CN212924994U

CN212924994U - Box formula photobioreactor that can superpose

Info

Publication number: CN212924994U
Application number: CN202021603134.XU
Authority: CN
Inventors: 梁钧
Original assignee: Xinjiang Jinzheng Biotechnology Co ltd
Current assignee: Xinjiang Jinzheng Biotechnology Co ltd
Priority date: 2020-08-06
Filing date: 2020-08-06
Publication date: 2021-04-09
Anticipated expiration: 2030-08-06

Abstract

The utility model provides a box formula photobioreactor can superpose. The technical scheme who adopts is, including totally closed box formula cultivation pond, the bottom of cultivateing the pond is fixed with the support frame that can arrange other cultivation pond top surfaces in, cultivates and is equipped with introduction port, air inlet, gas outlet, sample connection on the pond, cultivates the top in pond and installs detachable LED lamp strip, and LED lamp strip is connected with the light intensity regulator. The box type culture pond is totally closed, so that the problems of pollution among algae and environmental pollution are avoided; the supporting frame is additionally arranged and used for supporting the box body type culture ponds to play a role in stabilizing, two or more box body type culture ponds are overlapped through the supporting frame, a space is reserved, operation is facilitated when a plurality of box body type culture ponds are overlapped, and the utilization efficiency of the space is improved; install detachable LED lamp strip additional to dispose the light intensity regulator, can adjust the light intensity, satisfied the different growth periods of chlorella to irradiant demand.

Description

Box formula photobioreactor that can superpose

Technical Field

The utility model relates to an alga cultivates technical field, specifically is a box formula photobioreactor that can superpose.

Background

Chlorella is a kind of unicellular green algae of the genus Chlorella of the order Ascophyllaceae of the order Chlorococcales of the phylum Chlorophyta. Chlorella is distributed in various regions of the world, 15 species of the Chlorella are known at present, and the variety of the Chlorella can reach hundreds. Researches find that the chlorella has high protein content and balanced nutrition, and some chlorella can be used for producing high-value products such as amino acid, vitamin, protein and the like. The chlorella in desert is a chlorella strain screened from desert, is more suitable for extreme environment compared with common chlorella, is suitable for high-light and high-temperature conditions in Xinjiang, and is suitable for industrial culture.

Research and research on chlorella are deepened in the fields of biological medicines, biological energy sources, foods, health care products and the like, and a plurality of chlorella products such as health care products, cosmetics and the like are developed. Meanwhile, the content of unsaturated fatty acid in chlorella can be increased through condition induction, and the chlorella can be used for producing biodiesel.

At present, indoor cultivation has a plurality of problems, such as limited space in a laboratory, low effective utilization rate, high illumination required in the cultivation process, light shielding phenomenon in the later stage of microalgae cultivation, high algae liquid density capable of obstructing light transmission, open cultivation easily causing algae liquid pollution and the like.

SUMMERY OF THE UTILITY MODEL

In view of the problems in the prior art, the utility model provides a superposable box type photobioreactor. The technical scheme who adopts is, including totally closed box formula cultivation pond, the bottom of cultivateing the pond is fixed with the support frame that can arrange other cultivation pond top surfaces in, cultivates and is equipped with introduction port, air inlet, gas outlet, sample connection on the pond, cultivates the top in pond and installs detachable LED lamp strip, and LED lamp strip is connected with the light intensity regulator.

Preferably, be equipped with the air disk that is located the bottom in the culture pond, be equipped with a plurality of apertures that are used for leading to mixed air on the air disk, the air disk with the air inlet intercommunication, the gas outlet is located the top of culture pond.

Preferably, the vent pipes connected with the outside of the air inlet and the air outlet are both connected with air valves with sterilization membranes.

Preferably, a plurality of LED lamp strips are uniformly arranged on the top of the culture pond.

Preferably, the culture container is made of acrylic materials.

Preferably, the culture pond is the cuboid structure, the support frame includes the rectangle frame and is located four landing legs of rectangle frame four corners below.

Preferably, the support frame is made of stainless steel.

The utility model has the advantages that: the box type culture pond is totally closed, so that the problems of pollution among algae and environmental pollution are avoided; the supporting frame is additionally arranged and used for supporting the box body type culture ponds to play a role in stabilizing, two or more box body type culture ponds are overlapped through the supporting frame, a space is reserved, operation is facilitated when a plurality of box body type culture ponds are overlapped, and the utilization efficiency of the space is improved; the detachable LED lamp strip is additionally arranged, and the light intensity regulator is configured, so that the light intensity can be regulated, and the requirements of chlorella on illumination in different growth periods are met; when the chlorella culture medium is used for indoor culture, illumination and ventilation conditions required by chlorella growth can be simulated, interference of external factors is avoided, and laboratory research is facilitated.

Drawings

FIG. 1 is a schematic structural view of the stackable box type photobioreactor of the present invention;

in the figure: 1-culture pool, 2-sample inlet, 3-gas inlet, 4-gas outlet, 5-gas disc, 6-sample inlet, 7-LED lamp strip and 8-support frame.

Detailed Description

The technical solution of the present invention will be described in detail by the following specific examples.

As shown in figure 1, the utility model discloses a can superpose box formula and cultivate pond device, including totally closed box formula cultivation pond 1, introduction port 2, air inlet 3, gas outlet 4, air disk 5, sample connection 6, detachable LED lamp strip 7 and support frame 8. The support frame 8 is fixed in the bottom of culture pond 1, arranges the top surface of other culture ponds in when stacking, and the distance between two box formula culture ponds 1 after the equipment is the height of support frame 8. 4 detachable LED lamp strips 7 are pre-installed on the top surface of the culture pond 1, each LED lamp strip 7 is connected with a light intensity regulator, so that the light intensity can be better regulated, and the requirements of chlorella on light intensity in different stages are met. The support frame 8 material is the stainless steel, including rectangle frame and four landing legs that are located rectangle frame four corners below for support box formula and cultivate pond 1 and reserve the space between two box formula cultivation ponds, make things convenient for culture medium to pour into, inoculate, cultivate operations such as sample.

Preferably, the vent pipes connected with the outside of the air inlet 3 and the air outlet 4 are provided with air valves with sterilization films, so that the pollution of external microorganisms is avoided. The gas disk 5 is provided with a plurality of small holes, so that the introduced gas is distributed more uniformly, and meanwhile, the algae liquid is suspended and the illumination is more uniform.

In the embodiment, the high-transparency box-type culture pond 1 is 100cm long, 50cm wide and 35cm high, is used for culturing chlorella, is internally disinfected and sterilized by hypochlorous acid, is made of acrylic, is hard in texture and has high light transmittance. The sample inlet 2 is sealed by a cover, has a diameter of 6cm, and is used for cleaning containers, filling culture solution and inoculating seed solution, and the sample inlet 2 is provided with a small water pump to facilitate the addition of the liquid. The diameters of the air inlet 3 and the air outlet 4 are 5mm, the outside of the air inlet is exposed by 3cm, the air inlet and the air outlet pipe are conveniently inserted, and meanwhile, the air inlet 3 is connected with an air pump through an air pipe and used for introducing CO₂And O₂Or clean air, and the air outlet 4 is used for discharging waste gas to ensure the balance of the internal air pressure. The diameter of the gas disk 5 is 40cm, the vent pipe of the gas disk is connected to the gas inlet 3 and is arranged at the bottom of the inner side of the box type culture pond, and the gas disk 5 is used for introducing mixed gas to ensure that the gas is uniformly distributed and simultaneously the algae cells are suspended to receive light more uniformly. The sampling port 6 is 6cm in diameter and 5cm in length and is used for sampling at intervals and discharging algae liquid and cleaning wastewater in the culture process. The width of the LED lamp strip 7 is 2cm, the length of the LED lamp strip is 50cm, the LED lamp strip can be detached and additionally installed, and a corresponding light intensity regulator is arranged to control the light intensity. The height of the support frame 8 below each box type culture pond is 35cm, and even if the density of the chlorella is increased at the later growth stage, the light transmittance of the device can still meet the requirement of the small ballsThe growth needs of algae. The two box culture ponds are overlapped through the support frames, sufficient operation space is reserved at the same time, and the space utilization rate is improved. The device is equipped with an autoclave for sterilizing culture medium at 121 deg.C under 0.15MPa for 15-20 min.

Before the culture of the desert chlorella, hypochlorous acid is added into the box-type culture pond 1 with high transparency for disinfection and sterilization, and after the disinfection and sterilization, other ports except the sample inlet 6 are closed. Adding 150L of culture medium sterilized by high pressure steam at 121 deg.C for 15-20min through sample inlet 2, opening gas outlet 4, cooling to about 40 deg.C, closing gas valve of gas outlet 4, introducing algae solution at volume ratio of 10%, and sealing sample inlet 6. Open air inlet 3 and gas outlet 4 (cultivate the process and open entirely), use the air pump to let in to 1 kind of required gas of cultivation in box formula cultivation pond through air inlet 3, the gas dish 5 makes gas and culture solution contact more abundant, increases operation rate, and unnecessary gas is discharged via gas outlet 4 simultaneously, keeps the atmospheric pressure balance of 1 kind of in box formula cultivation pond. Adjusting the temperature of the culture environment to 28 ℃ by an air conditioner, adjusting the illumination intensity to 8000lux, culturing for 15 days, and collecting the algae liquid after the culture is finished.

The utility model discloses can also be used to other algal species that are fit for different light intensities of laboratory indoor cultivation, under the condition of the inventive idea and the essence of not changing this experimental apparatus, familiar with relevant technical personnel in this field can carry out the detail change according to its specific production cultivation needs to adapt to its demand, but the change of detail and shape all should belong to the claimed scope of the utility model.

Claims

1. The utility model provides a can superpose box formula photobioreactor which characterized in that: including totally closed box formula cultivation pond, the bottom of cultivateing the pond is fixed with the support frame that can arrange below cultivation pond top surface in, cultivates and is equipped with introduction port, air inlet, gas outlet, sample connection on the pond, cultivates the top in pond and installs detachable LED lamp strip, and LED lamp strip is connected with the light intensity regulator.

2. A stackable box type photobioreactor as claimed in claim 1, wherein: be equipped with the air disk that is located the bottom in the culture pond, be equipped with a plurality of apertures that are used for leading to mixed air on the air disk, the air disk with the air inlet intercommunication, the gas outlet is located the top of culture pond.

3. A stackable box type photobioreactor apparatus as claimed in claim 1 or 2, wherein: air valves with sterilization membranes are connected on the air vent pipes externally connected with the air inlet and the air outlet.

4. A stackable box type photobioreactor as claimed in claim 1, wherein: and a plurality of LED lamp strips are uniformly arranged at the top of the culture pond.

5. A stackable box type photobioreactor as claimed in claim 1, wherein: the culture pond is made of acrylic materials.

6. A stackable box type photobioreactor as claimed in claim 1, wherein: the culture pond is the cuboid structure, the support frame includes the rectangle frame and is located four landing legs of rectangle frame four corners below.

7. A stackable box type photobioreactor according to claim 1 or 6, wherein: the support frame is made of stainless steel.