CN213680726U

CN213680726U - Cell and tissue culture bioreactor

Info

Publication number: CN213680726U
Application number: CN202021103914.8U
Authority: CN
Inventors: 段美文
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-06-15
Filing date: 2020-06-15
Publication date: 2021-07-13
Anticipated expiration: 2030-06-15

Abstract

The utility model discloses a cell and tissue culture bioreactor relates to cell culture technical field, comprising a main body, the top of main part is provided with the apron, and the bottom of apron is provided with the spliced pole that extends to the main part inside, the inside of spliced pole is provided with the feed liquor pipe, and the inside of feed liquor pipe is provided with the spring, the one end of spring is provided with the card pearl, and the position department of card pearl and main part internal contact is provided with the recess, the inside of main part is provided with cultivates the groove, and just cultivates one side of groove and be provided with the feed liquor pipe that extends to the main part outside, the opposite side of cultivateing the groove is provided with the drain pipe, the bottom of apron is provided with. The utility model discloses a set up test tube and airtight lid, when examining its culture tank inside liquid, open airtight lid and carry out the sample test, it causes the infection or even death to the cell to reduce outside bacterium entering culture tank, reaches the survival rate effect that promotes cell culture.

Description

Cell and tissue culture bioreactor

Technical Field

The utility model relates to a cell culture technology and field, in particular to a cell and tissue culture bioreactor.

Background

At present, the common cell culture and tissue culture modes are divided into two modes, one mode is static culture, a culture bottle or a culture dish is usually used, and culture solution needs to be replaced periodically. Static culture systems are not able to keep the system constant, in particular microsystem culture; the other is perfusion culture, namely cells are inoculated in a reactor, and the system continuously provides culture solution for the cells or tissues to grow.

The apron connected mode on current cell and cultivation bioreactor and top is too loaded down with trivial details, is not convenient for clean its internal environment to and outside bacterium gets into the culture tank when detecting internal environment, and causes the cell to infect to dying, and a main part of present demand is simple and easy and detect convenient cell and tissue culture bioreactor to its culture tank interior liquid.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a: in order to solve the problems of simple connection mode of the main body and the cover plate and convenient cell and tissue culture bioreactor for detecting liquid in the culture tank, a cell and tissue culture bioreactor is provided.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a cell and tissue culture bioreactor, includes the main part, the top of main part is provided with the apron, and the bottom of apron is provided with the spliced pole that extends to the main part is inside, the inside of spliced pole is provided with the feed liquor pipe, and the inside of feed liquor pipe is provided with the spring, the one end of spring is provided with the card pearl, and blocks the position department of pearl and the inside contact of main part and be provided with the recess, the inside of main part is provided with cultivates the groove, and cultivates one side of groove and be provided with the feed liquor pipe that extends to the main part outside, the opposite side of cultivateing the groove is provided with the drain pipe, the bottom of apron is provided with the capping, and the top of apron is provided with the test tube that runs through the apron inside and extend to the capping bottom.

Preferably, the outer side of the spring engages the inner wall of the spring bore.

Preferably, the sealing cover is rotatably connected with the detection tube through a rotating shaft.

Preferably, the top end of the culture tank is connected and matched with the bottom end of the tank cover.

Preferably, a base is arranged at the bottom end of the main body.

Preferably, a sealing gasket is arranged at the contact position of the cover plate and the main body.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the utility model has the advantages that by arranging the detection tube and the closed cover, when the liquid in the culture tank is detected, the closed cover is opened for sampling detection, so that the external bacteria is prevented from entering the culture tank to cause infection or even death of the cells, and the effect of improving the survival rate of cell culture is achieved;

2. the utility model discloses a set up recess and card pearl, when apron and main part are dismantled, exert a vertical ascending power to the apron through the manual work, cause the spring shrink to its extrusion of calorie pearl through the recess edge, and make its apron and main part separation reach the effect of convenient dismantlement and installation.

Drawings

Fig. 1 is a schematic structural view of the present invention;

fig. 2 is a top view of the present invention;

fig. 3 is a schematic view of the connection between the main body and the cover plate of the present invention.

In the figure: 1. a main body; 2. connecting columns; 3. a cover plate; 4. a detection tube; 5. a sealing cover; 6. a handle; 7. a slot cover; 8. a culture tank; 9. a groove; 10. clamping the beads; 11. a spring; 12. a spring hole; 13. a liquid inlet pipe; 14. a liquid outlet pipe.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1 to 3, a cell and tissue culture bioreactor includes a main body 1, a cover plate 3 provided on the top of the main body 1, and the bottom end of the cover plate 3 is provided with a connecting column 2 extending to the inside of the main body 1, the inside of the connecting column 2 is provided with a liquid inlet pipe 13, a spring 11 is arranged inside the liquid inlet pipe 13, one end of the spring 11 is provided with a clamping bead 10, and a groove 9 is arranged at the position where the clamping bead 10 contacts with the inside of the main body 1, a culture tank 8 is arranged inside the main body 1, and one side of the culture tank 8 is provided with a liquid inlet pipe 13 extending to the outer side of the main body 1, the other side of the culture tank 8 is provided with a liquid outlet pipe 14, the bottom end of the cover plate 3 is provided with a tank cover 7, and the top of apron 3 is provided with and runs through the inside detection tube 4 that extends to the capping 7 bottom of apron 3, and the top of detection tube 4 is provided with airtight lid 5, and the top of airtight lid 5 is provided with handle 6.

Referring to fig. 1 and 3, the outer side of the spring 11 is engaged with the inner wall of the spring hole 12 to facilitate the connection between the cover plate and the main body, thereby protecting the healthy growth of the cells inside.

Please refer to fig. 1 and 2, the sealing cover 5 is rotatably connected to the detecting tube 4 via a rotating shaft, so as to facilitate manual detection of the liquid in the culture tank and ensure the healthy growth environment of the cells.

Please refer to FIGS. 1 and 2, the top of the culture tank 8 is engaged with the bottom of the cover 7 to prevent the liquid and cells from flowing out of the culture tank due to shaking during the movement of the reactor.

Please refer to fig. 1, the bottom of the main body 1 is provided with a base to facilitate manual transportation, and reduce the influence of damp or hot ground on the internal environment.

Please refer to fig. 1 and 2, a sealing gasket is disposed at a contact position of the cover plate 3 and the main body 1 to reduce the entry of external bacteria and protect the healthy living environment inside the culture tank.

The working principle is as follows: the using equipment is that firstly, the liquid at the outer side is injected into the culture tank 8 through the liquid inlet pipe 13, the cells cultured by the medicine are placed into the culture tank 8 for culturing, then the cover plate 3 is fixedly connected with the main body 1, a vertical downward force is manually exerted on the cover plate 3, the clamping beads 10 are extruded through the edge of the groove 9 to cause the springs 11 to contract, so that the clamping beads 10 enter the groove 9 to complete the fixed connection, when the liquid at the inner side is detected, the closed cover 5 is manually opened, the sample extraction equipment is placed into the detection pipe 4 for sampling detection, thereby reducing the infection and death of the cells caused by the external bacteria entering the culture tank 8, achieving the effect of improving the survival rate of cell culture, and when the cover plate 3 is detached and installed with the main body 1, a vertical upward force is manually exerted on the cover plate 3, the spring 11 is contracted by the extrusion of the edge of the groove 9 to the clamping bead, so that the cover plate 3 is separated from the main body 1 to achieve the effect of convenient disassembly and assembly.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. A cell and tissue culture bioreactor comprising a body (1), characterized in that: the device is characterized in that a cover plate (3) is arranged at the top end of a main body (1), a connecting column (2) extending to the inside of the main body (1) is arranged at the bottom end of the cover plate (3), a liquid inlet pipe (13) is arranged inside the connecting column (2), a spring (11) is arranged inside the liquid inlet pipe (13), a clamping bead (10) is arranged at one end of the spring (11), a groove (9) is arranged at the position where the clamping bead (10) is in contact with the inside of the main body (1), a culture tank (8) is arranged inside the main body (1), a liquid inlet pipe (13) extending to the outer side of the main body (1) is arranged on one side of the culture tank (8), a liquid outlet pipe (14) is arranged on the other side of the culture tank (8), a groove cover (7) is arranged at the bottom end of the cover plate (3), and a detection pipe (4) extending to the bottom end of the groove, the top end of the detection tube (4) is provided with a closed cover (5), and the top end of the closed cover (5) is provided with a handle (6).

2. A cell and tissue culture bioreactor according to claim 1, wherein: the outer side of the spring (11) is matched with the inner wall of the spring hole (12).

3. A cell and tissue culture bioreactor according to claim 1, wherein: the closed cover (5) is rotatably connected with the detection tube (4) through a rotating shaft.

4. A cell and tissue culture bioreactor according to claim 1, wherein: the top end of the culture tank (8) is connected and matched with the bottom end of the tank cover (7).

5. A cell and tissue culture bioreactor according to claim 1, wherein: the bottom end of the main body (1) is provided with a base.

6. A cell and tissue culture bioreactor according to claim 1, wherein: and a sealing gasket is arranged at the contact position of the cover plate (3) and the main body (1).