CN103620018B - Cell cultivator - Google Patents
Cell cultivator Download PDFInfo
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- CN103620018B CN103620018B CN201180071316.4A CN201180071316A CN103620018B CN 103620018 B CN103620018 B CN 103620018B CN 201180071316 A CN201180071316 A CN 201180071316A CN 103620018 B CN103620018 B CN 103620018B
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- cell
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
- C12M25/04—Membranes; Filters in combination with well or multiwell plates, i.e. culture inserts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Provided is a cell cultivator, said cell cultivator includes a hollow tank, a removable membrane, a fixed sheath and a support seat, wherein the removable membrane is fixed on the bottom opening of the tank, the support seat is located on the outside of the tank and supports the tank, living cells or others biological materials can be placed on the membrane to culture. The position of the tank of said cell cultivator, relative to the support seat, can be adjusted to facilitate the microscope focusing. After the cell culture is ending, the fixed sheath can be removed from the bottom of the tank in order to remove the membrane to observe it or to use it in clinical and medical applications.
Description
Technical field
The invention relates to a kind of cell culture apparatus, espespecially a kind of cell cultures utensil that can be applicable to active somatic cell field of cultivating and use, or as the carrier that biomaterial microscopic examination field uses, so that active somatic cell or other biological material carry out the cell culture apparatus design of microscopic examination and shooting record.And this cell culture apparatus also to can be applicable to cultivate after active somatic cell by it indirectly or directly transplanting human body or animal body, or further in any form in conjunction with biomedical material in the medium Application Areas of cell training device.
Background technology
As in the correlative technology field such as research and apply of cytobiology, for carrying out the cultivation of cell in vitro or shaping, cell strain is set up in order to organize, the test of cell physiological toxicity, cell migration are observed, various research and apply between cell and another cell or other extracellular matrix or the factor, or when experiment made on the living is difficult to walk, cell culture apparatus usually must be used to provide the purposes of stem cell and other all kinds cell injuring model.Above-mentioned various cell can be combined with any type of biomedical material further, and is applied to medical research and graft application.
Current existing cell culture apparatus design, it mainly comprises cell body and a diaphragm that one has three feets, and form the accommodation space of a up/down perforation shape in described cell body, this diaphragm is fixed on cell body bottom opening place.When this cell culture apparatus is applied to cell cultures, be that the diaphragm be fixed on bottom cell body provides active somatic cell to adhere to its surface, and leaching is placed in substratum, and active somatic cell can be survived growth.
In aforesaid cell culture apparatus, mostly cell body is to be shaped with forging type manufacture by mold, though cell body takes the tectonic sieving of foot-rest type, can provide and cultivate based on circulation good on diaphragm, grow up in order to active somatic cell, but, because the cell body using moulding manufacture is that a shape is fixed, immutable type body, not there is adjustable, and diaphragm is fixed on bottom cell body and cannot tears arbitrarily group open, when applying in clinical treatment, or user for carrying out viable cell observation or filming image time, micro-imaging observation must be carried out together with cell body, time wherein because of use high power objective, its high power camera lens NA value is large, operating distance is short, when lens focusing adjusts, easily because cell body is positioned at the obstruction above diaphragm, and unfavorable microscope obtains image more clearly to diaphragm.
Summary of the invention
The technical problem to be solved in the present invention is: the diaphragm improving current existing cell culture apparatus is fixed on the shortcoming being not easy to bottom cell body disassemble.
For solving aforesaid technical problem, technical solution of the present invention is: provide a kind of cell culture apparatus, it comprises:
The cell body of one hollow form, a upper shed is formed on cell body top, forms the location division that an opening and is positioned at this mouth periphery bottom cell body;
One diaphragm be the opening part be covered in bottom cell body of removable group, and diaphragm periphery extends to the side, location division bottom cell body;
One permanent sleeve, on the location division being installed in bottom cell body of removable group, is fixed on by diaphragm bottom cell body; And
One supporting seat, be comprise a pedestal, pedestal is mounted on outside cell body and supports cell body.
In cell culture apparatus as above, the pedestal of this supporting seat has an annular solid, props up feet in annular base body root edge to downward-extension number, and this pedestal can adjust up and down and positioningly be mounted on outside cell body.
In cell culture apparatus as above, this pedestal top forms the inner ring protuberance diametrically extended inwardly, inner ring periphery formation in inner ring protuberance is spaced formed adjustment location division by multiple convex grain, the periphery of this cell body above location division forms several recessed edge in upper and lower spaced location, there is provided clip location, adjustment location division wherein, make cell body energy lift adjustment height.
In cell culture apparatus as above, bottom this pedestal inner ring protuberance, form the pressure section that can push against permanent sleeve.
In cell culture apparatus as above, this location division is the ring-type Access Division forming in cell body bottom periphery face, this permanent sleeve has the ring-type body that is sheathed on cell body periphery, this ring-type body inner peripheral surface forms ring-type Fixed Division, ring-type Access Division and ring-type Fixed Division are the male-female engagement forming arc surface, make that permanent sleeve is sheathed is fixed on cell body periphery, diaphragm is gripped between ring-type Access Division and ring-type Fixed Division.
In cell culture apparatus as above, ring-type Access Division is the arc surface flange of ring-type, and ring-type Fixed Division is the recessed edge of arc surface of ring-type.
In cell culture apparatus as above, ring-type Access Division is the recessed edge of arc surface of ring-type, and ring-type Fixed Division is the arc surface flange of ring-type.
In cell culture apparatus as above, the ring-type body bottom of this permanent sleeve forms the ring root edge extended inwardly, and the ring root edge of permanent sleeve is relative with the location division of cell body bottom clamps diaphragm.
In cell culture apparatus as above, ring-type Access Division is the arc surface flange of ring-type, and ring-type Fixed Division is the recessed edge of arc surface of ring-type; The ring-type body bottom of this permanent sleeve forms the ring root edge extended inwardly, and the ring root edge of permanent sleeve is relative with the location division of cell body bottom clamps diaphragm.
In cell culture apparatus as above, ring-type Access Division is the recessed edge of arc surface of ring-type, and ring-type Fixed Division is the arc surface flange of ring-type; The ring-type body bottom of this permanent sleeve forms the ring root edge extended inwardly, and the ring root edge of permanent sleeve is relative with the location division of cell body bottom clamps diaphragm.
Advantage of the present invention is: in cell cultivation process, user is except the suitable height location that the diaphragm leaching being located at cell body bottom surface can be utilized to be placed in the substratum of incubator, the nutrient solution allowing substratum comprise and additive have good permeability in diaphragm, make the active somatic cell of membrane surface can be subject to outside good cultivation; When user is for carrying out cell observation by microscope, annular base body is up adjusted, to make bottom cell body can close to or be placed on microscopical Stage microscope, allow user can the focusing of accurate adjustment microscope, to obtain comparatively ideal micro-imaging.Come to an end in cell cultures, also permanent sleeve can be pulled down from cell body bottom surface, carry out cell or the transplanting of celliferous biomedical material, or take out diaphragm be placed in microscopical object lens under directly observe, so, the supporting seat of cell body and periphery thereof can be avoided microscopical obstruction, allow user focus by accurate adjustment microscope, and obtain comparatively ideal cell image.
In cell culture apparatus of the present invention, the pedestal of this supporting seat still can be utilized to have an annular solid, prop up feet in annular base body root edge to downward-extension number, this pedestal can adjust up and down and positioningly be mounted on outside cell body, is easy to adjust its height location up and down in order to the diaphragm be arranged at bottom cell body.
Accompanying drawing explanation
Fig. 1 is the perspective exploded view of the first preferred embodiment of cell culture apparatus of the present invention.
Fig. 2 is the combined planar schematic diagram of the preferred embodiment of cell culture apparatus first shown in Fig. 1.
Fig. 3 is the close-up schematic view of the preferred embodiment of cell culture apparatus first shown in Fig. 2.
Fig. 4 be another preferred embodiment of exchanging of the ring-type Access Division of the preferred embodiment of cell culture apparatus shown in Fig. 1 and the concaveconvex shape of ring-type Fixed Division combined planar schematic diagram.
Fig. 5 is the combined planar schematic diagram of cell culture apparatus second of the present invention preferred embodiment.
Fig. 6 is the close-up schematic view of the preferred embodiment of cell culture apparatus second shown in Fig. 5.
Fig. 7 is that the supporting seat in the preferred embodiment of cell culture apparatus first shown in Fig. 1 rises and reduces the floor map of cell culture apparatus height.
Fig. 8 is the operation chart that the preferred embodiment of cell culture apparatus first shown in Fig. 1 is disassembled.
Description of reference numerals
10-cell body; 11-opening; 12-location division; 13,13a-ring-type Access Division; 14-locates recessed edge; 15-ring top flange; 20-diaphragm; 30-permanent sleeve; 300-ring-type body; 301-ring root edge; 31,31a-ring-type Fixed Division; 40-supporting seat; 41-pedestal; 42-feet; 43-inner ring protuberance; 44-adjusts location division; The convex grain of 441-; 45-pressure section.
Embodiment
Below coordinating preferred embodiment graphic and of the present invention, setting forth the technique means that the present invention takes for reaching predetermined goal of the invention further.
As shown in Figure 1 to Figure 4 with shown in Fig. 5 to Fig. 6, several preferred embodiments disclosing cell culture apparatus of the present invention, can see in graphic by those and, the cell culture apparatus designed by the present invention mainly comprises: cell body 10, diaphragm 20, permanent sleeve 30 and a supporting seat 40, wherein:
This cell body 10 is the hollow body of a up/down perforation shape, and a upper shed is formed on cell body 10 top, forms the location division 12 that an opening 11 and is positioned at opening 11 periphery bottom cell body 10.
This diaphragm 20 is the porousness diaphragms with permeability, and this diaphragm 20 is opening parts be covered in bottom this cell body 10 of removable group, and diaphragm 20 periphery extends to the side, location division 12 bottom cell body 10.
This permanent sleeve 30 is on the location division 12 being installed in bottom this cell body 10 of removable group, in order to be entirely fixed on bottom cell body 10 by diaphragm 20.
This supporting seat 40 comprises a pedestal 41, or comprises several feets 42 further, and these several feets are located to downward-extension bottom this pedestal 41, and this supporting seat 40 is mounted on outside this cell body 10, in order to support cell body 10 with this pedestal 41.
As shown in Fig. 1 to Fig. 3 Yu Fig. 5 to Fig. 6, the location division 12 of this cell body 10 is the ring-type Access Divisions 13 forming in cell body 10 bottom periphery face, first preferred embodiment as shown in Figure 1 to Figure 3, this permanent sleeve 30 has the ring-type body 300 that can be sheathed on cell body 10 periphery, the ring root edge 301 extended is formed inwardly in ring-type body 300 bottom, or, second preferred embodiment as shown in Figure 5 to Figure 6, this permanent sleeve 30 has the ring-type body 300 that can be sheathed on cell body 10 periphery, this permanent sleeve 30 forms ring-type Fixed Division 31 in ring-type body 300 inner peripheral surface, ring-type Access Division 13 and ring-type Fixed Division 31 are that the male-female engagement forming arc surface constructs, in the first preferred embodiment shown in Fig. 2 or Fig. 3, ring-type Access Division 13 is the arc surface flange of ring-type, 31, ring-type Fixed Division is the recessed edge design of arc surface of ring-type, in the another kind of preferred embodiment shown in Fig. 4, ring-type Access Division 13a also can be the recessed edge of arc surface of ring-type, ring-type Fixed Division 31a is then the arc surface flange design of ring-type, so, the sheathed cell body 10 that is fixed on of the ring-type body 300 of permanent sleeve 30 can be made peripheral, diaphragm 20 is gripped between ring-type Access Division 13 and ring-type Fixed Division 31, and utilize ring-type Access Division 13 and ring-type Fixed Division 31 to be the male-female engagement of arc surface, make not only there is linking steady character between the two, and there is the function being easy to combine and disassemble, if described permanent sleeve 30 is when ring-type body 300 bottom has ring root edge 301, ring root edge 301 can clamp diaphragm 20 relatively with the location division 12 of cell body 10 bottom further.
The pedestal 41 of this supporting seat 40 is an annular solid, these several feets 42 form in this annular base body 41 root edge to downward-extension, this pedestal 41 also can adjust up and down and positioningly be mounted on outside cell body 10, in preferred embodiment as shown in Figure 2 or Figure 3, this pedestal 41 top forms the inner ring protuberance 43 diametrically extended inwardly, adjustment location division 44 is formed in described inner ring protuberance 43 inner ring periphery, described adjustment location division 44 can be the inner ring periphery that multiple convex grain 441 is spaced in inner ring protuberance 43 and formed, the periphery of this cell body 10 above location division 12 forms the recessed edge 14 in location of several upper and lower spaced ring-type, in order to provide clip location, adjustment location division 44 wherein, make cell body 10 can adjust height by relative support seat about 40, this several ring-type is located position in recessed edge 14 and is located the apical margin of recessed edge 14 close to cell body 10 upper one, in the preferred embodiment shown in Fig. 1 to Fig. 3, supporting seat 40 also can in formation pressure section, inner ring protuberance 43 bottom surface 45, as pressing down the position of pushing permanent sleeve 30.
In preferred embodiment as shown in Figure 1 or 2, this cell body 10 apical margin forms a ring top flange 15, the external diameter of ring top flange 15 is greater than inner ring protuberance 43 internal diameter of supporting seat 40, make cell body 10 from top to bottom through in supporting seat 40, and the inner ring protuberance 43 of only supporting seat 40 and spacing can be supported by ring top flange 15.
Cell culture apparatus of the present invention is in time using, the leaching of this cell culture apparatus is placed in the substratum including nutrient solution and additive ..., separately active somatic cell to be cultivated (or the combination ... of cell and biomedical material .) is attached to suitable and smooth porousness diaphragm 20 surface of permeability, makes active somatic cell be continued survival cultivation in the medium.
As shown in Figure 7, in culturing process, user can utilize supporting seat 40 to adjust the high and low position of cell body 10, the diaphragm 20 of cell body 10 bottom surface is made to be arranged in substratum suitably height location, allow the nutrient solution that comprises in substratum and additive have good permeability in diaphragm 20, make the active somatic cell on diaphragm 20 surface can be subject to good cultivation.
When user is for carrying out cell observation by microscope, annular base body 41 is up adjusted, make the diaphragm 20 bottom cell body 10 can close to or be placed on microscopical Stage microscope, allow user can accurate adjustment microscope focusing, to obtain comparatively ideal micro-imaging.Come to an end in cell cultures, as shown in Figure 8, permanent sleeve 30 can be pulled down from cell body 10 bottom surface, the pressure section 45 of supporting seat 40 wherein can be utilized to press down and to push permanent sleeve 30, and utilize ring-type Access Division 13 to construct with the arc surface shape male-female engagement of ring-type Fixed Division 31, permanent sleeve 30 and cell body 10 are departed from, take out diaphragm 20, the diaphragm 20 be removed can be directly used in clinical cytology and transplant or the transplanting of celliferous biomedical material, or directly observe under being placed in microscopical object lens, so, the supporting seat 40 of cell body 10 and periphery thereof can be avoided microscopical obstruction, this user can focus by accurate adjustment microscope, and obtain comparatively ideal cell image.
In sum, the supporting seat height that cell culture apparatus of the present invention really can solve cell observation time slot body periphery makes microscope cannot the problem of exact focus, and the disassembling operations of cell culture apparatus to diaphragm designed by the present invention is easy, therefore in technical field of cell culture or the technical field such as other biological material and regenerative medicine, a practical cell culture apparatus having industrial utilization really can be provided.
The above description of this invention is illustrative, and nonrestrictive, and those skilled in the art is understood, and can carry out many amendments, change or equivalence, but they all will fall within the scope of protection of the present invention within the spirit and scope of claim restriction to it.
Claims (7)
1. a cell culture apparatus, is characterized in that: it includes:
The cell body of one hollow form, a upper shed is formed on cell body top, forms the location division that an opening and is positioned at this mouth periphery bottom cell body, and this location division is the ring-type Access Division forming in cell body bottom periphery face;
One diaphragm be the opening part be covered in bottom cell body tearing group open, and diaphragm periphery extends to the side, location division bottom cell body;
One permanent sleeve, to tear open on the location division being installed in bottom cell body of group, this permanent sleeve has the ring-type body that is sheathed on cell body periphery, this ring-type body inner peripheral surface forms ring-type Fixed Division, ring-type Access Division and ring-type Fixed Division are the male-female engagement forming arc surface, make that permanent sleeve is sheathed is fixed on cell body periphery, described diaphragm is clamped and fastened between ring-type Access Division bottom cell body and ring-type Fixed Division; And
One supporting seat, comprise a pedestal, the pedestal of this supporting seat has an annular solid, feet is propped up to downward-extension number at annular base body root edge, described pedestal is mounted on outside described cell body and supports cell body, this pedestal top forms the inner ring protuberance diametrically extended inwardly, formed at the inner ring periphery of inner ring protuberance and be spaced formed adjustment location division by multiple convex grain, the periphery of this cell body above location division forms several recessed edge in upper and lower spaced location, locate recessed edge and provide clip location, adjustment location division wherein, the annular seat physical efficiency of described supporting seat is adjusted up and down be positioningly mounted on outside cell body.
2. cell culture apparatus according to claim 1, is characterized in that: form the pressure section that can push against permanent sleeve bottom this pedestal inner ring protuberance.
3. cell culture apparatus according to claim 1 and 2, is characterized in that: ring-type Access Division is the arc surface flange of ring-type, and ring-type Fixed Division is the recessed edge of arc surface of ring-type.
4. cell culture apparatus according to claim 1 and 2, is characterized in that: ring-type Access Division is the recessed edge of arc surface of ring-type, and ring-type Fixed Division is the arc surface flange of ring-type.
5. cell culture apparatus according to claim 1 and 2, is characterized in that: the ring-type body bottom of this permanent sleeve forms the ring root edge extended inwardly, and the ring root edge of permanent sleeve is relative with the location division of cell body bottom clamps diaphragm.
6. cell culture apparatus according to claim 1 and 2, is characterized in that: ring-type Access Division is the arc surface flange of ring-type, and ring-type Fixed Division is the recessed edge of arc surface of ring-type; The ring-type body bottom of this permanent sleeve forms the ring root edge extended inwardly, and the ring root edge of permanent sleeve is relative with the location division of cell body bottom clamps diaphragm.
7. cell culture apparatus according to claim 1 and 2, is characterized in that: ring-type Access Division is the recessed edge of arc surface of ring-type, and ring-type Fixed Division is the arc surface flange of ring-type; The ring-type body bottom of this permanent sleeve forms the ring root edge extended inwardly, and the ring root edge of permanent sleeve is relative with the location division of cell body bottom clamps diaphragm.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2011/000924 WO2012162855A1 (en) | 2011-06-01 | 2011-06-01 | Cell cultivator |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103620018A CN103620018A (en) | 2014-03-05 |
| CN103620018B true CN103620018B (en) | 2015-05-20 |
Family
ID=47258258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201180071316.4A Active CN103620018B (en) | 2011-06-01 | 2011-06-01 | Cell cultivator |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN103620018B (en) |
| WO (1) | WO2012162855A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ITMI20131494A1 (en) * | 2013-09-10 | 2015-03-11 | Univ Pisa | INNOVATIVE SUPPORT FOR CELL CULTURE |
| WO2020001733A1 (en) * | 2018-06-25 | 2020-01-02 | Universitätsklnikum Hamburg-Eppendorf | A holding device for microscopy applications and microscopy chamber |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6057150A (en) * | 1997-09-19 | 2000-05-02 | Bio-Rad Laboratories, Inc. | Biaxial strain system for cultured cells |
| TW200944589A (en) * | 2008-04-18 | 2009-11-01 | Lynn Ling-Hui Huang | Embedded culture tank |
| WO2010045753A1 (en) * | 2008-10-22 | 2010-04-29 | Huang Lynn L H | Flushtype culture tank |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3332991A1 (en) * | 1982-09-13 | 1984-03-15 | Konishiroku Photo Industry Co., Ltd., Tokyo | METHOD FOR FORMING A COLOR DIFFUSION TRANSFER IMAGE BY WARM DEVELOPING |
| US5026649A (en) * | 1986-03-20 | 1991-06-25 | Costar Corporation | Apparatus for growing tissue cultures in vitro |
| EP0590485B1 (en) * | 1992-09-28 | 1998-07-29 | Becton, Dickinson and Company | Cell culture insert |
| US20080076170A1 (en) * | 2006-09-27 | 2008-03-27 | Tuija Annala | Cell culture insert and cell culture vessel |
| CN201195726Y (en) * | 2008-02-19 | 2009-02-18 | 中国航天员科研训练中心 | Combined cell culture bottle |
-
2011
- 2011-06-01 WO PCT/CN2011/000924 patent/WO2012162855A1/en active Application Filing
- 2011-06-01 CN CN201180071316.4A patent/CN103620018B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6057150A (en) * | 1997-09-19 | 2000-05-02 | Bio-Rad Laboratories, Inc. | Biaxial strain system for cultured cells |
| TW200944589A (en) * | 2008-04-18 | 2009-11-01 | Lynn Ling-Hui Huang | Embedded culture tank |
| WO2010045753A1 (en) * | 2008-10-22 | 2010-04-29 | Huang Lynn L H | Flushtype culture tank |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012162855A1 (en) | 2012-12-06 |
| CN103620018A (en) | 2014-03-05 |
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