CN201971832U - Sampling device for fermentation tank - Google Patents
Sampling device for fermentation tank Download PDFInfo
- Publication number
- CN201971832U CN201971832U CN 201120045278 CN201120045278U CN201971832U CN 201971832 U CN201971832 U CN 201971832U CN 201120045278 CN201120045278 CN 201120045278 CN 201120045278 U CN201120045278 U CN 201120045278U CN 201971832 U CN201971832 U CN 201971832U
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- diaphragm valve
- sterile air
- fermentor tank
- valve
- steam
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a sampling device for a fermentation tank, which comprises the fermentation tank, a sampling pipe, a sterile air pipe network and a steam pipe network. The sampling pipe penetrates through the upper wall of the fermentation tank, a connecting pipeline of the sterile air pipe network is divided into two channels at a sterile air input port, one channel is connected with an air inlet pipe via a diaphragm valve F2, and the other channel is connected with a diaphragm valve F4 of the steam pipe network via a diaphragm valve F1 and a diaphragm valve F3. A steam connecting pipeline is divided into two channels at a steam input port via a diaphragm valve F4 and a diaphragm valve F5, one channel penetrates through the upper wall of the fermentation tank via a diaphragm valve F6 and is connected with the sampling pipe, and the other channel is communicated with a sampling port via a diaphragm valve F7. The sampling device is convenient in sampling and fine in sealing performance, is safe and reliable, reduces competitor contamination, and simultaneously solves the problem of wastage of fermentation fluid.
Description
Technical field
The utility model relates to a kind of fermentation equipment, and especially a kind of sampling unit that is used to extract fermentation broth sample belongs to technical field of bioengineering.
Background technology
Biological fermentation equipment is the key equipment in the microbial technique exploitation, is most important in the industrial fermentation, most widely used equipment, and as the indispensable device of microorganism culturing, the height of its efficient plays crucial effects to the cultivation of microorganism.In the process of biological fermentation, must often extract fermentation broth sample and detect, whether normally carry out to analyze fermentation reaction, in time carry out fluid infusion.Existing fermentor tank sample position has following two kinds usually: a kind of sample position is called " sampling down " in the fermentor tank bottom; Another kind of sample position is called " going up sampling " on fermentor tank top.
When adopting " sampling down ", because stopple coupon is installed in the fermentor tank bottom, inconvenient operation, stopping property is relatively poor simultaneously, vulnerable to pollution; When adopting " going up sampling ", stopple coupon inserts in it from fermentor tank top, utilizes the gaseous tension that fermentation forms in the fermentor tank that fermented liquid is extruded sampling from stopple coupon, though this kind structure is easy to operate, but the fermentation broth sample vulnerable to pollution that extrudes can not reuse, causes bigger waste.In addition, because stopple coupon inserts the fermented liquid depths from top to bottom, also leave fermented liquid in the stopple coupon of sampling back, this fermented liquid rests in the stopple coupon, can not with fermented liquid thorough mixing in the jar, can not represent the truth of fermented liquid in the fermentor tank again, therefore, all this part fermented liquid will be bled off during sampling.The sampling frequency of most of fermentation is all higher, and the fermented liquid capacity that bleeds off is bigger, has caused bigger waste, has strengthened production cost.
Summary of the invention
The purpose of this utility model provides no fermented liquid stop in the stopple coupon of a kind of sampling back, and assorted bacterium is difficult for polluting, and can not cause the fermentor tank sampling unit of fermented liquid loss.
The utility model is achieved by the following technical programs:
A kind of fermentor tank sampling unit comprises fermentor tank, stopple coupon, sterile air pipe network and steam pipe system that strap clamp overlaps, and described stopple coupon passes fermentor tank top tank skin, and is adjacent with fermentor tank top tank skin; Described sterile air pipe network comprises diaphragm valve F1, diaphragm valve F2, diaphragm valve F3 and sterile air pipe network connecting pipeline, sterile air pipe network connecting pipeline is divided into two-way from the sterile air input aperture, the diaphragm valve F2 of leading up to links to each other with inlet pipe, and another road links to each other with the diaphragm valve F4 of steam pipe system by diaphragm valve F1, diaphragm valve F3; Steam pipe system comprises diaphragm valve F4, diaphragm valve F5, diaphragm valve F6, diaphragm valve F7 and steam pipe system connecting pipeline, the steam connection tube road is divided two-way from vapor inlet port after by diaphragm valve F4, diaphragm valve F5, the diaphragm valve F6 of wherein leading up to passes fermentor tank top tank skin and connects stopple coupon, and thief hole is led to by diaphragm valve F7 in another road.The purpose of this utility model can also further realize by following technical measures.
Aforesaid fermentor tank sampling unit, wherein said diaphragm valve F3, diaphragm valve F6 have vent valve F31, vent valve F61 respectively; Described connecting pipeline is the inside and outside polishing pipe of stainless steel.
The utility model sampling convenience, good airproof performance, safe and reliable, reduced living contaminants, also solved the problem that fermented liquid is wasted simultaneously.
Advantage of the present utility model and characteristics will illustrate by the non-limitative illustration of following preferred embodiment and explain that these embodiment only provide as an example with reference to accompanying drawing.
Description of drawings
Fig. 1 is a structural representation of the present utility model.
Embodiment
The utility model is described in further detail below in conjunction with drawings and Examples.
As shown in Figure 1, the utility model comprises fermentor tank 1, stopple coupon 2, sterile air pipe network 3 and the steam pipe system 4 of strap clamp cover, the fermentor tank 1 employing trade mark of strap clamp cover is that the stainless material of SUS316L is made, motor 51 step-down gears 52 of whipping appts 5 are fixed on fermentor tank 1 top, motor 51 drives stir shaft 53 by step-down gear 52 and rotates, and stirs fermented liquid thereby drive agitating vane 54.Stopple coupon 2 passes fermentor tank 1 top tank skin and turns round vertically downward, near fermentor tank 1 top tank skin.Sterile air pipe network 3 comprises diaphragm valve F1, diaphragm valve F2, diaphragm valve F3 and sterile air pipe network connecting pipeline 31, sterile air pipe network connecting pipeline 31 is divided into two-way from sterile air input aperture 32, the diaphragm valve F2 of leading up to links to each other with inlet pipe 6, and another road links to each other with the diaphragm valve F4 of steam pipe system by diaphragm valve F1, pipeline I 311, diaphragm valve F3, pipeline IX 418, pipeline III 412.Steam pipe system 4 comprises diaphragm valve F4, diaphragm valve F5, diaphragm valve F6, diaphragm valve F7 and steam pipe system connecting pipeline 41, steam connection tube road 41 is divided two-way from vapor inlet port 42 after by pipeline II 411, diaphragm valve F4, pipeline III 412, pipeline IX 418, diaphragm valve F5, wherein leading up to pipeline V 414, pipeline VI 415, diaphragm valve F6 passes fermentor tank 1 top tank skin and connects stopple coupon 2, and thief hole 42 is led to by pipeline VII 416, diaphragm valve F7, pipeline VIII 417 in another road.
Diaphragm valve F1～diaphragm valve F7 is the diaphragm valve of stainless steel, high temperature resistant silicon glue film, and diaphragm valve F3, diaphragm valve F6 have vent valve F31, vent valve F61 respectively; Sterile air pipe network connecting pipeline 3, steam pipe system connecting pipeline 4 are the inside and outside polishing pipe of stainless steel.Be difficult for getting rusty, avoid the living contaminants fermented liquid.
Use sampling process of the present utility model as follows:
1) opens diaphragm valve F4, diaphragm valve F5 and the diaphragm valve F7 of steam pipe system 4 earlier, allow steam that pipeline II 411, pipeline III 412, pipeline IV 413, pipeline V 414, pipeline VII 416, the pipeline VIII 417 on steam connection tube road 41 are carried out disinfection; Open the vent valve F31 of diaphragm valve F3, the vent valve F61 of diaphragm valve F6 simultaneously, allow steam that pipeline VI 415, pipeline IV 413 are carried out disinfection.
2) after sterilization finishes, close the vent valve F31 of diaphragm valve F4, diaphragm valve F5 and diaphragm valve F7 and diaphragm valve F3, the vent valve F61 of diaphragm valve F6, sample bottle is connected on thief hole 42 times.
3) open diaphragm valve F1, diaphragm valve F3, diaphragm valve F5 and diaphragm valve F6, sterile air from sterile air inlet 32 by the road I 311, pipeline IX 418, pipeline III 412, pipeline V 414, pipeline VI 415 through diaphragm valve F6 to the stopple coupon 2 that feeds in the fermentor tank 1, fermented liquid original in it is headed into fermentor tank 1, make liquid emptyings in the stopple coupon 2.
4) close diaphragm valve F1, diaphragm valve F3, diaphragm valve F5 and diaphragm valve F6.Open diaphragm valve F6 and diaphragm valve F7, owing in the fermentor tank 1 gaseous tension is arranged, fermented liquid is by stopple coupon 2, through in diaphragm valve F6, pipeline VI 415, pipeline VII 416, diaphragm valve F7 and the pipeline VIII 417 back person who lives in exile's sampling jugs.
5) after sampling finishes, close diaphragm valve F6 and diaphragm valve F7, remove the sample thief bottle.
6) open diaphragm valve F4, diaphragm valve F5 and diaphragm valve F7 once more, allow steam to pipeline II 411, pipeline III 412, pipeline IV 413,, pipeline V 414, pipeline VII 416 and pipeline VIII 417 carry out disinfection; Open the vent valve F31 of diaphragm valve F3, the vent valve F61 of diaphragm valve F6 simultaneously, allow steam that pipeline IX 418 and pipeline VI 415 are carried out disinfection, after sterilization finishes, close the vent valve F31 of diaphragm valve F4, diaphragm valve F5 and diaphragm valve F7 and film valve F3, the vent valve F61 of diaphragm valve F6, whole sampling process finishes.
In addition to the implementation, the utility model can also have other embodiments, and all employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop in the protection domain of the utility model requirement.
Claims (3)
1. a fermentor tank sampling unit comprises fermentor tank, stopple coupon that strap clamp overlaps, and described stopple coupon passes fermentor tank top tank skin, and is adjacent with fermentor tank top tank skin; It is characterized in that: also comprise sterile air pipe network, steam pipe system, described sterile air pipe network comprises diaphragm valve F1, diaphragm valve F2, diaphragm valve F3 and sterile air pipe network connecting pipeline, sterile air pipe network connecting pipeline is divided into two-way from the sterile air input aperture, the diaphragm valve F2 of leading up to links to each other with inlet pipe, and another road links to each other with the diaphragm valve F4 of steam pipe system by diaphragm valve F1, diaphragm valve F3; Steam pipe system comprises diaphragm valve F4, diaphragm valve F5, diaphragm valve F6, diaphragm valve F7 and steam pipe system connecting pipeline, the steam connection tube road is divided two-way from vapor inlet port after by diaphragm valve F4, diaphragm valve F5, the diaphragm valve F6 of wherein leading up to passes fermentor tank top tank skin and connects stopple coupon, and thief hole is led to by diaphragm valve F7 in another road.
2. fermentor tank sampling unit as claimed in claim 1 is characterized in that: described diaphragm valve F3, diaphragm valve F6 have vent valve F31, vent valve F61 respectively.
3. fermentor tank sampling unit as claimed in claim 1 is characterized in that: described sterile air pipe network connecting pipeline, steam pipe system connecting pipeline are the inside and outside polishing pipe of stainless steel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120045278 CN201971832U (en) | 2011-02-23 | 2011-02-23 | Sampling device for fermentation tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120045278 CN201971832U (en) | 2011-02-23 | 2011-02-23 | Sampling device for fermentation tank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201971832U true CN201971832U (en) | 2011-09-14 |
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ID=44576939
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120045278 Expired - Fee Related CN201971832U (en) | 2011-02-23 | 2011-02-23 | Sampling device for fermentation tank |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201971832U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107063767A (en) * | 2017-06-14 | 2017-08-18 | 山东省科学院生物研究所 | A kind of sampler and fermentation tank sterile sampling method |
| CN108034569A (en) * | 2018-02-06 | 2018-05-15 | 重庆医科大学附属永川医院 | A kind of clinical laboratory's bacteriological incubator |
-
2011
- 2011-02-23 CN CN 201120045278 patent/CN201971832U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107063767A (en) * | 2017-06-14 | 2017-08-18 | 山东省科学院生物研究所 | A kind of sampler and fermentation tank sterile sampling method |
| CN107063767B (en) * | 2017-06-14 | 2019-05-21 | 山东省科学院生物研究所 | A kind of sampler and fermentor sterile sampling method |
| CN108034569A (en) * | 2018-02-06 | 2018-05-15 | 重庆医科大学附属永川医院 | A kind of clinical laboratory's bacteriological incubator |
| CN108034569B (en) * | 2018-02-06 | 2021-07-27 | 重庆医科大学附属永川医院 | Clinical laboratory's bacterial incubator |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110914 Termination date: 20130223 |