CN107034124A - A kind of system and its production method of acrylamide continuous production zymotic fluid - Google Patents
A kind of system and its production method of acrylamide continuous production zymotic fluid Download PDFInfo
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- CN107034124A CN107034124A CN201710269446.8A CN201710269446A CN107034124A CN 107034124 A CN107034124 A CN 107034124A CN 201710269446 A CN201710269446 A CN 201710269446A CN 107034124 A CN107034124 A CN 107034124A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/02—Percolation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
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Abstract
The present invention relates to a kind of system and its production method of acrylamide continuous production zymotic fluid, the system of production zymotic fluid includes being sequentially connected in series nutrient tank, one grade fermemtation culture tank, second order fermentation culture tank and the zymotic fluid storage tank of setting；Nutrient input in nutrient tank is delivered into one grade fermemtation culture tank, when Spawn incubation to biomass reaches 1.5~2.0mg/L in one grade fermemtation culture tank, various culture mediums are added from nutrient tank, the zymotic fluid in one grade fermemtation culture tank is conveyed into second order fermentation culture tank simultaneously, so as to further cultivate, obtained zymotic fluid delivers to the preservation of zymotic fluid storage tank.The advantage of the invention is that：Using once inoculation propagation, and pass through the flow velocity for the fresh medium for controlling to flow into incubator, the nutrient solution containing cell and metabolite in incubator is set also to be flowed out with substantially invariable flow velocity simultaneously, so that a kind of continuous production that the microorganism in fermentation tank is cultivated under conditions of cell density substantially constant is kept.
Description
Technical field
The invention belongs to microbial technology field, more particularly to a kind of acrylamide serialization
Produce the system and its production method of zymotic fluid.
Background technology
Acrylamide is a kind of white crystal chemical substance, is the raw material for producing polyacrylamide, is widely used in environment guarantor
Sewage disposal, oil three in shield are adopted, coating, fine chemistry industry and paper industry.
Current domestic microbiological method production acrylamide is to use traditional Intermittent fermentation technique, i.e. the fermentation per batch
Liquid, which is required for from being inoculated with, cultivates, grow into maturation puts tank these steps and repeats, so both waste of manpower, material resources, also not
Fermentation period can be shortened, and it is unstable per Batch fermentation liquid quality.
Therefore, research and develop it is a kind of can shorten fermentation period and can improve every Batch fermentation liquid quality acrylamide it is continuous
The system and method for metaplasia production zymotic fluid are necessary.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of system of acrylamide continuous production zymotic fluid and its life
Production method, is bred using once inoculation, and by the flow velocity for the fresh medium for controlling to flow into incubator, while making in incubator
The nutrient solution containing cell and metabolite also flowed out with substantially invariable flow velocity so that microorganism in fermentation tank is protecting
Hold a kind of continuous production cultivated under conditions of cell density substantially constant.
In order to solve the above technical problems, the technical scheme is that：A kind of acrylamide continuous production zymotic fluid
System, its innovative point is：Including being sequentially connected in series the nutrient tank of setting, one grade fermemtation culture tank, second order fermentation culture tank
With zymotic fluid storage tank；
Feed liquid charging aperture A of the top of the nutrient tank provided with a connection nutrient tank a, lower sidewall of nutrient tank is set
There is the feed liquid discharging port A of a connection nutrient tank；
Filtrated air mouthful of the top of the one grade fermemtation culture tank provided with a connection one grade fermemtation culture tank, one grade fermemtation culture
Feed liquid charging aperture B of one side wall upper part of tank provided with a connection one grade fermemtation culture tank, the opposite side wall of one grade fermemtation culture tank
Feed liquid discharging opening B of the bottom provided with a connection one grade fermemtation culture tank, the bottom of one grade fermemtation culture tank is provided with a connection one-level
The feed liquid discharging opening C of fermented and cultured tank；
The feed liquid discharging port A is connected with feed liquid charging aperture B by pipeline A, and the pipeline A has also been arranged in series a nutrient solution
Pump；The feed liquid discharging opening C is also connected by pipeline B and is additionally provided with a switch valve, the filter on a filter, and pipeline B
A lower sidewall provided with one connection filter feed liquid discharging opening D；
Feed liquid charging aperture E of the lower sidewall of second order fermentation culture tank one provided with a connection second order fermentation culture tank, and feed liquid
Charging aperture E is less than material liquid outlet B, material of another lower sidewall provided with a connection second order fermentation culture tank of second order fermentation culture tank
Liquid discharging opening E, and feed liquid discharging opening B connected with feed liquid charging aperture E by pipeline C；
Liquor inlet F, the material liquid outlet E and material of the top center of the zymotic fluid storage tank provided with a connection zymotic fluid storage tank
Liquid import F is connected by pipeline D.
A kind of production method of system with above-mentioned acrylamide continuous production zymotic fluid, its innovative point is：Institute
Production method is stated to comprise the following steps：
（1）It is in nutrient tank that fermentation medium and acrylamide is deployed after whole system sky disappears, nutrient solution is formed, then
Carry out real disappear；
（2）By nutrient pump by nutrient solution deployed in nutrient tank, squeeze into one grade fermemtation culture tank, keep one-level hair
27~29 DEG C of the temperature of nutrient solution in ferment culture tank, sterile sky is passed through by the filtrated air mouthful at the top of one grade fermemtation culture tank
Gas, throughput is：80m3/ h, pressure inside the tank is：0.05~0.08MPa, is then once inoculated with；
（3）Once after 52~54h of inoculation, the switch valve on opening conduits B samples in the material liquid outlet D of filter, works as one-level
When Spawn incubation to biomass reaches 1.5~2.0mg/L in fermented and cultured tank, the switch valve closed on pipeline B；
（4）At this point it is possible to the fermentation medium disappeared in fact with 1~2t/h of flow velocity additions from the liquor inlet of nutrient tank,
The zymotic fluid in one grade fermemtation culture tank utilizes difference in height simultaneously, also defeated from trend second order fermentation culture tank at a same speed
Send, so as to further cultivate；
（5）Broth temperature in second order fermentation culture tank is when 27~29 DEG C, pH value are 5.5~6.5, you can by second order fermentation
Zymotic fluid in culture tank delivers to the preservation of zymotic fluid storage tank.
The advantage of the invention is that：
（1）The system of acrylamide continuous production zymotic fluid of the present invention, is to be directed to conventional batch zymotechnique, process is numerous
It is trivial, it is improved, man power and material can be saved by the system of the acrylamide continuous production zymotic fluid, while can also
Shorten fermentation period, and be also stabilized per the quality of Batch fermentation liquid；
（2）The method of acrylamide continuous production zymotic fluid of the present invention, with reference to acrylamide continuous production of the present invention fermentation
The system of liquid, is bred using once inoculation, and by the flow velocity for the fresh medium for controlling to flow into incubator, while making incubator
In the nutrient solution containing cell and metabolite also with substantially invariable flow velocity flow out so that the microorganism in fermentation tank exists
Keep a kind of continuous production cultivated under conditions of cell density substantially constant；In addition, being passed through one grade fermemtation culture tank
In filtrated air be the air sterilized, to complement lysis oxygen in fermentation tank, be easy to fermented and cultured.
Brief description of the drawings
The present invention is further detailed explanation with reference to the accompanying drawings and detailed description.
Fig. 1 is the structural representation of the system of acrylamide continuous production zymotic fluid of the present invention.
Embodiment
The following examples can make professional and technical personnel that the present invention is more fully understood, but therefore not send out this
It is bright to be limited among described scope of embodiments.
Embodiment
The system of the present embodiment acrylamide continuous production zymotic fluid, as shown in figure 1, including being sequentially connected in series setting
Nutrient tank 1, one grade fermemtation culture tank 2, second order fermentation culture tank 3 and zymotic fluid storage tank 4.
Under feed liquid charging aperture 11 of the top of nutrient tank 1 provided with a connection nutrient tank 1, the side wall of nutrient tank 1
Feed liquid discharging opening 12 of the portion provided with a connection nutrient tank 1.
Filtrated air mouthful 21 of the top of one grade fermemtation culture tank 2 provided with a connection one grade fermemtation culture tank 2, one grade fermemtation
Feed liquid charging aperture 22 of one side wall upper part of culture tank 2 provided with a connection one grade fermemtation culture tank 2, one grade fermemtation culture tank 2
Feed liquid discharging opening 23 of another lower sidewall provided with a connection one grade fermemtation culture tank 2, the bottom of one grade fermemtation culture tank 2 is provided with
The feed liquid discharging opening 24 of one connection one grade fermemtation culture tank 2.
Feed liquid discharging opening 12 is connected with feed liquid charging aperture 22 by pipeline A, and the pipeline A has also been arranged in series a nutrition
Liquid pump 5；Feed liquid discharging opening 24 also connects a filter 6 by pipeline B, and a switch valve 7 is additionally provided with pipeline B, filter 6
Feed liquid discharging opening 61 of one lower sidewall provided with a connection filter.
Feed liquid charging aperture 31 of the side wall upper part of second order fermentation culture tank 3 one provided with a connection second order fermentation culture tank 3, and material
Liquid import 31 is less than feed liquid discharging opening 23, and another lower sidewall of second order fermentation culture tank 3 is provided with a connection second order fermentation culture
The feed liquid discharging opening 32 of tank 3, and feed liquid discharging opening 23 connected with feed liquid charging aperture 31 by pipeline C.
Liquor inlet 41 of the top center of zymotic fluid storage tank 4 provided with a connection zymotic fluid storage tank 4, material liquid outlet 32 and material
Liquid import 41 is connected by pipeline D.
The production method of the present embodiment system of above-mentioned acrylamide continuous production zymotic fluid, including following step
Suddenly：
（1）It is in nutrient tank 1 that fermentation medium and acrylamide is deployed after whole system sky disappears, nutrient solution is formed, then
Carry out real disappear；
（2）By nutrient pump 5 by nutrient solution deployed in nutrient tank 1, squeeze into one grade fermemtation culture tank 2, keep one
27~29 DEG C of the temperature of nutrient solution in level fermented and cultured tank 2, is led to by the filtrated air mouthful 21 at the top of one grade fermemtation culture tank 2
Enter filtrated air, throughput is：80m3/ h, pressure inside the tank is：0.05~0.08MPa, is then once inoculated with；
（3）Once after 52~54h of inoculation, the switch valve 7 on opening conduits B is sampled in the material liquid outlet 61 of filter 6, when one
When Spawn incubation to biomass reaches 1.5~2.0mg/L in level fermented and cultured tank 2, the switch valve 7 closed on pipeline B；
（4）At this point it is possible to the fermented and cultured disappeared in fact with 1~2t/h of flow velocity additions from the liquor inlet 11 of nutrient tank
Base, while the zymotic fluid in one grade fermemtation culture tank 2 utilizes difference in height, also at a same speed from trend second order fermentation culture tank
Conveyed in 3, so as to further cultivate；
（5）Broth temperature in second order fermentation culture tank 3 is when 27~29 DEG C, pH value are 5.5~6.5, you can by two grades of hairs
Zymotic fluid in ferment culture tank 3 is delivered to zymotic fluid storage tank 4 and preserved.
In order to protrude the advantage of the present invention, by the method and traditional batch of acrylamide continuous production zymotic fluid of the present invention
Formula zymotechnique is contrasted, and its comparing result see the table below：
| Project | Embodiment | Traditional zymotic technique |
| Labor intensity | 1. fermentation tank only needs sky to disappear, and disappears in fact respectively once；2. once it is inoculated with | 1. often produce once, just must sky disappear, real disappear respectively once；2. often producing a tank needs to be inoculated with once |
| Fermentation period | Once after 52~54h of inoculation, continuously there is zymotic fluid output for a long time | Every time inoculation 54h after output zymotic fluid 6t, 6t is produced again, need to be inoculated with again again, then etc. 54h |
| Zymotic fluid quality | It is stable, it is exactly a batch | Quality per 6t is different |
As can be seen from the above table, compared with traditional zymotic technique, the present invention changes traditional batch zymotechnique for continuous fermentation process,
And then man power and material can be saved, while fermentation period can also be shortened, and also it is stabilized per the quality of Batch fermentation liquid.
The general principle and principal character and advantages of the present invention of the present invention has been shown and described above.The skill of the industry
Art personnel are it should be appreciated that the present invention is not limited to the above embodiments, and described in above-described embodiment and specification is explanation
The principle of the present invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these
Changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and
Its equivalent thereof.
Claims (2)
1. a kind of system of acrylamide continuous production zymotic fluid, it is characterised in that：Nutrition including being sequentially connected in series setting
Flow container, one grade fermemtation culture tank, second order fermentation culture tank and zymotic fluid storage tank；
Feed liquid charging aperture A of the top of the nutrient tank provided with a connection nutrient tank a, lower sidewall of nutrient tank is set
There is the feed liquid discharging port A of a connection nutrient tank；
Filtrated air mouthful of the top of the one grade fermemtation culture tank provided with a connection one grade fermemtation culture tank, one grade fermemtation culture
Feed liquid charging aperture B of one side wall upper part of tank provided with a connection one grade fermemtation culture tank, the opposite side wall of one grade fermemtation culture tank
Feed liquid discharging opening B of the bottom provided with a connection one grade fermemtation culture tank, the bottom of one grade fermemtation culture tank is provided with a connection one-level
The feed liquid discharging opening C of fermented and cultured tank；
The feed liquid discharging port A is connected with feed liquid charging aperture B by pipeline A, and the pipeline A has also been arranged in series a nutrient solution
Pump；The feed liquid discharging opening C is also connected by pipeline B and is additionally provided with a switch valve, the filter on a filter, and pipeline B
A lower sidewall provided with one connection filter feed liquid discharging opening D；
Feed liquid charging aperture E of the lower sidewall of second order fermentation culture tank one provided with a connection second order fermentation culture tank, and feed liquid
Charging aperture E is less than material liquid outlet B, material of another lower sidewall provided with a connection second order fermentation culture tank of second order fermentation culture tank
Liquid discharging opening E, and feed liquid discharging opening B connected with feed liquid charging aperture E by pipeline C；
Liquor inlet F, the material liquid outlet E and material of the top center of the zymotic fluid storage tank provided with a connection zymotic fluid storage tank
Liquid import F is connected by pipeline D.
2. a kind of production method of the system of the acrylamide continuous production zymotic fluid described in use claim 1, its feature exists
In：The production method comprises the following steps：
(1) it is in nutrient tank that fermentation medium and acrylamide is deployed after whole system sky disappears, nutrient solution is formed, then
Carry out real disappear；
(2) by nutrient pump by nutrient solution deployed in nutrient tank, squeeze into one grade fermemtation culture tank, keep one-level hair
27~29 DEG C of the temperature of nutrient solution in ferment culture tank, sterile sky is passed through by the filtrated air mouthful at the top of one grade fermemtation culture tank
Gas, throughput is：80m3/ h, pressure inside the tank is：0.05~0.08MPa, is then once inoculated with；
(3) once after 52~54h of inoculation, the switch valve on opening conduits B samples in the material liquid outlet D of filter, works as one-level
When Spawn incubation to biomass reaches 1.5~2.0mg/L in fermented and cultured tank, the switch valve closed on pipeline B；
(4) at this point it is possible to add the fermentation medium disappeared in fact from the liquor inlet of nutrient tank with 1~2t/h of flow velocity,
The zymotic fluid in one grade fermemtation culture tank utilizes difference in height simultaneously, also defeated from trend second order fermentation culture tank at a same speed
Send, so as to further cultivate；
(5) broth temperature in second order fermentation culture tank is when 27~29 DEG C, pH value are 5.5~6.5, you can by second order fermentation
Zymotic fluid in culture tank delivers to the preservation of zymotic fluid storage tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710269446.8A CN107034124A (en) | 2017-04-24 | 2017-04-24 | A kind of system and its production method of acrylamide continuous production zymotic fluid |
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| CN201710269446.8A CN107034124A (en) | 2017-04-24 | 2017-04-24 | A kind of system and its production method of acrylamide continuous production zymotic fluid |
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| CN107034124A true CN107034124A (en) | 2017-08-11 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107746864A (en) * | 2017-11-25 | 2018-03-02 | 万达集团股份有限公司 | A kind of microbial method acrylamide new process for fermenting |
Citations (3)
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| CN1524961A (en) * | 2003-02-27 | 2004-09-01 | 上海双建生化技术发展有限公司 | Microorganism continuous catalysis method for producing acrylamide |
| CN102286560A (en) * | 2011-06-20 | 2011-12-21 | 山东宝莫生物化工股份有限公司 | Method for continuously producing acrylamide solution by using multi-level membrane bioreactors |
| CN106536745A (en) * | 2014-07-10 | 2017-03-22 | 三菱丽阳株式会社 | Method for producing compound and compound production system used in said production method |
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2017
- 2017-04-24 CN CN201710269446.8A patent/CN107034124A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1524961A (en) * | 2003-02-27 | 2004-09-01 | 上海双建生化技术发展有限公司 | Microorganism continuous catalysis method for producing acrylamide |
| CN102286560A (en) * | 2011-06-20 | 2011-12-21 | 山东宝莫生物化工股份有限公司 | Method for continuously producing acrylamide solution by using multi-level membrane bioreactors |
| CN106536745A (en) * | 2014-07-10 | 2017-03-22 | 三菱丽阳株式会社 | Method for producing compound and compound production system used in said production method |
Non-Patent Citations (1)
| Title |
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| 孙旭东等: "六级连续化膜生物反应工艺在丙烯酰胺生产中的应用研究", 《现代化工》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107746864A (en) * | 2017-11-25 | 2018-03-02 | 万达集团股份有限公司 | A kind of microbial method acrylamide new process for fermenting |
| CN107746864B (en) * | 2017-11-25 | 2021-08-06 | 万达集团股份有限公司 | Novel acrylamide fermentation process by microbiological method |
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