CN202116557U - Small-sized fermentation equipment - Google Patents
Small-sized fermentation equipment Download PDFInfo
- Publication number
- CN202116557U CN202116557U CN 201120096157 CN201120096157U CN202116557U CN 202116557 U CN202116557 U CN 202116557U CN 201120096157 CN201120096157 CN 201120096157 CN 201120096157 U CN201120096157 U CN 201120096157U CN 202116557 U CN202116557 U CN 202116557U
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- Prior art keywords
- small
- fermentation
- water bath
- sized
- sized fermentation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
Abstract
The utility model relates to small-sized fermentation equipment, which comprises a plurality of control units and fermentation units. Each control unit is provided with at least one pH (potential of hydrogen) controller, a plurality of acid and alkali solution holding measuring cylinders, a plurality of peristaltic pumps and a pinch valve, the pH controllers are connected with the peristaltic pump and the pinch valves. Each fermentation unit consists of a water bath, each water bath is provided with at least one multi-point magnetic stirrer and a circulating water heating controller, at least one group of small-sized fermentation tanks are further disposed in each water bath, and a feed inlet pipe of each small-sized fermentation tank runs through the corresponding peristaltic pump and the corresponding pinch valve to be respectively immersed in the acid and alkali solution holding measuring cylinder of the corresponding control unit. The small-sized fermentation equipment has the advantages of low equipment cost, small fermentation volume, convenience in operation and capability of simultaneously realizing multiple fermentation tests, can be used for fermenting microorganisms such as bacteria, fungi and the like, is applicable to medicine factories, biological factories, food factories and the like or laboratories of scientific research institutions, and is used for replacing current frequently-used shake flasks and conventional fermentation equipment.
Description
Technical field
The utility model relates to a kind of small-sized fermentation equipment that is specifically designed to products such as preparation bioenergy, biological medicine, biomaterial monomer, biological health-care food.
Background technology
In the microbial fermentation test, use the fermentor tank that shakes bottle or 3L-10L to verify the leavening property of bacterial strain usually, comprise product output, productive rate, product production intensity, microbial growth performance etc.Use shake bottle carry out fermentation test have simple to operate, the advantage that can carry out in enormous quantities, but shake bottle in culturing process, exist can not regulate the pH value, moisture evaporates, stirs shortcomings such as even inadequately easily.Therefore through data that shake flask test obtained when transferring to fermentor tank and verify, often have very big deviation.In addition, in tests such as microbe to screen, mikrobe physiological detection, often not very accurate through the data that shake flask test obtained yet.Though the conventional 3L-10L fermentor tank Data Detection of using is more accurate in the laboratory, can directly be used for scale-up, the fermentor tank of 3L-10L is often relatively heavier, the operation more complicated, and also equipment cost and running cost are all than higher.Each in addition test can only be used limited several jars usually, and parallel connection is used in batches, if will ferment detection in enormous quantities, needs could accomplish for a long time.Therefore can not satisfy user demand well.
Summary of the invention
To the problems referred to above, the main purpose of the utility model is to provide a kind of small-sized fermentation equipment, and it has, and equipment cost is low, fermentation volume is little, easy to operate, the advantage that can carry out a plurality of fermentation tests simultaneously.
For achieving the above object; A kind of small-sized fermentation equipment that the utility model provided; It is characterized in that comprising: some control units and fermentation unit; Said control unit has at least one pH unit, a plurality of soda acid liquid amount tube, a plurality of peristaltic pump and pinch valve, and said pH unit connects said peristaltic pump and pinch valve; Said fermentation unit comprises a water bath; An at least one multidigit point magnetic stirring apparatus and a recirculated water heating controller are arranged at said water bath; At least one group of small-sized fermentation jar also is set in said water bath, and the feed-pipe of each said small-sized fermentation jar immerses respectively behind said peristaltic pump and pinch valve in the soda acid liquid amount tube of said control unit.
In the technical scheme of above-mentioned the utility model; Said pH unit has a support body; One end of said support body is mounted with one group of pH meter; Each said pH meter connects the signal controller of each said peristaltic pump and pinch valve respectively, and said soda acid liquid amount tube, peristaltic pump and pinch valve all are arranged on the said support body.
The recirculated water heating controller of said fermentation unit is installed on the sidewall of said water bath, and said multidigit point magnetic stirring apparatus is arranged in the bottom or said water bath of said water bath.
The recirculated water heating controller of said fermentation unit is installed on the sidewall of said water bath, and said multidigit point magnetic stirring apparatus is arranged in the bottom or said water bath of said water bath.
Said small-sized fermentation jar has a tank body and a rubber plug; The bottom of said tank body is provided with stirrer; PH electrode wears and is fixed in said rubber plug; Also be equipped with a feed-pipe, a thief hole and two ventilating pits on the said rubber plug, said feed-pipe is immersed in the soda acid liquid amount tube of said control unit.
The heated perimeter of said recirculated water heating controller is 0～100 ℃, and the rotating speed of said multidigit point magnetic stirring apparatus is 0～1000rpm/min.
The heated perimeter of said recirculated water heating controller is 0～100 ℃, and the rotating speed of said multidigit point magnetic stirring apparatus is 0～1000rpm/min.
Adopt technique scheme, the characteristics that the utlity model has are: (1) pH unit respectively with the small-sized fermentation jar in pH electrode link to each other with peristaltic pump or pinch valve, control the pH value of fermentor tank through the soda acid liquid volume added; (2) place the small-sized fermentation jar of water bath, through recirculated water heating controller steady temperature; (3) multidigit point magnetic stirring apparatus makes thalline, nutritive medium uniform distribution in jar through the stirrer stir culture liquid in the control small-sized fermentation jar.This equipment has complete fermentation equipment assembly; Equipment cost is low, fermentation volume is little, easy to operate, running cost is low, a plurality of fermentation test is carried out simultaneously advantage; Can be used for carrying out microbial fermentations such as bacterium, fungi; Be applicable in the laboratory of factory such as medicine, biology, food or scientific research institution, and be used for replacing bottle and the conventional fermentation equipment of shaking commonly used at present.
Description of drawings
Fig. 1 is the structural representation of the utility model one preferred embodiment;
Fig. 2 a, Fig. 2 b are the structural representations of the control unit of the utility model one preferred embodiment;
Fig. 3 is the structural representation of the fermentation unit of the utility model one preferred embodiment;
Fig. 4 is the structural representation of the utility model fermentor tank.
Embodiment
Lift following examples at present and combine accompanying drawing that the structure and the effect of the utility model are elaborated.
Like Fig. 1～shown in Figure 3, the small-sized fermentation equipment that the utility model provided comprises control unit 10 and fermentation unit 20, and control unit 10 is made up of at least one pH unit 11, a plurality of soda acid liquid amount tube 12, a plurality of peristaltic pump 13 and pinch valve 14.Fermentation unit 20 comprises at least one multidigit point magnetic stirring apparatus 21, a recirculated water heating controller 22 and a water bath 23, and at least one group of small-sized fermentation jar 24 is set in water bath 23.
Like Fig. 1, shown in Figure 2; PH unit 11 has a support body 15; One end of support body 15 is mounted with one group of pH meter 16, and support body 15 is provided with one group of six pH meter 16 in the present embodiment, and each pH meter 16 connects the signal controller (not shown) of corresponding peristaltic pump 13 and pinch valve 14 respectively; Soda acid liquid amount tube 12 is arranged on the top of support body 15, and peristaltic pump 13 also is installed on the support body 15 with pinch valve 14.
As shown in Figure 3, the recirculated water heating controller 22 of fermentation unit 20 is fixedly installed on the sidewall of water bath 23, and the temperature heated perimeter is 0～100 ℃.Multidigit point magnetic stirring apparatus 21 is arranged in the bottom or water bath 23 of water bath 23, and its rotating speed can reach 0～1000rpm/min, is used for stirring the water of water bath 23, makes even water temperature.Each small-sized fermentation jar 24 is positioned in the water bath 23, and the water in the water bath 23 will soak the liquid nutrient medium in the small-sized fermentor tank 24.In the present embodiment, be placed with two groups of totally 12 small-sized fermentation jars 24 in the water bath 23.
As shown in Figure 4; Each small-sized fermentation jar 24 has a tank body 241 and a rubber plug 242; Liquid nutrient medium 243 is housed in the tank body 241; Be provided with stirrer 244 in the bottom of tank body 241, liquid amount is confirmed according to the size of small-sized fermentation jar 24, can adorn the liquid nutrient medium 243 of 100ml to 3000ml usually.PH electrode 245 wears and is fixed on the rubber plug 242, and pH electrode 245 is suspended in the tank body 241, and the end immerses in the liquid nutrient medium 243, and the lead on the pH electrode 245 is connected to the pH unit 11 of control unit 10.Also be equipped with a feed-pipe 246, one stopple coupons 247 and two ventilating pits on the rubber plug 242, one of them is air inlet port 248 for two ventilating pits, and another then is a production well 249.But feed-pipe 246 is the plastics tubing of acid and alkali-resistance, immerses in the soda acid liquid amount tube 12 of control unit 10 through peristaltic pump 13 and pinch valve 14 backs.
The working process of the utility model is: stirrer 244 is put in fermentor tank 24 bottoms in (1), behind high-temperature sterilization, adds an amount of sterilization fermentation liquid nutrient medium 243, and inserts fermented bacterium; (2) postvaccinal fermentor tank 24 is put into water bath 23, by recirculated water heating controller 22 controlled temperature, the multidigit that is positioned at water bath 23 bottoms is selected the rotating speed mixing fermented liquid of magnetic stirring apparatus 21 through control stirrer 244; (3) insert the pH electrode 245 of sterilizing at the top of fermentor tank 24; Each pH electrode 245 links to each other with pH meter 16 respectively and detects the pH value of fermented liquid in the fermenting process; When the pH value in the fermented liquid is below or above set(ting)value; PH meter 16 adds alkali or acid through the signal controller control peristaltic pump 13 or the pinch valve 14 of peristaltic pump or pinch valve, makes the pH value of fermented liquid keep constant during the fermentation.When the utility model was used for anaerobically fermenting, two ventilating pits on the rubber plug 242 sealed; When being used for aerobic fermentation, but air inlet port 248 bubbling airs in two ventilating pits, and 249 of production wells are discharged gas.
The quantity of the control unit 10 of the utility model and the quantity of small-sized fermentation jar 24 are complementary, and one group of small-sized fermentation jar 24 is joined a control unit 10.In the utility model the foregoing description, be provided with two groups of small-sized fermentation jars 24, the quantity of every group of small-sized fermentation jar 24 is 6, and corresponding, control unit 10 is two, and the pH meter 16 in each control unit 10 is set to 6.Certainly, the group number of the utility model control unit 10 and small-sized fermentation jar 24 is not as limit, and is same, and the quantity of every group of small-sized fermentation jar 24 is not exceeded with 6 yet, all can mate by actual demand.
Adopt said structure, the utlity model has following advantage:
1, equipment cost of the utility model and running cost low, have characteristics simple to operate, that can ferment in batches.Compare with the 5L fermentor tank of routine, the cost of each fermentor tank of small-sized fermentation equipment that the utility model provided is less than 1/5th of conventional fermentor tank, and fermentation can be carried out in batches at every turn, has saved great amount of time.
2, data accurately, can carry out scale-up.The fermentation data that the small-sized fermentation equipment that the utility model provided is obtained; Little with the data differences that fermentor tank obtained that the laboratory is conventional; Overcome traditional shake flask test owing to can not carry out the testing data that obtains that shortcomings such as pH regulator, moisture evaporation are bigger cause and have, be difficult to use in the defective in the scale-up than large deviation.
The above is merely the preferred embodiment of the utility model, and all equivalent transformations of doing according to the utility model specification sheets, claims and accompanying drawing all should be included in the scope of patent protection of the utility model.
Claims (7)
1. small-sized fermentation equipment; It is characterized in that comprising: some control units and fermentation unit; Said control unit has at least one pH unit, a plurality of soda acid liquid amount tube, a plurality of peristaltic pump and pinch valve, and said pH unit connects said peristaltic pump and pinch valve; Said fermentation unit comprises a water bath; An at least one multidigit point magnetic stirring apparatus and a recirculated water heating controller are arranged at said water bath; At least one group of small-sized fermentation jar also is set in said water bath, and the feed-pipe of each said small-sized fermentation jar immerses respectively behind said peristaltic pump and pinch valve in the soda acid liquid amount tube of said control unit.
2. small-sized fermentation equipment as claimed in claim 1; It is characterized in that: said pH unit has a support body; One end of said support body is mounted with one group of pH meter; Each said pH meter connects the signal controller of each said peristaltic pump and pinch valve respectively, and said soda acid liquid amount tube, peristaltic pump and pinch valve all are arranged on the said support body.
3. small-sized fermentation equipment as claimed in claim 1 is characterized in that: the recirculated water heating controller of said fermentation unit is installed on the sidewall of said water bath, and said multidigit point magnetic stirring apparatus is arranged in the bottom or said water bath of said water bath.
4. small-sized fermentation equipment as claimed in claim 2 is characterized in that: the recirculated water heating controller of said fermentation unit is installed on the sidewall of said water bath, and said multidigit point magnetic stirring apparatus is arranged in the bottom or said water bath of said water bath.
5. like claim 1 or 2 or 3 or 4 described small-sized fermentation equipment; It is characterized in that: said small-sized fermentation jar has a tank body and a rubber plug; The bottom of said tank body is provided with stirrer; PH electrode wears and is fixed in said rubber plug, also is equipped with a feed-pipe, a thief hole and two ventilating pits on the said rubber plug, and said feed-pipe is immersed in the soda acid liquid amount tube of said control unit.
6. like claim 1 or 2 or 3 or 4 described small-sized fermentation equipment, it is characterized in that: the heated perimeter of said recirculated water heating controller is 0～100 ℃, and the rotating speed of said multidigit point magnetic stirring apparatus is 0～1000rpm/min.
7. small-sized fermentation equipment as claimed in claim 5 is characterized in that: the heated perimeter of said recirculated water heating controller is 0～100 ℃, and the rotating speed of said multidigit point magnetic stirring apparatus is 0～1000rpm/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120096157 CN202116557U (en) | 2011-04-02 | 2011-04-02 | Small-sized fermentation equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120096157 CN202116557U (en) | 2011-04-02 | 2011-04-02 | Small-sized fermentation equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202116557U true CN202116557U (en) | 2012-01-18 |
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ID=45457638
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120096157 Expired - Lifetime CN202116557U (en) | 2011-04-02 | 2011-04-02 | Small-sized fermentation equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202116557U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105255728A (en) * | 2015-09-08 | 2016-01-20 | 中国环境科学研究院 | Indoor simulation device used for research of organic phosphorus enzyme hydrolysis process in water environment |
| CN110669647A (en) * | 2019-11-20 | 2020-01-10 | 佛山科学技术学院 | Laboratory microbial fermentation tank |
| CN111777438A (en) * | 2020-07-15 | 2020-10-16 | 曲靖凯美冠有机肥生产有限公司 | Nicotine liquid culture medium, application and activation fermentation device |
| CN113862147A (en) * | 2021-11-16 | 2021-12-31 | 陕西麦可罗生物科技有限公司 | Electromagnetic coupling embedded double-tank device and application thereof |
-
2011
- 2011-04-02 CN CN 201120096157 patent/CN202116557U/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105255728A (en) * | 2015-09-08 | 2016-01-20 | 中国环境科学研究院 | Indoor simulation device used for research of organic phosphorus enzyme hydrolysis process in water environment |
| CN110669647A (en) * | 2019-11-20 | 2020-01-10 | 佛山科学技术学院 | Laboratory microbial fermentation tank |
| CN111777438A (en) * | 2020-07-15 | 2020-10-16 | 曲靖凯美冠有机肥生产有限公司 | Nicotine liquid culture medium, application and activation fermentation device |
| CN113862147A (en) * | 2021-11-16 | 2021-12-31 | 陕西麦可罗生物科技有限公司 | Electromagnetic coupling embedded double-tank device and application thereof |
| CN113862147B (en) * | 2021-11-16 | 2024-02-27 | 陕西麦可罗生物科技有限公司 | Electromagnetic coupling embedded double-tank device and application thereof |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20120118 |