CN202081110U - Constant concentration cell culture device with micropump control function - Google Patents
Constant concentration cell culture device with micropump control function Download PDFInfo
- Publication number
- CN202081110U CN202081110U CN 201120176710 CN201120176710U CN202081110U CN 202081110 U CN202081110 U CN 202081110U CN 201120176710 CN201120176710 CN 201120176710 CN 201120176710 U CN201120176710 U CN 201120176710U CN 202081110 U CN202081110 U CN 202081110U
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- CN
- China
- Prior art keywords
- syringe
- micropump
- bottle
- liquid outlet
- cell culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
Abstract
The utility model relates to a constant concentration cell culture device with a micropump control function, which comprises a micropump, a first syringe, a second syringe, sterile guide tubes, a clip, a culture bottle, a liquid inlet, an air vent, a liquid outlet, a bottle base and a liquid level. The first syringe and the second syringe are respectively fixed at the left end and the right end of the lower side of the micropump, push handles of an end a and an end b of the first syringe and the second syringe are fixed relatively and are fixedly connected with the same moving device below the micropump, the liquid inlet and the liquid outlet are respectively located on one side of a cover of the culture bottle and the lower portion of the right end of the culture bottle, the air vent is located on the upper side of the cover of the culture bottle, an output port of the first syringe is communicated with the liquid inlet via one sterile guide tube, and the second syringe is successively communicated with the clip and the liquid outlet via the other sterile guide tube. The constant concentration cell culture device with the micropump control function can provide and maintain a specific concentration environment for cell or tissue culture, and can be used for more accurately researching influence of an external environment with specific concentration to cells or tissues.
Description
Technical field
The utility model belongs to the biologic medical instrument field, particularly a kind of constant density cell culture apparatus with Micropump control.
Background technology
The active somatic cell vitro culture is an important process of biomedicine experiment and field of biological pharmacy.And explore new cell culture processes, researching and developing new cell culture apparatus is the important subject of biological and pharmaceutical industries with accuracy and the convenience that improves scientific experiment.
Existing cell culture processes is divided into two classes, i.e. culturing in vivo and vitro culture.Culturing in vivo be with cell inoculation in animal body, utilize the humoral system of animal itself to provide nutrition to target cell.Its advantage is that cell cultivation process is simple to operate, and cost is lower, but because of it has multiple complicated factor influence in vivo in culturing process, is difficult to the influence that clear and definite certain or certain several factor pair cell are cultivated, so this method has bigger limitation in scientific research.
The cells in vitro cultural method is to utilize Tissue Culture Dish, plate and the bottle made with glass or macromolecular material as container, and cell inoculation is cultivated in the said vesse that is marked with nutrient solution.In scientific research, often need the influence of certain predetermined substance of research processes such as cell growth, division, propagation under specific concentrations, the method of often taking to change at regular time and quantity the nutrient solution that contains the specific concentrations material in the former experiments is reached the effect of specific concentrations, process is more loaded down with trivial details, the input that needs great amount of manpower and material resources, also cause simultaneously the pollution of cell cultures easily, and the actual concentration that records often is lower than aimed concn in the process of cell cultures, easily causes experimental error.
The utility model can be cultivated for cell or tissue and provide and keep the particular concentration environment, studies the external environment pair cell of specific concentrations or the influence of tissue more accurately.
The utility model content
Technical problem to be solved in the utility model provides a kind of constant density cell culture apparatus with Micropump control, can cultivate for cell or tissue and provide and keep the particular concentration environment, study the external environment pair cell of specific concentrations or the influence of tissue more accurately.
The technical scheme that its technical problem that solves the utility model adopts is: a kind of constant density cell culture apparatus with Micropump control is provided, comprise Micropump, first syringe, second syringe, sterile catheter, clip, culturing bottle, fluid inlet, breather hole, liquid outlet, the bottle end and fluid level, two ends, the left and right sides, described Micropump below are fixed first syringe and second syringe respectively, and a, the b end of first syringe and second syringe pushes away the handle relative fixed and fixedlys connected with the same running gear of Micropump below; Fluid inlet and liquid outlet lay respectively at the two ends of culturing bottle bottle cap, and breather hole is positioned at culturing bottle bottle cap top; The delivery port of first syringe is communicated with fluid inlet by sterile catheter, and second syringe is communicated with clip and liquid outlet successively by sterile catheter.
Described breather hole is 4.
Beneficial effect
The utility model can be cultivated for cell or tissue and provide and keep the particular concentration environment, studies the external environment pair cell of specific concentrations or the influence of tissue more accurately.
Description of drawings
Fig. 1 is the utility model general assembly synoptic diagram.
Fig. 2 is the utility model culturing bottle synoptic diagram.
Fig. 3 looks synoptic diagram for the utility model culturing bottle bottle cap master.
Embodiment
Below in conjunction with specific embodiment, further set forth the utility model.Should be understood that these embodiment only to be used to the utility model is described and be not used in the restriction scope of the present utility model.Should be understood that in addition those skilled in the art can make various changes or modifications the utility model after the content of having read the utility model instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
As Fig. 1～shown in Figure 3, a kind of constant density cell culture apparatus with Micropump control comprises Micropump 1, first syringe, 2, the second syringes 3, sterile catheter 4, clip 5, culturing bottle 6, fluid inlet 6.1, breather hole 6.2, liquid outlet 6.3, a, the b end that the bottle end 6.4 and fluid level 6.5, two ends, the left and right sides, described Micropump 1 below are fixed first syringe 2 and second syringe, 3, the first syringes 2 and second syringe 3 respectively pushes away the handle relative fixed and fixedlys connected with the same running gear c of Micropump 1 below; Fluid inlet 6.1 and liquid outlet 6.3 lay respectively at the two ends of culturing bottle 6, and breather hole 6.2 is positioned at the top of culturing bottle 6 bottle caps; The delivery port of first syringe 2 is communicated with fluid inlet 6.1, the second syringes 3 by sterile catheter 4 and is communicated with clip 5 and liquid outlet 6.3 successively by sterile catheter 4.
Described breather hole 6.2 is 4.
During use, at first before experiment, adjust the height of fluid levels 6.5 by clip 5 closures, open clip 5 after reaching the expection fluid level, start Micropump 1 (speed of Micropump is pressed the actual needs adjusting in experiment), the nutrient solution that Micropump drive running gear b moves constant densities in first syringe 2 is squeezed into culturing bottle 6, original nutrient solution is then by extracting out with second syringe 3 of first syringe, 2 interlocks in the culturing bottle 6, so far, syringe 2, culturing bottle 6, syringe 3 and sterile catheter 4 form the culture system of a closed uniflux, can satisfy the needs of culturing cell under the specific concentrations.
Claims (2)
1. constant density cell culture apparatus with Micropump control, comprise Micropump (1), first syringe (2), second syringe (3), sterile catheter (4), clip (5), culturing bottle (6), fluid inlet (6.1), breather hole (6.2), liquid outlet (6.3), the bottle end (6.4) and fluid level (6.5), it is characterized in that: two ends, the left and right sides, described Micropump (1) below are fixed first syringe (2) and second syringe (3) respectively, a of first syringe (2) and second syringe (3), the b end pushes away the handle relative fixed and fixedlys connected with the same running gear (c) of Micropump (1) below; Fluid inlet (6.1) and liquid outlet (6.3) lay respectively at the two ends of culturing bottle (6) bottle cap, and breather hole (6.2) is positioned at culturing bottle (6) bottle cap top; The delivery port of first syringe (2) is communicated with fluid inlet (6.1) by sterile catheter (4), and second syringe (3) is communicated with clip (5) and liquid outlet (6.3) successively by sterile catheter (4).
2. a kind of constant density cell culture apparatus with Micropump control according to claim 1, it is characterized in that: described breather hole (6.2) is 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120176710 CN202081110U (en) | 2011-05-27 | 2011-05-27 | Constant concentration cell culture device with micropump control function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120176710 CN202081110U (en) | 2011-05-27 | 2011-05-27 | Constant concentration cell culture device with micropump control function |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202081110U true CN202081110U (en) | 2011-12-21 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120176710 Expired - Fee Related CN202081110U (en) | 2011-05-27 | 2011-05-27 | Constant concentration cell culture device with micropump control function |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202081110U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106148178A (en) * | 2015-04-02 | 2016-11-23 | 复旦大学附属华山医院 | The culture systems of a kind of static killing curve for anti-anaerobic agent and construction method thereof |
-
2011
- 2011-05-27 CN CN 201120176710 patent/CN202081110U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106148178A (en) * | 2015-04-02 | 2016-11-23 | 复旦大学附属华山医院 | The culture systems of a kind of static killing curve for anti-anaerobic agent and construction method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111221 Termination date: 20140527 |