CN102465095A - Radioactive gas collection cell culture apparatus - Google Patents
Radioactive gas collection cell culture apparatus Download PDFInfo
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- CN102465095A CN102465095A CN2010105306636A CN201010530663A CN102465095A CN 102465095 A CN102465095 A CN 102465095A CN 2010105306636 A CN2010105306636 A CN 2010105306636A CN 201010530663 A CN201010530663 A CN 201010530663A CN 102465095 A CN102465095 A CN 102465095A
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- cell culture
- radioactive gas
- bottleneck
- culture apparatus
- tissue culture
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Abstract
The invention relates to a radioactive gas collection cell culture apparatus. The apparatus comprises a cell culture bottle and is characterized in that: a radioactive medium is injected into the culture bottle, and a seal assembly is arranged at the bottleneck of the cell culture bottle; an injection means used for terminating a metabolic reaction of cells is connected with the cell culture bottle; the cell culture bottle is connected with a scintillation flask through a transmission assembly, the bottleneck of the scintillation flask is also provided with a seal assembly, and absorption liquid is provided in the scintillation flask. According to the invention, each assembly can be modularly assembled, the apparatus is directed at the demand for disposable usage in a radioactive experiment, and each used assembly can be replaced at any time, thereby effectively lowering down cost, reducing the volume of waste and meeting demands for disinfection; meanwhile, the total volume of the apparatus is small, culture space is saved, and the apparatus is suitable for batch culture, can meet requirements for batch experiments and can be used in the whole process from generation of radioactive gas to collection and measurement of the radioactive gas.
Description
Technical field
The present invention relates to a kind of culture apparatus, relate in particular to a kind of radioactive gas collecting cell culture apparatus.
Background technology
As shown in Figure 2; Existing cell culture apparatus effect is the radioactivity carbonic acid gas that the collecting cell metabolism produces in an airtight environment; Intermediary pillar at the bottom of its bottle; Be a upholder, effect is the columniform pipe above supporting, and adds the collection liquid hydrogen oxidation quaternary amine of carbonic acid gas in the pipe.
But existing cell culture apparatus has following shortcoming:
1, be difficult for to cultivate attached cell, it is difficult for adding cell suspension, be difficult for changing liquid, nondigestible cell and go down to posterity.
2, volume is too big, is difficult for putting into incubator and cultivates in batches, is difficult for satisfying experimental requirements in batches.
3, apparatus structure is comparatively complicated, is difficult for reaching the required aseptic requirement of cell cultures, and sterilization, handling process are damaged device feature easily.
4, the liquid that needs to have collected radioactive gas is transferred to scintillation vial, has increased operation steps, thereby has increased radioactive substance loss and the risk of polluting.
5, apparatus structure is comparatively complicated, is difficult for batch process, thereby has increased the cost of one-piece apparatus.
6, device volume is bigger, as radioactivity experiment, disposable use, has correspondingly increased the volume of radwaste.
Summary of the invention
The object of the invention is exactly the problems referred to above that exist in the prior art in order to solve, and a kind of radioactive gas collecting cell culture apparatus is provided.
The object of the invention is realized through following technical scheme: a kind of radioactive gas collecting cell culture apparatus includes Tissue Culture Flask, wherein: be injected with the radioactivity substratum in the said Tissue Culture Flask; Described Tissue Culture Flask bottleneck is provided with seal assembly; Be connected with the injection device that is used to stop the cellular metabolism reaction on the described Tissue Culture Flask; Described Tissue Culture Flask is connected into scintillation vial through transmission assembly; Described scintillation vial bottleneck is provided with seal assembly, is provided with absorption liquid in the described scintillation vial.
Above-mentioned a kind of radioactive gas collecting cell culture apparatus, wherein: described radioactivity substratum contains 14C-glucose, and its content can be 0.1～2 μ Ci.
Further, above-mentioned a kind of radioactive gas collecting cell culture apparatus, wherein: described injection device is an injector for medical purpose, injected material is H
2SO
4
Further, above-mentioned a kind of radioactive gas collecting cell culture apparatus, wherein: described transmission assembly is a disposal vacuum blood sampling conduit, described disposal vacuum blood sampling conduit two ends are provided with threads a needle.
Again further, above-mentioned a kind of radioactive gas collecting cell culture apparatus, wherein: described seal assembly includes turned welt latex plug, and described turned welt latex plug hoop is in the bottleneck outer of Tissue Culture Flask, scintillation vial; Turned welt latex and bottleneck interface are wound with and seal film.
The advantage of technical scheme of the present invention is mainly reflected in: the assembling that each assembly all can composition moduleization; Need disposable use to the radioactivity experiment; Can replace the assembly that each has used at any time, not only effectively reduce cost but also reduced the refuse volume, satisfy the sterilization demand simultaneously.And overall volume of the present invention is little, practices thrift culture space, is fit to cultivate in batches, can satisfy experimental requirements in batches.Moreover volume of the present invention is less, utilizes the present invention can realize the whole process that radioactive gas is collected, measured from producing to.
Description of drawings
The object of the invention, advantage and characteristics will illustrate through the non-limitative illustration of following preferred embodiment and explain.These embodiment only are the prominent examples of using technical scheme of the present invention, and all technical schemes of taking to be equal to replacement or equivalent transformation and forming all drop within the scope of requirement protection of the present invention.In the middle of these accompanying drawings,
Fig. 1 is the enforcement synoptic diagram of this radioactive gas collecting cell culture apparatus.
Fig. 2 is the organigram of existing culture apparatus.
Among the figure
1 Tissue Culture Flask, 2 substratum
3 scintillation vials, 4 absorption liquids
5 injector for medical purpose, 6 vacuum blood collection conduits
7 seal film 8 turned welt latex plugs
Embodiment
A kind of radioactive gas collecting cell culture apparatus as shown in Figure 1 includes Tissue Culture Flask 1, and its unusual part is: be injected with radioactivity substratum 2 in the Tissue Culture Flask 1 that the present invention adopts, be provided with seal assembly at Tissue Culture Flask 1 bottleneck.Simultaneously, on Tissue Culture Flask 1, be connected with the injection device that is used to stop the cellular metabolism reaction.And Tissue Culture Flask 1 is connected into scintillation vial 3 through transmission assembly, and these scintillation vial 3 bottlenecks also are provided with seal assembly, are provided with absorption liquid 4 in the described scintillation vial 3.Thus, constituted a modular culture apparatus.
With regard to the present invention's one preferred implementation, consider radioactivity tracking convenience, the radioactivity substratum 2 that the present invention adopts contains
14C-glucose, its content can be 0.1～2 μ Ci.Specifically, the big more radioactivity of activity is strong more, and the radiocounting that measures at last is also many more, but has equally also increased the radioactive intensity of radwaste, has increased the cost of waste treatment.So in order to realize general experiment, the activity of 0.1～2 μ Ci should satisfy, low excessively activity then possibly cause radiocounting low excessively, and then causes the difference with insignificance of experimental group and control group and false negative (result's) consequence occurs.
Further, in order in the very first time, to inject relevant termination material, the injection device that the present invention adopts is an injector for medical purpose 5, and injected material is H
2SO
4, and H
2SO
4Content be 4～10mol/L.Specifically; Here use the purpose of injector for medical purpose 5 to be: syringe has needle point, under the prerequisite of not destroying whole device stopping property, liquid is injected culturing bottle at the turned welt latex plug 8 of bottleneck thereby can thrust hoop, is the stopping property of whole device because test most important; If gas leakage; To cause the excessive of radioactive gas, not only cause radiocontamination, the more important thing is seriously influencing experimental result.
The use of injected material: the substratum 2 that culturing cell is used is alkaline; And we need measure is the amount of radioactivity carbonic acid gas; Identical with inactive normal carbonic acid gas; The radioactivity carbonic acid gas also is a tart gas, so the carbonic acid gas that cell is breathed out can be absorbed by alkaline medium on every side 2.That is to say that carbonic acid gas is can get into scintillation vial 3 and by the liquid-absorbent in the scintillation vial 3 through conduit hardly before stopping metabolic reaction annotating acid.We inject the vitriolic purpose just for the carbonic acid gas that is dissolved in alkaline liq is discharged, under the airtight prerequisite of device by 4 bulk absorptions of the absorption liquid in the scintillation vial 3.
Further; About kind and concentration as for injection; Principle is following: at first, the volume of acid is difficult for excessive: because whole device is airtight, thus extra outside system, add the pressure that liquid will change whole system, when system pressure is excessive; Might cause the destruction of stopping property, even small amount of liquid occur by the outer situation of pressure eliminating bottle.So we need select strong acid for use, and be concentrated acid.And the strong acid that obtains easily that has that we can select has: sulfuric acid, hydrochloric acid and nitric acid.Again because three kinds of acid of same concentration, vitriolic H+ concentration maximum (all the other both 2 times) is so we select sulfuric acid.Secondly, concentration can not overrich: because acid-base neutralisation can send a large amount of heat, if inject culturing bottle with the vitriol oil, will certainly produce a large amount of heat and make culturing bottle that the danger of blast arranged.So we are≤10mol/L at operable sulfuric acid concentration.
In sum, preferred version is: 4～10mol/L sulfuric acid.As long as certain other acid and concentration act on this and quite also can.
Again further, the seal assembly that the present invention adopts includes turned welt latex plug 8, and described turned welt latex plug 8 hoops are in the bottleneck outer of Tissue Culture Flask 1, scintillation vial 3; Turned welt latex and bottleneck interface are wound with and seal film 7.Thus, are the outsides that are wrapped in the turned welt plug through sealing film 7, can effectively seal the slit that possibly exist between turned welt plug and the bottleneck, seal film 7 and be wrapped in the position that both are connected, further increase stopping property.And transmission assembly is a disposal vacuum blood sampling conduit 6, and the two ends of this disposal vacuum blood sampling conduit 6 are provided with threads a needle.
In conjunction with practical application of the present invention, breadboard most cells, especially tumour cell all belong to attached cell, and the present invention very is fit to the cultivation of attached cell, thereby have increased the scope of application of this device greatly.This device is easy and simple to handle, and the operating process of cell cultures is similar with ordinary cells culturing bottle 1.
Through above-mentioned character express and combine accompanying drawing to find out; After adopting the present invention; Each assembly all can composition moduleization assembling, need disposable use to the radioactivity experiment, can replace the assembly that each has used at any time; Not only effectively reduce cost but also reduced the refuse volume, satisfied the sterilization demand simultaneously.And overall volume of the present invention is little, practices thrift culture space, is fit to cultivate in batches, can satisfy experimental requirements in batches.Moreover volume of the present invention is less, utilizes the present invention can realize the whole process that radioactive gas is collected, measured from producing to.
Claims (5)
1. a radioactive gas collecting cell culture apparatus includes Tissue Culture Flask, it is characterized in that: be injected with the radioactivity substratum in the said Tissue Culture Flask; Described Tissue Culture Flask bottleneck is provided with seal assembly; Be connected with the injection device that is used to stop the cellular metabolism reaction on the described Tissue Culture Flask; Described Tissue Culture Flask is connected into scintillation vial through transmission assembly; Described scintillation vial bottleneck is provided with seal assembly, is provided with absorption liquid in the described scintillation vial.
2. a kind of radioactive gas collecting cell culture apparatus according to claim 1, it is characterized in that: described radioactivity substratum contains
14C-glucose.
3. a kind of radioactive gas collecting cell culture apparatus according to claim 1, it is characterized in that: described injection device is an injector for medical purpose, injected material is H
2SO
4
4. a kind of radioactive gas collecting cell culture apparatus according to claim 1 is characterized in that: described transmission assembly is a disposal vacuum blood sampling conduit, and described disposal vacuum blood sampling conduit two ends are provided with threads a needle.
5. a kind of radioactive gas collecting cell culture apparatus according to claim 1, it is characterized in that: described seal assembly includes turned welt latex plug, and described turned welt latex plug hoop is in the bottleneck outer of Tissue Culture Flask, scintillation vial; Turned welt latex and bottleneck interface are wound with and seal film.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105306636A CN102465095A (en) | 2010-11-04 | 2010-11-04 | Radioactive gas collection cell culture apparatus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105306636A CN102465095A (en) | 2010-11-04 | 2010-11-04 | Radioactive gas collection cell culture apparatus |
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| CN102465095A true CN102465095A (en) | 2012-05-23 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102836442A (en) * | 2012-09-14 | 2012-12-26 | 苏州大学 | Application of DCX-SPRAC double-gene co-expression vector in preparation of radiotherapy sensitization agent |
| CN113498776A (en) * | 2021-06-17 | 2021-10-15 | 深圳逗点生物技术有限公司 | Biological transport system, biological transport method, and blood cell preservation system |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2424783Y (en) * | 2000-05-17 | 2001-03-28 | 张明德 | Radioactive carbon dioxide-absorbing device |
| CN1584558A (en) * | 2004-05-26 | 2005-02-23 | 中国科学院亚热带农业生态研究所 | Method and apparatus for measuring radioactive strength of plant and soil |
| CN201003056Y (en) * | 2007-02-12 | 2008-01-09 | 袁建华 | Cell culture bottle |
| CN101851580A (en) * | 2010-05-27 | 2010-10-06 | 苏州大学 | Cell culture chamber for collecting radioactive gases |
-
2010
- 2010-11-04 CN CN2010105306636A patent/CN102465095A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2424783Y (en) * | 2000-05-17 | 2001-03-28 | 张明德 | Radioactive carbon dioxide-absorbing device |
| CN1584558A (en) * | 2004-05-26 | 2005-02-23 | 中国科学院亚热带农业生态研究所 | Method and apparatus for measuring radioactive strength of plant and soil |
| CN201003056Y (en) * | 2007-02-12 | 2008-01-09 | 袁建华 | Cell culture bottle |
| CN101851580A (en) * | 2010-05-27 | 2010-10-06 | 苏州大学 | Cell culture chamber for collecting radioactive gases |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102836442A (en) * | 2012-09-14 | 2012-12-26 | 苏州大学 | Application of DCX-SPRAC double-gene co-expression vector in preparation of radiotherapy sensitization agent |
| CN113498776A (en) * | 2021-06-17 | 2021-10-15 | 深圳逗点生物技术有限公司 | Biological transport system, biological transport method, and blood cell preservation system |
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Application publication date: 20120523 |