CN205803496U - A kind of enzyme assay instrument - Google Patents
A kind of enzyme assay instrument Download PDFInfo
- Publication number
- CN205803496U CN205803496U CN201620769086.9U CN201620769086U CN205803496U CN 205803496 U CN205803496 U CN 205803496U CN 201620769086 U CN201620769086 U CN 201620769086U CN 205803496 U CN205803496 U CN 205803496U
- Authority
- CN
- China
- Prior art keywords
- test tube
- syringe
- enzyme assay
- support
- assay instrument
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of enzyme assay instrument, it is adaptable to effectively carrying out PH affects the experimental demonstration of enzymatic activity rapidly.This enzyme assay instrument is by including test tube support, syringe, test tube, and described test tube is inserted on the lower floor support of test tube support；Described syringe is inserted in the upper layer bracket of test tube support, and its syringe needle inserts on plug piston at the test tube mouth of pipe, and the upper end of the both sides side arm of described test tube support offers cylindrical hole, and the side arm on material-feeding rack both sides stretches in the hole being provided with spring just.Promoting needle tubing to move by reactant reaction in test tube, when reaction terminating, the curve shape observing needle tubing formation understands the process of reaction.
Description
Technical field
This utility model relates to a kind of bioconversion medium test and determines instrument, and being applicable to carry out PH particularly to one affects enzymatic activity
The enzyme assay instrument of experimental demonstration.
Technical background
" PH impact on enzymatic activity " is that people teaches an edition teaching material " biological required 1 molecule and cell " the 5th chapter Section 1 " enzyme
Feature " one joint in inquiry experiment, learn this experiment well, three big characteristics of enzyme can not only be better understood from, after being also study
The important contents such as ATP, photosynthesis, Cellular respiration have established solid basis.But, people teaches edition teaching material for this experiment only
Enzymatic activity is affected schematic diagram by PH, does not has concrete experimental provision and operating procedure, although the teaching material of some version gives experiment
Device and operating procedure, but the most complicated, and the most bad operation, success rate is the most extremely low.
Utility model content
For above-mentioned problems of the prior art and defect, the purpose of this utility model is to provide a kind of enzymatic activity
Analyzer, this analyzer is the most simple to operate, and can directly, vivo embody the curve that enzymatic activity is affected by PH.
Realize above-mentioned utility model purpose and be the technical scheme is that a kind of enzyme assay instrument, including test tube support,
Syringe, test tube, described test tube is inserted on the lower floor support of test tube support；Described syringe is inserted in the upper layer bracket of test tube support
On, its syringe needle inserts on plug piston at the test tube mouth of pipe, and the upper end of the both sides side arm of described test tube support offers cylinder
Hole, the side arm on material-feeding rack both sides stretches in the hole being provided with spring just.
Described syringe and a test tube row symmetrical above and below are provided with several.
The operating procedure of enzyme assay instrument of the present utility model: take 7 clean small beakers, be separately added into the mistake of equivalent
Hydrogen oxide enzymatic solution, is adjusted to 4,5,6,7,8,9,10 by its PH respectively by Hcl solution and NaOH solution；Take 7 clean examinations
Pipe, numbering 4,5,6,7,8,9,10 respectively, it is placed in order on the lower floor support of test tube rack, is separately added into 10ml30%'s to it
H2O2Solution, with rubber closure ferrule；Take 7 clean syringes, draw respectively the PH that 10ml configures be 5,6,7,8,
The Catalase solution of 9, is placed in the upper layer bracket of test tube rack, and the syringe equipped with different PH reaction reagent is inserted respectively
In the upper layer bracket of test tube support, the most respectively in the rubber closure of the syringe needle side of the being inserted under test tube of syringe, then will
Material-feeding rack catches, and preparation completes.Material-feeding rack is pressed downwards by final force, and the reagent in syringe is all noted
Entering in test tube, unclamp hands, material-feeding rack is bounced by spring.Reaction starts, the extruding of the gas that needle tubing is caused due to reaction
Acting on and start slowly to lift, due to reaction difference, the height lifted is the most different, thus forms a reaction mathematics song intuitively
Line.
Enzyme assay instrument operation principle of the present utility model: hydrogen peroxide can quickly produce under catalatic catalysis
Raw a large amount of oxygen, and most of enzyme is protein, therefore the activity of enzyme is affected the most notable by environmental PH.The most various
Enzyme only just shows its activity in the range of certain PH.Show its catalysis activity the highest time pH value be referred to as the suitableeest of this enzyme
PH, during below or above optimal pH, the activity of enzyme is gradually lowered.Thus can according in the identical time produce oxygen number
Judge the size of catalase activity.Following device oxygen produces the most, and air pressure is the biggest, and syringe ascensional range is the biggest, directly
Connect and can reflect such as " enzymatic activity is affected schematic diagram by PH " curve.
Enzyme assay instrument of the present utility model compared with prior art, has the advantage that
(1) save the traditional experiment time greatly, and reduce experimental implementation difficulty, simplify laboratory operating procedures；
(2) solve the enzyme by different PH process to be simultaneously injected in reaction.
(3) can the flat-footed parabola observed in a device in mathematical coordinates.By abstract mathematical thinking shape
Elephant be rendered as it is observed that concrete model；
(4) multi-functional: to install, dismantle simply, when not doing this experiment, be segmented into two this enzymatic activitys of common test tube frame
Analyzer；Simple in construction, feature richness, it is simple to promote.
Accompanying drawing illustrates:
Fig. 1 is the structural representation of this utility model a kind of enzyme assay instrument；
Fig. 2 is the work sheet of this utility model a kind of enzyme assay instrument；
Fig. 3 is the result figure of this utility model a kind of enzyme assay instrument.
Detailed description of the invention:
Below in conjunction with the accompanying drawings the example that is embodied as of the present utility model is described in further detail.
The structural representation of Fig. 1 this utility model a kind of enzyme assay instrument, including test tube support 1, syringe 7, test tube
8, described test tube 8 is inserted on the lower floor support 2 of test tube support 1；Described syringe 7 is inserted in the upper layer bracket 5 of test tube support 1,
Its syringe needle inserts on plug piston at test tube 8 mouth of pipe, and the upper end of the both sides side arm 3 of described test tube support 1 offers cylinder
Hole, the side arm on material-feeding rack 6 both sides stretches in the hole being provided with spring 4 just, described syringe 7 and test tube 8 symmetrical above and below
Row is provided with several.
First the test tube 8 having added reaction substrate 30% hydrogenperoxide steam generator is placed on reactant fixed support 1, then will
The needle tubing 7 adding different PH process Catalase solution inserts the test tube 8 (mouth of pipe is plugged with rubber closure) of correspondence, is connected with test tube
It is integrally formed, conveniently enzymatic solution is added test tube.
Install material-feeding rack 6, extrude material-feeding rack (such as Fig. 2) so that the enzymatic solution in needle tubing 7 is simultaneously introduced test tube 8,
Reacting, produce oxygen, oxygen gas increases, and in test tube 7, pressure increases, and gas push needle tubing 8 piston moves, due to difference
The enzymatic activity that PH processes is different, and gas production rate is different, so in the unit interval, piston moving speed different.Make needle tubing
Piston presents a parabola (such as Fig. 3).
Claims (2)
1. an enzyme assay instrument, including test tube support (1), syringe (7), test tube (8), it is characterised in that described test tube
(8) it is inserted on the lower floor support (2) of test tube support (1)；Described syringe (7) is inserted in the upper layer bracket (5) of test tube support (1)
On, its syringe needle inserts on plug piston at test tube (8) mouth of pipe, and the upper end of the both sides side arm (3) of described test tube support (1) is offered
Cylindrical hole, the side arm on material-feeding rack (6) both sides is had just to stretch in the hole being provided with spring (4).
Enzyme assay instrument the most according to claim 1, it is characterised in that described syringe (7) and test tube (8) are the most right
A row is claimed to be provided with several.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620769086.9U CN205803496U (en) | 2016-07-13 | 2016-07-13 | A kind of enzyme assay instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620769086.9U CN205803496U (en) | 2016-07-13 | 2016-07-13 | A kind of enzyme assay instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205803496U true CN205803496U (en) | 2016-12-14 |
Family
ID=57509132
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620769086.9U Expired - Fee Related CN205803496U (en) | 2016-07-13 | 2016-07-13 | A kind of enzyme assay instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205803496U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106319027A (en) * | 2016-07-13 | 2017-01-11 | 高静 | Visualized enzyme activity determination method and determinator thereof |
| CN114985023A (en) * | 2021-10-27 | 2022-09-02 | 北京擎科生物科技有限公司 | Microarray chip and oligonucleotide synthesis apparatus including the same |
-
2016
- 2016-07-13 CN CN201620769086.9U patent/CN205803496U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106319027A (en) * | 2016-07-13 | 2017-01-11 | 高静 | Visualized enzyme activity determination method and determinator thereof |
| CN114985023A (en) * | 2021-10-27 | 2022-09-02 | 北京擎科生物科技有限公司 | Microarray chip and oligonucleotide synthesis apparatus including the same |
| CN114985023B (en) * | 2021-10-27 | 2024-01-05 | 北京擎科生物科技股份有限公司 | Microarray chip and oligonucleotide synthesizer comprising the same |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN205803496U (en) | A kind of enzyme assay instrument | |
| CN203474801U (en) | LAMP (Loop-Mediated Isothermal Amplification) reaction suite | |
| CN202558865U (en) | Biology sampling swab | |
| CN213611497U (en) | Flexible fixing device of biological enzyme solution test tube | |
| CN106319027A (en) | Visualized enzyme activity determination method and determinator thereof | |
| CN101182454A (en) | External-internal double-cycling bioreactor | |
| CN215050239U (en) | Bioreactor aseptic sampling kit | |
| CN201141027Y (en) | Inner and outer double-circulation bioreactor | |
| CN106475028B (en) | A kind of multifunctional bio engineering reactor | |
| CN201419102Y (en) | Biological culture bottle for experiment in middle school | |
| CN2603089Y (en) | Large bore diameter fermentation inoculator | |
| CN101928662A (en) | Edible fungi liquid strain biological fermentation tank | |
| CN2921835Y (en) | Blood culture bottle | |
| CN203938518U (en) | Nanometer aeration device | |
| CN202838785U (en) | Gas experiment micro environmental protection device | |
| CN217875293U (en) | Multi-channel vacuumizing and gas replacement portable device | |
| CN201379578Y (en) | Multifunctional needle tube | |
| CN104030434A (en) | Nanometer aeration device and nanometer aeration method | |
| CN203057903U (en) | Simple liquid strain inoculation device | |
| CN204495608U (en) | A kind of clinical laboratory Sputamentum sampler | |
| CN214300128U (en) | Loop-mediated isothermal amplification reaction tube based on capillary tube | |
| CN203159611U (en) | Shake flask facilitating sampling | |
| CN203593747U (en) | Minisize biological shaking table for cell suspending liquid inoculation | |
| CN201799291U (en) | Test-tube stand five-connection sleeve of biochemical analyzer | |
| CN203324290U (en) | Bacterial suspension sample injector |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161214 Termination date: 20170713 |