CN103421684B - A kind of aerogens quick test pipe - Google Patents

A kind of aerogens quick test pipe Download PDF

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Publication number
CN103421684B
CN103421684B CN201210155176.5A CN201210155176A CN103421684B CN 103421684 B CN103421684 B CN 103421684B CN 201210155176 A CN201210155176 A CN 201210155176A CN 103421684 B CN103421684 B CN 103421684B
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China
Prior art keywords
pipe
lucifuge
outer tube
capping
aerogens
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CN201210155176.5A
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CN103421684A (en
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陈功祥
伊藤春夫
詹亚男
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Hangzhou Genesis Biodetection & Biocontrol Ltd
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Hangzhou Genesis Biodetection & Biocontrol Ltd
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Abstract

The invention discloses a kind of aerogens quick test pipe, comprise the outer tube of lower end closed and be located at the capping at outer tube mouth of pipe place, described capping upper surface is to having the pipe connecting passed for a lucifuge pipe; The upper end closed of described lucifuge pipe, lower ending opening, the outer wall of lucifuge pipe coordinates with the inwall slidable sealing of pipe connecting; The lower end of pipe connecting is provided with a lucifuge plug, and this lucifuge plug slidably coordinates with pipe connecting; Be installed with the interior pipe of one end open in described outer tube, down, and gap is left in the bottom of opening end and outer tube to its opening end, leaves gap between the outer wall of interior pipe and the inwall of outer tube.This test tube can make the extended shelf-life of substratum, and meanwhile, cost of the present invention is lower, easy and simple to handle, and aerogenesis situation is observed accurately.

Description

A kind of aerogens quick test pipe
Technical field
The present invention relates to a kind of equipment detecting Institute of Micro-biology, especially relate to the test tube that a kind of aerogens is cultivated and inspection institute uses.
Background technology
Coliform means a group energy ferment lactose, produces sour aerogenesis, aerobic and amphimicrobian Gram-negative bactacin, be the monitoring of hygiene must micrometer one of biological.The units such as food and medicine Surveillance Authority, customs, commodity inspection, food enterprise and pharmaceutical factory often need to check coliform, and the current method of inspection has ordinary method and the Rapid examination method of national regulations.In the multiple fermentation test of ordinary method, to first prepare BGLG substratum, be dispensed in the test tube of Du Shi tubule, then culture is inoculated in this test tube cultivates, observe aerogenesis situation by Du Shi tubule.This method needs the complicated processes such as preparation substratum, packing and sterilizing, and most reviewer often to run into after sterilizing alveolate problem in Du Shi tubule, culture tube can not be used, both lost time and reagent, increase again the workload of reviewer.Rapid examination method mainly contains coliform fast detector and Quick detecting paper sheet for colibacillus group, although Rapid examination method required time is short, fast detector is expensive, and middle-size and small-size unit is difficult to configuration; The quick test scraps of paper can only be observed and produce sour situation, cannot observe aerogenesis situation.
In addition, the aerogenesis situation of some scientific research institutions microorganism such as type such as aerogenesis such as research clostridium perfringens and yeast etc., generally Du Shi tubule is inverted in test tube, observe the intratubular gas of Du Shi, and place the operating skill of Du Shi tubule to scientific research personnel and there are certain requirements, if it is improper to place, can makes there is bubble in Du Shi tubule, affect observations.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, provide a kind of aerogenesis situation of observing accurate, convenient, and aerogens quick test pipe simple to operate.
For achieving the above object, the present invention can take following technical proposals: a kind of aerogens quick test pipe, comprises the outer tube of lower end closed and is located at the capping at outer tube mouth of pipe place, and described capping upper surface is to having the pipe connecting passed for a lucifuge pipe; The upper end closed of described lucifuge pipe, lower ending opening, the outer wall of lucifuge pipe coordinates with the inwall slidable sealing of pipe connecting; The lower end of pipe connecting is provided with a lucifuge plug, and this lucifuge plug slidably coordinates with pipe connecting; Be installed with the interior pipe of one end open in described outer tube, down, and gap is left in the bottom of opening end and outer tube to its opening end, leaves gap between the outer wall of interior pipe and the inwall of outer tube.
As preferably, the outer wall of described lucifuge pipe is provided with flange collar, and lucifuge pipe is coordinated with pipe connecting inner wall sealing by this flange collar.
As preferably, the outer of described lucifuge pipe is arranged with an annular projection.
As preferably, the outer of described lucifuge pipe is arranged with a collar, and this collar is between annular projection and the upper surface of capping.
As preferably, the side of described capping is radially provided with flange, along flange inner to having press section.
As preferably, described interior pipe is by arranging union lever and outer tube is connected.
As preferably, described capping is located at the mouth of pipe place of outer tube by hinged mode.By hinged mode, conveniently to open, can one-handed performance be realized.
As preferably, described outer tube, interior pipe are lucite pipe.
As preferably, described lucifuge pipe, lucifuge plug are plastics tubing.
Aerogens quick test pipe of the present invention, upper end is provided with lucifuge pipe, lucifuge pipe is through capping, seal cartridge is in pipe connecting, can slide up and down in pipe connecting, be plugged with a lucifuge plug in pipe connecting lower end, this lucifuge plug and lucifuge pipe are opaque plastics tubing and make, and can deposit and see the labile composition of light.Also be slidably coordinate between lucifuge plug and pipe connecting, when pressing lucifuge pipe upper end, lucifuge plug will be ejected by lucifuge pipe, namely lucifuge plug splits away off from pipe connecting, material in such lucifuge pipe is just dropped in the substratum in outer tube, lucifuge plug also can fall into substratum, but lucifuge plug is through sterilising treatment, does not affect experiment.
Before experiment, first by seeing in substratum that the labile composition of light adds in lucifuge pipe, other compositions of substratum are added in outer tube, for subsequent use after sterilizing.During experiment, the upper end of pressing lucifuge pipe, the material in lucifuge pipe is dropped in outer tube, shakes up and can test.As: in the multiple fermentation test of inspection coliform, open capping, in advance bilein etc. can be shown in that the labile composition of light adds in lucifuge pipe, then lucifuge plug beyond the Great Wall, lucifuge pipe and lucifuge plug are pushed in pipe connecting; Again other compositions of BGLB substratum are added outer tube, cover capping, save backup after sterilizing.During use, take away collar, pressing lucifuge pipe, lucifuge plug is made to be ejected pipe connecting by lucifuge pipe, in lucifuge plug and lucifuge pipe, material falls into outer tube, can open capping on the other hand after shaking up, and adds on the other hand and treats that test sample is cultivated, observe bubble production in nutrient solution colour-change and interior pipe, judge to treat in test sample, whether have coliform to exist with this.
In prior art, general substratum needs matching while using, uses inconvenience, and cultivates test tube used and stopper separates, and easily pollutes, and opens also inconvenient.And capping of the present invention and body are opened conveniently, can realize one-handed performance, and add lucifuge composition in advance, during use, only need press lucifuge pipe can carry out test experiments, simple operation, decreases contaminated probability; Lucifuge pipe can be deposited in advance and see the labile composition of light, by the time just lucifuge material is dropped into substratum during experiment, and the mode taking substratum and lucifuge composition separately to preserve, makes the extended shelf-life of substratum.
Moreover, when observing microorganism aerogenesis situation in prior art, need to place in addition a tubule and observe, if place improper, can make there is bubble in tubule, affect observations, and interior pipe of the present invention and outer tube be structure as a whole, without the need to other placement, easy to operate, and observations is more accurate.
In sum, the present invention has the following advantages:
1. the present invention does not need proofer to prepare the set-up procedure such as substratum and sterilizing, directly can add and treat test sample quick test, convenient, greatly reduces contaminated probability;
2. lucifuge pipe of the present invention can be deposited in advance and see the labile composition of light, makes the extended shelf-life of substratum;
3. cost of the present invention is lower, easy and simple to handle, and aerogenesis situation is observed accurately.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Fig. 2 is open mode schematic diagram of the present invention.
Fig. 3 is end construction schematic diagram of the present invention.
Embodiment
Better the present invention program is understood in order to make those skilled in the art person, below in conjunction with the accompanying drawing in the embodiment of the present invention, clear, complete description is carried out to the technical scheme in the embodiment of the present invention, obviously, described embodiment is only a part of embodiment of the present invention, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, should belong to the scope of protection of the invention.
As Fig. 1, shown in Fig. 2, a kind of aerogens quick test pipe, comprise transparent plastics outer tube 1, the lucifuge pipe 5 that transparent plastics capping 3 and opaque plastics are made, the lower end closed of outer tube 1, upper end open, the side of capping 3 and the mouth of pipe of outer tube 1 hinged, hinged mode more conveniently can open capping, free side and the outer tube 1 of capping 3 fasten, capping 3 upper surface has a through hole, a pipe connecting 4 is extended downwards along this through hole, the upper end of this pipe connecting 4 and the end face of capping 3, during installation, lucifuge pipe 5 inserts this pipe connecting 4, the outer wall of lucifuge pipe 7 and the inwall of pipe connecting 4 are that slidable sealing coordinates, namely only have under external force, lucifuge pipe 5 can slide up and down along the inwall of pipe connecting 4, when not having External Force Acting, lucifuge pipe 5 is fixed in pipe connecting 4 actionless, and is processed with 3 flange collars 7 at the outer wall of lucifuge pipe 5, frictional force can be increased, be conducive to the fixing of lucifuge pipe, also can increase stopping property, be conducive to the preservation of the lucifuge material in lucifuge pipe, the upper end closed of lucifuge pipe 5, lower ending opening, during use, opens capping, will see that the labile composition of light adds in lucifuge pipe 5, then lucifuge plug 6 beyond the Great Wall, pushes in pipe connecting, cover capping by lucifuge pipe 5 and lucifuge plug 6.This lucifuge plug 6 also opaque plastic material is made, and under external force, during pressing lucifuge pipe, lucifuge plug can be ejected pipe connecting by lucifuge pipe, sees that the labile material of light just falls into outer tube smoothly in such lucifuge pipe.When not having external force, lucifuge material is sealed in lucifuge pipe by lucifuge plug.
In order to observe the cultivation situation of aerogens, in outer tube 1 be inverted a transparent plastics make in pipe 2, certain interval is left between the outer wall of interior pipe 2 and the inwall of outer tube 1, interior pipe 2 one end is closed, one end open, its opening end down, and a segment distance is left in the bottom of opening end and outer tube 1, in the present embodiment, interior pipe is placed in 1/3 place of outer tube, and the substratum in outer tube did not have the top of interior pipe, be provided with 4 union levers 21 between the outer wall of interior pipe 2 and the inwall of outer tube 1, interior pipe 2 was fixed in outer tube 1 by union lever 21 firmly.Here interior pipe is equivalent to a Du Shi tubule, when testing in prior art, generally Du Shi tubule is inverted in test tube, observe the intratubular gas of Du Shi, and place the operating skill of Du Shi tubule to scientific research personnel and there are certain requirements, if it is improper to place, can makes there is bubble in Du Shi tubule, affect observations.And in the present invention, the mode adopting Du Shi tubule to be fixedly connected with outer tube, eliminates above-mentioned trouble, makes observation more accurate.
When adopting test tube of the present invention to test, we can adopt one-handed performance, and namely left hand takes test tube, and the right hand is taken transfering loop or liquid-transfering gun to add and treated test sample, can greatly reduce pollution.Prior art adopts stopper to fill in the method for test tube, opens stopper inconvenience, and easily pollutes nutrient solution.This test tube adopts one-handed performance, both facilitates, again not vulnerable to pollution; And conveniently opening capping 3, we are radially provided with a flange 31 in the side of capping 3, along the root of flange 31 to downward-extension one press section 32, during operation, thumb pins press section 32, more upwards backs down flange 31, thus raise capping 3, easy to operate, quick.
In order to prevent whole for lucifuge pipe 5 by having arrived in pipe connecting 4, can be arranged with at lucifuge pipe 5 outer that a loop is protruding or several salient point is also passable, be generally to establish in the end of lucifuge pipe a loop protruding, like this, even if overexert, also can by lucifuge pipe gear in capping upper end.In the present embodiment, be enclose a loop projection 8 at the outer of the upper end of lucifuge pipe.In order to prevent being pressed into lucifuge pipe because of carelessness in transport or moving process, the material in lucifuge pipe is caused to drop in outer tube, can at lucifuge pipe outer race collar 9, collar 9 is common collar of the prior art, lucifuge pipe is fixed between annular projection 8 and capping 3 by it, during use, pull up collar, easily.The present invention does not need proofer to prepare the set-up procedure such as substratum and sterilizing, directly can add and treat test sample quick test, convenient, greatly reduces contaminated probability; Lucifuge pipe of the present invention can be deposited in advance and see the labile composition of light, makes the extended shelf-life of substratum; Cost of the present invention is lower, easy and simple to handle, and aerogenesis situation is observed accurately.

Claims (6)

1. an aerogens quick test pipe, comprises the outer tube (1) of lower end closed and is located at the capping (3) at outer tube (1) mouth of pipe place, it is characterized in that: described capping (3) upper surface is to having the pipe connecting (4) passed for a lucifuge pipe (5); The upper end closed of described lucifuge pipe (5), lower ending opening, the outer wall of lucifuge pipe (5) coordinates with the inwall slidable sealing of pipe connecting (4); The lower end of pipe connecting (4) is provided with a lucifuge plug (6), and this lucifuge plug (6) slidably coordinates with pipe connecting (4); Be installed with the interior pipe (2) of one end open in described outer tube (1), down, and gap is left in the bottom of opening end and outer tube (1) to its opening end, leaves gap between the outer wall of interior pipe (2) and the inwall of outer tube (1); The outer wall of described lucifuge pipe (5) is provided with flange collar (7), and lucifuge pipe (5) is coordinated with pipe connecting (4) inner wall sealing by this flange collar (7); The outer of described lucifuge pipe (5) is arranged with an annular projection (8); Described outer tube (1) and interior pipe (2) are transparent material; The outer of described lucifuge pipe (5) is arranged with a collar (9), and this collar (9) is positioned between the upper surface of described annular projection (8) and capping (3).
2. a kind of aerogens quick test pipe according to claim 1, is characterized in that: the side of described capping (3) is radially provided with flange (31), along flange (31) inner side to having press section (32).
3. a kind of aerogens quick test pipe according to claim 2, is characterized in that: described interior pipe (2) is connected with outer tube (1) by arranging union lever (21).
4. a kind of aerogens quick test pipe according to claim 3, is characterized in that: described capping (3) is located at the mouth of pipe place of outer tube by hinged mode.
5. a kind of aerogens quick test pipe according to claim 4, is characterized in that: described outer tube (1), interior pipe (2) are lucite pipe.
6. a kind of aerogens quick test pipe according to claim 1, is characterized in that: described lucifuge pipe, lucifuge plug are plastics tubing.
CN201210155176.5A 2012-05-18 2012-05-18 A kind of aerogens quick test pipe Active CN103421684B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201210155176.5A CN103421684B (en) 2012-05-18 2012-05-18 A kind of aerogens quick test pipe

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201210155176.5A CN103421684B (en) 2012-05-18 2012-05-18 A kind of aerogens quick test pipe

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CN103421684A CN103421684A (en) 2013-12-04
CN103421684B true CN103421684B (en) 2016-04-20

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106520539B (en) * 2016-12-05 2017-11-28 菏泽学院 A kind of microorganism produces sour aerogenesis experiment and sulfur-containing amino acid test experience uses instrument
CN109679835A (en) * 2019-01-31 2019-04-26 济南市疾病预防控制中心 A kind of instant culture apparatus for most probable number method detection flora

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* Cited by examiner, † Cited by third party
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CN2322918Y (en) * 1997-12-04 1999-06-09 蒋钱毛 Bottle cover containing instant particles for preparing beverage
US20040166556A1 (en) * 2003-02-21 2004-08-26 Cullimore Dennis Roy Analytic device for the detection and quantification of fermentative cultured activities
CN2611899Y (en) * 2003-04-07 2004-04-14 河北农业大学 Fast detecting tube for coliform bacteria
CN202576419U (en) * 2012-05-18 2012-12-05 杭州创新生物检控技术有限公司 Aerogens quick-test tube

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