CN218174984U

CN218174984U - Cell culture dish for research on apoptosis of microglia

Info

Publication number: CN218174984U
Application number: CN202122766905.8U
Authority: CN
Inventors: 买晨迪
Original assignee: Sanquan College of Xinxiang Medical University
Current assignee: Sanquan College of Xinxiang Medical University
Priority date: 2021-11-10
Filing date: 2021-11-10
Publication date: 2022-12-30
Anticipated expiration: 2031-11-10

Abstract

The utility model discloses a cell culture dish for research of little glial cell burnt death belongs to medical treatment technical field. The boss is installed to culture dish body outer wall, sealed lid outside cover has the rubber sleeve, increase the leakproofness between sealed lid and the culture dish body through the rubber sleeve, the slipmat is installed to culture dish body bottom, guarantee that the culture dish body is stable in the experiment, after the cell loading is accomplished, the culture dish body is put into in the base, the culture dish body is placed more stably, in order to prevent to open sealed lid at every turn, lead to this internal cell of culture dish to receive the pollution, install a plurality of injection syringes on the sealed lid, be provided with the ring channel between aseptic tube and the pipe cover, when the state of stewing, the sealing washer and the ring channel joint of keeping off the installation of lid bottom, guarantee holistic leakproofness, when needing to add culture solution or experimental cell, through aseptic tube injection can, effectively prevent that the cell from exposing for a long time, after the injection is accomplished, keep off and cover the pushing down, it is fixed to keep off the lid through the handle.

Description

Cell culture dish for research on microglial cell scorching

Technical Field

The utility model belongs to the technical field of medical treatment, especially, a cell culture dish for research of little glial cell burnt death.

Background

A petri dish is a laboratory vessel for the cultivation of microorganisms or cells, consisting of a flat disc-shaped bottom and a lid, generally made of glass or plastic.

The culture dish is one of cell experiment's important container, when carrying out cell experiment, need add new cell repeatedly and carry out permanent experiment, observes the sample, reachs important result, but the culture dish of using often in the laboratory only leans on fixed lid to seal, when cell or other culture solutions are added to needs at every turn, all can expose the experimental cell, easily infects bacterium etc. influence the experimental result accuracy.

SUMMERY OF THE UTILITY MODEL

Utility model purpose: provides a cell culture dish for studying the apoptosis of microglia, which solves the problems in the prior art.

The technical scheme is as follows: a cell culture dish for research on the burnt death of microglia comprises a culture dish body, wherein a sealing cover is arranged above the culture dish body, and a blocking cover is arranged on the sealing cover;

the sealing cover is provided with a plurality of empty grooves, injection tubes are arranged in the empty grooves, and each injection tube consists of a sterile tube and a tube cover;

the tube cover is clamped with the empty groove, and the sterile tube is in interference fit with the tube cover;

the sterile tube comprises a first tube body and a second tube body, the first tube body is fixedly connected with the tube cover, and the second tube body is in sliding fit with the first tube body.

In a further embodiment, the tube cap surface is provided with an annular groove, the sterile tube being disposed within the annular groove.

In a further embodiment, the bottom surface of the blocking cover is provided with a sealing ring, and the sealing ring is matched with the size of the annular groove.

In a further embodiment, a threaded hole is formed in the middle of the sealing cover, a through hole is formed in the blocking cover, a threaded rod is mounted at the end of the handle, and the threaded rod penetrates through the blocking cover and is in threaded connection with the sealing cover.

In a further embodiment, a rubber sleeve is sleeved on the outer side of the sealing cover and is connected with the culture dish body.

In a further embodiment, a boss is arranged at the top of the culture dish body, a plurality of anti-slip pads are arranged at the bottom of the culture dish body, and clamping grooves are formed among the anti-slip pads.

In a further embodiment, a clamping line is arranged inside the rubber sleeve, and the rubber sleeve is connected with the boss through the clamping line.

In a further embodiment, a base is installed at the bottom of the culture dish body, a plurality of protrusions are arranged in the base, and the protrusions are clamped with the clamping grooves.

Has the advantages that: the utility model provides a cell culture dish for research of little glial cell burnt die, put into the culture dish body with the experiment cell, then be connected sealed lid and culture dish body, avoid inside cell to be contaminated, culture dish body outer wall installs the boss, sealed lid outside cover has the rubber sleeve, the rubber sleeve inner wall has a plurality of joint lines, joint line and boss joint, increase the leakproofness between sealed lid and the culture dish body through the rubber sleeve, culture dish body installs the slipmat in the bottom, guarantee in the experiment that the culture dish body is stable, after the cell loading is accomplished, the culture dish body is put into the base, draw-in groove and protruding joint, the culture dish body is placed more stably, in order to prevent to open sealed lid at every turn, lead to the culture dish body in the cell to receive the pollution, install a plurality of injection syringes on the sealed lid, the injection syringe comprises tube cap and aseptic tube, be provided with the ring channel between aseptic tube and the tube cap, when the state of stewing, the sealing washer and the joint ring channel of installing bottom of block cover, guarantee holistic leakproofness, when need add culture solution or cell, can through aseptic tube injection, effectively prevent the cell exposure, after the injection is accomplished, the handle is pushed down, through the block cover is fixed for a long time.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a schematic structural view of the sealing cover of the present invention.

Fig. 3 is a schematic structural view of the sterile tube of the present invention.

FIG. 4 is a schematic view of the structure of the culture dish body of the present invention.

The reference signs are: 1. a handle; 2. a blocking cover; 3. an injection tube; 4. a sealing cover; 5. a culture dish body; 6. a base; 7. a threaded hole; 8. a sterile tube; 9. a rubber sleeve; 10. a tube cover; 11. a first pipe body; 12. a second tube body; 13. a boss; 14. a non-slip mat; 15. a clamping groove.

Detailed Description

In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention. It will be apparent, however, to one skilled in the art, that the present invention may be practiced without one or more of these specific details. In other instances, well-known features have not been described in order to avoid obscuring the invention.

As shown in fig. 1 to 4, a cell culture dish for studying the apoptosis of microglia is composed of a handle 1; 2. a blocking cover; 3. an injection tube; 4. a sealing cover; 5. a culture dish body; 6. a base; 7. a threaded hole; 8. a sterile tube; 9. a rubber sleeve; 10. a tube cover; 11. a first pipe body; 12. a second tube body; 13. a boss; 14. a non-slip mat; 15. a clamping groove.

As shown in fig. 1 to 4, the base 6 is arranged at a designated position, the culture dish body 5 is clamped with the base 6, the anti-slip pads 14 are bonded at the bottom of the culture dish body 5, the clamping grooves 15 are arranged between the anti-slip pads 14, the boss 13 is arranged at the top of the culture dish body 5, the sealing cover 4 is arranged on the culture dish body 5, the rubber sleeve 9 is sleeved on the outer side of the sealing cover 4, the injection tube 3 is arranged on the sealing cover 4, the tube cover 10 is arranged on the sealing cover 4, the sterile tube 8 is arranged in the middle of the injection tube 3, the first tube body 11 is connected with the tube cover 10, the second tube body 12 is in sliding fit with the first tube body 11, and the handle 1 penetrates through the blocking cover 2 and the threaded hole 7 formed in the sealing cover 4.

Preferably, in order to prevent the problem that the culture dish body 5 slips off when placed on the test bed, a non-slip mat 14 is installed at the bottom of the culture dish body 5.

As a preferred scheme, be provided with draw-in groove 15 between the slipmat 14, fixed through draw-in groove 15 joint between culture dish body 5 and the base 6, culture dish body 5 places more stably, and base 6 highly is higher than culture dish body 5, and the part is opaque, can satisfy the requirement of light-resistant when culture dish body 5 cultivates.

Preferably, in order to enhance the sealing performance between the sealing cover 4 and the culture dish body 5, a rubber sleeve 9 is sleeved outside the sealing cover 4, and the rubber sleeve 9 is tightly clamped with a boss 13 on the culture dish body 5.

As a preferable scheme, when new cells or culture solution needs to be added, the sealing cover 4 does not need to be completely opened like the conventional method, and only injection through the injection tube 3 is needed, so that the internal cells are prevented from being infected by bacteria.

As a preferred scheme, be provided with the ring channel between tube cap 10 and aseptic tube 8, when reinjecting into the culture solution, partial residue can flow into in the ring channel, and can not flow into aseptic tube 8, need when clearing up clean the ring channel can, keep off simultaneously that the lid 2 bottom is installed the sealing washer, sealing washer and ring channel size adaptation, better seal.

As a preferred scheme, the direct sliding fit of first body 11 and second body 12, when meetting the culture dish body 5 of the size adaptation, second body 12 can contract inside first body 11, shortens the distance between the body, can carry out interim injection work, strives for the time for new experimental apparatus.

Preferably, when new cells or culture solution is not needed to be injected, the handle 1 is connected with the threaded hole 7 on the sealing cover 4, and the blocking cover 2 is pressed tightly in the connection process to keep the whole sealing performance.

The utility model relates to a theory of operation that is used for burnt die research's of microglia cell to cultivate ware as follows:

put the experiment cell into culture dish body 5, then be connected sealed lid 4 and culture dish body 5, avoid inside cell to be contaminated, culture dish body 5 outer wall installs boss 13, sealed lid 4 outside cover has rubber sleeve 9, there are a plurality of joint lines rubber sleeve 9 inner wall, joint line and boss 13 joint, increase the leakproofness between sealed lid 4 and culture dish body 5 through rubber sleeve 9, culture dish body 5 installs slipmat 14 in the bottom, guarantee culture dish body 5 stable in the experiment, after the cell loading is accomplished, culture dish body 5 is put into base 6, draw-in groove 15 and protruding joint, culture dish body 5 places more stably, in order to prevent to open sealed lid 4 at every turn, lead to the cell to receive the pollution in the culture dish body 5, install a plurality of injection pipes 3 on sealed lid 4, injection pipe 3 comprises tube cover 10 and aseptic tube 8, be provided with the ring channel between aseptic tube 8 and tube cover 10, when the state of stewing, the sealing washer and the joint of installing bottom shield 2, guarantee holistic leakproofness, when need add culture solution or experiment cell, through aseptic tube 8, can prevent effectively injecting into the cell injection into, can prevent to press down 2 after the shield, after a long time, it is exposed, 2.

The preferred embodiments of the present invention have been described in detail with reference to the accompanying drawings, however, the present invention is not limited to the details of the above embodiments, and the technical solutions of the present invention can be subjected to various equivalent transformations within the scope of the technical idea of the present invention, and these equivalent transformations all belong to the protection scope of the present invention.

Claims

1. A cell culture dish for research on apoptosis of microglia comprises a culture dish body (5), and is characterized in that a sealing cover (4) is arranged above the culture dish body (5), and a blocking cover (2) is arranged on the sealing cover (4);

a plurality of empty grooves are formed in the sealing cover (4), injection pipes (3) are arranged in the empty grooves, and each injection pipe (3) consists of a sterile pipe (8) and a pipe cover (10);

the tube cover (10) is clamped with the empty groove, and the sterile tube (8) is in interference fit with the tube cover (10);

aseptic pipe (8) include first body (11) and second body (12), first body (11) with tube cap (10) fixed connection, second body (12) with first body (11) sliding fit.

2. A cell culture dish for use in the study of microglial apoptosis as claimed in claim 1, wherein: the surface of the pipe cover (10) is provided with an annular groove, and the sterile pipe (8) is arranged in the annular groove.

3. A cell culture dish for use in the study of apoptosis of microglia as claimed in claim 1, wherein: and a sealing ring is arranged on the bottom surface of the blocking cover (2), and the sealing ring is matched with the annular groove in size.

4. A cell culture dish for use in the study of microglial apoptosis as claimed in claim 1, wherein: the middle of the sealing cover (4) is provided with a threaded hole (7), the blocking cover (2) is provided with a through hole, the end of the handle (1) is provided with a threaded rod, and the threaded rod penetrates through the blocking cover (2) and the sealing cover (4) in threaded connection.

5. A cell culture dish for use in research of apoptosis of microglia as claimed in claim 4, wherein: the outer side of the sealing cover (4) is sleeved with a rubber sleeve (9), and the rubber sleeve (9) is connected with the culture dish body (5).

6. A cell culture dish for use in the study of microglial apoptosis as claimed in claim 1, wherein: the top of the culture dish body (5) is provided with a boss (13), a plurality of anti-slip pads (14) are installed at the bottom of the culture dish body (5), and a plurality of clamping grooves (15) are formed between the anti-slip pads (14).

7. A cell culture dish for use in research of apoptosis of microglia as claimed in claim 5, wherein: the rubber sleeve (9) is internally provided with clamping lines, and the rubber sleeve (9) is connected with the boss (13) through the clamping lines.

8. A cell culture dish for use in the study of apoptosis of microglia as claimed in claim 1, wherein: base (6) are installed to culture dish body (5) bottom, be provided with a plurality of archs in base (6), protruding and draw-in groove (15) joint.