CN205839009U - A kind of loop-mediated isothermal amplification pipe - Google Patents
A kind of loop-mediated isothermal amplification pipe Download PDFInfo
- Publication number
- CN205839009U CN205839009U CN201620629783.4U CN201620629783U CN205839009U CN 205839009 U CN205839009 U CN 205839009U CN 201620629783 U CN201620629783 U CN 201620629783U CN 205839009 U CN205839009 U CN 205839009U
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- China
- Prior art keywords
- silica gel
- negative tube
- tube body
- syringe needle
- needle
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- Materials For Medical Uses (AREA)
Abstract
The utility model discloses a kind of loop-mediated isothermal amplification pipe, including silica gel plug, negative tube body, the first syringe needle, the soft ball of silica gel and piercing needle；Described silica gel plug is fixed on the top of negative tube body, and on silica gel plug, it is provided with recessed circle, it is fixed with projection on the top of described negative tube inner body wall, it is provided with graduation mark and mark sheet on the outer wall of described negative tube body, and graduation mark is arranged on the left side of mark sheet, the bottom of described negative tube body is provided with at the bottom of curved circular, on described first syringe needle and the second syringe needle, shell is installed, and first connect by flexible catheter is fixing between syringe needle and the second syringe needle, the bottom of the soft ball of described silica gel and piercing needle are by seal head compact siro spinning technology, it is installed with handle on described piercing needle.This utility model negative tube body, in the overall process used, uses aseptic injection, and solving fluorescent dye affects problem to what LAMP reacted, it is to avoid cross infection, and product is easy to use, low production cost.
Description
Technical field
This utility model relates to reaction unit technical field, is specially a kind of loop-mediated isothermal amplification pipe.
Background technology
Ring mediated isothermal amplification (Loop-mediated isothermal amplification, LAMP) can be at constant temperature
Under the conditions of special, efficiently, DNA amplification rapidly；Because it need not the instrument of costliness, easy, quickly and specificity is good, sensitive
Property high, the most identify and disease detection etc. is multi-field is studied widely and apply at Micro biological Tests, transgenic；Right
The mensuration of LAMP amplification mainly has nephelometry, fluorescent dye, gel electrophoresis and the big class of hexavalent chrome bio-removal four, Qi Zhongning
Gel electrophoresis and hexavalent chrome bio-removal, since it is desired that extra instrument and equipment, operate the most relatively cumbersome, apply less；And nephelometry exists
Result judgement aspect is relative to inaccuracy, so also applying now less；Fluorescent dye determination is not required to additional instruments, and result passes through fluorescence
The color change of dyestuff judges, the sensitiveest, at present application at most, but the addition of these dyestuffs all can more or less right
Response speed produces certain impact, for avoiding these to affect, adds dye after having researcher to take LAMP amplified reaction to terminate
Material, but so easily cause diffusion and the Aerosol Pollution of product, so that testing result false positive rate is high, and
And once pollute environment, it is difficult to, to remove, cause serious consequence.Therefore to solution problems of the prior art, existing
There is provided a kind of negative tube body in the overall process used, use aseptic injection, solve the impact that LAMP is reacted by fluorescent dye
Problem, it is to avoid cross infection, and product is easy to use, the loop-mediated isothermal amplification pipe of low production cost.
Summary of the invention
The purpose of this utility model is to provide a kind of loop-mediated isothermal amplification pipe, to solve in above-mentioned background technology
The problem proposed, is had an advantageous effect in that；Negative tube body, in the overall process used, uses aseptic injection, solves glimmering
Photoinitiator dye affects problem to what LAMP reacted, it is to avoid cross infection, and product is easy to use, low production cost.
For achieving the above object, this utility model following technical scheme of offer: a kind of loop-mediated isothermal amplification pipe, bag
Include silica gel plug, negative tube body, the first syringe needle, the soft ball of silica gel and piercing needle；Described silica gel plug is fixed on negative tube body
It is provided with recessed circle on top, and silica gel plug, the top of described negative tube inner body wall is fixed with projection, described negative tube body
Outer wall on be provided with graduation mark and mark sheet, and graduation mark is arranged on the left side of mark sheet, the bottom of described negative tube body
It is provided with at the bottom of curved circular, described first syringe needle and the second syringe needle is provided with between shell, and the first syringe needle and the second syringe needle logical
Crossing the fixing connection of flexible catheter, the bottom of the soft ball of described silica gel and piercing needle are by seal head compact siro spinning technology, described piercing needle
On be installed with handle.
Preferably, described silica gel plug is fixed by the compact siro spinning technology of protruding and recessed circle with negative tube body.
Preferably, described first syringe needle and silica gel plug compact siro spinning technology, and the bottom of the first syringe needle is arranged on negative tube body
Inside.
Preferably, described piercing needle and silica gel plug compact siro spinning technology, and the bottom of piercing needle is arranged on negative tube body
Inside.
Compared with prior art, the beneficial effects of the utility model are: the inside of this negative tube body is negative pressure cavity, adopts
With the first syringe needle, flexible catheter and the second needle tubing with the use of, it is ensured that in negative tube, the absorption of raw material and the process of injection are aseptic
Contact, solving fluorescent dye affects problem to what LAMP reacted, it is to avoid cross infection, the soft ball of silica gel sets with piercing needle
Put, it is ensured that the intrinsic pressure of negative tube is stable in course of reaction, overcome the problem that pressure is unstable, and the design of product
Rationally, easy to use, low production cost, be conducive to promoting the use of.
Accompanying drawing explanation
Fig. 1 is the structural representation of this utility model negative tube body；
Fig. 2 is the structural representation of this utility model negative tube liquid feeding；
Fig. 3 is the structural representation that this utility model inserts piercing needle；
Fig. 4 is the structural representation after this utility model inserts piercing needle.
In figure: 1-silica gel plug；2-negative tube body；3-graduation mark；4-is protruding；5-mark sheet；At the bottom of 6-curved circular；Outside 7-
Shell；8-the first syringe needle；9-flexible catheter；10-the second syringe needle；11-seal head；The soft ball of 12-silica gel；13-handle；14-piercing needle.
Detailed description of the invention
Below in conjunction with the accompanying drawing in this utility model embodiment, the technical scheme in this utility model embodiment is carried out
Clearly and completely describe, it is clear that described embodiment is only a part of embodiment of this utility model rather than whole
Embodiment.Based on the embodiment in this utility model, those of ordinary skill in the art are not under making creative work premise
The every other embodiment obtained, broadly falls into the scope of this utility model protection.
Refer to Fig. 1-4, a kind of embodiment that this utility model provides: a kind of loop-mediated isothermal amplification pipe, including
Silica gel plug 1, negative tube body the 2, first syringe needle 8, the soft ball of silica gel 12 and piercing needle 14；Silica gel plug 1 is fixed on negative tube body 2
Top, and on silica gel plug 1, be provided with recessed circle, the top of negative tube body 2 inwall be fixed with protruding 4, negative tube body 2
Being provided with graduation mark 3 and mark sheet 5 on outer wall, and graduation mark 3 is arranged on the left side of mark sheet 5, the bottom of negative tube body 2 sets
It is equipped with at the bottom of curved circular 6, the first syringe needle 8 and the second syringe needle 10 is provided with between shell 7, and the first syringe needle 8 and the second syringe needle 10
Connecting by flexible catheter 9 is fixing, the bottom of the soft ball of silica gel 12 and piercing needle 14 are by seal head 11 compact siro spinning technology, piercing needle
Being installed with handle 13 on 14, silica gel plug 1 is fixed by the compact siro spinning technology of protruding 4 with recessed circle with negative tube body 2, the first pin
8 with silica gel plug 1 compact siro spinning technology, and the bottom of the first syringe needle 8 is arranged on the inside of negative tube body 2, piercing needle 14 and silicon
Plug 1 compact siro spinning technology, and the bottom of piercing needle 14 is arranged on the inside of negative tube body 2.
Operation principle；During use, the first syringe needle 8 arrives the inside of negative tube body 2, negative tube body 2 through silica gel plug 1
The pressure within reagent bottle that internal pressure connects less than the second syringe needle 10, reaction reagent is arrived by the transmission of flexible catheter 9
The inside of negative tube body 2, takes out the first syringe needle 8, is inserted in negative tube body 2 through silica gel plug 1 by piercing needle 14
Portion, the soft ball of silica gel 12 can balance the pressure in negative tube body 2, after reaction terminates, by the first syringe needle 8 and the second syringe needle 10
With the use of, reacted reagent in negative tube body 2 can be transferred in holding bottle by flexible catheter 9.
It is obvious to a person skilled in the art that this utility model is not limited to the details of above-mentioned one exemplary embodiment, and
And in the case of without departing substantially from spirit or essential attributes of the present utility model, it is possible to realize this practicality in other specific forms new
Type.Therefore, no matter from the point of view of which point, all should regard embodiment as exemplary, and be nonrestrictive, this practicality is new
The scope of type is limited by claims rather than described above, it is intended that by the containing of equivalency in claim that fall
All changes in justice and scope are included in this utility model.Should not be considered as any reference in claim limiting
Involved claim.
Claims (4)
1. a loop-mediated isothermal amplification pipe is soft including silica gel plug (1), negative tube body (2), the first syringe needle (8), silica gel
Ball (12) and piercing needle (14)；It is characterized in that: described silica gel plug (1) is fixed on the top of negative tube body (2), and silica gel
It is provided with recessed circle on plug (1), the top of described negative tube body (2) inwall is fixed with projection (4), described negative tube body
(2) it is provided with graduation mark (3) and mark sheet (5) on outer wall, and graduation mark (3) is arranged on the left side of mark sheet (5), described negative
The bottom of pressure pipe body (2) is provided with (6) at the bottom of curved circular, and described first syringe needle (8) and the second syringe needle (10) are provided with shell
, and be connected by flexible catheter (9) is fixing between the first syringe needle (8) with the second syringe needle (10), the end of the soft ball of described silica gel (12) (7)
Portion and piercing needle (14), by seal head (11) compact siro spinning technology, described piercing needle (14) are installed with handle (13).
A kind of loop-mediated isothermal amplification pipe the most according to claim 1, it is characterised in that: described silica gel plug (1) with
Negative tube body (2) is fixed by the compact siro spinning technology of protruding (4) with recessed circle.
A kind of loop-mediated isothermal amplification pipe the most according to claim 1, it is characterised in that: described first syringe needle (8)
With silica gel plug (1) compact siro spinning technology, and the bottom of the first syringe needle (8) is arranged on the inside of negative tube body (2).
A kind of loop-mediated isothermal amplification pipe the most according to claim 1, it is characterised in that: described piercing needle (14)
With silica gel plug (1) compact siro spinning technology, and the bottom of piercing needle (14) is arranged on the inside of negative tube body (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620629783.4U CN205839009U (en) | 2016-06-24 | 2016-06-24 | A kind of loop-mediated isothermal amplification pipe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620629783.4U CN205839009U (en) | 2016-06-24 | 2016-06-24 | A kind of loop-mediated isothermal amplification pipe |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205839009U true CN205839009U (en) | 2016-12-28 |
Family
ID=57621166
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620629783.4U Expired - Fee Related CN205839009U (en) | 2016-06-24 | 2016-06-24 | A kind of loop-mediated isothermal amplification pipe |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205839009U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110331089A (en) * | 2019-05-21 | 2019-10-15 | 宁波迪亚生物科技有限公司 | A kind of full-automatic nucleic acid extraction augmentation detection micro-fluidic chip box and its application |
-
2016
- 2016-06-24 CN CN201620629783.4U patent/CN205839009U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110331089A (en) * | 2019-05-21 | 2019-10-15 | 宁波迪亚生物科技有限公司 | A kind of full-automatic nucleic acid extraction augmentation detection micro-fluidic chip box and its application |
| CN110331089B (en) * | 2019-05-21 | 2023-07-21 | 四川迪亚生物科技集团有限公司 | Full-automatic nucleic acid extraction amplification detection micro-fluidic chip box and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161228 Termination date: 20180624 |