CN105505752A - Body fluid culture bottle - Google Patents
Body fluid culture bottle Download PDFInfo
- Publication number
- CN105505752A CN105505752A CN201610093544.6A CN201610093544A CN105505752A CN 105505752 A CN105505752 A CN 105505752A CN 201610093544 A CN201610093544 A CN 201610093544A CN 105505752 A CN105505752 A CN 105505752A
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- CN
- China
- Prior art keywords
- bottle
- culturing bottle
- culture
- reservoir
- culturing
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
Abstract
The invention discloses a body fluid culture bottle. The body fluid culture bottle comprises a culture bottle body containing a culture medium and an indicator. The culture bottle body comprises a bottle body, a rubber plug and a bottle cap, wherein the rubber plug and the bottle cap are used for sealing the bottle body. The fluid culture medium and mixed gas for forming necessary conditions for growth of microorganisms are contained in the bottle body of the culture bottle body, and the interior of the culture bottle body is a negative pressure sterile environment. The indicator is a plastic transparent fluid reservoir externally connected with a long pointer, a plastic cap is arranged on the fluid reservoir, a sleeve connected with the culture bottle body is arranged on the lower half portion, and the long pointer is located in the center of the sleeve and communicated with the fluid reservoir. Aerobic and anaerobic culture can be conducted at the same time, the trouble of inoculation of two culture bottles is saved, the sampling quantity can be reduced, and the problem that samples are distributed in the two bottles, so that the sample capacity is small and the problem that anaerobic bacterium culture is neglected are solved.
Description
Technical field
The present invention relates to a kind of microbial cultivation device of Clinical Laboratory, specifically, is a kind of body fluid culturing bottle.
Background technology
Increase along with clinical bacteria infects the M & M caused, present clinical body fluid is cultivated especially blood cultivation and is come into one's own.Such as be increased to 3.1% in 2006 from 1.6% in 1986 at bloodstream infection (microbemia, septicemia, Sepsis etc.) sickness rate, case fatality rate reaches 21.0%-48.0%.But body fluid is cultivated and also be there are some problems at present, the deficiency of sampling quantity affects microorganism growth, the cultivation of anerobe does not come into one's own, when there is suspected infection in patient, often rule of thumb first apply antibacterials, after antibacterials are invalid, just carry out aerobic cultivation, when aerobic cultivation is negative, just consider Anaerobic culturel.Current training method has biphase culturing bottle, single-phase culturing bottle or auto culturing system, usually be all that aerobic bottle and anaerobism bottle use simultaneously, actual clinical may only carry out aerobic bottle cultivation due to the reason of each side such as sampling quantity, cost, resource, carry out Anaerobic culturel again when aerobic bottle is come to nothing, this kind of way has the risk of delay treatment.
Summary of the invention
Technical problem to be solved by this invention is, a kind of body fluid culturing bottle is provided, aerobic and cultivation that is anaerobism can be carried out simultaneously, avoid the trouble of inoculation two culturing bottles, and can sampling quantity be reduced, solve the problem and the unheeded problem of anaerobic bacteria culture that cause sample size few when sample being divided into two bottles.
In order to solve the problems of the technologies described above, the technical solution used in the present invention is: a kind of body fluid culturing bottle, comprise the culturing bottle and telltale that fill substratum, the culturing bottle of substratum comprises bottle, the plug of sealed bottle body and bottle cap, the bottle of culturing bottle, built with the liquid nutrient medium and the mixed gas that form microorganism growth prerequisite, is negative pressure gnotobasis in culturing bottle; Telltale is a plastic, transparent reservoir being circumscribed with minute hand, has a plastic cover above reservoir, and reservoir Lower Half is the sleeve pipe be connected with culturing bottle, and minute hand is positioned at the central authorities of sleeve pipe and communicates with reservoir.
Described culturing bottle bottle is transparent cylindrical bottle, is vial or double-layer plastic bottle, ensures that bottle is transparent and airtight.
Described culturing bottle plug is butyl rubber plug.
Described culturing bottle bottle cap is aluminium lid, is removed by middle portion during inoculation.
Described reservoir is cylindrical transparent plastic body, and the lower lumen of sleeve pipe and culturing bottle bottle cap size coupling, minute hand is inserted in culturing bottle, and the bottom of sleeve pipe is stuck in the outside of culturing bottle bottle cap, and reservoir is contacted firmly with culturing bottle.
The invention has the beneficial effects as follows: only use a culturing bottle to cultivate, reduce sampling quantity, the microorganism that sample size deficiency can be avoided to cause not easily grows the false negative that may cause.Carry out aerobic cultivation and Anaerobic culturel simultaneously, solve the clinical situation thinking little of Anaerobic culturel now, improve the recall rate of anerobe.Nutrient solution, in reservoir, facilitates during transferred species and samples, do not need to open culturing bottle, decreasing pollution, easy to operate.Use a culturing bottle, do aerobic cultivation and Anaerobic culturel simultaneously, reduce workload, cost-saving.
Accompanying drawing explanation
Fig. 1 is the culturing bottle structural representation of body fluid culturing bottle of the present invention.
Fig. 2 is the indicator structure schematic diagram of body fluid culturing bottle of the present invention.
Fig. 3 is the schematic diagram of indicator microoraganism growth after body fluid culturing bottle of the present invention inoculation.
Fig. 4 is that after body fluid culturing bottle of the present invention inoculation, indicator microoraganism grows another schematic diagram.
Fig. 5 is that after body fluid culturing bottle of the present invention inoculation, indicator microoraganism grows another schematic diagram.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in further detail:
As shown in Figure 1, 2, body fluid culturing bottle of the present invention, comprise the culturing bottle and telltale that fill substratum, the culturing bottle filling substratum comprises bottle 1, the plug 3 of sealed bottle body and bottle cap 2, the bottle 1 of culturing bottle, built with the liquid nutrient medium and the mixed gas that form microorganism growth prerequisite, is negative pressure gnotobasis in culturing bottle; Telltale is a plastic, transparent reservoir 6 being circumscribed with minute hand 8, has a plastic cover 7 above reservoir, and reservoir Lower Half is the sleeve pipe 9 be connected with culturing bottle, and minute hand 8 is positioned at the central authorities of sleeve pipe 9 and communicates with reservoir 6.
Described culturing bottle bottle 1 is transparent cylindrical bottle, is vial or double-layer plastic bottle, ensures that bottle is transparent and airtight.Described culturing bottle plug 1 is butyl rubber plug.Described culturing bottle bottle cap 2 is aluminium lid, is removed by middle portion during inoculation.
Described reservoir 6 is cylindrical transparent plastic body, and the lower lumen of sleeve pipe 9 and culturing bottle bottle cap 2 size coupling, make minute hand 8 insert in culturing bottle, the bottom of sleeve pipe 9 is stuck in the outside of culturing bottle bottle cap 2, and reservoir is contacted firmly with culturing bottle.
Liquid nutrient medium in culturing bottle contains the nutritive substance promoting aerobic microorganism and anaerobion growth, comprises basal nutrient material tryptic soy broth, brain heart infusion, yeast extract, carbohydrate etc.Comprise to microorganism rise in the microorganism B race of repair, vitamin K race, vitamin-E race one or more.Comprise one or more in the Sodium Citrate of accelerate growth of microorganism, Trisodium Citrate, ironic citrate, S-WAT, magnesium sulfate, arginine, L-cysteine hydrochloride etc.Comprise, reduce other materials interference in sample, one or more in the edetate of accelerate growth of microorganism, para-amino benzoic acid, poly-fennel sodium sulfonate, glucose oxidase, protohemine.
Mixed gas in culturing bottle comprises one or more in oxygen, nitrogen, hydrogen, carbonic acid gas.
Be inoculated into by body fluid sample after in culturing bottle, the minute hand syringe needle of telltale is inserted in liquid nutrient medium by bottle stopper.When microorganism growth produces gas, add the gaseous tension of culturing bottle inside, now gas pressure makes liquid nutrient medium enter into reservoir by syringe needle, if there is nutrient solution in reservoir, has microorganism growth in indicating liquid substratum.When growth certain hour, run out of the oxygen in culturing bottle along with microbial growth aerobic microorganism, then the anaerobion be suppressed can grow.
After there is nutrient solution in reservoir, instruction has microorganism growth, now can carry out the operation that transferred species continues next step.During transferred species in an aseptic environment, the plastic cover of reservoir is opened, draw nutrient solution.This method is easy to operate, and does not affect microorganism continued growth in culturing bottle.
Liquid nutrient medium comprises:
Tryptic soy broth 1-50%, yeast extract 0.1-10%, brain heart infusion 0.1-10%, sucrose 0.1-10%, glucose 0.1-10%, sodium-chlor 0.5-5%, para-amino benzoic acid 0.005-1%, poly-anetholesulfonic acid sodium 0.05-1%, Sodium Citrate 0.25-2.5%, arginine 0.25-2.5%, potassium primary phosphate 0.25-2.5%, VITAMIN 0.00005-1%, surplus is purified water.
Preferably:
Tryptic soy broth 2.5%, yeast extract 0.3%, brain heart infusion 0.1%, sucrose 0.2%, glucose 0.2%, sodium-chlor 0.5%, para-amino benzoic acid 0.005%, poly-anetholesulfonic acid sodium 0.05%, Sodium Citrate 0.25%, arginine 0.25%, potassium primary phosphate 0.25%, VITAMIN 0.00065%, surplus is purified water.
Tryptic soy broth 3%, yeast extract 0.2%, brain heart infusion 0.1%, sucrose 0.25%, glucose 0.15%, sodium-chlor 0.5%, para-amino benzoic acid 0.005%, poly-anetholesulfonic acid sodium 0.05%, Sodium Citrate 0.25%, arginine 0.25%, potassium primary phosphate 0.3%, VITAMIN 0.0005%, surplus is purified water.
Tryptic soy broth 2%, yeast extract 0.4%, brain heart infusion 0.1%, sucrose 0.25%, glucose 0.4%, sodium-chlor 0.5%, para-amino benzoic acid 0.005%, poly-anetholesulfonic acid sodium 0.05%, Sodium Citrate 0.3%, arginine 0.25%, potassium primary phosphate 0.25%, VITAMIN 0.001%, surplus is purified water.
Mixed gas comprises 5% carbonic acid gas, 95% air.
Embodiment 1, by the body fluid sample gathered, by negative pressure in culturing bottle, be injected in culturing bottle, the minute hand of telltale is inserted into liquid nutrient medium inside, reservoir plastic cover is built.When the gas produced during microorganism growth in sample makes culturing bottle internal pressure increase, make nutrient solution enter reservoir by minute hand, when there is nutrient solution in the reservoir of telltale, microorganism growth (as Fig. 3) has been described
Embodiment 2, is injected in culturing bottle by the body fluid sample of collection, and the minute hand of telltale is inserted into liquid nutrient medium inside, and reservoir plastic cover is built.When the gas produced during microorganism growth in sample makes culturing bottle internal pressure increase, nutrient solution is made to enter reservoir by minute hand, if the nutrient solution in the reservoir of telltale is less, illustrate that the microorganism existed in sample belongs to the less Pseudomonas of gas production rate, the substratum (as Fig. 4) of transferred species can be selected according to this.
Embodiment 3, if the nutrient solution in the reservoir of telltale is more, illustrates that the microorganism existed in sample belongs to the more Pseudomonas of gas production rate, can select the substratum (as Fig. 5) of transferred species according to this.
Embodiment 4, if the nutrient solution liquid level rate of growth in telltale stage by stage or long-time continuous increase, not only a kind of microorganism may be there is, transferred species cultivation can be carried out stage by stage, what the later stage increased may be anerobe, need timely transferred species, and carry out improving transferred species success ratio under obligate anaerobic culture condition.
Above-described embodiment is only for illustration of technological thought of the present invention and feature, its object is to enable those skilled in the art understand content of the present invention and implement according to this, only can not limit the scope of the claims of the present invention with the present embodiment, namely the equal change done of all disclosed spirit or modification, still drop in the scope of the claims of the present invention.
Claims (5)
1. a body fluid culturing bottle, it is characterized in that, comprise the culturing bottle and telltale that fill substratum, the culturing bottle filling substratum comprises bottle (1), the plug (3) of sealed bottle body and bottle cap (2), telltale is a plastic, transparent reservoir (6) being circumscribed with minute hand (8), a plastic cover (7) is had above reservoir, reservoir Lower Half is the sleeve pipe (9) be connected with culturing bottle, and minute hand (8) is positioned at the central authorities of sleeve pipe (9) and communicates with reservoir (6).
2. body fluid culturing bottle according to claim 1, is characterized in that, described culturing bottle bottle (1) is transparent cylindrical bottle, is vial or double-layer plastic bottle, ensures that bottle is transparent and airtight.
3. body fluid culturing bottle according to claim 1, is characterized in that, described culturing bottle plug (1) is butyl rubber plug.
4. body fluid culturing bottle according to claim 1, is characterized in that, described culturing bottle bottle cap (2) is aluminium lid, is removed by middle portion during inoculation.
5. body fluid culturing bottle according to claim 1, it is characterized in that, described reservoir (6) is cylindrical transparent plastic body, the lower lumen of sleeve pipe (9) and culturing bottle bottle cap (2) size coupling, minute hand (8) is made to insert in culturing bottle, the bottom of sleeve pipe (9) is stuck in the outside of culturing bottle bottle cap (2), and reservoir is contacted firmly with culturing bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610093544.6A CN105505752A (en) | 2016-02-18 | 2016-02-18 | Body fluid culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610093544.6A CN105505752A (en) | 2016-02-18 | 2016-02-18 | Body fluid culture bottle |
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| Publication Number | Publication Date |
|---|---|
| CN105505752A true CN105505752A (en) | 2016-04-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610093544.6A Pending CN105505752A (en) | 2016-02-18 | 2016-02-18 | Body fluid culture bottle |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5047331A (en) * | 1983-02-04 | 1991-09-10 | Oxoid Limited | Method and device for bacterial testing |
| CN2575675Y (en) * | 2002-08-20 | 2003-09-24 | 李开 | Flashing lightning magnetic field detector |
| CN2883386Y (en) * | 2006-03-24 | 2007-03-28 | 朱核光 | Microbe breathing rate analyzer based on bulbbler counting |
| CN201148437Y (en) * | 2007-12-27 | 2008-11-12 | 徐永涛 | Biphase blood culture apparatus of automatic subcuitivation |
| CN104673662A (en) * | 2013-12-03 | 2015-06-03 | 深圳市艾瑞生物科技有限公司 | Culture bottle and preparation method and application thereof |
| CN204918585U (en) * | 2015-08-04 | 2015-12-30 | 武汉迪艾斯科技有限公司 | Blood sample bacterium detection device |
| CN205443285U (en) * | 2016-02-18 | 2016-08-10 | 允汇科技（天津）有限公司 | Body fluid blake bottle |
-
2016
- 2016-02-18 CN CN201610093544.6A patent/CN105505752A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5047331A (en) * | 1983-02-04 | 1991-09-10 | Oxoid Limited | Method and device for bacterial testing |
| CN2575675Y (en) * | 2002-08-20 | 2003-09-24 | 李开 | Flashing lightning magnetic field detector |
| CN2883386Y (en) * | 2006-03-24 | 2007-03-28 | 朱核光 | Microbe breathing rate analyzer based on bulbbler counting |
| CN201148437Y (en) * | 2007-12-27 | 2008-11-12 | 徐永涛 | Biphase blood culture apparatus of automatic subcuitivation |
| CN104673662A (en) * | 2013-12-03 | 2015-06-03 | 深圳市艾瑞生物科技有限公司 | Culture bottle and preparation method and application thereof |
| CN204918585U (en) * | 2015-08-04 | 2015-12-30 | 武汉迪艾斯科技有限公司 | Blood sample bacterium detection device |
| CN205443285U (en) * | 2016-02-18 | 2016-08-10 | 允汇科技（天津）有限公司 | Body fluid blake bottle |
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Application publication date: 20160420 |