Detailed description of the invention
With detailed description of the invention, this utility model is described in further detail below in conjunction with the accompanying drawings:
As shown in Figure 1, 2, body fluid culture bottle of the present utility model, including the culture bottle and the indicator that fill culture medium, the culture bottle filling culture medium includes bottle 1, the plug 3 of sealed bottle body and bottle cap 2, the bottle 1 of culture bottle is built with being negative pressure gnotobasis in the composition fluid medium of growth of microorganism essential condition and mixed gas, culture bottle;Indicator is a plastic, transparent reservoir 6 being circumscribed with minute hand 8, has a vinyl cover 7 above reservoir, and reservoir lower half is the sleeve pipe 9 being connected with culture bottle, and minute hand 8 is positioned at the central authorities of sleeve pipe 9 and communicates with reservoir 6.
Described culture bottle bottle 1 is transparent cylindrical bottle, for vial or double-layer plastic bottle, it is ensured that bottle is transparent and airtight.Described culture bottle plug 1 is butyl rubber plug.Described culture bottle bottle cap 2 is aluminium lid, is removed by mid portion during inoculation.
Described reservoir 6 is cylindrical transparent plastic body, the lower lumen of sleeve pipe 9 and culture bottle bottle cap 2 size coupling, makes minute hand 8 insert in culture bottle, and the bottom of sleeve pipe 9 is stuck in the outside of culture bottle bottle cap 2, makes reservoir contact firmly with culture bottle.
Fluid medium in culture bottle contains the nutrient substance promoting that aerobe and anaerobe grow, including basal nutrient material tryptic soy broth, brain heart infusion, yeast extract, saccharide etc..Including one or more microorganism risen in the microorganism B race of repair, vitamin K race, vitamin E race.Including one or more promoted in the sodium citrate of growth of microorganism, sodium citrate, ferric citrate, sodium sulfite, magnesium sulfate, arginine, L-cysteine hydrochloride etc..Including, reduce other materials interference in sample, promote one or more in the edetate of growth of microorganism, para-amino benzoic acid, poly-Fructus Foeniculi sodium sulfonate, glucoseoxidase, chlorhematin.
Mixed gas in culture bottle includes one or more in oxygen, nitrogen, hydrogen, carbon dioxide.
After being inoculated into by body fluid sample in culture bottle, the minute hand syringe needle of indicator is inserted in fluid medium by bottle stopper.When growth of microorganism produces gas, adding the gas pressure within culture bottle, now gas pressure makes fluid medium be entered in reservoir by syringe needle, if occurring culture fluid in reservoir, has growth of microorganism in indicating liquid culture medium.When growth certain time, along with the growth aerobe of microorganism runs out of the oxygen in culture bottle, then the anaerobe being suppressed can grow.
After there is culture fluid in reservoir, instruction has growth of microorganism, now can carry out transferred species and continue next step operation.During transferred species in an aseptic environment, the vinyl cover of reservoir is opened, draw culture fluid.The method is easy to operate, and does not affect microorganism continued growth in culture bottle.
Fluid medium includes:
Tryptic soy broth 1-50%, yeast extract 0.1-10%, brain heart infusion 0.1-10%, sucrose 0.1-10%, glucose 0.1-10%, sodium chloride 0.5-5%, para-amino benzoic acid 0.005-1%, poly-anetholesulfonic acid sodium 0.05-1%, sodium citrate 0.25-2.5%, arginine 0.25-2.5%, potassium dihydrogen phosphate 0.25-2.5%, vitamin 0.00005-1%, surplus is purified water.
Preferably:
Tryptic soy broth 2.5%, yeast extract 0.3%, brain heart infusion 0.1%, sucrose 0.2%, glucose 0.2%, sodium chloride 0.5%, para-amino benzoic acid 0.005%, poly-anetholesulfonic acid sodium 0.05%, sodium citrate 0.25%, arginine 0.25%, potassium dihydrogen phosphate 0.25%, vitamin 0.00065%, surplus is purified water.
Tryptic soy broth 3%, yeast extract 0.2%, brain heart infusion 0.1%, sucrose 0.25%, glucose 0.15%, sodium chloride 0.5%, para-amino benzoic acid 0.005%, poly-anetholesulfonic acid sodium 0.05%, sodium citrate 0.25%, arginine 0.25%, potassium dihydrogen phosphate 0.3%, vitamin 0.0005%, surplus is purified water.
Tryptic soy broth 2%, yeast extract 0.4%, brain heart infusion 0.1%, sucrose 0.25%, glucose 0.4%, sodium chloride 0.5%, para-amino benzoic acid 0.005%, poly-anetholesulfonic acid sodium 0.05%, sodium citrate 0.3%, arginine 0.25%, potassium dihydrogen phosphate 0.25%, vitamin 0.001%, surplus is purified water.
Mixed gas includes 5% carbon dioxide, 95% air.
Embodiment 1, the body fluid sample that will gather, by negative pressure in culture bottle, it is injected in culture bottle, the minute hand of indicator is inserted into inside fluid medium, reservoir vinyl cover is built.The gas produced when growth of microorganism in sample makes cultivation pressure of the inside of a bottle increase, and makes culture fluid pass through minute hand and enters reservoir, when occurring culture fluid in the reservoir of indicator, growth of microorganism (such as Fig. 3) has been described
Embodiment 2, is injected into the body fluid sample of collection in culture bottle, is inserted into inside fluid medium by the minute hand of indicator, and reservoir vinyl cover is built.The gas produced when growth of microorganism in sample makes cultivation pressure of the inside of a bottle increase, culture fluid is made to enter reservoir by minute hand, if it is indicated that the culture fluid in the reservoir of device is less, illustrate that microorganism belongs to the less Pseudomonas of gas production present in sample, the culture medium (such as Fig. 4) of transferred species can be selected according to this.
Embodiment 3, if it is indicated that the culture fluid in the reservoir of device is more, illustrates that microorganism belongs to the Pseudomonas that gas production is more present in sample, can select the culture medium (such as Fig. 5) of transferred species according to this.
Embodiment 4, if it is indicated that the culture fluid liquid level growth rate in device increases stage by stage or the most continuously, there may be more than one microorganism, transferred species cultivation can be carried out stage by stage, what the later stage increased is probably anaerobe, need timely transferred species, and carry out improving transferred species success rate under obligate anaerobic condition of culture.
Embodiment described above is merely to illustrate technological thought of the present utility model and feature, its object is to make those skilled in the art it will be appreciated that content of the present utility model implementing according to this, only can not limit the scope of the claims of the present utility model with the present embodiment, what the spirit disclosed in the most all this utility model was made changes on an equal basis or modifies, and still falls in the scope of the claims of the present utility model.

Claims (5)

1. a body fluid culture bottle, it is characterized in that, including the culture bottle and the indicator that fill culture medium, the culture bottle filling culture medium includes bottle (1), the plug (3) of sealed bottle body and bottle cap (2), indicator is a plastic, transparent reservoir (6) being circumscribed with minute hand (8), a vinyl cover (7) is had above reservoir, reservoir lower half is the sleeve pipe (9) being connected with culture bottle, and minute hand (8) is positioned at the central authorities of sleeve pipe (9) and communicates with reservoir (6).
Body fluid culture bottle the most according to claim 1, it is characterised in that described culture bottle bottle (1) is transparent cylindrical bottle, for vial or double-layer plastic bottle, it is ensured that bottle is transparent and airtight.
Body fluid culture bottle the most according to claim 1, it is characterised in that described culture bottle plug (1) is butyl rubber plug.
Body fluid culture bottle the most according to claim 1, it is characterised in that described culture bottle bottle cap (2) is aluminium lid, removes mid portion during inoculation.
Body fluid culture bottle the most according to claim 1, it is characterized in that, described reservoir (6) is cylindrical transparent plastic body, the lower lumen of sleeve pipe (9) and culture bottle bottle cap (2) size coupling, minute hand (8) is made to insert in culture bottle, the bottom of sleeve pipe (9) is stuck in the outside of culture bottle bottle cap (2), makes reservoir contact firmly with culture bottle.
CN201620126891.XU 2016-02-18 2016-02-18 Body fluid blake bottle Expired - Fee Related CN205443285U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201620126891.XU CN205443285U (en) 2016-02-18 2016-02-18 Body fluid blake bottle

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201620126891.XU CN205443285U (en) 2016-02-18 2016-02-18 Body fluid blake bottle

Publications (1)

Publication Number Publication Date
CN205443285U true CN205443285U (en) 2016-08-10

Family

ID=56581909

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201620126891.XU Expired - Fee Related CN205443285U (en) 2016-02-18 2016-02-18 Body fluid blake bottle

Country Status (1)

Country Link
CN (1) CN205443285U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105505752A (en) * 2016-02-18 2016-04-20 允汇科技(天津)有限公司 Body fluid culture bottle

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105505752A (en) * 2016-02-18 2016-04-20 允汇科技(天津)有限公司 Body fluid culture bottle

Similar Documents

Publication Publication Date Title
CN108034621A (en) A kind of enrichment method of pit mud anaerobism clostridium Clostridium
CN203211727U (en) Anaerobic transport box
CN205443285U (en) Body fluid blake bottle
CN110437973A (en) A kind of culture apparatus and cultural method of anaerobic bacteria
CN104789453A (en) Laboratory anaerobic immobilized fermentation device and anaerobic fermentation method
CN201890884U (en) Blood microorganism culture flask
CN203369920U (en) Sputum culture sputum suction device
CN103308360B (en) The viscosity reduction equipment of toughness clinical memory qu antized table and viscosity reducing process
CN211847925U (en) Culture medium nitrogen charging device
CN105505752A (en) Body fluid culture bottle
CN211853522U (en) Culture medium nitrogen filling needle
CN201395594Y (en) Culture bottle
CN204198747U (en) A kind of cell culture bags of convenient operation
CN102660459A (en) Anaerobic culture box and using method thereof
CN203772593U (en) Clostridium difficile infects sample sampling preliminary treatment mixing arrangement
CN207793267U (en) A kind of blood culture detection report male device
CN211420158U (en) Anaerobism biological sample shifts bottle
CN201648404U (en) Novel two-phase culture medium of bacillus tuberculosis typus bovinus
CN201574151U (en) Novel blood sample biphasic culture bottle
CN203346391U (en) Handmade blood culture bottle
CN110885873A (en) Reagent for blood culture bottle production and blood culture bottle production process
CN2603089Y (en) Large bore diameter fermentation inoculator
CN110437996A (en) A kind of small-sized aerobic bacteria culture apparatus and aerobic bacteria cultural method
CN219652999U (en) Microorganism triangular flask culture apparatus
CN204779580U (en) Simple and easy artificial rumen blake bottle

Legal Events

Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160810