CN205681918U - A kind of vaccination mechanism for cultivating edible fungi and strain container - Google Patents
A kind of vaccination mechanism for cultivating edible fungi and strain container Download PDFInfo
- Publication number
- CN205681918U CN205681918U CN201620579488.2U CN201620579488U CN205681918U CN 205681918 U CN205681918 U CN 205681918U CN 201620579488 U CN201620579488 U CN 201620579488U CN 205681918 U CN205681918 U CN 205681918U
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- inoculation
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- 241000233866 Fungi Species 0.000 title claims abstract description 55
- 238000002255 vaccination Methods 0.000 title claims abstract description 45
- 238000011081 inoculation Methods 0.000 claims abstract description 130
- 239000001963 growth media Substances 0.000 claims abstract description 39
- 235000015097 nutrients Nutrition 0.000 claims abstract description 7
- 241000894006 Bacteria Species 0.000 claims description 20
- 239000004746 geotextile Substances 0.000 claims description 5
- 210000003284 Horns Anatomy 0.000 claims description 2
- 238000001914 filtration Methods 0.000 abstract description 29
- 238000004519 manufacturing process Methods 0.000 abstract description 29
- 230000001954 sterilising Effects 0.000 description 14
- 235000016709 nutrition Nutrition 0.000 description 13
- 230000035764 nutrition Effects 0.000 description 12
- 239000000835 fiber Substances 0.000 description 10
- 230000002538 fungal Effects 0.000 description 8
- 239000012528 membrane Substances 0.000 description 8
- 210000004215 spores Anatomy 0.000 description 8
- 230000000844 anti-bacterial Effects 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 5
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- 238000004500 asepsis Methods 0.000 description 4
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- 239000000463 material Substances 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000011030 bottleneck Methods 0.000 description 3
- 238000001784 detoxification Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000011068 load Methods 0.000 description 3
- 230000001717 pathogenic Effects 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 229960001212 BACTERIAL VACCINES Drugs 0.000 description 2
- 244000052616 bacterial pathogens Species 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000001488 breeding Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 238000005755 formation reaction Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
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- 241000196324 Embryophyta Species 0.000 description 1
- 241001537207 Flammulina Species 0.000 description 1
- 210000000554 Iris Anatomy 0.000 description 1
- 235000007685 Pleurotus columbinus Nutrition 0.000 description 1
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- 238000000280 densification Methods 0.000 description 1
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Abstract
This utility model provides a kind of vaccination mechanism for cultivating edible fungi and strain container, belong to the cultivation apparatus field of edible fungi, this vaccination mechanism includes inoculation portion and cultivation portion, inoculation portion is provided with inoculation upper end open, cultivation portion is provided with cultivation lower ending opening, inoculation portion and cultivation portion connect, and are provided with the filter body without nutrient between inoculation portion and cultivation portion.Particulates emission cannot lead to filtering bodies to culture medium, and mycelia can be led to filtering bodies and be extended to culture medium and cultivate.This vaccination mechanism improves production efficiency, reduces technical threshold.Additionally, the strain container that this utility model provides includes bottle and above-mentioned vaccination mechanism, bottle is connected with cultivation portion.Utilizing this strain container real quickly to inoculate now with under collarium border, success rate is high.
Description
Technical field
This utility model is involved in the cultivation apparatus field of edible fungi, is used for cultivating edible fungi in particular to one
Vaccination mechanism and strain container.
Background technology
The cultivation of existing edible fungi needs just to carry out aseptic culture, sterile cultivating apparatus under conditions of rigorous aseptic
Putting into big, technology requirement height, complex operation, inefficiency, production cost is high high with technical threshold.
Utility model content
The purpose of this utility model is to provide a kind of vaccination mechanism for cultivating edible fungi, and overcoming can only be strictly
The aseptic culture that just can complete under aseptic condition, substantially increases production efficiency, reduces production cost and technical threshold.
Another object of the present utility model is to provide a kind of strain container for cultivating edible fungi, utilizes this strain to hold
Edible fungi cultivated by device, can substantially increase production efficiency, reduce production cost and technical threshold.
Embodiment of the present utility model is achieved in that
A kind of vaccination mechanism for cultivating edible fungi, is suitable to be connected, including inoculation portion, cultivation with the container of dress culture medium
Portion and the filter body without nutrient, inoculation portion is provided with inoculation upper end open, and cultivation portion is provided with cultivation lower ending opening, inoculation portion and
Cultivation portion connects, and cultivation portion is connected with container away from the one end in inoculation portion, and filter body is arranged between inoculation portion and cultivation portion.
A kind of strain container for cultivating edible fungi, it includes bottle and for cultivating the vaccination mechanism of edible fungi, bottle
The internal culture medium being provided with edible bacteria growing, bottle is connected with cultivation portion.
What this utility model embodiment provided provides the benefit that for the vaccination mechanism and strain container cultivating edible fungi:
By the inoculation upper end open in inoculation portion by edible bacterial vaccine inoculation to inoculation portion, owing to filtering the filtration of body, You Haiwei
Grain cannot pass through, and filter body can not provide the material base that pathogenic bacteria relies growth simultaneously, so particulates emission both cannot directly lead to
Filtering bodies, also cannot penetrate filter body to culture medium to sprout mode as mycelia, and the nutrition that mycelia can utilize himself is entered
Line splitting growth also extends to culture medium, and mycelia can be led to filtering bodies and be entered the culture medium that cultivation portion connects, and absorbs culture medium
Nutrition, carry out follow-up cultivation.The ingehious design of this vaccination mechanism, enormously simplify the seeded process of edible fungi, overcomes
The aseptic culture that just can only complete under stringent asepsis requirements, it is achieved have bacterium to inoculate, and success rate is high, substantially increases production
Efficiency, reduces production cost and technical threshold.Meanwhile, the strain container that this utility model embodiment provides, it passes through bottle
It is connected with vaccination mechanism, it is simple to the culture medium in mycelia absorption bottle body, utilizes this strain container energy real now with fast under collarium border
Speed inoculation, and success rate is high.
Accompanying drawing explanation
In order to be illustrated more clearly that the technical scheme of this utility model embodiment, will use required in embodiment below
Accompanying drawing be briefly described, it will be appreciated that the following drawings illustrate only some embodiment of the present utility model, the most should be by
Regard the restriction to scope as, for those of ordinary skill in the art, on the premise of not paying creative work, also may be used
To obtain other relevant accompanying drawings according to these accompanying drawings.
Fig. 1 provides the structural representation being used for cultivating the vaccination mechanism of edible fungi for this utility model first embodiment；
Fig. 2 provides the structural representation being used for cultivating the strain container of edible fungi for this utility model the second embodiment.
In figure:
Vaccination mechanism 100；
Inoculation portion 110；Inoculation upper end open 111；Inoculation lower ending opening 112；Inoculation dish 113；Through hole 114；Moisture film
115；
Cultivation portion 120；Cultivate upper end open 121；Cultivate lower ending opening 122；
Filter body 130；
Strain container 200；
Bottle 210.
Detailed description of the invention
For making the purpose of this utility model embodiment, technical scheme and advantage clearer, new below in conjunction with this practicality
Accompanying drawing in type embodiment, is clearly and completely described the technical scheme in this utility model embodiment, it is clear that retouched
The embodiment stated is a part of embodiment of this utility model rather than whole embodiments.Generally described in the accompanying drawing herein and
The assembly of this utility model embodiment illustrated can be arranged with various different configurations and design.
Therefore, below the detailed description of the embodiment of the present utility model provided in the accompanying drawings is not intended to limit requirement
The scope of the present utility model of protection, but it is merely representative of selected embodiment of the present utility model.Based in this utility model
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under not making creative work premise, all
Belong to the scope of this utility model protection.
It should also be noted that similar label and letter represent similar terms, therefore, the most a certain Xiang Yi in following accompanying drawing
Individual accompanying drawing is defined, then need not it be defined further and explains in accompanying drawing subsequently.
In description of the present utility model, it should be noted that term " on ", D score, "left", "right", " length ", " wide
Degree " etc. the orientation of instruction or position relationship be based on orientation shown in the drawings or position relationship, or this utility model product
The orientation usually put during use or position relationship, or the orientation that usually understands of those skilled in the art or position relationship,
It is for only for ease of description this utility model and simplifies description rather than instruction or imply that the device of indication or element must have
Specific orientation, with specific azimuth configuration and operation, therefore it is not intended that to restriction of the present utility model.Additionally, term
" first ", " second " etc. are only used for distinguishing and describe, and it is not intended that indicate or hint relative importance.
In description of the present utility model, in addition it is also necessary to explanation, unless otherwise clearly defined and limited, term " sets
Put " and " connection " should be interpreted broadly, connect for example, it may be fixing, it is also possible to be to removably connect, or be integrally connected；
Can be to be mechanically connected, it is also possible to be electrical connection；Can be to be joined directly together, it is also possible to be indirectly connected to by intermediary, permissible
It it is the connection of two element internals.For the ordinary skill in the art, can understand that above-mentioned term exists with concrete condition
Concrete meaning in this utility model.
First embodiment
Refer to Fig. 1, present embodiments provide a kind of vaccination mechanism 100 for cultivating edible fungi, this vaccination mechanism 100
Can be connected with container, it is simple to inoculate edible fungi and cultivate, container content has the culture medium of edible bacteria growing, and this connects
Planting mechanism 100 and include inoculation portion 110, cultivation portion 120 and filter body 130, inoculation portion 110 and cultivation portion 120 connect, cultivation portion 120
One end away from inoculation portion 110 is connected with container, and filter body 130 is arranged between inoculation portion 110 and cultivation portion 120.
Inoculation portion 110 is provided with inoculation upper end open 111 and inoculation lower ending opening 112, and inoculation upper end open 111 is loudspeaker
Shape opening, its internal diameter is more than the internal diameter of inoculation lower ending opening 112.The internal diameter of inoculation upper end open 111 is without being strict with, and it is high
Degree is maintained at more than 20mm.
Being provided with inoculation dish 113 in the present embodiment in inoculation portion 110, inoculation dish 113 is arranged on the top of filter body 130,
When being inoculated to inoculation portion 110 by inoculation upper end open 111, strain is inoculated on inoculation dish 113, in the present embodiment, inoculation
Being provided with multiple through hole 114 on dish 113, the mycelia of edible fungi wears filtering bodies 130 by this through hole 114, thus absorbs culture medium
Interior nutrition, and fungal spore and antibacterial can not lead to filtering bodies 130, fungal spore and antibacterial are completely cut off in training by logical filtering bodies 130
Support outside base, it is achieved that have the aseptic culture under bacterium state, the method greatly simplifiing inoculation, improve production effect simultaneously
Rate, reduces production cost and technical threshold.
Specifically, in the present embodiment a diameter of 2～4mm of the through hole 114 on inoculation dish 113, when harmful bacteria microgranule enters
By gravity behind fibrous airstream stagnant area, electrostatic adsorption is stopped by fiber, and edible fungi can rely on own growth to penetrate filter body
To culture medium, when the diameter of through hole 114 is more than 4mm, leakiness strain, and the strain pressure force of inoculation dish 113 is made to conduct to filtering body
130 make it deform, and come off, lose filtration.The through hole 114 distance apart from the edge of inoculation dish 113 need to be more than 3mm, and this sets
Meter can play certain cushioning effect, it is to avoid strain when inoculation dish 113 or inoculation portion 110 deform under the conditions of external force
With inoculation portion 110 or the sidewall contact of inoculation dish 113, can also prevent leakage from carrying disease germs air simultaneously.Certainly, in other embodiments
In, the number of the through hole 114 on inoculation dish 113 can be depending on the size of vaccination mechanism 100 or inoculation dish 113.
The present embodiment is filtered body 130 and is positioned at the lower section of inoculation dish 113, and the thickness of filter body 130 is 4～5mm, 4～5mm
Thickness can effectively filter out particulates emission, and strain is easy to logical filtering bodies 130 and is stretched into culture medium simultaneously；Filter body 130 is interior simultaneously
Footpath 1mm bigger than the internal diameter of inoculation dish 113, owing to filter body 130 elasticity is relatively big, beneficially clamping inoculation dish 113, and by inoculation dish 113
Edge seal.The distance of the bottom surface distance inoculation lower ending opening 112 of filter body 130 is 5～8mm.
In the present embodiment, inoculation portion 110 is provided with moisture film 115, and this moisture film 115 is mainly used in covering tightly inoculation upper end and opens
Mouth 111, rather than positive confinement, to ensure the humidity of kind of block.
Cultivation portion 120 is provided with cultivation upper end open 121 and cultivates lower ending opening 122, wherein, cultivates upper end open 121
It is connected with inoculation lower ending opening 112, cultivates lower ending opening 122 and be connected with culture medium.
The height in cultivation portion 120 is 20～23mm, and wherein cultivation portion 120 is near cultivating the part of upper end open 121 and connecing
Plant lower ending opening 112 to connect, and the height connected is 5～8mm, be i.e. set in cultivation upper end open when inoculation lower ending opening 112
121 outside time, cultivate upper end open 121 along cultivate upper end open 121 to cultivate lower ending opening 122 direction on 5～
The part of 8cm, is 5～8mm the most corresponding with the distance of the bottom surface distance inoculation lower ending opening 112 of filter body 130, i.e. cultivates upper end
The edge of opening 121 props up the bottom surface of filter body 130.
Cultivation portion 120 is near cultivating the part of lower ending opening 122 for being connected with culture medium, it is simple to strain leads to filtering bodies
130 enter lower space, eventually enter into culture medium, absorb the nutrition in culture medium.Owing to the energy for growth of mycelia can be along with life
The increase of distance and weaken, i.e. the length of mycelia is the shortest more good, the longest can injure mycelia, causes mycelia vitality to decline.This
In embodiment, it is preferable that after inoculation portion 110 is connected with cultivation portion 120, filter body 130 distance from bottom cultivate lower ending opening 122 away from
From for 15mm.
The distance of growth includes that the thickness filtering body 130 and filter body 130 arrive the distance cultivating lower ending opening 122, i.e. bacterium
The required growth distance planted is 19～20mm, now can guarantee that mycelia preferably energy for growth.
In the present embodiment, the internal diameter in cultivation portion 120 along cultivate upper end open 121 to cultivate the direction of lower ending opening 122 by
Cumulative the internal diameter making cultivation lower ending opening 122 is big greatly, and the power that can bear also becomes big, it is simple to keep the flat of cultivation portion 120
Weighing apparatus；The internal diameter simultaneously cultivating lower ending opening 122 is big, in " eight " font opening, can effectively prevent cultivating lower ending opening 122 and be absorbed in training
Support in base, simultaneously work as supporting role, it is ensured that culture medium separates with filter body 130.
Filter body 130 is to use geotextiles to make, it is preferred to use 600～800g/m2Geotextiles, preferred plan for use
800g/m2Geotextiles make in this utility model embodiment provide filter body 130.Owing to geotextiles has good permeable
Gas, without nutrient substance on this filter body 130, its microbial resistance is good, and owing to filtering the filtration of body 130, particulates emission cannot
Passing through, filter body 130 can not provide the material base that pathogenic bacteria relies growth simultaneously, so particulates emission both cannot be directly by filter
Body 130, also cannot penetrate filter body 130 to culture medium to sprout the mode as mycelia.But owing to the mycelia of edible fungi can pass through
The nutritional labeling of himself grows, and extends to the direction in cultivation portion 120, the length that mycelia generally can grow
Spending longer, under growth conditions appropriate state, most of kind kind truffles silks can be climbed wall and be grown more than 2 centimetres, but mycelia
Growing the longest, its mycelia vitality is the lowest, is more unfavorable for the cultivation of edible fungi, and the filter body 130 in the present embodiment is to cultivation portion 120
Distance between the culture medium connected is 19～20mm, and it is much smaller than the growth length of mycelia, the present embodiment sets 19～
20mm distance does not affect mycelia normal growth to culture medium, and absorbs the nutrition in culture medium, grows for it.
Referring to Fig. 1, inoculation portion 110 and cultivation portion 120 can be an entirety, it is also possible to for Split type structure, this enforcement
In example, preferably inoculation portion 110 and cultivation portion 120 are Split type structure, and inoculate lower ending opening 112 can be set in cultivation upper end
The outside of opening 121, forms the form of a sheathed clamping, by being set in cultivation portion 120 in inoculation portion 110, makes filter body
130 form the space of a relative closure to cultivation portion 120, owing to filter body 130 has hole, it is possible to permeable and ventilative,
Meet the oxygen needed for the growth of edible fungi.
Concrete, in the present embodiment, the internal diameter of the inoculation upper end open 111 in inoculation portion 110 is 40mm, inoculates lower ending opening
The internal diameter of 112 is 32mm, and the internal diameter filtering body 130 is also 32mm, and the internal diameter of the cultivation upper end open 121 in cultivation portion 120 is
31.6mm, the internal diameter cultivating lower ending opening 122 is 40mm, and it is big that inoculation portion 110 forms inoculation upper end open 111, and inoculation lower end is opened
Mouth 112 little horn cylindricals, and cultivation portion 120 formation cultivation upper end open 121 is little, cultivates the loudspeaker that lower ending opening 122 is big
The cylinder of shape.The stability cultivating lower ending opening 122 ambassador's cultivation portion 120 in cultivation portion 120 is more preferable.Certainly, real at other
Execute in example, inoculation portion 110, filter body 130 and cultivation portion 120 internal diameter size can according to customer demand, produce kind and
Many the considering such as cost selects, and is not limited in the present embodiment the size range provided.Vaccination mechanism 100 is permissible
Upper shield for container, it is also possible to for the lid of container, it is also possible to be other various structures.Preferred vaccination mechanism in the present embodiment
100 is the upper shield of container, by being set on container, thus realizes the connection with container.
Referring to Fig. 1, the principle of the vaccination mechanism 100 for cultivating edible fungi that the present embodiment provides is:
Carrying out the inoculation under open state in inoculation portion 110, the strain of edible fungi falls on inoculation dish 113, and leads to
Cross the through hole 114 on inoculation dish 113 and enter filter body 130, owing to fungal spore, antibacterial can be risen by the structure of filter body 130 densification
To the effect of filtration sterilization, and filtering body 130 and cannot provide the nutrition needed for fungal spore, bacterial growth, filter body 130 is also simultaneously
Nutrition can not be absorbed, so fungal spore and antibacterial cannot grow on filter body 130 in filter body 130 below cultivation portion 120
Breeding, wears filtering bodies 130 to sprout mode as mycelia, and for the mycelia of edible fungi, its can utilize its from
The nutrient substance of body grows, and towards the direction extension of culture medium, mycelia can absorb in culture medium easily
Nutrition, and by draw its follow-up growth of nutrition supplying.It is achieved thereby that had bacterium state to aseptic condition by open
Growth, the present embodiment overcomes the aseptic culture that just can only complete under stringent asepsis requirements, substantially increase production effect
Rate, reduces production cost and technical threshold.
Wherein, the mechanism of filter body 130 filtration sterilization is:
Inertial collision: owing to filter body 130 is made up of staggered fiber, staggered fiber constantly changes direction, bypass gas flow
Time, rubbed adhesion owing to microgranule inertia hits fiber；
Intercepting and be detained: when air velocity is down to stagnant area under critical, microgranule is slowly near being trapped by fiber；
Gravitational settling: gravity is more than sedimentation absorption during other external force such as air-flow；
Electrostatic Absorption: dry air produces electric charge and the synthetic fibers charges of different polarity with microgranule friction, attracts coupling, wherein, micro-
Biomone is many with electric charge.
After inoculating strain in inoculation portion 110, owing to inoculating under open environment, so inoculation portion 110 is contained within part
Particulates emission, such as: fungal spore and antibacterial.When particulates emission is more than 0.3um, mainly do inertia motion, and when harmful micro-
When grain is less than 0.1um, mainly doing Brownian movement, from point of theory, particulates emission (fungal spore and antibacterial) is 0.5～10um,
It is substantially carried out inertia motion, so Brownian movement is not discussed by this utility model embodiment.
Particulates emission is when together moving with air-flow, and air-flow constantly changes direction in staggered fiber, particulates emission due to
Inertial collision hits fiber, and particulates emission can be by filter body 130 friction clamping；Simultaneously after air-flow enters fiber retention district, microgranule
Slowly it is captured, due also to particulates emission is by electrostatic and action of gravity, and by fibers adsorption near fiber.And hypha of edible fungus depends on
By merisis, utilizing the nutrient of himself the most forwards to extend, hypha of edible fungus can overcome gravity with quiet
Electrical forces by filtering bodies 130 until culture medium.It is achieved thereby that had the growth to aseptic condition of the bacterium state by open,
The present embodiment overcomes the aseptic culture that just can only complete under stringent asepsis requirements, substantially increases production efficiency, reduces
Production cost and technical threshold.
The present embodiment, by providing vaccination mechanism 100 that edible fungi is carried out Open-Architecture Controller, in seeded process, is not required to
To inoculate in the environment of rigorous aseptic, simultaneously in follow-up incubation, to which avoids Xie Kou, tying or upper cover etc.
Operation, it is artificial eye-liquid inoculation speed 2 in prior art that the vaccination mechanism 100 using the present embodiment to provide carries out artificial vaccination
Times, it is in prior art 40 times of artificial solid vaccination speed, and has higher success rate.
Compared in prior art, the aseptic culture that just can need to complete at strict aseptic condition, its equipment investment is big, skill
Art requires height, complex operation, inefficiency；And the present embodiment realizes there is the aseptic culture under bacterium state, substantially increase production
Efficiency, reduces production cost and technical threshold.And output and quality penetration and promotion high, easy, and can whole year production.
In the present embodiment, inoculation portion 110 and cultivation portion 120 are tubular, and filter body 130 is circular tabular, and at other
In embodiment, inoculation portion 110, cultivation portion 120 and filter body 130 can be square or other are variously-shaped, and its size also can root
It is adjusted according to concrete condition.
Repeatedly proving through the fact, this utility model success rate (occurs under sterilizing room duty far above sterilizing room inoculation
Amount be 500～1000/people/minute), its average pollution rate is 5～10%, and this utility model there is not yet pollution.
It addition, this utility model and other modes of production have the detoxification function that can not possess, mycelia is empty by filtering below body 130
Between grow to culture medium.
In a word, this technique can be greatly improved inoculation efficiency, and it is 2 times of production technology liquid inoculation speed, and industrialization is solid
Body inoculates 40 times, and this utility model need not open bottleneck or bag mouth, fastens bottleneck or bag mouth, and activity is not by any restriction, no
With set glove, aseptic take work.
Investment in fixed assets is almost without comparability, and except having filter body and moisture film part, conversion is to every bag of meter, relative on an equal basis
For solid Hand Inoculations, only need to inoculate speed and promote 10% i.e. recoverable cost.
This utility model cost is extremely low, ultrahigh in efficiency, and yield and quality is excellent, and may replace fruiting ring, has detoxification concurrently, especially
It is beneficial to penetration and promotion without technical threshold, pole, and it has thoroughly been dissolved medium and high classes mushroom grog production bottleneck and (has inoculated careful, numerous
Trivial, easy infection, efficiency are low) etc. a difficult problem, simultaneously its can directly be transported to send out bacterium room inoculation, eliminate the operation carried back and forth, meaning
Huge.
Second embodiment
Referring to Fig. 1 and Fig. 2, provide a kind of strain container 200 for cultivating edible fungi in the present embodiment, it includes
The vaccination mechanism 100 for cultivating edible fungi that bottle 210 and this utility model first embodiment provide, is arranged in bottle 210
The culture medium of edible bacteria growing, bottle 210 is had to be connected with cultivation portion 120.
By vaccination mechanism 100 is connected formation strain container 200 with bottle 210, available strain container 200 is the most right
Edible fungi is cultivated, more convenient to operate.Depending on the shapes and sizes of bottle 210 can be with situation simultaneously, this bottle 210 is permissible
For various types of bottles 210, it is also possible to for bag, by loading culture medium in bag, then by bag and vaccination mechanism
100 connect, and also can form strain container 200.
It is connected with vaccination mechanism 100 by bottle 210, it is simple to mycelia absorbs the nutrient substance of the culture medium in bottle 210,
Utilize this strain container 200 real quickly to inoculate now with under collarium border, and success rate is high.Strain container 200 can simplify training
Educate the operation of edible fungi, make inoculation be easier to, the quick inoculation in collarium border can be realized, substantially increase production efficiency, reduce
Production cost and technical threshold.
Referring to Fig. 1 and Fig. 2, provide a kind of method for cultivating edible fungi in the present embodiment, it includes following step
Rapid:
S1, prepare strain container 200.
Loading culture medium in bottle 210, the vaccination mechanism 100 then this utility model first embodiment provided is sheathed
On bottle 210, make the strain container 200 that this utility model the second embodiment provides, and to strain container 200 sterilizing.
Specifically, this utility model is selected edible mushroom cultivating bag as bottle 210, in edible mushroom cultivating bag, add training
Supporting base, be then connected with edible mushroom cultivating bag in the cultivation portion 120 of vaccination mechanism 100, wherein cultivation portion 120 is hollow cylinder
Shape, and its cultivation lower ending opening 122 is relatively big, edible mushroom cultivating bag stretches in cultivation portion 120 by cultivating lower ending opening 122, and
Through cultivating upper end open 121, then edible mushroom cultivating bag is outwardly and downwardly turned down, expose the inner passage in cultivation portion 120.
Then the inoculation portion 110 with filter body 130 and inoculation dish 113 is set in the outside in cultivation portion 120, i.e. makes strain container
200, then this strain container 200 is carried out sterilizing.Wherein, the selection of culture medium and the concrete operations of sterilizing can refer to existing skill
Art.
This utility model embodiment before sterilization, at the inoculation upper end open 111 upper cover lastblock damp-proof membrane in inoculation portion 110
(not shown), is then fixed on damp-proof membrane in inoculation portion 110 with rubber band, carries out sterilizing the most again, and this damp-proof membrane is prevented from
Filter body 130 makes moist, simultaneously when high moisture vapor sterilizing, moreover it is possible to reach the purpose of moisturizing.
When putting sterilizing owing to crouching, often have minority bacterium bag moisture distribution inequality and flow to filter body, cause infecting, for avoiding
The generation of this situation, sets up inner ring (not shown) to the position placing filter body 130 in inoculation portion 110, one end of inner ring and filter
Body 130 connects, and the other end is towards inoculation lower ending opening 112, and the effect of inner ring is possible of isolation filter body 130 and contact with moisture
Property.The height of inner ring is 8mm, and it is 4mm with the distance in the outside in inoculation portion 110.
Some kind needs to use heavy caliber fruiting to iris out mushroom, can use large-sized vaccination mechanism 100, this reality accordingly
Fruiting circle is can be used as with the cultivation portion 120 of novel offer, for preventing sleeping bacterium bag of putting from collapsing material and contact filter body 130 further, can be
Inner ring bottom puts a netted Ka Gai (figure does not regards).
S2, inoculation step.
By on edible fungi inoculation to inoculation portion 110, it is not necessary to inoculate in the environment of rigorous aseptic, simultaneously without solving
The routine operations such as mouth, tying or upper cover, only need to open damp-proof membrane, be then placed in kind of a block, and this inoculation step is simple, can be quickly
Inoculation, greatly substantially increases production efficiency, reduces production cost and technical threshold.
Use of the right age strain, it is not necessary to any form is sterilized.Natural environment.The most movable inoculation, it may be assumed that directly capture kind of a block with hands
Put in inoculation portion 110, preferably strain is filled up inoculation portion 110.
S3, membrane step.
After inoculation step completes, the upper surface at strain container 200 draws last layer moisture film 115.This moisture film 115 energy
Enough keep edible fungi desired moisture level in strain container 200, be conducive to accelerating the growth of edible fungi, if environment is the driest, can
Water wet to bag bottom.If needing to accelerate to send out bacterium, inoculation portion 110 and cultivation portion 120 can be loosened after mycelia covers with surface
Seam.
Specifically, this moisture film 115 can be independently arranged on strain container 200, is i.e. arranged on inoculation portion 110 near connecing
Planting the position of upper end open 111, this moisture film 115 after inoculation terminates in batch, can also draw a bigger moisturizing when producing
Film 115, it can cover multiple strain container 200.
In the present embodiment, preferably draw moisture film 115 by unified for multiple strain containers 200, time saving and energy saving, more save into simultaneously
This, can use the most common mulch film etc. as moisture film 115, and its low cost, same membrane is simple to operate.
Certainly, in other embodiments, this membrane step can also be omitted.
S4, incubation step.
After membrane step completes, can carry out the cultivation of routine, fruiting, this step refers to prior art.
Cultivate edible fungi by utilizing strain container 200 to carry out, simplify the breeding method of edible fungi, make the training of edible fungi
Educating simpler, operation is more prone to.This vaccination mechanism 100 is reusable repeatedly simultaneously, only need to change filter body every time,
With low cost.
In sum, the present embodiment by the inoculation upper end open 111 in inoculation portion 110 by edible bacterial vaccine inoculation to inoculation
In portion 110, strain eventually falls on filter body 130, and owing to filtering the filtration of body 130, particulates emission cannot pass through, and filters body simultaneously
130 can not provide the material base that pathogenic bacteria relies growth, so particulates emission both cannot directly lead to filtering bodies, and also cannot be to sprout
The mode for mycelia of sending out penetrates filter body 130 to culture medium, so when inoculation, it is not necessary to inoculate in an aseptic environment, environment is deposited
Fungal spore and antibacterial cannot lead to filtering bodies 130 on filter body 130, and mycelia can utilize the nutrition of himself to carry out point
Schizogenesis length also extends to culture medium, and mycelia can be led to filtering bodies 130 and be entered the culture medium that cultivation portion 120 connects, and absorbs cultivation
The nutrition of base, carries out follow-up cultivation.The ingehious design of this vaccination mechanism 100, enormously simplify the seeded process of edible fungi, gram
Take the aseptic culture that just can only complete under stringent asepsis requirements, it is achieved have bacterium to inoculate, and success rate is high, substantially increases
Production efficiency, reduces production cost and technical threshold.Meanwhile, strain container 200 passes through to load culture medium in bottle 210,
Then it is connected with vaccination mechanism 100, it is simple to mycelia absorbs the nutrition of the culture medium in bottle 210, utilizes this strain container 200 energy
Quickly inoculate now with under collarium border in fact, and success rate is high.Additionally, utilize strain container 200 to carry out cultivating edible fungi, simplify
The method cultivating edible fungi, makes inoculation be easier to, and can realize the quick inoculation in collarium border, substantially increase production efficiency, fall
Low production cost and technical threshold.
Compared with prior art, this utility model has the advantage that
(1) aseptic culture under the present embodiment achieves bacterium state.
(2) greatly improve production efficiency, reduce inoculation technique threshold, not by sterile working and environmental constraints.
(3) filter body 130 has detoxification, cultivation portion 120 can double as fruiting ring.
(4) can directly be transported to cultivation after completing sterilizing complete quickly to inoculate, it is not necessary to transfer room transit link back and forth.
(5) without sterilizing room and various sterilization with staticize equipment, cost puts into extremely low.
(6) reason mycelia penetrate filtering layer do not deposit to culture medium operation and inoculation gnotobasis carry disease germs cause pollute, success rate
Higher.
(7) sterile culture, yield and quality is the most excellent.
The vaccination mechanism that this utility model provides can be applicable to Pleurotus ostreatus, Pleurotus eryngii, Flammulina velutiper (Fr.) Sing etc..
The foregoing is only preferred embodiment of the present utility model, be not limited to this utility model, for this
For the technical staff in field, this utility model can have various modifications and variations.All in spirit of the present utility model and principle
Within, any modification, equivalent substitution and improvement etc. made, within should be included in protection domain of the present utility model.
Claims (10)
1., for cultivating a vaccination mechanism for edible fungi, be suitable to be connected with the container of dress culture medium, it is characterised in that include connecing
Kind portion, cultivation portion and the filter body without nutrient, described inoculation portion is provided with inoculation upper end open, and described cultivation portion is provided with cultivation
Lower ending opening, described inoculation portion and described cultivation portion connect, and described cultivation portion is away from the one end in described inoculation portion and described container
Connecting, described filter body is arranged between described inoculation portion and described cultivation portion.
Vaccination mechanism for cultivating edible fungi the most according to claim 1, it is characterised in that arrange in described inoculation portion
There are inoculation dish, described inoculation dish to be arranged on the top of described filter body, described inoculation dish is provided with multiple through hole.
Vaccination mechanism for cultivating edible fungi the most according to claim 1, it is characterised in that described inoculation portion is provided with
For covering tightly the moisture film of described inoculation upper end open.
Vaccination mechanism for cultivating edible fungi the most according to claim 1, it is characterised in that described inoculation portion and described
Cultivation portion is Split type structure, and described inoculation portion is additionally provided with inoculation lower ending opening, and described cultivation portion is additionally provided with cultivation upper end and opens
Mouthful, described inoculation lower ending opening is optionally set in the outside of described cultivation upper end open, described filter body be arranged on described in connect
Kind portion is near the position of described inoculation lower ending opening.
Vaccination mechanism for cultivating edible fungi the most according to claim 4, it is characterised in that described inoculation upper end open
For horn opening, the internal diameter of described inoculation upper end open is more than the internal diameter of described inoculation lower ending opening.
Vaccination mechanism for cultivating edible fungi the most according to claim 4, it is characterised in that the internal diameter in described cultivation portion
It is gradually increased along described cultivation upper end open to the direction of described cultivation lower ending opening.
Vaccination mechanism for cultivating edible fungi the most according to claim 4, it is characterised in that described filter body is near described
The side in cultivation portion is 15mm to the distance of described cultivation lower ending opening.
Vaccination mechanism for cultivating edible fungi the most according to claim 1, it is characterised in that described filter body is by geotextiles
Make.
9. one kind for cultivating the strain container of edible fungi, it is characterised in that it includes bottle and as arbitrary in claim 1～8
The vaccination mechanism for cultivating edible fungi described in Xiang, is provided with the culture medium of edible bacteria growing, described bottle in described bottle
Body is connected with described cultivation portion.
Strain container for cultivating edible fungi the most according to claim 9, it is characterised in that described bottle is edible
Bacteria cultivation bag.
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| CN201620579488.2U CN205681918U (en) | 2016-06-13 | 2016-06-13 | A kind of vaccination mechanism for cultivating edible fungi and strain container |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105875202A (en) * | 2016-06-13 | 2016-08-24 | 蔡志闯 | Inoculation mechanism, strain containers and method for culturing edible mushrooms |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105875202A (en) * | 2016-06-13 | 2016-08-24 | 蔡志闯 | Inoculation mechanism, strain containers and method for culturing edible mushrooms |
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