CN204661699U - A kind of quantitative graduated tube pipetting solution for trace - Google Patents
A kind of quantitative graduated tube pipetting solution for trace Download PDFInfo
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- CN204661699U CN204661699U CN201520322885.7U CN201520322885U CN204661699U CN 204661699 U CN204661699 U CN 204661699U CN 201520322885 U CN201520322885 U CN 201520322885U CN 204661699 U CN204661699 U CN 204661699U
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- suction pipe
- deck
- overcoat
- suction
- suction nozzle
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Abstract
The utility model discloses a kind of quantitative graduated tube pipetting solution for trace, comprise suction pipe, overcoat suction nozzle and connect deck, suction pipe is capillary pipet, Connection Card cover for seat is located at the top of suction pipe and is fixed with one with suction pipe, overcoat suction nozzle is set in and connects on deck, connection deck is provided with multi-turn bulge loop with the combination section of overcoat suction nozzle, in overcoat suction nozzle, is provided with suction blister cavities, inhale blister cavities and be communicated with suction pipe.Suction pipe be connected deck and make by transparent polymer material, the outer tube wall of suction pipe is provided with scale marks.Suction pipe outer tube diameter is 1-1.2mm, and thickness of pipe is 0.2mm.The lower port of suction pipe is oblique sharp opening or flat mouth, and the utility model cost is lower, and good seal performance is non-friable, quantitatively accurately, can ensure that micro-bacterium liquid draws precision; Be convenient to stretch into the health that caliber is less, liquid level is low and in vitro pipette micro solution, can be used in solid medium puncture and use; The routine work that microbiological test is a large amount of, complicated can be adapted to.
Description
Technical field
The utility model relates to experiment equipment field, is specifically related to a kind of quantitative graduated tube pipetting solution for trace.
Background technology
Bacterium micro biochemical assessor is the sterilising medium being specifically designed to bacterial biochemical assay, need by the microbionation that is separated in assessor when carrying out bacterial biochemical assay, then be put in suitable culture environment and cultivate, the Different Results that its carbohydrate metabolism, protein and amino acid metabolism, organic acid salt and ammonium salt utilize by observing, various enzyme produces after utilizing, thus reach the object of being classified, identifying.Bacterium micro biochemical assessor is easy to use because of it, low price, result is comparatively reliable, thus use wider in the Bacterial characterization of veterinary microorganism, for the ease of storing, carrying, transport, and reduce costs, substratum is generally sub-divided in thin glass tube and sells, at present owing to providing manufacturer different, the Glass tubing inside diameter ranges of all kinds of substratum of packing is between 2 ~ 4 mm.
In the aseptic technique process of bacterial biochemical assay, general use liquid-transfering gun and sterilizing rifle head, pipetted from health test tube by micro-bacterium liquid and join biochemical identification pipe, some biochemical identification project also needs separately to add reagent, can judged result after mixing.Because the caliber of bacterium micro biochemical assessor is very little, existing liquid-transfering gun suction nozzle cannot stretch into assessor depths and add bacterium liquid, in addition there is the cause of atmospheric drag in assessor, often bubble is formed between the bacterium liquid added and the interior substratum of pipe, bacterium liquid is made to float over mouth of pipe place, the normal discharger of bacterium liquid pollutes worktable and operator outward, simultaneously, biochemical identification pipe cannot mix reagent by jolting mode, bacterium liquid cannot be mixed with substratum in pipe, often cause the consequence reflected that the result of these certified varieties can not be correct.Also have some certified varieties to need to use solid medium and puncture mode to carry out inoculated bacteria, Ordinary vaccinations pin is flexible, operation inconvenience, and this bacterioid micro biochemical assessor is restricted in use.Secondly the health test tube that in biochemical identification test, the preparation of 2mL bacteria suspension often adopts bore less, because pipe shaft is longer, liquid level is too low, little suction nozzle is not easy to touch liquid level, and the liquid-transfering gun body of a gun stretches into and in vitro easily pollutes, move liquid more difficult time-consuming, it is very inconvenient to operate.
At present, micropipet has become the whether accurate key of assay, and in clinical treatment, mostly to be glass made for micropipet, and it exists following shortcoming:
1, cost is higher, and the suction blister cavities stopping property of suction pipe and its afterbody is poor, and impact moves liquid work;
2, frangible easy to break, easily cause infection, form new source of pollution;
3, quantitatively inaccurate, because the trace of bacteria bacterium liquid measure pipetted is little at every turn, cannot ensure that micro-bacterium liquid draws precision;
4, even, slowly-releasing bacterium liquid in the less bacterium micro biochemical assessor of caliber cannot be stretched into;
5, can not be applicable to carry out bacterium percutaneous puncture-inoculation in solid medium;
6, Laboratory Clean, sterilization, bake process are wasted time and energy, and the sterilization preparation work before using is comparatively loaded down with trivial details, can not adapt to the routine work that microbiological test is a large amount of, complicated.
Utility model content
In order to solve the problems of the technologies described above, the utility model provides a kind of quantitative graduated tube pipetting solution for trace, this quantitative graduated tube good airproof performance, non-friable, can be used for the bacterium liquid inoculation of bacterium micro biochemical assessor, and the pipetting of a small amount of bacterium liquid of lower liquid level in elongated test tube, have easy to use, the feature that security is high.
The purpose of this utility model carrys out specific implementation by the following technical programs:
A kind of quantitative graduated tube pipetting solution for trace, comprise suction pipe and overcoat suction nozzle, also comprise connection deck, described suction pipe is capillary pipet, described Connection Card cover for seat is located at the top of suction pipe and is fixed with one with suction pipe, and described overcoat suction nozzle is set in and connects on deck, and connection deck is provided with multi-turn for strengthening the bulge loop of sealing effectiveness with the combination section of overcoat suction nozzle, in overcoat suction nozzle, be provided with suction blister cavities, described suction blister cavities is communicated with suction pipe.
Further, described suction pipe be connected deck and make by transparent polymer material.
Further, the outer tube wall of described suction pipe is provided with scale marks.
Further, the minimum division value of described scale marks is 5 μ L.
Further, described suction pipe outer tube diameter is 1-1.2mm, and thickness of pipe is 0.2mm.
Further, the lower port of described suction pipe is oblique sharp opening.
Further, the lower port of described suction pipe is flat mouth, and the tube wall of suction pipe is provided with two side openings near lower port place.
Further, described overcoat suction nozzle is made up of silica gel, and overall in warhead form, the center of overcoat suction nozzle is inverted U-shaped open cavity, and described suction blister cavities is set in connect on deck by the U-shaped open cavity of overcoat suction nozzle to be formed with bulge loop close fit.
Further, described connection deck is hollow circuit cylinder body structure, suction pipe be plugged on connect the center of deck and sidewall be connected deck and be compressed to integrally, the end face of suction pipe is concordant with the upper surface being connected deck.
Meanwhile, quantitative graduated tube entirety adopts single Zhi Duli sterilising packaging, the sterilizing of high temperature height or ethane via epoxyethane sterilizing, nontoxic aseptic without thermal source, adopts single bubble-cap plastic packaging, is single use test consumable, can adapts to the routine work that microbiological test is a large amount of, complicated.
A kind of quantitative graduated tube pipetting solution for trace of the utility model, has following beneficial effect:
1, suction pipe is capillary pipet, cost is lower, is provided with the connection deck be fixed with one with suction pipe between suction pipe and overcoat suction nozzle, on connection deck, the combination section of overcoat suction nozzle is provided with multi-turn bulge loop simultaneously, enhance sealing property, ensure to move normally carrying out of liquid work;
2, suction pipe be connected deck and make by transparent polymer material, non-friable;
3, scale marks is set on suction pipe, quantitatively accurately, can ensures that micro-bacterium liquid is drawn and precision high-volume;
4, suction pipe outer tube diameter is set to 1-1.2mm, and thickness of pipe is 0.2mm, is convenient to stretch into the health that caliber is less, liquid level is low and in vitro pipettes trace of bacteria solution, and stretches in bacterium micro biochemical assessor and carry out bacterium liquid application of sample;
5, the lower port of suction pipe is oblique sharp opening or flat mouth, and oblique sharp opening can be used in solid medium puncture and uses; Flat mouth design and lateral opening hole can make the more even of the interior dispersion of bacterium liquid liquid medium within, avoid bubble formation;
6, primary sterilization independent packaging can improve the working efficiency of bacterial biochemical assay experiment greatly, can adapt to the routine work that microbiological test is a large amount of, complicated.
Accompanying drawing explanation
According to drawings and embodiments the utility model is described in further detail below.
Fig. 1 is a kind of structural representation pipetting the quantitative graduated tube of solution for trace of the utility model;
Fig. 2 is a kind of structural representation pipetting quantitative another embodiment of graduated tube of solution for trace of the utility model.
In figure: 1-suction pipe; 2-overcoat suction nozzle; 3-connects deck; 4-bulge loop; 5-inhales blister cavities; 6-scale marks; 7-lower port; 8-side opening.
Embodiment
As shown in Figure 1-2, a kind of quantitative graduated tube pipetting solution for trace described in the utility model embodiment, comprise suction pipe 1 and overcoat suction nozzle 2, also comprise and connect deck 3, suction pipe 1 is capillary pipet, connect deck 3 be set in the top of suction pipe 1 and be fixed with one with suction pipe 1, overcoat suction nozzle 2 is set in and connects on deck 3, connection deck 3 is provided with multi-turn for strengthening the bulge loop 4 of sealing effectiveness with the combination section of overcoat suction nozzle 2, be provided with in overcoat suction nozzle 2 and inhale blister cavities 5, suction blister cavities 5 and suction pipe 1 are socketed by bulge loop 4 and are communicated with, enhance sealing property, control the absorption precision of micro solution thus, improve test accuracy, ensure to move normally carrying out of liquid work.
Preferably, suction pipe 1 be connected deck 3 and make by transparent polymer material, concrete can adopt bright polypropylene (PP) rigid plastics material, high temperature high voltage resistant, organic solvent-resistant and acid and alkali corrosion, tube wall is thinner, inner wall smooth is penetrating, there is certain degree of hardness not yielding, transparent, vertical, unobstructed, and non-friable.
More preferred, the outer tube wall of suction pipe 1 is provided with scale marks 6, quantitatively accurately, can ensures that micro-bacterium liquid draws precision; The minimum division value of scale marks is 5 μ L simultaneously, scale marks is initial by the side near suction pipe lower port, each scale marks is labeled as 5 μ L, 10 μ L, 15 μ L, 20 μ L, 25 μ L successively, quantitatively can pipette trace of bacteria bacterium liquid and carry out application of sample, drawing bacterium liquid capacity is 5-25 μ L scope, is certainly not limited to above-mentioned scope.
Suction pipe 1 outer tube diameter that the trace comprising above-mentioned embodiment pipettes the quantitative graduated tube of solution is the arbitrary value between 1-1.2mm, comprise 1mm and 1.2mm two end values, thickness of pipe is 0.2mm, length is 110-120mm, be conducive to it to stretch into the health that caliber is less, liquid level is low and in vitro pipette micro solution, and facilitate bottom its trace of bacteria biochemical identification pipe inserting caliber 2-4mm and carry out the operation of bacterium application of sample.
Preferably, suction pipe 1 lower port is provided with two kinds of opening pattern: as shown in Figure 1, and the lower port 7 of suction pipe 1 is oblique sharp opening, is conducive to it and carries out bacterium percutaneous puncture-inoculation in solid medium.As shown in Figure 2, the lower port of suction pipe 1 is flat mouth, and the tube wall of suction pipe 1 is provided with two side openings 8 near lower port 7 place, and what contribute to disperseing in the bacterium liquid liquid medium within suction pipe 1 is more even, avoids bubble formation.
Simultaneously, overcoat suction nozzle 2 is made up of silica gel, highly be set to 20mm, width 9mm, overall in warhead form, the center of overcoat suction nozzle 2 is inverted U-shaped open cavity, U-shaped open cavity height is 16mm, accent diameter is 5mm, suction nozzle wall thickness is at about 2mm, good springiness, inhaling blister cavities 5 is be set in connect on deck 3 by the U-shaped open cavity of overcoat suction nozzle 2 to be formed with bulge loop 4 close fit, connecting deck 3 is hollow circuit cylinder body structure, the height connecting deck 3 is 20mm, external diameter is 5mm, thickness is 2mm, multiple bulge loop 4 is uniform at 10mm place, deck upper end, suction pipe 1 be plugged on connect the center of deck 3 and sidewall be connected deck 3 and be compressed to integrally, the end face of suction pipe 1 is concordant with the upper surface being connected deck 3, certainly, suction pipe 1, the size of overcoat suction nozzle 2 and connection deck 3 is not limited to above-mentioned numerical value, can adjust according to embody rule environment.
The utility model is single Zhi Duli sterilising packaging, and the sterilizing of high temperature height or ethane via epoxyethane sterilizing are nontoxic aseptic without thermal source, adopt single bubble-cap plastic packaging, are single use test consumable, can adapt to the routine work that microbiological test is a large amount of, complicated.
The utility model is a kind of pipettes the quantitative graduated tube of solution in use for trace, mainly carries out according to following steps:
1. Preparatory work of experiment: take out bacterial biochemical assay pipe from packing box, with little emery wheel standardized circle bottleneck above tube wall, then firmly fracture assessor, inserts in test-tube stand and (if assessor microbiological contamination or variable color, then can not use, again take one).
2. collecting cells: get the health test tube containing 2ml sterilized water in, with inoculating needle from picking flat board purification of individual bacterium colony in sterilized water, carefully the homogeneous bacterial suspension of 0.5 Maxwell turbidity is made in grinding.
3. inoculate:
3.1 contents are liquid nutrient medium:
Use the quantitative graduated tube of flat mouth opening, often 5-10 μ L bacterium liquid drawn by pipe, pipe lower end is stretched into along qualification tube wall, the suction blister cavities extruding overcoat suction nozzle gently discharges bacterium liquid, quantitative calibrated pipet is slowly extracted out while extruding overcoat suction nozzle, make air return in pipe out, it is more even that bacterium liquid mixes with substratum.
3.2 contents are semi-solid or inclined-plane:
Use the quantitative graduated tube of oblique sharp opening, often 5-10 μ L bacterium liquid drawn by pipe, and Direct perforantes is inoculated.
4. cultivate:
After inoculation, biochemical identification pipe is concentrated and is placed in little beaker, then seal beaker mouth with a tinfoil or kraft paper, put thermostat container 36 ± 1 DEG C cultivation.
Last it is noted that the foregoing is only preferred embodiment of the present utility model; be not limited to the utility model; for a person skilled in the art; it still can regulate the technical scheme described in foregoing embodiments; or equivalent replacement is carried out to wherein portion of techniques feature; all within spirit of the present utility model and principle, any amendment done, equivalent replacement, improvement etc., all should be included within protection domain of the present utility model.
Claims (9)
1. one kind pipettes the quantitative graduated tube of solution for trace, comprise suction pipe and overcoat suction nozzle, it is characterized in that: also comprise connection deck, described suction pipe is capillary pipet, described Connection Card cover for seat is located at the top of suction pipe and is fixed with one with suction pipe, and described overcoat suction nozzle is set in and connects on deck, and connection deck is provided with multi-turn for strengthening the bulge loop of sealing effectiveness with the combination section of overcoat suction nozzle, in overcoat suction nozzle, be provided with suction blister cavities, described suction blister cavities is communicated with suction pipe.
2. a kind of quantitative graduated tube pipetting solution for trace according to claim 1, is characterized in that: described suction pipe be connected deck and make by transparent polymer material.
3. a kind of quantitative graduated tube pipetting solution for trace according to claim 2, is characterized in that: the outer tube wall of described suction pipe is provided with scale marks.
4. a kind of quantitative graduated tube pipetting solution for trace according to claim 3, is characterized in that: the minimum division value of described scale marks is 5 μ L.
5. a kind of quantitative graduated tube pipetting solution for trace according to claim 1-4 any one, it is characterized in that: described suction pipe outer tube diameter is 1-1.2mm, thickness of pipe is 0.2mm.
6. a kind of quantitative graduated tube pipetting solution for trace according to claim 5, is characterized in that: the lower port of described suction pipe is oblique sharp opening.
7. a kind of quantitative graduated tube pipetting solution for trace according to claim 5, is characterized in that: the lower port of described suction pipe is flat mouth, and the tube wall of suction pipe is provided with two side openings near lower port place.
8. a kind of quantitative graduated tube pipetting solution for trace according to claim 1-4 any one, it is characterized in that: described overcoat suction nozzle is made up of silica gel, overall in warhead form, the center of overcoat suction nozzle is inverted U-shaped open cavity, and described suction blister cavities is set in connect on deck by the U-shaped open cavity of overcoat suction nozzle to be formed with bulge loop close fit.
9. a kind of quantitative graduated tube pipetting solution for trace according to claim 1-4 any one, it is characterized in that: described connection deck is hollow circuit cylinder body structure, suction pipe be plugged on connect the center of deck and sidewall be connected deck and be compressed to integrally, the end face of suction pipe is concordant with the upper surface being connected deck.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520322885.7U CN204661699U (en) | 2015-05-19 | 2015-05-19 | A kind of quantitative graduated tube pipetting solution for trace |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520322885.7U CN204661699U (en) | 2015-05-19 | 2015-05-19 | A kind of quantitative graduated tube pipetting solution for trace |
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| Publication Number | Publication Date |
|---|---|
| CN204661699U true CN204661699U (en) | 2015-09-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520322885.7U Expired - Fee Related CN204661699U (en) | 2015-05-19 | 2015-05-19 | A kind of quantitative graduated tube pipetting solution for trace |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106226127A (en) * | 2016-09-25 | 2016-12-14 | 东莞市联洲知识产权运营管理有限公司 | A kind of sampling mechanism of microorganism |
-
2015
- 2015-05-19 CN CN201520322885.7U patent/CN204661699U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106226127A (en) * | 2016-09-25 | 2016-12-14 | 东莞市联洲知识产权运营管理有限公司 | A kind of sampling mechanism of microorganism |
| CN106226127B (en) * | 2016-09-25 | 2018-09-18 | 浙江富胜达科技有限公司 | A kind of sampling mechanism of microorganism |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150923 Termination date: 20190519 |