CN215767785U - A sampling tube for microorganism detects - Google Patents
A sampling tube for microorganism detects Download PDFInfo
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- CN215767785U CN215767785U CN202121958674.4U CN202121958674U CN215767785U CN 215767785 U CN215767785 U CN 215767785U CN 202121958674 U CN202121958674 U CN 202121958674U CN 215767785 U CN215767785 U CN 215767785U
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Abstract
The utility model discloses a sampling tube for microbial detection, which comprises: the inner tube is used for containing sampling liquid; an outer tube sleeved outside the inner tube to protect the inner tube; a pipe cap for sealing the inner pipe and the outer pipe, an inner peripheral portion of the pipe cap being provided with an internal thread for screw-coupling with the inner pipe; the outer periphery of the pipe cover is provided with an external thread used for being in threaded connection with the outer pipe. In the transfer process that this sampling tube that will be equipped with the sampling liquid transmits to the quality detection zone from cell production district, because the outer tube has outside the inner tube, consequently can avoid the outer wall of inner tube to be infected with the microorganism in the environment, before the detection, can spray 75% alcohol in order to kill the environmental microorganism in the outer tube department of sampling tube earlier, then take off the outer tube, can directly put into biological safety cabinet with the inner tube, need not to spray 75% alcohol to the inner tube and kill the external environment microorganism, consequently, can avoid alcohol fuzzy or wash out the information of previous mark.
Description
Technical Field
The utility model relates to the technical field of medical instruments, in particular to a sampling tube for microbial detection.
Background
In biomedical research, cell culture is usually involved, and in order to detect whether cells are polluted, a small amount of culture supernatant is taken at different time nodes in the cell culture process, and detection of microorganisms (such as aerobic bacteria, anaerobic bacteria, mycoplasma, endotoxin and the like) is carried out.
When taking a sample to the culture supernatant, adopt different volume's centrifuging tube or EP pipe to take a sample at present, then utilize marker pen at the outer wall mark sample serial number of centrifuging tube or EP pipe and the censorship project of examining to use the sealing film in mouth of pipe and tube cap position to seal, in order to prevent external environment pollution, then, will be equipped with the centrifuging tube or EP pipe of sample and transmit to the quality detection area from the cell production district, take out the sample supernatant from centrifuging tube or EP pipe by the quality control personnel and carry out corresponding detection.
However, in the transferring process of the centrifugal tube or the EP tube containing the sample, the passing area is not all sterile environment, so the outer wall of the centrifugal tube or the EP tube is easily contaminated by the microbes in the environment and brings the microbes into the detection chamber, and the detection personnel inevitably contact the outer wall of the centrifugal tube or the EP tube during the detection, so that in the operation process of detecting the sample supernatant of the centrifugal tube or the EP tube, the hidden danger that the microbes on the outer wall of the centrifugal tube or the EP tube fall into the detection tube exists, and the false positive of the sample is easily caused.
In order to avoid the situation that the environmental microorganisms on the outer wall of the centrifuge tube or the EP tube drop into the detection tube carelessly, before the centrifuge tube or the EP tube filled with the sample is sent into the detection chamber and is put into the biological safety cabinet, 75% alcohol is needed to be adopted to spray and sterilize the outer wall of the centrifuge tube or the EP tube so as to kill the microorganisms possibly infected with the outer wall of the centrifuge tube or the EP tube. However, because the outer wall of centrifuging tube or EP pipe is marked with sample number and censorship project, when the outer wall of centrifuging tube or EP pipe sprays 75% alcohol, it is obscure very easily to lead to the sample number and the censorship project of the outer wall mark of centrifuging tube or EP pipe, when once only detecting a plurality of samples, will not distinguish the number that different centrifuging tubes or EP pipes correspond, leads to censorship sample and testing result to mismatch, influences cell production and use. Moreover, the ink dissolved in the alcohol may contaminate the sample or the detection reagent, thereby affecting the detection result.
In summary, it is an urgent need to solve the problem of the present technical skill to kill the environmental microorganisms on the outer wall of the sampling tube and avoid the ambiguity of the labeled information on the outer wall of the sampling tube.
SUMMERY OF THE UTILITY MODEL
Accordingly, the present invention is directed to a sampling tube for detecting microorganisms, which can prevent the outer wall of the sampling tube from being obscured by the label information due to the sterilization of the outer wall of the sampling tube.
In order to achieve the above purpose, the utility model provides the following technical scheme:
a sampling tube for the detection of microorganisms comprising:
the inner tube is used for containing sampling liquid;
the outer pipe is sleeved outside the inner pipe to protect the inner pipe;
a tube cap for sealing the inner tube and the outer tube, an inner peripheral portion of the tube cap being provided with an internal thread for screw-coupling with the inner tube; and the outer periphery of the pipe cover is provided with an external thread which is used for being in threaded connection with the outer pipe.
Preferably, the internal thread is opposite in thread direction to the external thread.
Preferably, the top end of the tube cover is provided with a convex part which protrudes out of the surface of the tube cover so as to be convenient to take.
Preferably, the outer side of the convex part is provided with a plurality of convex ribs protruding out of the surface of the convex part.
Preferably, the inner tube and the outer tube are both transparent tubes.
Preferably, the outer side of the inner tube is provided with scale marks.
Preferably, the outer side of the inner tube is provided with a frosted area so that the marking pen can write marking information conveniently.
Preferably, the bottom of the inner tube is of an inverted cone structure.
Preferably, a plastic package is included for enclosing the coupon.
Preferably, the difference between the inner diameter of the outer tube and the outer diameter of the inner tube is 5 to 10 mm.
According to the sampling tube for detecting microorganisms, the inner tube is nested in the outer tube, so that the inner tube and the outer tube form the double-layer nested sampling tube, the inner tube and the outer tube are sealed by the tube cover, the inner tube and the outer tube are connected, and the outer tube can be used for protecting the inner tube. Like this, in the transfer process of transferring this sampling tube that will be equipped with the sampling liquid from cell production district to quality detection district, because the outer tube has outward of inner tube, consequently can avoid the outer wall of inner tube to be infected with the microorganism in the environment, before the detection, can spray 75% alcohol in order to kill off environmental microorganism in the outer tube department of sampling tube earlier, then take off the outer tube, can directly put into biological safety cabinet with the inner tube, need not to spray 75% alcohol to the inner tube and kill off external environment microorganism, consequently, can avoid the alcohol to obscure or wash away the information of previous mark.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a schematic diagram of a sampling tube for detecting microorganisms according to an embodiment of the present invention;
FIG. 2 is a schematic structural view of the cap of FIG. 1;
fig. 3 is a schematic view of the structure of fig. 2 from another perspective (wherein the external threads of the tube cap are not shown).
The reference numerals in fig. 1 to 3 are as follows:
1 is an inner tube, 11 is a scale mark, 12 is a frosted area, 13 is an inverted cone structure, 2 is an outer tube, 3 is a tube cover, 31 is an internal thread, 32 is an external thread, 33 is a convex part, and 331 is a convex edge.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The core of the utility model is to provide a sampling tube for microorganism detection, which can avoid the fuzzy marking information of the outer wall of the sampling tube caused by killing the outer wall of the sampling tube.
Referring to fig. 1 to 3, fig. 1 is a schematic structural view of a sampling tube for detecting microorganisms according to an embodiment of the present invention; FIG. 2 is a schematic structural view of the cap of FIG. 1; fig. 3 is a schematic view of the structure of fig. 2 from another perspective (wherein the external threads of the tube cap are not shown).
The utility model provides a sampling tube for microbial detection, which comprises an inner tube 1, an outer tube 2 and a tube cap 3, wherein the inner tube 1 is used for containing sampling liquid, the outer tube 2 is sleeved outside the inner tube 1 to protect the inner tube 1, the tube cap 3 is used for sealing the inner tube 1 and the outer tube 2, and the inner peripheral part of the tube cap 3 is provided with an internal thread 31 which is in threaded connection with the inner tube 1; the outer periphery of the cap 3 is provided with a male screw 32 for screwing with the outer tube 2.
That is, the inner tube 1 is nested in the outer tube 2, so that the inner tube 1 and the outer tube 2 form a double-layer nested sampling tube, the inner tube 1 and the outer tube 2 are sealed by the tube cap 3, the inner tube 1 and the outer tube 2 are connected, and the outer tube 2 can be used for protecting the inner tube 1.
When the sampling tube is used for sampling, sample liquid is filled into the inner tube 1, the tube cap 3 is screwed with the inner tube 1, then information such as sample numbers and inspection items is marked on the outer side of the inner tube 1 by using a marking pen, then the outer tube 2 is sleeved outside the inner tube 1, and the outer tube 2 and the tube cap 3 are screwed, so that sampling can be completed. Or, the information may be marked on the outer side of the inner tube 1, the sample solution is filled into the inner tube 1, the outer tube 2 is sleeved outside the inner tube 1, and finally the tube cap 3 is screwed with the inner tube 1 and the outer tube 2 at the same time to complete sampling.
After the completion sampling, in the transfer process that this sampling tube that will be equipped with the sampling liquid transmits to the quality detection area from cell production district, because inner tube 1 has outer tube 2 outward, consequently can avoid the outer wall of inner tube 1 to be infected with the microorganism in the environment, before the detection, can spray 75% alcohol in order to kill the environment microorganism in outer tube 2 department of sampling tube earlier, then take off outer tube 2, can directly put into biological safety cabinet with inner tube 1, need not to spray 75% alcohol to inner tube 1 and kill external environment microorganism, consequently, can avoid alcohol fuzzy or wash out the information of previous mark.
Further, in the above embodiment, the female screw 31 is opposite to the male screw 32 in the screwing direction in order to prevent the inner tube 1 from being loosened and removed when the outer tube 2 is unscrewed from the cap 3. Correspondingly, the threads on the inner tube 1 and the outer tube 2 for screwing with the tube cap 3 are in opposite directions. For example, when the thread on the inner tube 1 is rotated counterclockwise, the thread on the outer tube 2 is rotated clockwise, or vice versa, so that when the outer tube 2 and the cap 3 are unscrewed, there is a tendency to make the fit between the cap 3 and the inner tube 1 tighter, so that the inner tube 1 can be prevented from being loosened or falling off from the cap 3.
In addition, in order to facilitate the taking of the tube cap 3 and the assembly of the tube cap 3 with the inner tube 1 and the outer tube 2, on the basis of the above embodiment, the top end of the tube cap 3 is provided with a protrusion 33 protruding from the surface of the tube cap 3. That is, when the cap 3 is assembled with the inner tube 1 and the outer tube 2, respectively, the cap 3 can be assembled with the inner tube 1 and the outer tube 2, respectively, by taking the projection 33, by applying a hand force to the projection 33.
Preferably, the boss 33 is a columnar boss 33, for example, the boss 33 is a cylindrical boss 33.
Further, in order to increase the friction force between the hand and the protrusion 33, on the basis of the above embodiment, the outer side of the protrusion 33 is provided with a plurality of ribs 331 protruding from the surface thereof, that is, the embodiment increases the friction force when the user takes the protrusion 33 by hand by providing the ribs 331 on the outer side of the protrusion 33, so as to apply the hand force to the protrusion 33 to screw the tube cover 3 with the inner tube 1 and the outer tube 2, respectively.
Preferably, as shown in fig. 1, the rib 331 is a vertical rib 331.
In addition, in order to facilitate observation of the marking information on the inner tube 1 and to facilitate observation of the sampling liquid in the inner tube 1, the inner tube 1 and the outer tube 2 are transparent tubes in addition to the above-described embodiment. That is, the inner tube 1 and the outer tube 2 both have perspective so that the marking information outside the inner tube 1 can be observed at any time from outside the outer tube 2, and simultaneously, the condition of the sampling liquid in the inner tube 1 can be observed favorably.
Further, in order to facilitate observation of the amount of the sample in the inner tube 1, in addition to the above-described embodiment, the outer side of the inner tube 1 is provided with scale lines 11. That is, the present embodiment indicates the volume of the sample liquid in the inner tube 1 by using the scale 11, so that different volumes of the sample liquid remain in the inner tube 1 according to different detection items. Preferably, the graduation mark 11 comprises a graduation mark 11 of 5ml, a graduation mark 11 of 10ml and a graduation mark 11 of 20ml, so as to adapt to the requirements of different detection items.
In addition to the above embodiments, a frosted area 12 is provided on the outer side of the inner tube 1 in order to mark information on the outer side of the inner tube 1. That is, in this embodiment, the frosted area 12 can be marked with information such as a sample number and a delivery item, and the frosted area 12 is convenient for a marker to write and is beneficial for making the marked information clearly exist outside the inner tube 1.
Preferably, the frosted area 12 and the graduation marks 11 are arranged symmetrically with respect to the axis of the inner tube 1.
In addition, in order to facilitate the assembly of the inner tube 1 and the outer tube 2, in addition to the above embodiment, the bottom of the inner tube 1 has an inverted cone structure 13. It can be understood that, when the inner tube 1 is nested in the outer tube 2, the bottom of the inner tube 1 firstly enters the outer tube 2 from the orifice of the outer tube 2, and the bottom of the inner tube 1 is designed to be the inverted cone structure 13, which is beneficial to the bottom of the inner tube 1 to be nested in the outer tube 2 more smoothly.
It should be noted that, in the above embodiments, the specific structure of the bottom of the outer tube 2 is not limited, and preferably, the bottom of the outer tube 2 is a planar structure, and of course, the bottom of the outer tube 2 may also be a convex bottom structure protruding outwards.
Further, in the above embodiments, in order to facilitate the preservation of the sampling tube for microorganism detection, a plastic package for accommodating the sampling tube is further included on the basis of the above embodiments.
That is, the sampling tube for microorganism detection is sealed in a plastic package before sampling, so that the sampling tube is isolated from the outside by the plastic package.
Preferably, the plastic package may be sterilized with ethylene oxide to ensure sterility of the internal environment.
Before cell culture sampling, spraying 75% alcohol on the plastic package of the sampling tube for microorganism detection to sterilize, then placing the plastic package containing the sampling tube for microorganism detection in a transfer window leading to a cell culture chamber, and at the moment, further optionally carrying out ultraviolet irradiation on the plastic package to further sterilize; then, the laboratory operator opens the plastic package in the cultivation room and puts the sampling tube for microorganism detection into a biosafety cabinet to be used for obtaining a sample liquid.
It should be noted that, in each of the above embodiments, the inner tube 1 and the outer tube 2 are preferably sterilized by ethylene oxide sterilization.
Further, in each of the above embodiments, specific dimensions of the inner tube 1 and the outer tube 2 are not limited, and preferably, a difference between an inner diameter of the outer tube 2 and an outer diameter of the inner tube 1 is 5 to 10 mm based on the above embodiments.
The embodiments are described in a progressive manner in the specification, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The sampling tube for detecting microorganisms provided by the present invention is described in detail above. The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its core concepts. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.
Claims (10)
1. A coupon for the detection of microorganisms, comprising:
an inner tube (1) for containing a sample liquid;
an outer tube (2) which is sleeved outside the inner tube (1) to protect the inner tube (1);
a tube cap (3) for sealing the inner tube (1) and the outer tube (2), an inner peripheral portion of the tube cap (3) being provided with an internal thread (31) for screw-connecting with the inner tube (1); and the outer periphery of the pipe cover (3) is provided with an external thread (32) for being in threaded connection with the outer pipe (2).
2. Sampling tube for the detection of microorganisms according to claim 1, characterized in that the internal thread (31) is opposite to the screwing direction of the external thread (32).
3. A sampling tube for the detection of microorganisms as claimed in claim 1, characterized in that the top end of the tube cover (3) is provided with a protrusion (33) protruding from the surface of the tube cover (3) for easy access.
4. A sampling tube for the detection of microorganisms as claimed in claim 3, characterized in that the outer side of the boss (33) is provided with a plurality of ribs (331) protruding from the surface thereof.
5. A sampling tube for the detection of microorganisms according to any one of claims 1 to 4, characterized in that both the inner tube (1) and the outer tube (2) are transparent tubes.
6. A sampling tube for the detection of microorganisms as claimed in claim 5, characterized in that the inner tube (1) is provided with graduation marks (11) on its outside.
7. A sampling tube for the detection of microorganisms according to any one of claims 1 to 4, characterized in that the outer side of the inner tube (1) is provided with a frosted area (12) to facilitate the writing of marking information by a marker.
8. A sampling tube for the detection of microorganisms according to any one of claims 1 to 4, characterized in that the bottom of the inner tube (1) is of an inverted cone-shaped structure (13).
9. A sampling tube for the detection of microorganisms as claimed in any one of claims 1 to 4, further comprising a plastic package for enclosing the sampling tube.
10. A sampling tube for the detection of microorganisms according to any of claims 1 to 4, characterized in that the difference between the inner diameter of the outer tube (2) and the outer diameter of the inner tube (1) is 5 to 10 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121958674.4U CN215767785U (en) | 2021-08-19 | 2021-08-19 | A sampling tube for microorganism detects |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121958674.4U CN215767785U (en) | 2021-08-19 | 2021-08-19 | A sampling tube for microorganism detects |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215767785U true CN215767785U (en) | 2022-02-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121958674.4U Active CN215767785U (en) | 2021-08-19 | 2021-08-19 | A sampling tube for microorganism detects |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215767785U (en) |
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2021
- 2021-08-19 CN CN202121958674.4U patent/CN215767785U/en active Active
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Address after: 266000 No. 499, qichangcheng Road, Huangdao District, Qingdao, Shandong Patentee after: Qingdao Ruiyuan Cell Biotechnology Development Co.,Ltd. Address before: 266000 No. 499, qichangcheng Road, Huangdao District, Qingdao, Shandong Patentee before: Qingdao Zhongkang yuanneng Cell Biotechnology Co.,Ltd. |