CN203474801U

CN203474801U - LAMP (Loop-Mediated Isothermal Amplification) reaction suite

Info

Publication number: CN203474801U
Application number: CN201320574833.XU
Authority: CN
Inventors: 周赞虎; 王根芳; 何王福; 林俊君; 何艺珍; 陈春香; 苏雪勤
Original assignee: ZHANGZHOU ENTRY-EXIT INSPECTION & QUARANTINE BUREAU OF PRC
Current assignee: ZHANGZHOU ENTRY-EXIT INSPECTION & QUARANTINE BUREAU OF PRC
Priority date: 2013-09-05
Filing date: 2013-09-05
Publication date: 2014-03-12
Anticipated expiration: 2023-09-05

Abstract

The utility model relates to an LAMP (Loop-Mediated Isothermal Amplification) reaction suite which comprises a reaction tube body and a syringe, wherein a silica gel stopper is arranged at the tube orifice of the reaction tube body, a syringe needle head is arranged at the lower end of a tube body of the syringe and pierces the silica gel stopper, and the silica gel stopper consists of an in-tube gel cover clung to the inner wall of the reaction tube body, and a gel riser clung to the tube orifice of the reaction tube body. According to the LAMP reaction tube, a material of a tube cover is changed into silica gel, after the LAMP reaction is completed, dyes can be sucked through a disposable sterile syringe, the silica gel stopper can be easily pieced by the disposable syringe, the dyes are added into reaction liquor; the LMIA reaction suite is simple in structure, a plastic cover of the reaction tube can be changed into the silica gel cover, so that the production is convenient; the commercial disposable sterile syringe is matched, the problem that fluorescent dyes influence the LAMP reaction can be thoroughly solved, and the use is convenient, and the production cost is low.

Description

A kind of loop-mediated isothermal amplification external member

Technical field

The utility model relates to reaction unit technical field, particularly relates to a kind of loop-mediated isothermal amplification external member.

Background technology

Ring mediated isothermal amplification (Loop-mediated isothermal amplification, LAMP) can be special under constant temperature, efficiently, DNA amplification rapidly.Because it does not need expensive instrument, easy, fast and specificity is good, susceptibility is high, in Micro biological Tests, transgenosis, identify and multi-field the obtaining such as disease detection studied widely and applied at present.The mensuration of LAMP amplification is mainly contained to nephelometry, fluorescence dye, gel electrophoresis and the large class of biosensor method four, and wherein gel electrophoresis and biosensor method be because need extra plant and instrument, operates also relatively loaded down with trivial detailsly, applies less; And nephelometry relative out of true aspect result judgement, so also apply now less; Fluorescence dye method does not need additional instruments, and result judges by the colour-change of fluorescence dye, accurately sensitive, and application at present at most.

LAMP fluorescence dye method, because production concentration is very high, easily causes environmental pollution, so relevant LAMP test kit tends to disposablely in a reaction tubes add all reagent to react; Although do like this, can avoid environmental pollution, fluorescence dye can produce and disturb LAMP reaction.

CN202808796U (a kind of loop-mediated isothermal amplification pipe) has disclosed a kind of novel loop-mediated isothermal amplification pipe, this invention is mainly provided with for placing the cavity of reagent at the bottom surface of pipe lid, and the bottom surface of described pipe lid is provided with for puncturing the sharp-pointed body of described cavity over against described cavity place.This invention has solved the problem (impact of fluorescence dye on LAMP reaction) of LAMP fluorescence dye method, but this invented technology relative complex, and require, in reaction tubes manufacturing processed, nucleic acid dye is directly put into cavity, once shaped, production cost is higher.

Summary of the invention

The purpose of this utility model is in order to overcome the deficiencies in the prior art, a kind of loop-mediated isothermal amplification external member is provided, solution LAMP fluorescence dye method exists fluorescence dye to add problem, and (LAPM reaction finishes the easy contaminate environment of rear interpolation fluorescence dye, LAPM reaction starts normally carrying out of front interpolation fluorescence dye impact reaction), production cost is low, easy to use simultaneously.

The utility model is achieved through the following technical solutions: a kind of loop-mediated isothermal amplification external member, comprise reaction body and syringe, described reaction tube mouth is provided with silica gel plug, the body lower end of described syringe is provided with injection needles, described injection needles pierces through silica gel plug, and described silica gel plug emits and forms with the glue that is adjacent to reaction tube mouth outer wall by being adjacent to gum cover in the pipe that reacts inboard wall of tube body.

Further, reaction body lower end is up big and down small conical end.

Further, described syringe is disposable sterilized injector.

Compared with prior art, the beneficial effects of the utility model are: it is silica gel that loop-mediated isothermal amplification pipe of the present utility model is managed cover material by change, after LAMP has reacted, can draw dyestuff by disposable sterilized injector, disposable aspiration needle is easy to pierce through silica gel plug, add dyestuff in reaction solution, while simple structure, only the plastic closure of reaction tubes is changed into silica gel lid, convenient for production, coordinate commercial disposable sterilized injector, thoroughly solve the affect problem of fluorescence dye on LAMP reaction, easy to use, low production cost.

Accompanying drawing explanation

Fig. 1 is the structural representation of a kind of loop-mediated isothermal amplification external member of the utility model.

Embodiment

In order to make the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the utility model is further elaborated.Should be appreciated that specific embodiment described herein is only in order to explain the utility model, and be not used in restriction the utility model.

As shown in Figure 1, the utility model relates to a kind of loop-mediated isothermal amplification external member, comprise reaction body 1 and syringe 4, described reaction body 1 mouth of pipe is provided with silica gel plug 2, the body lower end of described syringe 4 is provided with injection needles 3, described injection needles 3 pierces through silica gel plug 2, described silica gel plug 2 emits 6 to form by being adjacent to gum cover 7 in the pipe that reacts body 1 inwall with the glue that is adjacent to reaction body 1 mouth of pipe outer wall, reaction body 1 lower end is up big and down small conical end 5, and described syringe 4 is disposable sterilized injector.

In sum, the utility model simple structure, reacts the plastic closure of body 1 and changes into silica gel plug 2 by handle, facilitated production, good airproof performance, coordinates commercial disposable sterilized injector 4 simultaneously, thoroughly solves the affect problem of fluorescence dye on LAMP reaction, easy to use, low production cost, during use, draws dyestuff by disposable sterilized injector 4, and the body lower end injection needles 3 of syringe 4 is pierced through to silica gel plug 2, dyestuff is expelled to the reaction solution of reaction body 1.

More than show and described ultimate principle of the present utility model and principal character and advantage of the present utility model.The technician of the industry should understand; the utility model is not restricted to the described embodiments; that in above-described embodiment and specification sheets, describes just illustrates principle of the present utility model; do not departing under the prerequisite of the utility model spirit and scope; the utility model also has various changes and modifications, and these changes and improvements all fall within the scope of claimed the utility model.The claimed scope of the utility model is defined by appending claims and equivalent thereof.

Claims

1. a loop-mediated isothermal amplification external member, comprise reaction body (1) and syringe (4), it is characterized in that, described reaction body (1) mouth of pipe is provided with silica gel plug (2), the body lower end of described syringe (4) is provided with injection needles (3), described injection needles (3) pierces through silica gel plug (2), and described silica gel plug (2) emits (6) to form by being adjacent to the glue that reacts the interior gum cover (7) of pipe of body (1) inwall and be adjacent to reaction body (1) mouth of pipe outer wall.

2. a kind of loop-mediated isothermal amplification external member according to claim 1, is characterized in that, reaction body (1) lower end is up big and down small conical end (5).

3. a kind of loop-mediated isothermal amplification external member according to claim 1, is characterized in that, described syringe (4) is disposable sterilized injector.