CN203947097U - Microorganism culturing reactive system - Google Patents

Microorganism culturing reactive system Download PDF

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Publication number
CN203947097U
CN203947097U CN201420226963.9U CN201420226963U CN203947097U CN 203947097 U CN203947097 U CN 203947097U CN 201420226963 U CN201420226963 U CN 201420226963U CN 203947097 U CN203947097 U CN 203947097U
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China
Prior art keywords
reactor
synthetic glass
glass groove
cultivation
microorganism culturing
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Expired - Lifetime
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CN201420226963.9U
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Chinese (zh)
Inventor
张宇
李介夫
肖湘
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Shanghai Jiaotong University
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Shanghai Jiaotong University
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Abstract

The utility model provides a kind of microorganism culturing reactive system, comprises medium container, peristaltic pump, cultivation reactor, gas cupboard, takes out air exchange system.The beneficial effects of the utility model: contain three pairs of parallelly cultivate reaction chambers, can realize complete parallel contrast in the experiment of electricity irritation class; Six cultivation reaction chambers can independently be cultivated, and different velocity of flow and Different electrodes surface shear power are provided; Keep for a long time gas-liquid closed and isolated chemistry and biological pollution; Realize nutrient solution sampling and electrode surface sampling in experimentation; The interface and the modular design that extensively adapt to, reequip, install additional specific electrode or miscellaneous part according to experiment demand easily; Mainly for the microorganism in Yu Haiyang source, be applicable to the multiple biological specimen including environmental samples, pure culture sample.

Description

Microorganism culturing reactive system
Technical field
The utility model relates to microorganism electrochemical technology and culture technique field, refers to especially a kind of microorganism culturing reactive system for Deep-Sea Microorganisms research.
Background technology
Microbiological fuel cell technology can become electricity collection by energy transformation contained organism in sewage, and utilize microorganism electrochemical reaction development of fuel cells, dispose of sewage etc. has great potential; Meanwhile, adopt electrochemical method microorganisms metabolic process and microorganism-environment to be subject to extensive concern as mechanism mutually; On the other hand, ocean, as understanding still shallow natural resources storehouse, has huge research and development potentiality, and the research of marine-derived microorganism is for explaining that microorganism-environment is made mechanism mutually, development of new natural resources has vital role.But, so far delivered or disclosed equipment scheme is studied based on traditional chemical mostly, research range is more single, be difficult to be applicable to the integration requirement to functions such as sampling in polymorphic type biological specimen suitability, cultured continuously, experiment in the research such as microorganism enrichment of marine source, become the major obstacles in marine-derived microorganism electrochemical research and application.
Utility model content
For defect of the prior art, the technical problems to be solved in the utility model is to provide a kind of microorganism culturing reactive system for Deep-Sea Microorganisms research, realize cultivate, in experiment in the multitask situation such as sampling, pollution-free in maintain system, can, according to different experiments, change the assemblies such as required electrode.
The utility model provides a kind of microorganism culturing reactive system, comprises medium container, peristaltic pump, cultivation reactor, gas cupboard, takes out air exchange system;
Described medium container is connected to cultivation reactor by peristaltic pump substratum is provided, and provides substratum to realize described medium container by peristaltic pump as cultivating reactor; Described gas cupboard is connected to cultivation reactor by taking out air exchange system, provides gas to realize described gas cupboard by taking out air exchange system as cultivating reactor.
Preferably, also comprise sampling system, wherein, described sampling system connects described cultivation reactor, and described sampling system is cultivated the culture of reactor for sampling.
Preferably, also comprise constant incubator, wherein, described cultivation reactor is arranged in described constant incubator, and described constant incubator is used to cultivation reactor to provide temperature adjustable environment.
Preferably, described cultivation reactor comprises exchange membrane, synthetic glass groove, silicone rubber O-ring, high pure graphite electrode, silicone rubber seal gasket, poly (methyl methacrylate) plate, screw, wherein, the both sides of described exchange membrane are symmetrical structure by screw respectively from inside to outside and fix successively organic glass guide channel, silicone rubber O-ring, high pure graphite electrode, silicone rubber seal gasket and poly (methyl methacrylate) plate.
Preferably, described cultivation reactor comprises multipair parallelly cultivate reaction chamber.
Preferably, described cultivation reaction chamber is the cultivation reaction chamber that can independently carry out cultured continuously.
Preferably, synthetic glass groove is provided with empties part, and the side of synthetic glass groove is provided with perforate, and what perforate had been communicated with synthetic glass groove empties part and the outside of synthetic glass groove;
Exchange membrane is distributed in the emptying between part of synthetic glass groove of its both sides;
The inner side of high pure graphite electrode is connected and sealed by the non-outside of emptying part of silicone rubber O-ring and synthetic glass groove;
The outside of high pure graphite electrode is connected and sealed by the inner side of silicone rubber seal gasket and poly (methyl methacrylate) plate.
Preferably, in the perforate that synthetic glass groove side arranges, fill in sillicon rubber blocking and realize the gas-liquid closed of synthetic glass groove;
Peristaltic pump is connected with common disposable aspiration needle by conduit, and syringe needle inserts in organic glass guide channel by sillicon rubber blocking, thereby connects peristaltic pump and cultivate reactor;
Take out air exchange system and be connected with common disposable aspiration needle by conduit, syringe needle inserts in organic glass guide channel by sillicon rubber blocking, takes out air exchange system and cultivates reactor thereby connect;
Sampling system connecting puncture needle, puncture needle inserts sillicon rubber blocking, thereby connects sampling system and cultivate reactor.
Compared with prior art, the utlity model has following beneficial effect:
1, contain three pairs of parallelly cultivate reaction chambers, in the experiment of electricity irritation class, can realize complete parallel contrast;
2, six cultivation reaction chambers can independently be cultivated, and different velocity of flow and Different electrodes surface shear power are provided;
3, keep for a long time gas-liquid closed and isolated chemistry and biological pollution;
4, realize sampling and electrode surface sampling in experimentation;
5, the interface and the modular design that extensively adapt to, reequip, install additional specific electrode or miscellaneous part according to experiment demand easily;
6, mainly for the microorganism in Yu Haiyang source, be applicable to the multiple biological specimen including environmental samples, pure culture sample.
Brief description of the drawings
By reading the detailed description of non-limiting example being done with reference to the following drawings, it is more obvious that other features, objects and advantages of the present utility model will become:
Fig. 1 is application principle block diagram of the present utility model.
Fig. 2 is the structural representation of cultivating reactor in Fig. 1.
Fig. 3 is the structural representation of poly (methyl methacrylate) plate in the utility model the first embodiment.
Fig. 4 is the structural parameter figure of poly (methyl methacrylate) plate in the utility model the first embodiment.
Fig. 5 is the structural representation of poly (methyl methacrylate) plate, silicone rubber seal gasket, high pure graphite electrode in the utility model the first embodiment, and wherein, dash area represents overlapping silicone rubber seal gasket and high pure graphite electrode.
Fig. 6 is the structural representation of synthetic glass groove in the utility model the first embodiment.
Fig. 7 is the structural parameter figure of synthetic glass groove in the utility model the first embodiment.
Fig. 8 is exchange membrane and overlapping synthetic glass groove and the structural representation of silicone rubber O-ring in the utility model the first embodiment, and wherein, dash area represents exchange membrane.
Fig. 9 illustrates the front view of cultivating reactor in the utility model second embodiment of structural parameter.
Figure 10 illustrates in the utility model second embodiment of structural parameter to cultivate reactor along dashdotted sectional view in Fig. 9.
Figure 11 illustrates the left view of cultivating reactor in the utility model second embodiment of structural parameter.
Figure 12 illustrates the top view of cultivating reactor in the utility model second embodiment of structural parameter.
In figure:
A is screw;
B is for emptying part;
C is lateral opening hole;
D is the outlet of high pure graphite electrode copper conductor;
E is wide adaptability interface;
F is the silicon rubber pad that 2mm is thick;
G is the poly (methyl methacrylate) plate that 18mm is thick;
H is the poly (methyl methacrylate) plate that 18mm is thick;
I is the silicon rubber pad that 2mm is thick.
Embodiment
Below in conjunction with specific embodiment, the utility model is elaborated.Following examples will contribute to those skilled in the art further to understand the utility model, but not limit in any form the utility model.It should be pointed out that to those skilled in the art, without departing from the concept of the premise utility, can also make some distortion and improvement.These all belong to protection domain of the present utility model.
The utility model provides a kind of microorganism culturing reactive system for Deep-Sea Microorganisms research for the deficiencies in the prior art, as shown in Figure 1, comprise medium container, peristaltic pump, cultivation reactor, gas cupboard, take out air exchange system, in an application, also dispose computer and electrochemical workstation.Described medium container is for providing substratum by peristaltic pump for cultivating reactor; Described gas cupboard is for providing gas by taking out air exchange system for cultivating reactor; Described computer is for input control order; Described electrochemical workstation is for detecting in real time the data parameters of cultivating reactor according to the steering order of computer input; Described electrochemical workstation is also for inputting electricity irritation according to the steering order of computer input for cultivating reactor; Described computer is also for the detected data parameters of store electricity chem workstation.
Described cultivation reactor is also connected with the sampling system of the culture of cultivating reactor for sampling.
Described cultivation reactor is also provided with the constant incubator that is used to cultivation reactor that the adjustable environment of temperature is provided outward.
As shown in Figure 2, described cultivation reactor comprises an exchange membrane 7, two synthetic glass grooves 6, two silicone rubber O-rings 5, two high pure graphite electrodes 4, two silicone rubber seal gaskets 3, two poly (methyl methacrylate) plates 2 and plus screw 1, described plus screw is the fixing reactor surrounding of cultivating respectively, and the both sides of described exchange membrane are symmetrical structure by screw respectively from inside to outside and fix successively organic glass guide channel, silicone rubber O-ring, high pure graphite electrode, silicone rubber seal gasket and poly (methyl methacrylate) plate.Poly (methyl methacrylate) plate is clamped synthetic glass groove, has formed together cultivation reaction chamber.
Particularly, synthetic glass groove is provided with empties part, and the side of synthetic glass groove is provided with perforate, and what perforate had been communicated with synthetic glass groove empties part and the outside of synthetic glass groove; Exchange membrane is distributed in the emptying between part of synthetic glass groove of its both sides; The inner side of high pure graphite electrode is connected and sealed by the non-outside of emptying part of silicone rubber O-ring and synthetic glass groove; The outside of high pure graphite electrode is connected and sealed by the inner side of silicone rubber seal gasket and poly (methyl methacrylate) plate.
More specifically, synthetic glass groove side is provided with perforate, and this perforate can be filled in sillicon rubber blocking and realize the gas-liquid closed of synthetic glass groove.The cultivation reactor parts being connected with peristaltic pump is the perforate of silicon rubber plug; Peristaltic pump is connected with common disposable aspiration needle by conduit, and syringe needle inserts in organic glass guide channel by sillicon rubber blocking, thereby connects peristaltic pump and cultivate reactor.Be the perforate of silicon rubber plug with taking out the cultivation reactor parts that air exchange system is connected; Take out air exchange system and be connected with common disposable aspiration needle by conduit, syringe needle inserts in organic glass guide channel by sillicon rubber blocking, takes out air exchange system and cultivates reactor thereby connect.The cultivation reactor parts being connected with sampling system is the perforate of silicon rubber plug; Sampling system connects lengthening stiffened puncture needle, and puncture needle inserts sillicon rubber blocking, thereby connects sampling system and cultivate reactor.Especially, by adjusting puncture needle angle and intubating length, can realize electrode surface or the sampling of culture specific region.
The cultivation reactor parts being connected with electrochemical workstation is high pure graphite electrode; High pure graphite electrode, containing a copper conductor, is connected electrode wires one end with copper conductor.The electrode wires the other end inserts in electrochemical workstation signal input/output port, thereby connects high pure graphite electrode and electrochemical workstation.
In a preferred embodiment, the cultivation reactor of the microorganism culturing reactive system that the utility model provides contains three pairs of parallelly cultivate reaction chambers, in the experiment of electricity irritation class, can realize complete parallel contrast; Six cultivation reaction chambers can independently carry out cultured continuously, different velocity of flow and Different electrodes surface shear power are provided, specifically, utilize the solution injection speed of six passage peristaltic pumps or six each cultivation reaction chambers of independent peristaltic pump control, select the syringe needle of different bores, regulator solution injection speed (six independent peristaltic pumps) to carry out control surface shearing force; Keep for a long time gas-liquid closed and isolated chemistry and biological pollution; Realize sampling and electrode surface sampling in experimentation; The interface and the modular design that extensively adapt to, reequip, install additional specific electrode or miscellaneous part according to experiment demand easily; For the microorganism of marine source, the multiple biological sample analysis including environmental samples, pure culture sample.
Carry out Shewanella piezotolerans WP3 bacterial strain to carry out cultured continuously, sampling and the detailed process of detection in real time for the microorganism electrochemical system that adopts the microorganism culturing reactive system that provides of the utility model and computer, electrochemical workstation to form below, step is as follows:
Step 1, by peristaltic pump by substratum (substratum composition: 34 grams of sodium-chlor, 5 grams of peptones, 1 gram of yeast cream adds distilled water to 1 liter) inject the cultivation reaction chamber of cultivating in reactor, this bacterial strain is moved in the cultivation reaction chamber of cultivating in reactor, carry out cultured continuously; In culture of continuous cultivation, constant incubator temperature is made as 37 DEG C; Open gas cupboard, provide cultivation required gas by taking out air exchange system for cultivating reactor, meanwhile, electrochemical workstation is inputted electricity irritation according to the steering order of computer input to cultivating reactor.
Incubation time: for a training mode, cultivate 72 hours; For cultured continuously pattern, incubation time needs decision according to experiment, and substratum flows with turnover rate on the 10%.
Electricity irritation voltage value: 1mV-50mV constant voltage
Electricity irritation object: for microorganisms cultures provides environment potential difference, thereby electron exchange and the metabolism of change microorganism and electrode (environment) are done mutually.
The ground wire electrode of electrochemical workstation is connected with the cultivation reaction chamber (blank chamber) that does not contain culture, and detecting electrode is connected with the cultivation reaction chamber (bacterium liquid chamber) containing bacterium liquid.
Step 2, electrochemical workstation detect the data parameters of cultivating reactor in real time according to the instruction of computer input.
Detect parameters: under impressed voltage pattern, electrochemical workstation can detect real-time current value (I-t figure), stream of electrons momentum aggregate-value (Q-t figure); Under non-impressed voltage pattern, electrochemical workstation can detect real-time electrical potential difference (V-t figure), real-time current value (I-t figure) and stream of electrons momentum aggregate-value (Q-t figure).
Step 3, in any period of cultured continuously, sample and cultivate the culture of reaction chamber by sampling system, it is detected.
For example: from reaction starts, near 200 microlitres of every two hours sampling electrode surface by sampling needle.The biochemical parameters that can be used for collecting sample detects (such as, NADPH/NADP measures), transcribe group analysis (such as, RNASeq analyzes), Proteomic analysis (such as, 2D-Gel analyzes).
It will be appreciated that; the technical scheme that the utility model is protected is only this hardware configuration of microorganism culturing reactive system; do not comprise computer and electrochemical workstation; above-mentioned microorganism electrochemical system etc. is in order to embody the application of the utility model in microorganism electrochemical system for example, instead of the utility model technical problem that can solve technique effect that maybe can obtain.
Above specific embodiment of the utility model is described.It will be appreciated that, the utility model is not limited to above-mentioned specific implementations, and those skilled in the art can make various distortion or amendment within the scope of the claims, and this does not affect flesh and blood of the present utility model.

Claims (7)

1. a microorganism culturing reactive system, is characterized in that, comprises medium container, peristaltic pump, cultivation reactor, gas cupboard, takes out air exchange system;
Described medium container is connected to cultivation reactor by peristaltic pump; Described gas cupboard is connected to cultivation reactor by taking out air exchange system.
2. microorganism culturing reactive system according to claim 1, is characterized in that, also comprises sampling system, and wherein, described sampling system connects described cultivation reactor.
3. microorganism culturing reactive system according to claim 1, is characterized in that, also comprises constant incubator, and wherein, described cultivation reactor is arranged in described constant incubator.
4. microorganism culturing reactive system according to claim 1 and 2, it is characterized in that, described cultivation reactor comprises exchange membrane, synthetic glass groove, silicone rubber O-ring, high pure graphite electrode, silicone rubber seal gasket, poly (methyl methacrylate) plate, screw, wherein, the both sides of described exchange membrane are symmetrical structure by screw respectively from inside to outside and fix successively organic glass guide channel, silicone rubber O-ring, high pure graphite electrode, silicone rubber seal gasket and poly (methyl methacrylate) plate.
5. microorganism culturing reactive system according to claim 4, is characterized in that, the poly (methyl methacrylate) plate that is positioned at exchange membrane both sides is clamped synthetic glass groove, has formed together cultivation reaction chamber, and described cultivation reactor comprises multipair parallelly cultivate reaction chamber.
6. microorganism culturing reactive system according to claim 4, is characterized in that,
Synthetic glass groove is provided with empties part, and the side of synthetic glass groove is provided with perforate, and what perforate had been communicated with synthetic glass groove empties part and the outside of synthetic glass groove;
Exchange membrane is distributed in the emptying between part of synthetic glass groove of its both sides;
The inner side of high pure graphite electrode is connected and sealed by the non-outside of emptying part of silicone rubber O-ring and synthetic glass groove;
The outside of high pure graphite electrode is connected and sealed by the inner side of silicone rubber seal gasket and poly (methyl methacrylate) plate.
7. microorganism culturing reactive system according to claim 6, is characterized in that,
In the perforate that synthetic glass groove side arranges, fill in sillicon rubber blocking and realize the gas-liquid closed of synthetic glass groove;
Peristaltic pump is connected with common disposable aspiration needle by conduit, and syringe needle inserts in organic glass guide channel by sillicon rubber blocking, thereby connects peristaltic pump and cultivate reactor;
Take out air exchange system and be connected with common disposable aspiration needle by conduit, syringe needle inserts in organic glass guide channel by sillicon rubber blocking, takes out air exchange system and cultivates reactor thereby connect;
Sampling system connecting puncture needle, puncture needle inserts sillicon rubber blocking, thereby connects sampling system and cultivate reactor.
CN201420226963.9U 2013-10-09 2014-05-05 Microorganism culturing reactive system Expired - Lifetime CN203947097U (en)

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CN201320620835.8 2013-10-09
CN201420226963.9U CN203947097U (en) 2013-10-09 2014-05-05 Microorganism culturing reactive system

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106867895A (en) * 2017-03-09 2017-06-20 宗春辉 Experiment on Microbiology anti-pollution type germ culturing device
CN107699483A (en) * 2017-10-30 2018-02-16 北华大学 A kind of medical test microbial cultivation equipment

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106867895A (en) * 2017-03-09 2017-06-20 宗春辉 Experiment on Microbiology anti-pollution type germ culturing device
CN107699483A (en) * 2017-10-30 2018-02-16 北华大学 A kind of medical test microbial cultivation equipment

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Granted publication date: 20141119