CN207047204U - A kind of Enrichment by Microorganisms acquisition means - Google Patents

Abstract

The utility model discloses a microorganism enrichment device, the device comprises a first vertical pipe and a second vertical pipe, the height of the first vertical pipe and the second vertical pipe are the same and the bottom is connected by a connecting pipe A first closing cover is installed on the top of the first vertical pipe, and a first filter membrane and a second filter membrane are installed inside the first vertical pipe, and the first filter membrane and the second filter membrane constitute a culture chamber, and The cultivation chamber is connected with the aeration pump through the aeration pipeline, and the waste liquid is discharged from the cultivation chamber through a hose, and the hose is electrically connected with the water pump, and a second closing cover is installed inside the second vertical pipe, and the infusion pipe Penetrating through the second closing cover to deliver the culture solution to the inner space of the second vertical pipe, the infusion tube is electrically connected to the infusion valve, the aeration pump, the water pump and the infusion valve are respectively connected to the power supply electrical connection. The utility model can keep the culture liquid sufficient, and is beneficial to the enrichment and cultivation of microorganisms.

Description

A microbial enrichment device

technical field

The utility model relates to the technical field of microorganism cultivation, in particular to a microorganism enrichment device.

Background technique

The cultivation of microorganisms plays a very basic and important role in the fields of biology and medicine, and can be used in various scenarios such as scientific research, new drug development, drug reaction experiments, etc. Therefore, for many universities, research institutes and pharmaceutical manufacturers, microorganisms are rich in Collection devices can be said to be entry-level equipment.

However, in the microbial enrichment device, the continuous reproduction of microorganisms will inevitably lead to the consumption of the culture medium, and artificially increasing the culture medium requires high manpower, and special personnel are required. Obviously, this will bring about a considerable increase in the cost of research institutions and the like.

Utility model content

The purpose of this utility model is to provide a microorganism enrichment device to solve the problems raised in the above-mentioned background technology.

To achieve the above object, the utility model provides the following technical solution: a microbial enrichment device, the device includes a first vertical pipe and a second vertical pipe, the first vertical pipe and the second vertical pipe have the same height and The bottom is connected by a connecting pipe, the top of the first vertical pipe is equipped with a first closing cover, the first vertical pipe is installed with a first filter membrane and a second filter membrane inside, and the first filter membrane and the second filter membrane are The filter membrane constitutes a cultivation chamber, the cultivation chamber is connected to the aeration pump through the aeration pipe, the waste liquid is discharged from the cultivation chamber through a hose, the hose is electrically connected to the water pump, and the second standpipe is installed inside There is a second closed cover, the infusion tube penetrates the second closed cover to deliver the culture solution to the inner space of the second vertical pipe, the infusion tube is electrically connected to the infusion valve, the aeration pump, the water pump and the infusion valve are respectively electrically connected to a power source.

Preferably, the part of the aeration pipeline located in the cultivation chamber is provided with holes.

Preferably, a buoy is installed at the bottom end of the hose.

Preferably, a glass tube is installed inside the first standpipe, the position of the glass tube is lower than the second filter membrane, and there is a resistance wire inside the glass tube, and the resistance wire is electrically connected to the power supply.

Compared with the prior art, the beneficial effect of the utility model is that the bottom of the first vertical pipe and the second vertical pipe are communicated through the connecting pipe, the first vertical pipe is used for cultivating microorganisms, and the second vertical pipe is used for cultivating microorganisms in the first vertical pipe. Microbial culture provides culture medium. Through the principle of the connector, when the height of the culture solution in the first vertical pipe decreases, the second vertical pipe can be supplemented with the culture liquid in the first vertical pipe at any time because it communicates with the first vertical pipe, and there is no need to use manpower for the second vertical pipe. The culture solution in a vertical pipe is added, which greatly reduces the labor cost.

Description of drawings

Fig. 1 is the structural representation of the first embodiment of the utility model;

Fig. 2 is a partial structural top view of the first embodiment of the present utility model;

Fig. 3 is the structural representation of the second embodiment of the utility model;

Fig. 4 is the structural representation of the third embodiment of the utility model;

Fig. 5 is a schematic structural diagram of the fourth embodiment of the present invention.

In the figure: 1-first vertical pipe, 2-second vertical pipe, 3-connecting pipe, 4-first closing cover, 5-first filter membrane, 6-second filter membrane, 7-aeration pump, 8 -Aeration pipeline, 9-Cultivation room, 10-Hole, 11-Resistance wire, 12-Glass tube, 13-Buoy, 14-Hose, 15-Suction pump, 16-Second closure cover, 17-Infusion tube, 18-infusion valve, 19-power supply.

detailed description

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. example. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

Fig. 1 is a schematic structural diagram of the first embodiment of the utility model, and the structure of the utility model will be described in detail below in conjunction with Fig. 1 .

The utility model provides a microbial enrichment device, comprising a first vertical pipe 1 and a second vertical pipe 2, the first vertical pipe 1 and the second vertical pipe 2 are of the same height and the bottom is connected by a connecting pipe 3, the first vertical pipe 1. A first closing cover 4 is installed on the top, and a first filter membrane 5 and a second filter membrane 6 are installed inside the first vertical pipe 1. The first filter membrane 5 and the second filter membrane 6 constitute a cultivation chamber 9, and the cultivation chamber 9 passes through The aeration pipeline 8 is connected with the aeration pump 7, and the culture room 9 discharges the waste liquid through the hose 14, which is electrically connected with the water pump 15, and the second vertical pipe 2 is equipped with a second closing cover 16, and an infusion tube 17 Penetrating through the second closing cover 16 to deliver the culture solution to the inner space of the second vertical pipe 2, the infusion tube 17 is electrically connected to the infusion valve 18, the aeration pump 7, the water pump 15 and the infusion valve 18 are respectively electrically connected to the power supply 19, Holes 10 are provided on the part of the aeration pipeline 8 located in the cultivation chamber 9 .

Fig. 2 is a partial structural top view of the first embodiment of the present invention. In practice, vessels with larger cross-sectional areas are generally used to culture microorganisms. And the second vertical pipe 2 is to add the culture solution to the first vertical pipe 1, when in the microbial culture process, the consumption of the culture solution is a slow process, so the cross-sectional area of the second vertical pipe 2 does not need The setting is huge. That is to say, Fig. 2 has shown the size situation of the cross-sectional area of the first standpipe 1 and the second standpipe 2 that can be adopted in actual situation, as the cross-sectional area of the first standpipe 1 can be with commonly used in the laboratory The cross-sectional area of the petri dish for cultivating microorganisms is comparable, and the cross-sectional area of the second vertical pipe 2 can be comparable to that of a common graduated cylinder in the laboratory.

FIG. 3 is a schematic structural view of the second embodiment of the present invention, that is, a buoy 13 is installed at the bottom end of the hose 14 on the basis of the first embodiment in FIG. 1 . The flexible pipe 14 is used to discharge the waste liquid after the reaction of the culture liquid. The water content in the culture liquid containing the waste liquid is relatively high. The bottom end of the flexible pipe 14 is equipped with a buoy 13. Sinking to the bottom to inadvertently aspirate culture solution reduces disturbance to microbial cultivation work.

Fig. 4 is the structure diagram of the third embodiment of the present utility model, that is, on the basis of the first embodiment of Fig. 1, a glass tube 12 is installed inside the first vertical pipe 1, the position of the glass tube 12 is lower than the second filter membrane 6 and There is a resistance wire 11 inside the glass tube 12 , and the resistance wire 11 is electrically connected with a power source 19 . Since the culture of microorganisms is highly sensitive to temperature, it is generally necessary to maintain a constant temperature of the culture medium. The resistance wire 11 after electrification can generate heat and maintain a constant temperature, and the resistance wire 11 can be wrapped with a glass tube 12 to protect the resistance wire, thereby keeping the culture medium at a constant temperature, and microorganisms can be better enriched and reproduced.

Fig. 5 is a schematic structural diagram of the fourth embodiment of the present utility model, that is, the second embodiment in Fig. 3 and the third embodiment in Fig. 4 are combined, the characteristics and advantages of the two embodiments can be integrated, and better Provide a favorable environment for the enrichment and reproduction of microorganisms.

Working principle: the culture chamber 9 formed by the space between the first filter membrane 5 and the second filter membrane 6 is used for the cultivation of microorganisms, the aeration pump 7 provides oxygen for the cultivation and enrichment through the aeration pipe 8 and the hole 10, and the hose 14 Under the action of the water pump 15, waste liquid and excess water can be extracted, and the infusion valve 18 can control the delivery of the culture solution by the infusion tube 17. The height of the second closure cover 16 can be adjusted according to the height of the nutrient solution required by microorganisms in the first vertical pipe 1, and then the height of the nutrient solution in the first vertical pipe 1 is controlled to be constant by the height adjustment of the second closure cover 16, Keep the culture medium needed in the microbial enrichment process sufficient.

Although the embodiments of the present invention have been shown and described, those skilled in the art can understand that various changes and modifications can be made to these embodiments without departing from the principle and spirit of the present invention , replacements and modifications, the scope of the present utility model is defined by the appended claims and their equivalents.

Claims (4)

1. A kind of 1. Enrichment by Microorganisms acquisition means, it is characterised in that：Described device includes the first vertical tube (1) and the second vertical tube (2), described First vertical tube (1) is highly identical with second vertical tube (2) and bottom is connected by communicating pipe (3), at the top of first vertical tube (1) First closing cap (4) is installed, the first filter membrane (5) and the second filter membrane (6) be installed inside first vertical tube (1), described the One filter membrane (5) and second filter membrane (6) form culturing room (9), and the culturing room (9) passes through aerated conduit (8) and aeration pump (7) it is connected, the culturing room (9) discharges waste liquid by flexible pipe (14), and the flexible pipe (14) is electrically connected with suction pump (15), institute State and the second closing cap (16) is installed inside the second vertical tube (2), woven hose (17) penetrates second closing cap (16) to described Second vertical tube (2) inner space conveys nutrient solution, and the woven hose (17) is electrically connected with fluid-delivery valve (18), the aeration pump (7), the suction pump (15) and the fluid-delivery valve (18) are electrically connected with power supply (19) respectively.
2. A kind of 2. Enrichment by Microorganisms acquisition means according to claim 1, it is characterised in that：The aeration tube road (8) is located at institute State and hole (10) is provided with the pipe section in culturing room (9).
3. A kind of 3. Enrichment by Microorganisms acquisition means according to claim 1, it is characterised in that：Flexible pipe (14) bottom is provided with Buoy (13).
4. A kind of 4. Enrichment by Microorganisms acquisition means according to claim 1, it is characterised in that：Pacify inside first vertical tube (1) Equipped with glass tube (12), glass tube (12) position, which is less than in second filter membrane (6) and the glass tube (12), resistance Silk (11), the resistance wire (11) are electrically connected with the power supply (19).