CN201587946U - In vitro co-culture apparatus for multiple types of cells - Google Patents
In vitro co-culture apparatus for multiple types of cells Download PDFInfo
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- CN201587946U CN201587946U CN2010200101813U CN201020010181U CN201587946U CN 201587946 U CN201587946 U CN 201587946U CN 2010200101813 U CN2010200101813 U CN 2010200101813U CN 201020010181 U CN201020010181 U CN 201020010181U CN 201587946 U CN201587946 U CN 201587946U
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Abstract
An in vitro co-culture apparatus for multiple types of cells comprises a culture dish, wherein a microporous membrane of which the pore diameter is smaller than the diameter of the culture cell is arranged in the culture dish; and the microporous membrane partitions the culture dish into a plurality of culture rooms. When the apparatus is used for cell culture, different types of cells are respectively inoculated into different culture rooms, an in vitro cell growth model which is closer to the in vivo tissues is built and used for simulation test before the drug clinical trial, and the studying of the possible influence of the drug on the cells in vivo and the understanding of the physiological functions of the tissues and organs in vitro are possible.
Description
One, technical field
The utility model relates to the utensil that medically is used for cell cultures, is specially one kind of multiple cells in vitro associating culture apparatuses.
Two, background technology
Cell culture technology is the common technology of preclinical medicine, for example develops a kind of new drug for the treatment of tumour, normally does the research of cell level earlier, carries out experimentation on animals then, carries out clinical observation at last.Cell cultures is the basis of a series of activities.
Higher organism is the integral body that is made of many cells, will study individual cells or certain a group cell functional activity in vivo is very difficult under whole condition.Observe and study so will take vitro culture to viable cell, so the cell culture technology that just has been born.To under certain physiological condition, could survive after viable cell exsomatizes and carry out physiological activity, have the discomfort will death slightly.
Be divided into attached cell (when cell leaves standstill and cultivates according to the characteristics institute cultured cells of cultured cells in nutrient solution in incubator, the heavy bottom that invests incubator of cell) and suspension cell (cell is under normal circumstances not adherent, as lymphocyte, be the suspension growth).
Existing cell culture technology only designs for the single culture of attached cell or suspension cell, and its device is culture dish or culturing bottle.But the higher organism organism is the integral body that is made of many cells, break away from whole and cell that only observe single kind can not be understood its function in organism fully, and directly use organism research, comprise that experimentation on animals and clinical observation all relate to problems such as Medical Ethics, laws and regulations, policy access again.Therefore, in the cell in vitro culturing process, make up a many cells mutual survival, and can analogue body in the device of living environment be very necessary to external many cells research.
Three, utility model content
The purpose of this utility model provides one kind of multiple cells in vitro associating culture apparatuses for satisfying above-mentioned needs exactly.
The external associating culture apparatus of the various kinds of cell that the utility model provides comprises culture dish, is equipped with micro-pore septum in culture dish, and micro-pore septum is separated into a plurality of culturing room with culture dish, and the aperture of micro-pore septum is less than the diameter of institute's culturing cell.The number of plies of micro-pore septum is decided according to the quantity of institute's culturing cell kind.
The cell that carries out vitro culture with the utility model device should be the adjacent cell in position, for example tumour cell and a stroma cell thereof in organism.
When the utility model device is used for two or more cell cultures, in culture dish, add cell culture fluid earlier, then different cells is placed the different culturing room that are separated into by micro-pore septum.Because membranous aperture is less than the diameter of institute's culturing cell, making not, allogenic cell can not mix mutually; And micro-pore septum allows that fluid molecule and gas molecule freely pass through, so the enzyme of emiocytosis, cytokine, meta-bolites can pass through the micro-pore septum disperse
To other cells.Cell category is many more, more near real living environment in the body.During research, can successively take out micro-pore septum, put into other culture dish digestion, draw.
The utility model device is applied to a kind of attached cell that is, can be when another kind is two kinds of cell cultures of suspension cell without micro-pore septum, take off micro-pore septum this moment, with selected attached cell direct inoculation in Tissue Culture Dish, material as culture dish is a glass, needing to add certain density gelatin and be beneficial to attached cell and seek connections with before inoculation, is that plastics then need not add gelatin as the material of culture dish.After treating that the attached cell growth is stable, add suspension cell in the clear liquid thereon, make two kinds of cell symbiosis.Draw suspension cell during research earlier, add the trysinization attached cell again, make it become suspension, draw research.
The utility model device has following advantage:
1, utilizing this device, can be that simulation test is understood its may influencing of pair cell in vivo at the clinical drug trial previous crops.
2, because of tissue, organ is made of various kinds of cell, the in-vitro cell growth model of being set up with the utility model device more approaches the growing state of in-vivo tissue, makes the physiological function at external understanding tissue, organ become possibility.
3, this device using method is simple, and is easy to operate, and can use freeze-stored cell is finished testing apparatus in the short period of time structure, can both operate by the those of ordinary skill of cell cultivate professional training, is easy to promote.
Four, description of drawings
Accompanying drawing is an embodiment of the present utility model--be provided with the structure and the use schematic perspective view of the external associating culture apparatus of various kinds of cell of two-layer micro-pore septum.
Five, embodiment
In conjunction with the accompanying drawings, the culture dish 1 of the external associating culture apparatus of present embodiment various kinds of cell is circular, make by the colourless transparent resin material, internal diameter 30mm, high 10mm, micro-pore septum 9 and 10 usefulness skeletons (overlap with micro-pore septum among the figure, show) and the support bar 11,12 that is installed in skeleton top be lifted in the culture dish ( support bar 11 and 12 outer end frame are on the upper limb of culture dish), micro-pore septum is made by the polycarbonate material, aperture 5 μ m, its diameter is respectively 25mm and 20mm, highly is respectively 7mm and 5mm.Two-layer micro-pore septum becomes first culturing room 3, second culturing room 5 and the 3rd culturing room 7 with the separated by spaces in the culture dish.For avoiding polluting, can add a bacterium shield cap 13 in the culture dish outside.When being used for cell cultures, in culture dish, add cell culture fluid 2, then inoculation the first layer culturing cell 4, second layer culturing cell 8 and the 3rd layer of culturing cell 6 in first, second and the 3rd culturing room respectively as required.
Be example to utilize this certain medicine of device research to the influence of leukemia cell in the body now, organize in contrast that second culturing room with the leukemia cell implants this device gives drug intervention then, whether observation apoptosis occurs.As test group, get another this device, in its first culturing room, implant endotheliocyte, implant the leukemia cell in second culturing room, implant adipocyte in the 3rd culturing room, give then with the concentration medicine, whether the observation leukemia cell apoptosis.Contrast two groups of results, positive as control group, test group is negative, illustrates that then this medicine may not have killing action to the leukemia cell in vivo, and clinical use is had any problem; All positive as control group and test group, illustrate that this medicine has killing action to the leukemia cell in vivo, has the clinical expansion prospect.
Claims (1)
1. one kind of multiple cells in vitro are united culture apparatuses, comprise culture dish (1), it is characterized in that, in culture dish (1), be equipped with micro-pore septum (9,10), micro-pore septum is separated into a plurality of culturing room (3,5,7) with culture dish, and the aperture of micro-pore septum is less than the diameter of institute's culturing cell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010200101813U CN201587946U (en) | 2010-01-12 | 2010-01-12 | In vitro co-culture apparatus for multiple types of cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010200101813U CN201587946U (en) | 2010-01-12 | 2010-01-12 | In vitro co-culture apparatus for multiple types of cells |
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| Publication Number | Publication Date |
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| CN201587946U true CN201587946U (en) | 2010-09-22 |
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| CN2010200101813U Expired - Fee Related CN201587946U (en) | 2010-01-12 | 2010-01-12 | In vitro co-culture apparatus for multiple types of cells |
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| CN (1) | CN201587946U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105296350A (en) * | 2015-11-30 | 2016-02-03 | 苏州钧隆塑胶有限公司 | Bacterium cultivation bottle with multi-layer movable bottle bottoms |
| CN109554296A (en) * | 2018-12-19 | 2019-04-02 | 南方医科大学第三附属医院（广东省骨科研究院） | A kind of in-vitro simulated circulating tumor cell generation device and its application |
| CN110072988A (en) * | 2016-10-28 | 2019-07-30 | 国立大学法人京都大学 | The co-culture device and co-culture method of the bacteriums such as Anaerobic Bacteria and epithelial cell |
| CN114600835A (en) * | 2022-03-08 | 2022-06-10 | 郑羽 | Auxiliary system for establishing PDX model in laboratory and control method thereof |
-
2010
- 2010-01-12 CN CN2010200101813U patent/CN201587946U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105296350A (en) * | 2015-11-30 | 2016-02-03 | 苏州钧隆塑胶有限公司 | Bacterium cultivation bottle with multi-layer movable bottle bottoms |
| CN110072988A (en) * | 2016-10-28 | 2019-07-30 | 国立大学法人京都大学 | The co-culture device and co-culture method of the bacteriums such as Anaerobic Bacteria and epithelial cell |
| CN109554296A (en) * | 2018-12-19 | 2019-04-02 | 南方医科大学第三附属医院（广东省骨科研究院） | A kind of in-vitro simulated circulating tumor cell generation device and its application |
| CN109554296B (en) * | 2018-12-19 | 2024-03-26 | 南方医科大学第三附属医院（广东省骨科研究院） | In-vitro simulated circulation tumor cell generation device and application thereof |
| CN114600835A (en) * | 2022-03-08 | 2022-06-10 | 郑羽 | Auxiliary system for establishing PDX model in laboratory and control method thereof |
| CN114600835B (en) * | 2022-03-08 | 2023-01-24 | 郑羽 | Auxiliary system for establishing PDX model in laboratory and control method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100922 Termination date: 20120112 |