CN210959728U - Liquid strain fermentation device for edible fungi - Google Patents

Liquid strain fermentation device for edible fungi Download PDF

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Publication number
CN210959728U
CN210959728U CN201921821894.5U CN201921821894U CN210959728U CN 210959728 U CN210959728 U CN 210959728U CN 201921821894 U CN201921821894 U CN 201921821894U CN 210959728 U CN210959728 U CN 210959728U
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China
Prior art keywords
inoculation
fermentation
tank
pipe
fermentation tank
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Expired - Fee Related
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CN201921821894.5U
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Chinese (zh)
Inventor
黄启林
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Yichang Yuhe Edible Co ltd
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Yichang Yuhe Edible Co ltd
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Priority to CN201921821894.5U priority Critical patent/CN210959728U/en
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Abstract

The utility model relates to a liquid bacterial fermentation device of domestic fungus, including the fermentation cylinder, fermentation tank top feed inlet and seed liquid culture tank intercommunication, the bottom is equipped with the aeration ring canal in the fermentation cylinder, aeration ring canal top is equipped with the draft tube, the draft tube passes through the connecting rod to be fixed inside the fermentation tank, the aeration ring canal passes through pipeline and the outer gas holder intercommunication of fermentation tank, be equipped with filter and heater between gas holder and the fermentation cylinder in proper order, seed liquid culture tank pan feeding mouth is equipped with the inoculation device, the aeration ring canal includes first gas guide ring canal and second gas guide ring canal, and first gas guide ring canal diameter is less than the draft tube diameter, and second gas guide ring canal diameter is greater than the draft tube diameter. The utility model discloses realize that the hierarchical gradual expansion of domestic fungus liquid bacterial cultivates, the stirring of material and the provision of oxygen.

Description

Liquid strain fermentation device for edible fungi
Technical Field
The utility model belongs to the technical field of big ball lid mushroom production, a liquid bacterial fermenting installation of domestic fungus is related to.
Background
The fermentation tank of edible fungus is a fermentation facility equipment for producing liquid spawn for culturing edible fungus mycelium, and provides the mycelium with nutrients, pH value, temperature and oxygen required for growth by means of biological fermentation principle, so as to create an optimal growth environment for the mycelium and make the mycelium grow fast and propagate rapidly. Liquid strains have higher requirements on equipment, environment, cultivation materials and the like, particularly, the strains cannot be polluted, and if the strains are polluted, all the strains are scrapped, so the requirements on the environment are very high, and in the preparation process of the liquid strains, inoculation is usually carried out manually, so that hidden dangers are brought to the polluted strains. CN201820487733.6 liquid bacterial fermentation cylinder, when the rotation axis rotated, can drive first, second, third and fourth stirring leaf and rotate, can avoid the bacterial adhesion at the inner wall of fermentation cylinder body to and on the outer wall of the first jar of body, make the fermentation cylinder easily wash. Can evenly stir the bacteria liquid, and the temperature in the fermentation tank is convenient to adjust, and the fermentation tank is easy to clean. However, mechanical stirring is adopted, and the hyphae are easily damaged due to the mechanical shearing force of the stirrer. Is not suitable for edible fungi. CN201821251380.6 quick fermentation cylinder of domestic fungus liquid bacterial, through the aeration pipe that sets up the inboard bottom position of the fermentation tank body, and seted up a plurality of gas outlets on the aeration pipe, and gas outlet department welding has the air outlet mouth, send into the aeration pipe with outside air pressurization through air compressor in, spout by the air outlet mouth again. Play and provide required oxygen of fermentation and nitrogen source, also strengthen the inside stirring effect of fermentation tank simultaneously, but its aeration pipe is located the bottom, and the logical oxygen volume is limited, and in the bacterial culture process of edible fungi again, the hypha viscosity increases, and the pure supply of relying on the bottom air current can not play fine stirring effect.
Disclosure of Invention
In order to solve the technical problem, the utility model provides a liquid strain fermentation device of edible fungi, which reduces the pollution of mixed fungi and provides suitable oxygen in the growth process of mycelium.
The scheme of the utility model is that:
the utility model provides a liquid bacterial fermentation device of domestic fungus, the device includes the fermentation cylinder, fermentation tank top feed inlet and seed liquid culture tank intercommunication, the bottom is equipped with the aeration ring canal in the fermentation cylinder, aeration ring canal top is equipped with the draft tube, the draft tube passes through the connecting rod to be fixed inside the fermentation cylinder, the aeration ring canal passes through pipeline and the outer gas holder intercommunication of fermentation cylinder, be equipped with filter and heater between gas holder and the fermentation cylinder in proper order, seed liquid culture tank pan feeding mouth is equipped with the inoculation device, the aeration ring canal includes first gas guide ring canal and second gas guide ring canal, and first gas guide ring canal diameter is less than the draft tube diameter, and second gas guide ring canal diameter is greater than the draft tube diameter.
Preferably, the inoculation device comprises an inoculation platform, the inoculation pipe penetrates through the inoculation platform to be communicated with the fermentation tank, and an annular groove is formed in the inoculation platform and located on the outer side of the inoculation pipe.
Further preferably, a detachable inoculation cover is arranged on the inoculation table and used for covering the inoculation tube.
Still further preferably, a first valve is arranged above the inoculation cover, a second valve is arranged between the inoculation platform and the fermentation tank, and a third valve is arranged above the inoculation pipe and the inoculation platform.
Preferably, the blast pipe that the fermentation cylinder top was equipped with extends to the liquid below of seal groove, and the liquid top in the seal groove is equipped with the breather pipe.
Preferably, a steam inlet pipe is arranged above the top of the fermentation tank, and an interlayer is arranged on the surface of the tank body of the fermentation tank.
Preferably, the gas storage tank is also communicated with the seed liquid culture tank through a pipeline.
Preferably, the bottom of the fermentation tank is communicated with the temporary storage tank through a pipeline.
The utility model discloses beneficial effect:
1. the utility model discloses a fermentation cylinder top feed inlet and seed liquid culture tank intercommunication realize the hierarchical progressively enlarge cultivation of domestic fungus liquid spawn. The upper part of the aeration ring pipe is provided with a guide cylinder, the guide cylinder is used for guiding a gas path, the gas storage tank sequentially passes through a filter and a heater before entering a fermentation tank, air is filtered and heated to a temperature in a range of 23-25 ℃ suitable for edible fungi, and then the edible fungi enter the fermentation tank for growth of strains, the hypha of the edible fungi continuously absorbs nutrient components and oxygen in nutrient solution, so that nutrient substances and oxygen in the nutrient solution are continuously reduced, and therefore insufficient nutrition occurs at the later stage and oxygen deficiency inside the hypha is caused, so that the formation of a fungus ball is influenced. The flow paths of the materials in the fermentation tank entering the guide cylinder are provided with two opposite circulation paths by adjusting the introduction and switching of the gas in the first gas guide ring pipe and the second gas guide ring pipe, so that the stirring of the materials and the provision of oxygen are realized.
2. The seed liquid culture tank is provided with an inoculation device at a feeding port, so that the introduction of mixed bacteria in the inoculation operation is avoided, the annular groove is used for containing alcohol, and the alcohol is ignited in the inoculation process to realize aseptic operation.
3. The inoculation cover is used for covering the inoculation pipe, and the inoculation pipe is prevented from being polluted before or after inoculation.
4. Through adjusting the closure of the first valve, the second valve and the third valve, pollution is guaranteed to be generated before and after inoculation, the first valve, the second valve and the third valve are opened before inoculation, steam sterilization is carried out on a fermentation tank and a seed liquid culture tank, after the sterilization is finished, alcohol on the annular groove is ignited, the inoculation cover is opened to carry out edible fungus liquid spawn inoculation, after the inoculation is finished, the first valve, the second valve and the third valve are closed, the inoculation cover is closed, aseptic operation of inoculation is achieved, and aseptic inoculation in the spawn culture process is guaranteed.
5. The blast pipe that the fermentation cylinder top was equipped with extends to the liquid below of seal groove, and the liquid top in the seal groove is equipped with the breather pipe. Gas generated in the fermentation process is discharged through the liquid seal, so that foreign bacteria introduced at the exhaust pipe are avoided.
6. The top of the fermentation tank is provided with a steam inlet pipe, the tank body is sterilized by steam before fermentation inoculation, and the surface of the tank body of the fermentation tank is provided with an interlayer for controlling the stability of fermentation temperature.
7. The fermentation cylinder bottom is passed through the pipeline and is communicated with the jar of keeping in, and after the fermentation, the zymotic fluid is not direct to inoculate the fungus bag, but puts into the jar of keeping in earlier and inoculates the fungus bag again, avoids inoculating the fungus bag in-process and introduces miscellaneous fungus to the liquid in the fermentation cylinder.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the structure of the aeration ring pipe of the present invention;
FIG. 3 is a schematic view of the structure of the inoculating device of the present invention;
wherein: the fermentation tank 1, the steam inlet pipe 101, the interlayer 102, the seed liquid culture tank 2, the aeration ring pipe 3, the first air guide ring pipe 301, the second air guide ring pipe 302, the guide cylinder 4, the air storage tank 5, the filter 6, the heater 7, the inoculation device 8, the inoculation platform 801, the inoculation pipe 802, the annular groove 803, the inoculation cover 804, the first valve 805, the second valve 806, the third valve 807, the sealing groove 9 and the temporary storage tank 10 are communicated.
Detailed Description
The invention is further described with reference to the following examples, but the scope of the invention as claimed is not limited to the scope of the examples.
Example 1
As shown in fig. 1-2, a liquid strain fermentation device for edible fungi, the device comprises a fermentation tank 1, a feed inlet at the top of the fermentation tank 1 is communicated with a seed solution culture tank 2, an aeration ring pipe 3 is arranged at the bottom in the fermentation tank 1, a guide cylinder 4 is arranged above the aeration ring pipe 3, the guide cylinder 4 is fixed in the fermentation tank 1 through a connecting rod, the aeration ring pipe 3 is communicated with a gas storage tank 5 outside the fermentation tank 1 through a pipeline, a filter 6 and a heater 7 are sequentially arranged between the gas storage tank 5 and the fermentation tank 1, a feed inlet of the seed solution culture tank 2 is provided with an inoculation device 8, the aeration ring pipe 3 comprises a first gas guide ring pipe 301 and a second gas guide ring pipe 302, the diameter of the first gas guide ring pipe 301 is smaller than that of the guide cylinder 4, and the diameter of the.
Preferably, the inoculation device 8 comprises an inoculation platform 801, a inoculation pipe 802 penetrates through the inoculation platform 801 to be communicated with the fermentation tank 1, and the inoculation platform 801 is provided with an annular groove 803 on the outer side of the inoculation pipe 802.
Further preferably, a detachable inoculation cover 804 is arranged on the inoculation table 801, and the inoculation cover 804 is used for covering the inoculation pipe 802.
Still more preferably, a first valve 805 is provided above the inoculation cover 804, a second valve 806 is provided between the inoculation station 801 and the fermentation tank 1, and a third valve 807 is provided above the inoculation station 801 for the inoculation pipe 802.
Preferably, the exhaust pipe that the fermentation cylinder 1 top was equipped with extends to the liquid below of seal groove 9, and the liquid top in seal groove 9 is equipped with the breather pipe.
Preferably, a steam inlet pipe 101 is arranged above the top of the fermentation tank 1, and an interlayer 102 is arranged on the surface of the tank body of the fermentation tank 1.
Preferably, the gas storage tank 5 is also communicated with the seed liquid culture tank 2 through a pipeline.
Preferably, the bottom of the fermentation tank 1 is communicated with the temporary storage tank 10 through a pipeline.
Adopt the utility model is used for the preparation of oyster mushroom liquid bacterial: before inoculation and before fermentation inoculation, a first valve 805, a second valve 806 and a third valve 807 are opened, a fermentation tank and a seed liquid culture tank are subjected to steam sterilization, after the sterilization is finished, a culture medium is added into the fermentation tank 1 and the seed liquid culture tank 2, alcohol on the annular groove 803 is ignited, an inoculation cover is opened for edible fungus liquid strain inoculation, and after the inoculation is finished, the first valve 805, the second valve 806 and the third valve 807 are closed, and the inoculation cover is closed. The strain is subjected to primary culture in seed liquid culture tank 2 (at 23-25 deg.C, tank pressure of 0.02-0.04MPa, and ventilation amount of 5-20m3H, the time is 80-100h), after the primary culture technology, a third valve (807) is opened, the seed liquid enters a fermentation tank 1 for fermentation and expanded culture (the temperature is 23-25 ℃, the tank pressure is 0.02-0.04MPa, and the air flow is controlled to be 10-25m3And h, the time is 80-100h), in the fermentation process, the oyster mushroom mycelia continuously absorb nutrient components and oxygen in the fermentation liquid, so that the nutrient substances and the oxygen in the fermentation liquid are continuously reduced, and two opposite circulation paths are formed in the flow path of the materials in the fermentation tank entering the guide cylinder by introducing and switching the adjusting gas into the first gas guide ring pipe 301 and the second gas guide ring pipe 302, so that the stirring of the materials and the supply of the oxygen are realized, the mechanical damage to mycelium histiocytes is avoided, and the strain activity is improved. Fermenting for 60-80 hr to obtain mycelia with good growth rateThe liquid spawn of the edible fungi is transferred to a temporary storage tank 10 for preparation of inoculation of the fungi bags. Wherein the culture medium in the oyster mushroom seed liquid culture tank 2 is a potato culture medium, and the culture medium formula of the fermentation tank 2 is as follows: 40% of potato, 20% of bran, 20% of glucose, 8% of peptone, 9% of beef extract and KH2PO41.5%,MgSO41.5 percent. Adjusting the pH value to 6-6.5.

Claims (8)

1. A liquid strain fermentation device for edible fungi is characterized in that: the device comprises a fermentation tank (1), a feed inlet at the top of the fermentation tank (1) is communicated with a seed liquid culture tank (2), an aeration ring pipe (3) is arranged at the bottom in the fermentation tank (1), a guide cylinder (4) is arranged above the aeration ring pipe (3), the guide cylinder (4) is fixed inside the fermentation tank (1) through a connecting rod, the aeration ring pipe (3) is communicated with an air storage tank (5) outside the fermentation tank (1) through a pipeline, a filter (6) and a heater (7) are sequentially arranged between the gas storage tank (5) and the fermentation tank (1), the seed liquid culture tank (2) pan feeding mouth is equipped with inoculation device (8), aeration ring pipe (3) are including first air guide ring pipe (301) and second air guide ring pipe (302), and first air guide ring pipe (301) diameter is less than draft tube (4) diameter, and second air guide ring pipe (302) diameter is greater than draft tube (4) diameter.
2. The apparatus for fermenting liquid spawn of edible fungus according to claim 1, wherein: the inoculation device (8) comprises an inoculation table (801), the inoculation pipe (802) penetrates through the inoculation table (801) to be communicated with the fermentation tank (1), and an annular groove (803) is formed in the position, located on the outer side of the inoculation pipe (802), of the inoculation table (801).
3. The apparatus for fermenting liquid spawn of edible fungus according to claim 2, wherein: the inoculation table (801) is provided with a detachable inoculation cover (804), and the inoculation cover (804) is used for covering the inoculation pipe (802).
4. The apparatus for fermenting liquid spawn of edible fungus according to claim 3, wherein: a first valve (805) is arranged above the inoculation cover (804), a second valve (806) is arranged between the inoculation table (801) and the fermentation tank (1), and a third valve (807) is arranged above the inoculation table (801) of the inoculation pipe (802).
5. The apparatus for fermenting liquid spawn of edible fungus according to claim 1, wherein: the exhaust pipe that fermentation cylinder (1) top was equipped with extends to the liquid level below of seal groove (9), and the liquid level top in seal groove (9) is equipped with the breather pipe.
6. The apparatus for fermenting liquid spawn of edible fungus according to claim 1, wherein: a steam inlet pipe (101) is arranged above the top of the fermentation tank (1), and an interlayer (102) is arranged on the surface of the tank body of the fermentation tank (1).
7. The apparatus for fermenting liquid spawn of edible fungus according to claim 1, wherein: the gas storage tank (5) is also communicated with the seed liquid culture tank (2) through a pipeline.
8. The apparatus for fermenting liquid spawn of edible fungus according to claim 1, wherein: the bottom of the fermentation tank (1) is communicated with the temporary storage tank (10) through a pipeline.
CN201921821894.5U 2019-10-28 2019-10-28 Liquid strain fermentation device for edible fungi Expired - Fee Related CN210959728U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201921821894.5U CN210959728U (en) 2019-10-28 2019-10-28 Liquid strain fermentation device for edible fungi

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201921821894.5U CN210959728U (en) 2019-10-28 2019-10-28 Liquid strain fermentation device for edible fungi

Publications (1)

Publication Number Publication Date
CN210959728U true CN210959728U (en) 2020-07-10

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201921821894.5U Expired - Fee Related CN210959728U (en) 2019-10-28 2019-10-28 Liquid strain fermentation device for edible fungi

Country Status (1)

Country Link
CN (1) CN210959728U (en)

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20200710

Termination date: 20211028

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