CN102199528B - Device for sampling microorganisms from surface of object - Google Patents
Device for sampling microorganisms from surface of object Download PDFInfo
- Publication number
- CN102199528B CN102199528B CN 201110055883 CN201110055883A CN102199528B CN 102199528 B CN102199528 B CN 102199528B CN 201110055883 CN201110055883 CN 201110055883 CN 201110055883 A CN201110055883 A CN 201110055883A CN 102199528 B CN102199528 B CN 102199528B
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- CN
- China
- Prior art keywords
- culture dish
- class culture
- class
- inner core
- shell
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a device which can be used for sampling microorganisms from the surface of an object. The microorganisms are directly collected from the surface of the object and are cultured. The device consists of four parts, namely a shell, an inner core, a rotating component, and a class culture dish. A method comprises the following steps of: under aseptic conditions, opening a shell cover, uncovering a seal of the class culture dish, putting a bottom groove of the class culture dish into a microorganism culture tank of the device, making a culture medium extended out of the edge of a shell cylinder by rotating the rotating component, attaching the surface of the culture medium to the surface of an object to be detected, taking down after certain time, immediately covering the shell cover, sealing a gap between the shell cover and the shell cylinder by using a PARAFILM, inverting the microorganism sampler, culturing under appropriate conditions, and further detecting. The device is easy to operate, is convenient to clean, can directly culture microorganisms to be detected, and can be repeatedly used.
Description
Technical field
The present invention relates to a kind of device for sampling microorganisms from surface of object.This device can carry out the surface microorganism sampling easily, and directly carries out microorganism culturing, and microbe sampling and culturing process is integrated, improves the surface microorganism detection efficiency.In addition, this device is reusable, has not only saved but also environmental protection.
Background technology
Microorganism is ubiquitous.The existence of certain micro-organisms not only pollutes the environment, and harm people's health also can heavy corrosion and the multiple object of destruction.For reducing the harm of microorganism, need to sample, cultivate microorganism and further detect.Microbe sampling is determining the validity of follow-up work, and is particularly important.Microbe sampling relates to the every field of microbe research, comprises medical science, manned space flight etc.Wherein, the research of body surface microorganism is a very important aspect.Yet existing surface microorganism sampling mainly is by disinfecting cotton swab wiping surface to be detected, transfers in the culture dish and cultivates.The method is comparatively loaded down with trivial details, and cotton swab is sampled, transfer process may cause larger microorganism count error.
Therefore, how to utilize simpler, easy row, device improves the surface microorganism sampling efficiency accurately just becomes key.
Summary of the invention
For the problems referred to above, the present invention proposes a kind of device for sampling microorganisms from surface of object.This device is comprised of four parts: shell, inner core, turn parts, class culture dish.
Shell is made of column circular housing cylinder and the transparent outer cover lid that matches.Outer thimble and casing cover adopt high temperature resistant, corrosion-resistant hard organic synthesis material.Outer thimble height is 3～20cm, and internal diameter is 2～15cm; The casing cover height is 1～2cm, and internal diameter is greater than the outer thimble external diameter 0.1～0.2cm of edge.
Inner core is column, adopts high temperature resistant, corrosion-resistant hard organic synthesis material preparation.Its height is less than outer cover height 1.0～2.0cm, and external diameter is less than internal diameter of outer cover 0.1～0.2mm.The microorganism culturing groove of dark 0.5～1.0cm internal diameter, 1.6～14.5cm is established in the inner core upper end.Inner core links to each other by screw thread with the turn parts.
The turn parts couple together shell and inner core, and its bottom outer surface is concavo-convex striated, so that when increasing turn and the frictional force of hand.By rotating these turn parts inner core is moved up and down.
The class culture dish is made of class culture dish kerve and class culture dish sealing two portions.High 0.1～the 0.5cm of the degree of depth of the aspect ratio microorganism culturing groove of class culture dish kerve, external diameter is with microorganism culturing groove internal diameter.Class culture dish kerve adopts high temperature resistant, antibiotic, elasticity organic polymer material preparation.The sealing of class culture dish is one deck antibacterial plastic film, tightly is covered in the mouth of class culture dish kerve by hot pressing, prevents that microorganism from entering.Such culture dish is disposable loss product, under aseptic condition, in class culture dish kerve, inject aseptic culture medium, class culture dish sealed membrane tightly is covered in the mouth of class culture dish kerve in the mode of hot pressing, with the plastic packing box sterile packed that comprises some grooves, and for subsequent use with the tinfoil sealed groove.
Description of drawings
Fig. 1 is the SURFACE MICROBIOLOGICAL SAMPLER sectional view of apparatus of the present invention.
Fig. 2 is class culture dish (band packing) sectional view of apparatus of the present invention.
Among the figure: the 1st, casing cover; The 2nd, outer thimble is with casing cover 1 common formation shell, as shown in Figure 1; The 3rd, turn parts, connected with outer casing cylinder and inner core; The 4th, inner core; The 5th, the microorganism culturing groove; The 6th, class culture dish kerve; The 7th, the sealing of class culture dish; The 8th, plastic packing box; The 9th, tinfoil, sealing class culture dish prevents from polluting when being used for packing; The 10th, groove.
Embodiment
The present invention will be further described below in conjunction with Figure of description.The present invention's scope required for protection is not limited to the scope described in the embodiment.
Before using this contrive equipment to carry out sampling microorganisms from surface of object, at first with alcohol the microorganism of this device surface sampling thief (such as accompanying drawing 1) is carried out pasteurised completely.After sterilization is finished, under aseptic technique, tinfoil 9 on the plastic packing box 8 opened (open one with one, avoid polluting), take out class culture dish (consisting of by 6 and 7) wherein, open the casing cover 1 of this SURFACE MICROBIOLOGICAL SAMPLER (such as accompanying drawing 1), open class culture dish sealing 7, such culture dish (consisting of by 6 and 7) is placed microorganism culturing groove 5, can carry out the surface microorganism sampling.
When using this contrive equipment sampling, at first by rotation turn parts 3 media surface is leant out more than the edge 0.2cm of outer thimble 2, attach the examining object surface (because class culture dish kerve 6 is resilient material with media surface, can guarantee that by its deformation substratum fully contacts with treating the sampling surface), take off behind the certain hour, make the substratum after the sampling be in outer thimble 2 inside fully by rotation turn parts 3, add a cover casing cover 1, by twine the sealing gap between casing cover 1 and the outer thimble 2 with the PARAFILM sealed membrane, sampling finishes.
After using this contrive equipment sampling to finish, the microorganism sampler (such as accompanying drawing 1) of this device is inverted, under suitable condition, is carried out microorganism culturing, and further detect.
Use this contrive equipment to carry out surface microorganism sampling and cultivate finish after, open casing cover 1, make microorganism culturing groove 5 lean out the edge of outer thimble 2 by rotation turn parts 3, the edge of held by both hands microorganism culturing groove 5 interior class culture dish kerves 6 takes out it, disposes as refuse.Simultaneously, the microorganism sampler (such as accompanying drawing 1) of this device is carried out sterilization and disinfection process, microorganism culturing groove 5 is screwed in the outer thimble 2, add a cover casing cover 1 for subsequent use.
Claims (2)
1. device for sampling microorganisms from surface of object, mainly consisted of by four parts, shell, inner core, turn parts and class culture dish, the turn parts connect into shell and inner core as a whole, and wherein, the turn parts twist together mutually with the protrusion of shell by the shell bottom, but the two can relatively rotate, simultaneously, the turn parts are connected with the Z-shaped screw thread of inner core by the inner core lower inside, and the class culture dish is packed separately;
The shell of this device is made of column circular housing cylinder and the transparent outer cover lid that matches; Outer thimble and casing cover adopt high temperature resistant, corrosion-resistant hard organic synthesis material, and outer thimble height is 3 ~ 20cm, and internal diameter is 2～15cm, and the casing cover height is 1 ~ 2cm, and internal diameter is greater than the outer thimble external diameter 0.1 ~ 0.2cm of edge;
The inner core of this device is column, adopts high temperature resistant, corrosion-resistant hard organic synthesis material preparation, and its height is less than outer cover height 1.0 ~ 2.0cm, and external diameter is less than internal diameter of outer cover 0.1 ~ 0.2mm; The microorganism culturing groove of dark 0.5 ~ 1.0cm internal diameter, 1.6 ~ 14.5cm is established in the inner core upper end, and inner core links to each other by screw thread with the turn parts;
The class culture dish of this device is made of class culture dish kerve and class culture dish sealing two portions; High 0.1 ~ the 0.5cm of the degree of depth of the aspect ratio microorganism culturing groove of class culture dish kerve, external diameter is with microorganism culturing groove internal diameter; Class culture dish kerve adopts high temperature resistant, antibiotic, elasticity organic polymer material preparation, and the sealing of class culture dish is one deck antibacterial plastic film, tightly is covered in the mouth of class culture dish kerve by hot pressing, prevents that microorganism from entering; Such culture dish is disposable loss product, under aseptic condition, in class culture dish kerve, inject aseptic culture medium, class culture dish sealed membrane tightly is covered in the mouth of class culture dish kerve in the mode of hot pressing, with the plastic packing box sterile packed that comprises some grooves, and for subsequent use with the tinfoil sealed groove.
2. install as claimed in claim 1, it is characterized in that, in use, open tinfoil and take out the class culture dish, after class culture dish sealed membrane is thrown off class culture dish kerve is put into the microorganism culturing groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110055883 CN102199528B (en) | 2011-03-08 | 2011-03-08 | Device for sampling microorganisms from surface of object |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110055883 CN102199528B (en) | 2011-03-08 | 2011-03-08 | Device for sampling microorganisms from surface of object |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102199528A CN102199528A (en) | 2011-09-28 |
| CN102199528B true CN102199528B (en) | 2013-03-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110055883 Expired - Fee Related CN102199528B (en) | 2011-03-08 | 2011-03-08 | Device for sampling microorganisms from surface of object |
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| Country | Link |
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| CN (1) | CN102199528B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102732419B (en) * | 2012-06-08 | 2013-11-13 | 北京航空航天大学 | Object surface microbe sampling culture dish |
| CN106635765A (en) * | 2016-11-07 | 2017-05-10 | 天津施特雷生物科技股份有限公司 | Disposable sterile microbial culture dish |
| CN111733204A (en) * | 2020-07-03 | 2020-10-02 | 安徽屾远材料技术有限公司 | Microbial rapid detection membrane and preparation method and use method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101245315A (en) * | 2008-03-13 | 2008-08-20 | 上海交通大学 | Cultivation device and method for adherent cell thin membrane of laboratory |
| CN101528912A (en) * | 2005-09-26 | 2009-09-09 | 快速微型生物系统公司 | Cassette containing growth medium |
| CN101659921A (en) * | 2009-09-02 | 2010-03-03 | 青岛众瑞智能仪器有限公司 | Full automatic microorganism sampler |
-
2011
- 2011-03-08 CN CN 201110055883 patent/CN102199528B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101528912A (en) * | 2005-09-26 | 2009-09-09 | 快速微型生物系统公司 | Cassette containing growth medium |
| CN101245315A (en) * | 2008-03-13 | 2008-08-20 | 上海交通大学 | Cultivation device and method for adherent cell thin membrane of laboratory |
| CN101659921A (en) * | 2009-09-02 | 2010-03-03 | 青岛众瑞智能仪器有限公司 | Full automatic microorganism sampler |
Non-Patent Citations (2)
| Title |
|---|
| 胡庆轩等.微孔滤膜空气微生物采样器的研制.《卫生研究》.1999,第28卷(第04期),255-256. * |
| 车凤翔.空气微生物采样法.《消毒与灭菌》.1987,第4卷(第03期),143-149. * |
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| Publication number | Publication date |
|---|---|
| CN102199528A (en) | 2011-09-28 |
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Granted publication date: 20130327 Termination date: 20140308 |