CN208748113U - A kind of more spatial cell culture apparatus - Google Patents
A kind of more spatial cell culture apparatus Download PDFInfo
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- CN208748113U CN208748113U CN201821156238.3U CN201821156238U CN208748113U CN 208748113 U CN208748113 U CN 208748113U CN 201821156238 U CN201821156238 U CN 201821156238U CN 208748113 U CN208748113 U CN 208748113U
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- culturing room
- bottle cap
- bottleneck
- cell culture
- culture apparatus
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Abstract
The utility model provides a kind of more spatial cell culture apparatus, including the first culturing room, several is set to indoor second culturing room of first culture；First culturing room is equipped with the first bottleneck and covers tightly the first bottle cap of the first bottleneck；Second culturing room is equipped with the second bottleneck and covers tightly the second bottle cap of the second bottleneck, and the second bottleneck is set to or stretches out the top of the first culturing room；The wall surface of second culturing room is semi-permeable membrane.The utility model can reasonably arrange each culturing room according to the demand of cell culture, and feeder cells are cultivated in the second culturing room to realize, and cultivate aim cell in the first culturing room.
Description
Technical field
The utility model relates to technical field of cell culture more particularly to a kind of more spatial cell culture apparatus.
Background technique
Cell culture refers to all in vitro cultures, and meaning, which refers to, takes out tissue in driven object living body body, in analogue body
Physiological environment etc. it is specific in vivo under the conditions of, carry out incubation culture, be allowed to survive and grow.Cell culture technology can be by one
Cell becomes many cells that are simple unicellular or seldom breaking up by mass propgation, this is the essential ring of clone technology
Section, and the clone of cell culture inherently cell.For entire biotechnology, cell culture be all one must
Indispensable process.Cell culture work has been widely used in the every field such as biology, medicine, new drug development, becomes most
One of important basic science.
Currently, there is many cells (such as embryonic stem cell), the primary or initial-stage culture stage, which generally all needs to depend on, to secrete
They survive in vitro is proliferated the feeder cells of necessary growth factor.The growth of different types of feeder cells secretion
The factor is slightly different, but is required that the feeder cells in incubation do not divide and be not proliferated and still keep metabolic activity.Growth
The factor is cell culture, especially the common growing multiplication promotor of embryonic stem cell culture and differentiation inhibitors.But for
For the cell of clinical application, the intervention of feeder cells will bring risk to the clinical use of cell products, especially adopt
The cell for using tumour cell to be cultivated as feeder cells but will bring risk to clinical application.
Utility model content
The utility model does not divide for the feeder cells in existing way requirement incubation and is not proliferated and still keeps
The characteristic of metabolic activity proposes a kind of more spatial cell culture apparatus, to meet above-mentioned requirements.
One aspect according to the present utility model, provides a kind of more spatial cell culture apparatus, including the first culturing room,
It is several to be set to indoor second culturing room of first culture；
First culturing room is equipped with the first bottleneck and covers tightly the first bottle cap of first bottleneck；
Second culturing room is equipped with the second bottleneck and covers tightly the second bottle cap of second bottleneck, and second bottleneck is set
In or stretch out the top of first culturing room；
The wall surface of second culturing room is semi-permeable membrane.
Further, first bottleneck is equipped with external screw thread, and first bottle cap, which is equipped with, matches the externally threaded interior spiral shell
Line is to cooperate first bottle cap to cover tightly the first bottleneck；
Second bottleneck is equipped with external screw thread, and second bottle cap, which is equipped with, matches the externally threaded internal screw thread to cooperate
It states the second bottle cap and covers tightly the second bottleneck.
Further, each second culturing room is fixed in first culturing room by cylinder, and the cylinder is solid
Surely the top and bottom of first culturing room are connected.
Further, first culturing room and the second culturing room are cylinder, and each second culturing room is uniform
It is distributed in the first culturing room.
Further, second culturing room has 4.
Further, first bottle cap and the second bottle cap are closed cover.
Further, first bottle cap and the second bottle cap are ventilating cover.
Further, the bottom of the ventilating cover is equipped with hydrophobic filter membrane.
Further, first bottle cap and the second bottle cap are dottle pin lid.
Further, the bottom of the dottle pin lid is equipped with dottle pin, is reserved with cutting mouth on the dottle pin.
Compared with prior art, the utility model has the beneficial effects that
The utility model is semi-permeable membrane by the way that the second culturing room, the outer wall of the second culturing room are arranged in the first culturing room,
The first culturing room and the second culturing room are equipped with bottleneck and cover tightly the bottle cap of bottleneck simultaneously, thus can basis when coming into operation
The demand of cell culture reasonably arranges each culturing room, cultivates feeder cells in the second culturing room to realize, and the
Aim cell is cultivated in one culturing room.
The additional aspect of the utility model and advantage will be set forth in part in the description, these will be from following description
In become obvious, or recognized by the practice of the utility model.
Detailed description of the invention
The utility model is above-mentioned and/or additional aspect and advantage from the following description of the accompanying drawings of embodiments will
Become obvious and be readily appreciated that, in which:
Fig. 1 is the structure chart of the more spatial cell culture apparatus of one of the utility model embodiment；
Fig. 2 is the top view of the more spatial cell culture apparatus of one of the utility model embodiment；
Fig. 3 is the structure chart of the second culturing room and cylinder in the utility model embodiment；
Fig. 4 is that the bottom of the ventilating cover in the utility model embodiment is equipped with the structure chart of hydrophobic filter membrane；
Fig. 5 is that the bottom of the dottle pin lid in the utility model embodiment is equipped with the sectional view of dottle pin；
Fig. 6 is the schematic diagram of the dottle pin equipped with cutting mouth in the utility model embodiment.
Specific embodiment
In order to make those skilled in the art better understand the scheme of the utility model, below in conjunction with the utility model reality
The attached drawing in example is applied, the technical scheme in the utility model embodiment is clearly and completely described.
In some structures of the description in the specification and claims of the utility model and above-mentioned attached drawing, contain
According to multiple serial numbers that particular order occurs, but it should be clearly understood that serial number such as 1,2 etc., is only used for distinguishing each
Different structures, serial number itself do not represent any meaning.In addition, these processes may include more or fewer operations, and
These operations can be executed in order or be executed parallel.It should be noted that the description such as " first " herein, " second ", is
For distinguishing different message, equipment, module etc., sequencing is not represented, " first " and " second " is not also limited and is different
Type.
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that described embodiment is only the utility model a part example, implement rather than whole
Embodiment.Based on the embodiments of the present invention, those skilled in the art are obtained without creative efforts
The every other embodiment obtained, fall within the protection scope of the utility model.
Those skilled in the art of the present technique are appreciated that unless otherwise defined, all terms used herein (including technology art
Language and scientific term), there is meaning identical with the general understanding of those of ordinary skill in the utility model fields.Also
It should be understood that those terms such as defined in the general dictionary, it should be understood that have and the context of the prior art
In the consistent meaning of meaning otherwise will not use idealization or too formal and unless by specific definitions as here
Meaning is explained.
Embodiment
As shown in Figure 1, providing a kind of more spatial cell culture apparatus of the utility model embodiment, including the first culture
The second culturing room 4 being set in the first culturing room 3 of room 3,4；
First culturing room 3 is cylinder, and the first culturing room 3 is equipped with the first bottleneck 2 and covers tightly first bottle of the first bottleneck 2
Lid, the first bottleneck 2 are equipped with external screw thread, and the first bottle cap is equipped with matching external screw thread to seal the internal screw thread of the first culturing room 3；
Second culturing room 4 is cylinder, and each second culturing room 4 is uniformly and symmetrically distributed in the first culturing room 3；And the
Two culturing room 4 are equipped with the second bottleneck 1 and cover tightly the second bottle cap of the second bottleneck 1, and the second bottleneck 1 is set to or stretches out the first culturing room
3 top, the second bottleneck 1 are equipped with external screw thread, and the second bottle cap is equipped with matching external screw thread to seal the internal screw thread of the second culturing room 4；
The wall surface of second culturing room 4 is semi-permeable membrane；When coming into operation, different cells is respectively put into the first training according to actually required
It supports and is cultivated in the cell growth medium in room 3 and the second culturing room 4, the cell in one of culturing room is during the cultivation process
Growth factor can be secreted, which will penetrate the semi-permeable membrane and enter another culturing room from the culturing room.
As shown in figure 3, each second culturing room 4 is fixed in the first culturing room 3 by cylinder 5, cylinder 5 is fixedly connected with the
The top and bottom of one culturing room 3.
First bottle cap and the second bottle cap can be one of closed cover, ventilating cover or dottle pin lid.
When the first bottle cap and the second bottle cap are closed cover, the present embodiment is used for the closed culture of cell, to guarantee thin
Airtightness of born of the same parents' culture apparatus in cell cultivation process.
When the first bottle cap and the second bottle cap are ventilating cover, as shown in figure 4, the ventilating cover includes cover rim 61,71 and of lid body
Bottom 81, bottom 81 be equipped with hydrophobic filter membrane 91, the hydrophobic filter membrane 91 with a thickness of 0.2 μm, to be trained in cell culture apparatus
Exchange when supporting cell for aseptic gas, to reduce the contaminated risk of cell in cell culture apparatus.
When the first bottle cap and the second bottle cap are dottle pin lid, as shown in figure 5, the dottle pin lid includes cover rim 62,72 and of lid body
Bottom 82, bottom 82 are equipped with dottle pin 92,；The first bottle cap is closed in the dottle pin lid lid and when the second bottle cap, dottle pin fitting is fixed on the
On the bottleneck of one bottle cap and the second bottle cap.
As shown in fig. 6, be reserved with cutting mouth 10 on dottle pin 92 for pipette insertion, the insertion of pipette is in order to the
Liquid feeding, imbibition or the operation such as harvest cell from the first culturing room or the second culturing room in one culturing room or the second culturing room,
The setting of cutting mouth 10 can ensure the air contact culture solution only few in aforesaid operations, to reduce cell culture apparatus
In the contaminated risk of cell, maintain cell culture apparatus in gnotobasis.
The utility model is semi-permeable membrane by the way that the second culturing room, the outer wall of the second culturing room are arranged in the first culturing room,
The first culturing room and the second culturing room are equipped with bottleneck and cover tightly the bottle cap of bottleneck simultaneously, thus can basis when coming into operation
The demand of cell culture reasonably arranges each culturing room, cultivates feeder cells in the second culturing room to realize, and the
Aim cell is cultivated in one culturing room.
The above is only some embodiments of the utility model, it is noted that for the common skill of the art
For art personnel, without departing from the principle of this utility model, several improvements and modifications can also be made, these improve and
Retouching also should be regarded as the protection scope of the utility model.
Claims (10)
1. a kind of more spatial cell culture apparatus, which is characterized in that including the first culturing room, it is several be set to first culturing room
The second interior culturing room；
First culturing room is equipped with the first bottleneck and covers tightly the first bottle cap of first bottleneck；
Second culturing room is equipped with the second bottleneck and covers tightly the second bottle cap of second bottleneck, second bottleneck be set to or
Stretch out the top of first culturing room；
The wall surface of second culturing room is semi-permeable membrane.
2. more spatial cell culture apparatus according to claim 1, which is characterized in that first bottleneck is equipped with outer spiral shell
Line, first bottle cap, which is equipped with, matches the externally threaded internal screw thread to cooperate first bottle cap to cover tightly the first bottleneck；
Second bottleneck is equipped with external screw thread, and second bottle cap, which is equipped with, matches the externally threaded internal screw thread to cooperate described the
Two bottle caps cover tightly the second bottleneck.
3. more spatial cell culture apparatus according to claim 1, which is characterized in that each second culturing room passes through
Cylinder is fixed in first culturing room, and the cylinder is fixedly connected with the top and bottom of first culturing room.
4. more spatial cell culture apparatus according to claim 3, first culturing room and the second culturing room are circle
Cylindricality, each second culturing room are evenly distributed in the first culturing room.
5. more spatial cell culture apparatus according to claim 4, which is characterized in that second culturing room has 4.
6. more spatial cell culture apparatus described in -5 any one according to claim 1, which is characterized in that first bottle cap
It is closed cover with the second bottle cap.
7. more spatial cell culture apparatus described in -5 any one according to claim 1, which is characterized in that first bottle cap
It is ventilating cover with the second bottle cap.
8. more spatial cell culture apparatus according to claim 7, which is characterized in that the bottom of the ventilating cover, which is equipped with, dredges
Water filter membrane.
9. more spatial cell culture apparatus described in -5 any one according to claim 1, which is characterized in that first bottle cap
It is dottle pin lid with the second bottle cap.
10. more spatial cell culture apparatus according to claim 9, which is characterized in that the bottom of the dottle pin lid is equipped with
Dottle pin is reserved with cutting mouth on the dottle pin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821156238.3U CN208748113U (en) | 2018-07-20 | 2018-07-20 | A kind of more spatial cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821156238.3U CN208748113U (en) | 2018-07-20 | 2018-07-20 | A kind of more spatial cell culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208748113U true CN208748113U (en) | 2019-04-16 |
Family
ID=66056116
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821156238.3U Active CN208748113U (en) | 2018-07-20 | 2018-07-20 | A kind of more spatial cell culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208748113U (en) |
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2018
- 2018-07-20 CN CN201821156238.3U patent/CN208748113U/en active Active
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