CN102732419A - Object surface microbe sampling culture dish - Google Patents
Object surface microbe sampling culture dish Download PDFInfo
- Publication number
- CN102732419A CN102732419A CN2012101904004A CN201210190400A CN102732419A CN 102732419 A CN102732419 A CN 102732419A CN 2012101904004 A CN2012101904004 A CN 2012101904004A CN 201210190400 A CN201210190400 A CN 201210190400A CN 102732419 A CN102732419 A CN 102732419A
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- China
- Prior art keywords
- wall
- petridish
- molectron
- culture dish
- round platform
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to an object surface microbe sampling culture dish. The culture dish comprises a round table pedestal 1, a composite unit outer wall 2, a culture dish cover 3, and a composite outer wall protrusion 4. The round table pedestal 1 and the composite unit outer wall 2 movably connected therewith form a culture dish body. The culture dish body is tightly connected with the friction force of the materials and effects of interference fit and geometric self-locking. The composite outer wall protrusion 4 is used for supporting the culture dish cover 3, such that the upper bottom of the culture dish cover 3 is prevented from contacting a culture medium. With the composition and connection method provided by the invention, the tightness and sterility of the culture dish are ensured. The culture dish provided by the invention is advantaged in simple structure and convenient application. With the culture dish, object surface microbe sampling and culturing steps are simplified, and the tightness and sterility of the culture dish are ensured. During a sampling process, contamination bacterium infection is prevented, and the object surface microbe detection sampling efficiency and sample result accuracy are improved.
Description
Technical field
The present invention relates to a kind of body surface microbe sampling petridish.This petridish can carry out direct sampling to the body surface mikrobe, begins immediately after the sampling to cultivate, and has omitted the transfer process of microbiological specimens, has improved the sampling efficiency of body surface microorganism detection and the accuracy rate of sample result.
Background technology
Microbe sampling relates to every field such as medical treatment, food sanitation, pharmaceutical industry, aerospace, and the wherein extremely important especially aspect of body surface microbe sampling.At present, for the microbe sampling of body surface, mainly be with antiseptic swab wiping object under test surface, the back swab of will sampling again is transferred to wash-out in the damping fluid, elutriant is transferred in the solid medium cultivates then.Yet the best antiseptic swab of effect also only has 90% to the release rate of mikrobe, can cause the loss of microbiological specimens unavoidably; And this operating process is loaded down with trivial details; Time is slow, very easily microbiological contamination, and these shortcomings all can cause than mistake the microorganism count of body surface to be measured.Therefore, how to simplify body surface microbe sampling process and just become key.
Summary of the invention
To above-mentioned shortcoming, the present invention proposes a kind of body surface microbe sampling petridish, this petridish comprises round platform base 1; Molectron outer wall 2, petridish lid 3, molectron outer wall protruding 4; Round platform base 1 and the molectron outer wall 2 that flexibly connects are with it formed petridish ware body; Utilize the frictional force of material itself, and utilize shrink-fit and how much self-lockings to realize being fastenedly connected of ware body, molectron outer wall protruding 4 is used for supporting petridish lid 3; The upper base that prevents petridish lid 3 touches substratum, and the aforesaid combination mode of connection has all guaranteed the stopping property and the sterility of petridish.
This petridish adopts transparent and the organic composite macromolecular material of the corrosion-and high-temp-resistant of certain degree of hardness is arranged, and the following internal diameter of round platform base 1 is 3-10cm, and last internal diameter is 4-11cm; Highly be 1-2cm; The last internal diameter of molectron outer wall 2 is 5-12cm, and the whole height of molectron outer wall 2 is 1-5cm, and the frustum cone height of molectron outer wall 2 is greater than or equals the height of round platform base 1; Guarantee top external diameter seamless being fastenedly connected when stretching of the following internal diameter and the round platform base 1 of molectron outer wall 2; The thickness of molectron outer wall protruding 4 is 0.1-0.2cm, and the internal diameter of petridish lid 3 is greater than the edge external diameter 0.1-0.2cm of molectron outer wall 2, and the height of petridish lid 3 is 1-2cm.
Major advantage of the present invention is; Simple in structure; Convenient to use, simplify the step that the body surface microbe sampling is cultivated, and guaranteed the stopping property and the sterility of petridish; In sampling process, be difficult for bacteria infection, improved the sampling efficiency of body surface microorganism detection and the accuracy rate of sample result.
Description of drawings
Front view when Fig. 1 stretches for apparatus of the present invention.
Front view when Fig. 2 shrinks for apparatus of the present invention.
Fig. 3 is the vertical view of apparatus of the present invention.
Among the figure: the 1st, the round platform base, the 2nd, the molectron outer wall, the 3rd, the petridish lid, the 4th, the molectron outer wall is protruding.
Embodiment
Below in conjunction with specific examples and Figure of description the present invention is further specified.The present invention's scope required for protection is not limited to the scope described in the embodiment.
A kind of body surface microbe sampling petridish as depicted in figs. 1 and 2 comprises round platform base 1, molectron outer wall 2; Petridish lid 3; Molectron outer wall convexity 4, round platform base 1 is formed petridish ware bodies with the molectron outer wall that flexibly connects with it 2, utilizes the frictional force of material itself; And utilize shrink-fit and how much self-lockings to realize being fastenedly connected of ware body; Molectron outer wall protruding 4 is used for supporting petridish lid 3, prevents that the upper base of petridish lid 3 from touching substratum, and the aforesaid combination mode of connection has all guaranteed the stopping property and the sterility of petridish.
This body surface microbe sampling petridish behind the sterilization is opened lid in the aseptic technique platform; And this petridish molectron outer wall 2 stretched out; Like Fig. 1, will be autoclaved substratum inject this petridish, half the to molectron outer wall 2 cylinder heights; Cover petridish lid 3, wait for culture medium solidifying.
Treat to take a sample after the substratum condensation, with 2 withdrawals of molectron outer wall, like Fig. 2, form a protruding media surface this moment, is pressed in object under test surface, direct sampling gently with this protruding media surface during sampling.Immediately molectron outer wall 2 is stretched out after the sampling,, cover substratum lid 3, put under the suitable condition and cultivate like Fig. 1.
Claims (2)
1. body surface microbe sampling petridish, it is characterized in that: this petridish comprises round platform base 1, molectron outer wall 2; Petridish lid 3; Molectron outer wall convexity 4, round platform base 1 and the molectron outer wall 2 that flexibly connects are with it formed petridish ware bodies, utilize the frictional force of material itself; And utilize shrink-fit and how much self-lockings to realize being fastenedly connected of ware body; Molectron outer wall protruding 4 is used for supporting petridish lid 3, prevents that the upper base of petridish lid 3 from touching substratum, and the aforesaid combination mode of connection has all guaranteed the stopping property and the sterility of petridish.
2. a kind of body surface microbe sampling petridish according to claim 1; It is characterized in that: this petridish adopts transparent and the organic composite macromolecular material of the corrosion-and high-temp-resistant of certain degree of hardness is arranged, and the following internal diameter of round platform base 1 is 3-10cm, and last internal diameter is 4-11cm; Highly be 1-2cm; The last internal diameter of molectron outer wall 2 is 5-12cm, and the whole height of molectron outer wall 2 is 1-5cm, and the frustum cone height of molectron outer wall 2 is greater than or equals the height of round platform base 1; Guarantee top external diameter seamless being fastenedly connected when stretching of the following internal diameter and the round platform base 1 of molectron outer wall 2; The thickness of molectron outer wall protruding 4 is 0.1-0.2cm, and the internal diameter of petridish lid 3 is greater than the edge external diameter 0.1-0.2cm of molectron outer wall 2, and the height of petridish lid 3 is 1-2cm.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN2012101904004A CN102732419B (en) | 2012-06-08 | 2012-06-08 | Object surface microbe sampling culture dish |
Applications Claiming Priority (1)
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CN2012101904004A CN102732419B (en) | 2012-06-08 | 2012-06-08 | Object surface microbe sampling culture dish |
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CN102732419A true CN102732419A (en) | 2012-10-17 |
CN102732419B CN102732419B (en) | 2013-11-13 |
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CN2012101904004A Expired - Fee Related CN102732419B (en) | 2012-06-08 | 2012-06-08 | Object surface microbe sampling culture dish |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104293657A (en) * | 2014-11-10 | 2015-01-21 | 莆田学院 | Sealed culture dish |
CN106635765A (en) * | 2016-11-07 | 2017-05-10 | 天津施特雷生物科技股份有限公司 | Disposable sterile microbial culture dish |
CN108603158A (en) * | 2016-02-08 | 2018-09-28 | 贝克顿·迪金森公司 | Preparative plating medium product |
CN111733204A (en) * | 2020-07-03 | 2020-10-02 | 安徽屾远材料技术有限公司 | Microbial rapid detection membrane and preparation method and use method thereof |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0915152A2 (en) * | 1997-10-06 | 1999-05-12 | Becton, Dickinson and Company | Microorganism sampling device |
US20010024821A1 (en) * | 1999-12-09 | 2001-09-27 | Potter Steve M. | Sealed culture chamber |
CN2652938Y (en) * | 2003-09-10 | 2004-11-03 | 芦森 | Anaerobe cultivation medium |
CN102199528A (en) * | 2011-03-08 | 2011-09-28 | 北京航空航天大学 | Device for sampling microorganisms from surface of object |
CN202643697U (en) * | 2012-06-08 | 2013-01-02 | 北京航空航天大学 | Object surface microbe sampling culture dish |
-
2012
- 2012-06-08 CN CN2012101904004A patent/CN102732419B/en not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0915152A2 (en) * | 1997-10-06 | 1999-05-12 | Becton, Dickinson and Company | Microorganism sampling device |
US20010024821A1 (en) * | 1999-12-09 | 2001-09-27 | Potter Steve M. | Sealed culture chamber |
CN2652938Y (en) * | 2003-09-10 | 2004-11-03 | 芦森 | Anaerobe cultivation medium |
CN102199528A (en) * | 2011-03-08 | 2011-09-28 | 北京航空航天大学 | Device for sampling microorganisms from surface of object |
CN202643697U (en) * | 2012-06-08 | 2013-01-02 | 北京航空航天大学 | Object surface microbe sampling culture dish |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104293657A (en) * | 2014-11-10 | 2015-01-21 | 莆田学院 | Sealed culture dish |
CN108603158A (en) * | 2016-02-08 | 2018-09-28 | 贝克顿·迪金森公司 | Preparative plating medium product |
CN106635765A (en) * | 2016-11-07 | 2017-05-10 | 天津施特雷生物科技股份有限公司 | Disposable sterile microbial culture dish |
CN111733204A (en) * | 2020-07-03 | 2020-10-02 | 安徽屾远材料技术有限公司 | Microbial rapid detection membrane and preparation method and use method thereof |
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CN102732419B (en) | 2013-11-13 |
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