CN206902162U - A kind of cell culture cell for epidermis model 3D cultures - Google Patents
A kind of cell culture cell for epidermis model 3D cultures Download PDFInfo
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- CN206902162U CN206902162U CN201720706098.1U CN201720706098U CN206902162U CN 206902162 U CN206902162 U CN 206902162U CN 201720706098 U CN201720706098 U CN 201720706098U CN 206902162 U CN206902162 U CN 206902162U
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- cell
- cell culture
- cultures
- epidermis model
- epidermis
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- 238000004113 cell culture Methods 0.000 title claims abstract description 90
- 210000002615 epidermis Anatomy 0.000 title claims abstract description 74
- 239000012528 membrane Substances 0.000 claims abstract description 24
- 230000035699 permeability Effects 0.000 claims abstract description 7
- 230000009286 beneficial effect Effects 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 235000016709 nutrition Nutrition 0.000 claims description 3
- 230000035764 nutrition Effects 0.000 claims description 3
- 239000004417 polycarbonate Substances 0.000 claims description 3
- 229920000515 polycarbonate Polymers 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 abstract description 71
- 210000002201 cell of epidermis Anatomy 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 210000001339 epidermal cell Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model embodiment provides a kind of cell culture cell for epidermis model 3D cultures, it is related to bioengineering field, the structure that 3D epidermis models are completed for the cell culture cell of epidermis model 3D cultures can be utilized, and the growth conditions and mode of appearance of convenient observation epidermis model in cell cultivation process, while it is easy to experimenter to operate the cell culture cell cultivated for epidermis model 3D.The cell culture cell for being used for epidermis model 3D cultures has permeability, and the cell culture cell for epidermis model 3D cultures includes：Cell culture cell cup, the filter membrane of cell culture cell bottom of cup is arranged at, three pillars being connected with cell culture cell bottom of cup, and it is arranged on the incline of at least two equal lengths of cell culture cell cup lateral surface.
Description
Technical field
It the utility model is related to bioengineering field, more particularly to a kind of cell culture for epidermis model 3D cultures is small
Room.
Background technology
Cell culture cell is a kind of labware for cell culture.The side one of traditional cell culture cell
As be smooth or have an a plurality of rib, however, during epidermis model 3D is cultivated, the cell of smooth-sided compression candles can not be fixed
On mould, and there is the cell of a plurality of rib to be not easy to experimenter for side that it is shifted with tweezers.
The content of the invention
The utility model embodiment provides a kind of cell culture cell for epidermis model 3D cultures.It can utilize and use
The structure of 3D epidermis models, and the convenient sight in cell cultivation process are completed in the cell culture cell of epidermis model 3D cultures
The growth conditions and mode of appearance of epidermis model are examined, while are easy to cell culture of the experimenter to being cultivated for epidermis model 3D
Cell is operated.
To reach above-mentioned purpose, the utility model embodiment adopts the following technical scheme that：
The utility model embodiment provides a kind of cell culture cell for epidermis model 3D cultures, for epidermis model
The cell culture cell of 3D cultures has permeability, and the cell culture cell for epidermis model 3D cultures includes：Cell culture
Cell cup, the filter membrane of cell culture cell bottom of cup is arranged at, three to be connected with cell culture cell bottom of cup
Individual pillar, and it is arranged on the incline of at least two equal lengths of cell culture cell cup lateral surface.
Preferably, the lateral surface of cell culture cell cup is provided with the incline of three equal lengths.
Optionally, the incline of three equal lengths corresponds respectively at three pillars.
Further, cell culture cell cup is used to provide nutrient solution for the first stage of epidermis model 3D cultures, and
The growth conditions and mode of appearance of epidermis model are observed in incubation.
Further, incline is used for after being put into mould in the cell culture cell cultivated for epidermis model 3D, ensures filter
Height residing for film.
Further, filter membrane is treated polycarbonate membrane, for making cell easily attach filter membrane growth, and profit
Nutrition is absorbed in epidermis model.
Further, three pillars are used for the planarization for ensureing filter membrane, and in epidermis model transportation, beneficial to general
Cell culture cell for epidermis model 3D cultures is fixed on gel entrapment culture base.
Preferably, the aperture of filter membrane is 0.4 μm.
The utility model provides a kind of cell culture cell for epidermis model 3D cultures, is trained for epidermis model 3D
Foster cell culture cell has permeability, and the cell culture cell for epidermis model 3D cultures includes：Cell culture cell
Cup, the filter membrane of cell culture cell bottom of cup is arranged at, three branch being connected with cell culture cell bottom of cup
Post, and it is arranged on the incline of at least two equal lengths of cell culture cell cup lateral surface.It can utilize and be used for epidermis
Complete the structure of 3D epidermis models, and the convenient observation epidermis in cell cultivation process in the cell culture cell of model 3D cultures
The growth conditions and mode of appearance of model, while it is easy to experimenter to enter the cell culture cell cultivated for epidermis model 3D
Row operation.
Brief description of the drawings
Fig. 1 is a kind of structure for cell culture cell for epidermis model 3D cultures that the utility model embodiment provides
Schematic diagram one；
Fig. 2 is a kind of structure for cell culture cell for epidermis model 3D cultures that the utility model embodiment provides
Schematic diagram two；
Fig. 3 is a kind of cell culture cell for epidermis model 3D cultures that the utility model embodiment Fig. 2 is provided
Top view；
Fig. 4 is a kind of structure for cell culture cell for epidermis model 3D cultures that the utility model embodiment provides
Schematic diagram three；
Fig. 5 is a kind of cell culture cell for epidermis model 3D cultures that the utility model embodiment Fig. 4 is provided
Top view.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Describe in detail.
The utility model embodiment provides a kind of cell culture cell for epidermis model 3D cultures, and this is used for epidermis mould
The structure chart of the cell culture cell of type 3D cultures is as shown in figure 1, this is used for the cell culture cell tool of epidermis model 3D cultures
There is permeability, i.e. the cell culture cell for epidermis model 3D cultures can be made of transparent material, such as transparent
Plastics, glass etc..
Cell culture cell for epidermis model 3D cultures includes：Cell culture cell cup 10, it is arranged at cell training
Support the filter membrane 11 of the bottom of cell cup 10, three pillars 12, Yi Jishe being connected with the bottom of cell culture cell cup 10
Put the incline 13 at least two equal lengths of the lateral surface of cell culture cell cup 10（As shown in Figure 1 is used for epidermis mould
The cell culture cell of type 3D cultures includes the incline 13 of four equal lengths）.
Preferably, as shown in Fig. 2 the lateral surface of cell culture cell cup 10 is provided with the incline of three equal lengths
13.Wherein, the top view of the cell culture cell for being used for epidermis model 3D cultures shown in Fig. 2 is as shown in Figure 3.
Further, in a kind of possible implementation, as shown in figure 4, the incline 13 of three equal lengths respectively at
Three pillars 12 spatially correspond.Wherein, the cell culture cell for being used for epidermis model 3D culture shown in Fig. 4 is bowed
View is as shown in Figure 5.
Further, cell culture cell cup 10 is used to provide nutrient solution for the first stage of epidermis model 3D cultures,
And the growth conditions and mode of appearance of the epidermis model are observed in incubation.
Further, incline 13 is used for after being put into mould in the cell culture cell cultivated for epidermis model 3D, ensures
Height residing for filter membrane 11.
Meanwhile incline 13 also helps and the cell culture cell cultivated for epidermis model 3D is fixed in mould.Together
When, in the case that incline 13 only has three, can solve the problems, such as, because incline is not easy tweezers gripping too much, to facilitate for table
The transfer of the cell culture cell of skin model 3D cultures, it is simple and practical, brought great convenience to operating process.
Further, filter membrane 11 is treated polycarbonate membrane, is grown for making cell easily attach filter membrane 11, and
And absorb nutrition beneficial to epidermis model.
Preferably, the aperture of filter membrane 11 is 0.4 μm.
It should be noted that in the building process of 3D epidermis models, have and gentle liquid level culture is cultivated under liquid, cultivated under liquid
When, filter membrane 11 can grow for cell attachment provides supporter；When being cultivated in gas-liquid face, epidermal cell can pass through filter membrane 11
Permeability draws nourishment under film in nutrient solution.
Further, three pillars 12 are used for the planarization for ensureing filter membrane 11, and in epidermis model transportation, profit
In the cell culture cell cultivated for epidermis model 3D is fixed on into gel entrapment culture base.
The utility model provides a kind of cell culture cell for epidermis model 3D cultures, is trained for epidermis model 3D
Foster cell culture cell has permeability, and the cell culture cell for epidermis model 3D cultures includes：Cell culture cell
Cup, the filter membrane of cell culture cell bottom of cup is arranged at, three branch being connected with cell culture cell bottom of cup
Post, and it is arranged on the incline of at least two equal lengths of cell culture cell cup lateral surface.It can utilize and be used for epidermis
Complete the structure of 3D epidermis models, and the convenient observation epidermis in cell cultivation process in the cell culture cell of model 3D cultures
The growth conditions and mode of appearance of model, while it is easy to experimenter to enter the cell culture cell cultivated for epidermis model 3D
Row operation.
Described above, the only embodiment of the application, but the protection domain of the application is not limited thereto is any
Change or replacement in the technical scope that the application discloses, should all cover within the protection domain of the application.Therefore, this Shen
Protection domain please should be based on the protection scope of the described claims.
Claims (8)
1. a kind of cell culture cell for epidermis model 3D cultures, it is characterised in that the epidermis model 3D that is used for is cultivated
Cell culture cell there is permeability, the cell culture cell for epidermis model 3D cultures includes：Cell culture is small
Room cup, the filter membrane of cell culture cell bottom of cup is arranged at, be connected with cell culture cell bottom of cup
Three pillars connect, and it is arranged on the incline of at least two equal lengths of the cell culture cell cup lateral surface.
2. the cell culture cell according to claim 1 for epidermis model 3D cultures, it is characterised in that the cell
The lateral surface of culture cell cup is provided with the incline of three equal lengths.
3. the cell culture cell according to claim 2 for epidermis model 3D cultures, it is characterised in that described three
The incline of equal length corresponds respectively at three pillars.
4. the cell culture cell for being used for epidermis model 3D cultures according to any one in claim 1-3, its feature
It is, cell culture cell cup is used to provide nutrient solution for the first stage of epidermis model 3D cultures, and is cultivating
The growth conditions and mode of appearance of the epidermis model are observed in journey.
5. the cell culture cell for being used for epidermis model 3D cultures according to any one in claim 1-3, its feature
It is, the incline is used for after being put into mould in the cell culture cell for epidermis model 3D cultures, ensures the filter
Height residing for film.
6. the cell culture cell for being used for epidermis model 3D cultures according to any one in claim 1-3, its feature
It is, the filter membrane is treated polycarbonate membrane, for making cell easily attach the filter membrane growth, and is beneficial to institute
State epidermis model and absorb nutrition.
7. the cell culture cell for being used for epidermis model 3D cultures according to any one in claim 1-3, its feature
It is, three pillars are used for the planarization for ensureing the filter membrane, and in the epidermis model transportation, beneficial to general
The cell culture cell for epidermis model 3D cultures is fixed on gel entrapment culture base.
8. the cell culture cell for being used for epidermis model 3D cultures according to any one in claim 1-3, its feature
It is, the aperture of the filter membrane is 0.4 μm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720706098.1U CN206902162U (en) | 2017-06-18 | 2017-06-18 | A kind of cell culture cell for epidermis model 3D cultures |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720706098.1U CN206902162U (en) | 2017-06-18 | 2017-06-18 | A kind of cell culture cell for epidermis model 3D cultures |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206902162U true CN206902162U (en) | 2018-01-19 |
Family
ID=61284780
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720706098.1U Active CN206902162U (en) | 2017-06-18 | 2017-06-18 | A kind of cell culture cell for epidermis model 3D cultures |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206902162U (en) |
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2017
- 2017-06-18 CN CN201720706098.1U patent/CN206902162U/en active Active
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240119 Address after: 215558 Southeast Avenue, Changshu High tech Industrial Development Zone, Suzhou City, Jiangsu Province Patentee after: Boxi Biotechnology (Suzhou) Co.,Ltd. Address before: 523808 room 08, 1 / F, cooperation center, Dongguan Taiwan Biotechnology Center, No.1 Taoyuan Road, Taiwan hi tech park, Songshanhu hi tech Industrial Development Zone, Dongguan City, Guangdong Province Patentee before: GUANGDONG BIOCELL BIOTECHNOLOGY Co.,Ltd. |