CN104017723A - Transportation and inoculation pot of edible mushroom liquid strain - Google Patents
Transportation and inoculation pot of edible mushroom liquid strain Download PDFInfo
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- CN104017723A CN104017723A CN201410218750.6A CN201410218750A CN104017723A CN 104017723 A CN104017723 A CN 104017723A CN 201410218750 A CN201410218750 A CN 201410218750A CN 104017723 A CN104017723 A CN 104017723A
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- tank
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- air filter
- basin
- inoculator
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
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Abstract
The invention discloses a transportation and inoculation pot of an edible mushroom liquid strain and is composed of a storage tank, an air filter inoculator and an air compressor. The storage tank is composed of a tank body, a tank cover and tank feet. The storage tank is provided with a liquid inlet, an air inlet, an exhaust circular tube, a discharge opening, a drainage opening and a strain output opening. The tank cover is provided with a handle ring, a pressure gauge, an exhaust valve and an observation hole with a lamp. A seal washer is disposed between the tank cover and the tank body and is fixed by screws; the air filter can filter various microbes; the air inlet end of the air filter is connected to an air supply end of the air compressor; an exhaust end of the air filter is connected to an interface of the air inlet of the storage tank; and the inoculator is a pressure spraying type inoculator and is connected to an interface of the strain output opening of the storage tank. By adopting the transportation and inoculation pot, the inoculation range of the liquid strain can be expanded largely; investment for liquid strain production equipment can be reduced greatly; a process flow for edible mushroom production of small-size edible mushroom production growers is changed; a cultivation period of the edible mushrooms is greatly shortened; and economic benefits is greatly increased.
Description
Technical field
The present invention relates to technical field of edible fungi production, particularly for the device of edible fungi liquid strain packed and transported.
Background technology
At present, edible fungi liquid strain is comparatively advanced both at home and abroad edible fungus species production technology, there is energy-saving and emission-reduction, environmental protection, efficient and convenient, success ratio is high, therefore, at home and abroad be applied to a certain extent, but its application generally all adopts and now do the mode that existing use, scene are done on-the-spot use, popularity rate is also not bery high, its major cause is: (1) facility investment is larger, has certain technical difficulty; (2) liquid spawn producing is difficult to the transportation of guaranteeing the quality at a distance.The problem that prior art exists causes the application of liquid spawn to be confined to possess the scientific experiment chamber of good condition or the manufacturing enterprise of large edible bacterium batch production, and is difficult to be widely applied in most Edible Fungi person and middle-size and small-size Edible Fungi enterprise.
Can edible fungi liquid strain long distance transportation, use, has three key issues to need to solve, and the one, liquid spawn, under normal temperature or temperature control condition, extends guaranteeing the quality staging life of liquid spawn; The 2nd, adopt safely, be suitable for the edible fungi liquid strain container of vehicle long distance transportation; The 3rd, liquid spawn container facilitates the input and output of liquid spawn.CN102267590A discloses a kind of edible fungi liquid strain packing bag device and application method thereof, this invention is by adopting a kind of method for packaging plastic bags to pack liquid spawn, solved liquid spawn from centralized production to disperseing to use packing, transportation, the storage problem existing two links, there is the advantages such as packaging operation is easy, cost of investment is lower, but, container with plastics bag as liquid spawn storage and transport, is easy to damage, and is easy to pollution while inoculate in point of destination.
Summary of the invention
The object of the invention is the deficiency existing for prior art, a kind of edible fungi liquid strain transportation inoculation tank is provided, this transportation inoculation tank can be under normal temperature or temperature control condition, extend the quality guaranteed period of liquid spawn, be suitable for long-time, remote vehicle transportation, facilitate the input and output of liquid spawn, during operation, be difficult for polluting.
For achieving the above object, the present invention adopts following technical scheme.
Edible fungi liquid strain transportation inoculation tank consists of basin, air filter inoculator and air compressor machine.
Described basin is that circular arc, the top tank body, the round end socket shape cover that is provided with flange connector that are provided with flange connector, the tank pin that is fixed on basin bottom form by bottom.Top in basin one side is provided with fluid inlet, and fluid inlet has pipeline through to approaching pot bottom in tank body, the active interface that valve is installed outward and connects with fermentor tank leakage fluid dram at tank body.The opposite side top relative with fluid inlet at basin is provided with inlet mouth, inlet mouth has pipeline to lead to approach the exhaust endless tube that tank base place pipeline is made in tank body, the circular hole that has 4-8 diameter 3-6mm above exhaust endless tube is to gas transmission in tank, the interface that valve is housed outward and connects with air filter at tank body.Bottom center at basin is provided with discharge outlet, discharge outlet be provided with valve, parallel connection run in the mouth of a river and with the bacterial classification delivery port of inoculator catheter loose joint.Described cover is provided with handheld ring, tensimeter, vent valve, band lamp window, between cover and tank body, has seal washer, and movable fixing by screw.
Described air filter is fixed on inlet mouth one side of basin, air filter is two stage membrane air filter, filter membrane aperture is less than 1 micron, the various microorganisms of energy filtering, 120 ℃ of high pressure steam sterilizations of ability, filtering traffic should be more than or equal to 80 liters/min, and the inlet end of air filter connects with the aspirating end of air compressor machine through flexible pipe, and exhaust side connects with the interface of inlet mouth.
Described inoculator is injection bowl inoculator, through flexible pipe, connects with the interface of bacterial classification delivery port.
Described air compressor machine is 220V small-sized air compressor.
The theoretical foundation of the present invention's design: (1) 2003 year academy of agricultural sciences, Shenyang, the Zhao Hong of Shenyang Normal University preservation evidence that newly etc. use mushroom liquid bacterial is done, mushroom liquid bacterial is in shaking the complete triangular flask of training, and preservation is used after 40 days and shaken the complete result of use immediately of training without big-difference too.In its test, the bacterium liquid in triangular flask regularly shakes 30 minutes every day on 110r/min shaking table, and cultured continuously 14 days, obviously has 23.5 hours every day, and bacterium liquid remains static.In bacterium liquid fermenting process, as long as close the vent valve on fermentor tank, keep tank pressure less than 0, even if having a power failure 10 hours, mushroom liquid bacterial still can continue to produce.Production experience also fully proves, no matter liquid spawn is during the fermentation or after having fermented, under envrionment temperature below growth optimum temperuture, stuffiness or when ventilation trace, go through 10 hours or be can not affect liquid spawn quality for more time.Obviously the problem that is difficult to transportation, not at bacterial classification itself, and improper at transportation means and transporting method.(2) liquid spawn production process, from the expansion of test tube strains, shake training and finish to ferment tank, lasts about 22-23 days, in this time period, only has object bacterial classification in ramp.Accomplish this point, it is crucial having a set of tight pollution prevention technology measure.(3) if will be by liquid spawn long distance transportation, first to before liquid spawn packing, to liquid spawn container, carry out sterilising treatment, its less important assurance liquid spawn is pollution-free when packing container into and exporting from container, finally, also be the most key factor, dividing process of assembling and will guarantee in tank in positive pressure environment in transit, if container is interior without malleation, when point of destination output bacterial classification, have to negative pressure-pumping method, result is to get how many bacterial classifications, enter how many air, the miscellaneous bacteria in device outer air, spore just enter container, contaminated bacteria liquid.
Using method of the present invention
(1) sterilizing and the infusion fluid bacterial classification of transportation inoculation tank: first will transport inoculation tank internal washing clean, water purification is inputted from feed liquor body mouth, sewage is discharged from water port, again fluid inlet is connected with fermentor tank leakage fluid dram, bacterial classification delivery port connects with inoculator, air filter connects with the flexible pipe of air compressor machine, and wraps inoculator injection port with multilayer bundle; Then open the valve of fluid inlet, vent valve, inlet mouth, discharge outlet, bacterial classification delivery port, water port, by fermentor tank leakage fluid dram place, to tank body input steam, start disinfecting action.Now, pressure gauge presses off and begin to rise, and on the other end of flexible pipe of vent valve, water port, air compressor machine and the injection port gauze of inoculator, has steam to emerge, and the size of adjusting vent valve, inlet mouth, water port, bacterial classification delivery port is controlled the spillage of steam.When tensimeter gauge pressure will rise gradually until 1kg/ square centimeter, start timing, keep after 30 minutes, close the valve of water port and bacterial classification delivery port, steam off input valve, pressure gauge presses off and begins to decline, rapidly the flexible pipe of air compressor machine and air compressor machine are linked together, and open air compressor machine, now air enters transportation inoculation tank by air filter, adjust air turnover amount, keep tank pressure to be stabilized in 0.1kg/ square centimeter, in the time of below tank temperature drop to 25 ℃, open fermentor tank leakage fluid dram, to transportation inoculation filling liquid body bacterial classification, close inlet mouth, adjust vent valve, make the input of bacterium liquid unobstructed, and keep tank pressure 0.1kg/ square centimeter.From with lamp window, observe bacterium flow container injection rate to need degree time, close vent valve, fluid inlet and fermentor tank leakage fluid dram, disconnect fermentor tank leakage fluid dram and inoculate connecting of tank with transportation.Meanwhile, open air compressor machine, open inlet mouth, allow air enter transportation inoculation tank through air filter, adjust vent valve, when tank pressure is satisfied the demand pressure, (should higher than 0.1kg/ square centimeter) closes air compressor machine and inoculator valve.
(2) transportation: in transit as long as tank pressure does not drop to below 0.1kg/ square centimeter, generally at 4 hours with the interior range of driving, without ventilating for transfer cask.When transportation surpasses 4 hours ranges of driving, should often be separated by about 4 hours, open air compressor machine, open inlet mouth and vent valve, in tank, inject air 30 minutes, keep tank pressure at 0.1-0.2kg/ square centimeter, then close the follow-up reforwarding of air compressor machine, inlet mouth and vent valve defeated.
(3) inoculation: bacterial classification is transported to behind point of destination, transfer room is ready, inoculator introducing is indoor, open air compressor machine, adjust inlet mouth and vent valve, in tank, ventilate, tolerance more than 50 liters/min, guarantees that inoculation whole process tank pressure is greater than 0.1kg/ square centimeter, stirs bacterium liquid by air-flow, do not make bacterial strain deposition, guarantee that the Peloton density of output bacterium liquid is stable.Open discharge outlet and bacterial classification delivery port valve, the injection terminal spine of inoculator, in material, is opened to inoculator switch, take tank pressure as power extrusion bacterial classification.Extract after dispensing end the cut of sealing thorn rapidly.Whole process action is little, and bacterial classification and culture material ingress of air time are short, therefore there is very little risk because operation causes pollution.
Beneficial effect of the present invention
(1) adopt invention, can make the very large of liquid-spawn inoculation range expansion.Build a liquid spawn factory that stdn is large-scale, can meet the bacterial classification demand in supply Yi Ge county or contiguous several counties, thereby greatly reduce the investment of liquid culture production equipment, improve utilization ratio and the returns of investment of equipment.
(2) adopt the present invention, liquid-spawn inoculation success ratio is high, speed is fast, for current small-scale Edible Fungi plantation family, can save female kind, Production Time of original seed, cultivar, thereby greatly shorten the edible fungus culturing cycle, saved the equipment, manpower, fuel, the place that make bacterial classification, realized energy-saving and emission-reduction, changed the technical process of Edible Fungi, plantation family year plantation number of times increases, and turnover of funds is accelerated, and has improved greatly economic benefit.
(3) adopt invention, liquid spawn produces with cultivation and separates, and is beneficial to Edible Fungi to batch production, large-scale development.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
In figure: 1, basin, 2, air filter, 3, inoculator, 4, air compressor machine, 5, tank body, 6, cover, 7, tank pin 8, fluid inlet, 9, inlet mouth, 10, exhaust endless tube, 11, discharge outlet, 12, water port, 13, bacterial classification delivery port, 14, handheld ring, 15, tensimeter, 16, vent valve, 17, band lamp window, 18, screw.
Embodiment
Below in conjunction with accompanying drawing, the present invention is further illustrated.
As shown in Figure 1, edible fungi liquid strain transportation inoculation tank of the present invention consists of basin 1, air filter 2, inoculator 3 and air compressor machine 4.Wherein basin 1 comprises tank body 5, cover 6 and tank pin 7, and wherein tank body 5 is the cylinder that circular arc, top are provided with flange connector, diameter 400-500mm, high 700-1000mm for bottom; Cover 6 is for being provided with the round end socket shape of flange connector, tank pin 7 is welded into by 50mm angle steel, be welded on the base portion of tank body 5, make the vertical height 250-300mm on basin 1 bottom and ground, three tank pin 7 spacing 120 degree, in transportation, tank pin 7 is fixed on transportation means or weight, guarantees that in transportation, basin 1 does not fall.The flange hem width 40-50mm of tank body 5 and cover 6, thick 8-10mm, flange periphery equidistantly has 6-10 diameter 12-15mm screw, has seal washer between flange, movable fixing by screw 18.Upper end in basin 1 one sides is provided with fluid inlet 8, and apart from tank body 5 flange 80-100mm, fluid inlet 8 has pipeline to lead to approach tank body 5 bottoms, the active interface that valve is installed and connects with fermentor tank leakage fluid dram at tank body outward in basin 1; The opposite side top relative with fluid inlet 8 at basin 1 is provided with inlet mouth 9, equally apart from tank body 5 flange 80-100mm, inlet mouth 9 has pipeline to lead to apart from discharge outlet (11) 40-60mm place tortuous one-tenth air exhaust loop pipe 10 in tank body, the circular hole that has 4-8 diameter 3-6mm above exhaust endless tube 10 is to gas transmission in tank, the interface that valve is housed outward and connects with air filter (2) at tank body; Bottom center at basin (1) is provided with discharge outlet (11), discharge outlet (11) be provided with valve, parallel connection run in the mouth of a river (12) and with the bacterial classification delivery port (13) of inoculator (3) catheter loose joint, the discharge of water of condensation and cleaning sullage while controlling the discharge of bacterium liquid and tank body autoclaving.At cover 6, be provided with the handheld ring 14 that facilitates basin 1 to move, be provided with tensimeter 15 and vent valve 16 and band lamp window 17.
Air filter 2 is fixed on the tank body middle part of inlet mouth 9 one sides of basin 1, air filter 2 is two stage membrane air filter, filter membrane aperture is less than 1 micron, the various microorganisms of energy filtering, 120 ℃ of high pressure steam sterilizations of ability, filtering traffic should be more than or equal to 80 liters/min, and the inlet end of air filter 2 connects with the aspirating end of air compressor machine 4 through flexible pipe, and exhaust side connects with the interface of inlet mouth 9.
Inoculator 3 is injection bowl inoculator, through flexible pipe, connects with the interface of bacterial classification delivery port 13, relies on the interior malleation output liquid of basin 1 bacterial classification.
Air compressor machine 4 is 220V small-sized air compressor, and one answers accessory various.
The flexible pipe that inoculator 3 connects with air compressor machine 4 is air engine pipe special.
Other flexible pipes are Edible Fungi proprietary material.
Claims (4)
1. an edible fungi liquid strain transportation inoculation tank, is characterized in that, edible fungi liquid strain transportation inoculation tank consists of basin (1), air filter (2) inoculator (3) and air compressor machine (4).
2. tank is inoculated in edible fungi liquid strain transportation according to claim 1, it is characterized in that, described basin (1) is circular arc by bottom, top is provided with the tank body (5) of flange connector, be provided with the round end socket shape cover (6) of flange connector, the tank pin (7) that is fixed on basin (1) bottom forms, described cover (6) is provided with handheld ring (14), tensimeter (15), vent valve (16), band lamp window (17), between cover (6) and tank body (5), there is seal washer, and movable fixing by screw (18), it is characterized in that, on the top of basin (1) one side, be provided with fluid inlet (8), fluid inlet (8) has pipeline through to approaching pot bottom in tank body, the active interface that valve is installed outward and connects with fermentor tank leakage fluid dram at tank body, the opposite side top relative with fluid inlet (8) at basin (1) is provided with inlet mouth (9), the exhaust endless tube (10) that inlet mouth (9) has pipeline to lead to approach place, tank body (5) bottom pipeline to make in tank body, the circular hole that has a 4-8 diameter 3-6mm in the top of exhaust endless tube (10) is to gas transmission in tank, the interface that valve is housed outward and connects with air filter (2) at tank body, bottom center at basin (1) is provided with discharge outlet (11), discharge outlet (11) be provided with valve, parallel connection run in the mouth of a river (12) and with the bacterial classification delivery port (13) of inoculator (3) catheter loose joint.
3. tank is inoculated in edible fungi liquid strain transportation according to claim 1, it is characterized in that, described air filter (2) is fixed on inlet mouth (9) one sides of basin (1), it is characterized in that, air filter (2) is two stage membrane air filter, filter membrane aperture is less than 1 micron, the various microorganisms of energy filtering, 120 ℃ of high pressure steam sterilizations of ability, filtering traffic should be more than or equal to 80 liters/min, the inlet end of air filter (2) connects with the aspirating end of air compressor machine (4) through flexible pipe, and exhaust side connects with the interface of inlet mouth (9).
4. edible fungi liquid strain transportation inoculation tank according to claim 1, is characterized in that, described inoculator (3) is injection bowl inoculator, through flexible pipe, connects with the interface of bacterial classification delivery port (13).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410218750.6A CN104017723B (en) | 2014-05-23 | 2014-05-23 | Edible fungi liquid strain transport inoculation tank |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410218750.6A CN104017723B (en) | 2014-05-23 | 2014-05-23 | Edible fungi liquid strain transport inoculation tank |
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| Publication Number | Publication Date |
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| CN104017723A true CN104017723A (en) | 2014-09-03 |
| CN104017723B CN104017723B (en) | 2015-08-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410218750.6A Expired - Fee Related CN104017723B (en) | 2014-05-23 | 2014-05-23 | Edible fungi liquid strain transport inoculation tank |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104604527A (en) * | 2015-02-11 | 2015-05-13 | 北京华绿生物科技有限公司 | Needle mushroom aerated fermentation system with external pressure and temperature control functions |
| CN104737821A (en) * | 2015-04-22 | 2015-07-01 | 荆门市仙居乡廖梅子香菇种植专业合作社 | Opening type movable inoculating process for edible mushrooms |
| CN104756868A (en) * | 2015-04-08 | 2015-07-08 | 浙江大学 | Dendrobium candidum inoculation machine and inoculation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2672072Y (en) * | 2004-01-20 | 2005-01-19 | 李勇 | Jet gas lifting edible mushroom liquid strain ferment cultivation machine |
| CN201127201Y (en) * | 2007-12-13 | 2008-10-08 | 浙江大学 | Steam type edible fungus inoculator |
| CN201403350Y (en) * | 2009-04-01 | 2010-02-17 | 汪梦筱 | Small-sized cultivating pot for producing mushroom species in liquid |
| CN102267590A (en) * | 2011-04-29 | 2011-12-07 | 谢日禄 | Edible fungi liquid strain packing bag device and application method thereof |
| CN203855579U (en) * | 2014-05-23 | 2014-10-01 | 山西省农业科学院食用菌研究所 | Edible mushroom liquid strain conveying inoculation tank |
-
2014
- 2014-05-23 CN CN201410218750.6A patent/CN104017723B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2672072Y (en) * | 2004-01-20 | 2005-01-19 | 李勇 | Jet gas lifting edible mushroom liquid strain ferment cultivation machine |
| CN201127201Y (en) * | 2007-12-13 | 2008-10-08 | 浙江大学 | Steam type edible fungus inoculator |
| CN201403350Y (en) * | 2009-04-01 | 2010-02-17 | 汪梦筱 | Small-sized cultivating pot for producing mushroom species in liquid |
| CN102267590A (en) * | 2011-04-29 | 2011-12-07 | 谢日禄 | Edible fungi liquid strain packing bag device and application method thereof |
| CN203855579U (en) * | 2014-05-23 | 2014-10-01 | 山西省农业科学院食用菌研究所 | Edible mushroom liquid strain conveying inoculation tank |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104604527A (en) * | 2015-02-11 | 2015-05-13 | 北京华绿生物科技有限公司 | Needle mushroom aerated fermentation system with external pressure and temperature control functions |
| CN104756868A (en) * | 2015-04-08 | 2015-07-08 | 浙江大学 | Dendrobium candidum inoculation machine and inoculation method |
| CN104737821A (en) * | 2015-04-22 | 2015-07-01 | 荆门市仙居乡廖梅子香菇种植专业合作社 | Opening type movable inoculating process for edible mushrooms |
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| CN104017723B (en) | 2015-08-19 |
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Inventor after: Guo Shang Inventor after: Nan Xiaojie Inventor after: Wu Guoding Inventor after: Wang Hua Inventor after: Zhou Lin Inventor after: Ling Liang Inventor after: Guo Minghui Inventor after: Zhao Zhaolin Inventor after: Liu Xin Inventor after: Xiao Jinchuan Inventor after: Zhang Honggang Inventor before: Guo Shang Inventor before: Wang Hua Inventor before: Wu Guoding Inventor before: Liu Xin Inventor before: Xiao Jinchuan |
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Granted publication date: 20150819 Termination date: 20200523 |