CN2883386Y - Microbe breathing rate analyzer based on bulbbler counting - Google Patents
Microbe breathing rate analyzer based on bulbbler counting Download PDFInfo
- Publication number
- CN2883386Y CN2883386Y CN 200620004632 CN200620004632U CN2883386Y CN 2883386 Y CN2883386 Y CN 2883386Y CN 200620004632 CN200620004632 CN 200620004632 CN 200620004632 U CN200620004632 U CN 200620004632U CN 2883386 Y CN2883386 Y CN 2883386Y
- Authority
- CN
- China
- Prior art keywords
- standpipe
- type passage
- rate determination
- respiratory rate
- outlet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
Abstract
The utility model relates to a microbial respiration speed measuring device based on air bubble counts, on which a microbial culture bottle (1) is arranged; the gas phase of the microbial culture bottle (1) is connected with a U-shaped channel (2); transparent sealed solution (23) is arranged in the U-shaped channel (2); an inlet standpipe (22) of the U-shaped channel (2) serves as an air inlet, an outlet standpipe (21) serves an air outlet; a photo-electric sensing and measuring device (4) is arranged outside the transparent location with sealed solution of the outlet standpipe (21) for measuring air bubbles in the outlet standpipe (21). The utility model has the advantages of wider range of microbial respiration measurement, higher accuracy, stability and easy maintenance for multiple purposes.
Description
Technical field
The utility model relates to a kind of microbial respiratory rate determination device, particularly a kind of microbial respiratory rate determination device based on bubble counting.
Background technology
Existing sophisticated aerobic respiration rate determination all is based in the airtight microorganism culturing bottle, the dissolved oxygen that causes owing to the respiration of microorganism or the variation of pressure.By using dissolved oxygen probe (dissolved oxygen sensor) or pressure probe (pressure transmitter), write down this variation, and reach the purpose of mensuration.The weak point of these methods is: 1 can only measure the sample of aerobic respiration speed in the certain value scope, the sample that exceeds the permissible value scope must dilute just and can carry out, 2 restricted by the precision of transmitter, the precision of measuring is relatively low, 3 owing to transmitter contacts with specimen directly or indirectly, its performance is vulnerable to the influence of sample physico-chemical property, brings difficulty for simultaneously the calibration of transmitter, and 4 transmitters require high aspect maintaining.
Summary of the invention
The utility model has solved existing utilization and has measured the variation of dissolved oxygen or pressure and measure existing technical problem in the device of microbial respiratory speed, and a kind of microbial respiratory rate determination device based on bubble counting is provided.
In order to achieve the above object, the technical scheme that the utility model is taked is: a kind of microbial respiratory rate determination device based on bubble counting, be provided with the microorganism culturing bottle, the gas phase of microorganism culturing bottle is connected with U type passage, the sealing liquid of light-permeable is housed in described U type passage, the inlet standpipe of U type passage is the gas upstream end, the outlet standpipe of U type passage is the gas outlet end, the outside, position light-permeable, that sealing liquid is arranged on described outlet standpipe, installation are used to detect the optoelectronic induction proofing unit of the interior bubble of outlet standpipe by quantity.
Preferred version as above technical scheme: the pipe connecting between two standpipes of U type passage is downward-sloping to the outlet standpipe by the inlet standpipe.
Preferred version as above technical scheme: U type passage or outlet standpipe are to be made by the material of light-permeable.
Preferred version as above technical scheme: described sealing liquid is the stable and difficult evaporable liquid of chemical property.
Preferred version as above technical scheme: described optoelectronic induction proofing unit is provided with luminescent device in described outlet standpipe one side, opposite side at the outlet standpipe relative with this luminescent device is provided with the sensing device that the induction light line strength changes, and the output terminal of sensing device is connected with signal handling equipment, signal record display equipment.
Preferred version as above technical scheme: the light that described luminescent device sends by the outlet standpipe axis, with sensing device over against.
Preferred version as above technical scheme: described optoelectronic induction proofing unit is provided with luminescent device in described outlet standpipe one side, opposite side at the outlet standpipe relative with this luminescent device is provided with the sensing device that the induction light line strength changes, the output terminal of sensing device is connected with signal handling equipment, and the output terminal of signal handling equipment is connected with the signal record display equipment.
Preferred version as above technical scheme: the gas phase of this microorganism culturing bottle is connected with the gas phase of the outlet standpipe of U type passage, and the gas phase of the inlet standpipe of U type passage is connected with the oxygen supply device of oxygen supply pressure-stabilisation.
Preferred version as above technical scheme: oxygen supply device is the source of oxygen that stable-pressure device is set.
Preferred version as above technical scheme: the gas phase of this microorganism culturing bottle is connected with the gas phase of the inlet standpipe of U type passage, and the gas phase of the outlet standpipe of U type passage is connected with atmosphere.
The beneficial effect of the microbial respiratory rate determination device based on bubble counting of the present utility model is: it is big to measure the microbial respiratory speed range, the precision height; And because photoelectric induction device discord sample has any contact, therefore, its maintaining becomes simple, and performance can be kept stable for a long time.The precision of monitoring and sensitivity can be brought up in the scope of single isolated bubbles variation (several milliliter), measured precision height.
The direction of passing through to change U type passage based on the microbial respiratory rate determination device of bubble counting of the present utility model, be directly used in the aerobic respiration speed of wastewater biochemical oxygen requirement (being called for short BOD), active sludge and soil, the microbiological anaerobic factor of created gase, and the mensuration of other relative index and parameter, reach the purpose of a tractor serves several purposes.
Description of drawings
Fig. 1 represents that the utility model embodiment's 1 is used for BOD and aerobic respiration rate determination device and theory structure synoptic diagram;
Fig. 2 represents the structural representation of U type passage among the utility model embodiment 1;
Fig. 3 represents the frame assumption diagram of optoelectronic induction proofing unit among the utility model embodiment 1;
Fig. 4 represents to be used among the utility model embodiment 2 anaerobic gas generation rate determinator and theory structure synoptic diagram.
Embodiment
With reference to accompanying drawing, will be described in detail embodiment of the present utility model.
Embodiment 1
Present embodiment is a kind of microbial respiratory rate determination device based on bubble counting that is used for wastewater biochemical oxygen requirement (being called for short BOD), reaches the aerobic respiration rate determination, and Fig. 1 illustrates its device and theory structure synoptic diagram; This device is provided with U type passage 2, the sealing liquid 23 of light-permeable is housed in U type passage 2, also be provided with microorganism culturing bottle 1, this microorganism culturing bottle 1 built-in test sample (as: wastewater sample that will measure) and microbial strains, the gas phase of this microorganism culturing bottle 1 is connected with the gas phase of the outlet standpipe 21 of U type passage 2, and the gas phase of the inlet standpipe 22 of U type passage 2 is connected with the oxygen supply device 3 of oxygen supply pressure-stabilisation, and outside described outlet standpipe 21 has the position of sealing liquid optoelectronic induction proofing unit 4 is installed.
Particularly, described oxygen supply device 3 comprises source of oxygen 31, the outlet of this source of oxygen 31 is divided into two pipelines, article one, the gas phase with described inlet standpipe 22 is connected, and keeps certain in order to make oxygen pressure, and another pipeline connects pressure equilibrium bottle 32, this pressure equilibrium bottle 32 can be existing, as shown in the figure, oxygen input tube is inserted under the liquid level in the pressure equilibrium bottle 32, and the gas phases in the pressure equilibrium bottle 32 communicate with atmosphere.Certainly, also can keep certain by the oxygen pressure that other structure of voltage regulation provide oxygen supply device.
Described optoelectronic induction proofing unit 4, frame assumption diagram as shown in Figure 3 comprises luminescent device 41, sensing device 42 and signal handling equipment 43, signal record display equipment 44.Wherein, the light that described luminescent device 41 sends can be infrared rays, laser or other monochromes, polychromatic light.This sensing device 2 can be all kinds of photosensors, such as photodiode (model can be HPD731GAW), in this example, photodiode and resistance series connection back power according to normal running conditions, as bubble through out-of-date, bubble changes light, the resistance of photodiode is changed, thereby the electric current through this photodiode and series resistance is changed, by detecting the variation of this resistance both end voltage, the numerical value that is specifically changed, thus can judge the quantity that bubble passes through, can calculate the gas volume that passes through by signal handling equipment 43 again, the result can be outputed on the signal record display equipment 44 at last.Certainly, also can respond to, detect because the variation of bubble by the light that causes, thereby can obtain passing through bubble quantity and bubble by additive method.
In order to detect bubble better by data, thus the best axis by outlet standpipe 21 of the light that luminescent device 41 sends, with sensing device 42 over against.
Pipe connecting 24 between two standpipes of described U type passage 2 is preferably downward-sloping to the outlet standpipe by inlet standpipe 22, and when passing through with assurance gas, under capillary effect, gas can form bubble of uniform size.
Described sealing liquid 23 can be stablized and difficult evaporable liquid for any chemical property.With the precision that guarantees to measure.
Described U type passage 2 materials can comprise all kinds of organic or inorganic glass for the meable material of light, and resin etc. also can be only be made the position of these outlet standpipe 21 installing optoelectronic induction proofing units 4 transparent, allow light to pass through, and are detected.
During work, because the existence of sealing liquid 23 in the U type passage 2, make the U type passage 2 both sides balance that keep-ups pressure, along with the microorganism oxygen absorbed in the microorganism culturing bottle 1 increases, the pressure of microorganism culturing bottle 1 side reduces, pressure less than oxygen supply device 3 sides, so oxygen can enter microorganism culturing bottle 1 one sides through sealing liquid 23 along U type passage 2, in through sealing liquid 23 processes, because both sides pressure reduction is basic identical, thus know from experience formation bubble of uniform size in the capillary effect therapeutic method to keep the adverse qi flowing downward, under the experiment condition of Fig. 1, bubble is to link up according to the order of sequence to pass through U type passage, the path that the light that described luminescent device 41 sends passes through by bubble in the U type channel outlet standpipe 21, and pass on the sensing device 42, when bubble passes through, sensing device 42 just can be sensed the variation of light intensity, thereby records passing through of bubble.Therefore, can measure bubble quantity and bubble,, thereby can directly converse amount of oxygen and the aerobic speed that is consumed in conjunction with the air content of single isolated bubbles by this device.
Embodiment 2
The microbial respiratory rate determination device based on bubble counting of present embodiment, be the mensuration that is used for the anaerobic gas generation rate, this device and theory structure synoptic diagram are as shown in Figure 4, be provided with microorganism culturing bottle 1 (containing specimen and microbial strains), U type passage 2, interior sealing liquid 23 with light-permeable, optoelectronic induction proofing unit 4.The gas phase of this microorganism culturing bottle 1 is connected with the gas phase of the inlet standpipe 22 of U type passage 2, and the gas phase of the outlet standpipe 21 of U type passage 2 is connected with atmosphere, and outside described outlet standpipe 21 has the position of sealing liquid 23, optoelectronic induction proofing unit 4 is installed, other conditions are identical with embodiment 1.
During work, because the existence of sealing liquid 23 in the U type passage 2, make the U type passage 2 both sides balance that keep-ups pressure, the increase of the gas that is produced along with the microbiological anaerobic metabolism in the microorganism culturing bottle 1, the pressure of microorganism culturing bottle 1 side increases, when greater than the pressure of outlet standpipe 21 sides, gas can be discharged atmosphere through sealing liquid 23 along U type passage 2, in through sealing liquid 23 processes, because both sides pressure reduction is basic identical, and because pipe connecting 24 is downward-sloping to the outlet standpipe by inlet standpipe 22, so know from experience formation bubble of uniform size in the capillary effect therapeutic method to keep the adverse qi flowing downward, under the experiment condition of Fig. 4, bubble is to link up according to the order of sequence to pass through U type passage, the path that the light that described luminescent device 41 sends passes through by bubbles in the U type channel outlet standpipe 21, and pass on the sensing device 42, when bubble passed through, sensing device 42 just can be sensed the variation of light intensity, thereby records passing through of bubble.Therefore, can measure bubble quantity and bubble,, thereby can directly converse gas volume and the gas production rate that is produced in conjunction with the air content of single isolated bubbles by this device.
Embodiment 3
Other conditions are identical with embodiment 1 or embodiment 2, just in order to satisfy the needs of measuring a plurality of samples simultaneously, above-mentioned a plurality of microorganism culturing bottle and coupled U type passage are set, outside the outlet standpipe of each U type passage luminescent device, sensing device are set, described a plurality of sensing device induced signal output parts are connected with one or more multi channel signals treatment facilities and signal record display equipment.Can utilize above a plurality of microorganism culturing bottles arranged side by side, U type passage, luminescent device, sensing device, gather the data of many group samples, again a plurality of sensing device induced signals are passed to one or more multi channel signals treatment facilities and signal record display equipment, can reduce cost like this, increase work efficiency.This multi channel signals treatment facility is meant the equipment that can handle a plurality of input signals simultaneously, and handling principle is identical with the signal handling equipment of a passage.
Claims (10)
1, a kind of microbial respiratory rate determination device based on bubble counting, be provided with microorganism culturing bottle (1), it is characterized in that, the gas phase of microorganism culturing bottle (1) is connected with U type passage (2), the sealing liquid (23) of light-permeable is housed in described U type passage (2), the inlet standpipe (22) of U type passage (2) is the gas upstream end, the outlet standpipe (21) of U type passage (2) is the gas outlet end, go up light-permeable at described outlet standpipe (21), the outside, position that sealing liquid is arranged, installation are used for detecting the optoelectronic induction proofing unit (4) of the interior bubble of outlet standpipe (21) by quantity.
2, the microbial respiratory rate determination device based on bubble counting according to claim 1 is characterized in that, the pipe connecting (24) between two standpipes of U type passage (2) is downward-sloping to outlet standpipe (21) by inlet standpipe (22).
3, the microbial respiratory rate determination device based on bubble counting according to claim 2 is characterized in that, U type passage (2) or outlet standpipe (21) are to be made by the material of light-permeable.
4, the microbial respiratory rate determination device based on bubble counting according to claim 2 is characterized in that, described sealing liquid (23) is stable and difficult evaporable liquid for chemical property.
5, the microbial respiratory rate determination device based on bubble counting according to claim 2, it is characterized in that, described optoelectronic induction proofing unit (4) is provided with luminescent device (41) in described outlet standpipe (21) one sides, opposite side at the outlet standpipe (21) relative with this luminescent device (41) is provided with the sensing device (42) that the induction light line strength changes, the output terminal of sensing device (42) is connected with signal handling equipment (43), and the output terminal of signal handling equipment (43) is connected with signal record display equipment (44).
6, the microbial respiratory rate determination device based on bubble counting according to claim 5, it is characterized in that, the light that described luminescent device (41) sends by the outlet standpipe (21) axis, with sensing device (42) over against, described sensing device (42) is a photosensor.
7, the microbial respiratory rate determination device based on bubble counting according to claim 5, it is characterized in that, above-mentioned a plurality of microorganism culturing bottle and coupled U type passage are set, outside the outlet standpipe of each U type passage luminescent device, sensing device are set, described a plurality of sensing device induced signal output parts are connected with one or more multi channel signals treatment facilities and signal record display equipment.
8, according to the arbitrary described microbial respiratory rate determination device of claim 1 to 7 based on bubble counting, it is characterized in that, the gas phase of microorganism culturing bottle (1) is connected with the gas phase of the outlet standpipe (21) of U type passage (2), and the gas phase of the inlet standpipe (22) of U type passage (2) is connected with the oxygen supply device (3) of oxygen supply pressure-stabilisation.
9, the microbial respiratory rate determination device based on bubble counting according to claim 8 is characterized in that, oxygen supply device (3) is the source of oxygen (31) that stable-pressure device is set.
10, according to the arbitrary described microbial respiratory rate determination device of claim 1 to 7 based on bubble counting, it is characterized in that, the gas phase of this microorganism culturing bottle (1) is connected with the gas phase of the inlet standpipe (22) of U type passage (2), and the gas phase of the outlet standpipe (21) of U type passage (2) is connected with atmosphere.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620004632 CN2883386Y (en) | 2006-03-24 | 2006-03-24 | Microbe breathing rate analyzer based on bulbbler counting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620004632 CN2883386Y (en) | 2006-03-24 | 2006-03-24 | Microbe breathing rate analyzer based on bulbbler counting |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2883386Y true CN2883386Y (en) | 2007-03-28 |
Family
ID=37956448
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200620004632 Expired - Fee Related CN2883386Y (en) | 2006-03-24 | 2006-03-24 | Microbe breathing rate analyzer based on bulbbler counting |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2883386Y (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101554327B (en) * | 2009-05-04 | 2011-06-15 | 哈尔滨工业大学 | Method for measuring breathing rate of aquatic oligochaeta worm |
| CN105505752A (en) * | 2016-02-18 | 2016-04-20 | 允汇科技（天津）有限公司 | Body fluid culture bottle |
| CN107063922A (en) * | 2017-02-27 | 2017-08-18 | 湖北洛克泰克仪器有限公司 | A kind of full-automatic Biochemical Methane Potential test system |
| CN107631751A (en) * | 2017-09-08 | 2018-01-26 | 沈阳农业大学 | Measuring gas flow rate system and assay method based on connecting and disconnecting of the circuit |
-
2006
- 2006-03-24 CN CN 200620004632 patent/CN2883386Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101554327B (en) * | 2009-05-04 | 2011-06-15 | 哈尔滨工业大学 | Method for measuring breathing rate of aquatic oligochaeta worm |
| CN105505752A (en) * | 2016-02-18 | 2016-04-20 | 允汇科技（天津）有限公司 | Body fluid culture bottle |
| CN107063922A (en) * | 2017-02-27 | 2017-08-18 | 湖北洛克泰克仪器有限公司 | A kind of full-automatic Biochemical Methane Potential test system |
| CN107631751A (en) * | 2017-09-08 | 2018-01-26 | 沈阳农业大学 | Measuring gas flow rate system and assay method based on connecting and disconnecting of the circuit |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203324220U (en) | Multi-component gas monitoring device | |
| CN203443606U (en) | Indoor pollution gas concentration monitoring device | |
| CN101692049B (en) | Control system used for COD on-line automatic monitor | |
| CN2883386Y (en) | Microbe breathing rate analyzer based on bulbbler counting | |
| CN105067596A (en) | Ozone detection apparatus and detection method thereof | |
| CN103645290A (en) | Water quality online detector | |
| CN108956927A (en) | A kind of high-precision dissolved oxygen detection device | |
| CN203376015U (en) | Accurate optical quantitative metering device | |
| CN204945050U (en) | Ozone-detecting element | |
| CN104977420B (en) | A kind of water quality analytical system | |
| CN202710577U (en) | Peroxide online detection device | |
| CN201697681U (en) | Light control metering device | |
| CN104007054B (en) | Device and method for detecting cell concentration on line in biological fermentation process | |
| CN111537448A (en) | Adjustable range double-light-source water quality COD (chemical oxygen demand) detection sensor | |
| CN201780286U (en) | Light-operated metering device | |
| CN201440122U (en) | Laser turbidity meter | |
| CN105116031A (en) | Online biochemical oxygen demand detection method and online biochemical oxygen demand detection device | |
| CN104807864A (en) | Digital electrode for measuring content of perfluorocapylic acid and measuring apparatus | |
| CN204855441U (en) | Portable biochemical oxygen demand spot test appearance of AC/DC | |
| CN201622258U (en) | Automatic oil gas concentration detector | |
| CN213580647U (en) | Adjustable range double-light-source water quality COD (chemical oxygen demand) detection sensor | |
| CN210953822U (en) | Analysis system and device for rapidly measuring nitrogen oxides in ambient air | |
| CN211179536U (en) | ATP on-line measuring instrument | |
| CN2552005Y (en) | COD (chemical oxygen demand) all quantity on-line monitor | |
| CN201508491U (en) | Comprehensive managing and alarming system for detecting hospital environment automatically |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070328 |