CN216585025U - Wide-mouth culture bottle for fat cells - Google Patents

Wide-mouth culture bottle for fat cells Download PDF

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Publication number
CN216585025U
CN216585025U CN202122826622.8U CN202122826622U CN216585025U CN 216585025 U CN216585025 U CN 216585025U CN 202122826622 U CN202122826622 U CN 202122826622U CN 216585025 U CN216585025 U CN 216585025U
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China
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wide
waste liquid
mouth
silica gel
sealing
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CN202122826622.8U
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Chinese (zh)
Inventor
张晓腾
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Shanxi Boded Biotechnology Co ltd
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Shanxi Boded Biotechnology Co ltd
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Priority to CN202122826622.8U priority Critical patent/CN216585025U/en
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Abstract

The utility model belongs to the technical field of medical instruments and discloses a wide-mouth culture bottle for adipocytes, which comprises a wide-mouth bottle body, wherein a sealing cover is arranged at the bottle mouth of the wide-mouth bottle body, a conveying pipe, a waste liquid discharge pipe and air holes are formed in the sealing cover, the conveying pipe and the waste liquid discharge pipe penetrate through the sealing cover and extend into the wide-mouth bottle body, a biological membrane is arranged in the wide-mouth bottle body and divides the wide-mouth bottle body into an upper waste liquid exchange area and a lower cell culture area, the lower port of the conveying pipe is located in the cell culture area, the lower port of the waste liquid discharge pipe is located in the waste liquid exchange area, the air holes are communicated with the inside and the outside air of the wide-mouth bottle body, and sterile filter elements are filled in the air holes. The utility model can ensure the air tightness of the fat cells in the culture process and prevent air from polluting the fat cells; when the fat cells are sampled, the fat cells can be cleaned, and the cleaned fat cells are easier to observe.

Description

Wide-mouth culture bottle for fat cells
Technical Field
The utility model belongs to the technical field of medical instruments, and particularly relates to a wide-mouth culture bottle for adipocytes.
Background
The autologous fat transplantation operation is to suck redundant subcutaneous fat cells from certain parts of a human body, then purify the sucked mixture, inject medicine to obtain composite fat particles, and transplant the complete particle fat cells to the parts needing fat filling by the self in an injection mode. With the development of medical beauty technology, more and more people select autologous fat transplantation operation, fat cells are required to be placed into a specific container for culture and purification treatment after being extracted, and medicines are injected to obtain composite fat particles, and the complete particle fat is selected and transplanted to the part of the people needing fat filling in an injection mode.
At present, fat extraction and injection in the fat transplantation operation are finished at one time, and extracted fat can be transplanted after being treated to remove blood, grease and the like, so that the waiting time of an operator is increased. The adipocytes for transplantation can be cultured and then injected to the operator at a specific time, and the cultured adipocytes cannot cause secondary contamination.
SUMMERY OF THE UTILITY MODEL
In view of the above, the present invention provides a wide-mouth culture flask for adipocytes, which solves the problem of the prior art that the culture and washing of adipocytes are performed separately.
In order to achieve the purpose, the utility model provides the following technical scheme:
the utility model provides an adipocyte's wide-mouth blake bottle, includes the wide-mouth bottle, the bottleneck department of wide-mouth bottle is equipped with sealed lid, be equipped with conveyer pipe, waste liquid calandria and bleeder vent on the sealed lid, the conveyer pipe with the waste liquid calandria all passes sealed lid stretches into in the wide-mouth bottle, be equipped with the biomembrane in the wide-mouth bottle, the biomembrane divide into the waste liquid exchange area of top and the cell culture area of below with the wide-mouth bottle, the lower port of conveyer pipe is located the cell culture area, the lower port of waste liquid calandria is located the waste liquid exchange area, inside and the outside air of bleeder vent intercommunication wide-mouth bottle, the downthehole packing of bleeder vent has aseptic filter core.
As the preferred scheme of above technical scheme, the upper end of conveyer pipe is equipped with first sealing silica gel cover, and the syringe needle can pass first sealing silica gel cover and carry cell culture solution or normal saline etc. has guaranteed certain leakproofness in the wide-necked bottle simultaneously.
As the preferred scheme of above technical scheme, the upper end of waste liquid calandria is equipped with second sealing silica gel cover, and the syringe needle can pass second sealing silica gel cover and extract the waste liquid of wasing the cell, has guaranteed certain leakproofness in the wide-necked bottle simultaneously.
As a preferred embodiment of the above technical solution, the sealing cover is provided with a first groove for accommodating the first silica gel sleeve, the first silica gel sleeve is matched with the first groove in shape and accommodated in the first groove, and the sealing cover is flush with the upper surface of the first silica gel sleeve, so that the position of the first silica gel sleeve can be easily found on the sealing cover.
As a preferred embodiment of the above technical solution, the sealing cover is provided with a second groove for accommodating the second silica gel sleeve, the second silica gel sleeve is matched with the second groove in shape and accommodated in the second groove, and the sealing cover is flush with the upper surface of the second silica gel sleeve, so that the position of the second silica gel sleeve can be easily found on the sealing cover.
As the preferable scheme of the technical scheme, the sealing ring is arranged between the sealing cover and the wide-mouth bottle body, so that certain sealing performance in the wide-mouth bottle body is guaranteed.
When the device is used, the fat cells are injected into a cell culture area through a delivery pipe by using an injector, cell culture solution is periodically added through the delivery pipe, waste liquid of cell metabolism is sucked out through a waste liquid discharge pipe, and the fat cells cannot be sucked out when the waste liquid is sucked out due to the fact that the device is provided with a biological membrane; when the fat cells need to be sampled and observed, enough physiological saline is injected through the conveying pipe to clean the fat cells, then the cleaned waste liquid is sucked out from the waste liquid drainage pipe, the fat cells to be observed are taken out through the conveying pipe, and the cleaned fat cells are easier to observe.
The wide-mouth culture bottle for the adipocytes can ensure the air tightness of the adipocytes in the culture process and prevent air from polluting the adipocytes; when the fat cells are sampled, sufficient physiological saline is injected through the conveying pipe to clean the fat cells, the cleaned waste liquid is sucked out from the waste liquid discharge pipe, the fat cells to be observed are taken out through the conveying pipe, and the cleaned fat cells are easier to observe.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Detailed Description
For the purpose of enhancing the understanding of the present invention, the present invention will be further described in detail with reference to the following examples and the accompanying drawings, which are only used for explaining the present invention and are not to be construed as limiting the scope of the present invention.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present.
For convenience in reading, the terms "upper," "lower," "left," and "right" are therefore used in reference to the figures, where the reference relative positions of various elements are intended to be indicated, and not to limit the application.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the utility model is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model.
Examples
Referring to fig. 1, a wide-mouth culture bottle of adipocyte, including wide-mouth bottle 1, the bottleneck department of wide-mouth bottle 1 is equipped with sealed lid 2, is equipped with sealing washer 3 between sealed lid 2 and the wide-mouth bottle 1, has guaranteed the leakproofness of wide-mouth bottle 1. The sealing cover 2 is provided with a conveying pipe 4, a waste liquid calandria 5 and an air vent 6, and the conveying pipe 4 and the waste liquid calandria 5 penetrate through the sealing cover 2 and go deep into the wide-mouth bottle body 1. Be equipped with biomembrane 7 in the wide-mouth bottle 1, biomembrane 7 divide into the waste liquid exchange area 11 of top and the cell culture district 12 of below with wide-mouth bottle 1, and the lower port of conveyer pipe 4 is located cell culture district 12, and the lower port of waste liquid calandria 5 is located waste liquid exchange area 11, and 6 inside and the outside air of intercommunication wide-mouth bottle 1 of bleeder vent, the intussuseption of bleeder vent 6 are filled with aseptic filter core.
The upper end of conveyer pipe 4 is equipped with first sealing silica gel cover 41, and the syringe needle can pass first sealing silica gel cover 41 and carry cell culture solution or normal saline etc. has guaranteed certain leakproofness in the wide-mouth bottle 1 simultaneously.
The upper end of the waste liquid calandria 5 is provided with a second sealing silica gel sleeve 51, and the syringe needle can penetrate through the second sealing silica gel sleeve 51 to extract the waste liquid for cleaning cells, thereby ensuring certain tightness in the wide-mouth bottle body 1.
The sealing cover 2 is provided with a first groove for accommodating the first silica gel sleeve 41, the shape of the first silica gel sleeve 41 is matched with that of the first groove and is accommodated in the first groove, and the upper surfaces of the sealing cover 2 and the first silica gel sleeve 41 are flush, so that the position of the first silica gel sleeve 41 can be easily found on the sealing cover.
The sealing cover 2 is provided with a second groove for accommodating the second silica gel sleeve 51, the shape of the second silica gel sleeve 51 is matched with that of the second groove and is accommodated in the second groove, and the upper surfaces of the sealing cover 2 and the second silica gel sleeve 51 are flush, so that the position of the second silica gel sleeve can be easily found on the sealing cover.
When in use, the fat cells are injected into the cell culture area 12 through the delivery pipe 4 by using an injector, the cell culture solution is periodically added through the delivery pipe 4, the waste liquid of cell metabolism is sucked out through the waste liquid discharge pipe 5, and the fat cells cannot be sucked out when the waste liquid is sucked out due to the biomembrane; when the fat cells need to be sampled and observed, enough physiological saline is injected through the conveying pipe 4 to flush the fat cells, then the flushed waste liquid is sucked out from the waste liquid discharge pipe 5, the fat cells to be observed are taken out through the conveying pipe 4, and the washed fat cells are easier to observe.
The wide-mouth culture bottle for the adipocytes can ensure the air tightness of the adipocytes in the culture process and prevent air from polluting the adipocytes; when the fat cells are sampled, sufficient physiological saline is injected through the conveying pipe to clean the fat cells, the cleaned waste liquid is sucked out from the waste liquid discharge pipe, the fat cells to be observed are taken out through the conveying pipe, and the cleaned fat cells are easier to observe.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the utility model, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (6)

1. The utility model provides an adipocyte's wide-mouth blake bottle, includes the wide-mouth bottle, the bottleneck department of wide-mouth bottle is equipped with sealed lid, its characterized in that: be equipped with conveyer pipe, waste liquid calandria and bleeder vent on the sealed lid, the conveyer pipe with the waste liquid calandria all passes sealed lid stretches into in the wide-necked bottle, be equipped with the biomembrane in the wide-necked bottle, the biomembrane divide into the waste liquid exchange area of top and the cell culture district of below with the wide-necked bottle, the lower port of conveyer pipe is located the cell culture district, the lower port of waste liquid calandria is located the waste liquid exchange area, inside and the outside air of bleeder vent intercommunication wide-necked bottle, the downthehole aseptic filter core that is filled of bleeder vent.
2. The wide-mouth culture flask for the adipocytes of claim 1, wherein: the upper end of conveyer pipe is equipped with first sealing silica gel cover.
3. An adipocyte jar as claimed in claim 1 or 2 wherein: and a second sealing silica gel sleeve is arranged at the upper end of the waste liquid discharge pipe.
4. The wide-mouth culture flask for the adipocytes of claim 2, wherein: the sealing cover is provided with a first groove for accommodating the first sealing silica gel sleeve, the first sealing silica gel sleeve is matched with the first groove in shape and accommodated in the first groove, and the sealing cover is flush with the upper surface of the first sealing silica gel sleeve.
5. The wide-mouth culture flask for the adipocytes of claim 3, wherein: the sealing cover is provided with a second groove for accommodating the second sealing silica gel sleeve, the shape of the second sealing silica gel sleeve is matched with that of the second groove and is accommodated in the second groove, and the upper surfaces of the sealing cover and the second sealing silica gel sleeve are flush.
6. The wide-mouth culture flask for the adipocytes of claim 1, wherein: and a sealing ring is arranged between the sealing cover and the wide-mouth bottle body.
CN202122826622.8U 2021-11-17 2021-11-17 Wide-mouth culture bottle for fat cells Active CN216585025U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202122826622.8U CN216585025U (en) 2021-11-17 2021-11-17 Wide-mouth culture bottle for fat cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202122826622.8U CN216585025U (en) 2021-11-17 2021-11-17 Wide-mouth culture bottle for fat cells

Publications (1)

Publication Number Publication Date
CN216585025U true CN216585025U (en) 2022-05-24

Family

ID=81646532

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202122826622.8U Active CN216585025U (en) 2021-11-17 2021-11-17 Wide-mouth culture bottle for fat cells

Country Status (1)

Country Link
CN (1) CN216585025U (en)

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