CN203329089U - Device for preparing large-area amniotic membrane patch loaded with stem cells - Google Patents
Device for preparing large-area amniotic membrane patch loaded with stem cells Download PDFInfo
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- CN203329089U CN203329089U CN2012206889330U CN201220688933U CN203329089U CN 203329089 U CN203329089 U CN 203329089U CN 2012206889330 U CN2012206889330 U CN 2012206889330U CN 201220688933 U CN201220688933 U CN 201220688933U CN 203329089 U CN203329089 U CN 203329089U
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- amniotic membrane
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Abstract
The utility model relates to a device for preparing a large-area amniotic membrane patch loaded with stem cells. The device for preparing the large-area amniotic membrane patch loaded with stem cells is characterized by comprising a cell culture dish and a horizontal support horizontally arranged inside the cell culture dish, wherein upper limit of the size of the horizontal support is close to the size of the cell culture dish to the greatest extent and is within the limit that the horizontal support can be placed into the cell culture dish after being paved with an amniotic membrane, the depth of the cell culture dish is within the limit that the upper surface of a horizontal table top of the horizontal support is at least 1cm lower than the upper edge of the wall of the cell culture dish in vertical height after the horizontal support tightly attached with an amniotic membrane original film is placed, and the diameter of the cell culture dish is 9-26cm. The device provided by the utility model can be used for preparing the large-area amniotic membrane patch loaded with the stem cells, so that a necessary material is provided for covering an early wound of an extensive burn and a wound surface.
Description
Technical field
This utility model relates to the preparation facilities of the large tracts of land amniotic membrane paster of a kind of load stem cell.
Background technology
Skin is the barrier that human body contacts with external environment, not only plays the effects such as protection, secretion, metabolism and sensation, and participates in immunoreation and maintain the stable of organismic internal environment.So far, the skin source of severe trauma and Patients with Big Area Burn shortage is a clinical difficult problem urgently to be resolved hurrily always.The success of organization engineering skin product development, make the research of artificial skin realize qualitative leap.At present, gone on the market and be applied to clinical organization engineering skin product Dermagraft is arranged
and ActivSkin
deng, certain clinical effectiveness is arranged, but still have some shortcomings part and price very expensive.
Early stage burn wound covers can restricting bacterial growth, prevention infection, prevent moisture and electrolytical loss.Auto-skin grafting is the first-selection for the treatment of burn, but, for large-area burns, autologous skin can not satisfy the demands, and heterogenous skin can cause obvious immunologic rejection.Thereby the research of tissue engineering skin has become one of the focus in life science field.
People's amniotic membrane is translucent thin film, without blood vessel, nerve and lymphatic vessel, the nutritional labeling of multiple promotion cell proliferation is arranged, and does not express the human leukocyte antigen, has anti-microbial effect and adhesiveness preferably.The existing amnion transplantation thing of using is treated a report of table damage disease at present.But the amniotic membrane that the many employings of prior art are directly peeled off, without any processing.Some researcheres on described amniotic membrane load autologous bone marrow mesenchymal stem cells.Because the eye area is less, so the amniotic membrane paster of load cells is easy to be prepared.But the preparation of the amniotic membrane paster of large tracts of land load cells is because the receptor restriction is immersed in the surface irregularity in culture medium with it, and cell is difficult for growth, and then its application is restricted.
Although also have on the amniotic membrane of 3cm diameter and be affixed on basal surface with nitrocellulose filter as support in prior art, make the report of paster for the cornea damage, this diaphragm is less, can not the large skin injury of area coverage.So far, at diameter, be not also to plant the report of cell on the llama film paster more than 10cm.Because a condition of cell attachment growth is that the smooth surface that needs will be adherent is smooth, and amniotic membrane is in the situation that in being dipped into culture fluid without holder the time, can be due to buoyancy by puff, cause surperficial more fold, make cell be difficult for adherent and and then affect its growth and propagation.Simultaneously, amniotic membrane should be able to easily and entirely be fixed on holder, and is placed in Tissue Culture Dish.For the large tracts of land amniotic membrane paster of the load stem cell that makes to obtain, can try one's best when the application complete and closely with the wound surface laminating, should keep the smooth and complete of former of amniotic membrane when being fixed on holder by former of amniotic membrane as far as possible.
Therefore, in order to prepare the large tracts of land amniotic membrane paster of the load stem cell that meets above-mentioned requirements, need a kind of new supporting preparation facilities of development.
The utility model content
Technical problem to be solved in the utility model is: when the diameter of preparation load stem cell is the llama film paster more than 10cm, a condition of growing due to cell attachment is that the smooth surface that needs will be adherent is smooth, and amniotic membrane is in the situation that in being dipped into culture fluid without holder the time, can be due to buoyancy by puff, cause surperficial more fold, make cell be difficult for adherent and and then affect its growth and propagation, simultaneously, amniotic membrane should be able to easily and entirely be fixed on holder, and is placed in Tissue Culture Dish.For the large tracts of land amniotic membrane paster of the load stem cell that makes to obtain, can try one's best when the application complete and closely with the wound surface laminating, should keep the smooth and complete of former of amniotic membrane when being fixed on holder by former of amniotic membrane as far as possible.
For achieving the above object, this utility model provides the preparation facilities of the large tracts of land amniotic membrane paster of a kind of load stem cell, it is characterized in that its horizontal holder be placed in one by Tissue Culture Dish and level forms, be limited on the size of wherein said horizontal holder with the size of Tissue Culture Dish and approach as far as possible, can insert Tissue Culture Dish and be limited to spread after amniotic membrane, the degree of depth of described Tissue Culture Dish is with after inserting the tight horizontal holder that has attached former of amniotic membrane, the upper surface of the level table of described horizontal holder on vertical height lower than being limited along 1cm at least on the limit wall of Tissue Culture Dish, the diameter of described Tissue Culture Dish is 9-26cm.
In preferred embodiment, the shape of described Tissue Culture Dish is identical with the shape of described holder, further, and described circle, square, the rectangle of being shaped as, further, the described circle that is shaped as.The Tissue Culture Dish that described Tissue Culture Dish is general commercial; The material glass of described horizontal holder.Certainly, the material of described Tissue Culture Dish there is no specific limited, as long as it can be grown by sustenticular cell, the material of described horizontal holder can be also plastics.The thickness of the horizontal plane of described horizontal holder is 1-4mm, and further, thickness is 1.5mm.
In preferred embodiment, the one side down of the horizontal plane of described horizontal holder is fixed with the thrust for former of auxiliary fixedly amniotic membrane, the horizontal plane size that described thrust stably supports described horizontal holder and described thrust is less than the size of the horizontal plane of horizontal holder, and described thrust is that the horizontal plane of its horizontal plane and horizontal holder is concentrically ringed circular thrust.
Perhaps, in another preferred embodiment, the one side down of the level table of described horizontal holder is fixed with the thrust for former of auxiliary fixedly amniotic membrane, the horizontal plane size that described thrust stably supports described horizontal holder and described thrust is less than the size of the horizontal plane of horizontal holder, described thrust be form plane and make horizontal holder reposefully level be placed at least 3 bolt shape thrusts of culture dish.
In preferred embodiment, the area of described horizontal holder is 200cm
2.Certainly, according to varying in size of Tissue Culture Dish used, the area of described horizontal holder can be 80cm
2-500cm
2.
In preferred embodiment, the edge of the horizontal plane of described horizontal holder carries out smooth to guarantee, smooth, the unpolarized burr of grinding process.
Certainly, the material of described horizontal holder is preferably glass and does not carry out the silanization processing.
Below in conjunction with said structure of the present utility model, its useful technique effect is described: the preparation facilities of the large tracts of land amniotic membrane paster of load stem cell of the present utility model has successfully been realized when the diameter for preparing the load stem cell is the llama film paster more than 10cm by the horizontal holder of placing fixing amniotic membrane paster in Tissue Culture Dish, for the cell attachment growth provides necessary smooth planar surface, simultaneously, utilize preparation facilities amniotic membrane of the present utility model easily and entirely to be fixed on holder, and be placed in Tissue Culture Dish.The large tracts of land amniotic membrane paster of the load stem cell obtained can be complete and closely with wound surface, fit in when application.The large tracts of land amniotic membrane paster that utilizes preparation facilities of the present utility model to prepare to plant stem cell, provide necessary medical material for the early stage wound surface of large-area burns and severe trauma patients covers.
The accompanying drawing explanation
The schematic diagram of the simple realization form of the preparation facilities of the large tracts of land amniotic membrane paster that Fig. 1 is load stem cell of the present utility model;
The schematic diagram of one of better way of realization of the preparation facilities of the large tracts of land amniotic membrane paster that Fig. 2 is load stem cell of the present utility model;
Two schematic diagram of the better way of realization of the preparation facilities of the large tracts of land amniotic membrane paster that Fig. 3 is load stem cell of the present utility model;
The using state figure of the preparation facilities of the large tracts of land amniotic membrane paster that Fig. 4 is the load stem cell shown in Fig. 2.
The specific embodiment
Relevant this utility model is for achieving the above object, and the technological means adopted and effect thereof, now enumerate possible embodiments, and coordinates accompanying drawing to be described as follows:
At first, refer to Fig. 1, it illustrates a kind of simple realization mode of preparation facilities of the large tracts of land amniotic membrane paster of load stem cell of the present utility model, wherein the lid 1 of Tissue Culture Dish is placed on the body 4 of Tissue Culture Dish, horizontal holder 3 directly is placed in going to the bottom on 5 of Tissue Culture Dish, and wherein the upper surface of horizontal holder 3 is at least 1cm apart from the vertical dimension on the upper edge of Tissue Culture Dish limit wall 2.Wherein the diameter of Tissue Culture Dish body is 9-26cm, and the diameter of horizontal holder is that 8-25cm, thickness are 1-4mm.In use, former of amniotic membrane flatly is layered on horizontal holder 3 and the former part of amniotic membrane that with cord, former of unnecessary amniotic membrane is tied to guarantee to be layered on horizontal holder 3 in contrary one side is smooth, tight.After horizontal holder 3 levels that will tie former of amniotic membrane are inserted on Tissue Culture Dish body 4, horizontal holder 3(comprises former of the amniotic membrane that covers on it) upper surface be about 1.0cm apart from the vertical dimension on the upper edge of Tissue Culture Dish limit wall 2.This distance is enough to guarantee the operation that adds enough cell culture mediums to cultivate and plant to carry out follow-up stem cell.
Simultaneously, thickness for the horizontal holder in above-mentioned example, although on theory, the holder that thickness is larger is conducive to former of amniotic membrane is fixed thereon as glass plate, but for avoiding adverse effect to follow-up cell culture (when thickness is larger, holder easily disturbs the temperature of culture fluid, and the volume that effective culture medium is arranged in culture dish is reduced, cause the frequent culture fluid of changing of needs, both increased the probability polluted, caused again unnecessary operation), need the thickness of holder in appropriate scope.Simultaneously, in order to keep enough intensity, former of amniotic membrane is being fixed on holder and is damaging holder avoiding, the thickness of holder can not be too low.Through research and probe repeatedly, this utility model finds that thickness is acceptable in the 1-4mm scope, and the holder that thickness is 1.5mm is optimum selection.As for the size of Tissue Culture Dish and horizontal holder, it can be according to the size of the amniotic membrane paster that will obtain and corresponding selection, as long as guaranteed to tie the horizontal holder energy level of former of amniotic membrane, is placed in Tissue Culture Dish.
Fig. 2 has shown a kind of preferably implementation of preparation facilities of the large tracts of land amniotic membrane paster of load stem cell of the present utility model, wherein the lid 1 of Tissue Culture Dish is placed on the body 4 of Tissue Culture Dish, horizontal holder 3 directly is placed in going to the bottom on 5 of Tissue Culture Dish, and wherein the upper surface of horizontal holder 3 is at least 1cm apart from the vertical dimension on the upper edge of Tissue Culture Dish limit wall 2.Wherein the diameter of Tissue Culture Dish body is 9-26cm, and the diameter of horizontal holder is that 8-25cm, thickness are 1-4mm.In this preferred embodiment, the lower surface of described horizontal holder 3 is fixed with the thrust 6 for former of auxiliary fixedly amniotic membrane, the horizontal plane size that described thrust 6 stably supports described horizontal holder 3 and described thrust 6 is less than the size of the horizontal plane of horizontal holder 3, and described thrust 6 is that the horizontal plane of its horizontal plane and horizontal holder 3 is concentrically ringed circular thrust.In use, former of amniotic membrane flatly is layered on horizontal holder 3 and with cord former of unnecessary amniotic membrane is tied on thrust 6 to guarantee that the former part of amniotic membrane be layered on horizontal holder 3 is smooth, tight.After horizontal holder 3 levels that will tie former of amniotic membrane are inserted on Tissue Culture Dish body 4, horizontal holder 3(comprises former of amniotic membrane and the thrust 6 covered on it) upper surface apart from the vertical dimension on the upper edge of Tissue Culture Dish limit wall 2, to guarantee to be not less than into 1.0cm, carry out to guarantee to add enough cell culture mediums that follow-up stem cell is cultivated and the operation of plantation.Due to the existence that thrust 6 is arranged, by amniotic membrane former can utilize while tying on horizontal holder cord more convenient, carry out quickly, and it is tighter to tie ground, the former part of amniotic membrane that makes to be layered on level table is more smooth, non-wrinkled.Because thrust 6 can take the Partial Height of Tissue Culture Dish, the Tissue Culture Dish that therefore can lengthen by customization limit wall 2 meet test required.
Fig. 3 has shown the another kind implementation preferably of preparation facilities of the large tracts of land amniotic membrane paster of load stem cell of the present utility model, the difference of the embodiment shown in itself and Fig. 2 be described thrust 6 for form plane and make horizontal holder reposefully level be placed at least 3 bolt shape thrusts of culture dish.Its using method is also identical with the embodiment shown in Fig. 2.Because the thrust 6 of the integral form in embodiment illustrated in fig. 2 is replaced by least three much smaller bolt shape thrusts of volume, therefore, can load the cell culture medium of more volumes in Tissue Culture Dish, thereby reduce the number of times of changing culture medium and be conducive to keep the stable of whole cell culture environment.
Certainly, those skilled in the art know, and the position of described thrust 6 can be positioned at the center of described horizontal holder 3, also can be positioned at its off-centered position, if can assurance horizontal holder stably level be placed in Tissue Culture Dish.
Fig. 4 has shown the using state figure of the preparation facilities shown in Fig. 2, wherein on horizontal holder, has tied former 7 of amniotic membrane, and former 7 use cords of amniotic membrane are banded on thrust 6.
In sum, preparation facilities of the present utility model has solved defect and the deficiency that can not prepare the large tracts of land amniotic membrane paster of having planted stem cell in the prior art, utilizes device of the present utility model can prepare area and is at least 80cm
2plantation the amniotic membrane paster of stem cell, and the plantation that can prepare the maximum area of amniotic membrane paster of stem cell can reach 500cm
2.Thereby for the getting up early wound cover of large-area burns and wound face provides indispensable material foundation.
Above illustrated embodiment is only for convenient explanation this utility model, and be not in a limitative way, under not by the prerequisite from this utility model spirit category, various simple and easy variation and modification that the those skilled in the art can do, all must be included in the scope of claim.
Claims (10)
1. the preparation facilities of the large tracts of land amniotic membrane paster of a load stem cell, it is characterized in that its horizontal holder be placed in one by Tissue Culture Dish and level forms, the upper limit of the size of wherein said horizontal holder can be inserted Tissue Culture Dish and is limited to spread after amniotic membrane, the degree of depth of described Tissue Culture Dish is with after inserting the tight horizontal holder that has attached former of amniotic membrane, the upper surface of the level table of described horizontal holder is lower than being limited along 1cm at least on the limit wall of Tissue Culture Dish on vertical height, and the diameter of described Tissue Culture Dish is 9-26cm.
2. the preparation facilities of the large tracts of land amniotic membrane paster of load stem cell according to claim 1, is characterized in that the shape of described Tissue Culture Dish is identical with the shape of described holder, described circle, square, the rectangle of being shaped as.
3. the preparation facilities of the large tracts of land amniotic membrane paster of load stem cell according to claim 2, is characterized in that the described circle that is shaped as.
4. according to the preparation facilities of the large tracts of land amniotic membrane paster of the described load stem cell of claims 1 to 3 any one, the material that it is characterized in that described horizontal holder is glass.
5. according to the preparation facilities of the large tracts of land amniotic membrane paster of the described load stem cell of claims 1 to 3 any one, the thickness that it is characterized in that the level table of described horizontal holder is 1-4mm.
6. the preparation facilities of the large tracts of land amniotic membrane paster of load stem cell according to claim 5, the thickness that it is characterized in that the level table of described horizontal holder is 1.5mm.
7. according to the preparation facilities of the large tracts of land amniotic membrane paster of the described load stem cell of claims 1 to 3 any one, the one side down that it is characterized in that the level table of described horizontal holder is fixed with the thrust for former of auxiliary fixedly amniotic membrane, the horizontal plane size that described thrust stably supports described horizontal holder and described thrust is less than the size of the horizontal plane of horizontal holder, and described thrust is that the horizontal plane of its horizontal plane and horizontal holder is concentrically ringed circular thrust.
8. according to the preparation facilities of the large tracts of land amniotic membrane paster of the described load stem cell of claims 1 to 3 any one, the one side down that it is characterized in that the level table of described horizontal holder is fixed with the thrust for former of auxiliary fixedly amniotic membrane, the horizontal plane size that described thrust stably supports described horizontal holder and described thrust is less than the size of the horizontal plane of horizontal holder, described thrust be form plane and make horizontal holder reposefully level be placed at least 3 bolt shape thrusts of culture dish.
9. according to the preparation facilities of the large tracts of land amniotic membrane paster of the described load stem cell of claims 1 to 3 any one, the area that it is characterized in that the level table of described horizontal holder is 200cm
2.
10. according to the preparation facilities of the large tracts of land amniotic membrane paster of the described load stem cell of claims 1 to 3 any one, the edge that it is characterized in that the level table of described horizontal holder carries out grinding process to be made it smooth, smooth and not to have bossed burr.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012206889330U CN203329089U (en) | 2012-12-13 | 2012-12-13 | Device for preparing large-area amniotic membrane patch loaded with stem cells |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012206889330U CN203329089U (en) | 2012-12-13 | 2012-12-13 | Device for preparing large-area amniotic membrane patch loaded with stem cells |
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|---|---|
| CN203329089U true CN203329089U (en) | 2013-12-11 |
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ID=49697793
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012206889330U Expired - Lifetime CN203329089U (en) | 2012-12-13 | 2012-12-13 | Device for preparing large-area amniotic membrane patch loaded with stem cells |
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|---|---|
| CN (1) | CN203329089U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104818244A (en) * | 2015-05-21 | 2015-08-05 | 黑龙江天晴干细胞股份有限公司 | Amnion epithelial cell separation and culture method |
| CN104974980A (en) * | 2015-05-21 | 2015-10-14 | 重庆市细胞生物工程技术有限公司 | Human amniotic epithelial cell separation method |
-
2012
- 2012-12-13 CN CN2012206889330U patent/CN203329089U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104818244A (en) * | 2015-05-21 | 2015-08-05 | 黑龙江天晴干细胞股份有限公司 | Amnion epithelial cell separation and culture method |
| CN104974980A (en) * | 2015-05-21 | 2015-10-14 | 重庆市细胞生物工程技术有限公司 | Human amniotic epithelial cell separation method |
| CN104818244B (en) * | 2015-05-21 | 2017-12-29 | 天晴干细胞股份有限公司 | A kind of amniotic epithelial cells separation, the method for culture |
| CN104974980B (en) * | 2015-05-21 | 2018-06-22 | 重庆市细胞生物工程技术有限公司 | A kind of separation method of human amniotic membrane epithelial cell |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Bi Weiwei Inventor after: Li Chao Inventor before: Bi Weiwei Inventor before: Jiang Lijun |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: BI WEIWEI JIANG LIJUN TO: BI WEIWEI LI CHAO |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20131211 |