CN104017723B - Edible fungi liquid strain transport inoculation tank - Google Patents
Edible fungi liquid strain transport inoculation tank Download PDFInfo
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- CN104017723B CN104017723B CN201410218750.6A CN201410218750A CN104017723B CN 104017723 B CN104017723 B CN 104017723B CN 201410218750 A CN201410218750 A CN 201410218750A CN 104017723 B CN104017723 B CN 104017723B
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- tank body
- tank
- basin
- inoculator
- air filter
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
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Abstract
The invention discloses edible fungi liquid strain transport inoculation tank, be made up of basin, air filter inoculator and air compressor machine.Basin is made up of tank body, cover and tank pin.Basin is provided with fluid inlet, inlet mouth, exhaust endless tube, discharge outlet, water port and bacterial classification delivery port, cover is provided with handheld ring, tensimeter, vent valve and band lamp window, has seal washer between cover and tank body, fixed by screw; The various microorganism of air filter energy filtering, its inlet end connects with the aspirating end of air compressor machine, and exhaust side connects with the interface of the inlet mouth of basin; Inoculator is injection bowl inoculator, connects with the interface of the bacterial classification delivery port of basin.The very large of liquid-spawn inoculation range expansion can be made after adopting the present invention, greatly reduce the investment of liquid culture production equipment, change the technical process of Edible Fungi plantation family Edible Fungi on a small scale, greatly shorten the edible fungus culturing cycle, improve economic benefit greatly.
Description
Technical field
The present invention relates to technical field of edible fungi production, particularly for the device of edible fungi liquid strain packed and transported.
Background technology
At present, edible fungi liquid strain is edible fungus species production technology comparatively advanced both at home and abroad, there is energy-saving and emission-reduction, environmental protection, the advantage that efficient and convenient, success ratio is high, therefore, at home and abroad be applied to a certain extent, but its application generally all adopt now do existing use, scene does on-the-spot mode, popularity rate is also not bery high, its major cause is: (1) facility investment is comparatively large, has certain technical difficulty; (2) liquid spawn produced is difficult to transport of guaranteeing the quality at a distance.Prior art Problems existing causes the application of liquid spawn to be confined to possess the R&D units of good condition or the manufacturing enterprise of large edible bacterium batch production, and is difficult to be widely applied in most Edible Fungi person and middle-size and small-size Edible Fungi enterprise.
Edible fungi liquid strain can long distance transportation, use, have three key issues to need to solve, one be liquid spawn under normal temperature or controlled temperature conditions, extend the guaranteeing the quality staging life of liquid spawn; Two is the edible fungi liquid strain containers adopting safely, be suitable for vehicle long distance transportation; Three is input and output that liquid spawn container facilitates liquid spawn.CN102267590A discloses a kind of edible fungi liquid strain packing bag device and application method thereof, this invention is packed liquid spawn by adopting a kind of method for packaging plastic bags, solve liquid spawn from centralized production to dispersion use two links exist packaging, transport, storage problem, there is the advantages such as packaging operation is easy, cost of investment is lower, but, with the container of plastics bag as liquid spawn storage and transport, be easy to damage, and be easy to when inoculating in point of destination pollute.
Summary of the invention
The object of the invention is the deficiency existed for prior art, a kind of edible fungi liquid strain is provided to transport inoculation tank, this transport inoculation tank can under normal temperature or controlled temperature conditions, extend the quality guaranteed period of liquid spawn, be suitable for long-time, remote vehicle transport, facilitate the input and output of liquid spawn, not easily pollute during operation.
For achieving the above object, the present invention adopts following technical scheme.
Edible fungi liquid strain transport inoculation tank is made up of basin, air filter inoculator and air compressor machine.
Described basin by bottom be circular arc, top is provided with the tank body of flange connector, the round end socket shape cover being provided with flange connector, the tank pin that is fixed on basin bottom are formed.Be provided with fluid inlet on the top of basin side, fluid inlet has pipeline through to close to pot bottom in tank body, the active interface being provided with valve and connecting with fermentor tank leakage fluid dram outside tank body.The opposite side top relative with fluid inlet at basin is provided with inlet mouth, inlet mouth has pipeline to lead to the exhaust endless tube made close to tank base place pipeline in tank body, the circular hole of 4-8 diameter 3-6mm is had to gas transmission in tank, interface valve being housed outside tank body and connecting with air filter above exhaust endless tube.Be provided with discharge outlet in the bottom center of basin, discharge outlet is provided with valve, run in the mouth of a river in parallel connection and with the bacterial classification delivery port of inoculator catheter loose joint.Described cover is provided with handheld ring, tensimeter, vent valve, band lamp window, has seal washer between cover and tank body, and fixing by screw activity.
Described air filter is fixed on the inlet mouth side of basin, air filter is two stage membrane air filter, filter sizes is less than 1 micron, the various microorganism of energy filtering, ability 120 DEG C of high pressure steam sterilizations, filtering traffic should be more than or equal to 80 liters/min, and the inlet end of air filter connects with the aspirating end of air compressor machine through flexible pipe, and exhaust side connects with the interface of inlet mouth.
Described inoculator is injection bowl inoculator, connects through the interface of flexible pipe with bacterial classification delivery port.
Described air compressor machine is 220V small-sized air compressor.
The theoretical foundation of the present invention's design: (1) 2003 year Shenyang academy of agricultural sciences, Shenyang Normal University Zhao Hong newly etc. proves with the preservation test that mushroom liquid bacterial does, mushroom liquid bacterial is shaking in the complete triangular flask of training, and preservation used after 40 days trains complete result of use immediately without too big-difference with shaking.In its test, bacterium liquid timing every day in triangular flask is shaken 30 minutes on 110r/min shaking table, and cultured continuously 14 days, obvious every day has 23.5 hours, and bacterium liquid remains static.In bacterium liquid fermenting process, as long as close the vent valve on fermentor tank, keep tank pressure less than 0, even if having a power failure 10 hours, mushroom liquid bacterial still can continue to produce.Production experience is sufficient proof also, no matter liquid spawn is during the fermentation or after having fermented, under envrionment temperature below growth optimum temperuture, when stuffiness or trace ventilation, go through 10 hours or liquid spawn quality can not be affected for more time.Obviously be difficult to transport problem, not at bacterial classification itself, and transportation means and transporting method improper.(2) liquid spawn production process, from the expansion of test tube strains, shakes training and terminates to ferment tank, last about 22-23 days, only have object bacterial classification in ramp in this time period.Accomplish this point, have a set of tight pollution prevention technology measure to be crucial.(3) to by liquid spawn long distance transportation, first before liquid spawn packing, sterilising treatment will be carried out to liquid spawn container, its secondary guarantee liquid spawn is pollution-free when loading container and exporting from container, finally, also be the most key factor, namely at point process of assembling with will ensure in transit to be in positive pressure environment in tank, if without malleation in container, have to negative pressure-pumping method when exporting bacterial classification in point of destination, result gets how many bacterial classifications, enter how many air, the miscellaneous bacteria in device outer air, spore just enter container, contaminated bacteria liquid.
Using method of the present invention
(1) sterilizing and the infusion fluid bacterial classification of tank is inoculated in transport: first by clean for transport inoculation tank internal washing, water purification inputs from feed liquor body mouth, sewage is discharged from water port, again fluid inlet is connected with fermentor tank leakage fluid dram, bacterial classification delivery port connects with inoculator, air filter connects with the flexible pipe of air compressor machine, and wraps inoculator injection port with multilayer bundle; Then the valve of fluid inlet, vent valve, inlet mouth, discharge outlet, bacterial classification delivery port, water port is opened, disinfecting action by fermentor tank leakage fluid dram place inputs steam to tank body.Now, pressure gauge presses off and begins to rise, and the other end of the flexible pipe of vent valve, water port, air compressor machine and the injection port gauze of inoculator have steam to emerge, and the size of adjustment vent valve, inlet mouth, water port, bacterial classification delivery port controls the spillage of steam.When tensimeter gauge pressure will rise until 1kg/ square centimeter gradually, start timing, keep after 30 minutes, close the valve of water port and bacterial classification delivery port, steam off input valve, pressure gauge presses off and begins to decline, rapidly the flexible pipe of air compressor machine and air compressor machine are linked together, and open air compressor machine, now air enters transport inoculation tank by air filter, adjustment air turnover amount, tank pressure is kept to be stabilized in 0.1kg/ square centimeter, when tank temperature drop is to less than 25 DEG C, open fermentor tank leakage fluid dram, to transport inoculation filling liquid body bacterial classification, close inlet mouth, adjustment vent valve, bacterium liquid is inputted unobstructed, and keep tank pressure 0.1kg/ square centimeter.When observing bacterium flow container injection rate to the degree needed from band lamp window, close vent valve, fluid inlet and fermentor tank leakage fluid dram, disconnect fermentor tank leakage fluid dram and inoculate connecting of tank with transport.Meanwhile, open air compressor machine, open inlet mouth, allow air enter transport inoculation tank through air filter, adjustment vent valve, when tank pressure satisfies the demand pressure, (should higher than 0.1kg/ square centimeter) closes air compressor machine and inoculator valve.
(2) transport: as long as tank pressure does not drop to below 0.1kg/ square centimeter in transit, the generally range of driving within 4 hours, without the need to being transfer cask ventilation.When transport was more than 4 hours ranges of driving, then 4 hours of should being often separated by, open air compressor machine, open inlet mouth and vent valve, in tank, inject air 30 minutes, keep tank pressure at 0.1-0.2kg/ square centimeter, it is defeated then to close the follow-up reforwarding of air compressor machine, inlet mouth and vent valve.
(3) inoculate: after bacterial classification transports to point of destination, transfer room is ready, inoculator is introduced indoor, open air compressor machine, adjustment inlet mouth and vent valve, ventilate in tank, tolerance more than 50 liters/min, ensures that inoculation whole process tank pressure is greater than 0.1kg/ square centimeter, stirs bacterium liquid by air-flow, do not make bacterial strain deposit, ensure that the Peloton density exporting bacterium liquid is stablized.Open discharge outlet and bacterial classification delivery port valve, by the injection terminal spine of inoculator in material, opening inoculator switch, is that power extrudes bacterial classification with tank pressure.After extracting dispensing end, sealing punctures mouth rapidly.Whole process action is little, bacterial classification and the culture material ingress of air time short, therefore cause pollution there is very little risk because of operation.
Beneficial effect of the present invention
(1) adopt invention, the very large of liquid-spawn inoculation range expansion can be made.Build the liquid spawn factory that a stdn is large-scale, the bacterial classification demand in supply county or contiguous several county can be met, thus greatly reduce the investment of liquid culture production equipment, improve utilization ratio and the returns of investment of equipment.
(2) the present invention is adopted, liquid-spawn inoculation success ratio is high, speed is fast, for the Production Time can saving female kind, original seed, cultivar at present on a small scale Edible Fungi plantation family, thus greatly shorten the edible fungus culturing cycle, save the equipment of making bacterial classification, manpower, fuel, place, achieve energy-saving and emission-reduction, change the technical process of Edible Fungi, plantation family year plantation number of times increases, and turnover of funds is accelerated, and improves economic benefit greatly.
(3) adopt invention, liquid spawn produces and separates with cultivation, is beneficial to Edible Fungi to batch production, large-scale development.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
In figure: 1, basin, 2, air filter, 3, inoculator, 4, air compressor machine, 5, tank body, 6, cover, 7, tank pin 8, fluid inlet, 9, inlet mouth, 10, exhaust endless tube, 11, discharge outlet, 12, water port, 13, bacterial classification delivery port, 14, handheld ring, 15, tensimeter, 16, vent valve, 17, lamp window is with, 18, screw.
Embodiment
Below in conjunction with accompanying drawing, the present invention is further illustrated.
As shown in Figure 1, edible fungi liquid strain transport inoculation tank of the present invention is made up of basin 1, air filter 2, inoculator 3 and air compressor machine 4.Wherein basin 1 comprises tank body 5, cover 6 and tank pin 7, wherein tank body 5 for bottom be circular arc, top is provided with the cylinder of flange connector, diameter 400-500mm, high 700-1000mm; Cover 6 is for being provided with the round end socket shape of flange connector, tank pin 7 is welded into by 50mm angle steel, be welded on the base portion of tank body 5, make the vertical height 250-300mm with ground bottom basin 1, three tank pin 7 spacing 120 degree, in transport, tank pin 7 is fixed on transportation means or weight, ensures that in transportation, basin 1 does not fall.The flange hem width 40-50mm of tank body 5 and cover 6, thick 8-10mm, flange periphery equidistantly has 6-10 diameter 12-15mm screw, has seal washer between flange, fixing by screw 18 activity.Be provided with fluid inlet 8 in the upper end of basin 1 side, apart from tank body 5 flange 80-100mm, fluid inlet 8 has pipeline to lead to close to bottom tank body 5 in basin 1, the active interface being provided with valve and connecting with fermentor tank leakage fluid dram outside tank body; The opposite side top relative with fluid inlet 8 at basin 1 is provided with inlet mouth 9, same apart from tank body 5 flange 80-100mm, inlet mouth 9 has pipeline to lead to apart from discharge outlet (11) 40-60mm place tortuous one-tenth air exhaust loop pipe 10 in tank body, the circular hole of 4-8 diameter 3-6mm is had to gas transmission in tank, interface valve being housed outside tank body and connecting with air filter (2) above exhaust endless tube 10; Discharge outlet (11) is provided with in the bottom center of basin (1), discharge outlet (11) is provided with valve, run in the mouth of a river in parallel connection (12) and with the bacterial classification delivery port (13) of inoculator (3) catheter loose joint, the discharge of water of condensation and cleaning sullage when controlling discharge and the tank body autoclaving of bacterium liquid.Be provided with the handheld ring 14 facilitating basin 1 movement at cover 6, be provided with tensimeter 15 and vent valve 16 and be with lamp window 17.
Air filter 2 is fixed in the middle part of the tank body of inlet mouth 9 side of basin 1, air filter 2 is two stage membrane air filter, filter sizes is less than 1 micron, the various microorganism of energy filtering, ability 120 DEG C of high pressure steam sterilizations, filtering traffic should be more than or equal to 80 liters/min, and the inlet end of air filter 2 connects through the aspirating end of flexible pipe with air compressor machine 4, and exhaust side connects with the interface of inlet mouth 9.
Inoculator 3 is injection bowl inoculator, connects through the interface of flexible pipe with bacterial classification delivery port 13, relies on malleation output liquid bacterial classification in basin 1.
Air compressor machine 4 is 220V small-sized air compressor, and one answers accessory various.
The flexible pipe that inoculator 3 connects with air compressor machine 4 is air engine pipe special.
Other flexible pipes are Edible Fungi proprietary material.
Claims (1)
1. an edible fungi liquid strain transport inoculation tank, be made up of basin (1), air filter (2), inoculator (3) and air compressor machine (4), described air filter (2) is fixed on inlet mouth (9) side of basin (1), air filter (2) is two stage membrane air filter, its inlet end connects through the aspirating end of flexible pipe with air compressor machine (4), and exhaust side connects with the interface of inlet mouth (9), described inoculator (3) is injection bowl inoculator, connects through the interface of flexible pipe with bacterial classification delivery port (13), it is characterized in that, described basin (1) by bottom be circular arc, top is provided with the tank body (5) of flange connector, the round end socket shape cover (6) being provided with flange connector, the tank pin (7) that is fixed on tank body (5) bottom are formed, described cover (6) is provided with handheld ring (14), tensimeter (15), vent valve (16), band lamp window (17), the top of described tank body (5) side is provided with fluid inlet (8), fluid inlet (8) has pipeline through to close to pot bottom in tank body (5), the active interface being provided with valve and connecting with fermentor tank leakage fluid dram outside tank body (5), inlet mouth (9) is provided with on the opposite side top that tank body (5) is relative with fluid inlet (8), inlet mouth (9) has pipeline to lead to the exhaust endless tube (10) made close to tank body (5) bottom place pipeline in tank body (5), the circular hole of 4-8 diameter 3-6mm is had to gas transmission in tank in the top of exhaust endless tube (10), interface valve being housed outside tank body (5) and connecting with air filter (2), the bottom center of tank body (5) is provided with discharge outlet (11), discharge outlet (11) is provided with valve, parallel connection is run in the mouth of a river (12) and the bacterial classification delivery port (13) with inoculator (3) catheter loose joint, seal washer is had between tank body (5) and cover (6), and fixing by screw (18) activity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410218750.6A CN104017723B (en) | 2014-05-23 | 2014-05-23 | Edible fungi liquid strain transport inoculation tank |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410218750.6A CN104017723B (en) | 2014-05-23 | 2014-05-23 | Edible fungi liquid strain transport inoculation tank |
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| Publication Number | Publication Date |
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| CN104017723A CN104017723A (en) | 2014-09-03 |
| CN104017723B true CN104017723B (en) | 2015-08-19 |
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| CN201410218750.6A Expired - Fee Related CN104017723B (en) | 2014-05-23 | 2014-05-23 | Edible fungi liquid strain transport inoculation tank |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104604527B (en) * | 2015-02-11 | 2017-11-21 | 北京华绿生物科技有限公司 | A kind of asparagus aerobic fementation system of outside control pressure and temperature |
| CN104756868B (en) * | 2015-04-08 | 2017-04-05 | 浙江大学 | Herba Dendrobii inoculation device and inoculation method |
| CN104737821A (en) * | 2015-04-22 | 2015-07-01 | 荆门市仙居乡廖梅子香菇种植专业合作社 | Opening type movable inoculating process for edible mushrooms |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2672072Y (en) * | 2004-01-20 | 2005-01-19 | 李勇 | Jet gas lifting edible mushroom liquid strain ferment cultivation machine |
| CN201127201Y (en) * | 2007-12-13 | 2008-10-08 | 浙江大学 | Steam type edible fungus inoculator |
| CN201403350Y (en) * | 2009-04-01 | 2010-02-17 | 汪梦筱 | Small-sized cultivating pot for producing mushroom species in liquid |
| CN102267590A (en) * | 2011-04-29 | 2011-12-07 | 谢日禄 | Edible fungi liquid strain packing bag device and application method thereof |
| CN203855579U (en) * | 2014-05-23 | 2014-10-01 | 山西省农业科学院食用菌研究所 | Edible mushroom liquid strain conveying inoculation tank |
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Inventor after: Guo Shang Inventor after: Nan Xiaojie Inventor after: Wu Guoding Inventor after: Wang Hua Inventor after: Zhou Lin Inventor after: Ling Liang Inventor after: Guo Minghui Inventor after: Zhao Zhaolin Inventor after: Liu Xin Inventor after: Xiao Jinchuan Inventor after: Zhang Honggang Inventor before: Guo Shang Inventor before: Wang Hua Inventor before: Wu Guoding Inventor before: Liu Xin Inventor before: Xiao Jinchuan |
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Granted publication date: 20150819 Termination date: 20200523 |
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