CN204265763U - Active somatic cell culture carrier - Google Patents
Active somatic cell culture carrier Download PDFInfo
- Publication number
- CN204265763U CN204265763U CN201420665155.2U CN201420665155U CN204265763U CN 204265763 U CN204265763 U CN 204265763U CN 201420665155 U CN201420665155 U CN 201420665155U CN 204265763 U CN204265763 U CN 204265763U
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- China
- Prior art keywords
- culture carrier
- pit
- somatic cell
- active somatic
- drop
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Abstract
The utility model provides a kind of active somatic cell culture carrier, described culture carrier is provided with multiple pit for carrying active somatic cell drop on the surface, and described culture carrier surface and pit surface are super hydrophobic surface, pit edge arranges closed hoop projection, and prominence has oil-wetted surface characteristic.When carrying out being fertilized and cultivating on such culture carrier, because cell drop (aqueous solution) is spherical in shape on the super hydrophobic surface of culture carrier, and the contact area on surface is very little, does not adhere in its surface.And the cultivation oil that the closed hoop projection restriction with oil-wetted surface characteristic is cultivated outside drop is excessive, ensure that cultivating drop is in independently culture environment all the time.Therefore, cell drop (aqueous solution) can really navigate on the position of required (fertilization and cultivation) by automatic precision under gravity environment, thus is beneficial to fertilization and cultivates operation.
Description
Technical field
The utility model relates to a kind of container of cell cultures, particularly relates to a kind of active somatic cell culture carrier.
Background technology
In the prior art, carry out the surface water-wetted surface often of fertilization and the culture vessel be fertilized and used in culturing process at ovum, like this, cell drop (aqueous solution) is semisphere on surface, is adhered fixed on surface.Therefore, when carrying out being fertilized and cultivating on such culture vessel, because cell drop (aqueous solution) can be adhered fixed the hydrophilic surface (optional position) at culture vessel, cause cannot naturally accurately navigating on the position of required (fertilization and cultivation), thus be unfavorable for automatization insemination and cultivate operation.
Utility model content
The purpose of this utility model is to provide a kind of active somatic cell culture carrier, effectively can realize cell drop (aqueous solution) automatic precision on culture carrier surface and determine position.
The utility model provides a kind of active somatic cell culture carrier, and described culture carrier is provided with multiple pit for carrying active somatic cell drop on the surface, and described pit surface is super hydrophobic surface.
As the improvement of such scheme, described pit surface is laid the super hydrophobic surface layer of one deck super hydrophobic material.
As the improvement of such scheme, described pit surface is the super hydrophobic surface through super-hydrophobic process.
As the improvement of technique scheme, described culture carrier on the surface described in each the edge protuberance of pit the restrictions of closed hoop is set, and the surface of described restrictions is oil-wetted surface.
As the improvement of such scheme, described pit surface is cambered surface.
As the improvement of such scheme, described pit surface is semicircle sphere.
As the improvement of such scheme, described active somatic cell culture carrier is single-row multiple-unit culture carrier, and the multiple described pit on described culture carrier surface is arranged in a linear.Compared with prior art, active somatic cell culture carrier disclosed in the utility model is by being provided with multiple pit for carrying active somatic cell drop on the surface at culture carrier, and described pit surface is the super hydrophobic surface through super-hydrophobic process, like this, when carrying out being fertilized and cultivating on such culture carrier, because cell drop (aqueous solution) is upper spherical in shape in the pit surface (super hydrophobic surface) of culture carrier, very little with the contact area on surface, do not adhere in its surface.In addition, because the pit edge projection on culture carrier surface arranges the restrictions of closed hoop, and this restrictions has oil-wetted surface characteristic, can cultivation oil outside restrictive cell drop excessive, ensure that cell drop is in independently in culture environment all the time.Drop is cultivated in super hydrophobic surface carrying, covers and cultivates oil, and this structure can ensure that nutrient solution drips off whole noresidue transfer.Because the proportion of cell is a bit larger tham nutrient solution, nutrient solution proportion is greater than cultivates oil, on super hydrophobic surface, cultivate drop and be substantially in suspended state, in gravity environment, drop is automatically positioned on the lower-most point on culture carrier surface, thus be beneficial to fertilization and cultivate operation, be conducive to the transfer of drop.
Accompanying drawing explanation
Fig. 1 is the schematic top plan view of the structure of a kind of active somatic cell culture carrier in the utility model embodiment.
Fig. 2 is the diagrammatic cross-section of the structure of a kind of active somatic cell culture carrier in the utility model embodiment.
Fig. 3 is the structure for amplifying schematic diagram of a kind of active somatic cell culture carrier in the utility model embodiment.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, be clearly and completely described the technical scheme in the utility model embodiment, obviously, described embodiment is only the utility model part embodiment, instead of whole embodiments.Based on the embodiment in the utility model, those of ordinary skill in the art are not making the every other embodiment obtained under creative work prerequisite, all belong to the scope of the utility model protection.
See Fig. 1 ~ 2, it is the structural representation of a kind of active somatic cell culture carrier that the utility model embodiment provides.This active somatic cell culture carrier 10 comprises culture carrier main body 11, and this culture carrier main body 11 is lumphy structure, is preferably square or rectangle.The surface 111 of described culture carrier main body 11 is arranged with multiple pit 12 downwards, and described pit 12 is for carrying active somatic cell drop.Wherein, the surface 111 of described culture carrier main body 11 and the surface 121 of pit 12 described in each are super hydrophobic surface.Such as, surface 111 and the surface 121 of pit 12 described in each of described culture carrier main body 11 are the super hydrophobic surface layer having laid one deck super hydrophobic material, or the surface 111 of described culture carrier main body 11 and the surface 121 of pit 12 described in each are the super hydrophobic surface through super-hydrophobic process.
On the surface 111 of described culture carrier main body 11, described in each, the edge protuberance of pit 12 arranges the restrictions 13 of closed hoop, and the surface of described restrictions 13 is oil-wetted surface.The cultivation oil that the protruding restrictions 13 of closed hoop with oil-wetted surface characteristic can limit outside active somatic cell drop is excessive, ensures that active somatic cell drop is in independently culture environment all the time.
Such as, as shown in Figure 3, when the culture carrier that described pit surface 121 is super hydrophobic surface carries out being fertilized and cultivating, cell drop (aqueous solution) 502 containing cell 501 is spherical in shape on the super hydrophobic surface of pit surface 121, very little with the contact area of pit surface 121, do not adhere to and (be equivalent to suspended state) in its surface, can really navigate in pit surface 121 (and being the lower-most point of pit surface 121) by automatic precision under gravity environment.And the edge protuberance of pit 12 arranges the restrictions 13 of closed hoop described in each, and the surface of described restrictions 13 is oil-wetted surface, can cultivation oil 503 (for covering pit surface 121 and being placed in the cell drop 502 in this pit surface 121) outside restrictive cell drop (aqueous solution) 502 excessive, thus ensure that cell drop (aqueous solution) 502 is in independently culture environment all the time.
Preferably, in the present embodiment, as shown in Figure 2, the surface 121 of pit 12 described in each is cambered surface, is preferably semicircle sphere.Described in each, pit 12 is in the same size, the degree of depth of pit 12 and width experimentally demand and arrange difference.
Preferably, in the present embodiment, multiple pits in described culture carrier main body 11 12 in single file linear arrangement on the surface 111 of described culture carrier main body 11, from but the culture carrier 1 formed is single-row multiple-unit culture carrier.
In sum, active somatic cell culture carrier disclosed in the utility model is by being provided with multiple pit 12 for carrying active somatic cell drop on the surface at culture carrier 1, and described pit surface 121 is super hydrophobic surface, like this, when carrying out being fertilized and cultivating on such culture carrier, because cell drop (aqueous solution) is spherical in shape on the super hydrophobic surface of culture carrier, and the contact area on surface is very little, does not adhere in its surface.In addition, because the pit edge projection on culture carrier surface arranges the restrictions of closed hoop, and this restrictions has oil-wetted surface characteristic, can cultivation oil outside restrictive cell drop excessive, ensure that cell drop is in independently in culture environment all the time.Drop is cultivated in super hydrophobic surface carrying, covers and cultivates oil, and this structure can ensure that nutrient solution drips off whole noresidue transfer.Because the proportion of cell is a bit larger tham nutrient solution, nutrient solution proportion is greater than cultivates oil, on super hydrophobic surface, cultivate drop and be substantially in suspended state, in gravity environment, drop is automatically positioned on the lower-most point on culture carrier surface, thus be beneficial to fertilization and cultivate operation, be conducive to the transfer of drop.
The above is preferred implementation of the present utility model; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the utility model principle; can also make some improvements and modifications, these improvements and modifications are also considered as protection domain of the present utility model.
Claims (7)
1. an active somatic cell culture carrier, is characterized in that, described culture carrier is provided with multiple pit for carrying active somatic cell drop on the surface, and described pit surface is super hydrophobic surface.
2. active somatic cell culture carrier as claimed in claim 1, it is characterized in that, described pit surface is laid the super hydrophobic surface layer of one deck super hydrophobic material.
3. active somatic cell culture carrier as claimed in claim 1, it is characterized in that, described pit surface is the super hydrophobic surface through super-hydrophobic process.
4. active somatic cell culture carrier as claimed in claim 1, is characterized in that, described culture carrier on the surface described in each the edge protuberance of pit the restrictions of closed hoop is set, and the surface of described restrictions is oil-wetted surface.
5. the active somatic cell culture carrier as described in any one of Claims 1 to 4, is characterized in that, described pit surface is cambered surface.
6. the active somatic cell culture carrier as described in any one of Claims 1 to 4, is characterized in that, described pit surface is semicircle sphere.
7. active somatic cell culture carrier as claimed in claim 1, it is characterized in that, described active somatic cell culture carrier is single-row multiple-unit culture carrier, and the multiple described pit on described culture carrier surface is arranged in a linear.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420665155.2U CN204265763U (en) | 2014-11-06 | 2014-11-06 | Active somatic cell culture carrier |
| PCT/CN2014/090998 WO2016070454A1 (en) | 2014-11-06 | 2014-11-13 | Automation device, carrier and method for oocyte in vitro fertilization and cleavage culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420665155.2U CN204265763U (en) | 2014-11-06 | 2014-11-06 | Active somatic cell culture carrier |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204265763U true CN204265763U (en) | 2015-04-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420665155.2U Expired - Fee Related CN204265763U (en) | 2014-11-06 | 2014-11-06 | Active somatic cell culture carrier |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204265763U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017000419A1 (en) * | 2015-06-30 | 2017-01-05 | 徐小杨 | Cell droplet rapid vitrification freeze-thawing carrier and method |
| WO2022000641A1 (en) * | 2020-07-03 | 2022-01-06 | 清华大学 | Super-hydrophobic micro-pit array chip, and production method therefor and device thereof |
-
2014
- 2014-11-06 CN CN201420665155.2U patent/CN204265763U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017000419A1 (en) * | 2015-06-30 | 2017-01-05 | 徐小杨 | Cell droplet rapid vitrification freeze-thawing carrier and method |
| WO2022000641A1 (en) * | 2020-07-03 | 2022-01-06 | 清华大学 | Super-hydrophobic micro-pit array chip, and production method therefor and device thereof |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160930 Address after: 510320, Guangdong Guangzhou International Biological Island spiral four Road No. 1 standard industrial unit, the first phase of the development of B District 404 units Patentee after: GUANGZHOU LANRI BIOTECHNOLOGY CO.,LTD. Address before: 510550, room 183, 305 middle Yuexiu Road, Yuexiu District, Guangdong, Guangzhou Patentee before: Xu Xiaoyang |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150415 Termination date: 20211106 |
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| CF01 | Termination of patent right due to non-payment of annual fee |