CN201148437Y

CN201148437Y - Biphase blood culture apparatus of automatic subcuitivation

Info

Publication number: CN201148437Y
Application number: CNU200720188673XU
Authority: CN
Inventors: 徐永涛
Original assignee: Individual
Current assignee: Individual
Priority date: 2007-12-27
Filing date: 2007-12-27
Publication date: 2008-11-12
Anticipated expiration: 2017-12-27

Abstract

The utility model relates to a double-phase blood culture device that can automatically change the phase. The blood culture device comprises a liquid-phase medium part, an indication and solid-state medium part, and a liquid guide component. The liquid-phase medium part is a blood culture bottle filled with the liquid-phase medium; the inside of the bottle stopper of the blood culture bottle is connected with a catheter; the catheter extends into the bottle and at least reach the liquid level of the liquid-phase medium. The indication and solid-state medium part is a container filled with a solid-state medium slide; the cover of the container is provided with a liquid feeding channel; the inner end of the liquid feeding channel is connected with the solid-state medium slide; two surfaces or one surface of the solid-state medium slide is provided with the solid-state medium. The liquid guide component comprises a hose and a pinhead; the pinhead is connected with one end of the hose and is inserted into the bottle stopper of the blood culture bottle to enter the catheter; the other end of the hose is connected with the outer end of the liquid feeding channel of the container of the indication and solid-state medium part; the liquid-phase medium part is connected with the solid-phase medium part in parallel through the liquid guide component, and is then arranged in a seat support. The device has the advantages of simple structure, automatic phase change, simultaneous preparation of the liquid phase and the solid phase, integral functions, high sensitivity of indication, simple operation and so on.

Description

A kind of dual-phase blood culture apparatus of automatic transferred species

Technical field

The utility model relates to a kind of blood cultivation device that is used for blood cultivation.

Background technology

At present, using more general blood cultivation device clinically is the Singal blood culture system of Britain OXOID company.It is a tubule blood cultivation bottle with indicating unit, the blood cultivation bottle is connected up and down by injection needles with indicating unit, needle tip inserts certain position under bottle liquid level of interior nutrient chemical base, when producing gas in the bacterial growth in the blood cultivation bottle air pressure in the bottle is raise gradually, liquid in the bottle is depressed in the tubule as indicating unit by injection needles, makes the positive prompting that obtains that naked eyes observe at an easy rate.Its advantage is simple in structure, and is simple to operate, safeguards easily, if there is not positive prompting, then need not to remake further transferred species and handles, and it is few therefore to receive the external interference factor.Its shortcoming is: because indicating unit is last, action of gravity shows that its susceptibility weakens; Do not have the transferred species device,, not only increase workload but also expend time in if positive prompting still needs the professional and technical personnel to do further transferred species and separates.

Summary of the invention

The purpose of this utility model is the defective at the prior art existence, a kind of dual-phase blood culture apparatus of automatic transferred species is provided, it has under the prerequisite of simple structure in reservation, improve the susceptibility of indicating section on the one hand, increase the transferred species separate part on the other hand, make its more perfect functions, and improve the pollution capacity of avoiding of device.

The technical solution of the utility model is as follows:

A kind of whole blood culture apparatus of automatic transferred species is made up of liquid phase culture medium part, indication and solid phase substratum part and drain spare, it is characterized in that:

Described liquid phase culture medium part is a blood cultivation bottle that liquid phase medium is housed, and is connected with a conduit in the bottle stopper of blood cultivation bottle, and conduit extends under the interior liquid phase medium liquid level of insertion bottle;

Partly for the container of solid phase substratum slide glass is housed, its container cover is provided with feed pathway for described indication and solid phase substratum, the inner solid phase substratum slide glass that connects of feed pathway, and two-sided or single face load has the solid phase substratum on the solid phase substratum slide glass;

Described drain spare comprises a flexible pipe and a syringe needle, syringe needle is connected flexible pipe one end, and the bottle stopper of insertion blood cultivation bottle, enter in the conduit, the other end of flexible pipe is connected with the container feed pathway outer end of indication and solid phase substratum part, liquid phase culture medium part and indication and solid phase substratum part are connected in parallel by drain spare, are contained in the mounting.

Use the device of said structure, when having bacterial growth to produce gas in the liquid phase medium, air pressure in the device raises, liquid is pressed in indication in parallel with it and the solid phase substratum part, is convenient to observe, and convenient automatic transferred species is separated, save time, simplify the operation, can one realize that cultivation, indication separate perfect in shape and function with transferred species.Simultaneously, adopt the form of two portions parallel connection, can weaken the influence of action of gravity, improve display sensitivity its susceptibility.

For liquid import indication and solid phase substratum part unblocked, the coupling end of the feed pathway on solid phase substratum slide glass and the container cover is designed to insert or sleeve type, and is provided with the diversion trench of downward connection at coupling end outer wall or inwall.In addition, the solid phase substratum stably is set on the solid phase substratum slide glass for convenience, difficult drop-off, the main body section of solid phase substratum slide glass is designed to the U-lag shape, and further be provided with the localized raised line of solid phase substratum in the U-lag baseplane, U-lag both sides and lower end are provided with inside draw-in groove or latch.Importing liquid for convenience evenly flows on the solid phase substratum, be provided with the flow-guiding structure that is communicated with coupling end and main body with the transition portion of coupling end above solid phase substratum slide glass main body, this flow-guiding structure is diversion trench or the irregular curb that forms between the coarse particles of uneven distribution.

In addition, not contaminated for fear of the syringe needle of drain spare, its syringe needle is fixed in the sheath, when not in use, avoid syringe needle and extraneous contacting, sheath is placed in blood cultivation bottle bottleneck.

Also have, high-temperature high-voltage sterilizing sterilizing for convenience of culturing bottle, outer wall in the neck Lower Half of the bottle stopper of blood cultivation bottle is provided with air channel longitudinally, and designed a circle latch at the outer wall of neck Lower Half, it is in when sterilization partly clogs the bottleneck state, prevent to come off, be convenient to sterilization in the bottle again.

Therefore, by above-mentioned improvement, this device has simple in structure, transferred species automatically, and the liquid phase solid phase possesses simultaneously, perfect in shape and function, display sensitivity height, the tangible advantage of grade easy and simple to handle.

Description of drawings

Fig. 1 is the structural representation of this device;

Fig. 2 is the vertical view of Fig. 1;

Fig. 3 A is the structural representation of solid phase substratum slide glass;

Fig. 3 B is the B-B sectional view of Fig. 3 A;

Fig. 3 C is the A-A sectional view of Fig. 3 A;

Fig. 4 A is the structural representation of solid phase substratum slide glass;

Fig. 4 B is the B-B sectional view of Fig. 4 A;

Fig. 4 C is the A-A sectional view of Fig. 4 A;

Fig. 5 A is the structural representation of the bottle stopper of culturing bottle;

Fig. 5 B is the A-A sectional view of Fig. 5 A.

Embodiment

Referring to Fig. 1 and Fig. 2, this blood cultivation device is made up of three parts:

Liquid phase culture medium part 1 also is the blood cultivation bottle, in the bottle liquid phase medium is housed;

Indication and solid phase substratum part 3: positive when cultivating, also be that liquid phase culture medium part produces gas when inoculation is gone into blood preparation and turned out bacterium, its pressure is pressed into the mixing liquid of liquid nutrient medium and sample in indication and the solid phase substratum part, indication is cultivated positive, this mixing liquid (bacterium liquid) is flowed through and is fixed in nutrient agar surface on the slide glass simultaneously, thereby nutrient agar is reached the purpose of automatic transferred species bacterium by bacterium on the automatic vaccination.

Drain spare 2, with above two portions parallel connection, and transporting liquid.

Below the concrete structure of each several part is elaborated:

Liquid phase culture medium part 1: described liquid phase culture medium part is a blood cultivation bottle 11 that liquid phase medium 12 is housed, and is connected with a conduit 13 in the bottle stopper 14 of blood cultivation bottle, and conduit 13 extends in the insertion bottle at least to the liquid phase medium liquid level position.In use, conduit can be delivered to indication and solid phase substratum part by drain spare with bacterium liquid.

Indication and solid phase substratum part 3: for the transparent vessel 32 of solid phase substratum slide glass is housed, be used to hold the liquid (bacterium liquid) that is imported on the one hand, be used to hold solid phase substratum and slide glass 32 thereof on the one hand, because transparent and be convenient to observe.Its container cover 33 is provided with feed pathway 34, and the feed pathway 34 inner solid phase substratum slide glasses 32 that connect are beneficial to the liquid of drain spare 2 guiding is imported in the container.Two-sided or single face load has the solid phase substratum on the solid phase substratum slide glass 32.

Drain spare 2: comprise a flexible pipe 21, a syringe needle 22 and a sleeve 23, syringe needle 22 is connected flexible pipe 21 1 ends, and be fixed in the sleeve 23, when cover upper bush 23 on the bottleneck of culturing bottle, the needle point of syringe needle 22 can puncture bottle stopper 14, and inserts in the bottle inner catheter, makes bottle interior and the extraneous purpose that communicates to reach, and sleeve 23 can protect syringe needle not to be subjected to outside contamination simultaneously, and way one is can be with needle guard on the pinhead sleeve; The 2nd, available film or lid, stopper are closed in syringe needle in the sleeve in the sleeve open end.The other end of flexible pipe 21 is connected with container feed pathway 34 outer ends of indication and solid phase substratum part.

Sample is hatched when cultivating in inoculation, and liquid phase culture medium part 1 and indication and solid phase substratum part 3 are connected in parallel by drain spare 2, are contained in the mounting 4.

The structure of solid phase substratum slide glass 32 has following several form:

Referring to Fig. 3 A, Fig. 3 B and Fig. 3 C, the coupling end 321 that solid phase substratum slide glass 32 is connected with feed pathway 34 on the container cover 33 adopts insert or sleeve types, and be provided with the diversion trench 322 of downward connection at coupling end 321 outer walls or inwall, but main body 324 cross sections of solid phase substratum slide glass are the U-lag shape of load solid phase substratum.

Referring to Fig. 4 A, Fig. 4 B and Fig. 4 C, the top of solid phase substratum slide glass main body 324 also can be provided with the flow-guiding structure that is communicated with coupling end and main body with the transition portion 323 of coupling end 321, this flow-guiding structure can be the irregular curb that forms between the coarse particles 327 of uneven distribution, it also can be the diversion trench 329 among Fig. 4 A, be beneficial to make next liquid (bacterium liquid) dispersion train of guiding through the solid phase substratum, or directly form some little teeth groove in solid phase substratum upper end.

On the U-lag baseplane of solid phase substratum slide glass main body 324, be provided with the localized raised line 325 of solid phase substratum, be formed with inside draw-in groove 326 in both sides and lower end, or latch 328 as showing among Fig. 4 A, Fig. 4 B, Fig. 4 C, be convenient to when pouring into the solid phase substratum, substratum flows into wherein, after treating the cooling of solid phase substratum, substratum is firmly secured on the slide glass.Prevent that the liquid (bacterium liquid) that the solid phase substratum is directed into from washing away or soaking and coming off.

The structure of the bottle stopper 14 of blood cultivation bottle is referring to Fig. 5 A and Fig. 5 B, outer wall in its neck Lower Half is distributed with 4 air channels 141 longitudinally, and be that stopper has designed a circle latch 142 when partly filling in state, when producing sterilization, can stably be in half plug state to keep it, be beneficial to the balance of bottle external and internal pressure and reach the purpose of sterilization.

Claims

1, a kind of dual-phase blood culture apparatus of automatic transferred species is made up of liquid phase culture medium part, indication and solid phase substratum part and drain spare, it is characterized in that:

Described liquid phase culture medium part is a blood cultivation bottle that liquid phase medium is housed, and is connected with a conduit in the bottle stopper of blood cultivation bottle, and conduit extends in the insertion bottle at least to the liquid phase medium liquid level position;

2, the dual-phase blood culture apparatus of automatic transferred species according to claim 1, it is characterized in that: the coupling end of the feed pathway on described solid phase substratum slide glass and the container cover adopts insert or sleeve type, and is provided with the diversion trench of downward connection at coupling end outer wall or inwall; But the main body section of solid phase substratum slide glass is the U-lag shape of appendix solid phase substratum, and the top of solid phase substratum slide glass main body is provided with the flow-guiding structure that is communicated with coupling end and main body with the transition portion of coupling end.

3, the dual-phase blood culture apparatus of automatic transferred species according to claim 2 is characterized in that: the flow-guiding structure of described transition portion is diversion trench or the irregular curb that forms between the coarse particles of uneven distribution.

4, the dual-phase blood culture apparatus of automatic transferred species according to claim 2, it is characterized in that: the U-lag baseplane of described solid phase substratum slide glass main part is provided with the localized raised line of solid phase substratum, and there are inside draw-in groove or latch in U-lag both sides and lower end.

5, the dual-phase blood culture apparatus of automatic transferred species according to claim 1 and 2 is characterized in that: the syringe needle of described drain spare is fixed in the sheath, and sheath is placed in blood cultivation bottle bottleneck.

6, the dual-phase blood culture apparatus of automatic transferred species according to claim 1 and 2 is characterized in that: the outer wall of the neck Lower Half of the bottle stopper of described blood cultivation bottle is distributed with air channel longitudinally, and has designed a circle latch at the outer wall of neck Lower Half.