CN202347025U - Automatic cell culture device capable of simulating internal environments of organism - Google Patents
Automatic cell culture device capable of simulating internal environments of organism Download PDFInfo
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Abstract
The utility model belongs to the technical field of cell culture, particularly discloses an automatic cell culture device capable of simulating internal environments of organism, and comprises a laminar flow sterile sealed chamber. An internal environment simulation tissue and organ culture device and a target cell induction culture device are arranged in the sealed chamber. The automatic cell culture device can simulate the internal environments including a low-oxygen low-permeability environment for culturing embryonic stem cells, a testicular internal environment during maturation of germ cells, a thymus environment for inducing maturation of T cells, a cerebrospinal fluid environment for inducing differentiation of neural stem cells and tissue and organ environments such as livers, pancreas, bone marrow and tumors for inducing differentiation and maturation of corresponding stem cells, and cultures specific high-quality cells in an induction mode to meet needs of cell basic researches and application. Besides, the simulated internal environments provide a basis for researching an organism cell growth, differentiation and maturation mechanism. Therefore, the improvement of cell culture quality is facilitated, repeatability of cytology researches is remarkably increased, cost of cell culture is saved, clinic transformation and application of the cell culture technology are quickened, and a good experiment platform is provided for scientific research basic workers.
Description
Technical field
The utility model belongs to the cell culture technology field.The concrete cell robotization culture apparatus that discloses a kind of analog machine internal milieu.
Background technology
Cell cultures is the basis of biotechnology, bio-pharmaceuticals, the utilization of Biological resources sustainability and cell medical treatment, and perfect cell culture technology system is the key that guarantees scientific research, production and medical use.The intravital cell of machine residing environment in growth and atomization is different, and its biological characteristics also can change with environment.If will go out the cell of true reflection body characteristic in vitro culture, just suitable coenocorrelation need be arranged.Like environment in the testis in the hypotonic environment of the hypoxemia of embryonic stem cell, the sexual cell maturation process during fetal growth, inducing T cell fully-developed thymus gland environment, the interior environment of marrow of keeping the bone marrow stem cell proliferation potential and induced nerve stem cells fully-developed cerebrospinal fluid environment etc.And existing cell culture system is not only aspect the analog machine internal milieu, and robotization cultivate with real-time monitoring aspect still unsatisfactory.
Particularly in medicine technology field, U.S. FDA and ministry of Health of China have all been ratified the immunocyte treatment of cancer and have been treated with hemopoietic stem cell.Cell therapy is to gather the patient has therapeutic action in body or allosome blood, marrow and various organs and tissues cell (like immune lymphocyte and stem cell etc.); Through extracorporeal treatment such as inducing culture and amplification cultivation, be fed back to the intravital a kind of novel method of treatment of patient after making the effector cell reach the clinical treatment standard.Immunocyte treatment and hemopoietic stem cell treatment cancer obtain domestic and international medical institutions and use increasingly extensively, have and treat effect preferably.Other stem-cell therapy research is also goed deep into gradually, and stem cells technology is widely used in the treatment of difficult and complicated illness such as Spinal injury, autism, mongolism, brain paralysis, cerebral trauma, progressive muscular dystrophy, ataxia, motor neuron, cerebral dysplasia, encephalitis sequela, diabetic foot.Yet; Because existing cell culture system is still unsatisfactory aspect analog machine internal milieu, robotization cultivation and monitoring in real time; Vitro culture stem cells technology still difficulty reaches the clinical application standard; Stem cell all directly is collected in the patient or the allosome that matches basically, and stem-cell therapy usually faces and lacks the deficiencies such as donor and stem cell collection quantity that match.And the vitro culture process of existing immunocyte treatment is generally accomplished by manual operation, and the cultivation operating process is loaded down with trivial details, is prone to microbial contamination and crossed contamination take place, and is difficult to guarantee the asepticize production of whole process; The artificial culture method, repeatable low, quality is unstable; Also have the effector cell to induce simultaneously and the quantity that increases few, it is higher to cultivate cost, the lymphocyte that need repeatedly gather the patient could satisfy the needs of cancer repeated multiple times treatment.
In order to address the above problem; The applicant has applied for the patent that an application number is 201020635650.0, the utility model name is called medical cell automatic production device on December 1st, 2010, and this patent can realize robotization fully, and operating process is simple and convenient; Operate miss is little; Good reproducibility meets the GMP standard, can be adaptable across different operating environment and occasions such as medical treatment and bio-pharmaceuticals.The Growth and Differentiation of cell depends on specific growing environment of body and intercellular interaction; Like environment in the testis in the hypotonic environment of the hypoxemia of embryonic stem cell, the sexual cell maturation process during fetal growth; Inducing T cell fully-developed thymus gland environment, multipotential stem cell are divided into the myelencephalon environment of NSC and neurocyte etc.; Yet the design of existing cell culture apparatus analog machine internal milieu shortcoming is difficult to satisfy the cultivation requirement of specific cells.
The utility model content
The purpose of the utility model is the deficiency that overcomes prior art; A kind of automatic cytological culture apparatus of analog machine internal milieu is provided; This device can be simulated the interior environment of the hypotonic environment of hypoxemia, the testis in the sexual cell maturation process during fetal growth of embryonic stem cell, and inducing T cell fully-developed thymus gland environment, multipotential stem cell are divided into the myelencephalon environment of NSC and neurocyte, and development of stem cells becomes the required microenvironment of histoorgans such as liver, pancreas, marrow; Inducing culture goes out the needs that specific high quality cell satisfies cell base research and uses; The mimic internal milieu is also laid a good foundation for research aircraft somatocyte Growth and Differentiation mechanism simultaneously, helps improving the cell cultures quality, increases the repeatability of cell experiment; Practice thrift the cell cultures cost; Accelerate the clinical conversion and the application of cell culture technology, the application that has improved cell on the one hand benefits the society, and also for scientific research element task person good experiment porch is provided on the other hand.
In order to realize above-mentioned technical purpose, the utility model is realized by following technical scheme:
The automatic cytological culture apparatus of the described a kind of analog machine internal milieu of the utility model; Comprise the sealing chamber that laminar flow is aseptic; Environmental simulation histoorgan culture apparatus and purpose cell induction culture apparatus in being provided with in the said sealing chamber; Carry out the tissue or the cell cultures of organs such as thymus gland, testis, tumour and marrow; With the interior environment of simulation respective organization organ, be provided with simulated tissue organ sample inlet and purpose cell culture sample inlet in the said sealing chamber, said histoorgan sample inlet is connected with the interior environmental simulation histoorgan culture apparatus that the simulated tissue organ is cultivated; Said purpose cell culture sample inlet connects purpose cell induction cultured cells inducing culture device; Through pipe connecting successively connect the purpose cell is carried out reaction unit between organismic internal environment simulation stimulated cells thereafter; Said iuntercellular reaction unit comprises the iuntercellular retort and places malleation fluid exchange pipe, fluid exchange capillary bed and the negative pressure liquid exchange pipe that is interconnected in the iuntercellular retort; Said purpose cell induction culture apparatus inlet is communicated with negative pressure liquid exchange pipe through pipe connecting; The outlet of said purpose cell induction culture apparatus be communicated with malleation fluid exchange pipe through pipe connecting; The connecting pipeline of said malleation fluid exchange pipe is provided with positive pressure devices, and the connecting pipeline of negative pressure liquid exchange pipe is provided with negative pressure device.
Further improvement as above-mentioned technology; Environmental simulation histoorgan culture apparatus comprises interior environmental chamber in said; Said iuntercellular retort places interior environmental simulation indoor, guarantees that like this malleation fluid exchange pipe, fluid exchange capillary bed and negative pressure liquid exchange pipe extensively contact with the interior nutrient solution of interior environmental simulation histoorgan culture apparatus, can reach the unidirectional and two-way exchange of liquid; To stimulate purpose cytodifferentiation and propagation, inducing culture goes out specific cell.
Further improvement as above-mentioned technology; Be equipped with nutrient solution come in and go out pipeline put in place reminder sensor or TP or pH value transmitter or pressure transmitter or dissolved oxygen sensor in interior environmental simulation histoorgan culture apparatus and the purpose cell induction culture apparatus; Show the culture parameters in each culture apparatus in real time; Each transmitter is connecting unit and computer supervisory control system, operable automatically control culture condition.
Further improvement as above-mentioned technology; Pipeline between the positive and negative pressure fluid exchange pipe of said purpose cell induction culture apparatus and iuntercellular reaction unit is provided with pressure-controlling by-pass cock and pressure inductor; The pressure difference of monitoring iuntercellular reaction unit is regulated the fluid exchange between pipeline liquid flow rate and culture apparatus in real time.
The principle of work of environment cell robotization culture apparatus in the described simulation of the utility model below is described:
Purpose cell in the purpose cell induction culture apparatus and nutrient solution thereof flow into malleation fluid exchange pipe, fluid exchange kapillary and negative pressure liquid exchange pipe through pipe connecting successively under positive pressure devices drives; Medical cell in the negative pressure liquid exchange pipe and nutrient solution thereof are back to the cell induction culture apparatus under the attraction of negative pressure device.Wherein a part of purpose cell and nutrient solution thereof flow into the cell refining plant, to reduce the meta-bolites and interpolation fresh medium in the nutrient solution.Purpose cell and nutrient solution thereof in malleation fluid exchange pipe, fluid exchange capillary bed and three parts of negative pressure liquid exchange pipe with interior environmental simulation histoorgan culture apparatus in the EF and the nutrient solution thereof of simulation histoorgan carry out fluid exchange; These EFs such as Zadaxin etc. stimulate purpose cytodifferentiation propagation, and inducing culture goes out specific cell.
The purpose cell and the nutrient solution thereof that order about in the purpose cell induction culture apparatus in the positive pressure devices described in the utility model slowly flow into the iuntercellular reaction unit, and impel the nutrient solution of purpose cell to enter to interior environmental simulation histoorgan culture apparatus; Negative pressure device control negative pressure liquid exchanges the pressure-reduction level of pipe, thereby helps sucking the EF and the nutrient solution of the simulated tissue organ in the interior environment simulator.Fluid exchange capillary bed (or fluid exchange jar) then increases medical cell and simulated tissue organ cell's contact area and time, thereby realizes the cell-cell interaction of high efficiency purpose cell and simulated tissue organ.This malleation exchange pipe, capillary bed and negative pressure exchange pipe are human body artery, capillary vessel and venous biomimetic features; The simulated environment of histoorgan is provided for the growth of purpose cell; Help purpose cytodifferentiation, propagation and maturation, improve purpose cell cultures quality.
Compared with prior art, the beneficial effect of the utility model is:
(1) the automatic cytological culture apparatus of the said analog machine internal milieu of the utility model can be simulated the interior environment of the hypotonic environment of hypoxemia, the testis in the sexual cell maturation process during fetal growth of embryonic stem cell; Inducing T cell fully-developed thymus gland environment, multipotential stem cell are divided into the myelencephalon environment of NSC and neurocyte; And development of stem cells becomes the required microenvironment of histoorgans such as liver, pancreas, marrow; Help purpose cytodifferentiation, propagation and maturation, improve the cell cultures quality.
(2) the said analog machine internal milieu of the utility model the automatic cytological culture apparatus can be in cell cultivation process; The monitoring of realization whole-course automation; Significantly increase the repeatability of cell experiment; Help exploring cells in vitro propagation, differentiation and ripe rule, help screening the best cultural method of cell, help the basic and applied research of cell medical treatment.
(3) simulated in vivo environment the successful development of automatic cytological culture apparatus can realize automatic production; Be convenient to the GMP management; Help improving the cell cultures quality, practice thrift the cell cultures cost, accelerate the conversion and the application of cell culture technology; To improving the people ' s health level, promote China's biological medicine competitive power and have great pushing effect.
(4) the described simulated in vivo environment of the utility model the automatic cytological culture apparatus can simulate environment in the hypotonic environment of hypoxemia, the testis in the sexual cell maturation process during fetal growth of embryonic stem cell; Inducing T cell fully-developed thymus gland environment, multipotential stem cell are divided into the myelencephalon environment of NSC and neurocyte; And development of stem cells becomes the required microenvironment of histoorgans such as liver, pancreas, marrow; Be convenient to inducing culture and go out fundamental research and the application need that specific cell satisfies the cell medical treatment; The mimic internal milieu is also laid a good foundation for research aircraft somatocyte function and Growth and Differentiation mechanism simultaneously; Benefit the society so improved the application of cell on the one hand, also good experiment porch is provided on the other hand for scientific research element task person.
Description of drawings
Below in conjunction with accompanying drawing and specific embodiment the utility model is done detailed explanation:
Fig. 1 is the automatic cytological culture apparatus structural representation of the utility model embodiment one described analog machine internal milieu.
Fig. 2 is the automatic cytological culture apparatus structural representation of the utility model embodiment two described analog machine internal milieus.
Embodiment
As shown in Figure 1; The automatic cytological culture apparatus of the described a kind of analog machine internal milieu of the utility model; Comprise the aseptic sealing chamber of laminar flow 1; Be provided with interior environmental simulation histoorgan culture apparatus 2 and purpose cell induction culture apparatus 3 in the said sealing chamber 1, carry out the tissue or the cell cultures of organs such as thymus gland, testis, tumour and marrow, with the interior environment of simulation respective organization organ; Be provided with simulated tissue organ sample inlet I and purpose cell culture sample inlet II in the said sealing chamber 1, said histoorgan sample inlet I is connected with the interior environmental simulation histoorgan culture apparatus 2 that the simulated tissue organ is cultivated; Said purpose cell culture sample inlet II with purpose cell induction cultured cells inducing culture device 3 is connected; Through pipe connecting successively connect the purpose cell is carried out reaction unit 4 between organismic internal environment simulation stimulated cells thereafter; The reaction of said iuntercellular is adorned 4 and is put and comprise iuntercellular retort 41 and place malleation fluid exchange pipe 42, fluid exchange capillary bed 43 and the negative pressure liquid exchange pipe 44 that is interconnected in the iuntercellular retort 41; Said purpose cell induction culture apparatus 3 inlets are communicated with negative pressure liquid exchange pipe 44 through pipe connecting; The outlet of said purpose cell induction culture apparatus 3 be communicated with malleation fluid exchange pipe 42 through pipe connecting; The connecting pipeline of said malleation fluid exchange pipe 42 is provided with positive pressure devices 5, and the connecting pipeline of negative pressure liquid exchange pipe 44 is provided with negative pressure device 6.In addition, for the size of real-time regulated and control pressure, the pipeline between the negative pressure liquid of said purpose cell induction culture apparatus 3 and the interior environmental simulation histoorgan culture apparatus 2 exchange pipe 43 also is provided with negative pressure regulating and controlling switch 7 and negative pressure transducer 8; Pipeline between the malleation fluid exchange pipe 22 of said purpose cell induction culture apparatus 3 and interior environmental simulation histoorgan culture apparatus 2 also is provided with malleation regulating and controlling switch 9 and positive pressure sensor 10.
Be equipped with nutrient solution come in and go out pipeline put in place reminder sensor or TP or pH value transmitter or pressure transmitter or dissolved oxygen sensor in interior environmental simulation histoorgan culture apparatus 2 and the purpose cell induction culture apparatus 3; Show the culture parameters in each culture apparatus in real time; Each transmitter is connecting unit and computer supervisory control system, operable automatically control culture condition.
Pressure-controlling by-pass cock and pressure transmitter that pipeline between the malleation fluid exchange pipe 42 of said purpose cell induction culture apparatus 3 and iuntercellular reaction unit 4, the negative pressure liquid exchange pipe 43 is provided with; The pressure difference of monitoring iuntercellular reaction unit is regulated the fluid exchange between pipeline liquid flow rate and culture apparatus in real time.
The principle of work of environment cell robotization culture apparatus in the said simulation of the utility model below is described:
During work, medical cell and culture thereof that positive pressure devices 5 is ordered about in the purpose cell induction culture apparatus 3 slowly flow into iuntercellular retort 41, and impel the nutrient solution of purpose cell to enter interior environmental simulation jar 21; The pressure-reduction level of negative pressure device 6 control negative pressure liquid exchange pipes, thereby the nutrient solution in the environmental simulation jar 21 in helping sucking.Fluid exchange capillary bed 43 (or fluid exchange jar) then increases purpose cell and simulated tissue organ cell's contact area and time, thereby realizes the cell-cell interaction of high efficiency medical cell and simulated tissue organ.This malleation exchange pipe, capillary bed and negative pressure exchange pipe are human body artery, capillary vessel and venous biomimetic features; The simulated environment of histoorgan is provided for the growth of medical cell; Help medical cytodifferentiation, propagation and maturation, improve purpose cell cultures quality.
The medical cell robotization culture apparatus of the described analog machine internal milieu of the utility model can be simulated the interior environment of the hypotonic environment of hypoxemia, the testis in the sexual cell maturation process during fetal growth of embryonic stem cell; Inducing T cell fully-developed thymus gland environment, multipotential stem cell are divided into the myelencephalon environment of NSC and neurocyte; And development of stem cells becomes the required microenvironment of histoorgans such as liver, pancreas, marrow; Be convenient to inducing culture and go out fundamental research and the application need that specific cell satisfies the cell medical treatment; The mimic internal milieu is also laid a good foundation for research aircraft somatocyte function and Growth and Differentiation mechanism simultaneously; Benefit the society so improved the application of cell on the one hand, also good experiment porch is provided on the other hand for scientific research element task person; The automatic cytological culture apparatus of simulated in vivo environment helps improving the cell cultures quality; Practice thrift the cell cultures cost; Accelerate the conversion and the application of cell culture technology,, promote China's biological medicine competitive power and have great pushing effect improving the people ' s health level; Repeatability with increasing cell culture experiments helps exploring cells in vitro propagation, differentiation and ripe rule, helps screening the best cultural method of cell, helps the clinical study of cell medical treatment.
Embodiment two:
As shown in Figure 2; Present embodiment and embodiment one are basic identical; Its difference is: environment simulator is two in said; Comprise first interior environmental simulation histoorgan culture apparatus 2a that is used to simulate environment in thymus gland, the lymphoglandula and the interior environmental simulation histoorgan culture apparatus 2b that is used to simulate environment in the tumour; The effector cell that can according to actual needs medical cell induction culture apparatus 1 be obtained is delivered among the first interior environmental simulation histoorgan culture apparatus 2a or the second interior environmental simulation histoorgan culture apparatus 2b accordingly; Exchange the cooperation simulation organismic internal environment of the pressure reduction of pipe 44 through malleation fluid exchange pipe 42, negative pressure liquid, and prolong the time that effector cells retain in the environment in this mimic, effectively improve effector cell's quality through fluid exchange capillary bed 43.
Claims (4)
1. the automatic cytological culture apparatus of an analog machine internal milieu; Comprise the sealing chamber that laminar flow is aseptic; It is characterized in that: environmental simulation histoorgan culture apparatus and purpose cell induction culture apparatus in being provided with in the said sealing chamber; Be provided with simulated tissue organ sample inlet and purpose cell culture sample inlet in the said sealing chamber, said histoorgan sample inlet connects the interior environmental simulation histoorgan culture apparatus that the simulated tissue organ is cultivated; Said purpose cell culture sample inlet connects purpose cell induction cultured cells inducing culture device; Through pipe connecting successively connect the purpose cell is carried out reaction unit between organismic internal environment simulation stimulated cells thereafter; Said iuntercellular reaction unit comprises the iuntercellular retort and places malleation fluid exchange pipe, fluid exchange capillary bed and the negative pressure liquid exchange pipe that is interconnected in the iuntercellular retort; Said purpose cell induction culture apparatus inlet is communicated with negative pressure liquid exchange pipe through pipe connecting; The outlet of said purpose cell induction culture apparatus be communicated with malleation fluid exchange pipe through pipe connecting; The connecting pipeline of said malleation fluid exchange pipe is provided with positive pressure devices, and the connecting pipeline of negative pressure liquid exchange pipe is provided with negative pressure device.
2. the automatic cytological culture apparatus of analog machine internal milieu according to claim 1 is characterized in that: environmental simulation histoorgan culture apparatus comprises interior environmental chamber in said, and environmental simulation was indoor in said iuntercellular retort placed.
3. the automatic cytological culture apparatus of analog machine internal milieu according to claim 2; It is characterized in that: be equipped with nutrient solution discrepancy pipeline put in place reminder sensor or TP or pH value transmitter or pressure transmitter or dissolved oxygen sensor in said in environmental simulation histoorgan culture apparatus and the purpose cell induction culture apparatus, be connected with unit and computer supervisory control system behind each transmitter.
4. the automatic cytological culture apparatus of analog machine internal milieu according to claim 3 is characterized in that: the pipeline between the positive and negative pressure fluid exchange pipe of said purpose cell induction culture apparatus and iuntercellular reaction unit is provided with pressure-controlling by-pass cock and pressure inductor.
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| CN 201120521341 CN202347025U (en) | 2011-12-13 | 2011-12-13 | Automatic cell culture device capable of simulating internal environments of organism |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103160434A (en) * | 2011-12-13 | 2013-06-19 | 汪华 | An automatic cell culture device capable of simulating internal environments of organisms |
| CN109153955A (en) * | 2016-06-08 | 2019-01-04 | X开发有限责任公司 | In silico simulation of living cells |
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2011
- 2011-12-13 CN CN 201120521341 patent/CN202347025U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103160434A (en) * | 2011-12-13 | 2013-06-19 | 汪华 | An automatic cell culture device capable of simulating internal environments of organisms |
| CN103160434B (en) * | 2011-12-13 | 2014-06-18 | 汪华 | An automatic cell culture device capable of simulating internal environments of organisms |
| CN109153955A (en) * | 2016-06-08 | 2019-01-04 | X开发有限责任公司 | In silico simulation of living cells |
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